CN119365606A - 未分化多能干细胞的检测方法和检测试剂 - Google Patents

未分化多能干细胞的检测方法和检测试剂 Download PDF

Info

Publication number
CN119365606A
CN119365606A CN202380045888.8A CN202380045888A CN119365606A CN 119365606 A CN119365606 A CN 119365606A CN 202380045888 A CN202380045888 A CN 202380045888A CN 119365606 A CN119365606 A CN 119365606A
Authority
CN
China
Prior art keywords
seq
cells
pluripotent stem
cell
stem cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202380045888.8A
Other languages
English (en)
Chinese (zh)
Inventor
长船健二
辻本啓
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Raj Nefono Co ltd
Kyoto University NUC
Original Assignee
Raj Nefono Co ltd
Kyoto University NUC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Raj Nefono Co ltd, Kyoto University NUC filed Critical Raj Nefono Co ltd
Publication of CN119365606A publication Critical patent/CN119365606A/zh
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6881—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN202380045888.8A 2022-06-10 2023-06-09 未分化多能干细胞的检测方法和检测试剂 Pending CN119365606A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2022094396 2022-06-10
JP2022-094396 2022-06-10
PCT/JP2023/021545 WO2023238932A1 (ja) 2022-06-10 2023-06-09 未分化多能性幹細胞の検出方法および検出試薬

Publications (1)

Publication Number Publication Date
CN119365606A true CN119365606A (zh) 2025-01-24

Family

ID=89118426

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202380045888.8A Pending CN119365606A (zh) 2022-06-10 2023-06-09 未分化多能干细胞的检测方法和检测试剂

Country Status (5)

Country Link
US (1) US20250376727A1 (https=)
EP (1) EP4538384A1 (https=)
JP (1) JPWO2023238932A1 (https=)
CN (1) CN119365606A (https=)
WO (1) WO2023238932A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN119979719A (zh) * 2025-02-26 2025-05-13 中国食品药品检定研究院 一种基于基因的多能干细胞残留通用型检测方法

Family Cites Families (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9453219B2 (en) 2003-05-15 2016-09-27 Mello Biotech Taiwan Co., Ltd. Cosmetic designs and products using intronic RNA
BRPI0619794B8 (pt) 2005-12-13 2022-06-14 Univ Kyoto Uso de um fator de reprogramação, agente para a preparação de uma célula-tronco pluripotente induzida a partir de uma célula somática e métodos para preparar uma célula- tronco pluripotente induzida método e para preparar uma célula somática e uso de células-tronco pluripotentes induzidas
DK3399025T3 (da) 2007-03-23 2025-06-16 Wisconsin Alumni Res Found Omprogrammering af somatiske celler
JP2008307007A (ja) 2007-06-15 2008-12-25 Bayer Schering Pharma Ag 出生後のヒト組織由来未分化幹細胞から誘導したヒト多能性幹細胞
AU2008297011B2 (en) 2007-08-31 2014-06-12 Whitehead Institute For Biomedical Research Wnt pathway stimulation in reprogramming somatic cells
AU2008297024B2 (en) 2007-10-31 2014-08-28 Kyoto University Nuclear reprogramming method
JP5558097B2 (ja) 2007-12-10 2014-07-23 国立大学法人京都大学 効率的な核初期化方法
US20100330063A1 (en) 2007-12-17 2010-12-30 Gliamed, Inc. Stem-like cells, method for de-differentiating mammalian somatic cells into stem-like cells, and method for differentiating stem-like cells
WO2009091659A2 (en) 2008-01-16 2009-07-23 Shi-Lung Lin Generation of tumor-free embryonic stem-like pluripotent cells using inducible recombinant rna agents
EP2250252A2 (en) 2008-02-11 2010-11-17 Cambridge Enterprise Limited Improved reprogramming of mammalian cells, and the cells obtained
EP2090649A1 (en) 2008-02-13 2009-08-19 Fondazione Telethon Method for reprogramming differentiated cells
US20110014164A1 (en) 2008-02-15 2011-01-20 President And Fellows Of Harvard College Efficient induction of pluripotent stem cells using small molecule compounds
SG188904A1 (en) 2008-03-17 2013-04-30 Scripps Research Inst Combined chemical and genetic approaches for generation of induced pluripotent stem cells
WO2009114949A1 (en) 2008-03-20 2009-09-24 UNIVERSITé LAVAL Methods for deprogramming somatic cells and uses thereof
AU2009234424A1 (en) 2008-04-07 2009-10-15 Nupotential, Inc. Reprogramming a cell by inducing a pluripotent gene through use of an HDAC modulator
CA2695590C (en) 2008-06-27 2018-01-09 Kyoto University Method of efficiently establishing induced pluripotent stem cells
EP2313494A2 (en) 2008-07-14 2011-04-27 Oklahoma Medical Research Foundation Production of pluripotent cells through inhibition of bright/arid3a function
WO2010147612A1 (en) 2009-06-18 2010-12-23 Lixte Biotechnology, Inc. Methods of modulating cell regulation by inhibiting p53
WO2010033920A2 (en) 2008-09-19 2010-03-25 Whitehead Institute For Biomedical Research Compositions and methods for enhancing cell reprogramming
WO2010033906A2 (en) 2008-09-19 2010-03-25 President And Fellows Of Harvard College Efficient induction of pluripotent stem cells using small molecule compounds
WO2010042800A1 (en) 2008-10-10 2010-04-15 Nevada Cancer Institute Methods of reprogramming somatic cells and methods of use for such cells
WO2010050626A1 (en) 2008-10-30 2010-05-06 Kyoto University Method for producing induced pluripotent stem cells
WO2010056831A2 (en) 2008-11-12 2010-05-20 Nupotential, Inc. Reprogramming a cell by inducing a pluripotent gene through use of an hdac modulator
US9045737B2 (en) 2008-12-13 2015-06-02 Dnamicroarray, Inc. Artificial three-dimensional microenvironment niche culture
JP5553289B2 (ja) 2009-02-27 2014-07-16 国立大学法人京都大学 新規核初期化物質
WO2010102267A2 (en) 2009-03-06 2010-09-10 Ipierian, Inc. Tgf-beta pathway inhibitors for enhancement of cellular reprogramming of human cells
WO2010111409A2 (en) 2009-03-25 2010-09-30 The Salk Institute For Biological Studies Pluripotent stem cells
WO2010111422A2 (en) 2009-03-25 2010-09-30 The Salk Institute For Biological Studies Induced pluripotent stem cell generation using two factors and p53 inactivation
US8852940B2 (en) 2009-04-01 2014-10-07 The Regents Of The University Of California Embryonic stem cell specific microRNAs promote induced pluripotency
US20110088107A1 (en) 2009-04-24 2011-04-14 Yaqub Hanna Compositions and methods for deriving or culturing pluripotent cells
WO2010147395A2 (en) 2009-06-16 2010-12-23 Korea Research Institute Of Bioscience And Biotechnology Medium composition comprising neuropeptide y for the generation, maintenance, prologned undifferentiated growth of pluripotent stem cells and method of culturing pluripotent stem cell using the same
US9115345B2 (en) * 2010-04-19 2015-08-25 The Trustees Of The University Of Pennsylvania MicroRNA induction of pluripotential stem cells and uses thereof
JP2013158325A (ja) 2012-02-08 2013-08-19 Hokkaido Univ 細胞診断方法
WO2016104717A1 (ja) 2014-12-26 2016-06-30 国立大学法人京都大学 肝細胞誘導方法
WO2020213734A1 (ja) 2019-04-19 2020-10-22 国立大学法人京都大学 ネフロン前駆細胞の製造方法
EP4386083A4 (en) 2021-08-11 2025-11-12 Univ Kyoto METHOD FOR PRODUCING RENAL INTERSTITIAL PROGENITOR CELLS, ERYTHROPOIETIN-PRODUCING CELLS AND METHOD FOR PRODUCING RENIIN-PRODUCING CELLS

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN119979719A (zh) * 2025-02-26 2025-05-13 中国食品药品检定研究院 一种基于基因的多能干细胞残留通用型检测方法
CN119979719B (zh) * 2025-02-26 2026-01-13 中国食品药品检定研究院 一种基于基因的多能干细胞残留通用型检测方法

Also Published As

Publication number Publication date
JPWO2023238932A1 (https=) 2023-12-14
US20250376727A1 (en) 2025-12-11
EP4538384A1 (en) 2025-04-16
WO2023238932A1 (ja) 2023-12-14

Similar Documents

Publication Publication Date Title
Kaur et al. Small non-coding RNA landscape of extracellular vesicles from human stem cells
Abranches et al. Neural differentiation of embryonic stem cells in vitro: a road map to neurogenesis in the embryo
Freeman et al. Single-cell RNA-Seq of bone marrow-derived mesenchymal stem cells reveals unique profiles of lineage priming
JP6493881B2 (ja) 人工多能性幹細胞の選別方法および血球への分化誘導方法
KR102784455B1 (ko) 재프로그래밍 벡터
WO2013188679A1 (en) Methods of preparing pluripotent stem cells
HK1258309A1 (zh) 用於确定候选药物的功效特徵的方法
Horisawa et al. Direct cell-fate conversion of somatic cells: Toward regenerative medicine and industries
WO2010108126A2 (en) Reprogramming compositions and methods of using the same
EP3121276B1 (en) Cardiomyocyte sorting method
Jönsson et al. Comprehensive analysis of microRNA expression in regionalized human neural progenitor cells reveals microRNA-10 as a caudalizing factor
US20220401493A1 (en) Isolation of fusion-competent myoblasts and therapeutic applications thereof related to muscular dystrophy
EP2524033B1 (en) Method for screening induced pluripotent stem cells
CN113355433A (zh) 一种基于单细胞测序数据分析的iPSC残留检测方法
US10604770B2 (en) Method for extracting differentiated cells
Pessôa et al. Generation and miRNA characterization of equine induced pluripotent stem cells derived from fetal and adult multipotent tissues
JP7173496B2 (ja) 心筋細胞への分化指向性を有する多能性幹細胞を選抜するための方法
US20230340595A1 (en) Use of pluripotent markers to detect contaminating residual undifferentiated pluripotent stem cells
Bem et al. Transient microRNA expression enhances myogenic potential of mouse embryonic stem cells
US20200216813A1 (en) Cell reprogramming to cardiomyocytes
CN119365606A (zh) 未分化多能干细胞的检测方法和检测试剂
Hiramoto et al. Non-transmissible MV vector with segmented RNA genome establishes different types of iPSCs from hematopoietic cells
Harkness et al. Molecular characterisation of stromal populations derived from human embryonic stem cells: Similarities to immortalised bone marrow derived stromal stem cells
WO2023118050A1 (en) Use of novel markers to detect pluripotent stem cells
EP3950932B1 (en) Method for producing pluripotent stem cells capable of differentiating into specific cells, and application thereof

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination