CN119173628A - 通过低pH处理从RNA制备物中除去双链和/或多链核酸杂质的方法 - Google Patents

通过低pH处理从RNA制备物中除去双链和/或多链核酸杂质的方法 Download PDF

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Publication number
CN119173628A
CN119173628A CN202380029206.4A CN202380029206A CN119173628A CN 119173628 A CN119173628 A CN 119173628A CN 202380029206 A CN202380029206 A CN 202380029206A CN 119173628 A CN119173628 A CN 119173628A
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CN
China
Prior art keywords
rna
chromatography
stranded
range
mrna
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Pending
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CN202380029206.4A
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English (en)
Chinese (zh)
Inventor
亚斯米娜·普奇
波罗纳·梅古萨
罗克·塞基尔尼克
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Sedolis Biya Separation Co ltd
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Sedolis Biya Separation Co ltd
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Application filed by Sedolis Biya Separation Co ltd filed Critical Sedolis Biya Separation Co ltd
Publication of CN119173628A publication Critical patent/CN119173628A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/02—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
CN202380029206.4A 2022-04-07 2023-04-06 通过低pH处理从RNA制备物中除去双链和/或多链核酸杂质的方法 Pending CN119173628A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP22167222.3A EP4257685A1 (en) 2022-04-07 2022-04-07 Methods for the removal of double- and/or multi-stranded nucleic acid impurities from rna preparations by low ph treatment
EP22167222.3 2022-04-07
PCT/EP2023/059215 WO2023194562A1 (en) 2022-04-07 2023-04-06 METHODS FOR THE REMOVAL OF DOUBLE- AND/OR MULTI-STRANDED NUCLEIC ACID IMPURITIES FROM RNA PREPARATIONS BY LOW pH TREATMENT

Publications (1)

Publication Number Publication Date
CN119173628A true CN119173628A (zh) 2024-12-20

Family

ID=81846517

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202380029206.4A Pending CN119173628A (zh) 2022-04-07 2023-04-06 通过低pH处理从RNA制备物中除去双链和/或多链核酸杂质的方法

Country Status (6)

Country Link
US (1) US20250207121A1 (https=)
EP (1) EP4257685A1 (https=)
JP (1) JP2025511324A (https=)
KR (1) KR20240153398A (https=)
CN (1) CN119173628A (https=)
WO (1) WO2023194562A1 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117603958B (zh) * 2023-11-23 2024-07-16 江苏耀海生物制药有限公司 一种纯化体外转录mRNA的方法及应用

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6120985A (en) * 1997-10-31 2000-09-19 Bbi Bioseq, Inc. Pressure-enhanced extraction and purification
US7794932B2 (en) * 2004-04-16 2010-09-14 Piotr Chomczynski Reagents and methods for isolation of purified RNA
EP1963526A4 (en) * 2005-12-09 2009-11-18 Promega Corp PURIFICATION OF NUCLEIC ACID WITH A BINDING MATRIX
US10954507B2 (en) * 2014-07-17 2021-03-23 Qiagen Gmbh Method for isolating RNA with high yield

Also Published As

Publication number Publication date
EP4257685A1 (en) 2023-10-11
US20250207121A1 (en) 2025-06-26
WO2023194562A1 (en) 2023-10-12
JP2025511324A (ja) 2025-04-15
KR20240153398A (ko) 2024-10-22

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