CN1235852C

CN1235852C - Method for extracting curcumin from peltate yam

Info

Publication number: CN1235852C
Application number: CNB2003101116420A
Authority: CN
Inventors: 韦进宝
Original assignee: Wuhan University WHU
Current assignee: Wuhan University WHU
Priority date: 2003-12-24
Filing date: 2003-12-24
Publication date: 2006-01-11
Anticipated expiration: 2023-12-24
Also published as: CN1554634A

Abstract

The present invention discloses a method for extracting yellow ginger saponin form yellow ginger. Yellow ginger is washed, pulverized, fermented and hydrolyzed by adding acids; a solid substance (hydrolyzate) and an acidic water solution are centrifuged for separation; the yellow ginger saponin dissolved in the water solution is extracted from the water solution by using butyl acetate; then, the yellow ginger saponin obtained in the solid substance (hydrolysate) is extracted by using the butyl acetate; the yellow ginger saponin is separated out through back extraction. The yellow ginger saponin product can be obtained through further treatment. The present invention has the advantages of simple and convenient method, convenient operation, safety, low cost, energy source saving and wide economic value.

Description

Extract the method for curcumine in the yellow ginger

Technical field

The present invention relates to from plant, extract curcumine, more specifically relate to a kind of method of from yellow ginger, extracting curcumine.

Background technology

Curcumine is a kind of natural pigment, is usually used in candy, ice-creams, soda pop, the cake etc.Mainly Cong Zingiberaceae plant turmeric rhizome, extract.

Yellow ginger is a yam, and its rhizome contains have an appointment 40% starch, 50% fiber and other Chemical Composition.China's yellow ginger mainly originates in ground such as Yunnan, Northwest of Hubei Province, Shan Nan.

Current, people mainly extract saponin from yellow ginger, and other useful component such as curcumine is not utilized, and the anti-refuse that is taken as is discharged in the environment with the waste water of producing saponin.Both wasted resource, again contaminate environment.

Summary of the invention

The object of the present invention is to provide a kind of method of extracting curcumine from yellow ginger, method is easy, and easy to operate, safety, this method are extracted the economic worth that curcumine has improved yellow ginger from yellow ginger, provides a kind of new resource for producing curcumine again.

In order to achieve the above object, the present invention adopts following technical measures: the step of extracting curcumine from yellow ginger is A:(1) yellow ginger is clean through washing; (2) yellow ginger after will washing is pulverized; (3) fermentation of the yellow ginger after will pulverizing; (4) the yellow ginger acid hydrolysis after will fermenting; (5) with acid solids (hydrolyzate) of the yellow ginger centrifugation after the hydrolysis and acidic aqueous solution; (6) aqueous solution extracts curcumine soluble in water with N-BUTYL ACETATE; (7) contain the N-BUTYL ACETATE of curcumine, distill concentratedly, reclaim most N-BUTYL ACETATE (reuse again); (8) will distill mother liquor and be transferred in the beaker, and cool off, leave standstill, curcumine promptly precipitates separates out; (9) filter, oven dry, temperature is controlled at 60-80 ℃, i.e. the curcumine product.

B:(1) yellow ginger is clean through washing; (2) yellow ginger after will washing is pulverized; (3) fermentation of the yellow ginger after will pulverizing; (4) the yellow ginger acid hydrolysis after will fermenting; (5) with acid solids (hydrolyzate) of the yellow ginger centrifugation after the hydrolysis and acidic aqueous solution; (6) solids that centrifugation is obtained (hydrolyzate) extracts with N-BUTYL ACETATE, and the curcumine in the hydrolyzate is extracted into N-BUTYL ACETATE; (7) return collection N-BUTYL ACETATE organic phase with 0.5mol/L sodium hydroxide, curcumine is extracted in the sodium hydroxide solution; (8) return the sodium hydroxide solution of collection, be neutralized to PH5-6 with 6mol/L hydrochloric acid; (9) underpressure distillation concentrates and removes most of water, and gained distilled water is back to the sodium hydroxide solution of preparation 0.5mol/L; (10) be transferred to mother liquor in the beaker with extraction using alcohol, promptly got the ethanolic soln that contains curcumine, residue is Nacl, can be used as Industrial Salt; (11) distillation concentrates, reclaims most ethanol (reuse); (12) mother liquor is transferred in the beaker, cools off, leaves standstill, and curcumine promptly precipitates separates out; (13) remove top solution, precipitation, oven dry, temperature is controlled at 60-80 ℃, promptly gets the curcumine product.From one ton of yellow ginger, can obtain the curcumine of 7-9kg.Sodium hydroxide or the potassium hydroxide solution concentration range of returning collection usefulness are 0.3-1.5mol/L, and recommending with concentration is 0.5mol/L.

The present invention compared with prior art has the following advantages and effect: (1) can be easily to curcumine from yellow ginger, and it is pollution-free to extract curcumine from yellow ginger (yam); (2) extracting is to carry out at normal temperatures and pressures, not only easy but also safety; (3) with low cost, saved the energy, have economic worth widely.

Embodiment

Embodiment 1:

Yellow ginger is through washing, pulverizing, fermentation and acid hydrolysis, after the centrifugation, take by weighing the hydrolyzate of 100 grams after centrifugation, place 250 ml beakers, add 50 milliliters of N-BUTYL ACETATEs, stirred 10 minutes,, then the hydrolyzate in the funnel is reset back in the former beaker to doing with the B suction filtration, added 50 milliliters of N-BUTYL ACETATEs equally, stirred suction filtration 10 minutes.The same processing totally four times.Gained filtrate is put in 500 milliliters of separating funnels, add 100 milliliters of 0.5mol/L sodium hydroxide solutions, vibrated 2 minutes, after leaving standstill 60-90 minute, promptly wait for the clear layering of organic phase and water after, tell water, and then add 100 milliliters of 0.5mol/L sodium hydroxide solutions, return collection more once, after the layering, tell water, twice water merged and place 500 ml beakers, be neutralized to PH5 with 6mol/L hydrochloric acid, the most of water of evaporative removal extracts for four times with 120 milliliters of branches of 95% ethanol, filter with qualitative filter paper for the last time, all ethanol extracts are put in the glass distiller, and distillation concentrates and reclaims most of ethanol, reuses.To distill mother liquor and be transferred in 100 ml beakers, and cool off, leave standstill, the curcumine precipitation is separated out, and removes top solution, with 20 milliliters of ethyl alcohol recrystallizations once, the gained throw out, oven dry, bake out temperature is 60 ℃ or 65 ℃ or 70 ℃, promptly gets curcumine.

The aqueous solution of getting centrifugation places 250 milliliters of separating funnels for 100 milliliters, adds 30 milliliters of N-BUTYL ACETATEs, vibrates 2 minutes, left standstill 30-60 minute, wait clear layering after, remove water, organic phase is put in the glass distiller, and distillation concentrates and reclaims most of N-BUTYL ACETATE.Mother liquor is transferred in 50 ml beakers, cools off, leaves standstill, and curcumine promptly precipitates separates out, and removes top solution, precipitation, and oven dry, bake out temperature is 68 ℃ or 70 ℃ or 75 ℃, promptly gets curcumine.

Embodiment 2:

Yellow ginger is through washing, pulverizing, fermentation and acid hydrolysis, after the centrifugation, takes by weighing solid hydrolyzate 500 grams after the centrifugation, place 1000 ml beakers, 200 milliliters of N-BUTYL ACETATEs of each adding stirred 10 minutes, used the B suction filtration, extract altogether four times, gained filtrate is concentrated in 2000 milliliters of separating funnels, add 100 milliliters of 1.0mol/L sodium hydroxide solutions, vibrated 2 minutes, leave standstill promptly treated that two phase stratification was clear in 60-90 minute after, tell water.Return extraction equally once.The water merging of telling for 2 times places 500 ml beakers, be neutralized to PH6 with 6mol/L hydrochloric acid, after most of water is removed in evaporation, cooling, extract for four times with 400 milliliters of branches of 95% ethanol, filter with qualitative filter paper for the last time, the ethanol extract merging of filtrate and front also places the glass distiller, most of ethanol is reclaimed in distillation, reuses.Mother liquor is transferred in 100 ml beakers, cools off, leaves standstill, and curcumine promptly precipitates separates out, and removes top solution, with 40 milliliters of ethyl alcohol recrystallizations once.Precipitation, oven dry, bake out temperature is 70 ℃ or 75 ℃ or 80 ℃, promptly gets the curcumine product.

The acidic aqueous solution of getting centrifugation places 1000 milliliters of separating funnels for 500 milliliters, adds 150 milliliters of N-BUTYL ACETATEs, vibrates 2 minutes, leave standstill treated clear layering in 30-60 minute after, remove water, organic phase is put in the glass distiller, and most of N-BUTYL ACETATE is reclaimed in distillation.Mother liquor is transferred in 100 ml beakers, cools off, leaves standstill, and precipitation is separated out complete back (about 10 hours), removes top solution, precipitation, and oven dry, bake out temperature is 64 ℃ or 66 ℃ or 74 ℃ or 78 ℃, promptly gets the curcumine product.

Claims

1, extracts the method for curcumine in a kind of yellow ginger, comprise the following steps:

A, yellow ginger are through washing only;

B, the yellow ginger after will washing are pulverized;

C, the fermentation of the yellow ginger after will pulverizing;

D, the yellow ginger acid hydrolysis after will fermenting;

E, with acid solids of the yellow ginger centrifugation after the hydrolysis and acidic aqueous solution;

F, the solids that centrifugation is obtained extract with N-BUTYL ACETATE, and the curcumine in the hydrolyzate is extracted into N-BUTYL ACETATE;

G, return collection N-BUTYL ACETATE organic phase with 0.5mol/L sodium hydroxide, curcumine is extracted in the sodium hydroxide solution;

H, return the sodium hydroxide solution of collection, be neutralized to PH5-6 with 6mol/L hydrochloric acid;

I, underpressure distillation concentrate to remove and anhydrate, and gained distilled water is back to the sodium hydroxide solution of preparation 0.5mol/L;

J, be transferred to mother liquor in the beaker with extraction using alcohol

K, distillation concentrate, reclaim ethanol;

L, mother liquor are transferred in the beaker, cool off, leave standstill, and precipitation is separated out;

M, removal top solution, precipitation, oven dry, temperature is controlled at 60-80 ℃.