CN118056496A

CN118056496A - Ternary weeding composition containing flucloxapyroxad and application thereof

Info

Publication number: CN118056496A
Application number: CN202211445680.9A
Authority: CN
Inventors: 李平生; 姜若鲲; 赵德; 路兴涛
Original assignee: Jiangsu Qingyuan Nongguan Weed Control Co ltd
Current assignee: Jiangsu Qingyuan Nongguan Weed Control Co ltd
Priority date: 2022-11-18
Filing date: 2022-11-18
Publication date: 2024-05-21

Abstract

The invention belongs to the field of pesticides, and particularly relates to a ternary weeding composition containing flucloxapyroxad and application thereof. The ternary weeding composition comprises an active ingredient A, an active ingredient B and an active ingredient C in herbicidally effective amounts, wherein the active ingredient A is flucloxapyroxad; the active ingredient B is selected from one of the following compounds: topramezone, mesotrione, topramezone, isoxaflutole, fluroxypyr, tolpyralate; the active ingredient C is selected from one of the following compounds: atrazine, terbuthylazine. The composition can effectively prevent and kill off various weeds such as gramineae weeds, broadleaf weeds and the like, and has the characteristics of expanding a weed killing spectrum, reducing application amount, generating synergistic effect, solving resistant weeds and the like.

Description

Ternary weeding composition containing flucloxapyroxad and application thereof

Technical Field

The invention belongs to the field of pesticides, and particularly relates to a ternary weeding composition containing flucloxapyroxad and application thereof.

Background

Chemical weeding is the most economical and effective means in farmland weed control, but long-term continuous high-dosage chemical herbicide with single variety or single action mode is easy to cause problems of weed resistance, resistance evolution and the like.

The flucloxapyroxad is a biocidal herbicide, is widely applied to weeding of non-cultivated lands, no-tillage lands, farmland crops and the like, and plays an important role in preventing and killing weeds.

The reasonable compounding or compounding of the herbicide compound has the advantages of expanding the weed spectrum, improving the preventing and killing effect, delaying the occurrence and development of weed resistance and drug resistance, and the like, and is one of the most effective methods for solving the problems. Therefore, there is a need in the art to develop herbicidal compositions that are highly safe, broad-spectrum, synergistic and resistant to weeds.

Disclosure of Invention

In order to solve the problems in the prior art, the invention provides a ternary weeding composition containing flucloxapyroxad and application thereof, and the composition can effectively prevent and remove various weeds such as grasses, broadleaf weeds and the like, and has the characteristics of expanding a weeding spectrum, reducing application amount, generating synergistic effect, solving resistant weeds and the like.

A ternary herbicidal composition comprising fluramid, characterized in that it comprises a herbicidally effective amount of active ingredient A, active ingredient B and active ingredient C, wherein,

The active ingredient A is fluroxypyr ester (CAS number: 2445983-82-2);

The active ingredient B is selected from one of the following compounds: fenbuconazole (CAS number: 1992017-55-6), mesotrione (CAS number: 104206-82-8), topramezone (CAS number: 210631-68-8), isoxaflutole (CAS number: 141112-29-0), fluroxypyr (CAS number: 352010-68-5), tolpyralate (CAS number: 1101132-67-5);

The active ingredient C is selected from one of the following compounds: atrazine (CAS number: 1912-24-9), terbuthylazine (CAS number: 5915-41-3).

Wherein, the weight ratio of A, B to C is 1-200:1-150:1-1000, 20-180:5-120:50-700, 30-150:10-100:80-500, 45-120:20-80:120-350 or 60-100:30-50:180-250.

In a specific embodiment, the herbicidal composition is selected from any one of the following:

The active component A is fluchloramate, the active component B is fenpyraclonil, the active component C is atrazine, the weight ratio of A, B to C is 30-200:10-80:120-650, and the preferential weight ratio is 45-180:15-60:150-600;

The active component A is flucloxapyroxad, the active component B is fenbuconazole, the active component C is terbuthylazine, the weight ratio of A, B to C is 30-200:10-80:50-350, and the preferential weight ratio is 45-180:15-60:75-300;

The active component A is flucloxafen, the active component B is mesotrione, the active component C is atrazine, and the weight ratio of A, B to C is 30-200:20-150:120-650, preferably 45-180:30-120:150-600;

The active component A is flumetsulam, the active component B is mesotrione, the active component C is terbuthylazine, and the weight ratio of A, B to C is 30-200:20-150:50-350, preferably 45-180:30-120:75-300;

The active component A is flucloxapyroxad, the active component B is topramezone or Tolpyralate, the active component C is atrazine, the weight ratio of A, B to C is 30-200:5-40:120-650, and the preferential weight ratio is 45-180:7.5-30:150-600;

The active component A is flucloxapyroxad, the active component B is topramezone or Tolpyralate, the active component C is terbuthylazine, the weight ratio of A, B to C is 30-200:5-40:50-350, and the preferential weight ratio is 45-180:7.5-30:75-300;

The active ingredient A is clomazone, the active ingredient B is isoxaflutole, the active ingredient C is atrazine, and the weight ratio of A, B to C is 20-150:5-40:50-350, preferably 30-120:7.5-30:75-300;

The active component A is clomazone, the active component B is isoxaflutole, the active component C is terbuthylazine, the weight ratio of A, B to C is 20-150:5-40:50-250, and the preferential ratio is 30-120:7.5-30:60-240;

The active ingredient A is flucloxapyroxad, the active ingredient B is fluopicolide, the active ingredient C is atrazine, and the weight ratio of A, B to C is 20-150:5-30:50-350, preferably 30-120:6-24:75-300; or alternatively

The active component A is fluroxypyr ester, the active component B is fluopicolide, the active component C is terbuthylazine, and the weight ratio of A, B to C is 20-150:5-30:50-250, preferably 30-120:6-24:60-240.

The mass percentage of A, B and C in the weeding composition accounts for 1-95%, preferably 10-80% of the total mass.

The herbicidal composition further comprises conventional adjuvants including carriers and/or surfactants.

The term "carrier" as used herein means an organic or inorganic, natural or synthetic substance. They facilitate the application of the active ingredient, which carriers are generally inert and must be agriculturally acceptable, in particular by the treated plants. The carrier may be solid, such as clay, natural or synthetic silicate, silica, resin, wax, solid fertilizer, etc.; or liquid, such as water, alcohols, ketones, petroleum fractions, aromatic or waxy hydrocarbons, chlorinated hydrocarbons, liquefied gases, and the like.

Surfactants may include emulsifiers, dispersants or wetting agents, which may be ionic or nonionic. Examples which may be mentioned are salts of polyacrylic acid, lignosulfonates, salts of phenolsulfonic acid or naphthalenesulfonic acid, polymers of ethylene oxide with aliphatic alcohols or with aliphatic amines with substituted phenols, in particular alkylphenols or arylphenols, sulfosuccinates, taurine derivatives, in particular taurates, and phosphates of alcohols or of polyethylated phenols, alkylsulfonates, alkylaryl sulfonates, alkyl sulfates, lauryl ether sulfates, fatty alcohol sulfates, and also sulfated hexadecyl-, heptadeca-and octadecanols and sulfated fatty alcohol glycol ethers, furthermore condensates of naphthalene or naphthalenesulfonic acid with phenol and formaldehyde, polyoxyethylene octylphenyl ether, ethoxylated isooctylphenols, octylphenols or nonylphenols, alkylphenyl polyglycol ethers, tributylphenyl polyglycol ethers, tristearylphenyl polyglycol ethers, alkylaryl polyether alcohols, alcohol and fatty alcohol/ethylene oxide condensates, ethoxylated castor oil, polyoxyethylene alkyl ethers, ethoxylated polyoxypropylene, lauryl alcohol polyglycol ethers, sorbitol esters, lignin sulfite waste solutions, and also proteins, polysaccharides (e.g. methylcellulose), polyvinyl alcohol, hydrophobically modified polyvinyl pyrrolidone, polyvinyl alcohol carboxylate, polyvinyl pyrrolidone, and copolymers thereof. At least one surfactant is required to be present to facilitate the dispersion of the active ingredients in water and to enable them to be applied correctly to the plants.

The above-described compositions may also contain various other components such as protective colloids, binders, thickeners, thixotropic agents, penetrating agents, stabilizers, chelating agents, dyes, colorants and polymers.

The herbicidal composition further comprises at least one safener, preferably one or more of bisbenzoxazole acid (CAS: 163520-33-0), cyprosulfamide (CAS: 221667-31-8), pyraclonil (CAS: 135590-91-9), cloquintocet-mexyl (CAS: 99607-70-2), gibberellic acid (CAS: 7-06-5), furilazole (CAS: 121776-33-8), metcamifen (CAS: 129531-12-0).

In the context of the present specification, if the abbreviated form of the generic name of the active compound is used, all customary derivatives, such as esters and salts, as well as isomers, in particular optical isomers, in particular one or more commercially available forms, are included in each case. If the generic name denotes esters or salts, all other customary derivatives are also included in each case, such as other esters and salts, free acids and neutral compounds, as well as isomers, in particular optical isomers, in particular one or more commercially available forms. The chemical name given to a compound means at least one compound covered by a common name, generally the preferred compound. In the case of sulfonamides such as sulfonylureas, salts also include salts formed by the exchange of cations with hydrogen atoms in the sulfonamide group.

In the context of the present invention, the salts of the compounds are preferably in the form of the respective alkali metal, alkaline earth metal or ammonium salts, preferably in the form of the respective alkali metal salts, more preferably in the form of the respective sodium or potassium salts, most preferably in the form of the respective sodium salts.

The compositions of the present invention may be diluted by the user prior to use or used directly. The preparation can be prepared by common processing method, namely, mixing the active substance with liquid solvent or solid carrier, and adding one or more surfactants such as dispersing agent, stabilizer, wetting agent, adhesive, defoamer, etc.

The specific preparation of the weeding composition is dispersible oil suspending agent, water suspending agent, suspending emulsion, wettable powder, emulsifiable concentrate, water dispersible granule (dry suspending agent), water emulsion and microemulsion.

Briefly, the compositions of the present invention can be mixed with solid and liquid additives conventionally used in prior art formulations. The amount of the active ingredient used varies with the external conditions such as temperature, humidity, the nature of the herbicide used, and the like. It may have a large variation, for example between 0.001 and 1.0kg/ha, or more, of active substance, but preferably between 0.005 and 750g/ha, in particular between 0.005 and 500 g/ha.

In addition, the compositions of the invention can be applied by spraying onto the foliage of the plants to be treated, i.e. onto weeds, in particular onto surfaces which are infested or susceptible to infestation by weeds.

Further, the invention also provides application of the weeding composition in weed control; and a method of controlling unwanted plant growth comprising applying the herbicidal composition to plants, plant parts, plant seeds or areas of plant growth. Preferably, the herbicidal composition is used to selectively control weeds in crops of useful plants, more preferably in crops of useful plants which have been treated by transgenic or genome editing techniques.

The compounds of the invention are useful for treating all plants and plant parts. Plant varieties and cultivars can be obtained by conventional breeding and breeding methods or by genetic engineering methods. Genetically modified plants (transgenic plants) are those in which a heterologous gene (transgene) has been stably integrated into the genome of the plant. The transgene defined by its specific location in the plant genome is referred to as a transformation event or transgenic event.

Genetically modified plant cultivars that can be treated according to the invention include those that are resistant to one or more biotic stresses (pests such as nematode animals, insects, mites, fungi, etc.) or abiotic stresses (drought, low temperature, soil salinization, etc.), or which contain other desirable characteristics. Plants can be genetically modified to exhibit traits such as herbicide tolerance, insect tolerance, modified oil characteristics, or drought tolerance. Information on available genetically modified plants comprising a single gene transformation event or a combination of transformation events can be obtained from publicly available databases maintained, for example, by the U.S. department of agriculture.

When the herbicidal composition of the present invention is applied, its herbicidal activity achieves unexpected synergistic effects with respect to a single agent and any binary composition therein. The synergistic effect is manifested by a reduced application rate, a broader weed control spectrum, a faster and more durable herbicidal action, which are desirable in the practice of weed control. These new compositions are significantly superior to existing herbicides in terms of the characteristics described, achieving reduced use and being more environmentally friendly.

The synergistic weeding composition provided by the invention also has the following advantages:

(1) The composition of the invention is environment-friendly and is easy to degrade in the environment.

(2) The weeding composition disclosed by the invention is low in cost and convenient to use, and has great economic and social benefits in popularization and application.

Detailed Description

The following examples are not intended to limit the invention but are merely illustrative of how the invention may be practiced. For certain weeds, these examples showed particularly significant effectiveness. Examples are as follows:

a) Examples

1. Dispersible oil suspending agent of flucloxafen, fenpyraclonil and atrazine (6+3+22)

6% Of flucloxapyroxad, 3% of fenpyraclostrobin, 22% of atrazine, 4% of calcium dodecyl benzene sulfonate, 8% of cardanol polyoxyethylene ether, 8% of fatty acid polyoxyethylene ester, 1% of organic bentonite, 1% of fumed silica, 5% of 200# solvent oil and primary soybean oil are used for the balance

2. Dispersible oil suspending agent of flucloxafen, fenpyraclonil and terbuthylazine (6+3+10)

6% Of flucloxapyroxad, 3% of fenbuconazole, 10% of terbuthylazine, 3% of calcium dodecyl benzene sulfonate, 6% of cardanol polyoxyethylene ether, 6% of fatty acid polyoxyethylene ester, 2% of organic bentonite, 2% of fumed silica, 5% of 200# solvent oil and primary soybean oil are used for the balance

3. Flucarbazone, mesotrione and atrazine (6+5+20) dispersible oil suspending agent

6% Of flucloxapyroxad, 5% of mesotrione, 20% of atrazine, 4% of calcium dodecyl benzene sulfonate, 8% of cardanol polyoxyethylene ether, 8% of fatty acid polyoxyethylene ester, 1% of organic bentonite, 1% of fumed silica, 5% of 200# solvent oil and primary soybean oil are used for supplementing the balance

4. Flucarbazone, mesotrione and terbuthylazine (6+5+10) dispersible oil suspending agent

6% Of flucloxapyroxad, 5% of mesotrione, 10% of terbuthylazine, 3% of calcium dodecyl benzene sulfonate, 6% of cardanol polyoxyethylene ether, 6% of fatty acid polyoxyethylene ester, 1.5% of organic bentonite, 1.5% of fumed silica, 5% of 200# solvent oil and primary soybean oil are used for the balance

5. Dispersible oil suspending agent of flucloxafen, topramezone and atrazine (6+1+25)

6% Of flucloxapyroxad, 1% of topramezone, 25% of atrazine, 3% of calcium dodecyl benzene sulfonate, 8% of cardanol polyoxyethylene ether, 7% of fatty acid polyoxyethylene ester, 1% of organic bentonite, 1% of fumed silica, 5% of 200# solvent oil and primary soybean oil are used for supplementing the balance

6. Dispersible oil suspending agent of flucloxafen, topramezone and terbuthylazine (6+1+13)

6% Of flucloxapyroxad, 1% of topramezone, 13% of terbuthylazine, 3% of calcium dodecyl benzene sulfonate, 6% of cardanol polyoxyethylene ether, 6% of fatty acid polyoxyethylene ester, 1.5% of organic bentonite, 1.5% of gas-phase white carbon black, 5% of 200# solvent oil and primary soybean oil are used for the balance

7. Flucarbazone, isoxaflutole and atrazine (2+2+15) dispersible oil suspending agent

2% Of flucloxapyroxad, 2% of isoxaflutole, 15% of atrazine, 3% of calcium dodecyl benzene sulfonate, 6% of cardanol polyoxyethylene ether, 6% of fatty acid polyoxyethylene ester, 1% of organic bentonite, 1% of fumed silica, 3% of 200# solvent oil and primary soybean oil are used for supplementing the balance

8. Flucarbazone, isoxaflutole and terbuthylazine (4+2.5+25) suspoemulsion

4% Of flucloxapyroxad, 2.5% of isoxaflutole, 25% of terbuthylazine, 6% of 200# solvent oil, 2% of phenethyl phenol polyoxyethylene ether phosphate triethanolamine salt, 2.5% of castor oil polyoxyethylene ether, 2% of cardanol polyoxyethylene ether, 0.1% of xanthan gum, 2% of ethylene glycol, 0.05% of kathon and deionized water are used for the balance

9. Flucarbazone, fluroxypyr-meptyl and atrazine (5+1+12.5) dispersible oil suspending agent

5% Of flucloxapyroxad, 1% of flupirtine, 12.5% of atrazine, 3% of calcium dodecyl benzene sulfonate, 6% of cardanol polyoxyethylene ether, 6% of fatty acid polyoxyethylene ester, 2% of organic bentonite, 2% of fumed silica, 5% of 200# solvent oil and primary soybean oil are used for supplementing the balance

10. Flucarbazone, fluroxypyr-meptyl and terbuthylazine (5+1+10) dispersible oil suspending agent

5% Of flucloxapyroxad, 1% of fluopicolide, 10% of terbuthylazine, 3% of calcium dodecyl benzene sulfonate, 6% of cardanol polyoxyethylene ether, 6% of fatty acid polyoxyethylene ester, 2.5% of organic bentonite, 2% of fumed silica, 5% of 200# solvent oil and primary soybean oil are used for the balance

11. Flucarbazone+ Tolpyralate +atrazine (6+1+20) dispersible oil suspension

6% Of flucloxapyroxalate, 1% of Tolpyralate, 20% of atrazine, 5% of calcium dodecyl benzene sulfonate, 8% of cardanol polyoxyethylene ether, 5% of fatty acid polyoxyethylene ester, 1.2% of organic bentonite, 1% of gas-phase white carbon black, 5% of 200# solvent oil and primary soybean oil are used for the balance

12. Flucarbazone+ Tolpyralate +terbuthylazine (6+1+10) dispersible oil suspending agent

6% Of flucloxapyroxalate, 1% of Tolpyralate, 10% of terbuthylazine, 3% of calcium dodecyl benzene sulfonate, 6% of cardanol polyoxyethylene ether, 4% of fatty acid polyoxyethylene ester, 2% of organic bentonite, 2% of fumed silica, 5% of 200# solvent oil and primary soybean oil are used for the balance

The processing equipment of the suspension emulsion comprises: mixing kettle, colloid mill, sand mill, shearing machine, etc.

The processing process of the suspension emulsion comprises the following steps: adding the oil-soluble materials, the solvent and part of the emulsifier into a mixing kettle, and stirring until the oil-soluble materials, the solvent and part of the emulsifier are completely dissolved into an oil phase for later use; then, the oil-insoluble materials, part of emulsifying agent, thickening agent, antifreezing agent and the like are put into a mixing kettle, stirred and mixed, then the mixture is subjected to colloid mill, and finally the mixture is subjected to three-stage sand milling in a sand mill, so that a suspending agent is a water phase; pumping the water phase into a shearing kettle, slowly pumping the oil phase into the shearing kettle, shearing and pumping materials while shearing, and finally, sampling and testing after shearing is finished, and transferring to a storage tank for filling after the water phase is qualified.

The dispersible suspending agent processing equipment comprises: mixing kettle, colloid mill, sand mill, shearing machine, etc.

The processing process of the dispersible oil suspending agent comprises the following steps: all materials are put into a mixing kettle, stirred and mixed, then the materials are subjected to a colloid mill, and finally enter a sand mill for three-stage sand milling, finally the materials are uniformly sheared in a shearing machine, and after the materials are tested to be qualified, the materials are transferred to a storage tank for filling.

B) Test of efficacy

Post-emergence stem and leaf spray treatment:

1) Test conditions

1.1 Test target): bermudagrass root

Culturing weeds by a potting method, placing the weeds in a plastic nutrition pot with the diameter of 180 х mm in a enamel tray, arranging surface soil (4/5) which is collected from farmlands and air-dried and sieved, controlling the initial stage of soil humidity to be 20%, selecting weed seeds with full and uniform seeds, soaking the weed seeds in warm water at 25 ℃ for 6 hours, accelerating germination in a biochemical incubator (dark) at 28 ℃, uniformly placing the just-exposed weed seeds on the surface of the soil, and then covering the soil by 0.5-1cm according to the particle size of the seeds.

1.2 Culture conditions

The method is carried out in a controllable sunlight greenhouse, the temperature is 20-30 ℃, natural illumination is carried out, and the relative humidity is 57-72%. The soil type is loam, the organic matter content is 1.63%, the pH=7.1, the alkaline hydrolysis nitrogen content is 84.3mg/kg, the quick-acting phosphorus content is 38.5mg/kg, and the quick-acting potassium content is 82.1mg/kg.

1.3 Instrument and equipment

3WP-2000 type walking spray tower (Nanjing agricultural machinery institute of agricultural division); GA0 model ten-thousandth electronic balance (germany); ZDR2000 intelligent data logger (zengzhuze instruments limited); SPX intelligent biochemical incubator (Ningbo Jiangnan instruments factory).

2) Test design

2.1 Reagents)

The preparation method of the required active ingredient A is disclosed in patent CN201911321469.4; the desired active ingredient B, C is purchased from the reagent company or synthetically prepared according to conventional methods. The raw materials are all diluted by using acetone as solvent and 0.1% of emulsifier Tween-80 aqueous solution, and the diluted materials are diluted at present.

2.2 Test treatment

2.2.1 Dose setting)

In determining the ratio or content of A and active ingredient B, C, the main purpose of the formulation should be considered in consideration of the action characteristics of the medicament and its toxicity. The study was based on the preliminary pre-test, and the single use and the mixed use of A, B, C active ingredients are shown in the table. Water without agent, with the same solvent and emulsifier was used as a blank.

2.2.2 Test repetition

Each treatment was repeated 4 times, 3 pots each, 20 seeds of weed seeds were sown per pot, 60 plants per treatment.

2.3 Processing mode)

2.3.1 Time and number of treatments

The test was performed 1 time. And (3) after 1.5-2 leaves of weeds are in a period of thinning, 10 plants of weeds in each pot are maintained, 30 plants are maintained for each treatment, and then cultivation is continued until 3-4 leaves are subjected to treatment.

2.3.2 Device and method of administration

The cultured test materials are evenly placed on a platform with the area of 0.5m ², and sprayed by a 3WP-2000 type walking spray tower, wherein the spraying pressure is 0.3MPa according to the spraying amount of 450 kg/hectare. After all the liquid medicine is sprayed, the air valve is closed, the spraying tower door is opened after 30 seconds, and the nutrition pot is taken out. Then the air valve is opened, 50mL of clean water is sprayed, and the liquid spraying pipe is cleaned.

3) Test method

Potting method is adopted. Weed cultivation is 1.1), and is carried out by referring to herbicide as a rule of biological assay in pesticide room. The application method is 2.3.2), and stem and leaf treatment is adopted. After treatment, the cells are transferred into a greenhouse for routine cultivation.

4.1 Investigation method)

The entire seedlings of surviving weeds were cut off along the soil surface with a blade by an absolute number survey method, and the fresh weight of weeds was weighed with an analytical balance. For weeds that have died, the fresh weight is zero.

4.2 Time and number of surveys

The investigation was performed 21 days after the treatment, 1 total.

4.3 Data statistics analysis

And (3) evaluating the combined action type of any two and three mixed weeds by using a Colby method.

And at 21d, shearing green tissues on each treatment ground, weighing by adopting an electronic balance, and calculating the actual survival rate: e (%) = (fresh weed weight in treated area/fresh weed weight in control area) ×100

The theoretical weed survival rate calculation formula is: e0 (%) =x×y/100 (binary mixture)

Wherein X represents the survival rate of A at P, Y represents the survival rate of B at Q, E0 represents the theoretical survival rate of A+B at (P+Q), and E represents the actual survival rate of each treatment.

E0 (%) =x×y×z× … ×n/100 (N-1) (ternary and above mixed use)

Wherein X, Y, Z, N respectively represent the actual survival rate of n single agents, and n is the number of the compound herbicide varieties.

When E0-E >10%, it is shown that a synergistic effect is produced; antagonism is indicated when E0-E < -10%; when E0-E is between.+ -. 10%, this indicates that an additive effect is produced.

Soil sealing treatment:

1) Test conditions

1.1 Test target): bermudagrass root

The method is carried out in a controllable sunlight greenhouse, the temperature is 20-30 ℃, natural illumination is carried out, and the relative humidity is 55-75%.

The soil type is loam, the organic matter content is 1.63%, the pH=7.1, the alkaline hydrolysis nitrogen content is 84.3mg/kg, the quick-acting phosphorus content is 38.5mg/kg, and the quick-acting potassium content is 82.1mg/kg. The test soil was quantitatively loaded to 3/4 of the pot, and then irrigated from the bottom of the pot to completely wet the soil to a saturated state. The weed seeds to be tested are subjected to germination accelerating treatment until the weed seeds are exposed to white, then uniformly and quantitatively broadcast on the surface, and soil is covered by 0.5-1cm according to the size of the seeds, and the weed seeds are sowed for later use for 72 hours.

1.2 Instrument and equipment

GA10 model ten-thousandth electronic balance (germany); ZDR2000 intelligent data logger (zengzhuze instruments limited); SPX intelligent biochemical incubator (Ningbo Jiangnan Instrument factory), pipettor, etc.

2) Test design

2.1 Reagents)

2.1.1 Test agent

2.2 Test treatment

2.2.1 Dose setting)

In determining the ratio or content of component A and active ingredient B, C, the main purpose of the formulation should be considered in consideration of the characteristics of the drug and its toxicity. The study was based on the preliminary pre-test, and the single use and the mixed use of A, B, C active ingredients are shown in the table. Water without agent, with the same solvent and emulsifier was used as a blank.

2.2.2 Test repetition

Each treatment was repeated 4 times, 3 pots each time, each pot was sown with 30 weed seeds.

3) Test method

Irrigation was carried out before treatment, and a 1-2cm aqueous layer was maintained. Taking quantitative liquid medicine by a liquid shifter, and respectively irrigating from low dose to high dose according to test design, wherein each treatment is repeated for 4 times; after treatment, the cells were transferred to a greenhouse for routine cultivation, and a 1-2cm aqueous layer was maintained throughout the test period.

4) Data investigation and statistical analysis

The spraying treatment is the same as that of the stem and leaf after seedling, so the description is omitted.

The actual control of the individual components on weeds and the statistical results of the combined action evaluation are shown in tables 1 to 12 below. Except for the clear mark of 'soil sealing treatment', the other methods are 'post-seedling stem leaf spraying treatment'.

Table 1A shows that the clomazone, B shows that the fenbuconazole and C shows that the atrazine

Table 2A shows that the compound is flucloxapyroxad, B shows that the compound is fenbuconazole, and C shows that the compound is terbuthylazine

Table 3A shows the flucloxapyroxad, B the mesotrione, C the atrazine

Table 4A shows the flucloxapyroxad, B the mesotrione, C the terbuthylazine

Table 5A shows that the clomazone, B shows that the topramezone and C shows that the atrazine

Table 6A shows that the compound is flucloxapyroxad, B shows that the compound is topramezone, and C shows that the compound is terbuthylazine

Table 7A shows the flucloxapyroxad, B shows the isoxaflutole, and C shows the atrazine (soil sealing treatment)

Table 8A shows the flucloxapyroxad, B shows the isoxaflutole, and C shows the terbuthylazine (soil sealing treatment)

Table 9A shows the flucloxapyroxad, B shows the flupirtine, and C shows the atrazine (soil sealing treatment)

Table 10A shows the flucloxapyroxad, B shows the flupirtine, and C shows terbuthylazine (soil sealing treatment)

Table 11A shows that the clomazone, B is Tolpyralate, C is atrazine

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Table 12A shows that the fluramid, B is Tolpyralate, C is terbuthylazine

Note that: e (A): measured fresh weight survival of component A treatment at given dose

E (B): measured fresh weight survival of component B treatment at given dose

E (C): measured fresh weight survival of component C treatment at given dose

E (a+b): measured fresh weight survival of binary blends of component A and component B at a given dose

E (a+c): measured fresh weight survival of binary blends of component A and component C at a given dose

E (b+c): measured fresh weight survival of binary blends of component B and component C at a given dose

E (a+b+c): measured fresh weight survival rate for ternary blends of components A, B, C at a given dose

E ₀ (a+b+c): theoretical fresh weight survival rate of ternary blend of components A, B, C at given dose

E ₀ ((a+b) +c): theoretical fresh weight survival of component A, B as a whole binary blend with component C at a given dose

E ₀ ((a+c) +b): theoretical fresh weight survival of component A, C as a whole binary blend with component B at a given dose

E ₀ (a+ (b+c)): theoretical fresh weight survival of component B, C as a whole binary blend with component A at a given dose

C) Demonstration of field

The herbicide compositions prepared in examples 1 to 12 were used for field weed effect tests. Weeds occurring in the field are mainly: bermuda grass, hemostatic crabgrass, brown bristlegrass, dry barnyard grass, purslane, scandent hop and the like.

The test method comprises the following steps:

Stem and leaf treatment (F): when weeds are in the 3-4 leaf stage, a manual sprayer is used, the water adding amount is 30 kg/667 m ², and stem and leaf spraying is adopted for uniform spraying.

Soil treatment (S): before the germination of weeds, a manual sprayer is used, the water adding amount is 45 kg/667 m ², and soil surface spraying is adopted.

The specific test agents and dosages are shown in Table 13. The investigation of the control effect 28 days after the application is shown in Table 13.

Exemplary field effects of the formulated compositions described in Table 13

Examples	Dosage (g a.i./ha)	Treatment mode	Fresh weight control effect (%)
				1	930	F	97.2
2	570	F	99.5
				3	930	F	98.3
4	630	F	98.8
				5	960	F	95.7
6	600	F	96.1
				7	1140	S	98.4
8	945	S	95.6
				9	710.4	S	99.3
10	615	S	97.2
				11	810	F	95.8
12	510	F	96

Through a large number of experiments and researches, the composition is unexpectedly found to have a surprising and unexpected synergistic effect for preventing and killing various grassy weeds and broadleaf weeds, the synergistic effect is more remarkable in low dosage, the dosage can be reduced, the pollution to the environment is reduced, the agricultural cost is reduced due to reasonable compounding, the composition is efficient for ALS and ACCase inhibitor resistant weeds, and the composition has a good application prospect. Meanwhile, the test shows that the herbicide composition has good selectivity and excellent synergistic effect in corn fields, and can be developed into herbicide mixtures with wide market value.

Claims

1. A ternary herbicidal composition comprising fluramid, characterized in that it comprises a herbicidally effective amount of active ingredient A, active ingredient B and active ingredient C, wherein,

The active ingredient A is flumetofen;

The active ingredient B is selected from one of the following compounds: topramezone, mesotrione, topramezone, isoxaflutole, fluroxypyr, tolpyralate;

The active ingredient C is selected from one of the following compounds: atrazine, terbuthylazine.

2. The ternary herbicidal composition of claim 1 wherein the weight ratio of A, B to C is 1 to 200:1 to 150:1 to 1000, 20 to 180:5 to 120:50 to 700, 30 to 150:10 to 100:80 to 500, 45 to 120:20 to 80:120 to 350, or 60 to 100:30 to 50:180 to 250.

3. The ternary herbicidal composition of claim 1, wherein the active ingredient a is clomazone, the active ingredient B is fenpyraclonil, the active ingredient C is atrazine, the weight ratio of A, B to C is 30-200:10-80:120-650, preferably 45-180:15-60:150-600;

4. A ternary herbicidal composition according to any of claims 1-3, characterized in that the herbicidal composition comprises A, B and C in a mass percentage of 1-95%, preferably 10-80% of the total.

5. A ternary herbicidal composition according to any of claims 1-3, characterized in that it further comprises conventional adjuvants, preferably comprising carriers and/or surfactants.

6. A ternary herbicidal composition according to any of claims 1-3, characterized in that the herbicidal composition further comprises at least one safener, preferably selected from one or more of bisbenzoxazole acid, cyprosulfamide, mequindox, gibberellic acid, furilazole, metcamifen.

7. The ternary herbicidal composition of any one of claims 1-6, wherein the specific formulation of the herbicidal composition is a dispersible oil suspension, an aqueous suspension, a suspoemulsion, a wettable powder, an emulsifiable concentrate, a water dispersible granule, an aqueous emulsion or a microemulsion.

8. Use of a ternary herbicidal composition according to any of claims 1 to 7 for controlling weeds, preferably for selectively controlling weeds in crops of useful plants, more preferably in crops of useful plants which have been treated by transgenic or genomic editing techniques.

9. A method of controlling unwanted vegetation which comprises applying the ternary herbicidal composition of any one of claims 1 to 7 to plants, plant parts, plant seeds or areas of vegetation, preferably the herbicidal composition is used to selectively control weeds in crops of useful plants, more preferably the crops of useful plants are transgenic crops or crops of useful plants which have been treated by genome editing techniques.