CN1186959A - Immune, chemical and biochemical method for identifying anti-fake product - Google Patents
Immune, chemical and biochemical method for identifying anti-fake product Download PDFInfo
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- CN1186959A CN1186959A CN 97121957 CN97121957A CN1186959A CN 1186959 A CN1186959 A CN 1186959A CN 97121957 CN97121957 CN 97121957 CN 97121957 A CN97121957 A CN 97121957A CN 1186959 A CN1186959 A CN 1186959A
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Abstract
The present invention relates to a method using one step method test strip to simply and accurately appraise the false or true of certain kind of product, these products consist of wine, tobacco, medicine, nutriment and high profit goods, banknotes, cheque, receipt, confidential article and important documents. The one step checking test strip made by the immune, chemical and biochemical method utilizes the characteristic of identifiable peculiar property between the biomolecules and high affinity force combination. One or multiple kinds of peculiar property component, molecule or matter contained in certain product can be used to identificate the true or false of the article to be appraised . Said invention finds out or adds in one or more peculiar component in certain product and makes it and the other matter (antibody, receiptor etc.) to do peculiar property identification or combination, then the one step method checking test strip is used for identification.
Description
The invention belongs to biological technical field.
Differentiate the true and false of product, normally difficult, especially a kind of valuable product or raw material, often by the personation, from health products, medicine, tobacco to banknote, normally discern the false from the genuine very much, fake products has brought great harm to society, and therefore false proof and false distinguishing work just seems extremely important.
At present, the true and false of generally differentiating product all is to discern by the method for physics, and will carry out with special instrument and well-trained people.False distinguishing work difficulty in other words is bigger, so just gives personation the convenience of having opened of poor quality.
The purpose of this invention is to provide a kind of utilization immunity, chemistry, biochemical new method evaluation anti-fake product, this method is simple to operate, weak point consuming time and reliable results.
The present invention finishes by following technical proposals:
Based on the high specific and the high sensitivity of biological respinse, the specific recognition of utilizing biomolecule with combine, as antigen and antibody, acceptor and part, enzyme-to-substrate, complementary nucleic acid etc. surpass hundreds thousand of kinds, as are put to artificial modification and then can make selection above 1,000,000 kinds with accurate operation.Select certain identification and association reaction in advance, or several idiosyncrasies are combined,, we can say it is to decode wherein difficult to understand close basically as long as the deviser initiatively do not reveal.
The present invention at first finds out in original product or raw material and adds one or more distinctive compositions, and this may be a kind of molecule, biological substance, compound or physical aspect.These materials all have specific steric configuration and can be other material (as antibody, acceptor etc.) institute's specific recognition and combination.Detect strip with single stage method of the present invention then and identify above predetermined substance.
The single stage method strip is according to principles such as immunology, molecular biology, adopts biochemical method to make, in the prior art in clinical use (as detecting hepatitis B and whether conceivedly predicting).
Be used as among the present invention and identify that anti-fake product can adopt following two kinds of methods to carry out:
1, use immunization method, these peculiar materials can make monoclonal, polyclonal antibody, are prepared into the single stage method strip again, and Color Appearance System such as collaurum or coloured latex Color Appearance System are used as single stage method detection strip.
2. with chemical, biochemical method, determine the chemistry or the biochemical reaction of relevant peculiar material, make the single stage method strip together with Color Appearance System then.
Single stage method strip detection technique is not only very convenient but also very sensitive, can detect nanogram (ng) level, and very economical, helps promoting the use of.
Now engage description of drawings embodiment:
One, single stage method detection strip discriminating counterfeit money and check, vital document, proof etc.
The method of banknote of being used for now discerning the false from the genuine is to differentiate by physics, detects as uviol lamp or to the structure of bank note, and this method all requires certain instrument and professional.The invention conception that detects strip with single stage method just can be used for reaching above discriminating purpose.It is that one or more antigenic substances are added in the paper of making banknote, as bovin serum albumin (BSA), so every bank note all contains this distinctive antigen, detect strip (containing corresponding antibody) with single stage method of the present invention and detect whether contain this peculiar material, just can pick out the true and false of bank note.
The principle (using immunization method) that single stage method detects:
The single stage method immunization detect strip be one and fluid samples in checking matter responsive detection is provided.Single stage method immune detection strip constitutes (see figure 1) by following three parts, and the A. solid support is combined with part or acceptor on support, be generally antibody, antigen or haptens.Support is porous material such as nitre resin acid tunica fibrosa etc. normally.As measured object is BSA, just is coated with the monoclonal antibody of BSA on support.B. test section, normally porous material such as nylon, glass fibre are coated with the monoclonal antibody of the anti-BSA of collaurum target.The BSA site that this antibody resisted and the antibody of solid support are different.C. buffer zone.
(see figure 2) during use, strip B section is inserted in the determinand solution, (banknote to be checked can be dipped into the several seconds in the solution box with one jiao, can obtain determinand solution) during this, if sample has measured object (BSA), just can with the collaurum target antibodies of B section, in conjunction with after then can seepage flow to the A section, complete with the monoclonal antibody body knot that is coated on the A section, and generate " sandwich " complex.There is not the material of combination then to be passed to top C section along the A section.Because collaurum is the colour developing product, the compound that rests on the A section antibody can produce a red zone, and the existence of checking matter is described.
On solid is supported, an other contrast index band is arranged, be coated with anti-immunoglobulin G, no matter there is checking matter in sample, collaurum target antibody also can be followed its combination, produces control line, and is working properly with the proof strip.
In making the bank note process, can use more than a kind of micro-antigen combination and add in the bank note paper, simultaneously also can add inhibitory substance, on the detection strip, cause the associativity positive or inhibition positive reaction, because the extreme complicacy of biomolecule steric configuration, required antigenic substance is trace extremely, or only add and be printed on the privileged site for the treatment of false proof object, the objects of different numberings also can be used different antigens combinations instead.When the preparation test-strips, corresponding monoclonal antibody be need develop simultaneously and combination and relevant complicated technology detected, all need a suitable process, therefore, as long as do not announce micro-antigen prescription and default accordingly indicia patterns in the corresponding Serial Number Range, it almost is impossible attempting to copy this system.So can not find out the equation that immunity causes material, also just be difficult to copy same bank note certainly.
Two, single stage method detects the true and false that strip is identified nutriment, medicine
Comprised many valuable compositions in the nutriment,, be easier to, therefore be difficult to distinguish its whether genuine goods at a fair price by cheap composition personation as genseng etc.
Detect the conception that strip is invented with single stage method, can discern the true and false of nutriment.The checking matter that at first adds the last layer trace on the surface of nutriment or medicine (on the capsule), as BSA (bovin serum albumin), the single stage method strip made from the monoclonal antibody of anti-bovin serum albumin detects then, and this strip can be distinguished the true and false of nutriment very accurately.
Another method is to propose one or more peculiar materials, the monoclonal antibody of making the peculiar material of antibody then of immunization in medicine or nutriment.Make the single stage method strip with this antibody again.
When extracting peculiar material, can rely on high pressure liquid phase spectrum (HPLC), the material of different molecular weight and different characteristic in nutriment or the medicine separately, when making the strip of monoclonal antibody, can select a different set of material for use, make imitation more difficult.
Three, single stage method detects the true and false that strip is identified famous brand of wine
Before famous brand of wine dispatches from the factory, can add one or more measured objects at the ad-hoc location of bottle or bottle cap, use principle of uniformity, the single stage method of anti-bovin serum albumin detects the true and false that strip can be discerned wine, the manufacturer also can add measured object in wine artificially, and does not influence the quality of wine.Or find out the specific monoclonal antibody of anti-this wine with immunization, and cooperated the single stage method strip to detect, just can identify.
Fortune uses the same method and can add detected material at computer optical disc, certificate, passport, famous mark, seal, stamp-pad ink etc., differentiates measured object with the single stage method strip and whether exists, and just knows the true and false.
Advantage of the present invention:
1, identify that true and false product is highly sensitive, reliability is higher.
2, easy and simple to handle, save time, to go out the result fast.
3, need not specific apparatus.
Description of drawings:
Fig. 1 detects the strip synoptic diagram for single stage method.
Fig. 2 is the banknote evaluation operation chart.
Claims (3)
1, be used to identify the immunity of anti-fake product, chemistry, biochemical new method, use single stage method to detect strip, it is characterized in that this method at first finds out in product to be checked or raw material, or add one or more peculiar compositions, such as a kind of molecule, biological substance, these materials of compound or physical aspect have specific space configuration and can for other material (as antibody, acceptor etc.) institute's specific recognition and combination, with immunization method these peculiar materials are made monoclonal then, polyclonal antibody, and make and adopt behind the single stage method strip Color Appearance System to show testing result, perhaps determine to make the single stage method strip together with Color Appearance System behind the chemistry of peculiar material or the biochemical reaction with the biochemical method of chemistry.
2, immunity, chemistry, the biochemical new method that is used to identify anti-fake product as claimed in claim 1 is characterized in that described single stage method immune detection strip is made up of solid Zhi Teti, test section and buffer zone.
3, immunity, chemistry, the biochemical new method that is used to identify anti-fake product as claimed in claim 1 is characterized in that Color Appearance System can be collaurum or coloured latex.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 97121957 CN1186959A (en) | 1997-11-28 | 1997-11-28 | Immune, chemical and biochemical method for identifying anti-fake product |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 97121957 CN1186959A (en) | 1997-11-28 | 1997-11-28 | Immune, chemical and biochemical method for identifying anti-fake product |
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| Publication Number | Publication Date |
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| CN1186959A true CN1186959A (en) | 1998-07-08 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 97121957 Pending CN1186959A (en) | 1997-11-28 | 1997-11-28 | Immune, chemical and biochemical method for identifying anti-fake product |
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| CN (1) | CN1186959A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8703068B1 (en) | 2009-03-04 | 2014-04-22 | The Procter & Gamble Company | Counterfeit detection kit |
-
1997
- 1997-11-28 CN CN 97121957 patent/CN1186959A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8703068B1 (en) | 2009-03-04 | 2014-04-22 | The Procter & Gamble Company | Counterfeit detection kit |
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