CN118141835A - Antibacterial liquid - Google Patents
Antibacterial liquid Download PDFInfo
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- CN118141835A CN118141835A CN202410222915.0A CN202410222915A CN118141835A CN 118141835 A CN118141835 A CN 118141835A CN 202410222915 A CN202410222915 A CN 202410222915A CN 118141835 A CN118141835 A CN 118141835A
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- Prior art keywords
- colorless transparent
- liquid
- transparent liquid
- test
- bacteriostatic
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- 239000007788 liquid Substances 0.000 title claims abstract description 55
- 230000000844 anti-bacterial effect Effects 0.000 title description 32
- 230000003385 bacteriostatic effect Effects 0.000 claims abstract description 11
- 229910021642 ultra pure water Inorganic materials 0.000 claims abstract description 11
- 239000012498 ultrapure water Substances 0.000 claims abstract description 11
- 229910052709 silver Inorganic materials 0.000 claims abstract description 9
- 239000004332 silver Substances 0.000 claims abstract description 9
- -1 silver ions Chemical class 0.000 claims abstract description 9
- 241000222122 Candida albicans Species 0.000 claims abstract description 8
- 241000191967 Staphylococcus aureus Species 0.000 claims abstract description 8
- 229940095731 candida albicans Drugs 0.000 claims abstract description 8
- 241000588724 Escherichia coli Species 0.000 claims abstract description 7
- 230000000813 microbial effect Effects 0.000 claims abstract description 3
- 241000894006 Bacteria Species 0.000 claims description 5
- 230000002401 inhibitory effect Effects 0.000 claims 1
- 238000004140 cleaning Methods 0.000 abstract description 2
- 210000000214 mouth Anatomy 0.000 abstract description 2
- 238000012360 testing method Methods 0.000 description 36
- 241000700605 Viruses Species 0.000 description 11
- 230000002779 inactivation Effects 0.000 description 11
- 241000712461 unidentified influenza virus Species 0.000 description 10
- 241000590002 Helicobacter pylori Species 0.000 description 9
- 229940037467 helicobacter pylori Drugs 0.000 description 9
- 239000000523 sample Substances 0.000 description 9
- 239000000725 suspension Substances 0.000 description 9
- 238000010790 dilution Methods 0.000 description 7
- 239000012895 dilution Substances 0.000 description 7
- 238000001514 detection method Methods 0.000 description 6
- 241000701806 Human papillomavirus Species 0.000 description 5
- 230000001580 bacterial effect Effects 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 4
- 210000003837 chick embryo Anatomy 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 239000001963 growth medium Substances 0.000 description 4
- 238000006386 neutralization reaction Methods 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 3
- 241001112090 Pseudovirus Species 0.000 description 3
- 238000003556 assay Methods 0.000 description 3
- 229940098773 bovine serum albumin Drugs 0.000 description 3
- 238000013095 identification testing Methods 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 241000233866 Fungi Species 0.000 description 2
- 239000000159 acid neutralizing agent Substances 0.000 description 2
- 244000052616 bacterial pathogen Species 0.000 description 2
- 229910002092 carbon dioxide Inorganic materials 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 239000013068 control sample Substances 0.000 description 2
- 230000002500 effect on skin Effects 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000035931 haemagglutination Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 239000013642 negative control Substances 0.000 description 2
- 230000003472 neutralizing effect Effects 0.000 description 2
- 231100000989 no adverse effect Toxicity 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 208000035143 Bacterial infection Diseases 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 1
- 241000341655 Human papillomavirus type 16 Species 0.000 description 1
- 241000712431 Influenza A virus Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 208000002606 Paramyxoviridae Infections Diseases 0.000 description 1
- 206010040880 Skin irritation Diseases 0.000 description 1
- 208000022362 bacterial infectious disease Diseases 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 239000007382 columbia agar Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000000312 effect on influenza Effects 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 239000008055 phosphate buffer solution Substances 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 206010040872 skin infection Diseases 0.000 description 1
- 230000036556 skin irritation Effects 0.000 description 1
- 231100000475 skin irritation Toxicity 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a bacteriostatic liquid in the technical field of bacteriostatic liquids, which comprises the following components in percentage by mass: 0.4-0.7 ug of electrolytic silver ions and 8-14ml of ultrapure water are mixed into colorless transparent liquid, the PH value of the colorless transparent liquid is 5.6-6.5, the colorless transparent liquid can inhibit candida albicans and staphylococcus aureus of escherichia coli, and the microbial index of the colorless transparent liquid accords with the sanitary standard GB15979-2002 of disposable sanitary products. The invention has the advantages that: the embodiment of the invention can be suitable for sanitary cleaning and bacteriostasis of skin and oral cavity.
Description
Technical Field
The invention relates to the technical field of antibacterial liquid, in particular to antibacterial liquid.
Background
Skin is the first immune barrier of the human body, and is often affected by various microorganisms such as bacteria, fungi, mold, viruses and the like along with human-to-object, animal and human-to-human contact in daily life and work. Especially staphylococcus aureus, candida albicans, colibacillus and other bacteria and some fungi can cause skin irritation and accelerate skin infection, and even can cause pathological influence on fragile skin or susceptibility skin.
The existing antibacterial liquid has complex components and poor antibacterial effect.
Disclosure of Invention
In order to solve the problems, the invention provides a bacteriostatic liquid which can be suitable for sanitary cleaning and bacteriostasis of skin and oral cavities.
In order to achieve the above purpose, the technical scheme provided by the invention is as follows:
the antibacterial liquid comprises the following components in percentage by mass: 0.4-0.7 ug of electrolytic silver ions and 8-14ml of ultrapure water are mixed to prepare colorless transparent liquid.
Further, the ratio of the electrolytic silver ions to the ultrapure water is 1:20.
Further, the pH value of the colorless transparent liquid is 5.6-6.5.
Further, the colorless transparent liquid can inhibit candida albicans and staphylococcus aureus of escherichia coli.
Furthermore, the microorganism index of the colorless transparent liquid accords with the sanitary standard GB15979-2002 of disposable sanitary products.
The beneficial effects obtained by the invention by adopting the structure are as follows:
The antibacterial liquid is colorless and transparent liquid, is prepared by mixing electrolytic silver ions and ultrapure water, has no adverse effect on skin, has good skin-friendly effect, can effectively inhibit escherichia coli, candida albicans and staphylococcus aureus, has high antibacterial rate, and has antibacterial effect on other various germs such as influenza virus H1N1, helicobacter pylori, human papilloma virus (HPV 16) and the like.
Drawings
FIG. 1 shows the result of the neutralizer identification test of the present invention.
FIG. 2 is a schematic diagram showing the inactivation effect of the parainfluenza virus H1N1 according to the present invention.
FIG. 3 is a schematic view showing the bacteriostatic effect against helicobacter pylori according to the invention.
FIG. 4 shows the results of an assay for human papillomavirus (HPV 16) inactivation according to the present invention.
Detailed Description
The antibacterial liquid comprises the following components in percentage by mass: 0.4-0.7 ug of electrolytic silver ions and 8-14ml of ultrapure water are mixed to form colorless transparent liquid, the ratio of the electrolytic silver ions to the ultrapure water is 1:20, and the PH value of the colorless transparent liquid is 5.6-6.5.
The colorless transparent liquid can inhibit escherichia coli, candida albicans and staphylococcus aureus, the bacteriostasis rate of the colorless transparent liquid on the escherichia coli, candida albicans and staphylococcus aureus is more than 90%, and the microbial index of the colorless transparent liquid accords with the disposable hygienic product hygienic standard GB 15979-2002.
Example 1: testing the inactivation detection experiment of the colorless transparent liquid on the influenza virus H1N1
1. Equipment and method for manufacturing the same
1. The test strain was influenza virus H1N1 (Influenza A Virus H N1, ATCC VR-1469).
2. White shell chick embryo of 9-11 d age, SPF grade.
3. Colorless transparent antibacterial liquid.
4. Neutralizing agent: D/E neutralization broth.
5. 96-Well hemagglutination titer assay plate.
6. Organic interfering substance 3% Bovine Serum Albumin (BSA).
7. Humidifying incubator, thermostat, centrifuge, sterile injector, biosafety cabinet, sterile equipment, etc.
2. Method of
1. The detection is based on the 2.1.1.10 th item of disinfection technical Specification (2002 edition).
2. The preparation of virus suspension, which is to test influenza virus H1N1 with hemagglutination titer more than or equal to 1:640, and to dilute the suspension with 3% bovine serum albumin organic interference substance pair by times, and to keep the temperature at 20 ℃ for standby.
3. The neutralization agent identification test is that colorless transparent antibacterial liquid is used for 15.0min, ultrapure water is used for proper dilution, physiological saline can not be used for dilution, and 0.2mL of each dilution sampling liquid is inoculated with 4 chick embryo allantoic cavities. The test temperature was constant at 20℃and the test was repeated 3 times.
4. And (3) virus inactivation test, namely inoculating 4 chick embryo allantoic cavities with colorless transparent antibacterial liquid for 15.0min, and using ultrapure water as proper dilution, wherein 0.2mL of each dilution sample liquid is used for inoculating. The test temperature was constant at 20 ℃. The test was repeated 3 times.
5. The ambient temperature is 21.6-23.0 ℃; the relative humidity is 43-50%.
3. Results
1. Neutralization agent identification test
The neutralizing agent of the colorless transparent antibacterial liquid has been tested repeatedly for 3 times under the condition of constant temperature of 20deg.C, and the average titer of the virus in group 1 is 4.50, the average titer of the virus in group 2 is 5.19, the average titer of the virus in group 3, group 4 and group 5 is 6.11, 6.24 and 6.38, respectively, and the group 6 is negative control group (see figure 1)
2. Inactivation effect on influenza virus
After 3 repeated tests, colorless transparent antibacterial liquid is applied for 15.0min under the constant temperature test condition of 20 ℃, and the average inactivation logarithmic value of influenza virus H1N1 in suspension is 1.20 (see figure 2).
4. Conclusion(s)
1. Through 3 repeated tests, the D/E neutralization broth neutralizer can effectively neutralize the residual effect of the colorless transparent antibacterial liquid on influenza virus H1N1 under the constant temperature test condition of 20 ℃, and the neutralization product of the neutralizer and the neutralization broth has no influence on the growth of influenza virus H1N1 and chick embryos.
2. Through 3 repeated tests, colorless transparent antibacterial liquid is applied under the constant temperature test condition of 20 ℃ for 15.0min, and the average inactivation logarithmic value of influenza virus H1N1 in suspension is 1.20 (namely, the average inactivation rate is 93.69%).
Example 2: device for testing first and second quantitative antibacterial tests of helicobacter pylori carrier soaking by colorless transparent liquid
1. The test strain helicobacter pylori (ATCC 43504), passage 3.
2. Colorless transparent antibacterial liquid.
3. The diluted solution is phosphate buffer solution (PBS, 0.03M,pH 7.2).
4. Test vehicle a piece of plain cotton cloth (10 mm. Times.10 mm) was defatted.
5. Culture medium helicobacter pylori culture medium (brain heart infusion powder, columbia agar and distilled water).
6. Biological safety cabinet, constant temperature incubator, thermostat, vortex oscillator, aseptic equipment and electronic timer, etc.
2. Method of
1. The detection basis is WS/T650-2019, 5.1.2 of antibacterial and bacteriostatic effect evaluation method.
2. The preparation of the bacterial infection carrier comprises the steps of taking a fresh inclined plane culture (24 h) of the test bacteria, washing the fresh inclined plane culture with PBS, and preparing a bacterial suspension. Absorbing 10uL of bacterial suspension, dripping the bacterial suspension on a sterile carrier, uniformly coating the bacterial suspension, and drying the bacterial suspension for later use.
3. The test method comprises weighing sample according to the amount of 5 g/piece, placing in a sterile plate, placing in 20deg.C water bath for 5min, immersing in sample with sterile forceps, immediately timing to 15.0min, taking out sample piece with sterile forceps, adding into test tube containing 5mLPBS solution, thoroughly mixing, washing test bacteria, moderately diluting with PBS, absorbing 0.1mL sample liquid, and smearing on helicobacter pylori culture medium as test group. And (3) immersing 2 pieces of bacteria-infected carriers in 10g of control sample which does not contain antibacterial components and is the same material as the test sample for parallel test, and taking the control sample as positive control. The aspiration test uses 0.1mL of PBS, smeared with plates, in duplicate, as a negative control group. The test temperature was constant at 20 ℃. The test was repeated 3 times and the bacteriostatic rate was calculated.
4. The ambient temperature is 20.5-21.0 ℃; the relative humidity is 47-48%.
3. Results
After 3 repeated tests, colorless transparent antibacterial liquid is applied under the constant temperature test condition of 20 ℃ for 15.0min, and the antibacterial rate on helicobacter pylori is 94.21% (see figure 3).
4. Conclusion(s)
Through 3 repeated tests, the colorless transparent antibacterial liquid is applied under the constant temperature test condition of 20 ℃ for 15.0min, and the antibacterial rate of helicobacter pylori is more than 90%, so that the antibacterial effect is strong. Meets the regulations of WS/T650-2019 'antibacterial and bacteriostatic effect evaluation method'.
Example 3: testing the colorless transparent liquid for human papillomavirus (HPV 16) inactivation detection experiment
1. Equipment and method for manufacturing the same
1. Sample information: the colorless transparent antibacterial liquid is used as the test object as the original sample.
2. Virus name: HPV pseudovirus (HPV 16-GFP); the source is as follows: beijing Yun Ling organism; lot number: 20220923.
3. Medium name: DMEM/2246299, FBS/2500251P.
4. Instrument apparatus: biosafety cabinet BSC-1604 IIB 2 (WPE-RH 0313), carbon dioxide incubator BPN-CRH (WPE-RH 0274), inverted fluorescence microscope DMi8 (WPE-TW 0021).
2. Method of
1. The test basis is as follows: disinfection technical Specification (2002 edition).
2. Detection environment: temperature: 24.9 ℃, humidity: 57% RH.
3. The experimental steps are as follows:
(1) 293FT cells are inoculated into a 96-well cell culture plate, and the cells are adhered for standby.
(2) Mu.L of HPV16 type pseudovirus liquid is taken, 90 mu.L of test substance or 90 mu.L of PBS is added, stirred and mixed uniformly, incubated for 15 minutes at room temperature, then 900 mu.L of culture medium is added for dilution, and 10 times of gradient dilution is carried out again. 100. Mu.L of the gradient diluted virus solution was added to the cells, and the residual virus titer was determined. 4 well assays were set up for each concentration gradient and incubated in a CO2 incubator at 37 ℃. After 72h of incubation, the number of fluorescent cells was counted using a fluorescence microscope and FFU (fluorescence forming unit) of the virus was calculated.
3. Results
The test results of the colorless transparent liquid on HPV type 16 pseudovirus inactivation are shown in FIG. 4.
4. Conclusion(s)
Through detection, the inactivation logarithmic value of the human papilloma virus (HPV 16) of the sample is more than 4.00, which meets the requirements of disinfection technical Specification (2002 edition).
To sum up: the antibacterial liquid is colorless and transparent liquid, is prepared by mixing electrolytic silver ions and ultrapure water, has no adverse effect on skin, has good skin-friendly effect, can effectively inhibit escherichia coli, candida albicans and staphylococcus aureus, has high antibacterial rate, and has antibacterial effect on other various germs such as influenza virus H1N1, helicobacter pylori, human papilloma virus (HPV 16) and the like.
The invention and its embodiments have been described above with no limitation, and the actual construction is not limited to the embodiments of the invention as shown in the drawings. In summary, if one of ordinary skill in the art is informed by this disclosure, a structural manner and an embodiment similar to the technical solution should not be creatively devised without departing from the gist of the present invention.
Claims (5)
1. A bacteriostatic liquid, which is characterized in that: comprises the following components in percentage by mass: 0.4-0.7 ug of electrolytic silver ions and 8-14ml of ultrapure water are mixed to prepare colorless transparent liquid.
2. A bacteriostatic liquid according to claim 1, characterized in that: the ratio of the electrolytic silver ions to the ultrapure water is 1:20.
3. A bacteriostatic liquid according to claim 2, characterized in that: the PH value of the colorless transparent liquid is 5.6-6.5.
4. A bacteriostatic liquid according to claim 3, characterized in that: the colorless transparent liquid can inhibit candida albicans and staphylococcus aureus of escherichia coli.
5. The bacteria inhibiting liquid of claim 4, wherein: the microbial index of the colorless transparent liquid accords with the sanitary standard GB15979-2002 of disposable sanitary products.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202410222915.0A CN118141835A (en) | 2024-02-28 | 2024-02-28 | Antibacterial liquid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202410222915.0A CN118141835A (en) | 2024-02-28 | 2024-02-28 | Antibacterial liquid |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN118141835A true CN118141835A (en) | 2024-06-07 |
Family
ID=91287811
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202410222915.0A Pending CN118141835A (en) | 2024-02-28 | 2024-02-28 | Antibacterial liquid |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN118141835A (en) |
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- 2024-02-28 CN CN202410222915.0A patent/CN118141835A/en active Pending
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