CN1180248C - 优化电子杂交反应的方法 - Google Patents

优化电子杂交反应的方法 Download PDF

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Publication number
CN1180248C
CN1180248C CNB97197960XA CN97197960A CN1180248C CN 1180248 C CN1180248 C CN 1180248C CN B97197960X A CNB97197960X A CN B97197960XA CN 97197960 A CN97197960 A CN 97197960A CN 1180248 C CN1180248 C CN 1180248C
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CN
China
Prior art keywords
nucleic acid
damping fluid
hybridization
dna
target nucleic
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Expired - Fee Related
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CNB97197960XA
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English (en)
Chinese (zh)
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CN1230255A (zh
Inventor
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罗纳德·乔治·索斯诺思金
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威廉·福兰克·巴特勒
尤金·图
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麦克尔·爱尔文·耐仁伯格
��ղķ˹����Ƕ�
麦克尔·詹姆斯·海勒
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Nanogen Inc
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Nanogen Inc
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Publication of CN1230255A publication Critical patent/CN1230255A/zh
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • H—ELECTRICITY
    • H10—SEMICONDUCTOR DEVICES; ELECTRIC SOLID-STATE DEVICES NOT OTHERWISE PROVIDED FOR
    • H10D—INORGANIC ELECTRIC SEMICONDUCTOR DEVICES
    • H10D64/00—Electrodes of devices having potential barriers
    • H10D64/01—Manufacture or treatment
    • H10D64/021—Manufacture or treatment using multiple gate spacer layers, e.g. bilayered sidewall spacers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Pathology (AREA)
  • Electrochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
CNB97197960XA 1996-09-06 1997-08-18 优化电子杂交反应的方法 Expired - Fee Related CN1180248C (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US70826296A 1996-09-06 1996-09-06
US08/708,262 1996-09-06

Publications (2)

Publication Number Publication Date
CN1230255A CN1230255A (zh) 1999-09-29
CN1180248C true CN1180248C (zh) 2004-12-15

Family

ID=24845074

Family Applications (1)

Application Number Title Priority Date Filing Date
CNB97197960XA Expired - Fee Related CN1180248C (zh) 1996-09-06 1997-08-18 优化电子杂交反应的方法

Country Status (9)

Country Link
EP (1) EP1019711A4 (enExample)
JP (1) JP4213216B2 (enExample)
KR (1) KR100591626B1 (enExample)
CN (1) CN1180248C (enExample)
AU (1) AU723564B2 (enExample)
BR (1) BR9712800A (enExample)
CA (1) CA2264780C (enExample)
NZ (1) NZ334314A (enExample)
WO (1) WO1998010273A1 (enExample)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6051380A (en) * 1993-11-01 2000-04-18 Nanogen, Inc. Methods and procedures for molecular biological analysis and diagnostics
US7582421B2 (en) 1993-11-01 2009-09-01 Nanogen, Inc. Methods for determination of single nucleic acid polymorphisms using a bioelectronic microchip
US6468742B2 (en) 1993-11-01 2002-10-22 Nanogen, Inc. Methods for determination of single nucleic acid polymorphisms using bioelectronic microchip
US6379897B1 (en) * 2000-11-09 2002-04-30 Nanogen, Inc. Methods for gene expression monitoring on electronic microarrays
US5964995A (en) 1997-04-04 1999-10-12 Caliper Technologies Corp. Methods and systems for enhanced fluid transport
US6238909B1 (en) * 1999-05-04 2001-05-29 Motorola, Inc. Method and apparatus for obtaining electric field-enhanced bioconjugation
EP1216415A1 (en) * 1999-09-27 2002-06-26 Monsanto Technology LLC Methods for determining oils in seeds
US7309581B2 (en) * 2000-11-01 2007-12-18 Sysmex Corporation Method of staining, detection and counting bacteria, and a diluent for bacterial stain
GB0205455D0 (en) 2002-03-07 2002-04-24 Molecular Sensing Plc Nucleic acid probes, their synthesis and use
US7153687B2 (en) 2002-08-13 2006-12-26 Hong Kong Dna Chips Limited Apparatus and methods for detecting DNA in biological samples
JP3920223B2 (ja) * 2003-01-07 2007-05-30 日本碍子株式会社 反応性チップと、このチップを用いた標的物質の結合検出方法
JP4464664B2 (ja) * 2003-06-13 2010-05-19 独立行政法人理化学研究所 生体分子マイクロアレイ用基板、生体分子マイクロアレイ、相互作用促進用装置および方法、ならびに、相互作用の検出方法
US7314542B2 (en) * 2004-09-23 2008-01-01 Nanogen, Inc. Methods and materials for optimization of electronic transportation and hybridization reactions
KR100785011B1 (ko) * 2006-04-07 2007-12-11 삼성전자주식회사 쌍이온 화합물을 이용한 핵산 혼성화 특이성을 증가시키는방법
RU2315299C1 (ru) * 2006-09-20 2008-01-20 Государственное научное учреждение Всероссийский научно-исследовательский институт пищевой биотехнологии Российской академии сельскохозяйственных наук Рабочий электролит для определения капиллярным электрофорезом ионного состава жидких сред

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4936963A (en) * 1987-05-27 1990-06-26 Abbott Laboratories Polycationic buffers and method for gel electrophoresis of nucleic acids
US5188963A (en) * 1989-11-17 1993-02-23 Gene Tec Corporation Device for processing biological specimens for analysis of nucleic acids
US6017696A (en) * 1993-11-01 2000-01-25 Nanogen, Inc. Methods for electronic stringency control for molecular biological analysis and diagnostics
US5585069A (en) * 1994-11-10 1996-12-17 David Sarnoff Research Center, Inc. Partitioned microelectronic and fluidic device array for clinical diagnostics and chemical synthesis
KR100314996B1 (ko) * 1994-11-10 2002-01-15 윌리암 제이. 버크 액체분배시스템

Also Published As

Publication number Publication date
AU4071997A (en) 1998-03-26
WO1998010273A1 (en) 1998-03-12
KR100591626B1 (ko) 2006-06-20
EP1019711A4 (en) 2001-06-13
JP2001501301A (ja) 2001-01-30
JP4213216B2 (ja) 2009-01-21
KR20010029477A (ko) 2001-04-06
NZ334314A (en) 2000-09-29
AU723564B2 (en) 2000-08-31
CA2264780A1 (en) 1998-03-12
CN1230255A (zh) 1999-09-29
CA2264780C (en) 2006-08-01
EP1019711A1 (en) 2000-07-19
BR9712800A (pt) 1999-11-23

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