CN118103523A - 方法 - Google Patents

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Publication number
CN118103523A
CN118103523A CN202280036303.1A CN202280036303A CN118103523A CN 118103523 A CN118103523 A CN 118103523A CN 202280036303 A CN202280036303 A CN 202280036303A CN 118103523 A CN118103523 A CN 118103523A
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CN
China
Prior art keywords
leu
ala
gly
region
glu
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202280036303.1A
Other languages
English (en)
Chinese (zh)
Inventor
安德鲁·约翰·赫伦
理查德·亚历山大·古铁雷斯
约书亚·埃德尔
亚历山大·伊万诺夫
卡罗琳·科赫
薛梁
本尼迪克特·雷利-奥唐纳
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Imperial Institute Of Technology Innovation Co ltd
Oxford Nanopore Technology Public Co ltd
Original Assignee
Imperial Institute Of Technology Innovation Co ltd
Oxford Nanopore Technology Public Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Imperial Institute Of Technology Innovation Co ltd, Oxford Nanopore Technology Public Co ltd filed Critical Imperial Institute Of Technology Innovation Co ltd
Publication of CN118103523A publication Critical patent/CN118103523A/zh
Pending legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • G—PHYSICS
    • G11—INFORMATION STORAGE
    • G11C—STATIC STORES
    • G11C13/00—Digital stores characterised by the use of storage elements not covered by groups G11C11/00, G11C23/00, or G11C25/00
    • G11C13/0002—Digital stores characterised by the use of storage elements not covered by groups G11C11/00, G11C23/00, or G11C25/00 using resistive RAM [RRAM] elements
    • G11C13/0009—RRAM elements whose operation depends upon chemical change
    • G11C13/0014—RRAM elements whose operation depends upon chemical change comprising cells based on organic memory material
    • G11C13/0019—RRAM elements whose operation depends upon chemical change comprising cells based on organic memory material comprising bio-molecules

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CN202280036303.1A 2021-05-19 2022-05-19 方法 Pending CN118103523A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB2107192.3 2021-05-19
GBGB2107192.3A GB202107192D0 (en) 2021-05-19 2021-05-19 Method
PCT/GB2022/051268 WO2022243691A1 (en) 2021-05-19 2022-05-19 Multiplex methods of detecting molecules using nanopores

Publications (1)

Publication Number Publication Date
CN118103523A true CN118103523A (zh) 2024-05-28

Family

ID=76550727

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202280036303.1A Pending CN118103523A (zh) 2021-05-19 2022-05-19 方法

Country Status (8)

Country Link
US (1) US20250283153A1 (https=)
EP (1) EP4341427A1 (https=)
JP (1) JP2024522083A (https=)
CN (1) CN118103523A (https=)
AU (1) AU2022276473A1 (https=)
CA (1) CA3218861A1 (https=)
GB (1) GB202107192D0 (https=)
WO (1) WO2022243691A1 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB202301095D0 (en) 2023-01-25 2023-03-08 Oxford Nanopore Tech Plc Calibration of a nanopore array device

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US8828211B2 (en) 2010-06-08 2014-09-09 President And Fellows Of Harvard College Nanopore device with graphene supported artificial lipid membrane
KR101939420B1 (ko) 2011-02-11 2019-01-16 옥스포드 나노포어 테크놀로지즈 리미티드 돌연변이체 세공
EP4737389A2 (en) 2011-05-27 2026-05-06 Oxford Nanopore Technologies PLC Coupling method
CN104039979B (zh) 2011-10-21 2016-08-24 牛津纳米孔技术公司 使用孔和Hel308解旋酶表征目标多核苷酸的酶方法
GB201120910D0 (en) 2011-12-06 2012-01-18 Cambridge Entpr Ltd Nanopore functionality control
AU2012360244B2 (en) 2011-12-29 2018-08-23 Oxford Nanopore Technologies Limited Enzyme method
US9617591B2 (en) 2011-12-29 2017-04-11 Oxford Nanopore Technologies Ltd. Method for characterising a polynucleotide by using a XPD helicase
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KR102083695B1 (ko) 2012-04-10 2020-03-02 옥스포드 나노포어 테크놀로지즈 리미티드 돌연변이체 리세닌 기공
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SI3074534T1 (sl) * 2013-11-26 2019-08-30 Illumina, Inc. Postopki določanja zaporedja polinukleotidov
WO2015150786A1 (en) 2014-04-04 2015-10-08 Oxford Nanopore Technologies Limited Method for characterising a double stranded nucleic acid using a nano-pore and anchor molecules at both ends of said nucleic acid
GB201417712D0 (en) 2014-10-07 2014-11-19 Oxford Nanopore Tech Ltd Method
WO2016009180A1 (en) 2014-07-14 2016-01-21 Isis Innovation Limited Measurement of analytes with membrane channel molecules, and bilayer arrays
CA2959220A1 (en) 2014-09-01 2016-03-10 Vib Vzw Mutant csgg pores
US10480026B2 (en) * 2014-10-17 2019-11-19 Oxford Nanopore Technologies Ltd. Method for nanopore RNA characterisation
CN116200476A (zh) 2016-03-02 2023-06-02 牛津纳米孔科技公开有限公司 靶分析物测定方法、突变CsgG单体及其构筑体、及聚核苷酸和寡聚孔
GB201612458D0 (en) 2016-07-14 2016-08-31 Howorka Stefan And Pugh Genevieve Membrane spanning DNA nanopores for molecular transport
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GB201907244D0 (en) 2019-05-22 2019-07-03 Oxford Nanopore Tech Ltd Method

Also Published As

Publication number Publication date
US20250283153A1 (en) 2025-09-11
JP2024522083A (ja) 2024-06-11
AU2022276473A1 (en) 2023-11-30
CA3218861A1 (en) 2022-11-24
EP4341427A1 (en) 2024-03-27
GB202107192D0 (en) 2021-06-30
AU2022276473A9 (en) 2023-12-07
WO2022243691A1 (en) 2022-11-24

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