CN1975380A - Reagent formula of optical detection for assaying human body hemoglobin - Google Patents
Reagent formula of optical detection for assaying human body hemoglobin Download PDFInfo
- Publication number
- CN1975380A CN1975380A CN 200510126008 CN200510126008A CN1975380A CN 1975380 A CN1975380 A CN 1975380A CN 200510126008 CN200510126008 CN 200510126008 CN 200510126008 A CN200510126008 A CN 200510126008A CN 1975380 A CN1975380 A CN 1975380A
- Authority
- CN
- China
- Prior art keywords
- reagent
- optical detection
- acid
- sodium
- formula
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
This invention disclosed a reagent formulation for haemoglobin testing in human body. It contains at least an oxidation-reduction reaction reagent, a decomposition reagent, a diffusant and at least a stabilizing agent. It is very safe to handle and it works effectively and precisely. Additionally, the testing range was wide and the cost was low.
Description
Technical field
The present invention relates to detectable, belong to the medical clinic applications technical field, is a kind of reagent formula of optical detection of measuring human body hemoglobin.
Background technology
Haemoglobin is erythroblast and the synthetic a kind of conjugated protein that the pigment prothetic group is arranged of granulophilocyte in the body, and the main effect in human body is the transportation that participates in oxygen.
Hypoferric anemia is the nutrition problem of common harm population health.The mensuration of content of hemoglobin is one of basic skills of evaluation crowd anaemia morbidity rate and iron nutrition condition.Its assay method is recommended with the standardization council of International Society for the Study of Behavioural Development (ICSH), is the method for standard through cyanmethemoglobin (HiCN) spectrophotometry of The World Health Organization's approval.But, owing to contain poisonous drugs potassium cyanide (KCN) in the cyanmethemoglobin reagent, though consumption is few in this reagent, less than the human body lethal quantity, if but a large amount of long-term this reagent that uses adds that liquid waste processing is improper, will certainly cause environmental pollution or harmful to human, need special messenger's keeping, use inconvenience; When cyanmethemoglobin reagent was measured, haemoglobin needs the regular hour to be converted into cyanmethemoglobin (need approximately 5min after colorimetric estimation), and was consuming time longer, and necessary instrument is huge, and not portable, cost is higher, is difficult to penetration and promotion.
Summary of the invention
Purpose of the present invention, it is exactly deficiency at above-mentioned method of testing, to clinical provide a kind of do not contain potassium cyanide (KCN), cheap, easy preservation, can rapid reaction, color stability, disturbing factor be little, be better than cyanmethemoglobin (HiCN) method and with conventional method the test agent prescription of high correlation is arranged, thereby set up a kind of simple and efficient hemoglobin level in-situ check and test method, can solve a large amount of public health works on the spot.
A further object of the present invention provides a kind of test agent prescription, and this reagent can be used for the haemoglobin in the clinical medicine quantitative measurement blood; Can carry out field quick detection, satisfy the needs of medicine body and can effectively eliminate cross-infection between individuality.
For reaching above purpose, technical solution of the present invention provides a kind of reagent formula of optical detection of measuring human body hemoglobin, it comprises at least a redox reaction reagent and at least a many carbon decomposition agent, and after these two kinds of reagent are carried out ratio optimization, at least a organic diffusant and at least a inorganic stabilizers have been added.
Described reagent formula of optical detection, its described redox reaction reagent is to close cobalt acid potassium, the biscyclo pentadienyl iron from yellow natrojarosite, ferric trichloride, iron sulfate, Hexacyanoferrate potassium, ferric nitrate, potassium hexacyanocobaltate, chlorination six cobaltammines, six nitrous acid to choose a kind of composition at least.
Described reagent formula of optical detection, the redox reaction reagent that it is selected, its concentration is preferably 9g/L between 8.0～12.0g/L.
Described reagent formula of optical detection, its described many carbon decomposition agent, be from dodecyl creatine sodium, Triton X-100, glacial acetic acid, saponin, phenyl-4,5-dimethylthiazole chlorine drone salt, sodium dodecyl sulfate polyacrylamide, the N-acetylamino grape pool, 3-[2-(4-methoxyl-phenyl)-2-oxygen-ethyl]-4,5,6,7-tetrahydrochysene-benzothiazole bromide, 3-[2-(4-nitro-phenyl)-2-oxygen-ethyl]-4,5,6,7-tetrahydrochysene-benzothiazole bromide, 3-2-naphthalene-2-base-2-oxygen-ethyl)-4,5,6, choose a kind of composition at least in the 7-tetrahydrochysene-benzothiazole bromide.
Described reagent formula of optical detection, its selected many carbon decomposition agent, its concentration is preferably 13g/L between 10～14g/L.
Described reagent formula of optical detection, its described organic diffusant is to choose a kind of composition at least from sodium metnylene bis-naphthalene sulfonate, monolaurin, polyglycerol fatty acid, polyoxyethylenealkylphenol ether, 18 phosphoamides, polyoxyethylene (4) sorbitol anhydride monolaurate, Qu Latong, ethylene glycol monobutyl ether (EGMBE), aliphatic acid polyethenoxy ether, dodecyl tertiary ammonium salt, 4-vinylpridine, coconut acid diethanolamine condensation product, maleic acid di-sec-octyl sodium sulfonate, dodecyl sulphate diethanolamine salt, sodium p-octadecyl toluene sulfonate, oily sodium sulfonate.
Described reagent formula of optical detection, organic diffusant that it is selected, its concentration is preferably 0.50g/L between 0.10～0.50g/L.
Described reagent formula of optical detection, its described inorganic stabilizers is from gel, arginine, oxalic acid, oxine, EDTA, ammoniacal liquor-sal-ammoniac, Ca (H
2PO
4)
2-CaHPO
4, boric acid, phosphoric acid, acetate, NaOH, Na
2CO
3-NaHCO
3, NaH
2PO
4-Na
2HPO
4, choose a kind of composition at least in the sodium acetate, NaTDC, barium chloride, triethanolamine, sucrose phosphoric acid, glycocoll, sodium acetate.
Described reagent formula of optical detection, the inorganic stabilizers that it is selected, its concentration is preferably 5.0g/L between 2.0～6.0g/L.
Described reagent formula of optical detection, the inorganic stabilizers that it is selected can be regulated the pH value of this optical detection with reagent, and its pH value is between 6.5～7.5.
Description of drawings
Fig. 1 utilizes agent prescription of the present invention to detect the correlativity test synoptic diagram of haemoglobin and ADVIA 120 Biochemical Analyzers.
Embodiment
The present invention proposes a kind of reagent formula of optical detection of measuring human body hemoglobin, this prescription comprises following four kinds of reagent at least:
At least a redox reaction reagent, described redox reaction reagent closes cobalt acid potassium, the biscyclo pentadienyl iron from yellow natrojarosite, ferric trichloride, iron sulfate, Hexacyanoferrate potassium, ferric nitrate, potassium hexacyanocobaltate, chlorination six cobaltammines, six nitrous acid and chooses, used concentration is chosen at 8.0～12.0g/L, is preferably 9g/L.
This reagent and haemoglobin reaction can make final product generation change color, and haemoglobin is proportional in shade and the blood, utilize the reflection of light principle of absorption to get final product haemoglobin in the detection by quantitative blood.
At least a many carbon decomposition agent, described many carbon decomposition agent is from dodecyl creatine sodium, Triton X-100, glacial acetic acid, saponin, phenyl-4,5-dimethylthiazole chlorine drone salt, sodium dodecyl sulfate polyacrylamide, the N-acetylamino grape pool, 3-[2-(4-methoxyl-phenyl)-2-oxygen-ethyl]-4,5,6,7-tetrahydrochysene-benzothiazole bromide, 3-[2-(4-nitro-phenyl)-2-oxygen-ethyl]-4,5,6,7-tetrahydrochysene-benzothiazole bromide, 3-2-naphthalene-2-base-2-oxygen-ethyl)-4,5,6, choose in the 7-tetrahydrochysene-benzothiazole bromide, concentration is got fixed between 10～14g/L, is preferably 13g/L.
The existence of this reagent can be carried out cracking to the red blood cell in the blood, thereby discharges haemoglobin.
At least a organic diffusant, described organic diffusant is from sodium metnylene bis-naphthalene sulfonate, monolaurin, polyglycerol fatty acid, polyoxyethylenealkylphenol ether, 18 phosphoamides, polyoxyethylene (4) sorbitol anhydride monolaurate, Qu Latong, ethylene glycol monobutyl ether (EGMBE), aliphatic acid polyethenoxy ether, the dodecyl tertiary ammonium salt, 4-vinylpridine, coconut acid diethanolamine condensation product, maleic acid di-sec-octyl sodium sulfonate, the dodecyl sulphate diethanolamine salt, sodium p-octadecyl toluene sulfonate, choose in the oil sodium sulfonate, concentration is got fixed between 0.10～0.50g/L, is preferably 0.50g/L.
Selected inorganic diffusant can make the sample that drips scatter fast, fully contacts with reaction reagent, shortens the reaction time.
At least a inorganic stabilizers, described inorganic stabilizers is from gel, arginine, oxalic acid, oxine, EDTA, Tris-HCL, ammoniacal liquor-sal-ammoniac, Ca (H
2PO
4)
2-CaHPO
4, boric acid, phosphoric acid, acetate, NaOH, Na
2CO
3-NaHCO
3, NaH
2PO
4-Na
2HPO
4, choose in the sodium acetate, NaTDC, barium chloride, triethanolamine, sucrose phosphoric acid, glycocoll, sodium acetate, used concentration is got fixed between 2.0～6.0g/L, is preferably 5.0g/L.
Used inorganic stabilizers can increase the stability of the haemoglobin molecule that discharges from red blood cell, make color stability, and this inorganic stabilizers regulates the function of reagent pH in addition, regulates reagent pH value between 6.5～7.5.
Present invention is described below in conjunction with specific embodiment:
Embodiment 1
With prepared at concentrations shown in the following compounds a kind of reagent:
Compound concentration
Hexacyanoferrate potassium 9g/L
Saponin 13g/L
NaH
2PO
4-Na
2HPO
4 5.0g/L
Triton x-100 0.50g/L
With above reagent by shown in concentration mix, and regulate pH between 6.5～7.5, thereby prepare a kind of haemoglobin test agent.Utilize this reagent, by optical detection, be the contrast experiment at Beijing Hospital's clinical examination quarantine centers and ADVIA 120 bio-chemical detectors, response time 60s, comparing result as shown in Figure 1, two kinds of reagent tests related coefficient as a result reach 0.986, have obtained quite good detecting effectiveness, can satisfy the needs of field quick detection.
Above embodiment is just in order to illustrate the present invention, rather than limitation of the present invention.On the basis of the above description, the present invention can be many modifications and changes.Within the scope of the appended claims, the present invention can have and is different from other above-mentioned implementation, selects other reagent for use, changes methods such as reagent concentration all within patent claimed range of the present invention.
Claims (10)
1. reagent formula of optical detection of measuring human body hemoglobin, it is characterized in that, comprise at least a redox reaction reagent and at least a many carbon decomposition agent, and after these two kinds of reagent are carried out ratio optimization, added at least a organic diffusant and at least a inorganic stabilizers.
2. reagent formula of optical detection as claimed in claim 1, it is characterized in that, described redox reaction reagent is to close cobalt acid potassium, the biscyclo pentadienyl iron from yellow natrojarosite, ferric trichloride, iron sulfate, Hexacyanoferrate potassium, ferric nitrate, potassium hexacyanocobaltate, chlorination six cobaltammines, six nitrous acid to choose a kind of composition at least.
3. reagent formula of optical detection as claimed in claim 2 is characterized in that, selected redox reaction reagent, and its concentration is preferably 9g/L between 8.0～12.0g/L.
4. reagent formula of optical detection as claimed in claim 1, it is characterized in that, described many carbon decomposition agent, be from dodecyl creatine sodium, Triton X-100, glacial acetic acid, saponin, phenyl-4,5-dimethylthiazole chlorine drone salt, sodium dodecyl sulfate polyacrylamide, the N-acetylamino grape pool, 3-[2-(4-methoxyl-phenyl)-2-oxygen-ethyl]-4,5,6,7-tetrahydrochysene-benzothiazole bromide, 3-[2-(4-nitro-phenyl)-2-oxygen-ethyl]-4,5,6,7-tetrahydrochysene-benzothiazole bromide, 3-2-naphthalene-2-base-2-oxygen-ethyl)-4,5,6, choose a kind of composition at least in the 7-tetrahydrochysene-benzothiazole bromide.
5. reagent formula of optical detection as claimed in claim 4 is characterized in that, selected many carbon decomposition agent, and its concentration is preferably 13g/L between 10～14g/L.
6. reagent formula of optical detection as claimed in claim 1, it is characterized in that, described organic diffusant is from sodium metnylene bis-naphthalene sulfonate, monolaurin, polyglycerol fatty acid, polyoxyethylenealkylphenol ether, 18 phosphoamides, polyoxyethylene (4) sorbitol anhydride monolaurate, Qu Latong, ethylene glycol monobutyl ether (EGMBE), aliphatic acid polyethenoxy ether, the dodecyl tertiary ammonium salt, 4-vinylpridine, coconut acid diethanolamine condensation product, maleic acid di-sec-octyl sodium sulfonate, the dodecyl sulphate diethanolamine salt, sodium p-octadecyl toluene sulfonate, at least choose a kind of composition in the oil sodium sulfonate.
7. reagent formula of optical detection as claimed in claim 6 is characterized in that, selected organic diffusant, and its concentration is preferably 0.50g/L between 0.10～0.50g/L.
8. reagent formula of optical detection as claimed in claim 1 is characterized in that, described inorganic stabilizers is from gel, arginine, oxalic acid, oxine, EDTA, ammoniacal liquor-sal-ammoniac, Ca (H
2PO
4)
2-CaHPO
4, boric acid, phosphoric acid, acetate, NaOH, Na
2CO
3-NaHCO
3, NaH
2PO
4-Na
2HPO
4, choose a kind of composition at least in the sodium acetate, NaTDC, barium chloride, triethanolamine, sucrose phosphoric acid, glycocoll, sodium acetate.
9. reagent formula of optical detection as claimed in claim 8 is characterized in that, selected inorganic stabilizers, and its concentration is preferably 5.0g/L between 2.0～6.0g/L.
10. as claim 1,8 or 9 described reagent formula of optical detection, it is characterized in that selected inorganic stabilizers can be regulated the pH value of this optical detection with reagent, its pH value is between 6.5～7.5.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510126008 CN1975380A (en) | 2005-11-28 | 2005-11-28 | Reagent formula of optical detection for assaying human body hemoglobin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510126008 CN1975380A (en) | 2005-11-28 | 2005-11-28 | Reagent formula of optical detection for assaying human body hemoglobin |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1975380A true CN1975380A (en) | 2007-06-06 |
Family
ID=38125603
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200510126008 Pending CN1975380A (en) | 2005-11-28 | 2005-11-28 | Reagent formula of optical detection for assaying human body hemoglobin |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1975380A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101828112A (en) * | 2007-10-16 | 2010-09-08 | 荣研化学株式会社 | Method of stabilizing hem protein and storage solution therefor |
| CN102300641A (en) * | 2009-01-27 | 2011-12-28 | 皇家飞利浦电子股份有限公司 | Microfluidic device for full blood count |
| CN103913454A (en) * | 2014-04-04 | 2014-07-09 | 重庆医科大学 | Preparation method of color chart for rapidly detecting content of methemoglobin |
| CN106706891A (en) * | 2017-01-03 | 2017-05-24 | 长沙中生众捷生物技术有限公司 | Hemoglobin detection reagent and hemoglobin detection paper |
-
2005
- 2005-11-28 CN CN 200510126008 patent/CN1975380A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101828112A (en) * | 2007-10-16 | 2010-09-08 | 荣研化学株式会社 | Method of stabilizing hem protein and storage solution therefor |
| CN101828112B (en) * | 2007-10-16 | 2013-11-13 | 荣研化学株式会社 | Method of stabilizing hem protein and storage solution therefor |
| CN102300641A (en) * | 2009-01-27 | 2011-12-28 | 皇家飞利浦电子股份有限公司 | Microfluidic device for full blood count |
| US10191054B2 (en) | 2009-01-27 | 2019-01-29 | Koninklijke Philips N.V. | Microfluidic device for full blood count |
| CN103913454A (en) * | 2014-04-04 | 2014-07-09 | 重庆医科大学 | Preparation method of color chart for rapidly detecting content of methemoglobin |
| CN103913454B (en) * | 2014-04-04 | 2017-01-25 | 重庆医科大学 | Preparation method of color chart for rapidly detecting content of methemoglobin |
| CN106706891A (en) * | 2017-01-03 | 2017-05-24 | 长沙中生众捷生物技术有限公司 | Hemoglobin detection reagent and hemoglobin detection paper |
| CN106706891B (en) * | 2017-01-03 | 2019-03-01 | 长沙中生众捷生物技术有限公司 | The detection reagent of hemoglobin and the Test paper of hemoglobin |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Jones et al. | Standard dilution analysis | |
| Erbao et al. | Flow injection determination of adenine at trace level based on luminol–K2Cr2O7 chemiluminescence in a micellar medium | |
| CA2322858A1 (en) | Detection of analytes by fluorescent lanthanide chelates | |
| CN103411943B (en) | Method for detecting Ag<+1>, Hg<2+> and Fe<3+> based on fluorescence quenching method or colorimetric method of erythrosine B | |
| WO2012016350A1 (en) | Simultaneous determination of multiple analytes in industrial water system | |
| CN1975380A (en) | Reagent formula of optical detection for assaying human body hemoglobin | |
| Freeman et al. | Flow-injection technique for the determination of low levels of phosphorus in natural waters | |
| Kojło et al. | Chemiluminescence determination of thioridazine hydrochloride by flow-injection analysis | |
| Lian et al. | A study on terbium sensitized chemiluminescence of ciprofloxacin and its application | |
| Molina-Dıaz et al. | Indirect spectrophotometric determination of ascorbic acid with ferrozine by flow-injection analysis | |
| Weltje et al. | Lutetium speciation and toxicity in a microbial bioassay: Testing the free-ion model for lanthanides | |
| Wang et al. | A selective optical chemical sensor for o-nitrophenol based on fluorescence quenching of curcumin | |
| Yusof et al. | A flow cell optosensor for lead based on immobilized gallocynin in chitosan membrane | |
| Chromý et al. | Spectrophotometric determination of magnesium in biological fluids with xylidyl blue II | |
| US20030138959A1 (en) | Method of detecting oxidizing adulterants in urine | |
| CN1815201A (en) | Visual colorimetric fluorine ion sensing test-paper and use thereof | |
| McLamore et al. | Development and validation of an open source O2-sensitive gel for physiological profiling of soil microbial communities | |
| Ioannou et al. | Simple, rapid and sensitive spectrofluorimetric determination of diflunisal in serum and urine based on its ternary complex with terbium and EDTA | |
| Gutierrez et al. | Individual and simultaneous stopped-flow fluorimetric determination of perphenazine and chlorpromazine | |
| Jin et al. | Room temperature phosphorescence pH optosensor based on energy transfer | |
| Reddy et al. | A New Spectrophotometric Determination of Famotidine from Tablets. | |
| CN1281983A (en) | Serum tolal bile acid determination reagent | |
| CN107121420A (en) | One kind detects S based on calcein rhodamine B gold ion system2‑Method | |
| Nikolov et al. | Virtual colorimeter for water analysis | |
| Alava-Moreno et al. | Kalman filtering-aided time-resolved solid-surface room temperature phosphorimetry for simultaneous determination of tetracyclines in solution |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |