CN118177193A - Nanometer plant immunity-inducing agent seed coating agent and application thereof - Google Patents
Nanometer plant immunity-inducing agent seed coating agent and application thereof Download PDFInfo
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Landscapes
- Pretreatment Of Seeds And Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention provides a nano plant immunity-inducing agent seed coating agent, which is prepared from the following raw materials: plant immunity inducer, film forming agent, wetting agent, dispersing agent, penetrating agent, thickening agent and other auxiliary agents. The plant immunity resistance inducer seed coating agent provided by the invention can form a layer of protective film on seeds, is breathable and permeable, and improves germination rate and yield; meanwhile, the plant immunity inducer can stimulate and activate the molecular immune system in the plant body, improve the disease resistance of the plant, and simultaneously stimulate a series of metabolism regulation and control systems in the plant body, thereby promoting the growth of roots, stems and leaves of the plant, improving the chlorophyll content and finally playing a role in increasing the yield of crops.
Description
Technical Field
The invention relates to the technical field of pesticides, in particular to a nano plant immunity-inducing and resistance-inducing seed coating agent and application thereof.
Background
The seed coating agent is a new pesticide formulation for coating the outside of seeds, and is a seed treatment agent which is processed by a special process together with auxiliaries such as film forming agents, dispersing agents and the like on the basis of some pesticides. After the seeds are treated by the seed coating agent, a layer of agent film is coated on the surfaces of the seeds, so that the health of the seeds can be better ensured for a long time. After the seeds treated by the seed coating agent are sown, part of the agent can be slowly released in the soil, and the other agent can enter the inside of the seeds, so that plant diseases and insect pests can be prevented and controlled for a long time.
The plant immunity inducer can induce plants to generate immune response and activate metabolic regulation, strengthen disease resistance and stress resistance of the plants, promote plant growth and finally achieve the aim of increasing yield. The commonly used plant immunity inducer is sprayed after the seeds germinate, and the effect is not ideal. The reason is that the utilization rate is not always up, and the leaves, the stem bark and the like of the plants can influence the absorption of the plant immune elicitor.
CN104904749a discloses a plant immune elicitor and its application, the immune elicitor comprises the following active ingredients by weight percent: 0.001-0.02% of amino-oligosaccharin and 0.01-0.2% of phosphite; the amino oligosaccharin and phosphite are combined, the mixing proportion of the components of the obtained immunity inducer is reasonable, and the immunity inducer is applied to corn, so that the immunity inducer has good control effect on small spot disease, can improve the yield of corn, and realizes the synchronization of corn powder scattering and spinning.
CN110063364a provides a plant immunity inducer and its application, the effective components of the plant immunity inducer include 2 amino 3,5 dichlorobenzoic acid, sulfachloropyridazine and chitosan. The occurrence of the immunological blue mold of the picked citrus can be induced, the damage effect of the blue mold to the quality of the picked citrus is effectively inhibited, and the preservative period of the picked citrus is prolonged; the plant immunity inducer is nontoxic and pollution-free, can directly act on the harvested citrus, and has no pesticide residue; can also effectively prevent and treat mango anthracnose after picking.
In order to solve the problem, the invention mixes the plant immunity inducing and resisting agent and seed coating agent for the first time, and the plant immunity inducing and resisting agent is treated at the beginning of the seed stage, thus greatly increasing the plant immunity inducing and resisting effect.
Disclosure of Invention
The invention provides a nano plant immunity-inducing agent seed coating agent, which is prepared from the following raw materials: plant immunity inducer, film forming agent, wetting agent, dispersing agent, penetrating agent, thickening agent and other auxiliary agents.
Preferably, the plant immune elicitor is a carbohydrate plant immune elicitor.
Preferably, the saccharide plant immunity inducer is selected from one of oligosaccharin, amino oligosaccharin, lentinan and chitosan.
Preferably, the film forming agent is selected from one or more of xanthan gum, carboxypropyl cellulose, polyvinyl alcohol, chitosan and polyethylene glycol.
Preferably, the wetting agent is selected from sodium lauryl sulfate, sodium dodecylbenzene sulfonate, and sulfosuccinate.
Preferably, the dispersant is ethanol.
Preferably, the penetrating agent is a charred fiber.
Preferably, the thickener is magnesium aluminum silicate.
Preferably, the further auxiliary agent is selected from disintegrants or binders.
The invention also provides application of the nano plant immunity-inducing agent seed coating agent in rice.
By adopting the technical scheme, the invention has the following beneficial effects:
The plant immunity resistance inducer seed coating agent provided by the invention can form a layer of protective film on seeds, is breathable and permeable, and improves germination rate and yield; meanwhile, the plant immunity inducer can stimulate and activate the molecular immune system in the plant body, improve the disease resistance of the plant, and simultaneously stimulate a series of metabolism regulation and control systems in the plant body, thereby promoting the growth of roots, stems and leaves of the plant, improving the chlorophyll content and finally playing a role in increasing the yield of crops.
Detailed Description
The following description of the present invention will be made clearly and fully, and it is apparent that the embodiments described are some, but not all, of the embodiments of the present invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
The invention provides a nano plant immunity-inducing agent seed coating agent, which is prepared from the following raw materials: plant immunity inducer, film forming agent, wetting agent, dispersing agent, penetrating agent, thickening agent and other auxiliary agents.
Plant immunity inducer
The plant immunity inducer can be selected from exogenous proteins, saccharides, organic acids and the like, and the plant immunity inducer is the saccharide plant immunity inducer in the invention.
Preferably, the saccharide plant immunity inducer is selected from one of oligosaccharin, amino oligosaccharin, lentinan and chitosan.
More preferably, the saccharide plant immunity-inducing agent is a compound of amino-oligosaccharin and chitosan, and the compound ratio is 2:1.
Film forming agent
The film forming agent has the main function of ensuring that a film is formed on the surface of the seeds, and forming a protective and stimulated film layer for the seeds.
In the invention, the film forming agent is one or more selected from xanthan gum, carboxypropyl cellulose, polyvinyl alcohol, chitosan and polyethylene glycol.
Preferably, the film forming agent is a mixture of polyvinyl alcohol and chitosan, and the mixing mass ratio is 1:1.
As a preferred mode of the present invention, the film forming agent further comprises an N, N-dimethylethylaldehyde alkylamine complex solution; its cas number is: 124330-23-0. More preferably, the film former further comprises nanosilica.
Wetting agent
In the invention, the wetting agent is selected from sodium lauryl sulfate, sodium dodecyl benzene sulfonate and sulfosuccinate; sodium lauryl sulfate is more preferred.
Dispersing agent
The dispersing agent in the invention is selected to be a liquid dispersing agent at normal temperature, and ethanol is preferable.
Penetrant
In the invention, the penetrating agent is carbonized fiber.
Thickening agent
In the invention, the thickener is magnesium aluminum silicate.
Other auxiliary agents
In the present invention, the other auxiliary agent is selected from a disintegrating agent or a binder.
Wherein the disintegrating agent is cross-linked polyvinylpyrrolidone; the binder is sodium alginate.
The preparation process comprises the following steps:
The invention provides a preparation method of the seed coating agent, which comprises the following steps: stirring and mixing the plant immunity inducer, the penetrating agent, the thickening agent and other auxiliary agents, adding the film forming agent into the mixture for ball milling and mixing, and then spraying and mixing the mixed liquid of the dispersing agent and the wetting agent in the stirring process.
The invention also provides application of the nano plant immunity-inducing agent seed coating agent in rice.
Explanation of the mechanism: the nano plant immunity induction agent seed coating agent provided by the application combines the plant immunity induction agent and the seed coating agent for the first time, so that not only can a better coating effect and a plant immunity induction effect be realized; meanwhile, the present inventors have found that since the plant immunity inducer needs to stimulate the surface protein, it is often required to have an excellent permeation effect; and nano-materials or metals are also considered as auxiliary materials. Research shows that when metal ions are adopted as plant immunity elicitor for treatment, the concentration requirement is often too high; too low a concentration does not achieve the osmotic effect, and too high a concentration may cause drought stress higher than salinity stress, and the germination of seeds is completely inhibited, not to mention the immune induction effect. According to the application, the N, N-dimethylethylaldehyde amine complex solution and the nano silicon dioxide material are matched, so that a good drought stress effect can be generated; meanwhile, the requirement on the concentration range is not very strict; the reason is probably because the N, N-dimethylethylaldehyde amine complex solution can react with chitosan in the application, so that crosslinking is realized, a better film layer is formed, and the seed germination rate is high; and simultaneously, the generated superfluous Al ions can act with nano silicon dioxide to generate better plant immunity induction effect. Meanwhile, the formula adopted by the application does not need to be additionally added with an antifreeze agent, and the preparation method provided by the application is matched, so that the seed coating agent provided by the application has the advantages of uniform coating, uniform film layer, ventilation, high germination rate, easiness in storage and good product stability.
The invention is further illustrated with reference to specific embodiments.
Example 1: the embodiment provides a nano plant immunity-inducing agent seed coating agent, which is prepared from the following raw materials in parts by weight: 20 parts of plant immunity inducer, 1.5 parts of film forming agent, 4 parts of wetting agent, 2 parts of dispersing agent, 1 part of penetrating agent, 0.5 part of thickening agent, 0.03 part of disintegrating agent and 0.5 part of binding agent.
Wherein the plant immunity inducer is the compound of amino-oligosaccharin and chitosan, and the compound ratio is 2:1. The film forming agent is polyvinyl alcohol, chitosan, N-dimethyl ethyl aldehyde alkylamine complex solution and nano silicon dioxide, and the mass ratio of the polyvinyl alcohol to the chitosan to the N, N-dimethyl ethyl aldehyde alkylamine complex solution to the nano silicon dioxide is 1:1:0.5:0.05; the wetting agent is sodium lauryl sulfate; the dispersing agent is ethanol; the penetrating agent is carbonized fiber; the thickener is magnesium aluminum silicate; the disintegrating agent is cross-linked polyvinylpyrrolidone; the binder is sodium alginate.
The nano silicon dioxide is purchased from Jiangsu Tiansu New Material Co., ltd, and the model is TSP-F90.
The embodiment provides a preparation method of the seed coating agent: mixing plant immunity inducer, penetrating agent, thickener and other auxiliary agent in proportion, adding film forming agent into the mixture, ball milling, spraying and mixing the mixed liquid of dispersant and wetting agent, and granulating.
Example 2: the difference between the present embodiment and embodiment 1 is that the film forming agent is polyvinyl alcohol, chitosan, N-dimethylethylaldehyde alkylamine complex solution, and nano silicon dioxide according to the mass ratio of 1:2:0.5:0.05.
Example 3: this example differs from example 1 in that the raw materials for its preparation, in parts by weight, comprise: 20 parts of plant immunity inducer, 2 parts of film forming agent, 4 parts of wetting agent, 2 parts of dispersing agent, 1 part of penetrating agent, 0.5 part of thickening agent, 0.03 part of disintegrating agent and 0.5 part of binding agent.
Example 4: this example differs from example 1 in that the film former does not comprise an N, N-dimethylethylaldehyde alkylamine complex solution.
Example 5: this example differs from example 1 in that the solution of the N, N-dimethylethylaldehyde alkylamine complex in the film former is replaced with glyoxal.
Example 6: this example differs from example 1 in that the film former does not contain nanosilica.
Example 7: the difference between the present embodiment and embodiment 1 is that the film forming agent is polyvinyl alcohol, chitosan, N-dimethylethylaldehyde alkylamine complex solution, and nano silicon dioxide according to the mass ratio of 1:1:0.7:0.05.
Example 8: this example differs from example 1 in that the dispersant is sodium dodecylbenzenesulfonate.
Example 9: this example differs from example 1 in that the penetrant is sodium isooctyl sulfosuccinate of the charred fiber.
Example 10: this example differs from example 1 in that the thickener is xanthan gum.
Performance testing
1. Test 1: and (3) mixing the seed coating agent prepared in the embodiment with water to prepare 10wt% of the seed coating agent, mixing the seed coating agent with rice seeds according to the mass ratio of 1:10, fully shaking until the seed coating agent and the water are uniformly mixed, and airing to finish the coating of the rice seeds.
Germination experiment
Randomly taking 100 coated rice seeds, placing the coated rice seeds on a paper bed of a germination box, alternately carrying out 12-hour illumination and 12-hour dark culture every day, keeping the temperature at 20 ℃, spraying water at proper amount every day at regular time to keep the germination paper moist, starting counting the germination rate (the length of radicle exceeds the length of the seeds to be regarded as germination) at the fourth day of the experiment, and recording the germination rate and the mildew rate when two leaves grow out, namely the germination is ended; wherein the control group was prepared by using uncoated rice seeds as a control.
Table 1 test results of test 1
Examples | Germination percentage, percent | Mould generation rate, percent |
Example 1 | 96 | 0 |
Example 2 | 91 | 0 |
Example 3 | 94 | 0 |
Example 4 | 73 | 0 |
Example 5 | 69 | 5 |
Example 6 | 63 | 0 |
Example 7 | 90 | 0 |
Example 8 | 85 | 0 |
Example 9 | 81 | 0 |
Example 10 | 76 | 0 |
Control group | 53% | 14% |
2. Test 2: the test crops are rice, the rice is planted in a Jiushan farm in Shanghai city, and is sowed in the spring in 2021, 3 and 20 days, and harvested in a unified manner in 7 and 21 days. The water and fertilizer cultivation conditions of the test cells are consistent.
The test set up consisted of 10 treatment groups [ examples 1-10, respectively, of test 1 above ].
The rice treated in the above examples and comparative examples was examined for occurrence of seedling rot, and the yield was measured, and the results are shown in tables 2 and 3.
TABLE 2 plant immune elicitor treatment for the occurrence of seedling rot in rice plants
The occurrence conditions are as follows: almost none, a small fraction, half and a large fraction are present.
TABLE 3 Effect of plant immune elicitor treatment on Rice yield
Examples | Average mu yield (kg) | Comparison of control (. + -.) |
Example 1 | 881.6 | 15.4 |
Example 2 | 867.9 | 13.6 |
Example 3 | 877.8 | 14.9 |
Example 4 | 825.1 | 8.2 |
Example 5 | 812.4 | 6.4 |
Example 6 | 867.1 | 13.5 |
Example 7 | 857.2 | 12.2 |
Example 8 | 847.3 | 10.9 |
Example 9 | 839.6 | 9.9 |
Example 10 | 836.9 | 9.5 |
3. Test 3: the rice prepared in the example 1 is used as seeds to be sown in spring of the next year, compared with the rice seeds purchased in the market, the rice obtained in the example 1 is used as seeds, no additional coating treatment is carried out, the germination rate is close to 93%, and the average mu yield is 859.1kg; commercial 56% and average mu yield 713.2kg.
4. Test 4: the seed coating agents prepared in examples 1 to 10 were placed at-3℃for 24 hours to check whether caking occurred.
Examples | With or without caking |
Example 1 | Without any means for |
Example 2 | Without any means for |
Example 3 | Without any means for |
Example 4 | Without any means for |
Example 5 | Without any means for |
Example 6 | Without any means for |
Example 7 | Without any means for |
Example 8 | Has the following components |
Example 9 | Has the following components |
Example 10 | Has the following components |
Finally, it should be noted that: the above embodiments are only for illustrating the technical solution of the present invention, and not for limiting the same; although the invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some or all of the technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit of the invention.
Claims (10)
1. The nanometer plant immunity inducing and resisting seed coating agent is characterized by comprising the following raw materials: plant immunity inducer, film forming agent, wetting agent, dispersing agent, penetrating agent, thickening agent and other auxiliary agents.
2. The nano-plant immunity-inducing agent seed coating agent according to claim 1, wherein the plant immunity-inducing agent is a saccharide plant immunity-inducing agent.
3. The nano plant immunity-inducing agent seed coating agent according to claim 2, wherein the saccharide plant immunity-inducing agent is selected from one of oligosaccharins, amino oligosaccharins, lentinan, chitosan species.
4. The nano plant immunity-inducing agent seed coating agent according to claim 1, wherein the film forming agent is selected from one or more of xanthan gum, carboxypropyl cellulose, polyvinyl alcohol, chitosan, polyethylene glycol.
5. The nano-plant immune elicitor seed coating according to claim 1, wherein the wetting agent is selected from sodium lauryl sulfate, sodium dodecylbenzenesulfonate, sulfosuccinate.
6. The nano-plant immunity-inducing agent seed coating agent according to claim 1, wherein the dispersing agent is ethanol.
7. The nano-plant immunity-inducing agent seed coating agent according to claim 1, wherein the penetrating agent is a carbonized fiber.
8. The nano-plant immunity-inducing agent seed coating agent according to claim 1, wherein the thickener is magnesium aluminum silicate.
9. The nano-plant immunity-inducing agent seed coating agent according to claim 1, wherein the other auxiliary agent is selected from a disintegrant or a binder.
10. Use of a nano-plant immunity-inducing agent seed coating according to any one of claims 1-9 in rice.
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