NZ334314A - Methods and materials for optimization of electronic hybridization reactions - Google Patents
Methods and materials for optimization of electronic hybridization reactionsInfo
- Publication number
- NZ334314A NZ334314A NZ334314A NZ33431497A NZ334314A NZ 334314 A NZ334314 A NZ 334314A NZ 334314 A NZ334314 A NZ 334314A NZ 33431497 A NZ33431497 A NZ 33431497A NZ 334314 A NZ334314 A NZ 334314A
- Authority
- NZ
- New Zealand
- Prior art keywords
- buffer
- hybridization
- microlocation
- electric field
- target nucleic
- Prior art date
Links
- 238000009396 hybridization Methods 0.000 title abstract 7
- 238000000034 method Methods 0.000 title abstract 5
- 238000006243 chemical reaction Methods 0.000 title abstract 3
- 238000005457 optimization Methods 0.000 title abstract 2
- 150000007523 nucleic acids Chemical class 0.000 abstract 6
- 102000039446 nucleic acids Human genes 0.000 abstract 6
- 108020004707 nucleic acids Proteins 0.000 abstract 6
- 239000000872 buffer Substances 0.000 abstract 5
- 230000005684 electric field Effects 0.000 abstract 4
- 235000018417 cysteine Nutrition 0.000 abstract 2
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 abstract 2
- 230000002708 enhancing effect Effects 0.000 abstract 2
- 238000004377 microelectronic Methods 0.000 abstract 2
- 235000001014 amino acid Nutrition 0.000 abstract 1
- 150000001413 amino acids Chemical class 0.000 abstract 1
- 239000012491 analyte Substances 0.000 abstract 1
- 239000007853 buffer solution Substances 0.000 abstract 1
- 230000003139 buffering effect Effects 0.000 abstract 1
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
-
- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01L—SEMICONDUCTOR DEVICES NOT COVERED BY CLASS H10
- H01L29/00—Semiconductor devices specially adapted for rectifying, amplifying, oscillating or switching and having potential barriers; Capacitors or resistors having potential barriers, e.g. a PN-junction depletion layer or carrier concentration layer; Details of semiconductor bodies or of electrodes thereof ; Multistep manufacturing processes therefor
- H01L29/66—Types of semiconductor device ; Multistep manufacturing processes therefor
- H01L29/66007—Multistep manufacturing processes
- H01L29/66075—Multistep manufacturing processes of devices having semiconductor bodies comprising group 14 or group 13/15 materials
- H01L29/66227—Multistep manufacturing processes of devices having semiconductor bodies comprising group 14 or group 13/15 materials the devices being controllable only by the electric current supplied or the electric potential applied, to an electrode which does not carry the current to be rectified, amplified or switched, e.g. three-terminal devices
- H01L29/66409—Unipolar field-effect transistors
- H01L29/66477—Unipolar field-effect transistors with an insulated gate, i.e. MISFET
- H01L29/6656—Unipolar field-effect transistors with an insulated gate, i.e. MISFET using multiple spacer layers, e.g. multiple sidewall spacers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6832—Enhancement of hybridisation reaction
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microelectronics & Electronic Packaging (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Power Engineering (AREA)
- General Physics & Mathematics (AREA)
- Microbiology (AREA)
- Manufacturing & Machinery (AREA)
- Ceramic Engineering (AREA)
- Condensed Matter Physics & Semiconductors (AREA)
- Pathology (AREA)
- Electrochemistry (AREA)
- Computer Hardware Design (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
Abstract
A method for optimization of electronic hybridzation reactions or a method for electronically enhancing hybridization of DNA analyte to single stranded capture DNA bound at a test site in an electric field at a positively biased test site on a microelectronic device has the following steps. Apply a buffer to the device and then apply a current to the test site on the microelectronic device in an amount sufficient to produce an electric field and provide a net positive charge on the buffer molecules. The buffer has certain conductance, natural isoelectric point and effective buffering capacity. In another method for enhancing the hybridization efficiency of target nucleic acids in a micorelectronic hybridization device including a microlocation test site having a capture nucleic acid, the microlocation is adapted to be placed at least in a first state wherein no electric field is generated and in a second state wherein an electric field attractive to the target nucleic acids is generated at the microlocation. The steps include applying a buffer to the device, provide target nucleic acid to the device, place the microlocation in the second state and hybridize the target nucleic acids with the capture nucleic acid. In particular, the methods describe how low conductance zwitterionic buffer solutions, especially those containing the amino acid Histidine prepared at concentrations of ~50 mM and at or near the pI (isoelectric point ~pH 7.47), provide optimal conditions for both rapid electrophoretic DNA transport and efficient hybridization reactions. Hybridization efficiencies of at least a factor of 10 relative to the next best known buffer, Cysteine, are achieved. Test data demonstrate an approximately 50,000 fold increase in hybridization efficiency compared to Cysteine.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US70826296A | 1996-09-06 | 1996-09-06 | |
PCT/US1997/014489 WO1998010273A1 (en) | 1996-09-06 | 1997-08-18 | Methods and materials for optimization of electronic hybridization reactions |
Publications (1)
Publication Number | Publication Date |
---|---|
NZ334314A true NZ334314A (en) | 2000-09-29 |
Family
ID=24845074
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
NZ334314A NZ334314A (en) | 1996-09-06 | 1997-08-18 | Methods and materials for optimization of electronic hybridization reactions |
Country Status (9)
Country | Link |
---|---|
EP (1) | EP1019711A4 (en) |
JP (1) | JP4213216B2 (en) |
KR (1) | KR100591626B1 (en) |
CN (1) | CN1180248C (en) |
AU (1) | AU723564B2 (en) |
BR (1) | BR9712800A (en) |
CA (1) | CA2264780C (en) |
NZ (1) | NZ334314A (en) |
WO (1) | WO1998010273A1 (en) |
Families Citing this family (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6051380A (en) * | 1993-11-01 | 2000-04-18 | Nanogen, Inc. | Methods and procedures for molecular biological analysis and diagnostics |
US6468742B2 (en) | 1993-11-01 | 2002-10-22 | Nanogen, Inc. | Methods for determination of single nucleic acid polymorphisms using bioelectronic microchip |
US6379897B1 (en) * | 2000-11-09 | 2002-04-30 | Nanogen, Inc. | Methods for gene expression monitoring on electronic microarrays |
US5964995A (en) * | 1997-04-04 | 1999-10-12 | Caliper Technologies Corp. | Methods and systems for enhanced fluid transport |
US6238909B1 (en) * | 1999-05-04 | 2001-05-29 | Motorola, Inc. | Method and apparatus for obtaining electric field-enhanced bioconjugation |
BR0014323A (en) * | 1999-09-27 | 2002-08-20 | Methods for determining the oil content of a seed from a batch seed sample, selecting a seed having an increased oil content, and introgressing a characteristic into a plant | |
US7309581B2 (en) * | 2000-11-01 | 2007-12-18 | Sysmex Corporation | Method of staining, detection and counting bacteria, and a diluent for bacterial stain |
GB0205455D0 (en) | 2002-03-07 | 2002-04-24 | Molecular Sensing Plc | Nucleic acid probes, their synthesis and use |
US7153687B2 (en) | 2002-08-13 | 2006-12-26 | Hong Kong Dna Chips Limited | Apparatus and methods for detecting DNA in biological samples |
JP4464664B2 (en) * | 2003-06-13 | 2010-05-19 | 独立行政法人理化学研究所 | Biomolecule microarray substrate, biomolecule microarray, device and method for promoting interaction, and method for detecting interaction |
US7314542B2 (en) * | 2004-09-23 | 2008-01-01 | Nanogen, Inc. | Methods and materials for optimization of electronic transportation and hybridization reactions |
KR100785011B1 (en) * | 2006-04-07 | 2007-12-11 | 삼성전자주식회사 | Method for increasing the specificity of nucleic acid hybridization using zwitterionic compounds |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4936963A (en) * | 1987-05-27 | 1990-06-26 | Abbott Laboratories | Polycationic buffers and method for gel electrophoresis of nucleic acids |
US5188963A (en) * | 1989-11-17 | 1993-02-23 | Gene Tec Corporation | Device for processing biological specimens for analysis of nucleic acids |
US6017696A (en) * | 1993-11-01 | 2000-01-25 | Nanogen, Inc. | Methods for electronic stringency control for molecular biological analysis and diagnostics |
AU705351B2 (en) * | 1994-11-10 | 1999-05-20 | Orchid Biosciences, Inc. | Liquid distribution system |
US5585069A (en) * | 1994-11-10 | 1996-12-17 | David Sarnoff Research Center, Inc. | Partitioned microelectronic and fluidic device array for clinical diagnostics and chemical synthesis |
-
1997
- 1997-08-18 JP JP51267698A patent/JP4213216B2/en not_active Expired - Lifetime
- 1997-08-18 CN CNB97197960XA patent/CN1180248C/en not_active Expired - Fee Related
- 1997-08-18 CA CA002264780A patent/CA2264780C/en not_active Expired - Fee Related
- 1997-08-18 WO PCT/US1997/014489 patent/WO1998010273A1/en active IP Right Grant
- 1997-08-18 NZ NZ334314A patent/NZ334314A/en unknown
- 1997-08-18 AU AU40719/97A patent/AU723564B2/en not_active Ceased
- 1997-08-18 BR BR9712800-7A patent/BR9712800A/en unknown
- 1997-08-18 EP EP97938378A patent/EP1019711A4/en not_active Withdrawn
- 1997-08-18 KR KR1019997001868A patent/KR100591626B1/en not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
JP4213216B2 (en) | 2009-01-21 |
WO1998010273A1 (en) | 1998-03-12 |
EP1019711A4 (en) | 2001-06-13 |
KR20010029477A (en) | 2001-04-06 |
JP2001501301A (en) | 2001-01-30 |
CA2264780C (en) | 2006-08-01 |
KR100591626B1 (en) | 2006-06-20 |
CA2264780A1 (en) | 1998-03-12 |
AU4071997A (en) | 1998-03-26 |
CN1230255A (en) | 1999-09-29 |
AU723564B2 (en) | 2000-08-31 |
EP1019711A1 (en) | 2000-07-19 |
BR9712800A (en) | 1999-11-23 |
CN1180248C (en) | 2004-12-15 |
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Legal Events
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