CN1208077A - Unicellular protein feed producing technology using stillage material - Google Patents
Unicellular protein feed producing technology using stillage material Download PDFInfo
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- CN1208077A CN1208077A CN98117426A CN98117426A CN1208077A CN 1208077 A CN1208077 A CN 1208077A CN 98117426 A CN98117426 A CN 98117426A CN 98117426 A CN98117426 A CN 98117426A CN 1208077 A CN1208077 A CN 1208077A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Fodder In General (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention produces single-cell protein (SCP) feed with three-strain bacteria and solid stillage material and through no-sterilization energy-saving technology. Through the culture of three-strain bacteria in incubator at 28-30 deg.c for 24 hr in liquid culture medium inside triangular bottle to obtain the first stage spawn; pot culture at 28-30 deg.c for 24 hr to obtain the second stage spawn; inoculation to stillage material for solid fermentation at 90%RH and 28-30 deg.c for 40 hr; drying at 50 deg.c, crushing and packing, the feed is produced.
Description
The present invention relates to a kind of poor slag raw material production SCP feed technology.
At present, the SCP feed is given birth to by single bacterium or two bacterium association, and half sterilization or the solid kind fermentation culture technology of sterilizing are entirely produced.Cause product big energy-consuming, cost height like this.
The object of the present invention is to provide poor slag raw material three bacterium, the unsterilised power-saving technology of liquid secondary kind solid material, thereby reduce production costs, increase economic efficiency.
Poor slag raw material production SCP feed technology of the present invention, successively according to following process production:
Three strain bacterium are inoculated into respectively in the slant medium (YPG), put 28～30 ℃ of constant temperature culture and promptly got slant strains in 48 hours; Three strain slant strains being transferred to triangular flask respectively puts 28～30 ℃ and cultivates and promptly to get the level liquid kind in 24 hours again; Again the one-level kind respectively is inoculated in the Ka Shi jar by 1%, putting 28～30 ℃ cultivated 24 hours, then by BAS-201: BAS-208: BAS-226=6: mixed was inoculated in the unsterilised poor slag solid medium in 2: 2, send relative humidity 90%, 28～30 ℃ of workshops cultivations of temperature to after 40 hours, 55 ℃ of following humidity dryings, pulverize, obtain a kind of poor slag raw material production SCP feed of the present invention.
Described bacterial strain preferred yeast bacterium (Yeasts) is provided by culture presevation portion of the special bio-engineering research of Beijing agriculture and animal husbandry institute.Preserving number: BAS-201, BAS-208 and BAS-226.
Described bacterial classification usage ratio is 201: 208: 226=6: 2: 2.
Described slant strains substratum production process is as follows successively:
5 gram yeast extract pastes, 5 gram peptones and 5 gram glucose add and add 18 gram agar after the 1000 ml distilled waters dissolvings and supply 1000 milliliters to boiling, and transfer 1.1 kilograms per centimeter in 20 * 200 millimeters test tubes of pH 6.5 packing
2Sterilized 30 minutes, and took out and put into the slant strains substratum.
Described triangular flask level liquid kind production process is as follows successively:
Starch 3, cake class 2, water 97 are transferred pH 6.5 dress triangular flasks 1.1 kilograms per centimeter
2Sterilize and obtained the one-level kind in 30 minutes.
Described Ka Shi jar substratum production process is as follows successively:
Starch 3, cake class 2, water 97, the heating of pH 6.5 mixings obtain Ka Shi jar substratum for 100 ℃.
Poor, slag solid medium production process is as follows successively:
Poor slag 90, bran 8, inorganic salt 2, water 100 mixings after vexed 2 hours, connect 2 grades of kind 10% dosage, put 28～30 ℃ and cultivate 40 hours, and 50 ℃ of following drying and crushing get product of the present invention.
Described product is rich in high egg, VITAMIN amino acid, multiple enzyme.
Poor slag raw material production SCP fodder production technology of the present invention has following advantage
1. nutritive ingredient is abundant; 2. energy-conservation, reduce cost.3. market outlook are good.
Claims (6)
1. poor, slag raw material production single cell protein (SCP) feed technology, successively according to following process production:
Three strain bacterium are received respectively in the substratum (YPE), 28～30 ℃ are cultivated after 48 hours is slant strains, again slant strains is received and gone up shaking table in the triangular flask liquid nutrient medium, cultivated 24 hours for 28～30 ℃, then triangular flask one-level kind is pressed in the Ka Shi jar by 1% dosage, cultivated 24 hours for 28～30 ℃, as the secondary kind, receive in poor, the slag solid fermentation substratum by 10% inoculum size, putting 28～30 ℃ cultivated 40 hours, dry below 50 ℃, pulverize and obtain a kind of poor slag raw material production SCP feed of the present invention.
2. according to the production technology of claim 1, it is characterized in that used BAS-201, BAS-208 and BAS-226 bacterial classification.
3. according to claim 1 production technology, it is characterized in that bacterial classification BAS-201: BAS-208: BAS-226=6: 2: 2.
4. according to claim 1, its feature adopts unsterilised production.
5. according to claim 1, production technology liquid strain, solid culture.
6. according to claim 1, temperature is controlled 28～30 ℃.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN98117426A CN1208077A (en) | 1998-08-21 | 1998-08-21 | Unicellular protein feed producing technology using stillage material |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN98117426A CN1208077A (en) | 1998-08-21 | 1998-08-21 | Unicellular protein feed producing technology using stillage material |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1208077A true CN1208077A (en) | 1999-02-17 |
Family
ID=5225514
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN98117426A Pending CN1208077A (en) | 1998-08-21 | 1998-08-21 | Unicellular protein feed producing technology using stillage material |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1208077A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101965900A (en) * | 2010-09-27 | 2011-02-09 | 黑龙江八一农垦大学 | Method for producing probiotic single cell protein by using soybean molasses |
| US9725742B2 (en) | 2012-05-10 | 2017-08-08 | Abengoa Bioenergy New Technologies, Llc | High efficiency ethanol process and high protein feed co-product |
-
1998
- 1998-08-21 CN CN98117426A patent/CN1208077A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101965900A (en) * | 2010-09-27 | 2011-02-09 | 黑龙江八一农垦大学 | Method for producing probiotic single cell protein by using soybean molasses |
| CN101965900B (en) * | 2010-09-27 | 2012-08-15 | 黑龙江八一农垦大学 | Method for producing probiotic single cell protein by using soybean molasses |
| US9725742B2 (en) | 2012-05-10 | 2017-08-08 | Abengoa Bioenergy New Technologies, Llc | High efficiency ethanol process and high protein feed co-product |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |