CN1191468C - Colourimetric fast determination tube for nitrite in water - Google Patents
Colourimetric fast determination tube for nitrite in water Download PDFInfo
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- CN1191468C CN1191468C CN 02115551 CN02115551A CN1191468C CN 1191468 C CN1191468 C CN 1191468C CN 02115551 CN02115551 CN 02115551 CN 02115551 A CN02115551 A CN 02115551A CN 1191468 C CN1191468 C CN 1191468C
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Abstract
The present invention relates to a measuring device for nitrite in water, particularly to a colorimetric fast measuring tube for nitrite in water. The present invention comprises a vacuum glass tube with a constant section and nitrite measuring liquid, wherein the nitrite measuring liquid contains 10% of quinonimine dye, 40% of coordination acid and solvent. Simultaneously, the ratio of the volumes of the quinonimine dye, the coordination acid and the solvent is (0.5 to 1.5): (2 to 4): (1 to 4). The ratio formula between the height H1 of the measuring liquid and the height H2 of a left blank of the constant section is H1: H2=1: (3 to 6). The use level of the quinonimine dye and the coordination acid is relatively reduced or enlarged as the high and low variation of the concentration of the quinonimine dye and the coordination acid. The measuring liquid in the measuring tube is non-toxic, harmless and safe, and the present invention can fast, accurately and reliably provide the measuring data. Moreover, the present invention has wide application so that the present invention can be used in the site of the environmental protection, the aquaculture and the sewage disposal. Furthermore, the present invention has the important operation significance of the water quality on-site monitoring operation, and the present invention has better economic and social benefits.
Description
(1) technical field: the present invention relates to a kind of utensil of measuring nitrite, particularly relate to the mensuration utensil of nitrite in a kind of water quality.
(2) background technology: detect in the water quality whether have the nitrite material, its significant meaning is arranged.If the nitrite in the water quality enters in the blood of human body circulation, can make the normal haemoglobin of human body (low Ferri-hemoglobin) be oxidized to methemoglobin, make haemoglobin lose the ability of delivering oxygen in vivo, cause tissue the symptom of anoxic to occur.In addition, nitrite can with secondary amine class substance reaction, generate and to have carcinogenicity nitrous acid amine material, so detect the nitrite in the water quality, have special meaning and value.Another characteristic of nitrite is subject to microbial action and very unstable in water, based on this characteristic, the detection of content of nitrite should be carried out at the scene in the water quality, promptly with water sampling, tests immediately at any time, could detect the truth of on-the-spot water sample like this.Nitrite in the traditional detection water quality generally adopts the spectrophotometric method or the chromatography of ions, must use instrument and equipment and professional's test, traditional detection method can't be realized on-the-spot test, has only water sampling at the scene, preserve water sample at 4 ℃ of environment, fetching the laboratory should test immediately, otherwise the data of surveying can't be represented its authenticity.
Adopt Spectrophotometric Determination of Nitrite, spectrophotometric method adopts diazonium-coupling reaction usually, employed coupling reagent generally is alpha-naphthylamine and N-(1 naphthyl)-ethylenediamine, all is strong carcinogen, might enter human body by skin contact or respiratory tract.And this type of reagent instability was generally only preserved one month under 2～5 ℃ of conditions, and the testing staff will often contact this material inevitably.
Its operation steps, (one), reagent preparation: 1. phosphoric acid, 2. diazo reagent, 3. coupling reagent, 4. diazonium-coupling reagent, 5. nitrite standard solution, 6. liquor potassic permanganate, 7. sodium oxalate standard solution.(2), demarcate the nitrite standard solution, (using titrimetry), (3), make working curve, prepare the nitrite standard of a series of variable concentrations and use liquid, adding respectively after diazonium-coupling reagent leaves standstill 20 minutes, measure corresponding absorbance with spectrophotometric, is horizontal ordinate with the concentration value, absorbance is an ordinate, makes working curve.(4), the test water sample, regulate the water sample pH value with phosphoric acid, add diazonium-coupling reagent, then by the same steps as of making working curve, measure the absorbance of water sample, find the nitrite concentration value of water sample from working curve.The used reagent of Spectrophotometric Determination of Nitrite is many, and it is numerous and diverse, time-consuming to work, testing staff's contact be that some are strong carcinogens, be harmful to health.
(3) summary of the invention:
The objective of the invention is: adopt new method to measure nitrite, make nitrite colorimetric cylinder in the water quality.
The technical solution used in the present invention is:
Nitrite colorimetric cylinder in a kind of water quality, contain the uniform cross section glass tube with vacuum, uniform cross section glass tube with vacuum one end has one section communication seals kapillary, nitrite is measured liquid, nitrite is measured liquid and is contained 10% quinoneimine dye, 40% coordination acid, solvent. the ratio of three's volume is 0.5～1.5: 2～4: 1～4, measure the height H of liquid in measuring pipe
1With measure in the pipe uniform cross section height H of leaving a blank
2, H
1: H
2=1: 3～6, the concentration of quinoneimine dye and coordination acid improves or reduces its volume and can correspondingly dwindle or enlarge, and coordination acid is a phosphoric acid, and solvent is an ethanol, or acetone, or distilled water, or deionized water, or any solvent in the molecule membrane filtration water.
Nitrite colorimetric cylinder in a kind of water quality, contain the uniform cross section glass tube with vacuum, uniform cross section glass tube with vacuum one end has one section communication seals kapillary, nitrite is measured liquid, and nitrite is measured liquid and contained 1% quinoneimine dye, 40% coordination acid, solvent, the ratio of three's volume is: 1～5: 5～10: 5～15, and the concentration of quinoneimine dye and coordination acid improves or reduces its volume and can correspondingly dwindle or enlarge, and measures the height H of liquid in measuring pipe
1With measure in the pipe uniform cross section height H of leaving a blank
2, H
1: H
2=1: 3～6,40% coordination acid is the acid mixture that 20% sulfuric acid adds 20% phosphoric acid, and solvent is an ethanol, or acetone, or distilled water, or deionized water, or any solvent in the molecule membrane filtration water.
Useful good effect of the present invention:
(1) kapillary that fractures in water sample, water sample charge into to measure automatically measures the liquid reaction solution in the Guan Bingyu pipe, treated in about 2 minutes behind the colour stable directly and the contrast of standard colour code, can read nitrite concentration value in the water sample, assay method novelty, quick.
(2) measure liquid sealing in measuring pipe, needn't repeat preparation, with the measuring technology commercialization, test products is for measuring pipe.Simplify determination step greatly, reduce labour intensity greatly, shorten the test duration greatly.
(3) mensuration liquid reagent itself is nontoxic, does not have pollution, and is healthy harmless to the testing staff.
(4) chromogenic reaction of mensuration liquid and determinand nitrite is to finish in measuring pipe, cuts down the number of intermediate links, and noiseless, testing result accurately and reliably.
(5) another kind of characteristics of the present invention are: implement to detect the Other Instruments equipment that do not need, not electricity consumption, easily popularize, be specially adapted to on-the-spot the detection, the present invention can be widely used in potable water, surface water, underground water, sanitary sewage, industrial waste water, aquaculture, the mensuration of nitrite in the water quality such as soilless culture water.On-site supervision has important practical usage to the present technique product for water quality.
(4) description of drawings:
Fig. 1 is that nitrite is measured the tubular construction synoptic diagram in the water quality.
(5) embodiment: implement nitrite colorimetric fast measuring pipe in the water quality, at first to make a series of nitrite different contents, or title variable concentrations standard charomatron, form a series of standard color range color development system, and color fixed, make it colour-fast, charomatron makes its color accurate by spectrophotometer adjustment and demarcation.
Embodiment one, referring to Fig. 1,1 is uniform cross section glass tube with vacuum body among the figure, and 2 are the sealing capillary tube segment, and 3 for measuring liquid, get 0.5ml 10% quinoneimine dye, 2ml 40% coordination acid phosphoric acid, the 1ml etoh solvent is made into and measures liquid 3.5ml, measure pipe uniform cross section overall diameter 7.5mm, overall height is 10cm, and kapillary height 2.0cm measures the liquid height H
1With the height H of leaving a blank
2Ratio got H 1: 3
1Be 2.0cm, H
2Be 6cm.
Embodiment two, referring to Fig. 1, each the numbering represent meaning with embodiment one, do not repeat.Get 1ml 10% quinoneimine dye, 40% coordination acid, i.e. 20% sulfuric acid, 20% phosphoric acid acid mixture 3ml, solvent acetone 2.5ml is made into 6.5ml and measures liquid, measures the pipe physical dimension, with embodiment one, H
1: H
2=1: 4, get H
1=1.6 (cm), H
2=6.4 (cm).
Embodiment three, referring to Fig. 1, its structure and physical dimension thereof do not repeat with embodiment one.Get 1.5ml 10% quinoneimine dye, 40% coordination acid phosphatase 24 ml, etoh solvent 4ml is made into 9.5ml and measures liquid, H
1: H
2Got 1: 5, and got H
1=1.33 (cm), H
2=6.67 (cm).
Embodiment four: referring to Fig. 1, its structure and physical dimension thereof do not repeat with embodiment one.Get 1% quinoneimine dye 1ml, 20% sulfuric acid 2.5ml, 20% phosphoric acid 2.5ml, solvent is deionized water 5ml, is made into 11ml and measures liquid, gets H
1: H
2=1: 4cm, H
1Be 1.6cm, H
2Be 6.4cm.
Claims (2)
1. nitrite colorimetric cylinder in the water quality, contain the uniform cross section glass tube with vacuum, uniform cross section glass tube with vacuum one end has one section communication seals kapillary, nitrite is measured liquid, it is characterized in that: nitrite is measured liquid and is contained 10% quinoneimine dye, 40% coordination acid, solvent, the ratio of three's volume is 0.5～1.5: 2～4: 1～4, measure the height H of liquid in measuring pipe
1With measure in the pipe uniform cross section height H of leaving a blank
2, H
1: H
2=1: 3～6, the concentration of quinoneimine dye and coordination acid improves or reduces its volume and can correspondingly dwindle or enlarge, and coordination acid is a phosphoric acid, and solvent is an ethanol, or acetone, or distilled water, or deionized water, or any solvent in the molecule membrane filtration water.
2. nitrite colorimetric cylinder in the water quality, contain the uniform cross section glass tube with vacuum, uniform cross section glass tube with vacuum one end has one section communication seals kapillary, nitrite is measured liquid, it is characterized in that: nitrite is measured liquid and is contained 1% quinoneimine dye, 40% coordination acid, solvent, the ratio of three's volume is: 1～5: 5～10: 5～15, and the concentration of quinoneimine dye and coordination acid improves or reduces its volume and can correspondingly dwindle or enlarge, and measures the height H of liquid in measuring pipe
1With measure in the pipe uniform cross section height H of leaving a blank
2, H
1: H
2=1: 3～6,40% coordination acid is the acid mixture that 20% sulfuric acid adds 20% phosphoric acid, and solvent is an ethanol, or acetone, or distilled water, or deionized water, or any solvent in the molecule membrane filtration water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02115551 CN1191468C (en) | 2002-02-08 | 2002-02-08 | Colourimetric fast determination tube for nitrite in water |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02115551 CN1191468C (en) | 2002-02-08 | 2002-02-08 | Colourimetric fast determination tube for nitrite in water |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1414376A CN1414376A (en) | 2003-04-30 |
| CN1191468C true CN1191468C (en) | 2005-03-02 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 02115551 Expired - Fee Related CN1191468C (en) | 2002-02-08 | 2002-02-08 | Colourimetric fast determination tube for nitrite in water |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1766563B (en) * | 2005-11-09 | 2011-11-23 | 白莉 | Molybdenum determination solution and colorimetric determination tube therefor |
| CN100510708C (en) * | 2005-11-09 | 2009-07-08 | 白莉 | Aluminium determination solution and colorimetric determination tube therefor |
| CN106596527B (en) * | 2016-10-13 | 2019-11-12 | 温州大学 | A kind of organic/inorganic composite and its preparation and nitrite ion detection with separate in application |
| CN108732299B (en) * | 2018-06-01 | 2020-10-16 | 遵义医科大学 | Colorimetric detection method for nitrite in water |
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2002
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Granted publication date: 20050302 Termination date: 20140208 |