CN1414376A - Colourimetric fast determination tube for nitrite in water - Google Patents

Colourimetric fast determination tube for nitrite in water Download PDF

Info

Publication number
CN1414376A
CN1414376A CN 02115551 CN02115551A CN1414376A CN 1414376 A CN1414376 A CN 1414376A CN 02115551 CN02115551 CN 02115551 CN 02115551 A CN02115551 A CN 02115551A CN 1414376 A CN1414376 A CN 1414376A
Authority
CN
China
Prior art keywords
nitrite
acid
water
coordination
solvent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN 02115551
Other languages
Chinese (zh)
Other versions
CN1191468C (en
Inventor
白莉
李树华
王静斌
权瑞
崔纪周
文德振
Original Assignee
白莉
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 白莉 filed Critical 白莉
Priority to CN 02115551 priority Critical patent/CN1191468C/en
Publication of CN1414376A publication Critical patent/CN1414376A/en
Application granted granted Critical
Publication of CN1191468C publication Critical patent/CN1191468C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Abstract

A colorimetric quick estimation tube for nitrite in water quality incldues a vacuum glass cylinder with constant section and a measuring liquid for nitrite composed of 10% quinonimine dye, 40%, coordination acid and solvent. The ratio of their volumes is (0.5-1.5):(2-4):(1-4). The rati oof between the height of the measurement liquid and the height of leaving a blank with constant sectino is H1:H2=1:(3-6). The use level is reduced or enlarged in proportion to the concentration of the quinonimine dye acid color and the coordination acid. The solvent of the measuring liquid is not poisonous, and is harmless and safe. The invention provides the fast, accurate and reliable data measured, and it can be utilized in the site of environmental protection, aquaculture and sewage disposal, providing good economic and social benefits.

Description

Nitrite colorimetric fast measuring pipe in the water quality
(1) technical field: the present invention relates to a kind of utensil of measuring nitrite, particularly relate to the mensuration utensil of nitrite in a kind of water quality.
(2) background technology: detect in the water quality whether have the nitrite material, its significant meaning is arranged.If the nitrite in the water quality enters in the blood of human body circulation, can make the normal haemoglobin of human body (low Ferri-hemoglobin) be oxidized to methemoglobin, make haemoglobin lose the ability of delivering oxygen in vivo, cause tissue the symptom of anoxic to occur.In addition, nitrite can with secondary amine class substance reaction, generate and to have carcinogenicity nitrous acid amine material, so detect the nitrite in the water quality, have special meaning and value.Another characteristic of nitrite is subject to microbial action and very unstable in water, based on this characteristic, the detection of content of nitrite should be carried out at the scene in the water quality, promptly with water sampling, tests immediately at any time, could detect the truth of on-the-spot water sample like this.Nitrite in the traditional detection water quality generally adopts the spectrophotometric method or the chromatography of ions, must use instrument and equipment and professional's test, traditional detection method can't be realized on-the-spot test, has only water sampling at the scene, preserve water sample at 4 ℃ of environment, fetching the laboratory should test immediately, otherwise the data of surveying can't be represented its authenticity.
Adopt Spectrophotometric Determination of Nitrite, spectrophotometric method adopts diazonium-coupling reaction usually, employed coupling reagent generally is alpha-naphthylamine and N-(1 naphthyl)-ethylenediamine, all is strong carcinogen, might enter human body by skin contact or respiratory tract.And this type of reagent instability was generally only preserved one month under 2~5 ℃ of conditions, and the testing staff will often contact this material inevitably.
Its operation steps, (one), reagent preparation: 1. phosphoric acid, 2. diazo reagent, 3. coupling reagent, 4. diazonium-coupling reagent, 5. nitrite standard solution, 6. liquor potassic permanganate, 7. sodium oxalate standard solution.(2), demarcate the nitrite standard solution, (using titrimetry), (3), make working curve, prepare the nitrite standard of a series of variable concentrations and use liquid, adding respectively after diazonium-coupling reagent leaves standstill 20 minutes, measure corresponding absorbance with spectrophotometric, is horizontal ordinate with the concentration value, absorbance is an ordinate, makes working curve.(4), the test water sample, regulate the water sample pH value with phosphoric acid, add diazonium-coupling reagent, then by the same steps as of making working curve, measure the absorbance of water sample, find the nitrite concentration value of water sample from working curve.The used reagent of Spectrophotometric Determination of Nitrite is many, and it is numerous and diverse, time-consuming to work, testing staff's contact be that some are strong carcinogens, be harmful to health.
(3) summary of the invention: the objective of the invention is: adopt new method to measure nitrite, make nitrite colorimetric fast measuring pipe in the water quality.
Technical scheme of the present invention is: nitrite colorimetric fast measuring pipe in a kind of water quality, contain the uniform cross section glass tube with vacuum, nitrite is measured liquid, nitrite is measured liquid and is contained 10% quinoneimine dye, 40% coordination acid, solvent, the ratio of three's volume is (0.5~1.5): (2~4): (1~4), measure liquid height H 1 and measure in the pipe uniform cross section height H of leaving a blank in measuring pipe 2, H 1: H 2=1: (3~6), the concentration of quinoneimine dye and coordination acid improves or reduces its volume and can correspondingly dwindle or enlarge.
Uniform cross section vacuum tube one end has the sealed hair tubule, and coordination acid can be phosphoric acid, and solvent can be ethanol, or is acetone or for distilled water, or is deionized water, or is molecule membrane filtration water.
Nitrite colorimetric fast measuring pipe in a kind of water quality, contain the uniform cross section glass tube with vacuum, nitrite is measured liquid, nitrite is measured liquid and is contained 1% quinoneimine dye, 40% coordination acid, solvent, three's ratio are example with the parts by volume: (1~5): (5~10): (5~15), quinoneimine dye and coordination acid concentration improve or reduce its volume and can correspondingly dwindle or enlarge, and measure the height H of liquid in measuring pipe 1Wait the height H of leaving a blank of cutting with measuring to manage 2, H 1: H 2Equal 1: (3~6).
Uniform cross section vacuum tube one end has the sealed hair tubule, and 40% coordination acid can be the acid mixture that 20% sulfuric acid adds 20% phosphoric acid, and solvent can be ethanol, or is acetone, or is distilled water, or is deionized water, or is molecule membrane filtration water.Useful good effect of the present invention:
(1) kapillary that in water sample, fractures, water sample charge into automatically testing tube and with pipe in measure the liquid reaction solution, treated in about 2 minutes behind the colour stable directly and the contrast of standard colour code, can read nitrite concentration value in the water sample, assay method novelty, quick.
(2) measure liquid sealing in measuring pipe, needn't repeat preparation, with the measuring technology commercialization, test products is for measuring pipe.Simplify determination step greatly, reduce labour intensity greatly, shorten the test duration greatly.
(3) mensuration liquid reagent itself is nontoxic, does not have pollution, and is healthy harmless to the testing staff.
(4) chromogenic reaction of mensuration liquid and determinand nitrite is to finish in measuring pipe, cuts down the number of intermediate links, and noiseless, testing result accurately and reliably.
(5) another kind of characteristics of the present invention are: implement to detect the Other Instruments equipment that do not need, not electricity consumption, easily popularize, be specially adapted to on-the-spot the detection, the present invention can be widely used in potable water, surface water, underground water, sanitary sewage, industrial waste water, aquaculture, the mensuration of nitrite in the water quality such as soilless culture water.On-site supervision has important practical usage to the present technique product for water quality.
(4) description of drawings:
Fig. 1 is that nitrite is measured the tubular construction synoptic diagram in the water quality.
(5) embodiment: implement nitrite colorimetric fast measuring pipe in the water quality, at first to make a series of nitrite different contents, or title variable concentrations standard charomatron, form a series of standard color range color development system, and color fixed, make it colour-fast, charomatron makes its color accurate by adjustment of beam split protractor and demarcation.
Embodiment one, referring to Fig. 1,1 is uniform cross section glass tube with vacuum body among the figure, and 2 are the sealing capillary tube segment, and 3 for measuring liquid, get 0.5ml 10% quinoneimine dye, 2ml 40% coordination acid phosphoric acid, the 1ml etoh solvent is made into and measures liquid 3.5ml, measure pipe uniform cross section overall diameter 7.5mm, overall height is 10cm, and kapillary height 2.0cm measures the liquid height H 1With the height H of leaving a blank 2Ratio got H 1: 3 1Be 2.0cm, H 2Be 6cm.
Embodiment two, referring to Fig. 1, each the numbering represent meaning with embodiment one, do not repeat.Get 1ml 10% quinoneimine dye, 40% coordination acid, i.e. 20% sulfuric acid, 20% phosphoric acid acid mixture 3ml, solvent acetone 2.5ml is made into 6.5ml and measures liquid, measures the pipe physical dimension, with embodiment one, H 1: H 2=1: 4, get H1=1.6 (cm), H 2=6.4 (cm).
Embodiment three, referring to Fig. 1, its structure and physical dimension thereof do not repeat with embodiment one.Get 1.5ml 10% quinoneimine dye, 4 0% coordination acid phosphatase 24 ml, etoh solvent 4ml is made into 9.5ml and measures liquid, H 1: H 2Got 1: 5, and got H 1=1.33 (cm), H 2=6.67 (cm).
Embodiment four: referring to Fig. 1, its structure and physical dimension thereof do not repeat with embodiment one.Get 1% quinoneimine dye 1ml, 20% sulfuric acid 2.5ml, 20% phosphoric acid 2.5ml, solvent is deionized water 5ml, is made into 11ml and measures liquid, gets H 1: H 2=1: 4cm, H 1Be 1.6cm, H 2Be 6.4cm.

Claims (4)

1. nitrite colorimetric fast measuring pipe in the water quality, contain the uniform cross section glass tube with vacuum, nitrite is measured liquid, it is characterized in that: nitrite is measured liquid and is contained 10% quinoneimine dye, 40% coordination acid, solvent, the ratio of three's volume is (0.5~1.5): (2~4): (1~4), measure liquid height H 1 and measure in the pipe uniform cross section height H of leaving a blank in measuring pipe 2, H 1: H 2=1: (3~6), the concentration of quinoneimine dye and coordination acid improves or reduces its volume and can correspondingly dwindle or enlarge.
2. mensuration pipe according to claim 1 is characterized in that: uniform cross section vacuum tube one end has the sealed hair tubule, and coordination acid can be phosphoric acid, and solvent can be ethanol, or is acetone or for distilled water, or is deionized water, or is molecule membrane filtration water.
3. nitrite colorimetric fast measuring pipe in the water quality, contain the uniform cross section glass tube with vacuum, nitrite is measured liquid, it waits to levy: nitrite is measured liquid and is contained 1% quinoneimine dye, 40% coordination acid, solvent, three's ratio are example with the parts by volume: (1~5): (5~10): (5~15), the height H of liquid in measuring pipe measured in the raising of quinoneimine dye and coordination acid concentration or reduce its volume and can correspondingly dwindle or enlarge 1Wait the height H of leaving a blank of cutting with measuring to manage 2, H 1: H 2Equal 1: (3~6).
4. mensuration pipe according to claim 3, it is characterized in that: uniform cross section vacuum tube one end has the sealed hair tubule, 40% coordination acid can be the acid mixture that 20% sulfuric acid adds 20% phosphoric acid, solvent can be ethanol, or be acetone, or be distilled water, or be deionized water, or be molecule membrane filtration water.
CN 02115551 2002-02-08 2002-02-08 Colourimetric fast determination tube for nitrite in water Expired - Fee Related CN1191468C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN 02115551 CN1191468C (en) 2002-02-08 2002-02-08 Colourimetric fast determination tube for nitrite in water

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN 02115551 CN1191468C (en) 2002-02-08 2002-02-08 Colourimetric fast determination tube for nitrite in water

Publications (2)

Publication Number Publication Date
CN1414376A true CN1414376A (en) 2003-04-30
CN1191468C CN1191468C (en) 2005-03-02

Family

ID=4743724

Family Applications (1)

Application Number Title Priority Date Filing Date
CN 02115551 Expired - Fee Related CN1191468C (en) 2002-02-08 2002-02-08 Colourimetric fast determination tube for nitrite in water

Country Status (1)

Country Link
CN (1) CN1191468C (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100510708C (en) * 2005-11-09 2009-07-08 白莉 Aluminium determination solution and colorimetric determination tube therefor
CN1766563B (en) * 2005-11-09 2011-11-23 白莉 Molybdenum determination solution and colorimetric determination tube therefor
CN106596527A (en) * 2016-10-13 2017-04-26 温州大学 Organic/inorganic compound as well as preparation thereof and applications to detection and separation of nitrite ions
CN108732299A (en) * 2018-06-01 2018-11-02 遵义医学院 The colorimetric detection method of water quality nitrite

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100510708C (en) * 2005-11-09 2009-07-08 白莉 Aluminium determination solution and colorimetric determination tube therefor
CN1766563B (en) * 2005-11-09 2011-11-23 白莉 Molybdenum determination solution and colorimetric determination tube therefor
CN106596527A (en) * 2016-10-13 2017-04-26 温州大学 Organic/inorganic compound as well as preparation thereof and applications to detection and separation of nitrite ions
CN106596527B (en) * 2016-10-13 2019-11-12 温州大学 A kind of organic/inorganic composite and its preparation and nitrite ion detection with separate in application
CN108732299A (en) * 2018-06-01 2018-11-02 遵义医学院 The colorimetric detection method of water quality nitrite
CN108732299B (en) * 2018-06-01 2020-10-16 遵义医科大学 Colorimetric detection method for nitrite in water

Also Published As

Publication number Publication date
CN1191468C (en) 2005-03-02

Similar Documents

Publication Publication Date Title
Udenfriend et al. Fluorescence characteristics of 5-hydroxytryptamine (serotonin)
CN103091262B (en) Miniaturized optical device for detecting ammonia nitrogen in water and detecting method
TW369599B (en) A reagent strip and a method for measuring glucose concentration in a sample of whole blood
Uttamlal et al. A fiber-optic carbon dioxide sensor for fermentation monitoring
CN101793902A (en) Device for fluidly injecting and rapidly analyzing residual chlorine of water quality and analysis method thereof
Mariotti et al. Determination of extra virgin olive oil acidity by FIA-titration
Revsbech et al. Amperometic microsensor for measurement of gaseous and dissolved CO2
CN1191468C (en) Colourimetric fast determination tube for nitrite in water
RU2235998C2 (en) Method of determination of oxidant/anti-oxidant activity of solutions
Jain et al. Analysis of alcohol I. A review of chemical and infrared methods
CN2560944Y (en) Rapid colourimetric determining tube of nitrite in water
Alghamdi Determination of allura red in some food samples by adsorptive stripping voltammetry
CN1904617B (en) Free cobalt quantitative determination method of serum blood deficiency decorated albumin
CN1310028C (en) Fluoride assaying liquid and its colorimeteric estimation tube
Szabadvary et al. Development of the pH concept: A historical survey
Peris-Tortajada et al. Determination of reducing sugars by the neocuproine method using flow injection analysis
Star Quantitation of total carbon dioxide in nanoliter samples by flow-through fluorometry
Ala'ddin et al. Individual and simultaneous determination of uric acid and ascorbic acid by flow injection analysis
CN2596351Y (en) Phosphorus detection tube in soil, fertilizer and water
CN2681139Y (en) Fluoride colorimetric determination tube
CN1176368C (en) Colourimetric fast assaying tube for bicarbonate radical in water
JP2824793B2 (en) Simultaneous automatic measurement of cell concentration and cell activity
CN105158181A (en) Kit for detecting fluoride ions
CN2551995Y (en) Fast colorimetric estimation tube for bicarbonate radical in water
CN114295564A (en) Method for determining total phosphorus content in sewage by utilizing microplate reader

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
C17 Cessation of patent right
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20050302

Termination date: 20140208