CN118239607A - Method for improving nitrite nitrogen conversion effect in half-way denitrification process - Google Patents
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Abstract
The invention discloses a method for improving nitrite nitrogen conversion effect in a half-way denitrification process, which comprises the following steps: inoculating denitrifying bacteria for activation culture, inoculating the activated microbial inoculum into a reactor, adding sulfur-nitrogen-containing wastewater, adjusting the molar ratio of sulfur to nitrogen in the reactor to be 0.675-6:1 and the mass ratio of carbon to nitrogen to be 1.02-2.04:1, and performing half-way denitrification reaction under the anoxic condition. According to the invention, by adding sulfur-nitrogen-containing wastewater, adjusting the sulfur-nitrogen mole ratio and the carbon-nitrogen mass ratio in the system and performing the half-way denitrification reaction under the anoxic condition, the denitrification effect of denitrifying bacteria can be effectively regulated and controlled, the nitrite nitrogen conversion effect in the half-way denitrification process can be improved, the generation rate and conversion rate of nitrite nitrogen in the system can be effectively controlled, and stable and efficient electron acceptors can be provided for the half-way denitrification-anaerobic ammonia oxidation process according to actual needs, so that the efficient and deep denitrification of sewage can be realized.
Description
Technical Field
The invention belongs to the technical field of biological sewage treatment, and particularly relates to a method for improving nitrite nitrogen conversion effect in a half-way denitrification process.
Background
In recent years, the discharge of high nitrogen and sulfur wastewater has severely contaminated groundwater, river water, lake water, and other surface waters, with groundwater being particularly serious. The nitrate nitrogen has high solubility in water and good stability, is difficult to form coprecipitation or adsorb and remove, so that the nitrate nitrogen is easy to diffuse, the nitrate content of tap water in many places is seriously exceeded, and the nitrate nitrogen not only can harm human health, but also can destroy ecological environment. After entering a human body, nitrate nitrogen (NO 3 - -N) can be converted into nitrite nitrogen (NO 2 - -N), wherein nitrite can be combined with protein, so that functions of oxygen transmission and the like are affected, anoxic asphyxia syncope is caused, a series of strong cancerogenic substances which are extremely unfavorable to human health can be formed after long-term intake, and cancers are caused. The excessive nitrogen in natural water body can cause eutrophication, the water body is blackened and smelly, so that algae are propagated in large quantity, water bloom red tide and the like appear, and the environment protection is threatened. The high-nitrogen and high-sulfur waste water such as landfill leachate, leather waste water and the like has the problems of high nitrate nitrogen concentration, low carbon nitrogen ratio, high toxicity, difficult treatment and the like. Therefore, the prevention and control of nitrate nitrogen in high-nitrogen and high-sulfur wastewater are extremely important, and finding out a set of schemes for efficiently reducing the content of nitrate nitrogen in wastewater is a hot spot problem in sewage treatment.
The existing high-nitrogen high-sulfur wastewater treatment process is usually to directly reduce nitrate nitrogen into N 2 and convert sulfate into elemental sulfur, but aeration and additional sulfide addition are needed in the whole process, so that the process is complex, the cost is high, and the wide use of the process is limited. The anaerobic ammonia oxidation process can carry out high-efficiency denitrification on the premise of effectively saving the cost of aeration and carbon sources and greatly shortening the intermediate steps, and how to provide stable nitrite for anaerobic ammonia oxidation bacteria becomes an urgent problem to be solved. However, in the existing anaerobic ammonia oxidation process, the generation rate and conversion rate of nitrite nitrogen are difficult to effectively regulate and control, so that the overall denitrification effect of the anaerobic ammonia oxidation process is still poor. Therefore, how to obtain a method for improving the nitrite nitrogen conversion effect in the half-way denitrification process has important significance for realizing the deep denitrification of the nitrate nitrogen wastewater.
Disclosure of Invention
The invention aims to solve the technical problem of overcoming the defects of the prior art and providing a method for improving the nitrite nitrogen conversion effect in the half-way denitrification process.
In order to solve the technical problems, the invention adopts the following technical scheme.
A method for improving nitrite nitrogen conversion effect in a half-way denitrification process comprises the following steps:
s1, inoculating denitrifying bacteria into an activation culture medium, and performing activation culture to obtain an activated microbial inoculum;
S2, inoculating the activated microbial inoculum into a reactor, adding sulfur-nitrogen-containing wastewater, adjusting the molar ratio of sulfur to nitrogen in the reactor to be 0.675-6:1 and the mass ratio of carbon to nitrogen to be 1.02-2.04:1, and performing half-way denitrification reaction under the anoxic condition.
The method, further improved, further comprises the steps of:
s3, according to the sulfur-nitrogen molar ratio in the reactor, combining a linear regression equation of the sulfur-nitrogen molar ratio in the reactor and the highest removal average speed of nitrate nitrogen, and obtaining the highest removal average speed of the nitrate nitrogen in the reaction system; the linear regression equation of the sulfur-nitrogen molar ratio and the nitrate nitrogen removal rate in the reactor is shown as a formula (1);
y1＝0.695×x1+5.607 (1)；
In the formula (1), y1 is the highest removal average speed of nitrate nitrogen, the unit is mg.L -1·h-1, x1 is the sulfur-nitrogen molar ratio, and the correlation coefficient is R 2 = 0.9765.
The method, further improved, further comprises the steps of:
S4, according to the molar ratio of sulfur to nitrogen in the reactor, and in combination with a linear regression equation of the molar ratio of sulfur to nitrogen in the reactor and the nitrate nitrogen removal rate, the nitrate nitrogen removal rate in the reaction system is obtained; the linear regression equation of the sulfur-nitrogen molar ratio and the nitrate nitrogen removal rate in the reactor is shown as a formula (2);
y2＝-0.704×x2+80.602 (2)；
In the formula (2), y2 is the nitrate nitrogen removal rate, the unit is that x2 is the sulfur nitrogen molar ratio, and the correlation coefficient R 2 =0.9973.
The method, further improved, further comprises the steps of:
S5, according to the removal rate and the removal rate of the nitrate nitrogen, combining a linear regression equation of the removal rate and the removal rate of the nitrate nitrogen with the molar ratio of sulfur and nitrogen in the reactor, and obtaining the molar ratio of sulfur and nitrogen in the reaction system; the linear regression equation of the nitrate nitrogen removal rate, the removal rate and the sulfur-nitrogen molar ratio in the reactor is shown in a formula (3);
X＝-1.922×Y1^0.5-1.765×Y2+146.8753 (3)；
Wherein X is a sulfur-nitrogen molar ratio, Y 1 is a maximum removal average rate of nitrate nitrogen, the unit is mg·l -1·h-1,Y2 is a nitrate nitrogen removal rate, the unit is a correlation coefficient R 2 =0.9987.
In the method, when the molar ratio of sulfur to nitrogen in the reactor is 0.675-2.5:1, the time of the half-way denitrification reaction is more than or equal to 45 hours; or when the molar ratio of sulfur to nitrogen in the reactor is 2.5-6:1, the time of the half-way denitrification reaction is 5-45 h.
In the method, in step S2, the sulfur-containing and nitrogen-containing wastewater is at least one of landfill leachate, leather wastewater and pharmaceutical wastewater; the sulfur-nitrogen-containing wastewater contains nitrate, thiosulfate, carbonate and potassium salt, wherein the concentration of the nitrate in the sulfur-nitrogen-containing wastewater is 0.911g/L, the concentration of the thiosulfate is 1.329 g/L-10.637 g/L, the concentration of the carbonate is 1g/L, the concentration of the potassium salt is 0.636g/L, the nitrate is NaNO 3, the thiosulfate is Na 2S2O3·5H2 O, the carbonate is NaHCO 3, and the potassium salt is KCl.
In the method, in a further improved step S2, the volume ratio of the activated microbial inoculum to the sulfur-nitrogen-containing wastewater is 0.1-0.15:1, and the anoxic condition is that nitrogen is continuously introduced.
In a further improvement of the above method, in step S1, the activation medium comprises the following components: 10.0g/L peptone, 5.0g/L yeast powder, 5.0g/L sodium chloride; the pH value of the activation culture medium is 6.8-7.2.
In the above method, further improved, in step S1, the temperature of the activation culture is 25 ℃ to 29 ℃, and the time of the activation culture is 3 days to 5 days.
Compared with the prior art, the invention has the advantages that:
Aiming at the defects that the nitrite nitrogen conversion effect is difficult to effectively regulate and control in the existing anaerobic ammonia oxidation process, the invention creatively provides a method for improving the nitrite nitrogen conversion effect in the half-process denitrification process, in a denitrifying bacteria culture system, sulfur-nitrogen-containing wastewater is added, the molar ratio of sulfur to nitrogen in the system is regulated to be 0.675-6:1, the mass ratio of carbon to nitrogen is 1.02-2.04:1, and the half-process denitrification reaction is carried out under the anoxic condition, so that the denitrification effect of the denitrifying bacteria can be effectively regulated and controlled, the nitrite nitrogen conversion effect in the half-process denitrification process can be improved, the generation rate and conversion rate of nitrite nitrogen in the system can be effectively controlled, and stable and efficient electron acceptors can be provided for the half-process denitrification-anaerobic ammonia oxidation process according to actual needs, and finally, the efficient and deep denitrification of sewage can be realized.
Drawings
FIG. 1 is a graph showing the concentration change of nitrate nitrogen during the half-denitrification reaction in examples 1-4 of the present invention.
FIG. 2 is a graph showing the change in the average removal rate of nitrate nitrogen during the half-denitrification reaction at different sulfur to nitrogen molar ratios in examples 1-4 of the present invention.
FIG. 3 is a graph showing the change in nitrate nitrogen removal rate during the half-denitrification reaction in examples 1 to 4 according to the present invention.
FIG. 4 is a graph showing the instantaneous removal rate of nitrate nitrogen during the half-denitrification reaction at different sulfur to nitrogen molar ratios in examples 1-4 of the present invention.
FIG. 5 is a graph showing the change in nitrite nitrogen concentration during the course of the half-denitrification reaction at different sulfur to nitrogen molar ratios in examples 1-4 of the present invention.
FIG. 6 is a graph showing the average rate of formation of nitrite nitrogen during the course of the half-denitrification reaction at different sulfur to nitrogen molar ratios in examples 1-4 of the present invention.
FIG. 7 is a graph showing the change in the formation rate of nitrite nitrogen during the course of half-denitrification in examples 1-4 according to the present invention.
FIG. 8 is a graph showing the instantaneous rate of nitrite nitrogen formation during the course of the half-denitrification reaction at different sulfur to nitrogen molar ratios in examples 1-4 of the present invention.
FIG. 9 is a graph of quantitative sulfur to nitrogen ratio versus nitrate nitrogen conversion during a half-way denitrification reaction according to the present invention.
FIG. 10 is a graph of quantitative model of sulfur to nitrogen ratio versus nitrate nitrogen conversion during the course of the half-denitrification reaction of the present invention.
Detailed Description
The invention is further described below in connection with the drawings and the specific preferred embodiments, but the scope of protection of the invention is not limited thereby. The materials and instruments used in the examples below are all commercially available.
Example 1:
the method for improving the nitrite nitrogen conversion effect in the half-way denitrification process comprises the following steps of:
(1) Inoculating denitrifying bacteria into an activation culture medium, and independently culturing for 3-5 days at the temperature of 27 ℃ to obtain an activated microbial inoculum. Wherein the activation medium comprises the following components: 10.0g/L peptone, 5.0g/L yeast powder and 5.0g/L sodium chloride; pH of the activation medium=7.0±0.2.
The denitrifying bacteria adopted in the example are thiobacillus thiooxidans (Ciceribacter thiooxidans) strains in China patent document CN 115820466A.
(2) Adding KCl and NaNO 3、Na2S2O3·5H2O、NaHCO3 into water to obtain a mixed solution, wherein the concentration of KCl in the mixed solution is 0.636g/L, naNO 3, the concentration of KCl in the mixed solution is 0.911g/L, na 2S2O3·5H2 O, the concentration of KCl in the mixed solution is 1.435g/L, naHCO 3, and the mixed solution is used as simulated sulfur and nitrogen-containing wastewater.
(3) Inoculating the activated microbial inoculum into a reactor according to the volume ratio of the activated microbial inoculum to the simulated sulfur-nitrogen-containing wastewater of 0.1:1, adding the simulated sulfur-nitrogen-containing wastewater, adjusting the sulfur-nitrogen molar ratio (namely M (S 2O3 2-)∶M(NO3 -)) in the reactor to be 0.675:1, the carbon-nitrogen mass ratio (namely the mass ratio of NaHCO 3 -C to NaNO 3 -N) to be 1.02:1, introducing N 2, performing half-way denitrification reaction for 100h at 27 ℃, and controlling the pH value of the system to be 7.0-8.0 in the half-way denitrification reaction process.
Example 2:
The method for improving the nitrite nitrogen conversion effect in the half-denitrification process of the invention is basically the same as the method of example 1, except that: in the step (2), the concentration of Na 2S2O3·5H2 O is 2.658g/L, and the molar ratio of sulfur to nitrogen in the reactor is regulated to be 1.25:1.
Example 3:
the method for improving the nitrite nitrogen conversion effect in the half-denitrification process of the invention is basically the same as the method of example 1, except that: in the step (2), the concentration of Na 2S2O3·5H2 O is 5.316g/L, and the molar ratio of sulfur to nitrogen in the reactor is adjusted to be 2.5:1.
Example 4:
The method for improving the nitrite nitrogen conversion effect in the half-denitrification process of the invention is basically the same as the method of example 1, except that: in the step (2), the concentration of Na 2S2O3·5H2 O is 10.632g/L, and the molar ratio of sulfur to nitrogen in the reactor is adjusted to be 5:1.
FIG. 1 is a graph showing the concentration change of nitrate nitrogen during the half-denitrification reaction in examples 1-4 of the present invention. FIG. 2 is a graph showing the change in the average removal rate of nitrate nitrogen during the half-denitrification reaction at different sulfur to nitrogen molar ratios in examples 1-4 of the present invention. FIG. 3 is a graph showing the change in nitrate nitrogen removal rate during the half-denitrification reaction in examples 1 to 4 according to the present invention. FIG. 4 is a graph showing the instantaneous removal rate of nitrate nitrogen during the half-denitrification reaction at different sulfur to nitrogen molar ratios in examples 1-4 of the present invention. FIG. 5 is a graph showing the change in nitrite nitrogen concentration during the course of the half-denitrification reaction at different sulfur to nitrogen molar ratios in examples 1-4 of the present invention. FIG. 6 is a graph showing the average rate of formation of nitrite nitrogen during the course of the half-denitrification reaction at different sulfur to nitrogen molar ratios in examples 1-4 of the present invention. FIG. 7 is a graph showing the change in the formation rate of nitrite nitrogen during the course of half-denitrification in examples 1-4 according to the present invention. FIG. 8 is a graph showing the instantaneous rate of nitrite nitrogen formation during the course of the half-denitrification reaction at different sulfur to nitrogen molar ratios in examples 1-4 of the present invention. Referring to FIGS. 1 to 8, when the molar ratio of sulfur to nitrogen in the reactor is 0.675:1, 1.25:1, 2.5:1, 5:1, respectively, after the half-stage denitrification reaction is performed for 100 hours, the nitrate nitrogen concentration in the wastewater is 27.900 mg.L -1、29.175mg·L-1、29.575mg·L-1、34.350mg·L-1, the corresponding removal rates are 80.21%,79.64%,78.82%,77.1%, the corresponding maximum average removal average rates of the nitrate nitrogen are 5.845mg·L-1·h-1、6.596mg·L-1·h-1、7.565mg·L-1·h-1、8.973mg·L-1·h-1., and simultaneously, the nitrite nitrogen concentration gradually increases along with the progress of the half-stage denitrification reaction; when the molar ratio of sulfur to nitrogen in the reactor is 0.675:1, 1.25:1, 2.5:1 and 5:1, the corresponding maximum concentration of nitrite nitrogen is 101.50 mg.L -1、96.25mg·L-1、90.75mg·L-1、98.75mg·L-1 respectively. Therefore, by adding the sulfur-nitrogen-containing wastewater and adjusting the molar ratio of sulfur to nitrogen in the reactor to be 0.675:1, 1.25:1, 2.5:1 and 5:1, the method is favorable for converting nitrate nitrogen into more nitrite nitrogen, particularly, when the molar ratio of sulfur to nitrogen in the reactor is 0.675-2.5:1, after half denitrification reaction for 45 hours, more excellent nitrite nitrogen conversion effect can be obtained, and when the molar ratio of sulfur to nitrogen in the reactor is 2.5-6:1, more excellent nitrite nitrogen conversion effect can be obtained after half denitrification reaction for 5-45 hours, which shows that the method can improve nitrite nitrogen conversion effect in the half denitrification process.
In addition, according to the data of the removal rate and the removal rate of the nitrate nitrogen in the half denitrification reaction process under different sulfur-nitrogen molar ratios in fig. 1-4, a linear regression equation of the sulfur-nitrogen molar ratio and the removal rate of the nitrate nitrogen in the reactor and a linear regression equation of the sulfur-nitrogen molar ratio and the removal rate of the nitrate nitrogen in the reactor are respectively constructed, as shown in fig. 9.
FIG. 9 is a graph of quantitative sulfur to nitrogen ratio versus nitrate nitrogen conversion during a half-way denitrification reaction according to the present invention. In fig. 9, on the abscissa, the nitrate nitrogen conversion rate (removal rate) and the highest removal average speed (removal rate) of nitrate nitrogen are on the ordinate, wherein the nitrate nitrogen conversion rate c= (N 0-N)/N0, nitrate nitrogen removal average speed v=n/T (N 0: initial concentration of nitrate nitrogen, N: concentration of nitrate nitrogen, T: reaction time) and linear regression equations of different sulfur nitrogen ratios with the nitrate nitrogen removal rate and removal rate are constructed, see formulas (1) to (2).
y1＝0.695×x1+5.607 (1)；
In the formula (1), y1 is the highest removal average speed of nitrate nitrogen, the unit is mg.L -1·h-1, x1 is the sulfur-nitrogen molar ratio, and the correlation coefficient is R 2 = 0.9765.
y2＝-0.704×x2+80.602 (2)；
In the formula (2), y2 is the nitrate nitrogen removal rate, the unit is that x2 is the sulfur nitrogen molar ratio, and the correlation coefficient R 2 =0.9973.
In addition, according to the data of the nitrate nitrogen removal rate and the removal rate under different sulfur-nitrogen ratio conditions, a linear regression equation of the nitrate nitrogen removal rate, the removal rate and the sulfur-nitrogen molar ratio in the reactor is constructed, as shown in fig. 10.
FIG. 10 is a graph of quantitative model of sulfur to nitrogen ratio versus nitrate nitrogen conversion during the course of the half-denitrification reaction of the present invention. In fig. 10, regression analysis is performed on a linear regression equation of the nitrate nitrogen removal rate, the removal rate and the molar ratio of sulfur and nitrogen in the simultaneous reactor, the removal rate and the removal rate are taken as independent variables, the sulfur and nitrogen ratio is taken as a dependent variable to be taken as a binary regression equation, and an Origin software (Excel software can also be used) is adopted to fit and construct a linear regression equation of the nitrate nitrogen removal rate, the removal rate and the molar ratio of sulfur and nitrogen in the simultaneous reactor, as shown in a formula (3).
X＝-1.922×Y1^0.5-1.765×Y2+146.8753 (3)；
Wherein X is a sulfur-nitrogen molar ratio, Y 1 is a maximum removal average rate of nitrate nitrogen, the unit is mg·l -1·h-1,Y2 is a nitrate nitrogen removal rate, the unit is a correlation coefficient R 2 =0.9987.
From the results in FIG. 9, it was found that when the maximum removal rate of nitrate nitrogen was 7.554 mg.L -1·h-1 and the maximum removal rate was 78.630%, the nitrate nitrogen was brought into formula (3), and the sulfur-nitrogen molar ratio was 2.801. From this, it is found that the optimum sulfur-nitrogen ratio is 2.801 on the premise of both the removal rate and the removal rate.
According to the result, in the method, in a culture system of denitrifying bacteria, sulfur-nitrogen-containing wastewater is added, the molar ratio of sulfur to nitrogen in the system is regulated to be 0.675-6:1, the mass ratio of carbon to nitrogen is 1.02-2.04:1, and half-way denitrification reaction is carried out under the anoxic condition, so that the denitrification effect of the denitrifying bacteria can be effectively regulated and controlled, the nitrite nitrogen conversion effect in the half-way denitrification process can be improved, the generation rate and conversion rate of nitrite nitrogen in the system can be effectively controlled, and stable and efficient electron acceptors can be provided for the half-way denitrification-anaerobic ammonia oxidation process according to actual needs, and finally, the efficient and deep denitrification of sewage can be realized.
The above description is only of the preferred embodiment of the present invention, and is not intended to limit the present invention in any way. While the invention has been described in terms of preferred embodiments, it is not intended to be limiting. Any person skilled in the art can make many possible variations and modifications to the technical solution of the present invention or equivalent embodiments using the method and technical solution disclosed above without departing from the spirit and technical solution of the present invention. Therefore, any simple modification, equivalent substitution, equivalent variation and modification of the above embodiments according to the technical substance of the present invention, which do not depart from the technical solution of the present invention, still fall within the scope of the technical solution of the present invention.
Claims (9)
1. The method for improving the nitrite nitrogen conversion effect in the half-way denitrification process is characterized by comprising the following steps of:
s1, inoculating denitrifying bacteria into an activation culture medium, and performing activation culture to obtain an activated microbial inoculum;
S2, inoculating the activated microbial inoculum into a reactor, adding sulfur-nitrogen-containing wastewater, adjusting the molar ratio of sulfur to nitrogen in the reactor to be 0.675-6:1 and the mass ratio of carbon to nitrogen to be 1.02-2.04:1, and performing half-way denitrification reaction under the anoxic condition.
2. The method for improving the effect of nitrite nitrogen conversion in a half-way denitrification process according to claim 1, further comprising the steps of:
s3, according to the sulfur-nitrogen molar ratio in the reactor, combining a linear regression equation of the sulfur-nitrogen molar ratio in the reactor and the highest removal average speed of nitrate nitrogen, and obtaining the highest removal average speed of the nitrate nitrogen in the reaction system; the linear regression equation of the sulfur-nitrogen molar ratio and the nitrate nitrogen removal rate in the reactor is shown as a formula (1);
y1＝0.695×x1+5.607 (1)；
In the formula (1), y1 is the highest removal average speed of nitrate nitrogen, the unit is mg.L -1·h-1, x1 is the sulfur-nitrogen molar ratio, and the correlation coefficient is R 2 = 0.9765.
3. The method for improving the effect of nitrite nitrogen conversion in a half-way denitrification process according to claim 1, further comprising the steps of:
S4, according to the molar ratio of sulfur to nitrogen in the reactor, and in combination with a linear regression equation of the molar ratio of sulfur to nitrogen in the reactor and the nitrate nitrogen removal rate, the nitrate nitrogen removal rate in the reaction system is obtained; the linear regression equation of the sulfur-nitrogen molar ratio and the nitrate nitrogen removal rate in the reactor is shown as a formula (2);
y2＝-0.704×x2+80.602 (2)；
In the formula (2), y2 is the nitrate nitrogen removal rate, the unit is that x2 is the sulfur nitrogen molar ratio, and the correlation coefficient R 2 =0.9973.
4. The method for improving the effect of nitrite nitrogen conversion in a half-way denitrification process according to claim 1, further comprising the steps of:
S5, according to the removal rate and the removal rate of the nitrate nitrogen, combining a linear regression equation of the removal rate and the removal rate of the nitrate nitrogen with the molar ratio of sulfur and nitrogen in the reactor, and obtaining the molar ratio of sulfur and nitrogen in the reaction system; the linear regression equation of the nitrate nitrogen removal rate, the removal rate and the sulfur-nitrogen molar ratio in the reactor is shown in a formula (3);
X＝-1.922×Y1^0.5-1.765×Y2+146.8753 (3)；
Wherein X is a sulfur-nitrogen molar ratio, Y 1 is a maximum removal average rate of nitrate nitrogen, the unit is mg·l -1·h-1,Y2 is a nitrate nitrogen removal rate, the unit is a correlation coefficient R 2 =0.9987.
5. The method for improving nitrite nitrogen conversion effect in a half-way denitrification process according to any one of claims 1 to 4, wherein in step S2, when the molar ratio of sulfur to nitrogen in the reactor is 0.675-2.5:1, the half-way denitrification reaction time is not less than 45 hours; or when the molar ratio of sulfur to nitrogen in the reactor is 2.5-6:1, the time of the half-way denitrification reaction is 5-45 h.
6. A method for improving the effect of nitrite nitrogen conversion in a half-process denitrification process according to any one of claims 1 to 4, wherein in step S2, said sulfur-nitrogen-containing wastewater is at least one of landfill leachate, leather wastewater, and pharmaceutical wastewater; the sulfur-nitrogen-containing wastewater contains nitrate, thiosulfate, carbonate and potassium salt, wherein the concentration of the nitrate in the sulfur-nitrogen-containing wastewater is 0.911g/L, the concentration of the thiosulfate is 1.329 g/L-10.637 g/L, the concentration of the carbonate is 1g/L, the concentration of the potassium salt is 0.636g/L, the nitrate is NaNO 3, the thiosulfate is Na 2S2O3·5H2 O, the carbonate is NaHCO 3, and the potassium salt is KCl.
7. The method for improving nitrite nitrogen conversion effect in a half-way denitrification process according to any one of claims 1 to 4, wherein in step S2, the volume ratio of the activated microbial inoculum to the sulfur-nitrogen-containing wastewater is 0.1-0.15:1, and the anoxic condition is continuous nitrogen introduction.
8. A method for improving the effect of nitrite nitrogen conversion in a half-process denitrification process according to any one of claims 1-4, wherein in step S1, the activating medium comprises the following components: 10.0g/L peptone, 5.0g/L yeast powder, 5.0g/L sodium chloride; the pH value of the activation culture medium is 6.8-7.2.
9. The method for improving nitrite nitrogen converting effect in a half-process denitrification process according to any one of claims 1 to 4, wherein in step S1, the temperature of the activation culture is 25 ℃ to 29 ℃, and the time of the activation culture is 3 days to 5 days.
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