CN1207309C - Method for extracting ganoderma lucidum spore polysaccharide - Google Patents
Method for extracting ganoderma lucidum spore polysaccharide Download PDFInfo
- Publication number
- CN1207309C CN1207309C CNB031318371A CN03131837A CN1207309C CN 1207309 C CN1207309 C CN 1207309C CN B031318371 A CNB031318371 A CN B031318371A CN 03131837 A CN03131837 A CN 03131837A CN 1207309 C CN1207309 C CN 1207309C
- Authority
- CN
- China
- Prior art keywords
- polysaccharide
- ganoderma spore
- hour
- extracting
- particle
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/54—Improvements relating to the production of bulk chemicals using solvents, e.g. supercritical solvents or ionic liquids
Abstract
The present invention relates to a method for extracting ganoderic spore polysaccharide. The present invention is characterized in that a CO2 supercritical extraction method is used for extracting ganoderic spore oil for other application firstly, and then the residue obtained after the ganoderic spore oil is processed by degreasing, aqueous extraction, alcohol precipitation, filtration and drying so as to obtain the ganoderic spore polysaccharide.
Description
Technical field
The present invention relates to from Chinese medicinal herbs, extract the extracting method of the method, particularly a kind of ganoderma spore polysaccharide of physiologically active substance.
Background technology
Have physiologically active substance can be divided into two big classes as the traditional method of macromolecule polysaccharide, triterpenes, organic germanium, alkaloid etc. be ethanol extract from water precipitation or water extraction and alcohol precipitation method from extracting the Chinese medicinal herbs, but if ganoderma spore polysaccharide is by above-mentioned traditional method for extracting, can make material quantity excessive, because Ganoderma spore is too tiny, cause difficulty can for the filtration operation in the leaching process simultaneously.Being problematic in that of its maximum contains 30% lipid composition in the Ganoderma spore, when adopting traditional water to carry, a large amount of oil substances are separated out, and can produce interference to extracting polysaccharide, and the finished product are difficult to avoid the residual of oil substances, the foreign matter content height, and yield is low.
Summary of the invention
The extracting method that the purpose of this invention is to provide a kind of ganoderma spore polysaccharide, this method is being used disclosed CO
2In the remaining slag charge, further adopt water extraction to obtain ganoderma spore polysaccharide behind the supercritical fluid extraction Ganoderma spore oil.
Above-mentioned purpose of the present invention is achieved in that
A kind of extracting method of ganoderma spore polysaccharide is characterized in that using earlier CO
2The supercritical fluid extraction Ganoderma spore oil carries out to carrying residuum behind the oil that degreasing, water are carried, alcohol precipitation, filtration, drying then, ganoderma spore polysaccharide.The concrete steps of extracting polysaccharide are:
A. with making powder behind the Ganoderma spore raw material broken wall, add additive then and make particle, use CO after the seasoning
2Supercritical extraction deoils, and gets degreasing Ganoderma spore particle; B. the extractor of particle being packed into adds water 3-30 doubly, soaks 0.5-5 hour, is heated to and boils, maintenance backflow 1-10 hour; C. with extracting solution centrifuging or filtering under pressure, make solid-liquid separation, get clarification filtration liquid, the dregs of a decoction decoct 1-3 time more in accordance with the law, merge filtered liquid; D. clear liquor is concentrated into every milliliter and is equivalent to crude drug 1-2 gram, adds the 95% edible ethanol precipitation polysaccharide of 3-20 times of weight, leaves standstill 4-48 hour; E. leach oven dry behind the polysaccharide, spraying or lyophilize and get the solid ganoderma spore polysaccharide.
The present invention has following advantage and effect:
1, adopts the Ganoderma spore powder of degreasing, it is convenient that polysaccharide is extracted, a large amount of oil substances are separated out when avoiding water to carry, thereby obtain highly purified polysaccharide, polysaccharide content is up to about 60%, as adopt technologies such as macroporous resin adsorption, and also can further improve polysaccharide content, degreasing Ganoderma spore gained polysaccharide content only is about 25% and traditional aqueous extraction-alcohol precipitation technology adopts not.
2, agents useful for same of the present invention is nontoxic, can not inject new objectionable impurities to product, and extraction effect is good, and ethanol can constantly be recycled simultaneously, has further reduced production cost.
3, technical process of the present invention is simple, and cost is low, and equipment interoperability is strong.
4, described ganoderma spore polysaccharide can add some other component, and as fungus polysaccharide, support one's family and usually improve its nourishing function and therapeutic action, the ganoderma spore polysaccharide that adds or do not add other component can further be processed into capsule or tablet, conveniently to take.
5, use CO
2The grease obtained class material of supercritical extraction is Ganoderma spore oil, include a large amount of triterpenes functional components, can add oil-soluble inhibitor, also can add some other component, as Oleum Hippophae, support one's family and usually improve its nourishing function and therapeutic action, the Ganoderma spore oil that adds or do not add other component can further be processed into soft capsule or drops, conveniently to take.
6, ganoderma spore polysaccharide has antitumor action, immunoregulation effect, hypoglycemic activity, antioxygenation and anti-aging effects etc.Show that in the preliminary experimentation on animals of Jiangsu Prov. Tumour Hospital mouse S180, the strain of Herps knurl are had good inhibition effect with gained spore polysaccharide sample of the present invention, tumour inhibiting rate is about 40～60%.
Embodiment
Embodiment 1
Ganoderma spore powder is added the mixture of entry, gelatin or wherein a kind of additive, and add-on is as the criterion the Ganoderma spore powder molding bonded, and mixing is made particle, and CO is used in seasoning
2Supercritical extraction is removed oil substances wherein.Take by weighing degreasing Ganoderma spore 10kg, in the extractor of packing into, add 15 times of water, soaked 2 hours, and be heated to and boil, keep refluxing 4 hours, extracting solution is centrifugal, clarification filtration liquid is concentrated into 5kg, adds 3 times of weight 95% edible ethanols, leaves standstill 24 hours, make polysaccharide precipitation, filter, oven dry gets ganoderma spore polysaccharide 122g.
Embodiment 2
Identical with embodiment 1 operating process, different is, extraction conditions soaked 2 hours for adding 20 times in water, is heated to and boils, and keeps refluxing 4 hours, adds 3 times of weight 95% edible ethanols precipitation polysaccharide after concentrating, oven dry, ganoderma spore polysaccharide 130g.
Embodiment 3
Identical with embodiment 2, different is that amount of water is 4 times, the final ganoderma spore polysaccharide 102g that gets.
Embodiment 4
Identical with embodiment 2, different is that amount of water is 30 times, the final ganoderma spore polysaccharide 133g that gets.
Embodiment 5
Identical with embodiment 2, different is, add water after direct heating to boiling, keep little and boiled 4 hours, final ganoderma spore polysaccharide 107g.
Embodiment 6
Identical with embodiment 2, different is to keep little and boiled 1 hour, the final ganoderma spore polysaccharide 65g that gets.
Embodiment 7
Identical with embodiment 2, different is to keep little and boiled 8 hours, the final ganoderma spore polysaccharide 135g that gets.
Embodiment 8
Identical with embodiment 2, different is to add 1 times of weight 95% edible ethanol precipitation polysaccharide, the final ganoderma spore polysaccharide 27g that gets.
Embodiment 9
Identical with embodiment 2, different is to add 8 times of weight 95% edible ethanol precipitation polysaccharide, the final ganoderma spore polysaccharide 138g that gets.
Embodiment 10
Identical with embodiment 2, different is, with leaving standstill after the edible ethanol 4 hours, and final ganoderma spore polysaccharide 53g.
Embodiment 11
Identical with embodiment 2, different is, with leaving standstill after the edible ethanol 36 hours, and final polysaccharide 137g.
Embodiment 12
Identical with embodiment 2, different is to adopt spray-dired method when dry, the final ganoderma spore polysaccharide 128g that gets.
Embodiment 13
Identical with embodiment 2, different is to adopt cryodesiccated method when dry, the final ganoderma spore polysaccharide 131g that gets.
Claims (2)
1. the extracting method of a ganoderma spore polysaccharide is characterized in that using earlier CO
2The supercritical fluid extraction Ganoderma spore oil carries out to carrying residuum behind the oil that degreasing, water are carried, alcohol precipitation, filtration, drying then, ganoderma spore polysaccharide.
2. according to the extracting method of the described ganoderma spore polysaccharide of claim 1, it is characterized in that:
A. with making powder behind the Ganoderma spore raw material broken wall, add additive then and make particle, use CO after the seasoning
2Supercritical extraction deoils, and gets degreasing Ganoderma spore particle;
B. the extractor of particle being packed into adds water 3-30 doubly, soaks 0.5-5 hour, is heated to and boils, maintenance backflow 1-10 hour;
C. with extracting solution centrifuging or filtering under pressure, make solid-liquid separation, get clarification filtration liquid, the dregs of a decoction decoct 1-3 time more in accordance with the law, merge filtered liquid;
D. clear liquor is concentrated into every milliliter and is equivalent to crude drug 1-2 gram, adds the 95% edible ethanol precipitation polysaccharide of 3-20 times of weight, leaves standstill 4-48 hour;
E. leach oven dry behind the polysaccharide, spraying or lyophilize and get the solid ganoderma spore polysaccharide.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB031318371A CN1207309C (en) | 2003-06-10 | 2003-06-10 | Method for extracting ganoderma lucidum spore polysaccharide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB031318371A CN1207309C (en) | 2003-06-10 | 2003-06-10 | Method for extracting ganoderma lucidum spore polysaccharide |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1483743A CN1483743A (en) | 2004-03-24 |
| CN1207309C true CN1207309C (en) | 2005-06-22 |
Family
ID=34153892
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB031318371A Expired - Fee Related CN1207309C (en) | 2003-06-10 | 2003-06-10 | Method for extracting ganoderma lucidum spore polysaccharide |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1207309C (en) |
Families Citing this family (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1854158B (en) * | 2005-04-25 | 2011-09-07 | 蒋来高 | Extraction of astragalus mongholicus polysaccharose by super-critical fluid technology |
| MX2008012066A (en) * | 2006-03-23 | 2008-10-07 | Herbalscience Singapore Pte Ltd | Extracts and methods comprising ganoderma species. |
| CN100424098C (en) * | 2006-10-25 | 2008-10-08 | 南京中科集团股份有限公司 | Process for refining glossy ganoderma spore polysaccharide |
| CN101317649B (en) * | 2007-06-05 | 2013-05-08 | 深圳市汇康生物科技有限公司 | Method for preparing glossy ganoderma spore oil |
| CN102206291B (en) * | 2011-04-11 | 2012-10-31 | 北京林业大学 | Process for extracting yam polysaccharide by using supercritical CO2 fluid extraction technique |
| CN103445138B (en) * | 2013-07-03 | 2016-01-06 | 广州市浩立生物科技有限公司 | A kind of Chinese caterpillar fungus bacterium powder tablet or capsule and preparation method thereof |
| CN103690573B (en) * | 2013-12-25 | 2017-03-15 | 南京中科药业有限公司 | A kind of natural drug composition for strengthen immunity and preparation method thereof |
| CN104798948A (en) * | 2014-01-28 | 2015-07-29 | 齐鲁工业大学 | Preparation method of peony stamen instant tea |
| CN104387495A (en) * | 2014-12-14 | 2015-03-04 | 桂林宏旺菌业股份有限公司 | Preparation method of ganoderma lucidum polysaccharide |
| CN105175564B (en) * | 2015-08-21 | 2018-01-16 | 仙芝科技(福建)股份有限公司 | The industrialization extraction separation method and separator of ganoderma spore polysaccharide |
| CN105837700A (en) * | 2016-04-12 | 2016-08-10 | 江苏江大源生态生物科技股份有限公司 | A comprehensive extraction, utilization and production method for glossy ganoderma sporocarps and spores |
| CN106173779A (en) * | 2016-08-23 | 2016-12-07 | 杨育平 | A kind of Ganoderma spore solid beverage and preparation method thereof |
| CN106620284A (en) * | 2016-12-29 | 2017-05-10 | 广州市芝元生物科技有限公司 | Completely-wall-broken ganoderma spore powder rich in spore oil |
| CN107158043A (en) * | 2017-04-10 | 2017-09-15 | 浙江寿仙谷医药股份有限公司 | One kind removes wall lucidum spore powder, particle and preparation method thereof |
| CN108623703B (en) * | 2018-05-29 | 2020-12-11 | 金华寿仙谷药业有限公司 | Method for preparing ganoderma lucidum spore powder exine crude polysaccharide by irradiation |
| CN109400741B (en) * | 2018-11-06 | 2021-05-18 | 中科健康产业集团江苏药业有限公司 | Separation and purification method of ganoderma lucidum spore polysaccharide |
| CN111087489A (en) * | 2020-01-15 | 2020-05-01 | 安徽祥云谷现代中药有限公司 | CO (carbon monoxide)2Method for extracting polysaccharide from wall-broken ganoderma lucidum spore powder residue after supercritical extraction of spore oil |
| CN112546078B (en) * | 2020-11-26 | 2022-08-30 | 中科健康产业集团股份有限公司 | Ganoderma lucidum spore extract and application thereof |
| CN112898444B (en) * | 2021-02-07 | 2023-01-10 | 上海百信生物科技有限公司 | Method for preparing muramyl polysaccharide by using ganoderma lucidum spore powder raffinate, product and application thereof |
| CN114631598A (en) * | 2022-04-09 | 2022-06-17 | 吉林省爱康寿生物科技有限公司 | Non-waste preparation treatment process for industrialization of cut-log ganoderma lucidum |
| CN116035149A (en) * | 2022-12-28 | 2023-05-02 | 南京中科药业有限公司 | Preparation method of ganoderma lucidum solid beverage |
-
2003
- 2003-06-10 CN CNB031318371A patent/CN1207309C/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN1483743A (en) | 2004-03-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN1207309C (en) | Method for extracting ganoderma lucidum spore polysaccharide | |
| CN101249116B (en) | Waxberry polyphenol chromocor extract, and preparations and applications thereof | |
| CN1699586A (en) | Process for extracting tea polyphenol, caffeine as a byproduct thereof and tea polysaccharide from tea | |
| CN101032533A (en) | Method of preparing solution / substance extracted from ginkgo leaves | |
| CN101274953B (en) | Method for extracting corosolic acid from plant | |
| CN101747446A (en) | Method for extracting anti-fatigue acidic ginseng polysaccharide | |
| CN1958555A (en) | Method for preparing salviol acid A | |
| CN110054560A (en) | A kind of method of purification and its application of pharmaceutical woody alpha-linolenic acid | |
| CN107033045B (en) | A kind of preparation method of high-purity natural garlic 4,5,9-trithiadodeca-1,6,11-triene 9-oxide | |
| CN102432573B (en) | Method for preparing lovastatin | |
| CN101638404B (en) | High-purity salvianolic acid B and preparation method and application thereof | |
| CN1211378C (en) | Method for preparing ginkgo leaf extractive | |
| CN1313435C (en) | Novel method for extracting 4-hydroxy isoleucine product from trigonella | |
| CN1817874A (en) | Method for removing caffeine from theapolyphenol coarse extract | |
| CN1740137A (en) | Chlorogenic acid extracting and purifying process from sunflower seed dregs | |
| CN1120723C (en) | Health-care lucid qanoderma product and its preparing process | |
| CN104232314A (en) | Preparation method and health product of platycodon grandiflorum seed essential oil | |
| CN1931199A (en) | Double resin process for preparing high quality active gingko leaf component extract | |
| CN1830458A (en) | New use of tribulus terrestris extraction | |
| CN1730027A (en) | Method for preparing anti-hepatitis active part from swertia main pharmaceutical plant | |
| CN1543876A (en) | Novel monascin oral liquid and method for producing the same | |
| CN1943647A (en) | The method for preparing triterpenic acid extract from the loquat leaves | |
| CN105061458B (en) | Preparation method of nontoxic high-purity ginkgo total lactones and monomers thereof | |
| CN1587271A (en) | Process for preparing morinda officinalis total oligosaccharide | |
| CN1483734A (en) | Novel process for extracting puerarin from pueraria mirifica |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20050622 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |