CN1190489C - Pseudomonads, its preparation method and application - Google Patents
Pseudomonads, its preparation method and application Download PDFInfo
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- CN1190489C CN1190489C CNB031319440A CN03131944A CN1190489C CN 1190489 C CN1190489 C CN 1190489C CN B031319440 A CNB031319440 A CN B031319440A CN 03131944 A CN03131944 A CN 03131944A CN 1190489 C CN1190489 C CN 1190489C
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- 238000002360 preparation method Methods 0.000 title claims abstract description 9
- 241000894006 Bacteria Species 0.000 claims abstract description 31
- 150000002897 organic nitrogen compounds Chemical class 0.000 claims abstract description 20
- 238000011282 treatment Methods 0.000 claims abstract description 13
- 238000004321 preservation Methods 0.000 claims abstract description 4
- 241000589516 Pseudomonas Species 0.000 claims description 33
- 239000002351 wastewater Substances 0.000 claims description 25
- 230000001580 bacterial effect Effects 0.000 claims description 24
- PAYRUJLWNCNPSJ-UHFFFAOYSA-N Aniline Chemical compound NC1=CC=CC=C1 PAYRUJLWNCNPSJ-UHFFFAOYSA-N 0.000 claims description 14
- 239000000243 solution Substances 0.000 claims description 12
- LQNUZADURLCDLV-UHFFFAOYSA-N nitrobenzene Chemical compound [O-][N+](=O)C1=CC=CC=C1 LQNUZADURLCDLV-UHFFFAOYSA-N 0.000 claims description 11
- 235000015097 nutrients Nutrition 0.000 claims description 9
- WTLKTXIHIHFSGU-UHFFFAOYSA-N 2-nitrosoguanidine Chemical compound NC(N)=NN=O WTLKTXIHIHFSGU-UHFFFAOYSA-N 0.000 claims description 8
- 238000009631 Broth culture Methods 0.000 claims description 7
- BFCFYVKQTRLZHA-UHFFFAOYSA-N 1-chloro-2-nitrobenzene Chemical compound [O-][N+](=O)C1=CC=CC=C1Cl BFCFYVKQTRLZHA-UHFFFAOYSA-N 0.000 claims description 6
- 239000001963 growth medium Substances 0.000 claims description 6
- 239000000725 suspension Substances 0.000 claims description 6
- 241000190932 Rhodopseudomonas Species 0.000 claims description 5
- 239000010786 composite waste Substances 0.000 claims description 5
- 238000000034 method Methods 0.000 claims description 5
- 239000010802 sludge Substances 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- 238000012360 testing method Methods 0.000 claims description 4
- IQUPABOKLQSFBK-UHFFFAOYSA-N 2-nitrophenol Chemical compound OC1=CC=CC=C1[N+]([O-])=O IQUPABOKLQSFBK-UHFFFAOYSA-N 0.000 claims description 3
- 241000589774 Pseudomonas sp. Species 0.000 claims description 3
- 238000009395 breeding Methods 0.000 claims description 3
- 230000001488 breeding effect Effects 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- 238000003760 magnetic stirring Methods 0.000 claims description 3
- 244000005700 microbiome Species 0.000 claims description 3
- 230000000877 morphologic effect Effects 0.000 claims description 3
- 231100000219 mutagenic Toxicity 0.000 claims description 3
- 230000003505 mutagenic effect Effects 0.000 claims description 3
- 239000002504 physiological saline solution Substances 0.000 claims description 3
- CDAWCLOXVUBKRW-UHFFFAOYSA-N 2-aminophenol Chemical compound NC1=CC=CC=C1O CDAWCLOXVUBKRW-UHFFFAOYSA-N 0.000 claims 2
- 238000004065 wastewater treatment Methods 0.000 abstract description 14
- 238000004519 manufacturing process Methods 0.000 abstract description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 6
- 125000000449 nitro group Chemical group [O-][N+](*)=O 0.000 abstract description 5
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 abstract description 4
- 230000015556 catabolic process Effects 0.000 abstract description 2
- 238000006731 degradation reaction Methods 0.000 abstract description 2
- 238000000746 purification Methods 0.000 abstract description 2
- 238000002955 isolation Methods 0.000 abstract 2
- 241001148470 aerobic bacillus Species 0.000 abstract 1
- 230000000813 microbial effect Effects 0.000 abstract 1
- 238000009629 microbiological culture Methods 0.000 abstract 1
- 230000008901 benefit Effects 0.000 description 6
- 239000000975 dye Substances 0.000 description 5
- 239000000203 mixture Substances 0.000 description 5
- BLCZXLGRKKRKJJ-UHFFFAOYSA-N 3-amino-2-nitrophenol Chemical compound NC1=CC=CC(O)=C1[N+]([O-])=O BLCZXLGRKKRKJJ-UHFFFAOYSA-N 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 239000001888 Peptone Substances 0.000 description 3
- 108010080698 Peptones Proteins 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 3
- 238000007599 discharging Methods 0.000 description 3
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 3
- 239000010815 organic waste Substances 0.000 description 3
- 235000019319 peptone Nutrition 0.000 description 3
- 241001460678 Napo <wasp> Species 0.000 description 2
- PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 description 2
- 125000003368 amide group Chemical group 0.000 description 2
- 150000001491 aromatic compounds Chemical class 0.000 description 2
- 235000015278 beef Nutrition 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000003203 everyday effect Effects 0.000 description 2
- 210000003495 flagella Anatomy 0.000 description 2
- 239000002054 inoculum Substances 0.000 description 2
- 239000013067 intermediate product Substances 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 230000009257 reactivity Effects 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 235000014347 soups Nutrition 0.000 description 2
- 238000009423 ventilation Methods 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 239000003643 water by type Substances 0.000 description 2
- VGVRPFIJEJYOFN-UHFFFAOYSA-N 2,3,4,6-tetrachlorophenol Chemical class OC1=C(Cl)C=C(Cl)C(Cl)=C1Cl VGVRPFIJEJYOFN-UHFFFAOYSA-N 0.000 description 1
- 239000005631 2,4-Dichlorophenoxyacetic acid Substances 0.000 description 1
- 241000208340 Araliaceae Species 0.000 description 1
- 238000003794 Gram staining Methods 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- WPYMKLBDIGXBTP-UHFFFAOYSA-N benzoic acid Chemical compound OC(=O)C1=CC=CC=C1 WPYMKLBDIGXBTP-UHFFFAOYSA-N 0.000 description 1
- CUBCNYWQJHBXIY-UHFFFAOYSA-N benzoic acid;2-hydroxybenzoic acid Chemical compound OC(=O)C1=CC=CC=C1.OC(=O)C1=CC=CC=C1O CUBCNYWQJHBXIY-UHFFFAOYSA-N 0.000 description 1
- 238000005660 chlorination reaction Methods 0.000 description 1
- -1 chloro aromatic compound Chemical class 0.000 description 1
- 238000013016 damping Methods 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 238000006477 desulfuration reaction Methods 0.000 description 1
- 230000023556 desulfurization Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 231100000350 mutagenesis Toxicity 0.000 description 1
- 238000002703 mutagenesis Methods 0.000 description 1
- VMPITZXILSNTON-UHFFFAOYSA-N o-anisidine Chemical compound COC1=CC=CC=C1N VMPITZXILSNTON-UHFFFAOYSA-N 0.000 description 1
- 150000002894 organic compounds Chemical class 0.000 description 1
- 239000003208 petroleum Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Landscapes
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention belongs to a bacteria class, particularly to Pseudomonads sp and a preparation method thereof and the application in high concentration organic nitrogen compound waste water treatment and particularly to in nitro and amino class organic wastewater treatment. The Pseudomonads sp FD126 is preserved in the General Microbial Centre of Chinese Microbial Culture Preservation Management Committee, and the preservation number is CGMCC No. 0914. The Pseudomonads sp of the present invention are 22 kinds of aerobic bacteria (initial bacteria) with organic nitrogen compound degradation capability with different degrees, and the initial bacteria are sieved from a production device which runs for 20 years. Pseudomonad strains are obtained by isolation, inducement variation, isolation purification and domestication cultivation. In the waste water treatment of a high concentration organic nitrogen compound, particularly the nitro and amino class organic wastewater treatment application, the system runs stably, and the cost is low. The various indexes of the system outlet water after the treatment are superior to GB8978-96 first level standard.
Description
Technical field
The invention belongs to the bacterium class, is in a kind of pseudomonas (Pseudomonas sp.) and preparation method thereof and the high density organic nitrogen compound waste water treatment specifically, special application in nitro, amido class treatment of Organic Wastewater.
Background technology
The existing in the past Rhodopseudomonas (Pseudomonas) of relevant document has the research report in wastewater treatment both at home and abroad, on December 19th, 2000, Hua Zhong Agriculture University and Zhejiang University once applied for a patent, and name is called the pseudomonads GT 241-1-1 and the purposes of degradating chloro aromatic compound and aromatic compound.Inventive features is clear and definite, and this bacterial strain can fully degrade 2,4-two chlorophenols, 2,4-D, phenylformic acid and Whitfield's ointment and salt thereof.But high density organic nitrogen compound waste-water treatment efficient bacterium does not still have record, particularly adopts pseudomonas effectively to handle dye well intermediate class organic waste water and does not see the document record so far.
The application of pseudomonas, just found in biochemical treatment system has Rhodopseudomonas to exist in the past, but is not single cultivation, domestication and application.At present existing indivedual pseudomonas strains are applied in chlorination aromatic compound, sulfocompound desulfurization, the methanol waste water processing.But its range of application is narrow, and specificity is strong, and in high density organic nitrogen compound (dye well intermediate) waste water treatment, in nitro, amido class treatment of Organic Wastewater, uses efficient specificity bacterial strain and use genus blank still in this field of waste water treatment especially.
Production application, the use in waste water treatment bacterial classification of wastewater treatment special bacteria have several companies to sell both at home and abroad both at home and abroad, through checking and verify, all do not have the product of organic nitrogen compound product waste-water treatment efficient special bacterium-Rhodopseudomonas.
Summary of the invention
The present invention is directed to above-mentioned weak point provides a kind of pseudomonas and preparation method thereof, uses, the present invention filters out (initial bacterial classification) 22 kinds of good bacterias with the organic nitrogen compound ability of degrading in various degree from move the production equipment over more than 20 year, after the pseudomonas strain that separation, induce variation, separation and purification, acclimatization culture are come out.
Test experiments through degraded organic nitrogen compound ability, obtain the higher bacterial classification of a strain degradation capability, called after FD126, in 24 hours to aniline, oil of mirbane, nitrophenol, nitro-chlorobenzene, 2, multiple nitrogenous serial organic removal rates such as 4-dinitrochlorobenzene reach 64%～71%, during engineering is used, more than various organic nitrogen compound clearances reach 99.9%, water outlet all is better than the GB8978-1996 primary standard.
Cell characteristic: cell is shaft-like, Gram-negative, having a polar flagellum can move, the temperature of suitable growth is 30～35 ℃, when 3 ℃ and 41 ℃ Metabolic activity a little less than, do not grow for 47～5 ℃, can not grow below the PH4 in the suitableeest growth PH6.5～9.0.
In the broth culture flat board, visible irregular grey shape white colony after 16 hours, the bacterium colony size is 0.2 * 0.5----1cm after 24 hours, this bacterium cell is single, thin rod shape, almost spherical sometimes, gramstaining is negative, and has a flagellum, can move.
This pseudomonas culture presevation at present is at China Committee for Culture Collection of Microorganisms common micro-organisms center, register on the books to number in this preservation center: CGMCC No.0914, the Latin formal name used at school of bacterial classification is: Pseudomonas sp., the microorganism (strain) of ginseng certificate: FD126, preservation date are on March 27th, 2003.
A kind of pseudomonas preparation method:
1, increasing bacterium cultivates
Adopt common bacterium to use broth culture:
Add a certain amount of through nitrobenzene-containing, aniline, nitro-chlorobenzene, amino (nitro) phenol, 2, multiple organic nitrogen compounds such as 4-dinitrochlorobenzene mix and divide waste water, by substratum: the mixed of waste water=1: 4 is made enrichment culture medium, get the active sludge (pseudomonas bacterium liquid) of pseudomonas, be inoculated in the triangular flask of containing nutrient solution, in 20 ℃-32 ℃ following shaking culture 16-26 hour.
2, purifies and separates
Adopt plate streak, single bacterial classification that purifies and separates is come out, increasing bacterium again cultivates, through repetition test, obtain the higher bacterial classification of above-mentioned waste water clearance, measure (" general bacterium commonly used authentication method commonly used ") by bacterial classification morphologic observation and biochemical characteristic and can determine viable bacteria kind Rhodopseudomonas.
3, mutagenic treatment, directive breeding
Bacterium strength of solution 10
7Individual/ml, adopt physiological saline, buffered soln is cultivated.
Adopt ultraviolet ray and nitrosoguanidine successively to carry out mutagenesis for using.Getting above-mentioned bacterium suspension 15ml is placed in the 90ml culture dish, be placed in the darkroom that ultraviolet lamp is housed, on magnetic stirring apparatus, shine 100-140 second while stirring, the back adds nitrosoguanidine (NTG) and handled 20-35 minute, through repeated treatments repeatedly, finally isolate high clearance, the high reactivity specificity bacterial classification of one group of above-mentioned organic components waste water, promptly make pseudomonas.
4, the cultivation of advantage high-efficiency strain
Adopt above-mentioned composite waste to receive broth culture, by waste water: the mixed of nutrient solution=8: 1 becomes enrichment culture medium, adds 5ml obligate bacterium (pseudomonas) suspension and fills in the triangular flask of nutrient solution of 195ml, 28 ℃ of shaking culture 24 hours.
5, bacterial classification production and application
The intermittent type spawn culture reactor of 50L
The intermittent type spawn culture reactor of 100L
Add the active sludge in the original system of polymer alumina and 50% container volume, add waste water COD er1500mg/L and cultivated 36 hours, settlement separate.
Culture condition:
Inoculum size 0.1～5.0% (volume)
Plant 24～36 hours ages
Ventilation 0.3～1.5 volume/volume. divide
From 0 to 650 rev/min of stirring
26～28 ℃ of temperature
PH6.5～8.5
Enrichment medium (beef soup, peptone, NaPO
40.75% (NH
4) SO
4, 0.3%): waste water (organic nitrogen compound)=1: 8
In engineering is used, should adjust said ratio at the different quality composition, more help the raising of bacterial classification to the clearance of special waste.
Outstanding advantage of the present invention:
The present invention compares with prior art in this field, has:
1, this bacterial classification reaches 99.9% to the organic removal rate height, and degraded thoroughly.
2, be easy to cultivate, but life-time service is difficult for degenerating.
3, processing efficiency height, reduced investment.
4, use range is wide, is widely used in the organic nitrogen compound field of wastewater.
Embodiment
A kind of pseudomonas preparation method:
1, bacterium is cultivated
Adopt common bacterium to use broth culture:
Extractum carnis 0.5%, peptone 10%, Nacl0.5%, PH6.5～9.0, add a certain amount of nitrobenzene-containing, aniline, nitro-chlorobenzene, amino (nitro) phenol, 2, multiple organic nitrogen compound composite wastes (CODcr is about 1500mg/L) such as 4-dinitrochlorobenzene, organic content adds up to about 560mg/L, mixed by substratum waste water=1: 4 is made enrichment culture medium, get active sludge (the pseudomonas bacterium liquid) 10ml of pseudomonas, be inoculated in the triangular flask of the nutrient solution of containing 90ml, in 28 ℃ of following shaking culture 24 hours.
2, purifies and separates
Adopt plate streak, single bacterial classification that purifies and separates is come out, increasing bacterium again cultivates, through repetition test, obtain the higher bacterial classification of above-mentioned waste water clearance, measure (" general bacterium commonly used authentication method commonly used ") by bacterial classification morphologic observation and biochemical characteristic and can determine viable bacteria kind Rhodopseudomonas.
3, mutagenic treatment, directive breeding
Bacterium strength of solution 10
7Individual/ml (physiological saline, damping fluid)
Adopting ultraviolet ray successively to replace with nitrosoguanidine uses.Getting above-mentioned bacterium suspension 15ml is placed in the 90ml culture dish, be placed in the darkroom that 15 watts of ultraviolet lamps are housed, apart from fluorescent tube 30cm place, on magnetic stirring apparatus, shone while stirring 120 seconds, the back adds NTG (nitrosoguanidine) 1000 μ g/ml and handled 30 minutes, through repeated treatments repeatedly, finally isolate high clearance, the high reactivity specificity bacterial classification of one group of above-mentioned organic components waste water, promptly make pseudomonas.
4, the cultivation of advantage high-efficiency strain
Adopt above-mentioned nitrobenzene-containing, aniline, nitro-chlorobenzene, amino (nitro) phenol, 2, the multiple organic nitrogen compound composite waste of 4-dinitrochlorobenzene is received broth culture, by waste water: the mixed of nutrient solution=8: 1 becomes enrichment culture medium, add in the triangular flask of 5ml pseudomonas suspension and the nutrient solution that fills 195ml, 28 ℃ of shaking culture 24 hours, the efficient pseudomonas bacterial classification of the advantage of turning out.
5, bacterial classification production and application
The intermittent type spawn culture reactor of 50L
The intermittent type spawn culture reactor of 100L
Add the active sludge in the original system of polymer alumina and 50% container volume, add waste water COD er1500mg/L and cultivated 36 hours, settlement separate.
Culture condition:
Inoculum size 0.1～5.0% (volume)
Plant 24～36 hours ages
Ventilation 0.3～1.5 volume/volume. divide
From 0 to 650 rev/min of stirring
26～28 ℃ of temperature
PH6.5～8.5
Enrichment medium (beef soup, peptone, NaPO
40.75% (NH
4) SO
4, 0.3%): waste water (organic nitrogen compound)=1: 8.
In engineering is used, should adjust said ratio at the different quality composition, more help the raising of bacterial classification to the clearance of special waste.
Application example
At present, this invention pseudomonas has been tried to be applied in nitro class, amine, the amino class wastewater treatment engineering, and treatment unit is stable, and processed waste water is better than the requirement of GB primary standard.Below be pseudomonas kind application example:
One, Hua Yichang dyes in the city in Henan Province
This factory owner produces 2, and the 4-dinitrochlorobenzene belongs to the organic dye intermediate product, in process of production, 300 tons of nitrogenous organic waste waters of concentration of washing process discharging every day, 250 tons of waste water of the same race of spent acid concentration technology discharging, after the mixing, intractability is quite big.This factory adopts the present invention, builds up a cover biochemical treatment apparatus and drops into trial run, and system is stable, and cost is lower, handles the water outlet of back system and is better than the GB8978-96 primary standard.
Two, Henan Province's petroleum chemical plant
This factory owner produces, Ortho Anisidine, belongs to organic amino dyestuff intermediate product, in process of production, 100 tons of nitrogenous organic waste waters of high density of washing process discharging every day, this waste water salt concentration height, organic composition is many and mix, and intractability is quite big.This factory adopts the present invention, builds up a cover and adopts pseudomonas biochemical treatment apparatus of the present invention and drop into trial run, and system is stable, and cost is lower, handles the every index of back system's water outlet and is better than the GB8978-96 primary standard.
Three, Jiangsu Province city company
The said firm (corporation with foreign capital) adopts the present invention to build up a cover and adopts pseudomonas of the present invention, day is handled organic compound wastewater treatment equipments such as 200 tons of nitrobenzene-containings, aniline, through trial run, system is stable, cost is lower, handles the every index of back system's water outlet and is better than the GB8978-96 primary standard.
The present invention obtains reasonable effect in practical engineering application, pseudomonas of the present invention is used in handling high density organic nitrogen compound waste water, has a extensive future, and social benefit and environmental benefit are very remarkable.
Claims (4)
1, a kind of pseudomonas Pseudomonas sp.FD126, it is in China Committee for Culture Collection of Microorganisms's common micro-organisms center preservation, and its deposit number is: CGMCC No.0914.
2, the preparation method of the described a kind of pseudomonas of claim 1 is characterized in that:
(1) increasing bacterium cultivates
Adopt common bacterium to use broth culture, add nitrobenzene-containing, aniline, nitro-chlorobenzene, amino phenol, nitrophenol, 2, the multiple organic nitrogen compound composite waste of 4-dinitrochlorobenzene, by substratum: the mixed of waste water=1: 4 is made enrichment culture medium, the active sludge of getting pseudomonas is a pseudomonas bacterium liquid, be inoculated in the triangular flask of nutrient solution, in 20 ℃-32 ℃ following shaking culture 16-26 hour;
(2) purifies and separates
Adopt plate streak, with single bacterial classification that purifies and separates is come out, increase bacterium again and cultivate, through repetition test, obtain the bacterial classification of handling above-mentioned waste water, measure by bacterial classification morphologic observation and biochemical characteristic and adopt general bacterium commonly used authentication method commonly used can determine viable bacteria kind Rhodopseudomonas;
(3) mutagenic treatment, directive breeding
Bacterium strength of solution 10
7Individual/ml, adopt physiological saline, buffered soln is cultivated, adopt ultraviolet ray successively to replace and use, get above-mentioned bacterium suspension and be placed in the culture dish, be placed in the darkroom that ultraviolet lamp is housed with nitrosoguanidine, on magnetic stirring apparatus, shine 100-140 second while stirring, the back adds nitrosoguanidine handled 20-35 minute, through repeated treatments repeatedly, finally isolated above-mentioned pseudomonas.
3, the preparation method of a kind of pseudomonas according to claim 2, it is characterized in that adopting nitrobenzene-containing, aniline, nitro-chlorobenzene, amino phenol, nitrophenol, 2, the multiple organic nitrogen compound composite waste of 4-dinitrochlorobenzene is received broth culture, by waste water: the mixed of nutrient solution=8: 1 becomes enrichment culture medium, add in the triangular flask of 5ml pseudomonas suspension and the nutrient solution that fills 195ml, 28 ℃ of shaking culture 24 hours, turn out above-mentioned pseudomonas bacterial classification.
4, the described a kind of pseudomonas of claim 1 is used in handling high density organic nitrogen compound waste water.
Priority Applications (1)
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| CNB031319440A CN1190489C (en) | 2003-06-20 | 2003-06-20 | Pseudomonads, its preparation method and application |
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|---|---|---|---|
| CNB031319440A CN1190489C (en) | 2003-06-20 | 2003-06-20 | Pseudomonads, its preparation method and application |
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| CN1190489C true CN1190489C (en) | 2005-02-23 |
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Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100655030B1 (en) * | 2005-11-24 | 2006-12-06 | 주식회사 효성 | -72 26- Pseudomonas sp. HN-72 and Purification method of 26-naphthalene dicarboxylic acid using the same |
| CN100427585C (en) * | 2006-08-24 | 2008-10-22 | 浙江大学 | Bacterial strain of reduction complexing motric oxide and its use |
| EP2218773A1 (en) * | 2009-02-17 | 2010-08-18 | Deinove | Compositions and methods for degrading lignocellulosic biomass |
| CN101781024B (en) * | 2009-12-01 | 2012-02-29 | 吉林大学 | Composite microbial inoculum for quickly degrading nitrobenzene and phenylamine, and preparation method and application thereof |
| CN101935622B (en) * | 2010-02-05 | 2012-01-11 | 北京大学 | Pseudomonas strain and application thereof |
| CN111269863B (en) * | 2020-03-17 | 2022-06-10 | 山东中农普宁药业有限公司 | Pseudomonas and application thereof |
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