CN117970620B - Transmission illumination system and microscope - Google Patents
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- CN117970620B CN117970620B CN202410386070.9A CN202410386070A CN117970620B CN 117970620 B CN117970620 B CN 117970620B CN 202410386070 A CN202410386070 A CN 202410386070A CN 117970620 B CN117970620 B CN 117970620B
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- 238000005286 illumination Methods 0.000 title claims abstract description 86
- 230000005540 biological transmission Effects 0.000 title claims abstract description 58
- 230000003287 optical effect Effects 0.000 claims description 13
- 238000009792 diffusion process Methods 0.000 claims description 11
- 239000005338 frosted glass Substances 0.000 claims description 3
- 230000005499 meniscus Effects 0.000 claims description 3
- 210000001747 pupil Anatomy 0.000 claims description 3
- 230000002441 reversible effect Effects 0.000 claims description 3
- 230000000694 effects Effects 0.000 description 9
- 238000003384 imaging method Methods 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 239000002699 waste material Substances 0.000 description 3
- 230000009471 action Effects 0.000 description 2
- 239000000084 colloidal system Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000004075 alteration Effects 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 230000008094 contradictory effect Effects 0.000 description 1
- 238000013016 damping Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 230000001795 light effect Effects 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
Classifications
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/06—Means for illuminating specimens
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/06—Means for illuminating specimens
- G02B21/08—Condensers
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Abstract
The invention discloses a transmission illumination system and a microscope, wherein the microscope is provided with an objective lens or a plurality of objective lenses with different multiplying powers, the transmission illumination system comprises a light source, a light condensing module and a scatterer, the light condensing module is arranged at the downstream of a light path of the light source and used for condensing the emergent light of the light source, and the scatterer is arranged at the downstream of the light condensing module and used for diffusing the emergent light of the light condensing module; wherein different diffusers are selected downstream of the condensing module corresponding to the objective lenses with different magnifications; or the scatterer is a scatterer with a preset scattering range, and the objective lenses with different multiplying powers share the scatterer. The technical scheme of the invention aims to enable the output light of the transmission illumination system to fill the aperture diaphragm of each objective lens at low cost.
Description
Technical Field
The invention relates to the field of microscopes, in particular to a transmission illumination system and a microscope.
Background
The microscope is one of main technical means of microscopic observation, has extremely wide application field, and in order to expand application scenes, one microscope is usually matched with a plurality of objective lenses with different multiplying powers, so that the observation requirements of samples with different scales are met. However, the numerical apertures of different objective lenses are different, in general, the numerical aperture of a high power objective lens is far larger than that of a low power objective lens, and when the numerical aperture output by the transmission illumination system cannot meet the numerical aperture of the objective lens, the aperture stop of the objective lens cannot be completely filled, so that the observation effect is reduced. In general, the industry uses multiple sets of condensing lenses to match different objectives, for example, when switching from a low power objective to a high power objective, the condensing lenses in the transmission illumination system are also replaced simultaneously, so that the numerical aperture output by the transmission illumination system matches the numerical aperture of the objective as much as possible, but this approach leads to increased equipment cost.
Disclosure of Invention
The main object of the present invention is to propose a transmissive illumination system aimed at enabling the output light of the transmissive illumination system to fill the aperture stop of each objective at low cost.
To achieve the above object, the present invention provides a transmission illumination system applied to a microscope equipped with one objective lens or a plurality of objective lenses of different magnifications, comprising:
A light source;
The light condensing module is arranged at the downstream of the light path of the light source and is used for condensing the emergent light rays of the light source; and
The scattering body is arranged at the downstream of the light condensing module and used for diffusing emergent rays of the light condensing module;
the plurality of scattering bodies are arranged, the objective lenses are corresponding to different multiplying powers, and different scattering bodies are selected downstream of the light condensing module; or, the scattering angle of the scatterer is matched with the numerical aperture of the objective lens of the maximum magnification of the microscope.
Optionally, at least one of the diffusers is configured as a lambertian diffuser.
Optionally, a scattering angle of at least one of the scatterers is greater than or equal to 60 degrees.
Optionally, the plurality of scattering bodies are provided, and the plurality of scattering bodies are all rotatably arranged on the light emitting side of the light condensing module.
Optionally, the transmissive illumination system comprises a rotatable turntable, and the plurality of diffusers are spaced around the axis of rotation of the turntable.
Optionally, the turntable is arranged for damped rotation.
Optionally, the driving mode of the turntable is manual driving or electric driving.
Optionally, a plurality of the scatterers may be disposed in a reversible manner, so that each of the scatterers may be switched between a position opposite to the optical path of the light-condensing module and a position avoiding the optical path of the light-condensing module.
Optionally, the light source is configured as a planar LED and/or a white LED.
Optionally, the light source is configured as a hemispherical LED, and a diffusion plate is further disposed between the light source and the condensing module.
Alternatively, the diffusion plate is provided as a frosted glass plate or a translucent plate or a microlens array.
Optionally, the condensing module includes a first condensing lens and a second condensing lens.
Optionally, the first condensing lens is configured as a meniscus lens.
Optionally, the second condenser lens is configured as a biconvex lens.
Optionally, the light divergence angle of the light emitting side of the light condensing module is less than 10 degrees.
Optionally, the light source is arranged at an entrance pupil of the transmissive illumination system.
The invention also provides a microscope comprising the transmission illumination system.
In the technical scheme of the invention, after light rays emitted by the light source pass through the light condensing module, the divergence angle is smaller, a relatively uniform space illumination light spot is formed, the light rays irradiate on the scatterer, and the light rays are emitted towards the direction of the objective lens after being diffused by the scatterer. The divergence angle of the transmission illumination system is approximately equal to the root mean square of the sum of the divergence angles of the light condensing module and the scattering body, and the space illumination light spots irradiate different scattering bodies, so that different scattering effects can be obtained.
On the one hand, the scatterer is a scatterer with a preset scattering range, so that after the outgoing light of the light condensing module is diffused by the scatterer, the angle of the outgoing light of the transmission illumination system can be matched with the numerical aperture of the objective lens with the maximum multiplying power of the microscope, and therefore, the scatterer with the preset scattering range can be compatible with other objective lenses with low multiplying power, the objective lenses with different multiplying powers share one scatterer, and the angle of the outgoing light of the transmission illumination system can be matched with the numerical aperture of any objective lens installed by the microscope. Specifically, a scattering body with a preset scattering range can be configured for the transmission illumination system, the scattering angle of the scattering body is large enough, and after the scattering body diffuses light rays, the output light rays of the transmission illumination system can fill the aperture stops of all objective lenses. Compared with the prior art that a collecting lens is added or replaced, the cost of the scatterer designed by the application is relatively low, so that the output light of the transmission illumination system can be filled in the aperture diaphragm of each objective lens at low cost, and the observation effect of a microscope on a sample is ensured.
On the other hand, different diffusers are selected downstream of the condensing module corresponding to the objective lenses of different magnifications. Therefore, the transmission illumination system can be provided with a plurality of scattering bodies with different scattering angles, when the objective lenses with different multiplying powers are switched, the different scattering bodies are adaptively switched at the downstream of the light path of the light condensing module, and the divergence angle of the transmission illumination system can be changed, so that the output light ray angle of the transmission illumination system is matched with the numerical aperture of the objective lenses, and the light efficiency is fully utilized. In the adjustment process, only the switching of the scatterers is involved, so that the light focusing module does not need to be switched along with the switching, namely, only a plurality of scatterers with different scattering angles are required to be configured, the cost is low, and the operation is very convenient.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are required in the embodiments or the description of the prior art will be briefly described, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and other drawings may be obtained according to the structures shown in these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic diagram of a transmission illumination system according to an embodiment of the present invention;
FIG. 2 is a schematic view of an observation image of an embodiment of a transmission illumination system of the present invention;
FIG. 3 is a graph showing a light field intensity distribution of an embodiment of a light exit surface of a transmission illumination system according to the present invention;
FIG. 4 is a schematic diagram of a partial structure of a transmissive illumination system according to an embodiment of the present invention;
FIG. 5 is a schematic diagram of a system architecture of an embodiment of a microscope of the present invention.
Reference numerals illustrate:
The achievement of the objects, functional features and advantages of the present invention will be further described with reference to the accompanying drawings, in conjunction with the embodiments.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and fully with reference to the accompanying drawings, in which it is evident that the embodiments described are only some, but not all embodiments of the invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
It should be noted that, if directional indications (such as up, down, left, right, front, and rear … …) are included in the embodiments of the present invention, the directional indications are merely used to explain the relative positional relationship, movement conditions, etc. between the components in a specific posture (as shown in the drawings), and if the specific posture is changed, the directional indications are correspondingly changed.
In the present invention, unless specifically stated and limited otherwise, the terms "connected," "affixed," and the like are to be construed broadly, and for example, "affixed" may be a fixed connection, a removable connection, or an integral body; can be mechanically or electrically connected; either directly or indirectly, through intermediaries, or both, may be in communication with each other or in interaction with each other, unless expressly defined otherwise. The specific meaning of the above terms in the present invention can be understood by those of ordinary skill in the art according to the specific circumstances.
In addition, if there is a description of "first", "second", etc. in the embodiments of the present invention, the description of "first", "second", etc. is for descriptive purposes only and is not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include at least one such feature. In addition, if the meaning of "and/or" is presented throughout this document, it is intended to include three schemes in parallel, taking "a and/or B" as an example, including a scheme, or B scheme, or a scheme where a and B meet simultaneously. In addition, the technical solutions of the embodiments may be combined with each other, but it is necessary to base that the technical solutions can be realized by those skilled in the art, and when the technical solutions are contradictory or cannot be realized, the combination of the technical solutions should be considered to be absent and not within the scope of protection claimed in the present invention.
The invention provides a transmission illumination system. Without loss of generality, the transmission illumination system of the invention is a bright field transmission illumination system, the light source 100 irradiates a sample with light transmission property from bottom to top, illumination light directly enters an objective lens after passing through the sample, and imaging is captured by a camera.
In one embodiment of the present invention, as shown in fig. 1 and 5, the transmission illumination system includes:
A light source 100;
The light condensing module 200 is arranged at the downstream of the light path of the light source 100 and is used for condensing the emergent light rays of the light source 100; and
A diffuser 300, disposed downstream of the light-condensing module 200, for diffusing the outgoing light of the light-condensing module 200;
the microscope is provided with an objective lens 600 or a plurality of objective lenses 600 with different magnifications;
Wherein different diffusers 300 are selected downstream of the condensing module 200 corresponding to the objective lenses 600 of different magnifications; or, the scatterer 300 is a scatterer with a preset scattering range, and the objective lens 600 with different magnifications shares the scatterer 300.
In the technical scheme of the invention, after passing through the condensing module 200, the light rays emitted by the light source 100 have smaller divergence angles, form a relatively uniform space illumination light spot, irradiate on the scatterer 300, and are emitted towards the direction of the objective lens 600 after being diffused by the scatterer 300. The outgoing light from the scatterer 300 passes through the sample 500 placed on the stage 400, forms transmitted light, and enters the objective lens 600, and is finally captured by the photographing system 700. The viewing magnification of the system may be adapted by changing the objective lens 600 for different viewing magnification requirements.
The surface of the diffuser 300 is provided with particles with different dimensions, or the material itself is a diffuser colloid material, and the light incident on the surface is deflected by using random diffusion of the particles with different dimensions, or by using the diffusion effect of the colloid material itself, so as to form light diffusion. The divergence angle of the transmission illumination system is approximately the root mean square of the sum of the divergence angle of the condensing module 200 and the divergence angle of the diffuser 300, and the space illumination light spots irradiate different diffusers 300, so that different scattering effects can be obtained.
In one aspect, the scatterer 300 is a scatterer with a preset scattering range, so that after the outgoing light of the light condensing module is diffused by the scatterer 300, the angle of the outgoing light of the transmission illumination system can be matched with the numerical aperture of the objective lens 600 with the maximum magnification of the microscope, and thus, the scatterer with the preset scattering range can be compatible with other objective lenses 600 with low magnification, and the objective lenses 600 with different magnifications share one scatterer 300, so that the angle of the outgoing light of the transmission illumination system can be matched with the numerical aperture of any objective lens installed by the microscope. Specifically, a scattering body 300 with a preset scattering range may be configured for the transmission illumination system, where the scattering angle of the scattering body 300 is large enough, and after the scattering body 300 diffuses the light, the output light of the transmission illumination system can fill the aperture stops of all the objective lenses. When the microscope is provided with only one objective lens, under the action of the scatterer, the emergent light of the transmission illumination system can fill the aperture diaphragm of the objective lens; when the microscope is provided with a plurality of objective lenses with different magnifications, the scatterer can be compatible with other objective lenses with low magnifications of the microscope. Compared with the prior art of adding or replacing a condenser, the cost of the scatterer 300 designed by the application is relatively low, so that the output light of the transmission illumination system can be filled in the aperture diaphragm of each objective lens at low cost, and the observation effect of a microscope on a sample is ensured. As shown in fig. 2, in the case where the scattering body 300 having the divergence angle matching the numerical aperture of the objective lens having the maximum magnification is arranged in the transmission illumination system, the objective lens is switched only, and the obtained observation image is not subjected to other operations (that is, the members of the transmission illumination system are not replaced), and it is understood that the observation effect can be improved even for the objective lens having different magnification on the premise that the divergence angle of the scattering body 300 matches the numerical aperture of the objective lens having the maximum magnification.
Without loss of generality, the diffuser of the transmissive illumination system configuration is a lambertian diffuser. It will be appreciated that the relationship between the angle and intensity of the emitted light rays of the lambertian diffuser 300 satisfies the lambertian distribution, the angular spectrum of which is shown in fig. 3. The lambertian distribution is also called cosine distribution, and refers to light rays emitted from different angles, and the intensity of the light rays is approximately equal to the cosine value of the emission angle. For the diffuser 300 with the lambertian scattering mode, when the diffuser emits light forwards, the light intensity is maximum, the emergent light energy of the light-gathering module 200 keeps good spatial uniformity on the surface of the diffuser 300, the divergence angle is greatly increased, and the aperture stops of all objective lenses with numerical aperture values below 0.9 can be filled to meet the observation requirement. Of course, in other embodiments, the diffuser may be configured as a common light homogenizing sheet, and the scattering angle is greater than or equal to 60 degrees, that is, the angle of the output light of the transmission illumination system can be matched with the numerical aperture of any objective lens installed on the microscope.
On the other hand, the different diffusers are selected downstream of the condensing module corresponding to the objective lenses of different magnifications. In this way, the transmission illumination system may be configured with a plurality of diffusers with different scattering angles, and when the objective lens with different multiplying power is switched, the different diffusers 300 are adaptively switched downstream of the optical path of the light condensing module 200, so that the divergence angle of the transmission illumination system may be changed, and the output light angle of the transmission illumination system is matched with the numerical aperture of the objective lens, so as to fully utilize the light efficiency. For example, for an objective lens with NA0.5, switching a diffuser 300 with a diffuser angle of 25 ° or approximately 25 °, the output numerical aperture of the transmissive illumination system may be adjusted to match the objective lens, thereby allowing the objective lens to more efficiently receive light from the transmissive illumination system.
It will be appreciated that for high angle illumination beyond the numerical aperture of the objective lens, it is virtually impossible to effectively participate in imaging, and therefore in some application scenarios, such as insufficient light power of the light source, or thicker observed sample slices, or lower sample transmission coefficients, the intensity of the illumination light after the illumination light path passes through the sample may be lower, and at this time, the numerical aperture output by the transmission illumination system is too large, which may result in waste of light energy.
Therefore, to match the observation requirements of objective lenses with different magnifications, it is necessary to adaptively adjust the divergence angle of light rays output by the transmission illumination system according to the numerical aperture of the objective lens.
In the invention, different objective lenses can be matched by switching different scatterers so as to fully utilize the light effect. Because the adjustment process only involves the switching of the scatterers 300, the light focusing module 200 does not need to be switched accordingly, that is, only a plurality of scatterers 300 with different scattering angles need to be configured, so that the cost is low and the operation is very convenient.
Specifically, the plurality of diffusers 300 are switchably disposed on the light-emitting side of the light-condensing module 200, different diffusers 300 are disposed at different scattering angles, and at least one of the diffusers 300 is disposed downstream of the light-condensing module. In this way, different diffusers 300 can be adaptively adjusted downstream of the optical path of the condensing module 200 corresponding to different objective lenses. One diffuser 300 may be selected to be disposed downstream of the optical path of the condensing module 200 in correspondence with an objective lens, or two or more diffusers 300 may be selected to be disposed downstream of the optical path of the condensing module 200 in a stacked manner. Further, among the plurality of diffusers 300, at least one diffuser 300 is configured as a lambertian diffuser to satisfy an aperture stop of an objective lens of a maximum magnification.
Further, in the present embodiment, the plurality of scattering bodies 300 are rotatably disposed on the light emitting side of the light condensing module 200. Thus, the switching of different diffusers 300 can be realized by rotating the diffusers 300, and the operation is very convenient. Of course, in other embodiments, the diffuser 300 may be provided on the light emitting side of the light collecting module 200 so as to be retractable, and when the diffuser 300 is switched, the old diffuser 300 may be extracted and a new diffuser 300 may be inserted.
Further, in the present embodiment, as shown in fig. 4, the transmission illumination system includes a rotatable turntable 400, and a plurality of the scattering bodies 300 are spaced apart along the circumference of the turntable 400. It will be appreciated that the central axis of the turntable 400, i.e. the axis of rotation thereof, a plurality of scatterers 300 are distributed around the axis of rotation of the turntable 400. Thus, by rotating the turntable 400, the matched diffuser 300 can be driven to move to the downstream of the light path of the condensing module 200 and opposite to the objective lens. The turntable 400 can be arranged on the microscope in a damping and rotating manner, and the turntable 400 can be maintained at an original position under the action of no external force, so that an operator can observe a sample conveniently. When an operator needs to switch the scatterer 300, a certain acting force is applied to drive the turntable 400 to rotate; alternatively, the turntable 400 may be driven by a motor, and an operator inputs a corresponding instruction through a button or a display panel to drive the motor to drive the matched diffuser 300 to the light path downstream of the condensing module 200. Of course, in other embodiments, a plurality of diffusers 300 may be disposed in a reversible manner, so that each diffuser 300 can be switched between a position corresponding to the optical path of the light-collecting module 200 and a position corresponding to the optical path of the light-collecting module 200, and when switching the diffusers 300, the unused diffusers 300 may be turned upside down, the outgoing optical path of the light-collecting module 200 may be avoided, and the desired diffuser 300 may be disposed corresponding to the outgoing optical path of the light-collecting module 200.
In one embodiment, the light source 100 is configured as a planar LED (LIGHT EMITTING Diode). In this manner, the light source 100 may provide a higher illumination field uniformity and facilitate a compact design of the transmissive illumination system to reduce the footprint of the transmissive illumination system. Of course, in other embodiments, the light source 100 may be an LCD or Micro LED, etc.
Further, in the present embodiment, the light source 100 is configured as a white LED. Thus, the service life of the light source 100 can be ensured, and the light source 100 generates less heat, thereby being beneficial to ensuring the stability of the transmission illumination system. Of course, in other embodiments, the light source 100 may be configured as a light source 100 of other colors.
In an embodiment, the light source 100 is arranged at the entrance pupil of the transmission illumination system. Thus, the light source image can be eliminated, and the light beams emitted by each point of the light source 100 can be respectively filled into different coordinate positions of the aperture diaphragm of the transmission illumination system, so that the imaging effect of the microscope is ensured.
In an embodiment, the light source 100 is configured as a hemispherical LED, and a diffusion plate is further disposed between the light source 100 and the condensing module 200. In this embodiment, the spherical aberration of the hemispherical LED is eliminated by the planar diffusion plate, so that the diffusion plate becomes the second sub-light source, and the outgoing light of the light source 100 is adjusted to be planar, which can also provide higher uniformity of the illumination light field, and is beneficial to compact design of the transmission illumination system, so as to reduce the occupation space of the transmission illumination system. Specifically, the diffusion plate is provided as a frosted glass plate. Of course, in other embodiments, the diffusion plate may be a translucent plate or a microlens array, etc.
In one embodiment, the condensing module 200 includes a first condensing lens 210 and a second condensing lens 220. In this way, the outgoing light of the light source 100 can be converged by at least two condensing lenses, so that the light divergence angle of the light outgoing side of the condensing module 200 is ensured to be small enough to be fully accepted by the diffuser 300, so as to avoid the waste of light efficiency. Of course, in other embodiments, the condensing module 200 may also include a diverging lens, which only needs to ensure that the condensing capability of the condensing module 200 on the light can meet the requirement.
Specifically, the first condensing lens 210 is configured as a meniscus lens, and can collect light rays with a large angle to ensure effective utilization of the light emitted from the light source 100, and the first condensing lens 210 can provide a larger positive focal power to ensure the converging effect on the light rays emitted from the light source 100; the second condenser lens 220 is configured as a lenticular lens, and collimates the high-angle light rays of the fringe field of view so that the principal rays thereof are as parallel as possible to the optical axis. Therefore, the light efficiency can be fully utilized, and the imaging quality of a microscope can be ensured. Of course, in other embodiments, the first condensing lens 210 and the second condensing lens 220 may be configured as other planar lenses, such as plano-convex lenses.
In an embodiment, the divergence angle of the light rays on the light emitting side of the light condensing module 200 is less than 10 degrees. In this way, the outgoing light of the light condensing module 200 can be ensured to be projected onto the diffuser 300, so as to avoid the waste of light efficiency.
The invention also provides a microscope, which comprises a transmission illumination system, and the specific structure of the transmission illumination system refers to the embodiment, and because the microscope adopts all the technical schemes of all the embodiments, the microscope at least has all the beneficial effects brought by the technical schemes of the embodiments, and the detailed description is omitted.
The foregoing description is only of the optional embodiments of the present invention, and is not intended to limit the scope of the present invention, and all the equivalent structural changes made by the description of the present invention and the accompanying drawings or direct/indirect application in other related technical fields are included in the scope of the present invention.
Claims (11)
1. A transmission illumination system for use with a microscope having one or more objectives of different magnifications, the transmission illumination system being a bright field transmission illumination system, the transmission illumination system comprising:
A light source;
The light condensing module is arranged at the downstream of the light path of the light source and is used for condensing the emergent light rays of the light source; and
The scattering body is arranged at the downstream of the light condensing module and used for diffusing emergent rays of the light condensing module; the emergent ray of the scattering body is projected on the sample on the objective table;
Wherein a plurality of scattering bodies are arranged, the scattering angles of the plurality of scattering bodies are different, the plurality of scattering bodies are rotatably arranged on the light emitting side of the light condensing module, the downstream of the condensing module selects the scatterers with different scattering angles corresponding to the objective lenses with different multiplying powers.
2. The transmissive illumination system of claim 1, wherein at least one of the diffusers is configured as a lambertian diffuser; and/or, the scattering angle of at least one scattering body is greater than or equal to 60 degrees.
3. The transmissive illumination system of claim 1, wherein the transmissive illumination system comprises a rotatable turntable, a plurality of the diffusers being spaced around an axis of rotation of the turntable.
4. A transmissive illumination system as recited in claim 3, wherein the turntable is arranged for damped rotation; and/or the driving mode of the turntable is manual driving or electric driving.
5. The transmissive illumination system of claim 1, wherein a plurality of the diffusers are arranged in a reversible layer such that each diffuser is switchable between a position relative to the optical path of the light gathering module and a position to clear the optical path of the light gathering module.
6. The transmissive illumination system of claim 1, wherein the light source is configured as a planar LED and/or a white LED; or, the light source is configured as a hemispherical LED, and a diffusion plate is further arranged between the light source and the light condensing module.
7. The transmissive illumination system of claim 6, wherein the diffuser plate is provided as a frosted glass plate or a translucent plate or a microlens array.
8. The transmissive illumination system of claim 1, wherein the condensing module comprises a first condensing lens and a second condensing lens.
9. The transmissive illumination system of claim 8, wherein the first condenser lens is configured as a meniscus lens;
And/or the second condenser lens is configured as a biconvex lens.
10. The transmissive illumination system according to any one of claims 1 to 9, wherein a ray divergence angle of the light exiting side of the condenser module is less than 10 degrees;
and/or the light source is arranged at an entrance pupil of the transmission illumination system.
11. A microscope comprising a transmission illumination system according to any one of claims 1 to 10.
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|---|---|---|---|---|
| US5880861A (en) * | 1996-04-08 | 1999-03-09 | Olympus Optical Co., Ltd. | Illumination optical system |
| CN104459969A (en) * | 2014-12-08 | 2015-03-25 | 天津大学 | Biological microscope illuminating system based on engineering diffuser |
| CN111338069A (en) * | 2020-04-13 | 2020-06-26 | 合肥登特菲医疗设备有限公司 | Oral cavity microscope lighting device |
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