CN104459969A - Biological microscope illuminating system based on engineering diffuser - Google Patents
Biological microscope illuminating system based on engineering diffuser Download PDFInfo
- Publication number
- CN104459969A CN104459969A CN201410746244.4A CN201410746244A CN104459969A CN 104459969 A CN104459969 A CN 104459969A CN 201410746244 A CN201410746244 A CN 201410746244A CN 104459969 A CN104459969 A CN 104459969A
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- China
- Prior art keywords
- engineering diffuser
- diffuser
- engineering
- biological microscope
- illuminating
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/06—Means for illuminating specimens
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/06—Means for illuminating specimens
- G02B21/08—Condensers
- G02B21/082—Condensers for incident illumination only
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- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Optics & Photonics (AREA)
- Microscoopes, Condenser (AREA)
Abstract
The invention relates to the technical field of microscopes and provides a biological microscope illuminating system based on an engineering diffuser. The uniformity of lighting illuminance of an object table is improved, and the service life of the illuminating system is prolonged. Meanwhile, the structure of the illuminating system is simplified. According to the technical scheme, the biological microscope illuminating system based on the engineering diffuser comprises a light-emitting diode LED (1), a converging lens (2), the engineering diffuser (3) and the object table (4). The LED (1) provides high-intensity illuminating light beams. The converging lens (2) receives and converges the illuminating light beams. The engineering diffuser (3) controls the illuminating light beams to control the spatial distribution and the intensity outline of the illuminating light beams so that the illuminance of the illuminating light beams can be uniformly projected on biological samples which are placed on the object table (4) and are to be detected. The illuminating system is mainly applied to design and manufacture of microscope illuminating devices.
Description
Technical field
The present invention relates to microscopy field; Specifically, the biological microscope illuminator based on engineering diffuser is related to.
Technical background
In biological microscope technical field, the design for illuminator has higher requirement, the requirement of the not only requirement of the larger illumination zone of demand fulfillment, higher illumination intensity, and demand fulfillment illumination light uniform-illumination.Specifically, for the biological microscope with low power objective, illumination intensity less demanding, but, need larger illumination zone and higher illumination light illuminance uniformity; For the biological microscope with high power objective, need higher illumination intensity and illumination light illuminance uniformity, illumination zone less demanding.At present, the illuminator of biological microscope mainly adopts transmitted light bright field to throw light on, and is mainly divided into critical illumination and Kohler illumination two kinds of modes.Wherein, the picture of light source becomes on sample surfaces (object plane) by critical illumination, but the unevenness of light source surface brightness can affect microscopical observing effect; Kohler illumination eliminates the even shortcoming of sample surfaces in critical illumination (object plane) glazing uneven illumination by the setting of Ke Lejing, but, there is the light channel structure of more complicated.Thus, need to introduce new technology---engineering diffuser, on the maintenance simple basis of lighting system structure, improve the homogeneity of sample surfaces illuminance.Simultaneously, the illuminator of traditional biological microscope generally uses Halogen lamp LED or tungsten lamp as light source, the requirement of illumination intensity can be met, but, serviceable life is shorter, thus, the high strength of LED and the advantage of long service life needs in the design of the illuminator being introduced into biological microscope, increases the serviceable life of illuminator.
Summary of the invention
For overcoming the deficiencies in the prior art, providing a kind of biological microscope illuminator based on engineering diffuser, improving the homogeneity of objective table place illumination light illumination; Increase the serviceable life of illuminator.Meanwhile, the simplification of lighting system structure is realized further.For this reason, the technical scheme that the present invention takes is, based on the biological microscope illuminator of engineering diffuser, comprising: LED (1), convergent lens (2), engineering diffuser (3), objective table (4); LED (1) provides the illuminating bundle of high strength; Convergent lens (2) receives and assembles illuminating bundle; Engineering diffuser (3) controls illuminating bundle, controls space distribution and the intensity profile of illuminating bundle, makes the illuminance of illuminating bundle project the upper biological sample to be detected placed of objective table (4) equably.
Engineering diffuser (3) is made up of lenticule cell array, and each lenticule unit forms diffuser, and being determined by its sag profile and position in an array, the distribution of lenticule unit is random.
The distribution of lenticule unit is determined according to the probability distribution function produced selected by corresponding beam shape function.
The making of engineering diffuser (3) is realized by laser ablation, by raster scan pattern pointwise exposed photoresist layer, meanwhile, the intensity of adjustment laser beam changes photoresist depth of exposure, forms the multi-form lenticule unit being positioned at diverse location.
Compared with the prior art, technical characterstic of the present invention and effect:
Biological microscope illuminator based on engineering diffuser provided by the invention, by the homogeneity using engineering diffuser to improve objective table place illumination light illumination; Increased the serviceable life of illuminator as light source by use LED; This illuminator has the simple feature of structure.
Accompanying drawing explanation
Fig. 1 is the structural representation of the biological microscope illuminator based on engineering diffuser provided by the invention.
In figure, 1 is LED, and 2 is convergent lens, and 3 is engineering diffuser, and 4 is objective table.
Embodiment
The invention discloses a kind of biological microscope illuminator based on engineering diffuser, there is the simple feature of structure, controlled space distribution and the intensity profile of light beam by engineering diffuser, improve the homogeneity of objective table place illumination light illumination; By using LED as light source, increase the serviceable life of illuminator.
Technical scheme of the present invention is as follows:
1. the biological microscope illuminator based on engineering diffuser comprises: LED 1, convergent lens 2, and engineering is overflow
Beam 3, objective table 4.
2. the illuminating bundle of high strength is provided by LED 1.
3. convergent lens 2 receives and assembles illuminating bundle.
4. engineering diffuser 3 pairs of illuminating bundles control, and control space distribution and the intensity profile of illuminating bundle, make illumination
The illuminance of light beam is even.
5. biological sample to be detected placed by objective table 4.
The present invention is further described below in conjunction with the drawings and specific embodiments.
The present invention proposes a kind of biological microscope illuminator based on engineering diffuser, for the uniformity requirement of objective table place illumination light illumination, introduce the new technology of engineering diffuser; For the problem in serviceable life of illuminator, introduce LED, by reference to the accompanying drawings, be described in detail as follows.Described herein mainly based on the implementation method of the biological microscope illuminator of engineering diffuser.
Fig. 1 shows the structural representation of the biological microscope illuminator based on engineering diffuser provided by the invention, and details are as follows.
LED 1 provides the illuminating bundle of high strength, meanwhile, has longer serviceable life.
Convergent lens 2 receives and assembles the partial illumination light beam that LED 1 sends, and meanwhile, makes LED 1 and objective table 4 have Nonimage Conjugate Relations.In order to improve the light utilization efficiency of LED 1, suitably can shorten the distance of convergent lens 2 and LED 1 or increasing the size of convergent lens 2.
Engineering diffuser 3 realizes the control to illuminating bundle, the space distribution of major control illuminating bundle and intensity profile, realizes in the uniform illumination light illumination in objective table 4 place.Engineering diffuser 3 is made up of lenticule cell array, each lenticule unit forms diffuser, determined by its sag profile and position in an array, simultaneously, in order to ensure diffuser not by the impact of input beam change, and not producing diffraction phenomena, the distribution of lenticule unit is random, mainly determines according to the probability distribution function produced selected by corresponding beam shape function.The making of engineering diffuser 3 is mainly undertaken by laser ablation, and by raster scan pattern pointwise exposed photoresist layer, meanwhile, the intensity of adjustment laser beam changes photoresist depth of exposure, forms the multi-form lenticule unit being positioned at diverse location.
Controlled by engineering diffuser 3 pairs of illuminating bundles, the border circular areas with uniform illumination degree is formed at objective table 4 place, the diameter D of border circular areas depends on the angle of divergence alpha of engineering diffuser 3 and the engineering diffuser 3 distance s to objective table 4, there is mathematical relation: D=s × tan α, angle of divergence alpha is fixed values for specific engineering diffuser 3, thus, the size that distance s removes to change the border circular areas with uniform illumination degree can be adjusted.
Claims (4)
1. based on a biological microscope illuminator for engineering diffuser, it is characterized in that, comprising: LED (1), convergent lens (2), engineering diffuser (3), objective table (4); LED (1) provides the illuminating bundle of high strength; Convergent lens (2) receives and assembles illuminating bundle; Engineering diffuser (3) controls illuminating bundle, controls space distribution and the intensity profile of illuminating bundle, makes the illuminance of illuminating bundle project the upper biological sample to be detected placed of objective table (4) equably.
2. as claimed in claim 1 based on the biological microscope illuminator of engineering diffuser, it is characterized in that, engineering diffuser (3) is made up of lenticule cell array, each lenticule unit forms diffuser, being determined by its sag profile and position in an array, the distribution of lenticule unit is random.
3. as claimed in claim 1 based on the biological microscope illuminator of engineering diffuser, it is characterized in that, determine the distribution of lenticule unit according to the probability distribution function produced selected by corresponding beam shape function.
4. as claimed in claim 1 based on the biological microscope illuminator of engineering diffuser, it is characterized in that, the making of engineering diffuser (3) is realized by laser ablation, by raster scan pattern pointwise exposed photoresist layer, simultaneously, the intensity of adjustment laser beam changes photoresist depth of exposure, forms the multi-form lenticule unit being positioned at diverse location.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410746244.4A CN104459969A (en) | 2014-12-08 | 2014-12-08 | Biological microscope illuminating system based on engineering diffuser |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410746244.4A CN104459969A (en) | 2014-12-08 | 2014-12-08 | Biological microscope illuminating system based on engineering diffuser |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104459969A true CN104459969A (en) | 2015-03-25 |
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ID=52906285
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410746244.4A Pending CN104459969A (en) | 2014-12-08 | 2014-12-08 | Biological microscope illuminating system based on engineering diffuser |
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| CN (1) | CN104459969A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104808328A (en) * | 2015-05-27 | 2015-07-29 | 南通江中光电有限公司 | Novel microscope condenser |
| CN108459405A (en) * | 2018-03-23 | 2018-08-28 | 广西民族大学 | A kind of novel simple microscope |
| CN113767315A (en) * | 2019-01-18 | 2021-12-07 | Essenlix 公司 | Multi-mode lighting system |
| CN117970620A (en) * | 2024-04-01 | 2024-05-03 | 杭州海康威视数字技术股份有限公司 | Transmission illumination system and microscope |
-
2014
- 2014-12-08 CN CN201410746244.4A patent/CN104459969A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104808328A (en) * | 2015-05-27 | 2015-07-29 | 南通江中光电有限公司 | Novel microscope condenser |
| CN108459405A (en) * | 2018-03-23 | 2018-08-28 | 广西民族大学 | A kind of novel simple microscope |
| CN108459405B (en) * | 2018-03-23 | 2021-09-03 | 广西民族大学 | Novel simple microscope |
| CN113767315A (en) * | 2019-01-18 | 2021-12-07 | Essenlix 公司 | Multi-mode lighting system |
| CN113767315B (en) * | 2019-01-18 | 2024-05-24 | 上海宜晟生物科技有限公司 | Multi-mode lighting system |
| CN117970620A (en) * | 2024-04-01 | 2024-05-03 | 杭州海康威视数字技术股份有限公司 | Transmission illumination system and microscope |
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| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20150325 |