CN117929705A

CN117929705A - Immunoassay device and sample automatic dilution method

Info

Publication number: CN117929705A
Application number: CN202311857335.0A
Authority: CN
Inventors: 李子超; 张栋; 何飞
Original assignee: Shenzhen Dake Medical Technology Co ltd
Current assignee: Shenzhen Dake Medical Technology Co ltd
Priority date: 2023-12-29
Filing date: 2023-12-29
Publication date: 2024-04-26

Abstract

The application relates to an immunoassay device and an automatic sample dilution method. The magnetic collection device comprises a bearing piece, wherein a sample adding position is constructed on the bearing piece and used for bearing the reaction cup; the gripper device is used for gripping and moving the reaction cup to the sample adding position; the sampling needle device can be close to or far away from the magnetic collection device, and preset reaction liquid is added into the reaction cup on the sample adding position. The immunity analysis device directly constructs a sample adding position on the bearing piece to bear the reaction cup, and uses the reaction cup at the sample adding position as a container for sample dilution, and simultaneously uses the gripper device to move the reaction cup and the sampling needle device to add and dilute samples. Therefore, the whole immunoassay device does not need to additionally design a special sample dilution device, the whole device has simple structure, small occupied space, low manufacturing cost, higher sample dilution speed and higher sample test efficiency.

Description

Immunoassay device and sample automatic dilution method

Technical Field

The application relates to the technical field of medical detection equipment, in particular to an immunoassay device and an automatic sample dilution method.

Background

The immunoassay technology has been applied more and more in recent years due to the advantages of high sensitivity, good specificity, wide linear range, high throughput, strong platform expansibility, abundant menu of measurable items, etc. In the detection process of an immunoassay device, it is necessary to dilute a sample reagent. However, in the related art, a sample diluting device is mostly specially designed in the immunoassay device, so that the whole immunoassay device has a complex structure, large occupied space and high manufacturing cost; or by manually diluting the sample, however, such dilution reduces the efficiency of the test.

Disclosure of Invention

Based on the above, it is necessary to provide an immunoassay device for solving the problems of the conventional immunoassay device, such as complicated structure, large space occupation, high cost and low test efficiency.

An immunoassay device, comprising:

The magnetic collection device comprises a bearing piece, wherein a sample adding position is constructed on the bearing piece and used for bearing the reaction cup;

the gripper device is used for gripping and moving the reaction cup to the sample adding position;

The sampling needle device can be close to or far away from the magnetic collection device, and preset reaction liquid is added into the reaction cup on the sample adding position.

In one embodiment, the sample loading positions are multiple, and the multiple sample loading positions are arranged at intervals.

In one embodiment, the immunoassay device further comprises a reagent sample loading device comprising a plurality of kits and a plurality of sample tubes;

the plurality of the reagent boxes are used for bearing different preset reagents, and the plurality of the sample tubes are used for bearing different samples.

In one embodiment, the immunoassay device further comprises a mixing apparatus;

The mixing device is provided with a mixing position, and the mixing position is used for bearing the reaction cup; the mixing equipment is used for carrying out mixing operation on the preset reaction liquid in the reaction cup;

the gripper device is also used for gripping and moving the reaction cup added with the preset reaction liquid into the mixing position.

The application also provides an automatic sample dilution method based on the immunoassay device according to any one of the above embodiments, comprising:

Controlling the magnetic collection device to move along a first direction so as to enable a first sample adding position in the sample adding positions to move to a first preset position;

grabbing and moving the first reaction cup into the first sample adding position through the grabbing device;

and controlling the sampling needle device to move, and adding a first preset reaction liquid into the first reaction cup.

In one embodiment, the step of controlling the movement of the sampling needle device and adding the first preset reaction liquid into the first reaction cup specifically includes:

controlling the sampling needle device to move to a second preset position relative to the reagent sample loading device;

Controlling a sampling needle of the sampling needle device to suck a first preset reaction liquid at the reagent sample loading device;

controlling the sampling needle device to move to a third preset position relative to the magnetic collection device;

And controlling the sampling needle to discharge the sucked first preset reaction liquid into the first reaction cup.

In one embodiment, after the step of controlling the sampling needle to discharge the sucked first preset reaction liquid into the first reaction cup, the method further comprises:

And controlling the sampling needle equipment to move to the cleaning pool and cleaning the sampling needle.

controlling the magnetic collection device to move to the first preset position along the first direction.

In one embodiment, the step of controlling the movement of the sampling needle device and adding the first preset reaction liquid into the first reaction cup further comprises:

and controlling the magnetic collection device to move to a fourth preset position along the first direction.

In one embodiment, after the step of controlling the movement of the sampling needle device and adding the first preset reaction solution into the first reaction cup, the method further includes:

The first reaction cup is grabbed and moved to a mixing position of mixing equipment through the grabbing device;

And uniformly mixing the first preset reaction liquid in the first reaction cup through the uniformly mixing equipment.

In one embodiment, the step of uniformly mixing the first preset reaction solution in the first reaction cup by the uniformly mixing device specifically includes:

And controlling the mixing equipment to eccentrically vibrate so as to enable the first reaction cup to shake.

In one embodiment, after the step of uniformly mixing the first preset reaction solution in the first reaction cup by the uniformly mixing device, the method further includes:

the first reaction cup is grabbed and moved into a second sample adding position in the sample adding positions through the grabbing device;

The second reaction cup is grabbed and moved into the first sample adding position through the grabbing device;

And controlling the sampling needle device to move, and adding a second preset reaction liquid into the second reaction cup.

In one embodiment, the step of controlling the movement of the sampling needle device and adding the second preset reaction liquid into the second reaction cup specifically includes:

controlling a sampling needle of the sampling needle device to inhale a second preset reagent in a reagent kit of the reagent sample loading device;

Controlling the sampling needle to suck the first preset reaction liquid in the first reaction cup;

And discharging the second preset reagent and the first preset reaction liquid sucked by the sampling needle into the second reaction cup.

In one embodiment, the step of discharging the second preset reagent and the first preset reaction liquid sucked by the sampling needle into the second reaction cup further comprises:

In one embodiment, after the step of controlling the sampling needle to suck the first preset reaction liquid in the first reaction cup, the method further includes:

Controlling the magnetic collection device to move along the first direction so as to enable the second sample adding displacement to move to the first preset position;

and grabbing and moving the first reaction cup to a waste cup channel through the grabbing device.

In one embodiment, after the step of controlling the movement of the sampling needle device and adding the second preset reaction solution into the second reaction cup, the method further includes:

grabbing and moving the second reaction cup to the mixing position of the mixing device through the grabbing device;

And uniformly mixing the second preset reaction liquid in the second reaction cup through the uniformly mixing equipment.

In one embodiment, before the step of moving the second reaction cup to the mixing position of the mixing device, the method further includes:

And controlling the magnetic collection device to move along the first direction so as to enable the first sample adding displacement to move to the first preset position.

In one embodiment, the step of uniformly mixing the second preset reaction solution in the second reaction cup by the uniformly mixing device specifically includes:

And controlling the mixing equipment to eccentrically vibrate so as to enable the second reaction cup to shake.

When the immunoassay device is used for carrying out sample dilution operation, the reaction cup is firstly grabbed and moved to a sample adding position on a bearing piece of the magnetic collection device through the grabbing device; then, a preset reaction liquid is added into a reaction cup on the sample adding position through sampling needle equipment, and further sample adding dilution of a sample is completed in the reaction cup. The immunoassay device directly constructs a sample adding position on a bearing piece on the magnetic collection device to bear the reaction cup, the reaction cup at the sample adding position is used as a container for sample dilution, and meanwhile, the gripper device is utilized to move the reaction cup, and the sampling needle device is used for adding and diluting the reaction cup. Therefore, the whole immunity analysis device does not need to additionally design a special sample dilution device, so the structure of the whole immunity analysis device is simpler, the occupied space is smaller, and the manufacturing cost is lower; simultaneously, compared with a manual dilution scheme, the sample dilution speed is higher, and the sample test efficiency is higher.

Drawings

Fig. 1 is a first schematic diagram of an immunoassay device according to some embodiments of the present application.

Fig. 2 is a second schematic view of the immunoassay device shown in fig. 1.

Fig. 3 is a plan view of the immunoassay device shown in fig. 1.

Fig. 4 is a schematic view of a magnetic collection device in the immunoassay device shown in fig. 1.

Fig. 5 is a schematic view of a sampling needle device in the immunoassay device shown in fig. 1.

Fig. 6 is a flow chart of a method for automatically diluting a sample according to some embodiments of the present application.

Reference numerals: 100-magnetic collection device; 110-a carrier; 111-sample adding position; 1111—a first sample addition station; 1112-a second sample addition station; 112-magnetic collection site; 120-magnet; 200-sampling needle device; 210-sampling needle; 300-uniformly mixing equipment; 310-mixing; 400-gripper device; 500-reagent sample loading apparatus; 510-a kit; 520-sample tube; 600-reaction cup loading device; 700-waste cup channel; 800-reaction cup;

Detailed Description

In order that the above objects, features and advantages of the application will be readily understood, a more particular description of the application will be rendered by reference to the appended drawings. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present application. The present application may be embodied in many other forms than described herein and similarly modified by those skilled in the art without departing from the spirit of the application, whereby the application is not limited to the specific embodiments disclosed below.

In the description of the present application, it should be understood that, if any, these terms "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc., are used herein with respect to the orientation or positional relationship shown in the drawings, these terms refer to the orientation or positional relationship for convenience of description and simplicity of description only, and do not indicate or imply that the apparatus or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore should not be construed as limiting the application.

Furthermore, the terms "first," "second," and the like, if any, are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include at least one such feature. In the description of the present application, the terms "plurality" and "a plurality" if any, mean at least two, such as two, three, etc., unless specifically defined otherwise.

In the present application, unless explicitly stated and limited otherwise, the terms "mounted," "connected," "secured," and the like are to be construed broadly. For example, the two parts can be fixedly connected, detachably connected or integrated; can be mechanically or electrically connected; either directly or indirectly, through intermediaries, or both, may be in communication with each other or in interaction with each other, unless expressly defined otherwise. The specific meaning of the above terms in the present application can be understood by those of ordinary skill in the art according to the specific circumstances.

In the present application, unless expressly stated or limited otherwise, the meaning of a first feature being "on" or "off" a second feature, and the like, is that the first and second features are either in direct contact or in indirect contact through an intervening medium. Moreover, a first feature being "above," "over" and "on" a second feature may be a first feature being directly above or obliquely above the second feature, or simply indicating that the first feature is level higher than the second feature. The first feature being "under", "below" and "beneath" the second feature may be the first feature being directly under or obliquely below the second feature, or simply indicating that the first feature is less level than the second feature.

It will be understood that if an element is referred to as being "fixed" or "disposed" on another element, it can be directly on the other element or intervening elements may also be present. If an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "upper," "lower," "left," "right," and the like as used herein, if any, are for descriptive purposes only and do not represent a unique embodiment.

The immunoassay technology has been applied more and more in recent years due to the advantages of high sensitivity, good specificity, wide linear range, high throughput, strong platform expansibility, abundant menu of measurable items, etc. In the detection process of an immunoassay device, it is necessary to dilute a sample reagent. However, in the related art, a sample diluting device is mostly specially designed in the immunoassay device, so that the whole immunoassay device has a complex structure, large occupied space and high manufacturing cost; or by manually diluting the sample, however, such dilution reduces the efficiency of the test. Based on the above problems, the present application provides an immunoassay device.

Referring to fig. 1-5, fig. 1 shows a first schematic diagram of an immunoassay device according to some embodiments of the present application. Fig. 2 shows a second schematic view of the immunoassay device shown in fig. 1. Fig. 3 shows a top view of the immunoassay device shown in fig. 1. Fig. 4 shows a schematic diagram of the magnetic collection device 100 in the immunoassay apparatus shown in fig. 1. Fig. 5 shows a schematic view of a sampling needle device 200 in the immunoassay device shown in fig. 1. An immunoassay device according to an embodiment of the present application includes a magnetic collection apparatus 100, a gripper apparatus 400, and a sampling needle apparatus 200. The magnetic collection device 100 comprises a carrier 110, wherein a sample adding position 111 is configured on the carrier 110, and the sample adding position 111 is used for carrying the reaction cup 800; the gripper device 400 is used for gripping and moving the reaction cup 800 onto the sample loading position 111; the sampling needle device 200 can be moved closer to or farther from the magnetic collection device 100 and can add a predetermined reaction solution to the reaction cup 800 at the sample addition site 111.

When the sample diluting operation is performed by the immunoassay device, the cuvette 800 is first grabbed and moved onto the sample loading position 111 on the carrier 110 of the magnetic collection device 100 by the gripper device 400; then, a preset reaction liquid is added into the reaction cup 800 on the sample adding position 111 through the sampling needle device 200, and further, the dilution of the sample is completed in the reaction cup 800. Since the immunoassay device directly constructs the sample loading position 111 on the carrier 110 on the magnetic collection device 100 to load the reaction cup 800, and uses the reaction cup 800 at the sample loading position 111 as a sample dilution container, and simultaneously uses the gripper device 400 to move the reaction cup 800 and the sampling needle device 200 to load and dilute the reaction cup 800. Therefore, the whole immunity analysis device does not need to additionally design a special sample dilution device, so the structure of the whole immunity analysis device is simpler, the occupied space is smaller, and the manufacturing cost is lower; simultaneously, compared with a manual dilution scheme, the sample dilution speed is higher, and the sample test efficiency is higher.

It should be noted that, the type of the preset reaction solution added in the reaction cup 800 at the sample adding position 111 is not limited, and may be a sample and/or a preset reagent, or may be a diluent. The sampling needle device 200 is not particularly limited, and various preset reaction liquids are added to the reaction cup 800 positioned in the sample adding position 111 for a plurality of times, thereby realizing sample adding and dilution operations in the reaction cup 800.

Referring to fig. 4, in some embodiments, the carrier 110 of the magnetic collecting device 100 is further configured with a plurality of magnetic collecting sites 112, and the periphery of the plurality of magnetic collecting sites 112 is further provided with magnets 120. The magnetic collection site 112 is used to carry a reaction cup 800 containing magnetic beads and magnetic bead complexes. After the incubation reaction of the reagent in the reaction cup 800 containing the magnetic beads and the magnetic bead complexes is completed, the reaction cup 800 is grabbed and moved to the magnetic collection position 112, the magnetic beads and the magnetic bead complexes are collected on the cup wall of the reaction cup 800 under the action of the magnetic field of the magnet 120, and the part of the reaction object, which is not combined with the magnetic beads, is removed. The above description is a conventional structure and function of the magnetic collection device 100, but in the present application, the conventional structure of the magnetic collection device 100 is improved, so that the sample loading position 111 is added on the carrier 110 of the magnetic collection device 100, and thus the sample diluting and loading operation is completed at the position of the magnetic collection device 100, so that the whole structural layout of the immunoassay device is more compact, the volume is smaller, and the manufacturing cost is lower.

Referring to fig. 4, in some embodiments, the loading bits 111 have a plurality of loading bits 111, and the loading bits 111 are spaced apart. Through setting up a plurality of application of sample positions 111 to make can place different reaction cups 800 respectively on a plurality of application of sample positions 111, and then make the sample dilution in-process, can carry out a plurality of sample dilution thread operations simultaneously, further improved the efficiency of sample dilution. Referring to fig. 4, in one embodiment, the sample loading sites 111 have two. The two loading sites 111 are spaced apart along the first direction.

Referring to fig. 1-3, in some embodiments, the immunoassay device further comprises a reagent sample loading device 500, the reagent sample loading device 500 comprising a plurality of reagent cartridges 510 and a sample tube 520; the plurality of kits 510 are used to carry different preset reagents. A plurality of sample tubes 520 are used to carry different samples. Through carrying different preset reagents in the plurality of reagent boxes 510, different detection samples are carried in the plurality of sample tubes 520, so that the immunoassay device can adapt to different detection requirements.

Referring to fig. 3, in some embodiments, the immunoassay device further includes a mixing apparatus 300, wherein the mixing apparatus 300 is configured with a mixing position 310, and the mixing position 310 is used to carry a reaction cup 800; the mixing device 300 is used for uniformly mixing the preset reaction liquid in the reaction cup 800; the gripper apparatus 400 is further configured to grip and move the cuvette 800 filled with the preset reaction solution into the mixing section 310.

Referring to fig. 3, in some embodiments, the immunoassay device further includes a cuvette loading unit 600, and the cuvette loading unit 600 is configured to load and cache a new cuvette 800.

Referring to fig. 3, in some embodiments, the immunoassay device further includes a waste cup channel 700, wherein the waste cup channel 700 is used to buffer the used waste reaction cup 800, thereby facilitating subsequent collection and disposal of the waste reaction cup 800.

The present application also provides an automatic sample dilution method, referring to fig. 6, fig. 6 is a flowchart of an automatic sample dilution method according to some embodiments of the present application, which is based on the immunoassay device according to any one of the embodiments, and the automatic sample dilution method includes:

S10: controlling the magnetic collection device 100 to move along a first direction, specifically, a first direction xx' in fig. 2 and 3, so as to move a first sample adding position 1111 of the sample adding positions 111 to a first preset position;

S20: the first reaction cup is grabbed and moved into the first sample adding position 1111 through the grabbing device 400;

s30: the sampling needle device 200 is controlled to move, and a first preset reaction liquid is added into the first reaction cup.

When the sample needs to be diluted, the magnetic collection device 100 is controlled to move along the first direction, so that the first sample adding position 1111 moves to a first preset position, that is, a preset position where the cuvette 800 is shifted. The gripper apparatus 400 then grips and moves the first cuvette from the cuvette loading unit 600 of the immunoassay device onto the first loading site 1111. The sampling needle device 200 adds a first preset reaction liquid into the first reaction cup at the first sample adding position 1111, so as to complete sample adding dilution in the reaction cup 800. Since the sample automatic dilution method provided by the application is to load the first reaction cup through the first sample loading position 1111 constructed on the load member 110 on the magnetic collection device 100, and take the first reaction cup as a sample dilution container, and simultaneously, the gripper device 400 is utilized to move the first reaction cup, and the sampling needle device 200 loads and dilutes the first reaction cup. Therefore, the whole sample adding and diluting process is realized without additionally designing a special sample diluting device, so that the method is simple and convenient; simultaneously, compared with a manual dilution scheme, the sample dilution speed is higher, and the sample test efficiency is higher.

It should be noted that, the type of the first preset reaction solution added into the first reaction cup is not limited, and may be a sample and/or a preset reagent, or may be a diluent. The sampling needle device 200 can add different first preset reaction liquids to the first reaction cup for a plurality of times, so that the sample adding and diluting operations in the first reaction cup are realized, and the sampling needle device is not particularly limited.

In some of these embodiments, step S30 of the sample autodilution method: the specific steps of controlling the movement of the sampling needle device 200 and adding the first preset reaction liquid into the first reaction cup include:

The sample needle device 200 is controlled to move to a second preset position relative to the reagent sample loading device 500. The sample needle device 200 is controlled to move in the xx ', yy ' and zz ' directions in fig. 2 such that the sample needle device 200 moves to a second preset position relative to the reagent sample loading device 500, i.e. above the reagent cartridge 510 or sample tube.

The sampling needle 210 of the sampling needle device 200 is controlled to aspirate a first preset reaction solution at the reagent sample loading device 500. The first preset reaction liquid may be a first preset reagent sucked into the reagent cartridge 510 of the reagent sample loading apparatus 500, or may be a sample sucked into the sample tube 520 of the reagent sample loading apparatus 500; of course, a mixture of the first predetermined reagent and the sample is also possible. When the sampling needle device 200 is moved to the second preset position, the sampling needle 210 is now controlled to move up and down in the zz' direction, thereby enabling the sampling needle 210 to extend into the reagent cartridge 510 and aspirate the first preset reagent within the reagent cartridge 510 and/or to extend into the sample tube 520 and aspirate the sample within the sample tube 520.

The sampling needle device 200 is controlled to move to a third preset position relative to the magnetic collection device 100. When the sampling needle 210 sucks the first preset reaction liquid, the sampling needle device 200 is controlled to move along the xx ', yy ' and zz ' directions in fig. 2, so that the sampling needle device 200 moves to a third preset position relative to the magnetic collection device 100, so that the subsequent sampling needle 210 can discharge the first preset reaction liquid into the first reaction cup conveniently.

The sampling needle 210 is controlled to discharge the sucked first preset reaction liquid into the first reaction cup. When the sampling needle device 200 moves to the third preset position with respect to the magnetic collection device 100, the sampling needle 210 is controlled to move up and down in the zz' direction in fig. 2, and the air pressure in the sampling needle 210 is controlled so that the sampling needle 210 can discharge the sucked first preset reaction liquid into the first reaction cup.

In some embodiments, after the step of controlling the sampling needle 210 to discharge the sucked first preset reaction liquid into the first reaction cup, the method further comprises: the sampling needle device 200 is controlled to move to a washing bath (not shown) and a washing operation is performed on the sampling needle 210. After the first preset reaction solution is discharged into the first reaction cup, the sampling needle device 200 is controlled to move along xx ' and yy ' directions in fig. 2 and 3, so that the sampling needle device 200 moves to the cleaning pool, and the sampling needle 210 moves to the cleaning pool by controlling the sampling needle 210 to move along zz ' direction in fig. 2, and the inner wall and the outer wall of the sampling needle 210 are cleaned by the combination of fluid components such as a cleaning pump, an inner wall cleaning valve, a vacuum pump and the like in the cleaning pool, so that reagent or sample residues in the sampling needle 210 are avoided, and the sampling needle 210 is convenient for performing next liquid sucking and discharging operation.

In some embodiments, after the step of controlling the sampling needle 210 to discharge the sucked first preset reaction liquid into the first reaction cup, the method further comprises: the magnetic collection device 100 is controlled to move in a first direction to a first preset position. When the sampling needle 210 discharges the first preset reaction liquid into the first reaction cup, the magnetic collection device 100 is controlled to move to the first preset position along the first direction, that is, the transfer position of the reaction cup 800. Thereby facilitating the follow-up grabbing and moving when carrying out the uniform mixing operation to the first preset reaction liquid in the first reaction cup.

In some of these embodiments, at step S30: the movement of the sampling needle device 200 is controlled, and before the first preset reaction liquid is added into the first reaction cup, the method further comprises the steps of: the magnetic collection device 100 is controlled to move in the first direction to a fourth preset position. When the first preset reaction liquid needs to be added into the first reaction cup, the magnetic collection device 100 is moved in the first direction to a fourth preset position, namely, a liquid moving position, at which the sampling needle 210 is convenient to move up and down along the zz' direction in fig. 2, so that the first reaction cup is convenient to be subjected to sample adding and dilution operation.

In some of these embodiments, at step S30: the step of controlling the movement of the sampling needle device 200 and adding the first preset reaction liquid into the first reaction cup further comprises the following steps:

S40: the first reaction cup is grabbed and moved to a mixing position 310 of the mixing device 300 by a grabbing device 400; s50: the first preset reaction liquid in the first reaction cup is uniformly mixed by the uniformly mixing device 300. After the first preset reaction liquid is added into the first reaction cup, the gripper device 400 grabs the first reaction cup from the first sample adding position 1111 and moves the first reaction cup into the mixing position 310 of the mixing device, and then the mixing device 300 is used for uniformly mixing the first preset reaction liquid in the first reaction cup, so that the sample adding dilution and uniform mixing operation in the first reaction cup is completed, the first preset reaction liquid in the first reaction cup is more uniform, and the final analysis and detection result is more accurate.

In some of these embodiments, step S50: the mixing operation for the first preset reaction liquid in the first reaction cup by the mixing device 300 specifically includes: the mixing apparatus 300 is controlled to oscillate eccentrically to shake the first reaction cup. Through the eccentric vibration of the mixing device 300, the first reaction cup is further enabled to shake, and therefore the first preset reaction liquid in the first reaction cup is evenly mixed in the shaking process.

In some of these embodiments, step S50: after the mixing device 300 performs the mixing operation on the first preset reaction solution in the first reaction cup, the method further includes: step S60: the first reaction cup is grabbed and moved into a second sample adding position 1112 in the sample adding positions 111 through the grabbing device 400; step S70: the second reaction cup is grabbed and moved into the first sample adding position 1111 through the grabbing device 400; step S80: the sampling needle device 200 is controlled to move, and a second preset reaction liquid is added into the second reaction cup.

After the first preset reaction solution is uniformly mixed, the first reaction cup is grabbed and moved into the second sample adding position 1112 through the gripper device 400 so as to make the first sample adding position 1111 free; then the gripper apparatus 400 grips and moves a new cuvette 800, i.e. a second cuvette, into the first loading site 1111; and further controls the movement of the sampling needle device 200, and adds a second preset reaction liquid into the second reaction cup, thereby realizing the sample adding or dilution operation in the second reaction cup.

In some of these embodiments, step S80: the movement of the sampling needle device 200 is controlled, and the adding of the second preset reaction liquid into the second reaction cup specifically comprises: controlling the sampling needle device 200 to move to a second preset position relative to the reagent sample loading device 500; controlling the sampling needle 210 of the sampling needle device 200 to inhale a second preset reagent within the reagent cartridge 510 of the reagent sample loading device 500; controlling the sampling needle device 200 to move to a third preset position relative to the magnetic collection device 100; controlling the sampling needle 210 to suck a first preset reaction liquid in the first reaction cup; the second preset reagent and the first preset reaction liquid sucked in by the sampling needle 210 are discharged into the second reaction cup.

When a second preset reaction solution needs to be added into the second reaction cup, the sampling needle device 200 is controlled to move along the xx ' and yy ' directions in fig. 2 and 3, so that the sampling needle device 200 moves to a second preset position relative to the reagent sample loading device 500, and then the sampling needle 210 is controlled to move up and down along the zz ' direction in fig. 2, so that the sampling needle 210 sucks a second preset reagent in the reagent box 510; then, the sampling needle device 200 is controlled to move along the xx ' and yy ' directions in fig. 2 and 3 so as to move to a third preset position relative to the magnetic collection device 100, and then the sampling needle 210 is controlled to move up and down along the zz ' direction in fig. 2 so that the sampling needle 210 sucks the first preset reaction liquid in the first reaction cup; and finally, the second preset reagent and the first preset reaction liquid sucked by the sampling needle 210 are all discharged into the second reaction cup, so that the mixed liquid of the second preset reagent and the first preset reaction liquid is carried in the second reaction cup. The second preset reaction liquid is a mixed liquid of the second preset reagent and the first preset reaction liquid.

In some embodiments, the step of adding the second preset reagent and the first preset reaction liquid inhaled by the sampling needle 210 into the second reaction cup further comprises: the sampling needle device 200 is controlled to move to the washing bath and the sampling needle 210 is subjected to the washing operation. After the sampling needle 210 adds the inhaled second preset reagent and the first preset reaction liquid into the second reaction cup, the sampling needle device 200 is controlled to move along xx ' and yy ' directions in fig. 2 and 3, so that the sampling needle device 200 moves to the cleaning pool, and the sampling needle 210 moves into the cleaning pool by controlling the sampling needle 210 to move up and down along zz ' direction in fig. 2, and the inner wall and the outer wall of the sampling needle 210 are cleaned by the combination of fluid components such as a cleaning pump, an inner wall cleaning valve, a vacuum pump and the like in the cleaning pool, so that reagent or sample residues in the sampling needle 210 are avoided, and the sampling needle 210 is convenient to perform next liquid sucking and draining operation.

In some embodiments, after the step of controlling the sampling needle 210 to suck the first preset reaction liquid in the first reaction cup, the method further comprises: controlling the magnetic collection device 100 to move in a first direction so that the second sample application position 1112 moves to a first predetermined position; the first cuvette is grabbed and moved to the cuvette channel 700 by the grabber 400. After the sampling needle 210 sucks the first preset reaction liquid in the first reaction cup, the magnetic collection device 100 is controlled to move along the first direction, that is, xx' in fig. 2 and 3, so that the second sample loading position 1112 carrying the first reaction cup moves to the first preset position, that is, the transfer position of the reaction cup 800, thereby facilitating the gripper device 400 to grasp and move the first reaction cup to the waste cup channel 700, and preventing motion interference phenomenon.

In some of these embodiments, at step S80: after controlling the movement of the sampling needle device 200 and adding the second preset reaction liquid into the second reaction cup, the method further comprises: s90: grabbing and moving the second reaction cup to a mixing position 310 of the mixing device 300 through a grabbing device 400; s100: and uniformly mixing the second preset reaction liquid in the second reaction cup through the uniformly mixing equipment 300. After the second preset reaction liquid is added into the second reaction cup, the second reaction cup is grabbed and moved to the mixing position 310 of the mixing device 300 through the gripper device 400, so that the second preset reaction liquid is uniformly mixed through the mixing device 300, the sample addition dilution and uniform mixing operation in the second reaction cup is completed, the second preset reaction liquid in the second reaction cup is more uniform, and the final analysis and detection result is more accurate. Since the second preset reaction solution is a mixed reagent of the first preset reaction solution and the third preset reaction solution, the mixed reagent of the first preset reaction solution and the third mixed reagent can be mixed more uniformly after being uniformly mixed by the uniformly mixing device 300.

In some of these embodiments, at step S90: before the step of grabbing and moving the second reaction cup to the mixing position 310 of the mixing device 300 by the grabbing device 400, the method further comprises: the magnetic collection device 100 is controlled to move in a first direction to move the first loading bit 1111 to a first preset position. Before the second reaction cup needs to be grabbed and moved to the mixing position 310 of the mixing device 300, the magnetic collection device 100 is controlled to move along the first direction, so that the first sample adding position 1111 carrying the second reaction cup is located at the first preset position, namely the shifting position of the reaction cup 800, and then the grabbing movement is performed through the gripper device 400, so that the grabbing movement process of the gripper device 400 is relatively convenient, and the motion interference phenomenon is not easy to occur.

In some of these embodiments, step S100: the step of uniformly mixing the second preset reaction solution in the second reaction cup by the uniformly mixing device 300 specifically includes: the mixing apparatus 300 is controlled to oscillate eccentrically to shake the second reaction cup. Through the eccentric vibration of the mixing device 300, the second reaction cup is further enabled to shake, and therefore second preset reaction liquid in the second reaction cup is evenly mixed in the shaking process.

It should be understood that, in the embodiment of the present application, at least a part of the steps in the preparation method may include a plurality of steps or a plurality of stages, which are not necessarily performed at the same time, but may be performed at different times, and the order of performing the steps or stages is not necessarily performed sequentially, but may be performed alternately or alternately with other steps or at least a part of the steps or stages in other steps.

The technical features of the above-described embodiments may be arbitrarily combined, and all possible combinations of the technical features in the above-described embodiments are not described for brevity of description, however, as long as there is no contradiction between the combinations of the technical features, they should be considered as the scope of the description.

The above examples illustrate only a few embodiments of the application, which are described in detail and are not to be construed as limiting the scope of the claims. It should be noted that it will be apparent to those skilled in the art that several variations and modifications can be made without departing from the spirit of the application, which are all within the scope of the application. Accordingly, the scope of protection of the present application is to be determined by the appended claims.

Claims

1. An immunoassay device, comprising:

2. The immunoassay device of claim 1, wherein said loading sites are provided in a plurality of spaced apart positions.

3. The immunoassay device of claim 1, further comprising a reagent sample loading apparatus comprising a plurality of kits and a plurality of sample tubes;

The plurality of the kits are used for bearing different preset reagents; a plurality of the sample tubes are used to carry different samples.

4. The immunoassay device of any of claims 1-3, wherein the immunoassay device further comprises a mixing apparatus;

5. A method of automatic sample dilution, characterized in that it comprises, based on the immunoassay device of any one of claims 1 to 4:

6. The method of automatic sample dilution according to claim 5, wherein the step of controlling the movement of the sampling needle device and adding a first predetermined reaction liquid into the first reaction cup comprises:

7. The method according to claim 6, further comprising, after the step of controlling the sampling needle to discharge the sucked first preset reaction liquid into the first cuvette:

8. The method according to claim 6, further comprising, after the step of controlling the sampling needle to discharge the sucked first preset reaction liquid into the first cuvette:

9. The method of automatic sample dilution according to claim 5, further comprising, prior to the step of controlling movement of the sampling needle device and adding a first predetermined reaction liquid to the first cuvette:

10. The method of automatic sample dilution according to any one of claims 5-9, further comprising, after the step of controlling movement of the sampling needle device and adding a first predetermined reaction liquid to the first cuvette:

11. The method for automatically diluting a sample according to claim 10, wherein the step of uniformly mixing the first preset reaction solution in the first reaction cup by the uniform mixing device comprises:

12. The method according to claim 10, further comprising, after the step of mixing the first preset reaction solution in the first reaction cup by the mixing device:

13. The method of automatic sample dilution according to claim 12, wherein the step of controlling the movement of the sampling needle device and adding a second predetermined reaction solution to the second reaction cup comprises:

14. The method of automatic sample dilution according to claim 13, further comprising, after the step of discharging the second preset reagent and the first preset reaction liquid sucked by the sampling needle into the second reaction cup:

15. The method of automatic sample dilution according to claim 13, further comprising, after the step of controlling the sampling needle to aspirate the first preset reaction liquid in the first cuvette:

16. The method of automatic sample dilution according to claim 12, further comprising, after the step of controlling movement of the sampling needle device and adding a second predetermined reaction solution to the second reaction cup:

17. The method of automatic sample dilution according to claim 16, further comprising, prior to the step of moving the second cuvette grip to the mixing section of the mixing apparatus by the gripper apparatus:

The magnetic collection device (100) is controlled to move along the first direction so as to enable the first sample adding displacement to move to the first preset position.

18. The method for automatically diluting a sample according to claim 16, wherein the step of uniformly mixing the second preset reaction solution in the second reaction cup by the uniform mixing device comprises: