CN1237632A - Method for preparing rabbitpox culturing tissue containing high-quality physiological activity - Google Patents
Method for preparing rabbitpox culturing tissue containing high-quality physiological activity Download PDFInfo
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- CN1237632A CN1237632A CN98102329A CN98102329A CN1237632A CN 1237632 A CN1237632 A CN 1237632A CN 98102329 A CN98102329 A CN 98102329A CN 98102329 A CN98102329 A CN 98102329A CN 1237632 A CN1237632 A CN 1237632A
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Abstract
The present invention relates to a method for obtaining high-quality pox vaccine with the actions of antianaphylaxis, analgesia and sedation by using various poxviruses. It is characterized by vaccinating rabbit with body weight of 2-3 kg at 50-200 positions for every rabbit,each position uses 0.1-0.4 ml of poxvirus solution with 10 6-10 8 virus/ml by adopting subscutaneous vaccination, and after vaccination for 3-5 days, the pox vaccine can be obtained with high-quality and high yield.
Description
The invention provides and use various poxvirus, can obtain having the good manufacture method that anti-allergic effects, analgesic activity or sedative effect are sent out the acne tissue.
At present existing report, the extract from the skin histology preparation of the rabbit that infected acne poison such as vaccinia virus is effectively for anaphylactic disease, and has also showed sedative effect for the exaltation of autonomic nerve.In addition, also know to have the active substance that hinders Pancreatic Kininogenase and generate, the generation that has suppressed bradykinin that with the Pancreatic Kininogenase kassinin kinin be, a conduct acne material in this material, shown analgesic activity.
In addition, on pharmaceutical journal the 96th volume No. 10 the 1247th～1254 page (1976), also reported from using purified vaccine lymph 00 and the rabbit organization that infects obtains sending out the method for acne tissue.
But, also do not know to obtain the yield height at present, contain the detailed method of sending out the acne tissue of high-quality physiologically active substance.
The objective of the invention is to the various poxvirus of tissue inoculation, a kind of method of obtaining high yield and high-quality acne tissue is provided to rabbit.This acne tissue contains the physiologically active substance that can be used as the medical material use.
Poxvirus among the present invention, can enumerate with cow poxvirus, bull poxvirus, pig pox virus etc. is the virus of the strand virus subfamily class of representative.
In addition, think that also the weight increase of rabbit arrives about 4 kilograms, body weight is heavy more, skin dose also increases more, the receipts amount of then sending out the acne tissue is also many, but the result of research, when body weight surpasses 3.0 kilograms, the increase of sending out the acne tissue is slow, with 2.5 kilograms of its productivitys of the left and right sides for well.
For inoculation method, also can adopt kind of the formula inoculation method of cutting in the past, but the result who studies is seeded in intracutaneous, the receipts amount can increase, and can obtain Pancreatic Kininogenase is generated an acne tissue that hinders active high value.
Postvaccinal acne just can see that from the 1st day all get along with every day usually, and its peak was at the 5th～6 day.Send out taking period of acne tissue, it is generally acknowledged in its peak period best, but should send out acne in the best state before, promptly postvaccinal the 4th day front and back, its receipts amount the best.
Up to now, about for obtain sending out the acne tissue to the quantity of intradermal vaccination still without any report, the result of our research, found that the inoculation position to an animal is 50～200 places, preferably 150 points can make an acne in good condition, in the intracutaneous like this, receipts amount height, and can improve the generation activity that hinders Pancreatic Kininogenase significantly.
In addition, an acne tissue that obtains is even also demonstrate the obstruction activity that Pancreatic Kininogenase is generated after heat sterilization and freezing preservation.
Specifically describe the present invention by the following examples, but the present invention is not subjected to the restriction of these embodiment.
Embodiment 1
The rabbit of 2.5 kilograms healthy mature is anaesthetized with pentobarbital sodium inj, be fixed on the fixer after, wipe the chaeta of rabbit off with electronic hairclipper etc., but will note not injuring skin.
Then, remove the skin of chaeta, after the sterilization, print 150 markings, be separated by about 3 centimetres between per two markings with the scourings such as absorbent cotton that contain 70% ethanolic soln, at marking place, will with phosphate buffered sodium-chlor liquid be mixed with 10
7The vaccinia virus diluent of individual/milliliter is inoculated into intracutaneous with each 0.2 milliliter.
Behind the inoculation vaccinia virus diluent, took off in 4th, after the massacre, take to send out the skin of acne with scissors, scalpel and tweezers from anchor.
In addition, carry out the steam heating sterilising treatment for the skin of taking of sending out acne.
Embodiment 2
The rabbit of 3.0 kilograms healthy mature is anaesthetized with pentobarbital sodium inj, be fixed on the fixer after, wipe the chaeta of rabbit off with electronic hairclipper etc., but will note not injuring skin.
Then, with after containing the sterilization of 70% ethanolic soln and removing the skin of chaeta, with mark seal totally 200 places in addition at about 3 centimetres interval, 107/milliliter the vaccinia virus diluent that will be mixed with phosphate buffered sodium-chlor liquid at this place is inoculated into intracutaneous with each 0.2 milliliter.
Below carry out sterilising treatment in the same manner with embodiment 1 after, obtain sending out an acne tissue.
Embodiment 3
The rabbit of 2.0 kilograms healthy mature is anaesthetized with pentobarbital sodium inj, be fixed on the fixer after, wipe the chaeta of rabbit off with electronic hairclipper etc., but will note not injuring skin.
Then, with after containing the sterilization of 70% ethanolic soln and removing the skin of chaeta,, will be mixed with 10 with phosphate buffered sodium-chlor liquid at this place with mark seal totally 50 places in addition at about 4 centimetres interval
6The vaccinia virus diluent of individual/milliliter is inoculated into intracutaneous with each 0.2 milliliter.The skin of acne is taked to send out in the inoculation back after the 3rd day.
Below carry out sterilising treatment in the same manner with embodiment 1 after, obtain sending out an acne tissue.
Reference example 1
The rabbit and the embodiment 1 of 2.5 kilograms healthy mature are handled in the same manner,, will be mixed with 10 with phosphate buffered sodium-chlor liquid at this place with mark seal totally 30 places in addition at about 3 centimetres interval
7The diluent of individual/milliliter is inoculated into intracutaneous with each 0.2 milliliter.Take off from anchor inoculation back the 4th day, takes to send out the skin of acne after the massacre.The skin of sending out acne for taking carries out the steam heating sterilising treatment in the same manner with embodiment 1.
Reference example 2
The rabbit and the embodiment 1 of 2.5 kilograms healthy mature are handled in the same manner,, will be mixed with 10 with phosphate buffered sodium-chlor liquid at this place with mark seal totally 150 places in addition at about 3 centimetres interval
7The diluent of individual/milliliter is inoculated into intracutaneous with each 0.2 milliliter.Take off from anchor inoculation back the 4th day, takes to send out the skin of acne after the massacre.Below, carry out sterilising treatment in the same manner with reference example 1.
Reference example 3
The rabbit and the embodiment 1 of 2.5 kilograms healthy mature are handled in the same manner,, will be mixed with 10 with phosphate buffered sodium-chlor liquid at this place with mark seal totally 150 places in addition at about 3 centimetres interval
7The diluent of individual/milliliter is inoculated into intracutaneous with each 0.2 milliliter.Inoculate back the 2nd day, take to send out the skin of acne.Below, carry out sterilising treatment in the same manner with reference example 1.
Reference example 4
The rabbit and the embodiment 1 of 2.5 kilograms healthy mature are handled in the same manner,, will be mixed with 10 with phosphate buffered sodium-chlor liquid at this place with mark seal totally 150 places in addition at about 3 centimetres interval
7The diluent of individual/milliliter is with each 0.2 milliliter of inoculation intracutaneous.Inoculate back the 7th day, take to send out the skin of acne.Below, carry out sterilising treatment in the same manner with reference example 1.
The weight of sending out the acne tissue that embodiment 1 to 3 and reference example 1 to 4 obtain and Pancreatic Kininogenase generate the obstruction activity and are illustrated in the table 1.
In addition, the Pancreatic Kininogenase generation active unit of obstruction (1U) is the international unit of Pancreatic Kininogenase.
Table 1
Pancreatic Kininogenase generates
Send out acne tissue weight (g) and hinder active (1U/g) embodiment 1 300 0.45 embodiment 2 290 0.41 embodiment 3 265 0.46 reference examples 1 180 0.18 reference examples 2 195 0.21 reference examples 3 190 0.18 reference examples 4 160 0.13
Claims (2)
1. the rabbit that contains the high quality physiologically active sends out the manufacture method of acne tissue, it is characterized in that whenever sentencing 0.1～0.4 milliliter of inoculation smallpox virus is 10 on the skin of the rabbit of 2～3 kilograms of body weight
6～10
8The solution damping fluid diluent of individual/milliliter amounts to 50～200 places and carries out intradermal vaccination, and the acne tissue is taked to send out in the inoculation back between 3～5 days.
2. the described method of claim 1, poxvirus wherein is that vaccinia virus, poxvirus damping fluid diluent are the phosphate buffered sodium-chlor liquid of vaccinia virus.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB981023290A CN100425692C (en) | 1998-06-02 | 1998-06-02 | Method for preparing rabbitpox culturing tissue containing high-quality physiological activity |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB981023290A CN100425692C (en) | 1998-06-02 | 1998-06-02 | Method for preparing rabbitpox culturing tissue containing high-quality physiological activity |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1237632A true CN1237632A (en) | 1999-12-08 |
| CN100425692C CN100425692C (en) | 2008-10-15 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB981023290A Ceased CN100425692C (en) | 1998-06-02 | 1998-06-02 | Method for preparing rabbitpox culturing tissue containing high-quality physiological activity |
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| CN (1) | CN100425692C (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1557171A1 (en) | 2002-10-31 | 2005-07-27 | VanWorld Pharmaceutical (Rugao) Company Limited | Rabbit skin comprising biological active substance and its use |
| CN105663166A (en) * | 2016-03-02 | 2016-06-15 | 中国人民解放军疾病预防控制所 | Bioactive preparation of specific anti-variola virus infection model strain and application of bioactive preparation |
| US9884077B2 (en) | 2013-04-30 | 2018-02-06 | Nippon Zoki Pharmaceutical Co., Ltd. | Extract and preparation containing said extract |
| US10711292B2 (en) | 2010-10-14 | 2020-07-14 | Nikkon Zoki Pharmaceutical Co., Ltd. | Method for promoting the synthesis of collagen and proteoglycan in chondrocytes |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2651674B2 (en) * | 1987-07-23 | 1997-09-10 | 日本臓器製薬 株式会社 | New physiologically active substance and method for producing the same |
-
1998
- 1998-06-02 CN CNB981023290A patent/CN100425692C/en not_active Ceased
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1557171A1 (en) | 2002-10-31 | 2005-07-27 | VanWorld Pharmaceutical (Rugao) Company Limited | Rabbit skin comprising biological active substance and its use |
| EP1557171A4 (en) * | 2002-10-31 | 2006-08-09 | Vanworld Pharmaceutical Rugao | Rabbit skin comprising biological active substance and its use |
| US10711292B2 (en) | 2010-10-14 | 2020-07-14 | Nikkon Zoki Pharmaceutical Co., Ltd. | Method for promoting the synthesis of collagen and proteoglycan in chondrocytes |
| US9884077B2 (en) | 2013-04-30 | 2018-02-06 | Nippon Zoki Pharmaceutical Co., Ltd. | Extract and preparation containing said extract |
| CN105663166A (en) * | 2016-03-02 | 2016-06-15 | 中国人民解放军疾病预防控制所 | Bioactive preparation of specific anti-variola virus infection model strain and application of bioactive preparation |
| CN105663166B (en) * | 2016-03-02 | 2020-04-17 | 中国人民解放军疾病预防控制所 | Bioactive preparation of specific anti-smallpox virus infection model strain and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN100425692C (en) | 2008-10-15 |
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