CN100488471C - Nano antibiotic antiviral condom - Google Patents
Nano antibiotic antiviral condom Download PDFInfo
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- CN100488471C CN100488471C CNB2006100366600A CN200610036660A CN100488471C CN 100488471 C CN100488471 C CN 100488471C CN B2006100366600 A CNB2006100366600 A CN B2006100366600A CN 200610036660 A CN200610036660 A CN 200610036660A CN 100488471 C CN100488471 C CN 100488471C
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- condom
- interferon
- antiviral
- antibacterial
- polyurethane
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Abstract
The invention discloses a nanometer graded antibiotic antiviral condom, which is characterized by the following: grafting interferon on the condom, improving sterilizing activity from 2.1% to 6.3%.
Description
Technical field
The present invention relates to a kind of condom, specifically, relate to a kind of nano antibiotic antiviral condom.
Background technology
Condom is to use the most general contraceptive device in the world, the annual number that consumes is hundreds of millions of, and condom is to not injury of human body, other contraceptive devices that compare have much more very advantages, since eighties of last century since the nineties, a kind of novel polyurethane condom is developed, the advantage that not only has rubber condoms, close such as slippage rate and breakage rate, and be applicable to crowd to the rubber sensitivity, moreover, it is thinner that polyurethane condom can be made, and gives better sensation.
Cervicitis is a kind of gynecological common disease, mainly be divided into gonorrhea type cervicitis and non-gonorrhea cervicitis, gonorrhea type cervicitis is mainly by gonorrhea diplococcus, staphylococcus aureus and Hemolytic streptococcus cause, but not the gonorrhea cervicitis mainly causes the interferon therapy cervicitis mainly by kill virus by virus, and its mechanism is quite ripe.The common minimizing sexual life in various degree of cervicitis patient, and because general condom effect only limits to contraception and shielding virus, and and the antibiotic targetedly and antiviral of impotentia, so if a kind of successful development of strong antibacterial and antiviral condom is arranged, not only can untie cervicitis patient's hearty cord, also more effectively cooperative drug is prevented and treated sexually transmitted disease (STD), the infection of acquired immune deficiency syndrome (AIDS).
IFN is under inducer and some effect of cytokines, and the histone matter by the cytogene coding produces has high activity and multiple function.The virus-free specificity of IFN, a kind of IFN of virus induction is also effective to its virus, but species specificity is arranged, and the IFN that mice produces is invalid in human body.The interference of known person have 3 kinds: IFN-α is a LeIF, and IFN-β is the fibroblast interferon, and plain (a kind of cytokine that the T lymphocyte produces) done in the i.e. immunity of IFN-γ; The first two kind I type, back one kind II type.The gene of coding I type IFN is positioned on human the 9th pair of chromosome, and the gene of coding II type IFN is positioned on the 12nd pair of chromosome.Interferon at first acts on the interferon receptor 2 system on the cell membrane of contiguous uninfection, and the binding site that this system is made up of ganglioside and one may be made up of the activation site that glycoprotein is formed.Existing known I type interferon receptors gene is on human chromosome G21 is long-armed, and II type interferon receptors gene is positioned on the 6th pair of chromosome, and this belongs to specificity with regard to having determined IFN to have certain journey.After IFN and receptors bind, produce a kind of special factor, antiviral protein (AVP) gene is removed suppressed, transcribe and translate AVP, mainly be protein kinase, 2 ＇-5 ＇ A synzyme, phosphodiesterase, these enzymes have substantial connection with the performance antiviral activity.Wherein two kinds of enzymes must activate through bifilar RNA and ATP, and one be protein kinase, and the initiation factors 2 (eIF-2) that can make synthetic protein after the activation is inactivation with phosphorylation, and Profilin matter is synthesized; Another kind is 2 ＇-5 a ＇ A synzyme, and activation back catalysis Synthetic 2 ＇-5 ＇ oligoadenylate activates potential Cobra venom endonuclease again, makes the virus mRNA degraded, and it is synthetic to suppress virus protein.In addition, the phosphodiesterase 2 ＇-5 ＇ A that can degrade can remove the CCA end of tRNA again, and Profilin is synthetic.
Interferon is a kind of medicine of broad-spectrum antibacterial antiviral, if there is a kind of condom can not only possess the advantage of common condom, but also the effect of treatment can anti-bacteria and anti-virus be arranged, and such condom will be a breakthrough of contraception armarium.
Summary of the invention
The objective of the invention is to overcome the deficiency that condom exists, provide a kind of can be antibiotic again can antiviral condom, be applied to prevention and treat various gynaecopathias.
Another object of the present invention provides the preparation method of above-mentioned condom.
To achieve these goals, antibiotic antiviral condom of the present invention is fixed on the antibacterial and antiviral drug grafting on the condom.The condom material can be various macromolecular materials, and preferred condom material is a polyurethane.
Above-mentioned antibacterial and antiviral drug can be various antibiotic and medicines antiviral effect of playing.For example has the antibiotic antineoplastic interferon of broad spectrum antiphlogistic disease.
The present invention adopts the photofixation method that the bacteriostatic grafting is fixed on the macromolecular material of condom.Earlier interferon is added in the solution that contains the inferior amide of N-succinum the ice bath stirring reaction; Spread upon the condom outer surface then, under ultraviolet or 300～800nm visible light, shine.Utilize the photochemical fixation method will have the component of special properties or the method that biomolecule is coupled to material surface, it can block copolymerization, grafting improves the surface character of material own but does not change material body character, reduce unordered crosslinked that the purpose coating reagent produces on the medical macromolecular materials surface, and improve the biocompatibility and the blood compatibility of polyurethane material.
Its grafting principle is suc as formula shown in (I), (II):
Photolytic activity IFN[is connected to IFN on the hot active group of the inferior amide of N-(4-azidobenzoic acid base) succinum by azido fracture] in fragrant azido group through the UV radiation excitation, irreversible photolysis takes place, azido is decomposed into nitrogen and the high nitrence intermedium of active-energy, this intermedium is opened hydrocarbon key in the macromolecule, nitrogen and carbon generation necleophilic reaction, and hydrogen that seizes and carbon atom formation secondary amine, the result makes molecules of interest (IFN) receive on the polyurethane surface with covalent bond.
Compared with prior art, the present invention has following beneficial effect: the present invention is grafted on interferon on the condom, and this has better bactericidal effect than interferon is spread upon on condom.After the interferon grafting was fixed on condom, the bactericidal activity in hour brought up to 6.3% by 2.1% of free interferon, demonstrates 3 times bactericidal activity.Thereby give condom this important birth control instrument more function, even assistance treatment gynecological inflammation, and gynecological inflammation comprises the cause of disease complexity of cervicitis, think relevant in the past with physicochemical irritation, damage or antibacterial, chlamydia, protozoan infection, a large amount of in recent years clinical research datas find have 30%～40% interior HSV of patient's body and HPV to detect positive approximately.Interferon can suppress the growth of antibacterial, also can suppress virus in host cell, duplicate and sphere of action extensive.
Description of drawings
Fig. 1 is polyurethane grafted figure under the field emission scanning electron microscope;
Fig. 2 is that laser scanning worker focusing microscope is for the light compositing interferon slice map on the polyurethane film;
Fig. 3 is fixed on bactericidal effect figure on the condom polyurethane for interferon-' alpha ';
Fig. 4 is the free bactericidal effect figure of interferon-' alpha ';
Wherein, among Fig. 1, A is no grafted polyurethane Electronic Speculum figure; B, C, D are the interferon grafted polyurethane Electronic Speculum figure of different proportion chi.Among Fig. 2, first passage is the FITC fluorescence channel, and second channel is a polyurethane surface transmission phase, and third channel is the stacks of two passages.
The specific embodiment
The grafting of embodiment 1 interferon
1. with IFN-α (30 μ g, contain inferior amide (the 61.5 μ g of N-succinum 0.002 μ mol) add 3ml, 0.2 in dimethylformamide DMF/PBS μ mol) (volume ratio is 4: the 1) solution, ice bath stirring reaction 48h (4 ℃), behind the end of synthesis, use the deutero-IFN-α of ultrafiltration membrane (milipore molecutII, 10k Na) purification azidophenyl respectively.
2. before the photo-immobilization polyurethane condom is cut into diameter and is positioned over 24 hole tissue culturing plates for the 10mm round sheet, do following the processing afterwards: respectively with photolytic activity IFN-α with six kinds of concentration (0.01 μ g, 0.025 μ g, 0.05 μ g, 0.075 μ g, 0.1 μ g, 0.25 μ g) add in the 24 hole tissue culturing plates, after having added medicine, Jiang Geban places refrigerator, lyophilization (4 ℃), (15W) shines 20min in the 2cm place down under uviol lamp then, utilize the very active characteristics of azido, active IFN be fixed on the PU material, behind the photo-immobilization with (pH7.4) solution cyclic washing PU30 time of no calcium magnesium phosphate buffer solution PBS (-).
The form of embodiment 2 electron microscopic observation photo-immobilization IFN-α
The A of Fig. 1, B, C, D is respectively the polyurethane surface behind the active IFN-α of observed polyurethane and grafting under the field emission scanning electron microscope, can see there is not the smooth empty of the synthetic polyurethane surface of photofixation, and form one deck orderly protein layer that is evenly distributed at polyurethane surface through the interferon of can seeing after fixing, also just like Fig. 4 D a spot of agglomeration appears then, because interferon molecule is little.From Fig. 1 D is example, observes with 20000 times, uses Imagepro plus5.0 that picture under the Electronic Speculum is analyzed, and parameter is as follows:
Table 1 is Fig. 1 D reunion data analysis
| Typical object area in： | pixels |
| Original count | 390 |
| Single objects | 351 |
| Clusters | 39 |
| Objects in clusters | 83 |
| Total objects | 434 |
| Typical object area | 13.05897 |
Table 2 is that the interferon particle shape of Fig. 1 D is learned data analysis
| Stats | Area | Diameter (mean) |
| Min | 5 | 2.874342 |
| (Obj.#) | 370 | 638 |
| Max | 43 | 10.98799 |
| (Obj.#) | 2588 | 10406 |
| Range | 38 | 8.113649 |
| Mean | 13.058974 | 4.323231 |
| Std.Dev | 8.6403408 | 1.28626 |
| Sum | 5093 | 1686.06 |
| Samples | 390 | 390 |
Computer has carried out scanning analysis to 10406 objects among Fig. 1 D, the granule that meets IFN has 390, the granule mean diameter is between 28.7nm and 109.9nm, mean diameter is 43.2nm, individual molecule granule number in visual field is approximately 351, particle agglomeration has 39 places, and the polyurethane surface of Figure 1A is very smooth, and the increase of surface atom number has caused the rapid variation of character.This surface atom number reduces with nano-particles size and the qualitative skin effect that causes after sharply increasing.We think that the polyurethane behind the photo-immobilization can think the nanometer level bioactive material, interferon particles is dispersed to the nanoscale structures size reduces, and the surface atom number is increased sharply, and specific surface area, surface area and Surface binding energy increase rapidly.From chemical terms, the increase of surface atom number, the deficiency of Atomic coordinate also have the residing bonding state of surface atom or bonding environment and original interferon molecule and polyurethane atom that very big difference is arranged, usually be in undersaturated condition, cause nano material to have high surface activity, be easy to combine with other atom.Possess free more not available other characteristics of pure interferon, progressively discharge interferon such as photolysis secondary amine key under certain condition.It is all relevant therewith that high catalytic activity that nano-particle shows and high response, nanoparticle are easy to reunite etc.Embodiment 3 laser scanning co-focusing microscopes (CSLM) are observed the form of IFN-α
Two passages carry out the burnt observation of scan laser microphotograph copolymerization, and first passage is observed the IFN-α of dyeing FITC, and second passage projection shows the polyurethane material surface, and the 3rd passage superposes.The material surface interferon is carried out six sections, obtain Fig. 2.
FITC cancellation extremely easily, but can the be special IFN-alpha protein be dyeed can see that the IFN molecule is not simply to be attached to material surface, but firm polyurethane surface and the biologically active of being grafted on.
Embodiment 4 bacteriostatic experiments
All experimental articles are made sterilization treatment in High Temperature High Pressure, respectively with three kinds of pathogenic bacterium (gonorrhea diplococcus, staphylococcus aureuses, Hemolytic streptococcus) in the culture medium flat plate purification, the individual plant bacterium colony appears, and choose the individual plant bacterium colony and insert physiological saline solution, microscopically is observed, and dilution is for about 10
5Bacteria suspension about CFU/ml (colony formine unit, colony-forming units), each is drawn 1ml and splashes into culture medium, and L type spreader is evenly coated each media surface.
Grafting concentration is divided into six, and the grafting face of photo-immobilization polyurethane is a single face, so use aseptic nipper to place the culture medium top layer material difference obverse and reverse of six concentration, uses L type spreader with bacterium liquid cladding material surface.
Blank controll is an aseptic culture medium, control2 is pure antibacterial culturing, and control3 is no grafting PU antibacterial culturing, and control4 is a latex thin film antibacterial culturing, control5 cultivates for free photolytic activity interferon maximum concentration, and control6 cultivates for the polyurethane powder maximum concentration.
Condition of culture: 37 ℃, 5% CO
2Incubator is cultivated gonorrhea diplococcus, and the common micro-organisms incubator is cultivated staphylococcus aureus and Hemolytic streptococcus for 37 ℃.
Behind 24h and the 72h, except control, other are cultivated and all find no obvious inhibition zone, concrete condition such as following table 3,4.
Table 3 is for cultivating the antibacterial situation of 24h
Table 4 is for cultivating the antibacterial situation of 72h
From table 3, the result of table 4 as can be seen, bigger among six contrasts owing to concentration, the efficacy of a drug of free light compositing interferon and pure interferon can be killed all antibacterials rapidly, very fast of diffusion velocity, and latex material is very fast by bacterial adhesion, there is not the PU of fixed drug in 24h, to have in the bacteriostasis property 72h gradually by bacterial adhesion, and tangible haemolysis circle appears in material below culture medium, even and the positive Cmin of placing of the PU of fixed medicine also can long term inhibition media surface bacterial growth, bacteriostasis rate reaches 100%, the haemolysis circle does not appear in the culture medium that the PU reverse side of fixed drug is placed under medicine, the bacteriostasis property of this proof interferon photo-immobilization polyurethane material is fine.
Embodiment 5 bactericidal effects relatively
Experimental technique: gonorrhea diplococcus is cultivated concentration about 10 as calculated
7CFU/ml, pour test tube A and B into, the A fixedly polyurethane material of the interferon of least concentration immerses test tube, B will identical grafting amount with the fixed drug polyurethane surface the least concentration interferon put into test tube, after 37 ℃ of shaking tables are cultivated one hour, detect A and B respectively with flow cytometer, the results are shown in Figure 3 and Fig. 4.
Experimental result: shown in Figure 4 as Fig. 3, the effect of antibacterial (gonorrhea diplococcus) effect specific ionization of fixing back interferon will be got well.After the interferon grafting was fixed on condom, the bactericidal activity in hour brought up to 6.3% by 2.1% of free interferon.
Claims (6)
1, a kind of antibiotic antiviral condom comprises antibacterial and antiviral drug and condom, it is characterized in that described antibacterial and antiviral drug is that grafting is fixed on the condom.
2, antibiotic antiviral condom according to claim 1, the material that it is characterized in that described condom is a polyurethane.
3, antibiotic antiviral condom according to claim 1 and 2 is characterized in that described antibacterial and antiviral drug is an interferon.
4, antibiotic antiviral condom according to claim 3 is characterized in that described interferon is LeIF or fiblaferon.
5, the preparation method of the described antibiotic antiviral condom of claim 1 is characterized in that adopting the photofixation method that the antibacterial and antiviral drug grafting is fixed in the contraception.
6, preparation method according to claim 5 is characterized in that comprising the steps: antibacterial and antiviral drug being added in the solution that contains the inferior amide of N-succinum the ice bath stirring reaction; Spread upon the condom outer surface then, under ultraviolet or 300～800nm visible light, shine.
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| Application Number | Priority Date | Filing Date | Title |
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| CNB2006100366600A CN100488471C (en) | 2006-07-25 | 2006-07-25 | Nano antibiotic antiviral condom |
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| CNB2006100366600A CN100488471C (en) | 2006-07-25 | 2006-07-25 | Nano antibiotic antiviral condom |
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| CN100488471C true CN100488471C (en) | 2009-05-20 |
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| CNB2006100366600A Expired - Fee Related CN100488471C (en) | 2006-07-25 | 2006-07-25 | Nano antibiotic antiviral condom |
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Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101569576B (en) * | 2008-05-04 | 2011-06-15 | 吴智尧 | Long-acting type condom with sterilization function and producing method thereof |
| CN102675464A (en) * | 2010-06-11 | 2012-09-19 | 华南师范大学 | Co-immobilized cytokine, preparation method and applications thereof |
| CN102532579A (en) * | 2012-01-11 | 2012-07-04 | 华南师范大学 | Antibacterial latex contraceptive material provided with grafted nanoscale IFN (Interferon)-alpha |
| CN103892956B (en) * | 2012-12-27 | 2018-10-19 | 浙江相伴乳胶制品有限公司 | A kind of sheath with antivirus action |
| CN103463671B (en) * | 2013-09-11 | 2015-04-22 | 华南师范大学 | Antibacterial and antiviral sanitary pad |
| CN107157640A (en) * | 2017-05-14 | 2017-09-15 | 郭宝煊 | Intelligent sheath |
| CN108379559A (en) * | 2018-05-23 | 2018-08-10 | 华中农业大学 | Application of the grass carp interferon 1 in preparing antibacterials |
| CN113208799A (en) * | 2021-06-07 | 2021-08-06 | 经鸿纬健康科技发展(上海)有限公司 | A polyurethane condom containing flavonoid antibacterial active factor |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2161043C1 (en) * | 2000-04-13 | 2000-12-27 | Гапонюк Петр Яковлевич | Agent of intimate designation for males |
| CN1408327A (en) * | 2001-09-03 | 2003-04-09 | 汪志新 | Nano condom |
| CN1154454C (en) * | 2000-12-20 | 2004-06-23 | 天津市亚马逊科技发展有限公司 | Nano composite condom and making method thereof |
| CN1686065A (en) * | 2005-06-09 | 2005-10-26 | 易乃君 | Safety enhanced condom |
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2006
- 2006-07-25 CN CNB2006100366600A patent/CN100488471C/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2161043C1 (en) * | 2000-04-13 | 2000-12-27 | Гапонюк Петр Яковлевич | Agent of intimate designation for males |
| CN1154454C (en) * | 2000-12-20 | 2004-06-23 | 天津市亚马逊科技发展有限公司 | Nano composite condom and making method thereof |
| CN1408327A (en) * | 2001-09-03 | 2003-04-09 | 汪志新 | Nano condom |
| CN1686065A (en) * | 2005-06-09 | 2005-10-26 | 易乃君 | Safety enhanced condom |
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