CN117965288A - Amplifying, detecting and analyzing integrated POCT device for double-fluorescence channel digital nucleic acid chip - Google Patents
Amplifying, detecting and analyzing integrated POCT device for double-fluorescence channel digital nucleic acid chip Download PDFInfo
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- CN117965288A CN117965288A CN202410179954.7A CN202410179954A CN117965288A CN 117965288 A CN117965288 A CN 117965288A CN 202410179954 A CN202410179954 A CN 202410179954A CN 117965288 A CN117965288 A CN 117965288A
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- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 37
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 37
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 37
- 238000012123 point-of-care testing Methods 0.000 title claims abstract description 21
- 238000001514 detection method Methods 0.000 claims abstract description 48
- 238000010438 heat treatment Methods 0.000 claims abstract description 44
- 230000003287 optical effect Effects 0.000 claims abstract description 21
- 238000005192 partition Methods 0.000 claims abstract description 16
- 239000003086 colorant Substances 0.000 claims abstract description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 31
- 229910002804 graphite Inorganic materials 0.000 claims description 30
- 239000010439 graphite Substances 0.000 claims description 30
- 238000004458 analytical method Methods 0.000 claims description 16
- 230000003321 amplification Effects 0.000 claims description 13
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 13
- 230000009977 dual effect Effects 0.000 claims description 8
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 3
- 239000002131 composite material Substances 0.000 claims description 3
- 229920000642 polymer Polymers 0.000 claims description 3
- 239000000741 silica gel Substances 0.000 claims description 3
- 229910002027 silica gel Inorganic materials 0.000 claims description 3
- 238000012544 monitoring process Methods 0.000 claims description 2
- 239000012528 membrane Substances 0.000 claims 1
- 230000005284 excitation Effects 0.000 description 6
- 238000000034 method Methods 0.000 description 5
- 239000000523 sample Substances 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 238000001914 filtration Methods 0.000 description 3
- 230000009089 cytolysis Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000012921 fluorescence analysis Methods 0.000 description 2
- 244000052769 pathogen Species 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 108020000946 Bacterial DNA Proteins 0.000 description 1
- 206010011409 Cross infection Diseases 0.000 description 1
- WHXSMMKQMYFTQS-UHFFFAOYSA-N Lithium Chemical compound [Li] WHXSMMKQMYFTQS-UHFFFAOYSA-N 0.000 description 1
- 206010029803 Nosocomial infection Diseases 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 239000000443 aerosol Substances 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000002306 biochemical method Methods 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 239000002041 carbon nanotube Substances 0.000 description 1
- 229910021393 carbon nanotube Inorganic materials 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 108091036078 conserved sequence Proteins 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000007847 digital PCR Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 229910021389 graphene Inorganic materials 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 238000011901 isothermal amplification Methods 0.000 description 1
- 239000006101 laboratory sample Substances 0.000 description 1
- 229910052744 lithium Inorganic materials 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 230000000474 nursing effect Effects 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000007859 qualitative PCR Methods 0.000 description 1
- 238000003753 real-time PCR Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000002834 transmittance Methods 0.000 description 1
Abstract
The invention relates to an amplifying, detecting and analyzing integrated POCT device for a double-fluorescent channel digital nucleic acid chip, which comprises a rectangular box-shaped shell, wherein an upper partition board and a lower partition board which are arranged in the shell at an upper-lower parallel interval are arranged in the shell, and the interior of the shell is divided into a detection cavity, a heating cavity and a power supply cavity from top to bottom; a reading hole is formed in the top plate of the shell; the reading hole is provided with a reading module which can be connected with the reading module by using a mobile phone to read information of the nucleic acid chip placed in the detection cavity; the bottom surface of the top plate is provided with a switchable filter module, so that different optical filters can be aligned to the reading holes; at least two lamp strips capable of generating light sources with different colors are arranged on the inner side wall of the detection cavity; a heating film is arranged in the heating cavity; a battery is arranged in the power supply cavity; the battery is electrically connected with the heating film. The invention can improve the detection efficiency, reduce the use cost, is convenient to carry and is worth popularizing.
Description
Technical Field
The invention relates to medical detection equipment, in particular to a POCT (point-of-CARE TESTING instant detection) device, and specifically relates to an integrated POCT device for amplification, detection and analysis of a double-fluorescent-channel digital nucleic acid chip.
Background
Nucleic acid detection is a biochemical technique for detecting specific nucleic acids in biological samples, and has been widely used for diagnosis of infection, gene detection, and forensic identification of viruses and bacteria. By rapid and timely nucleic acid detection, the presence of pathogens can be identified early. Current PCR technology has evolved from qualitative PCR and quantitative PCR to the latest digital PCR. The digital nucleic acid detection of pathogens requires specialized laboratories and operators, and has high technical requirements. Meanwhile, the centralized laboratory detection requires a large number of sampling personnel to take time and labor to collect samples, long-time sample transportation and preservation are required, and the problem of cross infection in the laboratory sample adding process is solved.
The existing POCT (point-of-care testing) device mainly comprises a sensing component, a signal acquisition and processing system and an embedded power supply unit. The sensing assembly mostly adopts test tubes or lateral chromatographic test paper, for example SHERLOCK and DETECTR, paper lateral chromatographic test paper suitable for bedside use is needed to be used in a matched mode for reading, and detection results are judged according to whether the test tubes emit light or whether positive strips appear on the test paper. But it has the following problems:
1) Very complex and precise optical signal filtering and signal amplifying processes are often required, and expensive optical signal sensing elements or devices are required, so that the problems of insufficient integration level, large number of light sources and detectors, large equipment and high cost exist, and the universality of the application of the optical signal filtering and signal amplifying device is limited.
2) Most of the prior optical elements and the prior heating elements are adopted, only one color of laser and optical filter is needed, so that the amplification and the fluorescence analysis are needed to be carried out separately, aerosol pollution and sample loss are easy to cause, dual fluorescence simultaneous detection cannot be achieved, and the efficiency is low.
Accordingly, there is a need for improvements in the art to better meet the needs of use.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides the POCT device for integrating amplification, detection and analysis of the double-fluorescence channel digital nucleic acid chip, which is small and portable in equipment, has double channels of double fluorescence and double filters, can flexibly change fluorescence of a detection object according to detection requirements, and greatly improves detection efficiency.
The technical scheme of the invention is as follows:
The POCT device comprises a rectangular box-shaped shell, wherein an upper partition board and a lower partition board which are arranged in the shell and are spaced in parallel from top to bottom are arranged in the shell, and the interior of the shell is divided into a detection cavity, a heating cavity and a power supply cavity from top to bottom; the top plate of the shell is detachable, and is provided with a reading hole which is internally and externally communicated; a cylindrical reading module corresponding to the reading hole is arranged on the top surface of the top plate, and the mobile phone can be used for connecting the reading module to read information of the double-fluorescent-channel digital nucleic acid chip placed in the detection cavity; the bottom surface of the top plate is provided with a switchable filter module; the filter module comprises at least two different optical filters; the optical filters can be aligned with the reading holes respectively; at least two lamp strips capable of generating light sources with different colors are arranged on the inner side wall of the detection cavity; a heating film is arranged in the heating cavity; a battery is arranged in the power supply cavity; the battery is electrically connected with the heating film.
Further, the edge of the top plate is provided with a downward bulge; the upper end of the side wall of the shell is provided with a step-shaped groove; the protrusions and the grooves correspond to each other, so that the top plate can be conveniently positioned and installed.
Further, the reading module comprises a focusing cylinder, a focusing hand wheel and a graphite tube; the focusing cylinder, the focusing hand wheel and the graphite tube are all cylindrical with two open ends; the focusing cylinder is sleeved on the focusing hand wheel through threads; the graphite tube is made of graphite, the lower end of the graphite tube is fixed on the reading hole, and the upper end of the graphite tube is suspended; the focusing hand wheel is movably sleeved on the graphite tube, and the bottom of the focusing hand wheel is provided with a flange; an ocular lens is arranged at the upper end of the focusing cylinder; an objective lens is arranged at the lower end of the graphite tube.
Further, the device also comprises a connecting block; the connecting block is cylindrical with two open ends, the lower end of the connecting block is connected with the reading hole, and the upper end of the connecting block is connected with the lower end of the graphite tube.
Further, the filter module comprises a bracket; the bracket is disc-shaped, and the center of the bracket is connected with the bottom surface of the top plate through a rotating shaft so that the bracket can rotate around the device; the bracket is provided with mounting holes arranged along the circumferential direction of the bracket; the optical filter is embedded in the mounting hole.
Further, the optical filter is a narrow-band optical filter and is symmetrically arranged at the center of the bracket.
Further, a tooth-shaped structure is arranged on the outer edge of the bracket; the diameter of the bracket is larger than the width of the top plate, so that part of the outer edges of the bracket are arranged outside the two sides of the top plate; the lateral wall in detection chamber is equipped with the breach, with the support suits.
Further, the lamp strips are arranged on the long sides of the monitoring cavity in a mutually opposite mode, and are electrically connected with the battery after being respectively switched through the lamp strips.
Further, the thickness of the heating film is equivalent to the height of the heating cavity; the upper partition plate is made of heat-conducting silica gel and is attached to the heating film.
Further, the heating film is made of a polymer PTC composite material, is coupled with the thermocouple, and is electrically connected with the battery through the heating switch.
The invention has the beneficial effects that:
1. the invention has the advantages of small structure, convenient carrying, double fluorescence and double filtration, flexible change of fluorescence of the detection object according to the detection requirement, and convenient popularization and use.
2. The invention can simultaneously complete nucleic acid amplification and result analysis without taking out a chip, thereby improving detection efficiency.
3. The invention can read information from the detection chip after the mobile phone is connected with the reading module, thereby avoiding the prior expensive and heavy analysis instrument and reducing the use cost.
Drawings
Fig. 1 is a schematic view of the appearance of the present invention.
Fig. 2 is a schematic longitudinal section of the present invention.
Fig. 3 is a schematic structural view of the filter module of the present invention.
Fig. 4 is a schematic structural view of the reading module of the present invention.
Fig. 5 is a schematic view of the lamp strip installation of the present invention.
Wherein, 1-shell; 101-top plate; 102-a detection chamber; 103-upper partition; 104-a lower partition; 105-a power supply cavity; 106-heating the cavity; 107-connecting blocks; 2-a reading module; 201-eyepiece; 202, focusing a barrel; 203-limiting rings; 204-focusing handwheel; 205-graphite tube; 206-an objective lens; 3-a filter module; 301-a bracket; 302-mounting holes; 303-rotating shaft; 304-an optical filter; 4-a lamp strip switch; 5-a heating switch; 6-cell; 7-heating the film; 8-nucleic acid chip; 9-lamp strip.
Detailed Description
The invention is further described below with reference to the drawings and examples.
As shown in fig. 1 to 5.
An amplifying, detecting and analyzing integrated POCT device for a double-fluorescent channel digital nucleic acid chip comprises a shell 1, a reading module 2, a filter module 3 and the like.
The shell 1 is rectangular box-shaped, and is internally provided with an upper partition plate 103 and a lower partition plate 104 which are spaced in parallel from top to bottom, and the interior of the shell is divided into a detection cavity 102, a heating cavity 106 and a power supply cavity 105 from top to bottom. The detection cavity 102 is internally provided with a lamp band 9 and can be used for placing a detected nucleic acid chip 8. The heating cavity 106 is internally provided with a heating film 7. A battery 6 is arranged in the power cavity 105.
The top plate 101 of the shell 1 is detachable, a reading hole which is internally and externally communicated is arranged on the top plate, and a downward bulge is arranged at the edge of the reading hole. Meanwhile, a step-shaped groove is formed in the upper end of the side wall of the shell 1. Moreover, the protrusions correspond to the grooves, and the size of the protrusions is equivalent, so that the protrusions can be embedded into the grooves, and the top plate 101 can be positioned and disassembled conveniently.
The number of the lamp strips 9 is two, and the two lamp strips can respectively emit excitation light with the wavelength of 494nm and excitation light with the wavelength of 518 nm. The lamp strips 9 are oppositely arranged on the inner wall of the long side of the detection cavity 102 and are respectively electrically connected with the battery after passing through the lamp strip switch 4. The lamp strip switch 4 is a one-pole double-throw switch, and has three gear positions of 0, 1 and 2, which respectively correspond to the excitation light with the wavelength of 494nm and the excitation light with the wavelength of 518 nm.
The nucleic acid chip 8 is a double-fluorescence channel digital nucleic acid chip, and can generate different fluorescence effects under the illumination of different wavelengths. The digital nucleic acid chip provided by zhen quasi biotechnology Co., ltd can be selected.
The heating film 7 is made of a polymer PTC composite material of graphene and carbon nano tubes, and can keep relatively stable temperature under external stable voltage. The thickness of the heating film 7 corresponds to the height of the heating chamber 106, so that it can be attached to the upper partition 103. The upper partition 103 is made of heat-conductive silica gel, and has a thickness of about 5mm, so that it has good heat-conductive properties. The heating film 7 is further coupled with a thermocouple and is electrically connected with the battery 6 through the heating switch 5, so that the heating film 7 can be controlled to be opened and closed through the heating switch 5, a thermal signal can be converted into an electric signal through the thermocouple, and the reaction temperature on a chip in the isothermal amplification process is recorded.
The battery 6 is a lithium battery, and the voltage is 9V.
The reading module 2 comprises a focusing cylinder 202, a focusing hand wheel 204 and a graphite tube 205. The focusing barrel 202, the focusing hand wheel 204 and the graphite tube 205 are all cylindrical with two open ends. An eyepiece 201 is arranged at the upper end of the focusing cylinder 202, and is sleeved on the focusing hand wheel 204 through threads, and the focusing cylinder 202 can be driven to move up and down through rotation of the focusing hand wheel 204, so that the adjustment of the focal length of the eyepiece 201 is realized. Preferably, a flange is provided at the bottom of the focusing hand wheel 204, so as to facilitate the rotation operation. Meanwhile, the limiting ring 203 is sleeved on the focusing hand wheel 204, so that the focusing hand wheel can be prevented from being excessively rotated to damage the ocular lens.
The height of the graphite tube 205 is equal to that of the focusing hand wheel 204, the lower end of the graphite tube is provided with an objective lens 206 and is fixed on the reading hole through the connecting block 107, and the upper end of the graphite tube is suspended. The connecting block 107 is cylindrical with two open ends, the lower end of the connecting block is connected with the reading hole, and the upper end of the connecting block is connected with the lower end of the graphite tube 205, so that the reading module is convenient to install. The focusing hand wheel 204 is movably sleeved on the graphite tube 205. Meanwhile, a flange is also arranged at the lower end of the graphite tube 205, so that the focusing hand wheel 204 can be supported, and the focusing hand wheel 204 and the graphite tube 205 can rotate mutually. The graphite tube 205 is made of graphite, and can absorb light, so as to prevent light reflection from affecting the detection result. Preferably, the focal length of the eyepiece 201 is 10mm, the focal length of the objective lens 206 is 10mm, and the distance between the eyepiece 201 and the objective lens 206 can be adjusted between 30mm and 60mm by rotating the focusing handwheel.
The filter module 3 comprises a holder 301. The support 301 has a disk shape, and its center is connected to the bottom surface of the top plate 101 through a rotation shaft 303 so that it can rotate around the rotation shaft 303. The holder 301 is provided with mounting holes 302 provided along the circumferential direction thereof, and the filter 304 is fitted into the mounting holes 302. The number of the mounting holes 302 is two, and the mounting holes are symmetrically arranged. The optical filters 304 are a BP518 narrow-band optical filter and a BP556 narrow-band optical filter, the central wavelengths of which are 518nm < + > -5nm and 556nm < + > -5nm respectively, the half bandwidth is 15nm, and the peak transmittance is more than 90%. Preferably, the diameter of the support 301 is larger than the width of the top plate 101, and a notch is provided on the side wall of the detection chamber 102. The notch is adapted to the bracket 301, so that a portion of the outer edge of the bracket 301 is disposed outside the two sides of the top plate 101, and is convenient for rotation.
Further, the outer edge of the bracket 301 is provided with a tooth-shaped structure, which is convenient for rotation operation. The rotating shaft 303 is disposed on one side of the reading hole, and is adapted to the distance between the center of the reading hole and the position of the mounting hole, so that any one of the optical filters 304 may rotate along with the bracket 301 to face the reading hole.
The working process of the invention is as follows:
the sample to be tested and the reagent are split-filled into the formed chip chamber with fixed volume to form individual microdroplets, and sealing is completed, so that the nucleic acid is uniformly divided into a plurality of independent reaction units.
The top plate is opened, and the nucleic acid chip is placed in the center of the upper partition plate in the detection chamber so that the center of the nucleic acid chip is aligned with the center of the detection chamber, so that the whole nucleic acid chip can be seen when observed.
The top plate is covered, and the heating switch is turned on, so that the heating film heats. The PTC resistance value in the heating film increases along with the temperature rise, and the magnitude of current is controlled by a signal fed back by a temperature sensor, so that the heating value of the internal resistance of the heating film is adjusted, and the heating film is kept in a constant temperature state of about 40 ℃. The nucleic acid chip is heated by the heating film through the upper partition board, so that the nucleic acid chip is incubated at 40 ℃ and subjected to bacterial lysis and RPA reaction. After lysis, the bacterial DNA is released and the conserved sequence is detected using double stranded RPA with fluorescent probes.
After RPA, the heating switch was turned off and the light band switch was turned on to detect fluorescence of both amplified markers. Observe FAM channels: opening 494nm excitation light source lamp band, rotating the bracket to align the center of the optical filter of BP518 with the center of the nucleic acid chip; observation of the HEX channel: the 535nm excitation light source lamp band is turned on, and the bracket is rotated, so that the center of the optical filter of BP556 is aligned with the center of the nucleic acid chip.
Then, the mobile phone camera is aligned with the ocular, the focusing hand wheel is rotated to focus, and the fluorescent effect of the chip is shot. Subsequently, the smartphone-based fluorescence analysis application was opened and the initial nucleic acid concentration in the sample was calculated by poisson statistics based on the number of positive and negative droplets, respectively.
In the past, when gold standard is used for analysis, species and mutation can be determined usually in a long time, and when the method is used for analysis, automatic sample analysis reading can be completed in less than 1 hour, so that the detection efficiency is greatly improved, and the use cost is greatly reduced. Moreover, the invention has small volume and convenient carrying, and can be an ideal tool for nursing point setting such as hospital admission screening and the like.
The invention is not related in part to the same as or can be practiced with the prior art.
Claims (10)
1. The POCT device for amplifying, detecting and analyzing the double-fluorescent channel digital nucleic acid chip comprises a shell, and is characterized in that the shell is rectangular and box-shaped, an upper partition board and a lower partition board which are arranged in the shell and are parallel to each other at intervals are arranged in the shell, and the interior of the shell is divided into a detection cavity, a heating cavity and a power supply cavity from top to bottom; the top plate of the shell is detachable, and is provided with a reading hole which is internally and externally communicated; a cylindrical reading module corresponding to the reading hole is arranged on the top surface of the top plate, and the mobile phone can be used for connecting the reading module to read information of the double-fluorescent-channel digital nucleic acid chip placed in the detection cavity; the bottom surface of the top plate is provided with a switchable filter module; the filter module comprises at least two different optical filters; the optical filters can be aligned with the reading holes respectively; at least two lamp strips capable of generating light sources with different colors are arranged on the inner side wall of the detection cavity; a heating film is arranged in the heating cavity; a battery is arranged in the power supply cavity; the battery is electrically connected with the heating film.
2. The integrated POCT device for amplification, detection and analysis of dual fluorescent channel digital nucleic acid chips of claim 1, wherein the edge of the top plate is provided with a downward protrusion; the upper end of the side wall of the shell is provided with a step-shaped groove; the protrusions and the grooves correspond to each other, so that the top plate can be conveniently positioned and installed.
3. The integrated POCT device for amplification, detection and analysis of double-fluorescence channel digital nucleic acid chips according to claim 1, wherein the reading module comprises a focusing cylinder, a focusing hand wheel and a graphite tube; the focusing cylinder, the focusing hand wheel and the graphite tube are all cylindrical with two open ends; the focusing cylinder is sleeved on the focusing hand wheel through threads; the graphite tube is made of graphite, the lower end of the graphite tube is fixed on the reading hole, and the upper end of the graphite tube is suspended; the focusing hand wheel is movably sleeved on the graphite tube, and the bottom of the focusing hand wheel is provided with a flange; an ocular lens is arranged at the upper end of the focusing cylinder; an objective lens is arranged at the lower end of the graphite tube.
4. The integrated POCT device for amplification, detection, and analysis of dual fluorescent channel digital nucleic acid chips of claim 3, further comprising a connection block; the connecting block is cylindrical with two open ends, the lower end of the connecting block is connected with the reading hole, and the upper end of the connecting block is connected with the lower end of the graphite tube.
5. The integrated POCT device for amplification, detection, analysis of dual fluorescent channel digital nucleic acid chips of claim 1, wherein the filter module comprises a support; the bracket is disc-shaped, and the center of the bracket is connected with the bottom surface of the top plate through a rotating shaft so that the bracket can rotate around the device; the bracket is provided with mounting holes arranged along the circumferential direction of the bracket; the optical filter is embedded in the mounting hole.
6. The integrated POCT device for amplification, detection and analysis of dual fluorescent channel digital nucleic acid chips of claim 5, wherein the filter is a narrowband filter, and is arranged symmetrically about the center of the support.
7. The integrated POCT device for amplification, detection and analysis of double-fluorescence channel digital nucleic acid chip according to claim 5, wherein the outer edge of the bracket is provided with a tooth-shaped structure; the diameter of the bracket is larger than the width of the top plate, so that part of the outer edges of the bracket are arranged outside the two sides of the top plate; the lateral wall in detection chamber is equipped with the breach, with the support suits.
8. The integrated POCT device for amplification, detection and analysis of dual fluorescent channel digital nucleic acid chips of claim 1, wherein the light strips are disposed opposite each other on the long side of the monitoring chamber and electrically connected to the battery after being turned on and off by the light strips.
9. The integrated POCT device for amplification, detection, and analysis of dual fluorescent channel digital nucleic acid chips of claim 1, wherein the thickness of the heating film is comparable to the height of the heating cavity; the upper partition plate is made of heat-conducting silica gel and is attached to the heating film.
10. The integrated POCT device for amplification, detection and analysis of dual fluorescent channel digital nucleic acid chip of claim 1, wherein the heating membrane is made of a polymer PTC composite material, and is electrically connected to the battery via a heating switch after being coupled to a thermocouple.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202410179954.7A CN117965288A (en) | 2024-02-18 | 2024-02-18 | Amplifying, detecting and analyzing integrated POCT device for double-fluorescence channel digital nucleic acid chip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202410179954.7A CN117965288A (en) | 2024-02-18 | 2024-02-18 | Amplifying, detecting and analyzing integrated POCT device for double-fluorescence channel digital nucleic acid chip |
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| Publication Number | Publication Date |
|---|---|
| CN117965288A true CN117965288A (en) | 2024-05-03 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202410179954.7A Pending CN117965288A (en) | 2024-02-18 | 2024-02-18 | Amplifying, detecting and analyzing integrated POCT device for double-fluorescence channel digital nucleic acid chip |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN117965288A (en) |
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- 2024-02-18 CN CN202410179954.7A patent/CN117965288A/en active Pending
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