CN1210122A - Extraction of blue pigment and polysaccharide from spirulina and use of residues - Google Patents
Extraction of blue pigment and polysaccharide from spirulina and use of residues Download PDFInfo
- Publication number
- CN1210122A CN1210122A CN 97109227 CN97109227A CN1210122A CN 1210122 A CN1210122 A CN 1210122A CN 97109227 CN97109227 CN 97109227 CN 97109227 A CN97109227 A CN 97109227A CN 1210122 A CN1210122 A CN 1210122A
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- algae
- residue
- blue pigment
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- spirulina
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Abstract
A process for extracting blue phycochrome, polyose and its dregs includes such steps as adding phosphoric acid buffering liquid (5-20 times in volume) to dry spirulina powder, repeated freeze-thaw, centrifugal separation, collecting supernatant, freeze drying to obtain dried blue phycochrome, adding water (2-6 times in volume) to deposit, water bath at 80 deg.C for 4 hrs, centrifugal separation, drying the deposit to obtain dregs as food additive, collecting supernatant, adding industrial alcohol (3-4 time in volume), centrifugal separation and freeze drying to obtain dried polyose.
Description
The present invention relates to the comprehensive utilization of spirulina, particularly from spirulina, extract algae blue pigment and polysaccharide and algae-residue thereof as foodstuff additive.
Algae blue pigment can improve lymphoid activity, and enhancing body immunizing power is antitumor, promotes the propagation of hematopoietic cell and medullary cell, and the damage that significantly alleviates Radiation on Mouse peripheral blood cells and medullary cell has good health care function and auxiliary curative effect.The spirulina soluble polysaccharide has enhance immunity power, radioprotective and antitumous effect.Except that as the pharmaceuticals, algae blue pigment is of wide application as water miscible cyanine, for the additive of various luxury foods, not only color is gorgeous, and health-care effect is arranged.China's spiral factory developed rapidly in recent years, the algae powder yield sharply increases, does not open as yet the foreign market, and the domestic market product is single, and market development is ineffective, the algae powder overstocks in a large number, China's spirulina industry develops rapidly under the situation that is subjected to serious challenge, and the algae powder is carried out deep processing, makes product diversification, to adapt to the demand of domestic market, become pressing for of numerous spirulina companies.People such as Cui Junyi " from spirulina, extract the method and the application thereof of protein and polysaccharide) " patent applied for, application number: 94112454, notification number: 1106414, this patent is mainly extracted protein and polysaccharide from spirulina, wherein algae blue pigment extracts not exclusively and in the proteins extraction process and is subjected to certain destruction, and the algae-residue of carrying behind finished white matter and the polysaccharide is utilized.
The objective of the invention is to extract algae blue pigment and polysaccharide and algae-residue utilization thereof from spirulina, carry algae blue pigment earlier, carry polysaccharide again, remaining algae-residue has been realized the comprehensive utilization of spirulina as foodstuff additive.
To achieve these goals, the present invention is by the following technical solutions: the phosphoric acid buffer (0.1M that adds 5-20 times of volume in the spirulina dry algae powder, PH6.8), multigelation 2-8 time, centrifugal, collect the supernatant liquor lyophilize and get the algae blue pigment dry product, add 2-6 times of volume water to precipitation again, 80 ℃ of water-baths 4 hours, centrifugal, drying precipitated algae-residue is as foodstuff additive; Collect supernatant liquor simultaneously, 60 ℃ of water-baths concentrate, and add excessive Pb (Ac) again
2Deproteinize, centrifugal, collect supernatant liquor, add decolorizing with activated carbon, centrifugal, add the industrial spirit of 3-4 times of volume again to supernatant liquor, centrifugal, lyophilize precipitate the polysaccharide dry product.Wherein the algae blue pigment yield is 29.56%, and the soluble polysaccharide yield is 0.2758%, residue algae-residue nutrition such as following table:
| Form content | Protein (%) | Palmitinic acid (mgg) | Palm diluted acid mgg | Stearic acid mgg | Oleic acid mgg | Linolic acid mgg | Linolenic acid mgg | Fe mgg | Mg ％ | Ca ％ |
| 70.5 | ?17.14 | ?1.37 | ?0.500 | 0.48 | ?3.39 | ?7.94 | ?416.5 | ?0.20 | 0.22 |
Effect of the present invention is: utilize spirulina powder to be raw material, extract algae blue pigment earlier, carry polysaccharide again, remaining algae-residue has been realized the comprehensive utilization of spirulina as foodstuff additive, has reduced cost, has improved added value, is suitable for batch production production.
Embodiment:
Get 500g spirulina dry algae powder and add 2.5-10L phosphoric acid buffer (0.1M, PH6.8), stir evenly, place-20 ℃ of refrigerators, take out after solution freezes, melt under the 5-25 ℃ of temperature in dark place, so freeze thawing is 2-6 time, centrifugal (6000rpm * 20min), collect blue supernatant liquor, lyophilize gets the algae blue pigment dry product; Add 3-9L water to precipitation again, 80 ℃ of water-baths 4 hours, centrifugal (6000rpm, 15min), drying precipitated algae-residue dry product; Collect supernatant liquor simultaneously, 60 ℃ of water-baths concentrate spends the night, and adds Pb (Ac) again
2Deproteinize, and use Na
2CO
3(1000g algae powder adds 4.5NPb (AC) to take off lead
2, 4NNa
2CO
3), it is centrifugal that (6000rpm 15min), collects supernatant liquor, add decolorizing with activated carbon (the 1L supernatant liquor adds 4g), centrifugal (6000rpm 15min), adds the industrial spirit of 3-4 times of volume again to supernatant liquor, centrifugal, (6000rpm, 15min), lyophilize precipitate the polysaccharide dry product; Dried algae-residue adds 5-15 times of distilled water freeze thawing 1 time, adds in the 5-20% ratio and makes all kinds drink in the different beverages; Dried algae-residue adds 3-15 times of distilled water freeze thawing 1 time, adds oral liquid in the 3-15% ratio and makes oral liquid, and dried algae-residue is pulverized the back adds increase food in cake, noodles, the instant noodles by the 10-30% ratio egg day matter and the content of unsaturated fatty acids.
Claims (4)
1, from spirulina, extract algae blue pigment and polysaccharide and algae-residue thereof and use, it is characterized in that adding phosphoric acid buffer to the spirulina dry algae powder earlier, multigelation, centrifugal, the lyophilize supernatant liquor gets the algae blue pigment dry product; Add water to precipitation again, water-bath, centrifugal, the drying precipitated algae-residue that gets; Collect supernatant liquor simultaneously, concentrate, Deproteinization, the processing industry alcohol precipitation, centrifugal, lyophilize precipitate the polysaccharide dry product.
2, the phosphoric acid buffer (0.1M that from spirulina, extracts algae blue pigment and polysaccharide and algae-residue application thereof, it is characterized in that in the spirulina dry algae powder, adding 5-20 times of volume according to claim 1, PH6.8), place-20 ℃ of refrigerator multigelations 2-8 time, centrifugal (6000rpm * 20min), collect blue supernatant liquor, lyophilize gets the algae blue pigment dry product.
3, the algae blue pigment and polysaccharide and algae-residue thereof of extracting from spirulina according to claim 1 used, it is characterized in that the precipitation of having carried algae blue pigment adds 2-6 times of volume water, 80 ℃ of water-baths 4 hours, centrifugal (6000rpm, 15min), collect supernatant liquor simultaneously, 60 ℃ of water-baths concentrate spends the night, and adds Pb (Ac) again
2Deproteinize, and use Na
2CO
3(1000g algae powder adds 4.5NPb (Ac) to take off lead
24NNa
2CO
3), centrifugal (6000rpm 15min), adds the industrial spirit of 3-4 times of volume again to supernatant liquor, centrifugal, (6000rpm, 15min), lyophilize precipitate the polysaccharide dry product.
4, the algae blue pigment and polysaccharide and algae-residue thereof of extracting from spirulina according to claim 1 used, it is characterized in that having put forward the precipitation behind the algae blue pigment, add water, water-bath, centrifuged deposit, the dry algae-residue dry product that gets, dried algae-residue adds 5-15 times of distilled water freeze thawing 1 time, adds in the 5-20% ratio and makes all kinds drink in the different beverages; Dried algae-residue adds 3-15 times of distilled water freeze thawing 1 time, adds oral liquid in the 3-15% ratio and makes oral liquid; Dried algae-residue is pulverized the back and is added protein and the unsaturated fatty acid content that increases food in cake, noodles, the instant noodles in the 10-30% ratio.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN97109227A CN1072248C (en) | 1997-09-04 | 1997-09-04 | Extraction of blue pigment and polysaccharide from spirulina and use of residues |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN97109227A CN1072248C (en) | 1997-09-04 | 1997-09-04 | Extraction of blue pigment and polysaccharide from spirulina and use of residues |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1210122A true CN1210122A (en) | 1999-03-10 |
| CN1072248C CN1072248C (en) | 2001-10-03 |
Family
ID=5171037
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN97109227A Expired - Fee Related CN1072248C (en) | 1997-09-04 | 1997-09-04 | Extraction of blue pigment and polysaccharide from spirulina and use of residues |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1072248C (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100413929C (en) * | 2004-12-10 | 2008-08-27 | 西北工业大学 | Method of extracting natural yellow pigment from persimon leaf or persion bark |
| CN102433015A (en) * | 2011-10-14 | 2012-05-02 | 陈勇 | Method for preparing blue-green algae pigment |
| CN103947913A (en) * | 2014-05-13 | 2014-07-30 | 高天荣 | Method for reducing harmful elements in spiral seaweeds |
| CN108409827A (en) * | 2018-05-21 | 2018-08-17 | 福州大学 | A method of synchronous extraction protein and polysaccharide from microalgae algae-residue |
| CN111909811A (en) * | 2020-07-27 | 2020-11-10 | 深圳市太空微藻生物科技有限公司 | Preparation method and product of algae-flavor liquor |
| CN113476891A (en) * | 2021-08-06 | 2021-10-08 | 中国科学院南海海洋研究所 | Method for simultaneously extracting multiple active substances from porphyridium |
| CN116814487A (en) * | 2023-06-26 | 2023-09-29 | 复旦大学 | Low-sugar high-protein spirulina dreg and preparation method and application thereof |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100408642C (en) * | 2006-04-29 | 2008-08-06 | 大连大学 | Method for integrated extraction of active substances in Platymonas |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1030346A (en) * | 1987-07-04 | 1989-01-18 | 中国科学院感光化学研究所 | From marine alga, extract the method for natural food colour |
-
1997
- 1997-09-04 CN CN97109227A patent/CN1072248C/en not_active Expired - Fee Related
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100413929C (en) * | 2004-12-10 | 2008-08-27 | 西北工业大学 | Method of extracting natural yellow pigment from persimon leaf or persion bark |
| CN102433015A (en) * | 2011-10-14 | 2012-05-02 | 陈勇 | Method for preparing blue-green algae pigment |
| CN102433015B (en) * | 2011-10-14 | 2014-11-12 | 陈勇 | Method for preparing blue-green algae pigment |
| CN103947913A (en) * | 2014-05-13 | 2014-07-30 | 高天荣 | Method for reducing harmful elements in spiral seaweeds |
| CN108409827A (en) * | 2018-05-21 | 2018-08-17 | 福州大学 | A method of synchronous extraction protein and polysaccharide from microalgae algae-residue |
| CN111909811A (en) * | 2020-07-27 | 2020-11-10 | 深圳市太空微藻生物科技有限公司 | Preparation method and product of algae-flavor liquor |
| CN113476891A (en) * | 2021-08-06 | 2021-10-08 | 中国科学院南海海洋研究所 | Method for simultaneously extracting multiple active substances from porphyridium |
| CN116814487A (en) * | 2023-06-26 | 2023-09-29 | 复旦大学 | Low-sugar high-protein spirulina dreg and preparation method and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1072248C (en) | 2001-10-03 |
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