CN117929771A

CN117929771A - Sample adding device

Info

Publication number: CN117929771A
Application number: CN202410108179.6A
Authority: CN
Inventors: 陶瑞; 谢红梅
Original assignee: Suzhou Bangqi Biotechnology Co ltd
Current assignee: Suzhou Bangqi Biotechnology Co ltd
Priority date: 2024-01-26
Filing date: 2024-01-26
Publication date: 2024-04-26

Abstract

The application belongs to the field of medical equipment, and discloses a sample adding device which comprises a mechanical arm module, a reagent needle module and a mixing disc module, wherein the mechanical arm module is used for placing a reaction cup in the mixing disc, and sucking a sample from a sample rack and adding the sample into the reaction cup; the reagent needle module is used for sucking the reagent from the reagent bin and adding the reagent into the reaction cup in the mixing disc; the mixing disc module is used for loading a solution formed by a sample and a reagent and achieving the purpose of mixing the solution; the application provides a sample adding device with low cost and high precision, solves the challenges of accurate microsampling in the prior art, can meet the strict requirements of a full-automatic flow type fluorescent immunodetection instrument on sample adding precision, and ensures that reaction liquid is fully mixed through a unique mixing sleeve design, thereby improving the accuracy of detection results.

Description

Sample adding device

Technical Field

The application relates to the field of medical instruments, in particular to a sample adding device.

Background

The full-automatic flow type fluorescence immunoassay instrument is a high-efficiency and high-flux detection device based on the flow type fluorescence technology, and is mainly used for rapidly and accurately detecting target substances in biological samples. The technology integrates a plurality of latest technologies such as fluorescent coding microspheres, laser analysis, application of fluidics, high-speed digital signal processing and the like, and has the characteristics of high flux, high sensitivity and high specificity.

The core components of the full-automatic flow type fluorescence immunoassay instrument comprise:

sample adding device: is responsible for adding the sample to be tested to the instrument. The device generally comprises a mechanical arm module, a reagent needle module, a mixing disc module and the like, and can automatically add samples and reagents into a reaction cup.

Reagent preservation device: is an important component of the instrument for storing various reagents. The reagent cartridge is typically designed at a constant temperature to ensure stability and effectiveness of the reagent. In addition, the reagent bin also needs to have functions of dust prevention, moisture prevention and the like so as to protect the reagent from pollution and deterioration.

A reaction chamber: is the core part of the instrument for carrying out immune reaction and detection of fluorescent signals.

The detection system comprises: including optical systems and electronic control systems. The optical system is used for exciting and detecting fluorescent signals, and the electronic control system is responsible for controlling all parts of the instrument to ensure the normal operation of the instrument.

A data processing system: is responsible for processing and analyzing the detected data and outputting the results to the user.

Auxiliary component: including power supplies, display screens, operator interfaces, etc.

In the full-automatic flow type fluorescence immunoassay instrument, the sample adding device is a core component, and the accuracy directly determines the reliability of the detection result. In general, a sample size of 1. Mu.L is required for clinical detection, and then 19. Mu.L of the reagent is added for uniform reaction. However, due to technical limitations, it is difficult to achieve accurate microsampling with current sample loading devices, and particularly for low volumes of 1 μl, there is often a large bias in practical detection.

In order to solve the problem, the application designs the accurate sample adding device with low cost, which can meet the requirement of the full-automatic flow type fluorescence immunoassay instrument on the sample adding precision, thereby being beneficial to improving the accuracy and the reliability of clinical examination and providing more reliable basis for medical diagnosis and treatment.

Disclosure of Invention

The application aims to provide a sample adding device with low cost and high precision, which solves the challenges of accurate microsampling in the prior art, can meet the strict requirements of a full-automatic flow type fluorescence immunoassay instrument on sample adding precision, and ensures that reaction liquid is fully mixed through a unique mixing sleeve design, thereby improving the accuracy of detection results.

In order to effectively solve the technical problems, the aim of the application is realized by the following technical scheme.

According to a first aspect of the present invention, there is provided a sample application device comprising

The mechanical arm module is used for placing the reaction cup in the mixing disc; for sucking up a sample from a sample holder and adding it to the cuvette;

a reagent needle module for sucking the reagent from the reagent bin and adding the reagent into the reaction cup;

the mixing disc module is used for loading a solution formed by a sample and a reagent and achieving the purpose of mixing the solution;

in a specific embodiment of the application, the mechanical arm module comprises a cuvette holder for placing a cuvette in a cuvette hole of a mixing tray.

In a specific embodiment of the application, the reagent needle module comprises a reagent needle, a needle holder, a reagent needle driving device.

Further, the reagent needle driving device comprises a rotary motion motor for driving the reagent needle to move circumferentially.

Further, the reagent needle driving device comprises an up-and-down motion motor for driving the reagent needle to move up and down.

In a specific embodiment of the present application, the mixing disc module includes a mixing disc and a reaction cup hole disposed on the mixing disc.

Further, the mixing disc module comprises a mixing disc rotating device for driving the mixing disc to rotate and a mixing device for uniformly mixing the reaction cups.

Further, the mixing disc rotating device comprises a mixing disc rotating motor and a rotating angle code disc.

Further, the mixing device comprises a mixing sleeve, a mixing sleeve up-and-down motion motor, a screw rod, a mixing sleeve limiting device and a mixing sleeve rotating motor.

In a preferred embodiment of the present application, the mixing sleeve rotating motor is connected with the mixing sleeve.

According to a second aspect of the present invention, there is provided an in vitro diagnostic device comprising: and a sample adding device.

According to a third aspect of the present invention, there is provided a fully automatic flow fluorescent immunoassay instrument comprising: reagent preservation device, sample adding device, incubation device, optical detection system, software system; the sample adding device is used for adding reagents and samples into the reaction cup; the incubation device is used for precisely controlling the temperature of the reaction, so that the reaction is ensured to be more sufficient; the optical detection system is used for detecting the coding and fluorescence intensity of the microsphere; the software system performs statistical analysis on the detection signals collected by the optical detection system.

The advantages of the application mainly include the following aspects:

The sample adding device is provided with the mixing disc module, the reagent needle module and the mechanical arm module, wherein the mixing disc module can fully mix the reaction liquid in a short time in a rotating and vibrating mode, so that the clinical detection efficiency can be improved, and the error caused by uneven mixing can be reduced; the reagent needle module can freely realize lifting and rotating functions to finish the process of extracting the reagent from the reagent bottle, moving the reagent to the upper part of the reaction cup and then lowering the reagent needle to add the reagent to the reaction cup; the mechanical arm module can automatically complete the placement and extraction of the reaction cup, and the step and time cost of manual operation are greatly reduced. According to the application, the sample adding amount is accurately controlled, so that the waste of reagents and samples is reduced, and the experimental cost and the influence on the environment are reduced. In conclusion, the sample adding device provided by the application has the advantages of low cost, high precision, full mixing and the like, and can meet the requirement of a full-automatic flow type fluorescence immunoassay instrument on sample adding precision. By the application of the device, the accuracy and the reliability of the detection result can be improved, and the device provides powerful support for biomedical research.

Drawings

FIG. 1 is a schematic perspective view of a sample application device of the present application;

FIG. 2 is another perspective view of the sample application device of the present application;

FIG. 3 is a schematic plan view of the sample application device of the present application;

FIG. 4 is a schematic view of a reagent needle module according to the present application;

FIG. 5 is a schematic diagram of a mixing disk module according to the present application;

FIG. 6 is a schematic view of another view of a mixing disk module according to the present application;

FIG. 7 is a perspective view of a mixing sleeve;

fig. 8 is a schematic diagram of a motor rotating device of the mixing sleeve, the reaction cup and the mixing sleeve.

FIG. 9 is a schematic perspective view of a sample application device according to the present application;

in the figure, a 1-mechanical arm module and a 101-reaction cup gripper are arranged; 102-a pipetting pump;

2-reagent needle module, 201-reagent needle, 202-needle holder, 203-rotary motion motor, 204-first mounting plate, 205-spline shaft, 206-first driving pulley, 207-first driven pulley, 208-first timing belt, 209-up-down motion motor, 210-first slide rail, 211-first side plate, 212-second mounting plate, 213-second driving pulley, 214-second driven pulley, 215-second timing belt, 216-third mounting plate;

The device comprises a 3-mixing disc module, 301-reaction cups, 302-mixing discs, 303-mixing disc rotating motors, 304-fourth mounting plates, 305-motor supports, 306-third driving pulleys, 307-third driven pulleys, 308-third synchronous belts, 309-supporting shafts, 310-placing grooves, 311-mixing sleeves, 312-mixing sleeve up-and-down motion motors, 313-mixing sleeve rotating motors, 314-second sliding rails, 315-first supporting frames, 316-fifth mounting plates, 317-screw rods, 318-sliding blocks, 319-second supporting frames, 320-L-shaped supports, 321-reaction cup baffles and 322-mixing bosses.

Detailed Description

The present application will be described in further detail with reference to the drawings and examples, in order to make the objects, technical solutions and advantages of the present application more apparent. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the application.

In the description of the present application, it should be understood that the terms "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "circumferential", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are merely for convenience in describing the present application and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present application.

Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include at least one such feature. In the description of the present application, the meaning of "plurality" means at least two, for example, two, three, etc., unless specifically defined otherwise.

In the present application, unless explicitly specified and limited otherwise, the terms "mounted," "connected," "secured," and the like are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; either directly or indirectly, through intermediaries, or both, may be in communication with each other or in interaction with each other, unless expressly defined otherwise. The specific meaning of the above terms in the present application can be understood by those of ordinary skill in the art according to the specific circumstances.

In the present application, unless expressly stated or limited otherwise, a first feature "up" or "down" a second feature may be the first and second features in direct contact, or the first and second features in indirect contact via an intervening medium. Moreover, a first feature being "above," "over" and "on" a second feature may be a first feature being directly above or obliquely above the second feature, or simply indicating that the first feature is level higher than the second feature. The first feature being "under", "below" and "beneath" the second feature may be the first feature being directly under or obliquely below the second feature, or simply indicating that the first feature is less level than the second feature.

In the description of the present specification, a description referring to terms "one embodiment," "some embodiments," "examples," "specific examples," or "some examples," etc., means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the present application. In this specification, schematic representations of the above terms are not necessarily directed to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, the different embodiments or examples described in this specification and the features of the different embodiments or examples may be combined and combined by those skilled in the art without contradiction.

It should be further noted that, in the embodiments of the present application, the same reference numerals denote the same components or the same parts, and for the same parts in the embodiments of the present application, reference numerals may be given to only one of the parts or the parts in the drawings, and it should be understood that, for other same parts or parts, the reference numerals are equally applicable.

In the description of the present application, it should be noted that the term "and/or" is merely an association relationship describing an association object, and indicates that three relationships may exist, for example, a and/or B may indicate: a exists alone, A and B exist together, and B exists alone.

For convenience of description, the whole frame is abstracted into a rectangular plane, the long side direction is transverse, the short side direction is longitudinal, and in addition, the transverse direction is set to be an X axis, the longitudinal direction is set to be a Y axis, and the vertical direction of the frame is set to be a Z axis, and it should be clear that this designation is for convenience of description only and is not a limitation of structure.

In order to more conveniently illustrate the sample loading device provided by the embodiment of the present application, by way of example, but not by way of limitation, the technical scheme of the present application will be described in detail below.

The sample loading device provided by the present application will now be described in detail with reference to fig. 1 to 8.

Referring to fig. 1, the application designs a sample adding device, which comprises a mechanical arm module 1, a reagent needle module 2 and a mixing disc module 3. The mechanical arm module 1 can automatically complete the placement of the reaction cup and the addition of the sample, so that the steps of manual operation and the time cost are greatly reduced; the reagent needle module 2 can freely realize lifting and rotating functions, and the process of adding the reagent into the reaction cup 301 is completed; the mixing disk module 3 is configured to sufficiently mix the reaction solution in a short time by means of rotation, vibration, or the like.

Specifically, the robot arm module 1 includes a cuvette gripper 101 and a transfer pump 102. The cuvette holder 1 has a clamping part adapted to the shape of the cuvette, and can accurately and firmly hold the cuvette and place it in the cuvette hole on the mixing tray 302. The pipette can firstly suck the gun head, then move to the upper part of the blood collection tube to suck the sample and then add the sample into the reaction cup.

More specifically, the mechanical arm module 1 comprises a driving device and a control system, and can realize movement in the X-axis, Y-axis and Z-axis directions so as to ensure that the reaction cup is accurately placed at a designated position on the mixing disc, and the liquid transfer pump can accurately suck the gun head and can suck samples from the sample tube and add the samples into the reaction cup.

In a specific embodiment of the present application, the reagent needle module 2 comprises a reagent needle 201, a needle holder 202, a reagent needle driving means. Wherein one end of the needle holder 202 is connected with the reagent needle 201 and the other end is connected with the reagent needle driving device. Specifically, the needle head of the reagent needle 201 is vertically disposed downward along the Z-axis direction, and the needle holder 202 is perpendicular to the reagent needle 201.

Wherein, in order to realize the lift and rotation function of reagent needle 202, reagent needle drive arrangement includes rotary motion motor 203, first mounting panel 204, first belt drive, the integral key shaft 205 that is used for driving reagent needle circumferential motion, specifically, the integral key shaft 205 upper end is connected with the one end of needle holder, and the lower extreme passes first belt drive and first mounting panel 204 downwardly extending, rotary motion motor 203 installs in the back of first mounting panel 204, and wherein rotary motion motor's output shaft is vertically upwards. The rotary motor 203 drives the spline shaft 205 to rotate through the first driving device, and further drives the needle holder to circumferentially rotate around the spline shaft. Further, the first driving device includes a first driving pulley 206, a first driven pulley 207 and a first synchronous belt 208, the first driving pulley is connected with the output shaft of the rotary motion motor, the first driven pulley is sleeved outside the spline shaft, and the first driven pulley is connected with the first driving pulley through the first synchronous belt.

The reagent needle driving device further comprises an up-down motion motor 209, a first sliding rail 210 and a second driving device, wherein the up-down motion motor is used for driving the reagent needle to move up and down. The up-down motion motor is mounted on the first side plate 211, the upper end of the first side plate 211 is vertically fixed with the first mounting plate 204, and the lower end is vertically fixed with the second mounting plate 212. The output shaft of the up-down motion motor is connected with a second driving device, and the second driving device comprises a second driving belt pulley 213, a second driven belt pulley 214 and a second synchronous belt 215.

Specifically, the second driving pulley 213 is sleeved on the output shaft of the up-down motion motor 209, the second driven pulley 214 is mounted on the first side plate 211 through a second driven pulley seat, the second driven pulley 214 is connected with the second driving pulley through a second synchronous belt, the second synchronous belt is fixedly connected with a third mounting plate 216, the other end of the third mounting plate 216 is connected with the spline shaft 205, and a ball bearing is arranged between the spline shaft 205 and the third mounting plate 216. In an embodiment of the present application, to further facilitate the up-and-down movement of the spline shaft, a first sliding rail 210 is disposed between the first mounting plate 204 and the second mounting plate 212, and a third mounting plate 216 is fixedly connected to the first sliding rail 210. In the present application, the up-down movement motor 209 drives the third mounting plate 216 to move up and down along the first sliding rail 210 through the second driving device, so as to drive the reagent needle 201 to move up and down.

In the in-service use process of the reagent needle module, the motor cooperatively works, so that accurate movement of the reagent needle and accurate suction and placement of the reagent are realized, specifically, the reagent needle is driven by the reagent needle rotating motor to move to the upper side of the reagent bin, the reagent needle is driven by the reagent needle up-and-down movement motor to move downwards to suck the reagent, after the reagent is finished, the reagent needle up-and-down movement motor is reset, the reagent needle is driven by the reagent needle rotating motor to move to the upper side of the reaction cup, and the reagent is placed in the reaction cup through the reagent needle up-and-down movement motor to drive the reagent needle to move downwards.

In one embodiment of the present application, mixing disk module 3 includes mixing disk 302 and reaction cup holes provided in the mixing disk. In order to realize more accurate microscale sample adding, the reaction cup holes are provided with a first group and a second group, wherein the first group is positioned on the inner side of the second group, namely the first group is an inner ring reaction cup, and the second group is an outer ring reaction cup. In a preferred embodiment of the application, the number of reaction cup holes per set is 4. In other embodiments of the present application, the number of reaction cup holes in each set is variable and may be 2, 3,5, 6, 8, 10, etc.

In one embodiment, the cuvette in the first set of cuvette holes is used for mixing a large amount of sample and reagent, and after mixing, a micro-amount of the mixture is sucked into the cuvette in the second set of cuvette holes by the reagent needle. For example, 10. Mu.L of sample and 190. Mu.L of reagent are first aspirated into the first set of reaction cups by the reagent needle, respectively, and after thorough mixing, 20. Mu.L of the mixture is again aspirated into the second set of reaction cups by the reagent needle.

Further, the mixing disc module 3 comprises a mixing disc rotating device for driving the mixing disc to rotate and a mixing device for uniformly mixing the reaction cups. The mixing disc rotating device comprises a mixing disc rotating motor 303 and a third driving device.

As shown in fig. 6, the mixing disc rotating motor is mounted on the fourth mounting plate 304 through a motor support 305, the section of the motor support is in a groove shape, the mixing disc rotating motor 303 is located on a back plate of the motor support far away from the fourth mounting plate, and an output shaft of the mixing disc rotating motor extends to the direction of the fourth mounting plate. The third driving device includes a third driving pulley 306, a third driven pulley 307, and a third timing belt 308. To drive the mixing disk to rotate, the output shaft of the mixing disk rotation motor 303 is connected to a third driving pulley 306, the third driving pulley 306 is connected to a third driven pulley 307 via a third timing belt 308, and the third driven pulley 307 is fixedly connected to the mixing disk 302 via a support shaft 309. Specifically, one end of the support shaft 309 is fixedly connected to the third driven pulley 307, and the other end passes through the fourth mounting plate 304 upward in the Z-axis direction and is fixedly connected to the center of the mixing disk 302, thereby realizing rotation of the mixing disk. The support shaft 309 is connected to the fourth mounting plate 304 via a bearing. That is, the fourth mounting plate 304 is stationary while the support shaft 309 rotates.

The third driven pulley 307 is a rotation angle code wheel, and in an embodiment of the present application, the reaction cup holes of the mixing disc are quarter.

In addition, a placing groove 310 is installed on one side of the fourth installation 304, and a diluent is placed in the placing groove.

As shown in fig. 5, in order to achieve the full mixing of the reaction solution, a mixing device is arranged at the other side of the fourth mounting plate, and the mixing device comprises a mixing sleeve 311, a mixing sleeve up-and-down motion motor 312, a mixing sleeve rotating motor 313 and a second sliding rail 314. Specifically, be equipped with first support frame 315 on the fourth mounting panel 304, be equipped with second slide rail 314 on the first support frame 315, second slide rail 314 sets up along the Z axle direction, the lower tip of second slide rail 314 is equipped with fifth mounting panel 316, mixing cover up-and-down motion motor 312 is installed on fifth mounting panel 316, the motor shaft sets up vertically upwards, motor shaft and lead screw 317 fixed connection, be connected with slider 318 on the lead screw, slider 318 can follow second slide rail 314 up-and-down motion, one side fixed connection second support frame 319 of slider 318, mixing cover rotating electrical machines 313 are installed to one side that second support frame 319 kept away from fourth mounting panel 304, mixing cover rotating electrical machines's output shaft passes fourth mounting panel 304 and mixing cover 311 fixed connection, mixing cover rotating electrical machines 313 can drive mixing cover 311 promptly and rotate.

In order to prevent the reaction cup from running upwards when the mixing sleeve moves upwards, the fourth mounting plate 304 is further provided with a mixing sleeve limiting device, the mixing sleeve limiting device comprises an L-shaped support 320, the lower end of the L-shaped support 320 is fixedly connected with the fourth mounting plate, the upper end of the L-shaped support is fixedly connected with a reaction cup baffle 321, and the reaction cup baffle is located right above the reaction cup.

As shown in fig. 7, the mixing sleeve is provided with 3 mixing bosses 322, and in other embodiments of the present application, the number of mixing bosses 322 is not limited, and may be 1, 2, 4, 5, 6, etc. Further, in order to obtain a balanced mixing effect, the mixing bosses 322 are equally spaced, and in the preferred embodiment of the present application, the mixing bosses 322 contact the bottom and sides of the mixing sleeve 311. The contact part of the mixing boss 322 and the reaction cup is round, so that the reaction cup is prevented from being damaged when the mixing sleeve rotates. In the application, when the mixing sleeve rotating motor 313 drives the mixing sleeve 311 to rotate, the screw rod 317 also drives the mixing sleeve 311 to move upwards, and at the moment, the mixing boss in the mixing sleeve is contacted with the reaction cup, so that the effect of vibration of the reaction cup is achieved.

In a preferred embodiment of the present application, the mixing sleeve rotating motor 313 has intermittent mixing functions of three steps, fast, medium and slow, which intermittent mixing helps promote adequate contact and reaction between the sample and the reagents. During the dwell period, the sample and the reagent have more opportunities to collide, adsorb and react with each other, which helps to improve the accuracy of the detection result.

According to the sample adding device, through the uniquely designed mixing disc module 1, the reagent needle module 2 and the mechanical arm module 3, the reaction liquid is fully mixed in a short time, so that the clinical detection efficiency can be improved, and the error caused by uneven mixing can be reduced. The specific working process is as follows:

the reaction cup gripper on the mechanical arm module is used for gripping and placing the empty reaction cup on the mixing disc, the reagent needle on the reagent needle module is used for sucking the reagent in the reagent bin and adding the reagent into the reaction cup, the reagent needle can also be used for sucking and adding the diluent into the reaction cup, and the liquid suction pump is used for adding the sample into the reaction cup. The mixing sleeve up-and-down motion motor drives the mixing sleeve to prop against the reaction cup from bottom to top through the lead screw, the reaction cup is stopped after a certain distance of upward motion due to the effect of the mixing sleeve limiting device, the mixing sleeve rotating motor rotates to drive the reaction cup to vibrate, the reaction cup position on the mixing disc is divided into 4 equal parts, the vibration of the reaction cup is accurately controlled through the rotating angle code disc, and each reaction cup rotates for 90 degrees after being uniformly mixed to carry out the mixing of the next reaction cup. After the mixing is completed, the reagent needle after cleaning transfers the evenly mixed reaction liquid suction part in the outer ring reaction cup into the inner ring reaction cup, and then the inner ring reaction cup is transferred to the incubation module for incubation through the transfer of the reaction cup.

Test example 1

In order to detect the effect of the sample adding device, 200 mu L of reaction liquid containing microspheres is added into the outer ring reaction cup, after the reaction liquid is mixed according to a normal procedure, 5 parts of reaction liquid of 20 mu L is taken out, and the number of the microspheres is checked by a scanning electron microscope, and the result shows that the number of the microspheres in different reaction liquids is not greatly different and has no obvious difference, so that the sample adding device has good mixing effect.

TABLE 1 number of microspheres in different reaction solutions in test examples

Reaction liquid	Microsphere count
		1	35
2	34
		3	36
4	35
		5	33

Comparative example 1

Based on the sample adding device, the mixing sleeve is replaced by a mixing sleeve without a boss, the detection method is the same as that of the test example, and the results are shown in table 2, and the difference of the numbers of microspheres in different reaction solutions is large.

TABLE 2 number of microspheres in different reaction solutions in comparative example

Reaction liquid	Microsphere count
		1	25
2	35
		3	20
4	45
		5	65

Comparative example 2

On the basis of the sample adding device, 20 mu L of reaction liquid containing microspheres is added into each of the four outer ring reaction cups. After mixing according to the normal procedure, the reaction solution was taken out, and the number of microspheres was checked by a scanning electron microscope, and the results are shown in table 3, wherein the number of microspheres in different reaction solutions is different, and some microspheres are lost and may remain in the reaction cup.

TABLE 3 number of microspheres in different reaction solutions in comparative example

Reaction liquid	Microsphere count
		1	25
2	20
		3	15
4	18

The above embodiments are merely illustrative of the principles of the present application and its efficacy, not in limitation of the application. The application can be modified by the concept without any material modification, which is a behavior that violates the protection scope of the application.

Claims

1. A sample adding device is characterized by comprising

The mechanical arm module is used for placing the reaction cup in the mixing disc, sucking a sample from the sample rack and adding the sample into the reaction cup;

A reagent needle module for drawing reagent from a reagent cartridge and adding into the reaction cup;

And the mixing disc module is used for loading a solution formed by the sample and the reagent.

2. The sample application device according to claim 1, wherein the robotic arm module comprises a cuvette holder for placing a cuvette in a cuvette well of a mixing tray.

3. The sample application device according to claim 1, wherein the reagent needle module comprises a reagent needle, a needle holder, and a reagent needle driving means.

4. A sample application device according to claim 3, wherein the reagent needle driving means comprises a rotary motion motor for driving the reagent needle to move in the circumferential direction, and an up-and-down motion motor for driving the reagent needle to move up and down.

5. The sample application device according to claim 1, wherein the mixing disk module comprises a mixing disk and reaction cup holes arranged on the mixing disk.

6. The sample adding device according to claim 1, wherein the mixing disc module comprises a mixing disc rotating device for driving the mixing disc to rotate and a mixing device for mixing the reaction cups.

7. The sample application device according to claim 6, wherein the mixing disc rotation device comprises a mixing disc rotation motor and a rotation angle code disc.

8. The sample adding device according to claim 6, wherein the mixing device comprises a mixing sleeve, a mixing sleeve up-and-down motor, a screw rod, a mixing sleeve limiting device and a mixing sleeve rotating motor.

9. An in vitro diagnostic device, comprising: and a sample adding device.

10. A full-automatic flow fluorescent immunoassay instrument, comprising: reagent preservation device, sample adding device, incubation device, optical detection system, software system.