CN118786224A - 用于核酸扩增的内部对照 - Google Patents
用于核酸扩增的内部对照 Download PDFInfo
- Publication number
- CN118786224A CN118786224A CN202380021560.2A CN202380021560A CN118786224A CN 118786224 A CN118786224 A CN 118786224A CN 202380021560 A CN202380021560 A CN 202380021560A CN 118786224 A CN118786224 A CN 118786224A
- Authority
- CN
- China
- Prior art keywords
- quality control
- control primer
- amplification
- primer
- nucleic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6846—Common amplification features
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/166—Oligonucleotides used as internal standards, controls or normalisation probes
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202263309827P | 2022-02-14 | 2022-02-14 | |
| US63/309,827 | 2022-02-14 | ||
| PCT/US2023/062485 WO2023154920A1 (en) | 2022-02-14 | 2023-02-13 | Internal controls for nucleic acid amplification |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN118786224A true CN118786224A (zh) | 2024-10-15 |
Family
ID=85640767
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202380021560.2A Pending CN118786224A (zh) | 2022-02-14 | 2023-02-13 | 用于核酸扩增的内部对照 |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US20250051837A1 (https=) |
| EP (1) | EP4479556B1 (https=) |
| JP (1) | JP2025507309A (https=) |
| CN (1) | CN118786224A (https=) |
| WO (1) | WO2023154920A1 (https=) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2025161028A1 (zh) * | 2024-02-04 | 2025-08-07 | 深圳华大智造科技股份有限公司 | 引物及其在lamp扩增产物检测中的应用 |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9617587B1 (en) | 2016-04-04 | 2017-04-11 | Nat Diagnostics, Inc. | Isothermal amplification components and processes |
| WO2019163064A1 (ja) * | 2018-02-22 | 2019-08-29 | 学校法人 慶應義塾 | Pcr成否判定法 |
-
2023
- 2023-02-13 WO PCT/US2023/062485 patent/WO2023154920A1/en not_active Ceased
- 2023-02-13 CN CN202380021560.2A patent/CN118786224A/zh active Pending
- 2023-02-13 JP JP2024546274A patent/JP2025507309A/ja active Pending
- 2023-02-13 EP EP23711345.1A patent/EP4479556B1/en active Active
- 2023-02-13 US US18/721,632 patent/US20250051837A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| EP4479556A1 (en) | 2024-12-25 |
| EP4479556B1 (en) | 2026-03-04 |
| WO2023154920A1 (en) | 2023-08-17 |
| JP2025507309A (ja) | 2025-03-18 |
| US20250051837A1 (en) | 2025-02-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US20220033891A1 (en) | Amplification with primers of limited nucleotide composition | |
| CN105339505B (zh) | 基于通用报告体的基因分型方法和材料 | |
| JP5680078B2 (ja) | ライゲーションに基づく核酸の正規化した定量化方法 | |
| EP3207156A1 (en) | Methods, kits & compositions for determining gene copy numbers | |
| KR20120107501A (ko) | 오류 시그널이 배제되는 타겟 핵산서열의 실시간 멀티플렉싱 검출 | |
| CN113832258A (zh) | 使用衰减型探针的核糖核酸扩增和检测 | |
| US20190203269A1 (en) | Tri-nucleotide rolling circle amplification | |
| EP4479556B1 (en) | Internal controls for nucleic acid amplification | |
| US20260055445A1 (en) | Archaeal polymerase amplification | |
| JP2025541744A (ja) | 核酸検出のための非対称性ヘアピンプローブ | |
| WO2014161975A1 (en) | Method for performing a melting curve analysis | |
| US20260055448A1 (en) | Hairpin internal control for isothermal nucleic acid amplification | |
| CN119948173A (zh) | 用于改进检测特异性的修饰的分子信标 | |
| WO2018009677A1 (en) | Fast target enrichment by multiplexed relay pcr with modified bubble primers | |
| HK40064470A (en) | Amplification with primers of limited nucleotide composition | |
| KR20240058025A (ko) | 대장균의 o110, o113, o167 또는 o116 혈청형 신속 동정용 프라이머 세트 및 이를 이용한 대장균의 혈청형 동정 방법 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |