CN1227542C - Microbe tracing method for oil deposit well - Google Patents
Microbe tracing method for oil deposit well Download PDFInfo
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- CN1227542C CN1227542C CN 03129322 CN03129322A CN1227542C CN 1227542 C CN1227542 C CN 1227542C CN 03129322 CN03129322 CN 03129322 CN 03129322 A CN03129322 A CN 03129322A CN 1227542 C CN1227542 C CN 1227542C
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- microorganism
- water
- spike
- well
- oil reservoir
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Abstract
The present invention relates to a microbe tracing method between oil deposit wells, which comprises the following steps: water quality analysis of formation water and autochthon microbe analysis of formation water are carried out; microbes which can maintain lives in modes of spores or other hypopus under the environment of an oil reservoir are screened from the existing aerobic or facultative anaerobes to serve as tracing microbes, and at least one item of three parameters, such as thallus form, colonial morphology or growth temperature is different from that of autochthon aerobic or facultative anaerobes of formation water; the microbes are injected into the oil reservoir from a water injecting well; the characteristic of the oil reservoir and the flow parameters of injected water are analyzed according to test results in the corresponding sampling detection of a production well. The present invention takes a nontoxic and pollution-free microbe as a tracer, the existing water filling device of an oil field is used for injection, and a simple microscope or a counting method of plate cultivation are used for detection. The present invention which is a scientific, economic and reliable tracing method between oil deposit wells has the advantages of convenient construction, low cost, wide detection ranges, high sensitivity, no harm to persons and environment, etc.
Description
Technical field
The present invention relates to a kind of oilfield exploitation method, be specifically related to the tracing method in the oilfield exploitation method.
Background technology
The primary oil recovery stage that the general at first experience of oilfield exploitation utilizes the blowing of oil reservoir self-energy to produce, as time passes, the continuous consumption of producing energy finally can not blowing production, at this moment adopt generally that water filling keeps producing energy in oil reservoir, enter secondary phase of oil production thus.Through once, secondary oil recovery, only can the original crude oil reserve of extraction oil reservoir about 30%, substantial oil is retained in the oil reservoir.The nonuniformity of oil reservoir is one of reason that causes this situation.Because the nonuniformity of oil reservoir, cause and inject water at first in the high oil reservoir channelling of permeability, form " fingering " phenomenon, in oil reservoir, form serious water stream channel behind the breakthrough of injection water, a large amount of crude oil " bypass " in will being in, in the low permeability layer falls, and greatly reduces to inject ripples and efficient.At " bypass " problem of injecting water, developed multinomial technical method at present, as adjusting oil reservoir intake profile etc.For science more, use these technical methods effectively and improve flood effectiveness, must involve situation to the flow direction, the volume that injects water in the oil reservoir, the reservoir connected relation has clearly understanding between parameter such as flowing velocity, oil reservoir nonuniformity, oil reservoir high permeability band and the volume size thereof on vertically and well relatively.Tracer technique then is the practicable technology that obtains this understanding between well.
Tracer technique mainly is that tracer agent is injected oil reservoir by water injection well between well, tracer agent is migrated at oil reservoir with injecting water, by the producing well output, reservoir connected relation between parameter such as the flow direction of injecting water in the oil reservoir, flowing velocity, oil reservoir nonuniformity, high permeability band and the volume size thereof on vertically and well is carried out analysis and judgement according to the feature of tracer agent output.In addition, in water injection well, inject distributivity tracer agent and non-distributivity tracer agent simultaneously,, obtain two kinds of tracer agent response curves in producing well sampling, monitoring, according to tracer agent chromatography stalling characteristic and chromatographic theory in oil reservoir, can analyze definite remaining oil saturation at last.
Said tracer agent is meant and is soluble in carrier (as water) or is scattered in the carrier (as water), with the carrier migration, but information such as the flow direction of reaction carriers, flow velocity, material that can be detected under low concentration.At present, the tracer agent that is used for the oil field mainly comprises 4 classes: 1. radioactive isotope: HTO (water tracer), tritiate pentane, tritiate hexane, tritiate heptane, tritiate octane, tritiate benzene, tritiate toluene (oily tracer agent), tritiated hydrogen, tritiated methane (gaseous tracer), the butanols of tritiate butanols, tritiate amylalcohol, carbon-bearing-14 (profit distribution tracer agent) etc.; 2. the negative ion that easily detects: CNS
-, NO
3 -, Br
-, I
-3. dyestuff: plain red, the dawn Y of carmine, mattress, dibromophenolphthalein, lemon yellow etc.; 4. low mass molecule alcohol: methyl alcohol, ethanol, isopropyl alcohol, butanols, amylalcohol etc.
Above-mentioned tracer agent has obtained some application on the oil field, in use find to exist some shortcomings: need special department to throw in and detect as radioisotope tracer; Dye tracer is big in the surface of stratum adsorbance, and it is many to analyze disturbing factor, and instability lost efficacy when the residence time surpasses 5 days in the stratum; Low mass molecule alcohol tracer agent biological stability is poor, must use with germifuge; Anionic inorganic salt tracer agent consumption big (5%-10%) can pollute local water or cause the mineralising of local water, wherein iodide ion price height (about 120,000 yuan per ton of NaI) or the like.In addition, some tracer agents also exist simultaneously to be had harm, financial cost height, detection technique means complexity, need expensive detection equipment, environment is had pollution or the like human body, and this has further limited their application.
Summary of the invention
The technical issues that need to address of the present invention be disclose a kind of with microorganism as trace method between the well of tracer agent, to overcome above-mentioned a series of defectives that prior art exists.
Method of the present invention comprises the steps:
(1) analysis of the aerobic and amphimicrobe thalli morphology of local water water analysis and local water original inhabitants, colonial morphology, growth temperature;
(2) in existing aerobic or amphimicrobe screening at least in thalli morphology, colonial morphology or three parameters of growth temperature one aerobic with the local water original inhabitants and amphimicrobe is different, can gemma under reservoir media or the microorganism of the form maintenance life of other hypopus as the spike microorganism;
(3) with injection water said spike microbial fermentation solution being diluted to volumetric concentration is 4%-10%, is injected in the oil reservoir by water injection well, and spike microbial fermentation solution injection rate IR is 5-20m
3/ well;
The consumption of spike microbial fermentation solution depends primarily on factors such as the adsorbance of the test characteristic on stratum such as factor of porosity, water saturation, nonuniformity etc., microorganism rock surface in the stratum and injector producer distance.List of references Brigham W E, et al.SPE1130,1965 disclosed chemical tracer consumption computing method (Brigham and Smith formula) are considered the specific seepage characteristic of spike microorganism in porous medium, and it is 5-20m that every mouthful of test pit injects spike microbial fermentation liquid measure
3Comparatively suitable, output spike this moment microorganism concn peak value about 10
3-10
4Cells/ml can conveniently detect, and the responding range of broad is arranged again.
Said spike microorganism comprises following microorganism, but is not limited to these microorganisms:
Document: Nazina T N, Ivanova A E, Mityushina L L, et al.Thermophilichydrocarbon-oxidizing bacteria from oil fields.Mikrobiologiya, 62 (3), 583～592,1993 disclosed happiness heat are bitten oily bacillus (B.thermoleovorans), in causing, Peng Qian, Ma Jun, Chen Junying. the research of Yunnan hot spring high temperature bacterium: the extreme thermophilic bud pole bacterium in the acid high temperature hot spring of VII. Tengchong. microorganism journal 29 (3), 161-165,1989 disclosed bacillus stearothermophilus (B.stearothermophilus) and documents: Jenneman G E, et al.a halotolerant biosurfactant-producing bacillus species potentially useful for enhanced oil recovery.Development in industrial microbiology, 25,485-492,1983, Brant R S, DouglasJ.Evaluation of microbial systems in porous media for EOR.SPERE (may1988), 489-495 and Yakimov M M, Amro M M, Bock M, et al.The potential ofBacillus licheniformis strains in situ enhanced oil recovery.J.Pet.Sci.Eng., 18 (1/2), 147-160,1997 disclosed bacillus licheniformis (B.licheniformis), document: Gurerra-Santos L, Kappeli O, Fiechter A.Pseudomonas aeruginosabiosurfactant production in continuous culture with glucose as carbon source.Appl.Environ.Microbiol.48,301-305,1984 and Desai J D, Banat I M.Microbial production of surfactants and their commercial potential.Microbiol.Mol.Biol.Rev., 61 (1), 47-64,1997 disclosed pseudomonas aeruginosas (P.aeruginosa), document: Makkar R S, Cameotra S S.Biosurfactant production by a thermophilicBacillus subtilis strain.J.Ind.Microbiol.Biotechnol., 18 (1), 37-42,1997 disclosed bacillus subtilises (B.subtilis), document: Kim D S, Fogler H S.The effects ofexopolymers on cell morphology and culturability of Leuconostocmesenteroides during starvation.Appl.Microbiological Biotechnology, 52 (6), 839-844,1999 disclosed Leuconostoc mesenteroides (L.mesenteroides), document: Belyaev S S, Borzenkov I A, Milekhina E I, et al.Halotolerant and extremely halophilic oil-oxidizing bacteria in oil fields.Dev.Pet.Sci., 39 (Microbial Enhancement ofOil Recovery:Recent Advances), 79-88,1993 disclosed salt bacillus (H.distribution), document: Milekhina E I, Borzenkov I A, Zvyagintseva I S, et al.Characterization of a hydrocarbon-oxidizing Rhodococcus erythropolis strainisolated from an oil field.Microbiology, 67 (3), 271-274, a kind of in the 1998 disclosed Rhodococcus erythropolis bacterium such as (R.erythropolis) preferably likes heat and bites oily bacillus or bacillus stearothermophilus.
(4) sample analysis:
After in oil reservoir, injecting the spike microorganism by water injection well, can gather output liquid sample at producing well, sampling period can be as requested measuring accuracy, reference test well group water filling breakthrough time, the water filling data such as dynamic change of being benefited determines and optimizes that the general sample period is 1-5 days; Behind the sample collecting, in 24 hours, detect, can adopt the microscopic examination thalli morphology, utilize the dull and stereotyped concentration that counting method is observed colonial morphology and determined the spike microorganism of cultivating, utilize the different growth temperatures of microorganism comparatively to cultivate the spike microorganism of injecting in selectivity ground, can use or unite the use said method separately in the testing process.Distinguish in the feature of stratum indigenous microorganism according to the spike microorganism of injecting, can easily both be distinguished, obtain to inject the output information of spike microorganism.
According to the output information of the spike microorganism of injecting, analyze between parameter such as the flow direction of injecting water in the oil reservoir, flowing velocity, oil reservoir nonuniformity, high permeability band and the volume size thereof on vertically and well reservoir connected relation etc.
The microorganism trace method is a tracer agent with nontoxic, free of contamination microorganism between the oil reservoir well that the present invention proposes, utilize the former water filling device in oil field to inject, adopt easy microscope or the dull and stereotyped counting method of cultivating to detect (need not large-scale analytical equipment and complicated detection means), by fluid produced by oil production well is detected in real time, obtain the information of spike microorganism output, can make analysis and judgement to the flow parameter of reservoir characteristics and injection water thus.Therefore, the present invention is a tracing method between the oil reservoir well of a kind of not only science but also economic and reliable, has easy construction, the source of goods is wide, cost is low, sensing range is wide by (10
0Cells/ml～10
9Cells/ml), highly sensitive, easy to detect, do not need large-scale checkout equipment, to people and advantage such as environmentally friendly.
Embodiment
Embodiment 1
28, on April of certain oil field, inject radioisotope tracer (15 Curie) by water injection well I, to determine to inject the water seepage direction, during April to November, 6 mouthfuls of producing wells to this water injection well correspondence are monitored, the P2 well was seen tracer agent after 52 days as a result, calculated percolation flow velocity: 6.25m/d; The P6 well was seen tracer agent in 69 days, calculated percolation flow velocity: 3.5m/d.Other 4 mouthfuls of producing wells there is no tracer agent.As seen, the main seepage direction of water injection well I injection water is P2 and P6 well direction.
After 3 years, injecting microorganism at the I well, is bacillus stearothermophilus because bacterial classification injects in institute, does not contain the indigenous bacterium of the type in the oil reservoir, so has carried out testing easily according to this feature.Inject preceding 3 days, inject and to gather output liquid sample and analyze at the producing well of correspondence in back 26 days, 67 days, the sample of gathering in back 67 days in injection detects and injects microorganism, P2 well concentration is 50cells/ml, the P6 well is 200cells/ml.By this calculating, percolation flow velocity is about 3.6m/d.
Claims (1)
1. microorganism tracing method between an oil reservoir well is characterized in that, comprises the steps:
(1) analysis of the aerobic and amphimicrobe thalli morphology of local water water analysis and local water original inhabitants, colonial morphology, growth temperature;
(2) in existing aerobic or amphimicrobe screening at least in thalli morphology, colonial morphology or three parameters of growth temperature one aerobic with the local water original inhabitants or amphimicrobe is different, can gemma under reservoir media or the microorganism of the form maintenance life of other hypopus as the spike microorganism;
(3) with injection water said spike microbial fermentation solution being diluted to volumetric concentration is 4%-10%, is injected in the oil reservoir by water injection well, and spike microbial fermentation solution injection rate IR is 5-20m
3/ well;
(4) sample analysis:
After injecting the spike microorganism by water injection well in oil reservoir, can gather output liquid sample at producing well, the sample period is 1-5 days; Behind the sample collecting, in 24 hours, detect, adopt the microscopic examination thalli morphology, utilize the dull and stereotyped concentration that counting method is observed colonial morphology and determined the spike microorganism of cultivating, utilize the different growth temperatures of microorganism comparatively to cultivate the spike microorganism of injecting in selectivity ground, can use or unite the use said method separately in the testing process, distinguish in the feature of stratum indigenous microorganism according to the spike microorganism of injecting, can easily both be distinguished, obtain to inject the output information of spike microorganism;
Said spike microorganism comprises that liking heat bites oily bacillus (B.thermoleovorans), bacillus stearothermophilus (B.stearothermophilus), bacillus licheniformis (B.licheniformis), pseudomonas aeruginosa (P.aeruginosa), bacillus subtilis (B.subtilis), Leuconostoc mesenteroides (L.mesenteroides), salt bacillus (H.distribution), a kind of in Rhodococcus erythropolis (R.erythropolis) bacterium.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB200510107077XA CN1313618C (en) | 2003-06-17 | 2003-06-17 | Application of microorganism as tracer |
| CN 03129322 CN1227542C (en) | 2003-06-17 | 2003-06-17 | Microbe tracing method for oil deposit well |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03129322 CN1227542C (en) | 2003-06-17 | 2003-06-17 | Microbe tracing method for oil deposit well |
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| CNB200510107077XA Division CN1313618C (en) | 2003-06-17 | 2003-06-17 | Application of microorganism as tracer |
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| CN1469134A CN1469134A (en) | 2004-01-21 |
| CN1227542C true CN1227542C (en) | 2005-11-16 |
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| CNB200510107077XA Expired - Fee Related CN1313618C (en) | 2003-06-17 | 2003-06-17 | Application of microorganism as tracer |
| CN 03129322 Expired - Fee Related CN1227542C (en) | 2003-06-17 | 2003-06-17 | Microbe tracing method for oil deposit well |
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| GB2438216B (en) * | 2006-05-17 | 2008-11-19 | Schlumberger Holdings | Methods and systems for evaluation of hydrocarbon reservoirs and associated fluids using biological tags and real-time polymerase chain reactions |
| CN101430387B (en) * | 2007-11-06 | 2011-05-04 | 核工业北京地质研究院 | Nano-scale uranium particulate detection method based on soil gaseous water |
| CN102517377B (en) * | 2011-11-19 | 2014-04-02 | 胜利油田胜利勘察设计研究院有限公司 | Method for detecting and analyzing biomembrane on inner wall of oil field sewage pipe |
| CN108458957B (en) * | 2017-02-21 | 2022-06-17 | 中国石油化工股份有限公司 | Device and method for simulating water rock reaction |
| CN111254056B (en) * | 2020-02-26 | 2023-03-21 | 中国科学院深海科学与工程研究所 | Deep sea biogeochemical in-situ experiment device |
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| US7934833B2 (en) * | 2005-12-22 | 2011-05-03 | Alcon Refractivehorizons, Inc. | Image alignment system for use in laser ablation treatment of the cornea |
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| CN1757747A (en) | 2006-04-12 |
| CN1469134A (en) | 2004-01-21 |
| CN1313618C (en) | 2007-05-02 |
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