CN117925573A - Dna编辑工具、系统及其用途 - Google Patents
Dna编辑工具、系统及其用途 Download PDFInfo
- Publication number
- CN117925573A CN117925573A CN202410081243.6A CN202410081243A CN117925573A CN 117925573 A CN117925573 A CN 117925573A CN 202410081243 A CN202410081243 A CN 202410081243A CN 117925573 A CN117925573 A CN 117925573A
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- iscb
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- polynucleotide
- polypeptide
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Links
- 238000010442 DNA editing Methods 0.000 title description 2
- 229920001184 polypeptide Polymers 0.000 claims abstract description 34
- 102000004196 processed proteins & peptides Human genes 0.000 claims abstract description 34
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 34
- 108091033319 polynucleotide Proteins 0.000 claims description 39
- 102000040430 polynucleotide Human genes 0.000 claims description 39
- 239000002157 polynucleotide Substances 0.000 claims description 39
- 102000039446 nucleic acids Human genes 0.000 claims description 18
- 108020004707 nucleic acids Proteins 0.000 claims description 18
- 150000007523 nucleic acids Chemical class 0.000 claims description 18
- 239000013598 vector Substances 0.000 claims description 16
- 239000000203 mixture Substances 0.000 claims description 11
- 125000003275 alpha amino acid group Chemical group 0.000 claims description 7
- 238000002360 preparation method Methods 0.000 claims description 3
- 239000003153 chemical reaction reagent Substances 0.000 claims 1
- 230000007018 DNA scission Effects 0.000 abstract description 3
- 108020004414 DNA Proteins 0.000 description 33
- 238000003776 cleavage reaction Methods 0.000 description 16
- 230000007017 scission Effects 0.000 description 16
- 239000013612 plasmid Substances 0.000 description 14
- 241000588724 Escherichia coli Species 0.000 description 13
- 108010042407 Endonucleases Proteins 0.000 description 10
- 102000004533 Endonucleases Human genes 0.000 description 10
- 108020005004 Guide RNA Proteins 0.000 description 10
- 230000000694 effects Effects 0.000 description 10
- 108090000623 proteins and genes Proteins 0.000 description 7
- 238000013518 transcription Methods 0.000 description 7
- 230000035897 transcription Effects 0.000 description 7
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- 229960005091 chloramphenicol Drugs 0.000 description 6
- 239000013613 expression plasmid Substances 0.000 description 6
- 239000002773 nucleotide Substances 0.000 description 6
- 125000003729 nucleotide group Chemical group 0.000 description 6
- 102000004169 proteins and genes Human genes 0.000 description 6
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 5
- 108091026890 Coding region Proteins 0.000 description 4
- 238000012986 modification Methods 0.000 description 4
- 230000004048 modification Effects 0.000 description 4
- 239000013607 AAV vector Substances 0.000 description 3
- 238000003780 insertion Methods 0.000 description 3
- 230000037431 insertion Effects 0.000 description 3
- 108091033409 CRISPR Proteins 0.000 description 2
- 102000053602 DNA Human genes 0.000 description 2
- 108010008532 Deoxyribonuclease I Proteins 0.000 description 2
- 102000007260 Deoxyribonuclease I Human genes 0.000 description 2
- 241000700584 Simplexvirus Species 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 230000000295 complement effect Effects 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 238000012217 deletion Methods 0.000 description 2
- 230000037430 deletion Effects 0.000 description 2
- 210000003527 eukaryotic cell Anatomy 0.000 description 2
- 238000010362 genome editing Methods 0.000 description 2
- 230000035772 mutation Effects 0.000 description 2
- 101150071322 ruvC gene Proteins 0.000 description 2
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 1
- 241001655883 Adeno-associated virus - 1 Species 0.000 description 1
- 241000702423 Adeno-associated virus - 2 Species 0.000 description 1
- 241000202702 Adeno-associated virus - 3 Species 0.000 description 1
- 241000580270 Adeno-associated virus - 4 Species 0.000 description 1
- 241001634120 Adeno-associated virus - 5 Species 0.000 description 1
- 241000972680 Adeno-associated virus - 6 Species 0.000 description 1
- 241001164823 Adeno-associated virus - 7 Species 0.000 description 1
- 241001164825 Adeno-associated virus - 8 Species 0.000 description 1
- 241000649045 Adeno-associated virus 10 Species 0.000 description 1
- 241000649046 Adeno-associated virus 11 Species 0.000 description 1
- 241000649047 Adeno-associated virus 12 Species 0.000 description 1
- 241000300529 Adeno-associated virus 13 Species 0.000 description 1
- 241000425548 Adeno-associated virus 3A Species 0.000 description 1
- 241000958487 Adeno-associated virus 3B Species 0.000 description 1
- 108010040467 CRISPR-Associated Proteins Proteins 0.000 description 1
- 102100024482 Cell division cycle-associated protein 4 Human genes 0.000 description 1
- 108020004705 Codon Proteins 0.000 description 1
- 241000702421 Dependoparvovirus Species 0.000 description 1
- 241000282326 Felis catus Species 0.000 description 1
- 101100383112 Homo sapiens CDCA4 gene Proteins 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000003828 downregulation Effects 0.000 description 1
- 238000004520 electroporation Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000012165 high-throughput sequencing Methods 0.000 description 1
- 230000006801 homologous recombination Effects 0.000 description 1
- 238000002744 homologous recombination Methods 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 101150109249 lacI gene Proteins 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000002715 modification method Methods 0.000 description 1
- KQDIGHIVUUADBZ-PEDHHIEDSA-N pentigetide Chemical compound OC(=O)C[C@H](N)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(O)=O)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCCNC(N)=N)C(O)=O KQDIGHIVUUADBZ-PEDHHIEDSA-N 0.000 description 1
- 230000001177 retroviral effect Effects 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 125000006850 spacer group Chemical group 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 230000003827 upregulation Effects 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
Description
Claims (8)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN2023142506 | 2023-12-25 | ||
CNPCT/CN2023/142506 | 2023-12-25 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN117925573A true CN117925573A (zh) | 2024-04-26 |
CN117925573A8 CN117925573A8 (zh) | 2024-06-25 |
Family
ID=90755089
Family Applications (6)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202410082510.1A Pending CN117925575A (zh) | 2023-12-25 | 2024-01-19 | Rna指导的dna核酸酶、载体、系统及其用途 |
CN202410081239.XA Pending CN117925572A (zh) | 2023-12-25 | 2024-01-19 | Dna编辑工具及其用途 |
CN202410081420.0A Pending CN118064404A (zh) | 2023-12-25 | 2024-01-19 | Rna指导的dna核酸酶、包含其的系统及其用途 |
CN202410081243.6A Pending CN117925573A (zh) | 2023-12-25 | 2024-01-19 | Dna编辑工具、系统及其用途 |
CN202410081381.4A Pending CN118240801A (zh) | 2023-12-25 | 2024-01-19 | IscB多肽、编码其的多核苷酸及其用途 |
CN202410082322.9A Pending CN117925574A (zh) | 2023-12-25 | 2024-01-19 | IscB多肽、包含其的载体及其用途 |
Family Applications Before (3)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202410082510.1A Pending CN117925575A (zh) | 2023-12-25 | 2024-01-19 | Rna指导的dna核酸酶、载体、系统及其用途 |
CN202410081239.XA Pending CN117925572A (zh) | 2023-12-25 | 2024-01-19 | Dna编辑工具及其用途 |
CN202410081420.0A Pending CN118064404A (zh) | 2023-12-25 | 2024-01-19 | Rna指导的dna核酸酶、包含其的系统及其用途 |
Family Applications After (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202410081381.4A Pending CN118240801A (zh) | 2023-12-25 | 2024-01-19 | IscB多肽、编码其的多核苷酸及其用途 |
CN202410082322.9A Pending CN117925574A (zh) | 2023-12-25 | 2024-01-19 | IscB多肽、包含其的载体及其用途 |
Country Status (1)
Country | Link |
---|---|
CN (6) | CN117925575A (zh) |
-
2024
- 2024-01-19 CN CN202410082510.1A patent/CN117925575A/zh active Pending
- 2024-01-19 CN CN202410081239.XA patent/CN117925572A/zh active Pending
- 2024-01-19 CN CN202410081420.0A patent/CN118064404A/zh active Pending
- 2024-01-19 CN CN202410081243.6A patent/CN117925573A/zh active Pending
- 2024-01-19 CN CN202410081381.4A patent/CN118240801A/zh active Pending
- 2024-01-19 CN CN202410082322.9A patent/CN117925574A/zh active Pending
Also Published As
Publication number | Publication date |
---|---|
CN117925575A (zh) | 2024-04-26 |
CN117925572A (zh) | 2024-04-26 |
CN118240801A (zh) | 2024-06-25 |
CN117925574A8 (zh) | 2024-06-25 |
CN118064404A (zh) | 2024-05-24 |
CN117925575A8 (zh) | 2024-06-14 |
CN117925572A8 (zh) | 2024-06-14 |
CN117925573A8 (zh) | 2024-06-25 |
CN117925574A (zh) | 2024-04-26 |
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Legal Events
Date | Code | Title | Description |
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PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
CI02 | Correction of invention patent application |
Correction item: National priority Correct: PCT/CN2023/142506 2023.12.27 CN False: PCT/CN2023/142506 2023.12.25 CN Number: 17-02 Page: The title page Volume: 40 Correction item: National priority Correct: PCT/CN2023/142506 2023.12.27 CN False: PCT/CN2023/142506 2023.12.25 CN Number: 17-02 Volume: 40 |