CN120019149A - 共聚聚羟基烷酸酯混合物的制造方法及转化微生物 - Google Patents

共聚聚羟基烷酸酯混合物的制造方法及转化微生物 Download PDF

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Publication number
CN120019149A
CN120019149A CN202380070963.6A CN202380070963A CN120019149A CN 120019149 A CN120019149 A CN 120019149A CN 202380070963 A CN202380070963 A CN 202380070963A CN 120019149 A CN120019149 A CN 120019149A
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China
Prior art keywords
gene
pha
polyhydroxyalkanoate
strain
microorganism
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Pending
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CN202380070963.6A
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English (en)
Chinese (zh)
Inventor
有川尚志
加藤纱也
毛利佳弘
佐藤俊辅
杉山博昭
西森盐穗美
西中智哉
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Kaneka Corp
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Kaneka Corp
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Publication of CN120019149A publication Critical patent/CN120019149A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/62—Carboxylic acid esters
    • C12P7/625—Polyesters of hydroxy carboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/01—Hydro-lyases (4.2.1)
    • C12Y402/01017—Enoyl-CoA hydratase (4.2.1.17), i.e. crotonase

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN202380070963.6A 2022-10-03 2023-09-26 共聚聚羟基烷酸酯混合物的制造方法及转化微生物 Pending CN120019149A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2022-159239 2022-10-03
JP2022159239 2022-10-03
PCT/JP2023/034999 WO2024075597A1 (ja) 2022-10-03 2023-09-26 共重合ポリヒドロキシアルカン酸混合物の製造方法、及び形質転換微生物

Publications (1)

Publication Number Publication Date
CN120019149A true CN120019149A (zh) 2025-05-16

Family

ID=90608187

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202380070963.6A Pending CN120019149A (zh) 2022-10-03 2023-09-26 共聚聚羟基烷酸酯混合物的制造方法及转化微生物

Country Status (5)

Country Link
US (1) US20250230474A1 (https=)
EP (1) EP4600360A1 (https=)
JP (1) JPWO2024075597A1 (https=)
CN (1) CN120019149A (https=)
WO (1) WO2024075597A1 (https=)

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2777757B2 (ja) 1991-09-17 1998-07-23 鐘淵化学工業株式会社 共重合体およびその製造方法
JPH07265065A (ja) 1994-03-29 1995-10-17 Kanegafuchi Chem Ind Co Ltd 共重合体の合成遺伝子による形質転換体および共重合体の製造方法
CN101297036B (zh) 2005-10-27 2013-03-13 株式会社钟化 新质粒载体和稳定保持质粒的转化体
JP2007259708A (ja) 2006-03-27 2007-10-11 Kaneka Corp 新規生分解性ポリエステルの製造方法
WO2010067543A1 (ja) 2008-12-09 2010-06-17 株式会社カネカ ポリ－３－ヒドロキシアルカン酸の製造方法およびその凝集体
EP2540835B1 (en) 2010-02-26 2018-05-09 Tokyo Institute of Technology PROCESS FOR PRODUCTION OF POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYHEXANOATE) USING A GENETICALLY MODIFIED CUPRIAVIDUS NECATOR HAVING AN ENOYL-CoA HYDRATASE GENE INTRODUCED THEREIN
EP3101129B1 (en) 2014-01-31 2020-10-28 Kaneka Corporation Microorganism having adjusted expression of r-specific enoyl-coa hydratase gene, and method for manufacturing polyhydroxyalkanoate copolymer using same
EP3124615B1 (en) * 2014-03-28 2021-11-10 Kaneka Corporation Microorganism having multiple genes encoding pha synthase and method for producing pha using same
EP3358002B1 (en) * 2015-09-28 2024-08-28 Kaneka Corporation Microorganism having pha synthase-coding genes and method for producing pha using same
US11186831B2 (en) * 2018-01-16 2021-11-30 Kaneka Corporation Mutant polyhydroxyalkanoate synthase, gene thereof and transformant, and method for producing polyhydroxyalkanoate
JP7270556B2 (ja) 2018-01-17 2023-05-10 株式会社カネカ 高組成比率の３ｈｈモノマー単位を含む共重合ｐｈａを生産する形質転換微生物およびそれによるｐｈａの製造方法
WO2021206155A1 (ja) * 2020-04-10 2021-10-14 株式会社カネカ 共重合ポリヒドロキシアルカン酸混合物の製造方法、及び形質転換微生物
JP7781749B2 (ja) 2020-06-09 2025-12-08 株式会社カネカ ポリヒドロキシアルカン酸の製造方法およびその利用

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Publication number Publication date
JPWO2024075597A1 (https=) 2024-04-11
EP4600360A1 (en) 2025-08-13
WO2024075597A1 (ja) 2024-04-11
US20250230474A1 (en) 2025-07-17

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