CN117883312A

CN117883312A - High-transdermal easy-absorption supermolecule bird's nest peptide preparation

Info

Publication number: CN117883312A
Application number: CN202410081028.6A
Authority: CN
Inventors: 范群艳; 陈奶寿; 白伟娟; 宋心宇; 郭宝忠; 银小倩; 叶淑贤
Original assignee: Xiamen Yanzhiwu Silong Biotechnology Co ltd
Current assignee: Xiamen Yanzhiwu Silong Biotechnology Co ltd
Priority date: 2024-01-19
Filing date: 2024-01-19
Publication date: 2024-04-16

Abstract

The invention discloses a supermolecule bird's nest peptide preparation with high transdermal and easy absorption. The composition comprises: 0.1 to 10 parts by weight of bird's nest peptide, 0.5 to 8 parts by weight of hydrogenated lecithin, 10 to 40 parts by weight of polydimethylsiloxane, 0.5 to 5 parts by weight of caprylic/capric triglyceride, 20 to 60 parts by weight of glycerin, 1 to 3 parts by weight of 1, 2-hexanediol, 0.1 to 5 parts by weight of phytosterol/octyldodecanol lauroyl glutamate, 0.001 to 0.5 part by weight of ceramide NP, 0.01 to 0.5 part by weight of stearic acid, 0.01 to 0.5 part by weight of palmitic acid, 0.01 to 0.5 part by weight of cholesterol, and not more than 68 parts by weight of water, wherein the hydrogenated lecithin is embedded with the bird's nest peptide. The composition can promote transdermal absorption of active substances, has good stability, and has effects of moistening, whitening, and resisting aging.

Description

High-transdermal easy-absorption supermolecule bird's nest peptide preparation

Technical Field

The invention belongs to the technical field of daily chemicals, relates to a high-transdermal easy-absorption supermolecule bird's nest peptide preparation, and particularly comprises a composition for embedding bird's nest peptide, a preparation method thereof and a skin care product.

Background

With the improvement of the living standard of people, the functional cosmetics draw great attention, and the functional cosmetics should make the functional components permeate the stratum corneum as much as possible, permanently play a role in nutrition, protection or treatment on local tissues and cells, and enter blood circulation less, so that the efficacy is improved. Finding a way to promote the percutaneous absorption of functional ingredients is one of the keys to develop and study such cosmetics.

Disclosure of Invention

The present application is mainly based on the following problems and findings:

The edible bird's nest peptide is produced by taking edible bird's nest as a raw material through a hydrolysis process, and the product with the main component having the relative molecular mass lower than 10000 has the effects of anti-inflammatory, antioxidant, whitening, anti-wrinkle, repairing and the like, and has wide application prospect in the fields of foods, biological medicines, cosmetics and the like based on the characteristics. The bird's nest peptide is used as a water-soluble functional substance, has limited transdermal absorption capacity through skin, poor thermal stability and certain characteristic smell, has low encapsulation rate on the bird's nest peptide through the existing encapsulation technology, and greatly inhibits the development of the bird's nest peptide. Therefore, how to provide a preparation method of supermolecule-coated nidus Collocaliae peptide with high encapsulation efficiency, stability, effectiveness and no obvious smell is a technical problem to be solved by the technicians in the field.

The present invention aims to solve at least one of the technical problems in the related art to some extent. Therefore, the invention aims to provide a supermolecule bird's nest peptide preparation with high transdermal and easy absorption so as to promote the transdermal absorption of bird's nest peptide and improve the stability of the product, so that the bird's nest peptide preparation has the effects of moisturizing, whitening, resisting aging and the like.

In a first aspect of the invention, the invention provides a composition for embedding bird's nest peptide. According to an embodiment of the invention, the composition comprises:

0.1 to 10 parts by weight of bird's nest peptide, 0.5 to 8 parts by weight of hydrogenated lecithin, 10 to 40 parts by weight of polydimethylsiloxane, 0.5 to 5 parts by weight of caprylic/capric triglyceride, 20 to 60 parts by weight of glycerin, 1 to 3 parts by weight of 1, 2-hexanediol, 0.1 to 5 parts by weight of phytosterol/octyldodecanol lauroyl glutamate, 0.001 to 0.5 part by weight of ceramide NP, 0.01 to 0.5 part by weight of stearic acid, 0.01 to 0.5 part by weight of palmitic acid, 0.01 to 0.5 part by weight of cholesterol, and not more than 68 parts by weight of water, wherein the hydrogenated lecithin is embedded with the bird's nest peptide.

The inventor finds that supermolecule encapsulation liposome can be used as a carrier to encapsulate the bird's nest peptide, so that the absorption of the bird's nest peptide by skin can be promoted, and the stability and the utilization rate of the bird's nest peptide in a cosmetic system can be improved. The liposome is a completely-closed multi-layer vesicle with a bilayer formed by lipoid such as phospholipid, has a structure similar to cells, can encapsulate two substances of water solubility and fat solubility, is formed by wrapping with supermolecule microcapsules, and has the characteristics of excellent transdermal absorptivity, long residence time at a skin target point and good stability. The composition for embedding the bird's nest peptide has at least the following advantages: 1. the hydrogenated lecithin is used for forming liposome, hydrophilic and lipophilic functional components can be embedded and carried at the same time, and the bird's nest peptide is wrapped in the liposome, so that the encapsulation efficiency is high, the stability of the bird's nest peptide can be obviously increased, the characteristic smell of the bird's nest peptide can be reduced, the transdermal absorption capacity of unstable active substances such as the bird's nest peptide can be promoted, the stability of the composition can be improved, and a new thought is provided for cosmetics with the effects of moisturizing, whitening, resisting aging and the like; 2. by further combining ceramide NP, polydimethylsiloxane, caprylic/capric triglyceride, glycerol, 1, 2-hexanediol, phytosterol/octyldodecanol lauroyl glutamate, stearic acid, palmitic acid, cholesterol, the composition has better moisturizing, whitening, anti-aging and other effects; 3. the composition meets the given requirements by comprehensively controlling the dosages of all the components and water, is not only beneficial to the dispersion of the bird's nest peptide and all the components and improves the stability of the composition, but also is beneficial to further improving the effects of moisturizing, whitening, resisting aging and the like of the composition.

In addition, the composition for embedding the bird's nest peptide according to the above embodiment of the present invention may have the following additional technical features:

In some embodiments of the invention, the composition of embedding the bird's nest peptide further comprises: 0 to 5 weight portions of emulsifying agent, 0 to 10 weight portions of emollient and 0 to 10 weight portions of active substance.

In some embodiments of the invention, the emulsifier comprises one or more of lecithin, hydrogenated lecithin, palmitic acid, stearic acid, olive oil PEG-7 esters, cholesterol, PEG-8 caprylic/capric glycerides, phytosterol/canola glycerides, sorbitan olive oleate, polysorbate-20, laureth-23, PEG-40 stearate, steareth-21, palmitols and mixtures of glyceryl stearate and steareth-20, oleyl erucate, polysorbate-80, mixtures of glyceryl stearate and PEG-75 stearate, C12-16 alcohol, octyldodecanol, PEG-30 dimerized hydroxystearate, cetostearyl ether-25.

In some embodiments of the present invention, the emollient comprises one or more of dimethicone, 1, 2-hexanediol, 1, 3-butanediol, 1, 2-pentanediol, caprylic/capric triglyceride, dimethiconol, isododecane, isohexadecane, squalane, halfparaffin, cetostearyl alcohol isononanoate, oleic acid capric acid, myristyl alcohol caprate, octyldodecanol, mineral oil, ethylhexyl stearate, coco-caprylate/caprate, shea butter, rapeseed oil, polydecene, shea butter, soybean oil, olive oil, palmitic acid, stearic acid, cholesterol, phytosterol/octyldodecanol lauroyl glutamate.

In some embodiments of the invention, the active agent comprises one or more of ceramide NP, vitronectin, retinol, retinoid, retinoic acid, palmitoyl tripeptide-1, blue copper peptide, acetyl hexapeptide-8, snake venom peptide, conopeptide, ceramide, ergothioneine, astaxanthin, asiaticoside, sodium hyaluronate, ectoin, nicotinamide, arbutin, vitamin C, tranexamic acid, glutathione, phenethyl resorcinol, salicylic acid, and fruit acid.

In some embodiments of the invention, the composition of the embedded bird's nest peptide comprises, based on 100 parts by weight: 0.1 to 10 parts by weight of bird's nest peptide, 0.5 to 8 parts by weight of hydrogenated lecithin, 10 to 40 parts by weight of polydimethylsiloxane, 0.5 to 5 parts by weight of caprylic/capric triglyceride, 20 to 60 parts by weight of glycerin, 1 to 3 parts by weight of 1, 2-hexanediol, 0.1 to 5 parts by weight of phytosterol/octyldodecanol lauroyl glutamate, 0.001 to 0.5 part by weight of ceramide NP, 0.01 to 0.5 part by weight of stearic acid, 0.01 to 0.5 part by weight of palmitic acid, 0.01 to 0.5 part by weight of cholesterol, 0 to 5 parts by weight of the emulsifier, 0 to 10 parts by weight of the emollient, 0 to 10 parts by weight of the active substance, and the balance of water.

In some embodiments of the invention, the composition comprising, based on 100 parts by weight of the embedded bird's nest peptide: 0.1 to 5 parts by weight of the emulsifier, 0.1 to 10 parts by weight of the emollient, and 0.1 to 10 parts by weight of the active substance.

In some embodiments of the invention, the particle size of the particles in the composition of embedding bird's nest peptide is 50-300 nm.

In some embodiments of the invention, the relative molecular mass of the bird's nest peptide is no greater than 3000.

In some embodiments of the present invention, the bird's nest peptide is added in the form of powder, and the preparation method of the bird's nest peptide powder includes: mixing nidus Collocaliae with water, stewing, and homogenizing to obtain nidus Collocaliae pulp; performing enzymolysis reaction on the bird's nest pulp under the condition that the pH value is 5-9; inactivating enzyme of the bird's nest slurry after the enzymolysis reaction; centrifuging the enzyme-deactivated bird's nest slurry to obtain a bird's nest peptide crude sample solution; filtering the coarse sample solution of the bird's nest peptide by adopting an ultrafiltration membrane with the wavelength of 50-300 nm to obtain a bird's nest peptide solution; concentrating and drying the bird's nest peptide to obtain bird's nest peptide powder.

In some embodiments of the present invention, before stewing and homogenizing the bird's nest further comprises: and cleaning the bird's nest.

In some embodiments of the present invention, the bird's nest and water are mixed according to a mass ratio of (0.1-0.5): 1, mixing and stewing, wherein the stewing time is 0.5-2 h, and the homogenizing pressure is 10-30 Mpa.

In some embodiments of the invention, sodium carbonate is used to adjust the pH of the bird's nest slurry to 5-9.

In some embodiments of the present invention, the bird's nest slurry is subjected to an enzymatic reaction using an alkaline protease, the enzymatic reaction being performed at a temperature of 40-60 for a time of 1-6 hours.

In some embodiments of the invention, the temperature of the enzyme deactivation treatment is 80-100 for 10-30 min.

In some embodiments of the invention, the rotational speed of the centrifugation is 10000 to 15000rpm/min.

In a second aspect of the present invention, the present invention provides a method of preparing a composition of the above-described embedded bird's nest peptide. According to an embodiment of the invention, the method comprises:

dissolving hydrogenated lecithin in glycerol according to a preset ratio to obtain a solution A;

Dissolving the bird's nest peptide into water according to a preset ratio to obtain a solution B;

Mixing and dissolving polydimethylsiloxane, caprylic/capric triglyceride, 1, 2-hexanediol, phytosterol/octyldodecanol lauroyl glutamate, ceramide NP, stearic acid, palmitic acid and cholesterol according to a preset proportion to obtain a solution C;

mixing the solution A, the solution B and the solution C according to a preset proportion to obtain an ABC mixed phase;

And carrying out high-pressure emulsification and homogenization treatment on the ABC mixed phase at a pressure of not less than 500bar and a homogenization speed of not less than 15000rpm/min to obtain the composition for embedding the bird's nest peptide.

The method for preparing the composition for embedding the bird's nest peptide has at least the following beneficial effects:

(1) By adopting the technology of embedding the bird's nest peptide supermolecule liposome, the liposome with a phospholipid bilayer structure generated by hydrogenated lecithin can simultaneously embed and carry hydrophilic and lipophilic functional components, and unstable active substances such as bird's nest peptide are captured inside the liposome and transferred into the skin, so that the functional property of the product is improved. Meanwhile, a better particle size distribution effect can be generated by adopting a high-pressure homogenization technology, supermolecule inclusion with the size of hundred nano particles can be obtained, the transdermal absorption of substances is promoted, and the stability of the product is enhanced;

(2) Compared with the common liposome encapsulation technology and process, such as a film hydration method, a reverse phase evaporation method, a solvent injection method and the like, toxic organic solvents are used, and the encapsulation rate of water-soluble substances is generally 10-15%, the method avoids the use of toxic organic solvents, and improves the encapsulation rate of water-soluble substances such as bird's nest peptide; compared with the common mechanical stirring method, the method has better particle size distribution effect, can obtain supermolecule inclusion with hundred nano particle size, and has stronger stability;

(3) The transdermal absorption of the bird's nest peptide is promoted by the supermolecular liposome encapsulation technology.

(4) By further combining ceramide NP, polydimethylsiloxane, caprylic/capric triglyceride, glycerol, 1, 2-hexanediol, phytosterol/octyldodecanol lauroyl glutamate, stearic acid, palmitic acid, cholesterol, the composition has better moisturizing, whitening, anti-aging and other effects;

(5) The composition meets the given requirements by comprehensively controlling the dosages of all the components and water, is not only beneficial to the dispersion of the bird's nest peptide and all the components and improves the stability of the composition, but also is beneficial to further improving the effects of moisturizing, whitening, resisting aging and the like of the composition.

In some embodiments of the invention, when preparing the solution a, further comprising: an emulsifier is dissolved in the glycerol.

In some embodiments of the invention, when formulating the C solution, the mixed dissolution further comprises: an emollient and/or active is added.

In some embodiments of the invention, the ABC mixed phase is formulated by mixing the solution a and the solution B and then mixing the mixed phase with the solution C.

In some embodiments of the invention, the ABC hybrid phase is high pressure emulsified 1-5 times.

In some embodiments of the invention, the pressure at which the ABC mixed phase is emulsified under high pressure is 700 to 1200bar.

In some embodiments of the invention, the speed of homogenizing the ABC hybrid phase is 15000 to 20000rpm/min.

In some embodiments of the present invention, the dissolution is performed under stirring conditions, the temperature of the dissolution is not lower than 70 , the stirring speed is 300 to 500rpm/min, and the time is 0.5 to 1.5 hours.

In some embodiments of the invention, the dissolving is performed under heating when the B solution is formulated.

In some embodiments of the invention, the dissolving is performed under stirring conditions, the temperature of the dissolving is 90-180 , the stirring speed is 300-500 rpm/min, and the time is 0.5-1.5 h.

In some embodiments of the invention, the ABC mixed phase is prepared by mixing the A solution and the B solution and stirring at a speed of 300-500 rpm/min for 0.5-1.5 hours, and then mixing the A solution and the B solution with the C solution and stirring at a speed of 300-500 rpm/min for 0.5-1.5 hours at a temperature of not less than 50 .

In some embodiments of the invention, the temperature of the high pressure emulsification and the homogenization treatment is from 20to 80 .

In a third aspect of the present invention, the present invention provides a skin care product. According to an embodiment of the invention, the skin care product comprises the composition for embedding the bird's nest peptide and/or the composition for embedding the bird's nest peptide prepared by the method for preparing the composition for embedding the bird's nest peptide. Features and effects described for the above-described composition for embedding bird's nest peptide and the method for preparing the same are also applicable to the skin care product, and will not be described here. In general, the skin care product not only can promote the percutaneous absorption of substances, but also has better stability, and also has the effects of moisturizing, whitening, resisting aging and the like.

Drawings

Fig. 1 is a flow chart of a method of preparing a bird's nest peptide powder according to one embodiment of the present invention.

Fig. 2 is a flow chart of a method of preparing a composition of embedded bird's nest peptide according to one embodiment of the present invention.

FIG. 3 is a graph showing the comparison of DPPH radical scavenging ability test of the sample solutions prepared in test example 1 according to the present invention and obtained by re-dissolving the obtained powder of the bird's nest peptide into different concentrations of the bird's nest peptide by mass.

FIG. 4 is a graph showing comparison of the ABTS free radical scavenging ability test of the inventive test example 1 in which the obtained powder of the bird's nest peptide was reconstituted into sample solutions having different bird's nest peptide mass concentrations.

FIG. 5 is a graph showing the effect of redissolving the obtained bird's nest peptide powder into a sample solution with a bird's nest peptide concentration of 250 g/mL on improving the deformity of the tail fin of the zebra fish irradiated by ultraviolet rays in test example 1 of the present invention.

FIG. 6 is a graph showing comparison of DPPH radical scavenging ability test of sample solutions obtained by diluting the liposome-encapsulated bird's nest peptide composition prepared in test example 2 according to the present invention to different bird's nest peptide concentrations.

FIG. 7 is a graph showing comparison of the ABTS radical scavenging ability test of the liposome-encapsulated bird's nest peptide composition prepared in test example 2 diluted into sample solutions of different bird's nest peptide concentrations.

FIG. 8 is a graph showing the effect of diluting the liposome-encapsulated bird's nest peptide composition prepared in test example 2 of the present invention to a sample solution having a bird's nest peptide concentration of 250. Mu.g/mL on improvement of the tail fin deformity of a zebra fish by ultraviolet irradiation.

FIG. 9 is a graph showing the comparison of the results of the accelerated stability test at different temperatures before and after diluting the liposome-encapsulated bird's nest peptide composition prepared in test example 2 according to the present invention.

FIG. 10 is a graph showing the particle size distribution of particles in the liposome-encapsulated bird's nest peptide composition prepared in test example 2 of the present invention.

FIG. 11 is a transmission electron microscope image of a composition for preparing liposome-encapsulated bird's nest peptide according to test example 2 of the present invention on a scale of 200 nm.

FIG. 12 is a transmission electron microscope image of a composition for preparing liposome-encapsulated bird's nest peptide according to test example 2 of the present invention on a scale of 100 nm.

FIG. 13 is a Zeta potential test chart of a liposome-encapsulated bird's nest peptide composition prepared in test example 2 of the present invention.

FIG. 14 is a Raman spectrum of a liposome-encapsulated bird's nest peptide composition prepared in test example 2 of the present invention.

FIG. 15 is a transdermal test chart of a liposome-encapsulated bird's nest peptide composition prepared in test example 2 of the present invention.

FIG. 16 is a graph showing the effect of improving the deformation of the tail fin of a zebra fish by ultraviolet irradiation after diluting the liposome-encapsulated bird's nest peptide compositions prepared in test example 3 and test example 4 to a bird's nest peptide concentration of 100. Mu.g/mL.

FIG. 17 is a graph showing the effect of improving the deformation of the tail fin of a zebra fish by ultraviolet irradiation after diluting the liposome-encapsulated bird's nest peptide compositions prepared in test example 3 and test example 4 to a bird's nest peptide concentration of 250. Mu.g/mL.

Detailed Description

Embodiments of the present invention are described in detail below, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to like or similar elements or elements having like or similar functions throughout. The embodiments described below by referring to the drawings are illustrative and intended to explain the present invention and should not be construed as limiting the invention.

In a first aspect of the invention, the invention provides a composition for embedding bird's nest peptide. According to an embodiment of the invention, the composition comprises: 0.1 to 10 parts by weight of bird's nest peptide, 0.5 to 8 parts by weight of hydrogenated lecithin, 10 to 40 parts by weight of polydimethylsiloxane, 0.5 to 5 parts by weight of caprylic/capric triglyceride, 20 to 60 parts by weight of glycerin, 1 to 3 parts by weight of 1, 2-hexanediol, 0.1 to 5 parts by weight of phytosterol/octyldodecanol lauroyl glutamate, 0.001 to 0.5 part by weight of ceramide NP, 0.01 to 0.5 part by weight of stearic acid, 0.01 to 0.5 part by weight of palmitic acid, 0.01 to 0.5 part by weight of cholesterol, and not more than 68 parts by weight of water, wherein the hydrogenated lecithin is embedded with the bird's nest peptide.

Illustratively, the bird's nest peptide may be 0.1, 1,3, 5, 7, 9 or 10 parts by weight, etc. in the composition embedding the bird's nest peptide; the hydrogenated lecithin may be 0.5, 2, 4, 6 or 8 parts by weight, etc.; the polydimethyl siloxane can be 10, 15, 20, 25, 30, 35 or 40 parts by weight, etc.; the weight fraction of caprylic/capric triglyceride may be 0.5, 1,2,3, 4 or 5, etc.; the parts by weight of glycerin may be 20, 25, 30, 35, 40, 45, 50, 55 or 60, etc.; the weight parts of 1, 2-hexanediol can be 1, 1.5, 2, 2.5 or 3, etc.; the weight fraction of phytosterol/octyldodecanol lauroyl glutamate may be 0.1, 1,2,3, 4 or 5, etc.; the parts by weight of ceramide NP may be 0.001, 0.01, 0.05, 0.1, 0.2, 0.3, 0.4, or 0.5, etc.; the weight fraction of stearic acid may be 0.01, 0.05, 0.1, 0.2, 0.3, 0.4 or 0.5, etc.; the weight fraction of palmitic acid may be 0.01, 0.05, 0.1, 0.2, 0.3, 0.4 or 0.5, etc.; the cholesterol may be 0.01, 0.05, 0.1, 0.2, 0.3, 0.4 or 0.5 parts by weight, etc.; the water may be 68, 60, 50, 40, 30, 20, 10 or 5 parts by weight, etc.

In some embodiments of the present invention, the water in the composition embedding the bird's nest peptide may be deionized water, purified water, highly purified water, analytically pure water, or the like, whereby the possibility of introducing impurities may be further reduced.

In some embodiments of the present invention, the composition for embedding bird's nest peptide may further include, on the basis of satisfying the above original composition and ratio: 0 to 5 weight portions of emulsifying agent, 0 to 10 weight portions of emollient and 0 to 10 weight portions of active substance. For example, 0.1, 0.5, 1, 2,3, 4, or 5 parts by weight of an emulsifier may be included, and for another example, 0.1, 0.5, 1,3,5, 7, 9, or 10 parts by weight of an emollient may be included, and for another example, 0.1, 0.5, 1, 2,4,6, 8, or 10 parts by weight of an active may be included. Alternatively, the levels of emulsifier, emollient, and active may each be non-zero or not simultaneously zero. By further adding the emulsifier, the emollient and the active substances in the content in the composition embedded with the bird's nest peptide, the composition is beneficial to further promoting the effects of moisturizing, whitening, resisting aging and the like of the composition, and the type and the amount of the active substances can be selected according to the expected functional effect.

In some embodiments of the invention, the emulsifier may include, but is not limited to, one or more of lecithin, hydrogenated lecithin, palmitic acid, stearic acid, olive oil PEG-7 esters, cholesterol, PEG-8 caprylic/capric glycerides, phytosterol/canola glycerides, sorbitan olive oleate, polysorbate-20, laureth-23, PEG-40 stearate, steareth-21, palmitols and PEG-75 stearate and a mixture of glyceryl stearate and steareth-20, oleyl erucate, polysorbate-80, a mixture of glyceryl stearate and PEG-75 stearate, C12-16 alcohol, octyldodecanol, PEG-30 dimer hydroxystearate, cetostearyl ether-25. It is understood that the emulsifier is additionally added on the basis of meeting the original composition and proportion of the composition for embedding the bird's nest peptide.

In some embodiments of the present invention, the emollient may include, but is not limited to, one or more of dimethicone, 1, 2-hexanediol, 1, 3-butylene glycol, 1, 2-pentanediol, caprylic/capric triglyceride, dimethiconol, isododecane, isohexadecane, squalane, half angle squalane, cetostearyl alcohol isononanoate, oleic acid capric acid, myristyl caprate, octyldodecanol, mineral oil, ethylhexyl stearate, coco-caprylate/caprate, shea butter, rapeseed oil, polydecene, shea butter, soybean oil, olive oil, palmitic acid, stearic acid, cholesterol, phytosterol/octyldodecanol lauroyl glutamate. It is understood that the emollient is additionally added on the basis of meeting the original composition and proportion of the composition for embedding the bird's nest peptide.

In some embodiments of the invention, the active may include, but is not limited to, one or more of ceramide NP, vitronectin, retinol, retinoid, retinoic acid, palmitoyl tripeptide-1, blue copper peptide, acetyl hexapeptide-8, snake venom peptide, conopeptide, ceramide, ergothioneine, astaxanthin, asiaticoside, sodium hyaluronate, ectoin, nicotinamide, arbutin, vitamin C, tranexamic acid, glutathione, phenethyl resorcinol, salicylic acid, and fruit acid. It is understood that the active substances are additionally added on the basis of meeting the original composition and proportion of the composition for embedding the bird's nest peptide.

In some embodiments of the present invention, the composition based on 100 parts by weight of the embedded bird's nest peptide may include: 0.1 to 10 parts by weight of bird's nest peptide, 0.5 to 8 parts by weight of hydrogenated lecithin, 10 to 40 parts by weight of polydimethylsiloxane, 0.5 to 5 parts by weight of caprylic/capric triglyceride, 20 to 60 parts by weight of glycerin, 1 to 3 parts by weight of 1, 2-hexanediol, 0.1 to 5 parts by weight of phytosterol/octyldodecanol lauroyl glutamate, 0.001 to 0.5 part by weight of ceramide NP, 0.01 to 0.5 part by weight of stearic acid, 0.01 to 0.5 part by weight of palmitic acid, 0.01 to 0.5 part by weight of cholesterol, 0 to 5 parts by weight of emulsifier, 0 to 10 parts by weight of emollient, 0 to 10 parts by weight of active substance, and the balance water. The composition with embedded bird's nest peptide has the material composition and proportion meeting the given range, and is favorable to promote transdermal absorption of matter, raise the stability of the composition, and make the composition possess the functions of moistening, whitening, resisting aging, etc.

In some embodiments of the present invention, the composition based on 100 parts by weight of the embedded bird's nest peptide may include: 0.1 to 5 parts by weight of an emulsifier, 0.1 to 10 parts by weight of an emollient, and 0.1 to 10 parts by weight of an active substance. Therefore, the composition is beneficial to further promoting the effects of moisturizing, whitening, resisting aging and the like of the composition, and the types and the dosage of the active substances can be selectively regulated according to the expected functional effect.

In some embodiments of the present invention, the particle size of the composition of the embedded bird's nest peptide may be 50-300 nm, for example, the particle size of the composition of the embedded bird's nest peptide may be 100-200 nm, 50nm, 100nm, 150nm, 200nm, 250nm or 300nm, etc., so that the particle size of the composition of the embedded bird's nest peptide satisfies the given range, which not only can make the composition have stronger stability, but also can further promote the transdermal absorption of substances. Wherein, the particle size of the bird's nest peptide composition can be controlled between 50nm and 300nm by high-pressure emulsification and homogenization and ultrafiltration by an ultrafiltration membrane.

In some embodiments of the invention, the relative molecular mass of the bird's nest peptide may be no greater than 3000, e.g., the relative molecular mass of the bird's nest peptide may be no greater than 3000, 2500, 2000, 1500, 1000, 800, 500, etc. The small molecule bird's nest peptide is not only beneficial to improving the dispersibility of the bird's nest peptide in the composition and reducing the particle size of particles in the composition, so that the composition has stronger stability, but also can further promote the percutaneous absorption of the substance bird's nest peptide.

In some embodiments of the present invention, the encapsulation rate of the hydrogenated lecithin to the liposome may be not less than 45%, such as not less than 50%, 55%, 60% or 65%, etc., whereby the transdermal absorption of the active substance bird's nest peptide may be further promoted.

In some embodiments of the present invention, the bird's nest peptide may be added in the form of powder, and the preparation method of the bird's nest peptide powder may include the steps of:

(a) Mixing nidus Collocaliae with water, stewing, and homogenizing to obtain nidus Collocaliae pulp

According to the embodiment of the invention, edible bird's nest raw materials can be adopted.

In some embodiments of the present invention, stewing and homogenizing the bird's nest may further include: the bird's nest is cleaned, and water can be added for cleaning. The bird's nest is cleaned, so that impurity introduction can be reduced.

In some embodiments of the present invention, the bird's nest and water may be mixed in a mass ratio of (0.1-0.5): 1, and the stewing and boiling time can be 0.5-2 h, and the homogenizing pressure can be 10-30 Mpa. For example, the cleaned edible bird's nest raw material and water can be compared with feed liquid with the mass ratio of 0.1/1, 0.2/1, 0.3/1, 0.4/1 or 0.5/1 and the like in a jacketed kettle for stewing for 0.5h, 1h, 1.5h or 2h and the like, and then the stewed edible bird's nest can be homogenized under the homogenizing pressure condition of 10MPa, 15MPa, 20MPa, 25MPa or 30MPa and the like, and the homogenizing time can be about 30min, so as to obtain the edible bird's nest slurry. Wherein, the control of the feed liquid ratio, stewing conditions and homogenizing pressure is beneficial to improving the uniformity of the bird nest slurry.

(B) The edible bird nest pulp is subjected to enzymolysis reaction under the condition that the pH value is 5-9

According to the embodiment of the invention, the homogenized bird's nest pulp can be transferred into an enzymolysis tank, the enzymolysis reaction is carried out after the pH value is regulated, the relative molecular mass of the bird's nest peptide can be reduced by carrying out the enzymolysis reaction on the bird's nest pulp, and the small molecular bird's nest peptide can be obtained. The pH value of the enzymolysis reaction can be 5,6, 7, 8 or 9, etc.

In some embodiments of the present invention, sodium carbonate may be used to adjust the pH of the bird's nest slurry to 5-9.

In some embodiments of the present invention, the bird's nest slurry may be subjected to an enzymatic reaction using alkaline protease at a temperature of 40-60 for a time of 1-6 hours. As some specific examples, the alkaline protease may be used in an amount of 0.01 to 0.05% by mass, for example, 0.01%, 0.02%, 0.03%, 0.04%, or 0.05% by mass, based on the mass of the bird's nest peptide slurry, and the like. By controlling the enzymolysis conditions, quick and full fracture of chain segments of the bird's nest peptide is facilitated, and the small molecule bird's nest peptide is obtained.

(C) Enzyme-inactivating treatment is carried out on the bird's nest slurry after the enzymolysis reaction

According to embodiments of the present invention, the enzyme may be deactivated by performing an enzyme deactivation treatment. In some embodiments of the invention, the temperature of the enzyme deactivation treatment may be 80-100and the time may be 10-30 minutes. After the enzymolysis is finished, the temperature of the system can be adjusted to 80-100 and kept for 10-30 min, so that the alkaline protease can be fully deactivated.

(D) Centrifuging the enzyme-deactivated nidus Collocaliae slurry to obtain crude nidus Collocaliae peptide sample solution

In some embodiments of the present invention, the enzyme-deactivated nidus Collocaliae slurry is cooled and then subjected to centrifugation, and the rotational speed of the centrifugation may be 10000-15000 rpm/min, such as 10000rpm/min, 12000rpm/min, 15000rpm/min, etc.

(E) Filtering the coarse sample solution of the bird's nest peptide by adopting an ultrafiltration membrane with the wavelength of 50-300 nm to obtain a bird's nest peptide solution

According to the embodiment of the invention, the ultrafiltration membrane is adopted to filter the coarse sample solution of the bird's nest peptide, so that the bird's nest peptide solution with hundred-nanometer particle size range distribution is obtained, the transdermal absorption of the composition is promoted, and the stability of the composition is enhanced.

(F) Concentrating and drying nidus Collocaliae peptide to obtain nidus Collocaliae peptide powder

According to the embodiment of the invention, the cubilose peptide solution is concentrated by 3-8 times through membrane concentration, rotary evaporation and other modes; and then spray drying the concentrated solution, wherein the air inlet temperature can be controlled to be 150-200 and the air outlet temperature is more than or equal to 70 during spray drying, so as to obtain the bird's nest peptide powder.

The small molecule bird's nest peptide powder can be obtained by performing the operations of stewing, pulping and homogenizing, regulating pH, enzymolysis, enzyme deactivation, cooling, centrifugation, ultrafiltration, concentration, drying and the like on the bird's nest peptide.

(i) Dissolving hydrogenated lecithin in glycerol according to a preset proportion to obtain solution A

In some embodiments of the present invention, the dissolution may be performed under stirring conditions, the temperature of the dissolution may be not lower than 70 , the stirring speed may be 300 to 500rpm/min, and the time may be 0.5 to 1.5 hours. For example, 0.5 to 8 parts by weight of hydrogenated lecithin and 20 to 60 parts by weight of glycerin may be weighed in proportion, glycerin may be heated to 70or higher, hydrogenated lecithin may be added to dissolve, and stirred at 300 to 500rpm/min for 0.5 to 1.5 hours to mix uniformly, whereby a uniform A solution may be obtained.

In some embodiments of the present invention, when preparing the solution a, it may further include: the emulsifier is dissolved in glycerin. When other emulsifier components are additionally added into the composition for embedding the bird's nest peptide, the emulsifier can be dissolved in the glycerin, for example, 20-60 parts by weight of glycerin can be heated to more than 70 , and then no more than 5 parts by weight of emulsifier can be further added. The amount of the emulsifier and the optional range are described in detail in the foregoing sections, and are not repeated here.

(Ii) Dissolving nidus Collocaliae peptide in water according to preset proportion to obtain solution B

In some embodiments of the present invention, the dissolution may be performed under heating conditions, which may be about 50 , for example, (505) c, etc., when preparing the solution B, thereby further facilitating rapid, uniform dispersion of the bird's nest peptide in water. For example, after 0.1 to 10 parts by weight of the bird's nest peptide is mixed with water, the water is heated to 50to dissolve, and stirring may be continued at a rotation speed of 300 to 500rpm/min to ensure complete dissolution. In the dissolving process, water and the bird's nest peptide can be heated to a preset temperature for dissolving after being mixed, or the bird's nest peptide can be added for mixing after the water is heated to the preset temperature.

In addition, the bird's nest peptide can be added in the form of bird's nest slurry or powder, and when the operation is performed according to the preset proportion, the amount of the bird's nest peptide is calculated according to the mass of dry powder of the bird's nest peptide, wherein the optional range of the bird's nest peptide and water and the amount of water are described in detail in the foregoing parts, and the details are not repeated here.

(Iii) Mixing and dissolving polydimethylsiloxane, caprylic/capric triglyceride, 1, 2-hexanediol, phytosterol/octyldodecanol lauroyl glutamate, ceramide NP, stearic acid, palmitic acid and cholesterol according to a preset proportion to obtain a solution C

In some embodiments of the present invention, the dissolution may be performed under stirring conditions, the temperature of the dissolution may be 90 to 180 , the stirring speed may be 300 to 500rpm/min, and the time may be 0.5 to 1.5 hours. For example, 10 to 40 parts by weight of polydimethylsiloxane, 0.5 to 5 parts by weight of caprylic/capric triglyceride, 1 to 3 parts by weight of 1, 2-hexanediol, 0.1 to 5 parts by weight of phytosterol/octyldodecanol lauroyl glutamate, 0.001 to 0.5 part by weight of ceramide NP, 0.01 to 0.5 part by weight of stearic acid, 0.01 to 0.5 part by weight of palmitic acid, and 0.01 to 0.5 part by weight of cholesterol may be mixed, heated to 90 to 180and stirred at 300 to 500rpm/min for 0.5 to 1.5 hours, and stirred, whereby a uniform C solution may be obtained. Alternatively, the heating temperature may also be preferably 90 to 140 .

In some embodiments of the invention, when preparing the solution C, the mixed dissolution may further include: an emollient and/or active is added. When additional emollients and ingredients are desired in the bird's nest peptide-embedding composition, no more than 10 parts by weight of emollients and/or no more than 10 parts by weight of active substances may be further added when emulsifiers and/or active substances may be added. The amounts and optional ranges of the emulsifying agent and the active substance are described in detail in the foregoing sections, and are not repeated here.

In addition, in the present invention, the heating method and stirring method used in the dissolution operation of each step are not particularly limited, and for example, a conventional heating device may be selected for heating, for example, a muffle furnace, a water bath, an oil bath, a dry-type thermostat, an electrothermal blowing drying oven, an electrothermal jacket, an electrothermal plate, etc. may be selected for heating; for example, the stirring may be performed by a conventional stirring device, such as a magnetic stirrer, a push rod stirrer, an impeller stirrer, a homogenizing stirrer, a heat dissipation stirrer, an Ultra stirrer, or the like.

The order of steps (i), (ii) and (iii) is not particularly limited, and may be performed sequentially, or in parallel, and (i), (ii) and (iii) should not be construed as limiting the order.

(Iv) Mixing the solution A, the solution B and the solution C according to a preset proportion to obtain an ABC mixed phase

In some embodiments of the invention, the ABC mixed phase is prepared by mixing solutions a and B and then mixing with solution C. For example, the solution A and the solution B can be mixed and stirred for 0.5 to 1.5 hours at the rotating speed of 300 to 500rpm/min to obtain a homogenized AB mixed solution; and mixing the AB mixed solution and the C solution, and stirring for 0.5-1.5 h at the temperature of not lower than 50 and the rotating speed of 300-500 rpm/min. Thus, it is advantageous to further improve the ABC mixed phase uniformity.

(V) And (3) carrying out high-pressure emulsification and homogenization treatment on the ABC mixed phase at a pressure of not less than 500bar and a homogenization speed of not less than 15000rpm/min to obtain the composition for embedding the bird's nest peptide.

According to the embodiments of the present invention, by performing the high-pressure emulsification and homogenization treatment under the given conditions, a nanodispersion composition containing bird's nest peptide dispersion particles can be obtained. The temperature of the high-pressure emulsification and homogenization treatment may be set to 20 to 80 from the viewpoints of stabilization of the bird's nest peptide and miniaturization of the ceramide dispersion particles. The high-pressure emulsification and the homogenization can be performed sequentially or simultaneously.

In some embodiments of the invention, the ABC hybrid phase may be emulsified 1 to 5 times, preferably 2 to 5 times. The high-pressure emulsification treatment is carried out for more than 2 times, so that the uniformity of the whole liquid system can be further improved.

In some embodiments of the invention, the pressure at which the ABC mixed phase is emulsified at high pressure may be 700 to 1200bar. For example, 700 to 1100bar, 800bar, 900bar, 1000bar, 900 to 1200bar, etc. The pressure of the homogenizing treatment is the same as the pressure of the high-pressure emulsification. The emulsification and homogenization pressures added to the dispersed material are more advantageous over 700bar to achieve nanodispersion of the dispersed particles in the composition system, preferably over 800bar, over 900bar or over 1000 bar. In addition, the inventor finds that the encapsulation rate of liposome to the nidus Collocaliae peptide in the finally prepared composition is in a trend of rising firstly and then tending to be stable along with the increase of emulsification and homogenization pressure, and the emulsification and homogenization pressure is controlled between 700 and 1200bar, so that the nidus Collocaliae peptide has higher encapsulation rate.

In some embodiments of the present invention, the speed of homogenizing the ABC mixed phase may be 15000-20000 rpm/min, thereby further facilitating the improved nanodispersion of the dispersed particles in the composition system, resulting in a higher encapsulation rate of the bird's nest peptide.

In some embodiments of the invention, the temperature of the high pressure emulsification and homogenization treatment is from 20 to 80 . When the high-pressure emulsification and homogenization are performed under heating conditions, the method may further include cooling the high-pressure emulsification and homogenization system, followed by a repacking process.

In summary, the method for preparing the composition for embedding the bird's nest peptide according to the above embodiment of the present invention has at least the following advantages:

The following examples are illustrative only and are not to be construed as limiting the application. The examples are not to be construed as limiting the specific techniques or conditions described in the literature in this field or as per the specifications of the product.

Test example 1

The experimental example has no supermolecule liposome encapsulation step and is used for confirming the efficacy of the bird's nest peptide.

Step one: preparation of nidus Collocaliae peptide

(1) Cleaning the bird's nest raw material: weighing a certain amount of edible bird's nest raw material, and adding water for cleaning;

(2) Stewing: the cleaned bird nest raw material and water are placed in a jacketed kettle according to the mass ratio of 0.3:1 to be stewed for 2 hours in boiling water;

(3) Pulping and homogenizing: homogenizing the stewed edible bird's nest into bird's nest pulp under the condition of 30 MPa;

(4) Adjusting the pH: pouring homogenized nidus Collocaliae slurry into an enzymolysis tank, and regulating pH of the system to 9.0 with sodium carbonate;

(5) Enzymolysis: adding 6000U/g alkaline protease into an enzymolysis tank for enzymolysis reaction for 6 hours; the enzymolysis temperature is kept at 60 , and after the enzymolysis is finished, the system temperature is regulated to 100 and kept for 30min to sufficiently inactivate enzyme;

(6) And (3) centrifuging: centrifuging the enzymolysis liquid sample under the condition of 13000rpm/min to obtain edible bird's nest peptide crude sample solution;

(7) Ultrafiltration: ultrafiltering the edible bird's nest peptide coarse sample solution by using a 200nm ultrafiltration membrane to obtain a bird's nest peptide solution;

(8) Concentrating: concentrating the bird's nest peptide solution by 8 times;

(9) And (3) drying: and (3) spray-drying the bird's nest peptide concentrated solution, wherein the air inlet temperature is 170 , and the air outlet temperature is more than 70 , so as to obtain bird's nest peptide powder.

Test example 1, evaluation of antioxidant, photo-aging (anti-wrinkle) efficacy of bird's nest peptide:

The testing method comprises the following steps:

DPPH radical scavenging ability test: the bird's nest peptide powder sample prepared in the test example 1 is respectively redissolved into sample solutions with required solubility shown in fig. 3, 2mL of sample solutions with different concentrations are respectively taken, 2mL of DPPH solution is added, the mixture is uniformly mixed, and after the mixture is placed for 30min at room temperature, the mixture is centrifuged for 10min at 5000 r/min. The supernatant was taken and absorbance was measured at 517 nm.

DPPH radical scavenging was calculated according to the following formula:

Wherein,

A0: absorbance value of 2mL absolute ethanol+2 mL DPPH radical solution;

a1: absorbance value of 2mL sample solution+2 mL DPPH radical solution;

A2: absorbance value of 2mL sample solution+2 mL absolute ethanol.

Test results: the DPPH free clearance ability of sample solutions with different bird's nest peptide mass concentrations is shown in figure 3, and the bird's nest peptide has an antioxidation effect and is concentration dependent as shown in figure 3.

ABTS free radical scavenging ability test: 7mmol/L ABTS aqueous solution and 2.45mmol/L potassium persulfate are mixed at a ratio of 1:1 (v/v) and reacted at room temperature for 12-16 h in the absence of light. Then diluted with 1mmol/L PBS to give an absorbance at 734nm of 0.70.+ -. 0.02. The bird's nest peptide powder sample prepared in test example 1 is respectively redissolved into sample solutions with required solubility shown in fig. 4, 0.4mL is taken, 4mL of diluted ABTS ⁺. Solution is added, absorbance A ₁ is measured at 734nm after light-shielding reaction is carried out for 6min, absorbance A ₂ is measured by taking distilled water as a blank group instead of ABTS ⁺. Solution, and absorbance A ₀ is measured by taking distilled water as a control group instead of sample.

ABTS ⁺ clearance was calculated as follows:

/>

Wherein,

A ₀: absorbance value of 0.4mL ultrapure water+4 mL ABTS radical solution;

A ₁: absorbance value of 0.4mL sample solution+4 mL ABTS radical solution;

a ₂: absorbance value of 0.4mL sample solution+4 mL ultrapure water.

Test results: the ABTS of the sample solutions with different bird's nest peptide mass concentrations is shown in fig. 4, and the bird's nest peptide has an antioxidant effect and is concentration dependent, which is consistent with DPPH results, as shown in fig. 4.

Zebra fish anti-photoaging (anti-wrinkle) efficacy test:

maximum Tolerance Concentration (MTC): MTC was determined as a high concentration group of 3dpf zebra fish larvae without any death (no heartbeats) and other toxic effects (pericardial edema, trunk flexion, no response to mechanical stimulus, unclear muscle texture, etc.).

Evaluation of anti-photoaging (anti-wrinkle) efficacy:

Zebra fish larvae with normal 2dpf are selected, the zebra fish larvae are divided into three groups, namely a normal control group, a model control group and a test sample group, and are randomly distributed into 6-hole cell culture plates, the embryo culture solution in the 6-hole cell culture plates is removed under the condition that the larvae are not damaged by 15 tails per hole, 3mL of embryo culture solution is added into the normal control group and the model control group, and 3mL of prepared bird's nest peptide solution (the concentration of bird's nest peptide is 50 mug/mL, 100 mug/mL and 250 mug/mL) is added into the test sample group. Firstly, after fully and uniformly mixing, a culture plate cover plate is covered, and the culture plate is incubated for 2 hours in a dark place. Then, three irradiation times were performed with ultraviolet rays for 30 seconds at intervals of 30 minutes. Each time, the irradiation dose was 243556.45 + -0.14 J/cm ², and the total irradiation dose was 730669 + -0.542 J/cm ². Finally, the incubation is continued for 22 hours in a constant temperature incubator at 28+/-0.5 in a dark place, and the observation is carried out by using a biological microscope.

Image analysis: and after photographing is completed, analyzing the obtained zebra fish pictures by using image analysis software.

Test results:

Maximum Tolerance Concentration (MTC): when the mass concentration of the bird's nest peptide is 50 mug/mL, 100 mug/mL and 250 mug/mL, 3mL of young zebra fish does not have any embryonic toxicity symptoms such as death (no heart beat) and other toxic effects (pericardial edema, trunk bending, no response to mechanical stimulus, unclear muscle texture and the like). Thus, 250 g/mL of bird's nest peptide can be used as the highest concentration group.

Ultraviolet-induced contraction of zebra fish tail fins is an ideal regeneration research mode widely used at present and used for researching anti-photoaging intervention. According to the invention, the protection effect of the bird's nest peptide on the zebra fish tail fins under ultraviolet irradiation is examined, and as can be seen from fig. 5, the zebra fish tail fins of a control group (without ultraviolet irradiation) are normal in development, but the zebra fish tail fin model group exposed to ultraviolet rays shows obvious deformity and shrinkage, and the result shows that the zebra fish ultraviolet induced photoaging model is successfully established. The tested sample group obviously improves the malformation of the zebra fish tail fin irradiated by ultraviolet under the intervention of 250 mug/mL of bird's nest peptide.

Test example 2

The test example is used for comparing the molecular particle size of the supermolecule coated bird's nest peptide preparation with the efficacy of the uncoated bird's nest peptide and performing transdermal difference.

Step one: process for preparing bird's nest peptide (same test example 1)

(8) Concentrating: concentrating the bird's nest peptide solution by 8 times;

Step two: preparation of composition of supermolecule-coated bird's nest peptide

The preparation proportion and the process of the emulsion:

Table 1 raw material composition and ratio of the composition for preparing liposome-entrapped bird's nest peptide in test example 2

Component (A)	Mass percent ratio
		Bird's nest peptide	2.5
Purified water	21
		Hydrogenated lecithin	4
Polydimethylsiloxane	20
		Glycerol	47
Caprylic/capric triglyceride	3
		1, 2-Hexanediol	2
Phytosterol/octyldodecanol lauroyl glutamate	0.1
		Ceramide NP	0.1
Stearic acid	0.1
		Palmitic acid	0.1
Cholesterol	0.1

(1) Solution A: weighing 47 parts by weight of glycerol and 4 parts by weight of hydrogenated lecithin according to a proportion, heating the glycerol to above 70 , and adding the hydrogenated lecithin for dissolution; 400 Stirring at rpm/min for 1 hour.

(2) Solution B: weighing 2.5 parts by weight of bird's nest peptide according to a proportion, adding 21 parts by weight of purified water into the bird's nest peptide, and heating to more than 50 for dissolution; stirring at 400 rpm/min at room temperature until the mixture is completely dissolved for later use.

(3) Solution C: weighing 0.1 part by weight of ceramide NP, 0.1 part by weight of stearic acid, 0.1 part by weight of palmitic acid, 0.1 part by weight of cholesterol, 0.1 part by weight of phytosterol/octyl dodecanol lauroyl glutamate, 3 parts by weight of caprylic/capric triglyceride, 20 parts by weight of polydimethylsiloxane and 2 parts by weight of 1, 2-hexanediol according to the proportion, mixing, heating and stirring the components, and heating to 100-140 ; and stirred at 400 rpm/min for 1 hour.

The high-pressure emulsification treatment process comprises the following steps:

(1) Adding the solution A into the solution B, stirring for 1 hour at 400 rpm/min, and uniformly mixing to achieve a homogenization stage;

(2) Adding the solution C into the AB mixed solution, stirring for 1 hour at 400 rpm/min and above 50 and uniformly mixing to achieve the emulsification stage;

(3) Putting the emulsified ABC mixed phase into a high-pressure micro-jet homogenizer, performing high-pressure emulsification treatment, and treating for 5 times under the pressure of 1000 bar; the homogenization speed was 20000 rpm/min, and the temperature was set at 60for 0.5 hours.

(4) And cooling the emulsified mixed phase to 25-40 to obtain a liposome-coated (i.e. embedded) bird nest peptide composition (hereinafter referred to as embedded bird nest peptide or bird nest peptide-coated composition) formed by hydrogenated lecithin.

In test example 2, the obtained composition had an evaluation of antioxidant, photo-aging (anti-wrinkle) efficacy:

The testing method comprises the following steps:

DPPH radical scavenging ability test: the composition prepared in test example 2 is diluted into sample solutions with the required bird's nest peptide mass solubility and the mass concentration of the bird's nest peptide is shown in figure 6, 2mL of sample solutions with different bird's nest peptide mass concentrations are respectively taken, 2mL of DPPH solution is added, the mixture is uniformly mixed, and after the mixture is placed at room temperature for 30min, the mixture is centrifuged at 5000r/min for 10min. The supernatant was taken and absorbance was measured at 517 nm.

DPPH radical scavenging was calculated according to the following formula:

Wherein,

A0: absorbance value of 2mL absolute ethanol+2 mL DPPH radical solution;

a1: absorbance value of 2mL sample solution+2 mL DPPH radical solution;

A2: absorbance value of 2mL sample solution+2 mL absolute ethanol.

Test results: the DPPH free clearance capability of the composition embedded with the bird's nest peptide diluted into the diluted liquid samples with different bird's nest peptide mass concentrations is shown in fig. 6, and the composition coated with the bird's nest peptide has the antioxidation effect and shows concentration dependence.

ABTS free radical scavenging ability test: 7mmol/L ABTS aqueous solution and 2.45mmol/L potassium persulfate are mixed at a ratio of 1:1 (v/v) and reacted at room temperature for 12-16 h in the absence of light. Then diluted with 1mmol/L PBS to give an absorbance at 734nm of 0.70.+ -. 0.02. Diluting the composition embedded with the bird's nest peptide into a sample solution with the required bird's nest peptide mass solubility shown in fig. 7, taking 0.4mL, adding 4mL of diluted ABTS ⁺. Solution, carrying out light-shielding reaction for 6min, measuring absorbance A ₁ at 734nm, using distilled water instead of ABTS ⁺. Solution as a blank group to measure absorbance A ₂, and using distilled water instead of the sample as a control group to measure absorbance A ₀.

ABTS ⁺ clearance was calculated as follows:

Wherein,

A ₀: absorbance value of 0.4mL ultrapure water+4 mL ABTS radical solution;

A ₁: absorbance value of 0.4mL sample solution+4 mL ABTS radical solution;

a ₂: absorbance value of 0.4mL sample solution+4 mL ultrapure water.

Test results: the clearance of ABTS of the dilution liquid samples with different mass concentrations of the bird's nest peptide from the composition embedded with the bird's nest peptide is shown in figure 7, and the composition embedded with the bird's nest peptide by liposome has the antioxidation effect and shows concentration dependence, which is consistent with DPPH results.

Zebra fish anti-photoaging (anti-wrinkle) efficacy test:

Evaluation of anti-photoaging (anti-wrinkle) efficacy

Selecting young zebra fish with 2dpf which are normal in development, dividing the zebra fish into three groups, namely a normal control group, a model control group and a test sample group, randomly distributing the zebra fish into 6-hole cell culture plates, removing embryo culture solution from the 6-hole cell culture plates under the condition of not damaging the young fish by 15 tails per hole, adding 3mL of embryo culture solution into the normal control group and the model control group, and adding 3mL of prepared coated bird's nest peptide solution into the test sample group. Firstly, after fully and uniformly mixing, a culture plate cover plate is covered, and the culture plate is incubated for 2 hours in a dark place. Then, three irradiation times were performed with ultraviolet rays for 30 seconds at intervals of 30 minutes. Each time the irradiation amount is 239986.44 +/-0.13 mu J/cm ², and the total irradiation amount is 730651 +/-0.522 mu J/cm ². Finally, the incubation is continued for 22 hours in a constant temperature incubator at 28+/-0.5 in a dark place, and the observation is carried out by using a biological microscope.

Test results:

Maximum Tolerance Concentration (MTC): when the bird's nest peptide-embedded composition prepared according to test example 2 was diluted into sample solutions with bird's nest peptide mass concentrations of 50 mug/mL, 100 mug/mL and 250 mug/mL, respectively, 3mL of young zebra fish did not show any embryonic toxicity symptoms such as death (no heart beat) and other toxic effects (pericardial edema, trunk bending, no response to mechanical stimulus, unclear muscle texture, etc.). Thus, a composition of embedded bird's nest peptide with a bird's nest peptide mass concentration of 250 g/mL can be used as the highest concentration group.

In this test example 2, the protection effect of the composition for embedding the bird's nest peptide on the tail fin of the zebra fish under ultraviolet irradiation was examined, and the composition for embedding the bird's nest peptide with a mass percentage of 2.5% was diluted to have a bird's nest peptide mass concentration of 250 g/mL (0.25%) for the test, based on the maximum tolerance concentration of the zebra fish. As can be seen from fig. 8, the zebra fish tail fin model group of the control group (without ultraviolet irradiation) developed normally, but the zebra fish tail fin model group exposed to ultraviolet rays showed obvious malformation and shrinkage, and the result shows that the zebra fish ultraviolet induced photoaging model was successfully established. The composition embedded with the bird's nest peptide is remarkably improved by the zebra fish tail fin irradiated by ultraviolet under the intervention of the bird's nest peptide mass concentration of 250 mug/mL. Thus, the encapsulated bird's nest peptide was shown to have anti-photoaging efficacy in vivo as a model organism-zebra fish model result.

In test example 2, stability test of the obtained composition:

Stability acceleration over 4 weeks: the composition prepared in test example 2 (bird's nest peptide concentration 2.5%) and the diluted solution obtained by diluting the composition prepared in test example 2 by 25 times (i.e., diluting the bird's nest peptide concentration to 0.1%) were stored at different temperatures for 4 weeks. The test results are shown in fig. 9. As can be seen from fig. 9, after the stability acceleration for 12 weeks, neither the composition of the embedded bird's nest peptide prepared in test example 2 nor the dilution prepared in test example 2 showed precipitation and turbidity, and both had almost no off-taste at room temperature, and the composition prepared in test example 2 showed slight darkening of color after 4 weeks of storage at high temperature, and slight off-taste to some extent but within an acceptable range. Therefore, the coated bird's nest peptide has better stability.

In test example 2, the particle size of the obtained composition supermolecule-coated bird's nest peptide

Distribution the particle size of the particles in the composition prepared in test example 2 was measured using a nanoparticle size potentiometer and transmission electron microscopy. Wherein FIG. 10 is a graph showing the particle size distribution of particles in the composition produced in test example 2; fig. 11 and 12 are transmission electron micrographs of the composition of test example 2 at different magnifications, respectively.

From the test results shown in FIGS. 10 to 12, the average particle diameter of the particles obtained by coating the bird's nest peptide with the supermolecule in test example 2 is 66.79nm, which is far less than 100nm.

Zeta potential test of the supramolecular encapsulated bird's nest peptide of the prepared composition in test example 2

Zeta potential and PDI values (Polydispersity Index, i.e., aggregation index) of the encapsulated bird's nest peptides were measured using a nanoparticle size potentiometer. Zeta potential is a measure of the strength of mutual repulsion or attraction between particles. The smaller the molecule or dispersed particle, the higher the absolute value of the Zeta potential (positive or negative), the more stable the system, i.e. the dissolution or dispersion can resist aggregation. The PDI value is a parameter describing the uniformity of the particle size distribution and can be calculated in connection with the particle size of the composition obtained by measuring with a nanosize potentiometer. The smaller the value of PDI, the more uniform the particle size distribution; conversely, the larger the value of PDI, the more non-uniform the particle size distribution. FIG. 13 (a) is a graph showing the Zeta potential of the composition prepared in test example 2. As is clear from the test results in FIG. 13, the Zeta potential of the composition sample coated with the bird's nest peptide prepared in test example 2 is-64.41 mV, and the composition sample has high stability. Further, the PDI value obtained by calculating the particle size of the composition for coating the bird's nest peptide prepared in combination with the test example 2 is 0.182, and the value is smaller, which further indicates that the liposome particles for coating the bird's nest peptide prepared in the test example 2 are more uniformly distributed.

In test example 2, the prepared composition was subjected to Raman transdermal test

FIG. 14 is a Raman spectrum of a sample of the composition obtained in test example 2. The Raman characteristic peak is at 835cm ^-11281cm^-11425cm^-12886cm^-1, can be clearly distinguished from the skin intrinsic peak, has higher Raman intensity, and the characteristic peak Raman characteristic peak at 1425cm ^-1 and 2886cm ^-1 is selected as the representative characteristic peak of the composition sample with the bird's nest peptide concentration of 2.5wt% for subsequent data processing and image tracking. The test results are shown in fig. 15.

As can be seen from fig. 15, the sample of test example 2 did not undergo any permeation within 0 h; the sample of test example 2 penetrated into the superficial stratum corneum and continued to penetrate into the deep stratum corneum within 2h, and the sample of test example 2 penetrated through the stratum corneum into the active epidermis but not into the dermis within 4 h.

Transdermal differences between the bird's nest peptide powder prepared in test example 1 and the supermolecule-coated bird's nest peptide composition prepared in test example 2

Reference is made to GB/T27818-2011 standard for skin absorption in vitro test methods for chemicals. The test selects the pigskin as a test model and selects the pigskin-Franz cell diffusion cell system as a test system. The bird's nest peptide powder prepared in test example 1 was reconstituted into an aqueous solution having a bird's nest peptide mass concentration of 20%, and the transdermal difference between the aqueous solution and the supermolecule-coated bird's nest peptide composition prepared in test example 2 (bird's nest peptide mass concentration of 2.5%) was compared, and the test results are shown in table 2.

Table 2 transdermal penetration of the nidus Collocaliae peptide and the coated nidus Collocaliae peptide

Sample name	Test item	Permeability/2 h	Permeability/8 h	Permeability/12 h
					Test example 1	Transdermal absorption quantification of milk pig skin	0.00051	0.00110	0.00199
Test example 2	Transdermal absorption quantification of milk pig skin	0.0076	0.0091	0.0225

From the results in Table 2, the quantitative test results of the skin percutaneous absorption of the suckling pigs show that: the permeability of the liposome-embedded bird's nest peptide composition prepared in the test example 2 is 15.2 times, 8.27 times and 11.3 times of the permeability of the bird's nest peptide aqueous solution in 2 hours, 8 hours and 12 hours, so that the bird's nest peptide wrapped by the supermolecular liposome wrapping process has good transdermal absorption capability.

Test example 3

The preparation and the encapsulation process of the bird's nest peptide are the same as those of the test example 2, but the addition amount of the bird's nest peptide is 0.1%.

Test example 4

The preparation and the encapsulation process of the bird's nest peptide are the same as those of the experimental example 2, but the adding amount of the bird's nest peptide is 10%.

Evaluation of antioxidant, photo-aging (anti-wrinkle) efficacy of the compositions prepared in test example 3 and test example 4:

The testing method comprises the following steps:

DPPH radical scavenging ability test: the compositions prepared in test example 3 and test example 4 are respectively diluted into sample solutions with the mass concentration of the bird's nest peptide shown in table 3 and the mass solubility of the required bird's nest peptide, 2mL of the sample solutions with different concentrations are respectively taken, 2mL of DPPH solution is added, the mixture is uniformly mixed, and after the mixture is placed at room temperature for 30min, the mixture is centrifuged at 5000r/min for 10min. The supernatant was taken and absorbance was measured at 517 nm.

DPPH radical scavenging was calculated according to the following formula:

Wherein,

A0: absorbance value of 2mL absolute ethanol+2 mL DPPH radical solution;

a1: absorbance value of 2mL sample solution+2 mL DPPH radical solution;

A2: absorbance value of 2mL sample solution+2 mL absolute ethanol.

ABTS free radical scavenging ability test: 7mmol/L ABTS aqueous solution and 2.45mmol/L potassium persulfate are mixed at a ratio of 1:1 (v/v) and reacted at room temperature for 12-16 h in the absence of light. Then diluted with 1mmol/L PBS to give an absorbance at 734nm of 0.70.+ -. 0.02. The compositions of the embedded bird's nest peptides prepared in test example 3 and test example 4 were diluted into sample solutions of the required bird's nest peptide mass solubility shown in table 4, 0.4mL was taken, 4mL of the diluted ABTS ⁺ -solution was added, absorbance a ₁ was measured at 734nm after reaction for 6min in the dark, absorbance a ₂ was measured with distilled water instead of ABTS ⁺ -solution as a blank group, and absorbance a ₀ was measured with distilled water instead of the sample as a control group.

ABTS ⁺ clearance was calculated as follows:

Wherein,

A ₀: absorbance value of 0.4mL ultrapure water+4 mL ABTS radical solution;

A ₁: absorbance value of 0.4mL sample solution+4 mL ABTS radical solution;

a ₂: absorbance value of 0.4mL sample solution+4 mL ultrapure water.

Evaluation of anti-photoaging (anti-wrinkle) efficacy: selecting young zebra fish with 2dpf which are normal in development, dividing the zebra fish into three groups, namely a normal control group, a model control group and a test sample group, randomly distributing the zebra fish into 6-hole cell culture plates, removing embryo culture solution from the 6-hole cell culture plates under the condition of not damaging the young fish by 15 tails per hole, adding 3mL of embryo culture solution into the normal control group and the model control group, and adding 3mL of prepared coated bird's nest peptide solution into the test sample group. Firstly, after fully and uniformly mixing, a culture plate cover plate is covered, and the culture plate is incubated for 2 hours in a dark place. Then, three irradiation times were performed with ultraviolet rays for 30 seconds at intervals of 30 minutes. Each time, the irradiation dose was 237878.54 + -0.23 J/cm ², and the total irradiation dose was 713635.62 + -0.69 J/cm ². Finally, the incubation is continued for 22 hours in a constant temperature incubator at 28+/-0.5 in a dark place, and the observation is carried out by using a biological microscope.

Test results

The DPPH free radical scavenging ability and the ABTS free radical scavenging ability test results of the sample solutions prepared in test example 3 and test example 4 and diluted to different concentrations of the bird's nest peptide are shown in tables 3 and 4, and the DPPH free radical scavenging ability of the composition coated with the bird's nest peptide is not significantly different (P is more than 0.05) in the concentration range of 100 mug/mL (0.1%) to 10000 mug/mL (10.0%), has an antioxidation effect and shows concentration dependence; the composition coated with the bird's nest peptide has no significant difference (P is more than 0.05) in the concentration range of 100 mug/mL (0.1%) to 10000 mug/mL (10.0%), has the antioxidation effect, and shows concentration dependence. TABTS free radical scavenging ability of sample solutions for dilution of the compositions for embedding bird's nest peptides prepared in test example 3 and test example 4 to different bird's nest peptide concentrations

TABLE 4 DPPH radical scavenging ability of sample solutions diluted to different concentrations of the bird's nest peptide with the compositions prepared in test example 3 and test example 4

According to the maximum tolerance concentration of the zebra fish, the concentration of the composition wrapping the bird's nest peptide of the test example 3 and the test example 4 is diluted to 100 mug/mL (based on the content of the bird's nest peptide), and as can be seen from the combination of fig. 16, the tail fin of the zebra fish (without ultraviolet irradiation) of the control group is firstly normal in development, but the zebra fish tail fin model group exposed to ultraviolet rays shows obvious malformation and shrinkage, and the result shows that the zebra fish ultraviolet induced photoaging model is successfully established. The coated bird's nest peptides of test example 3 and test example 4 were significantly improved in both the UV irradiated zebra fish tail fins with 100 g/mL intervention, but no significant difference was seen between the two. Thus, test example 3 and test example 4 showed anti-photoaging efficacy in vivo at 100 g/mL intervention as a model organism-zebra fish model result.

According to the maximum tolerance concentration of the zebra fish, the concentration of the composition wrapping the bird's nest peptide of the test example 3 and the test example 4 is diluted to 250 mug/mL (based on the content of the bird's nest peptide), and as can be seen from the combination of fig. 17, the tail fin of the zebra fish (without ultraviolet irradiation) of the control group is firstly normal in development, but the zebra fish tail fin model group exposed to ultraviolet rays shows obvious malformation and shrinkage, and the result shows that the zebra fish ultraviolet induced photoaging model is successfully established. The coated bird's nest peptides of test example 3 and test example 4 were significantly improved in both the UV irradiated zebra fish tail fins with 250 g/mL intervention, but no significant difference was seen between the two. Thus, test example 3 and test example 4 showed anti-photoaging efficacy in vivo at 250 g/mL intervention as a model organism-zebra fish model result.

From the above conclusion, the encapsulation process of test example 2 is adopted, and the supermolecular liposome containing 0.1% and 10% of bird's nest peptide by mass percent has consistent antioxidant effect and zebra fish anti-aging effect results of test example 3 and test example 4, and no significant difference. Therefore, the bird's nest peptide with the mass percent of 0.1-10% is added in the process, and the product property stability is not affected.

Test example 5

The components are identical to those of test example 2, but the pressure value in the high-pressure emulsification process is 500bar.

Test example 6

The components are identical to those of test example 2, but the pressure value in the high-pressure emulsification process is 1200bar.

Comparative example 1

The preparation and encapsulation process of the bird's nest peptide are the same as those of test example 2, but the hydrogenated lecithin is replaced by lecithin.

Comparative example 2

In accordance with the components of test example 2, a common high-speed homogenizing emulsifying machine is adopted, and no pressure value exists.

Test examples 2, 5 and 6 and comparative examples 1 to 2 were tested for encapsulation efficiency of the encapsulated bird's nest peptide

The coating rate (%) = (total bird's nest peptide content-free bird's nest peptide content)/total bird's nest peptide content in the sample. Wherein, the content of the bird's nest peptide is tested by adopting a Fu Lin Fen method. Taking test example 2 as an example, the content of the measured free bird's nest peptide is 0.94%, the content of the total bird's nest peptide is 2.7% after the wrapping material is destroyed by methanol, so that the wrapping rate of the bird's nest peptide in the sample is 65.2%, and so on. The encapsulation efficiency of the encapsulated bird's nest peptide in test examples 2, 5 and 6 and comparative examples 1 to 2 is shown in Table 5.

Table 5 test examples 2, 5, 6 and comparative examples 1 to 2 were tested for encapsulation efficiency of encapsulated bird's nest peptide

Sample name	Test item	Test results%	Test instrument
				Test example 2	Packing percentage	65.2	UV-Vis
Test example 5	Packing percentage	48.5	UV-Vis
				Test example 6	Packing percentage	65.4	UV-Vis
Comparative example 1	Packing percentage	34.2	UV-Vis
				Comparative example 2	Packing percentage	13.2	UV-Vis

From the above results, the encapsulation process of the supermolecule can successfully encapsulate the nidus Collocaliae peptide, and has higher encapsulation efficiency under the process conditions of test example 2 and test example 6, and the encapsulation efficiency of the nidus Collocaliae peptide is improved along with the increase of the homogenizing pressure within the pressure range of 500-1000 bar.

Test example 7

The composition of the supramolecular liposome coated bird's nest peptide prepared in the test example 2 is added into the essence according to the mass percentage of 12%, so as to prepare the essence with the effects of moisturizing, relieving, resisting wrinkles, repairing, tightening, whitening and nourishing.

The essence comprises the following other raw material components in percentage by mass: 85.44% deionized water, 0.02% sodium hyaluronate, 0.47% polyacrylate crosslinked polymer-6, 0.1% allantoin, 0.02% EDTA-2NA, 0.15% sodium stearoyl glutamate, 0.35% p-hydroxyacetophenone, CF (Alchemy Ingredients), 0.3% white pool seed oil, 0.5%1, 2-hexanediol, 0.05% fragrance, 0.1%SOLUBILISANT LRI (American Senxin).

Comparative example 3

The prepared essence has the same raw material components as in test example 7, but the composition of the supramolecular liposome-coated bird's nest peptide prepared in test example 2 is not added.

Comparative test example 7 and comparative example 3 facial test effects

Referring to the test method of the anti-wrinkle, tightening, moisturizing, oil control, repairing, nourishing and soothing seven effects of the T/CAB 0152-2022 cosmetic, 33 subjects 25-45 years old are selected, and the essence prepared in test example 7 and comparative example 3 is used for testing the moisturizing, soothing, anti-wrinkle, repairing, tightening, whitening and nourishing effects of the subjects after 28 days of use. The specific method is to select the face of the subject, and test example 7 and comparative example 3 are subjected to a left-right face comparison test and used for 28 days continuously. The test results are shown in tables 6 and 7, wherein:

Comparing the change before and after the use of the test sample group with the change of the difference value of the blank control group and the test sample group through the skin melanin content of the test sample group and the blank control group; and evaluating whether the test product has whitening effect.

The subject uses the sample for 14 days and before and after 28 days, the wrinkle area ratio and the skin smoothness (SEsm) of the test sample group and the blank control group are used, the change before and after the use of the test sample group and the change of the difference value between the blank control group and the test sample group are compared, and whether the test product has the anti-wrinkle effect is evaluated;

The skin moisture loss value, the skin red area a value, the skin cuticle moisture content and the skin heme content of the test sample group and the blank control group are compared, and the change before and after the use of the test sample group and the change of the difference value between the blank control group and the test sample group are compared to evaluate whether the test product has the repairing effect;

comparing the change before and after the use of the test sample group with the change of the difference value between the blank control group and the test sample group by the red skin area a value and the skin heme content of the test sample group and the blank control group, and evaluating whether the test product has a relieving effect;

Comparing the skin elasticity (R2, R5) and the skin compactness (R0, F4) of the test sample group with the skin elasticity (R5) and the skin compactness (R0, F4) of the blank control group, and comparing the change before and after the use of the test sample group with the change of the difference value between the blank control group and the test sample group to evaluate whether the test product has the compactness efficacy;

comparing the moisture content of the skin cuticle of the test sample group and the moisture content of the skin cuticle of the blank control group with the change before and after the use of the test sample group and the change of the difference value between the blank control group and the test sample group, and evaluating whether the test product has the moisturizing effect;

And comparing the change before and after the use of the test sample group with the change of the difference value between the blank group and the test sample group through the skin elasticity (R2 and R5), the skin glossiness and the skin roughness (SEr) of the test sample group and the blank group, and evaluating whether the test product has the nourishing effect.

Table 6 test example 7 and comparative example 3 results of facial tests on the essence prepared

Remarks: * P <0.05, showing a significant difference after D28 compared to D0; d0 represents the test result after 0 day, and D28 represents the test result after 28 days; # indicates the rate of change= (D28-D0)/d0100% (positive value indicates increase and negative value indicates decrease).

As is clear from the results shown in Table 3, test example 7 has moisturizing, soothing, anti-wrinkle, repairing, tightening, whitening and nourishing effects.

Table 7 statistics of results of changes in each index after day 28 of the face test of the essence prepared in test example 7 and comparative example 3

Remarks: * Indicating a significant difference between the two.

As is clear from the results of Table 7, the essence prepared in test example 7 has significantly higher moisturizing, soothing, anti-wrinkle, repairing, tightening, whitening and nourishing effects than those of comparative example 3.

In the description of the present specification, a description referring to terms "one embodiment," "some embodiments," "examples," "specific examples," or "some examples," etc., means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the present invention. In this specification, schematic representations of the above terms are not necessarily directed to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, the different embodiments or examples described in this specification and the features of the different embodiments or examples may be combined and combined by those skilled in the art without contradiction.

While embodiments of the present invention have been shown and described above, it will be understood that the above embodiments are illustrative and not to be construed as limiting the invention, and that variations, modifications, alternatives and variations may be made to the above embodiments by one of ordinary skill in the art within the scope of the invention.

Claims

1. A composition for embedding a bird's nest peptide, comprising:

2. The composition of embedding a bird's nest peptide of claim 1, further comprising: 0 to 5 weight portions of emulsifying agent, 0 to 10 weight portions of emollient and 0 to 10 weight portions of active substance.

3. The composition of embedding bird's nest peptide according to claim 2, wherein at least one of the following conditions is satisfied:

the emulsifier comprises one or more of lecithin, hydrogenated lecithin, palmitic acid, stearic acid, olive oil PEG-7 esters, cholesterol, PEG-8 caprylic/capric glycerides, phytosterol/canola glycerides, sorbitan olive oleate, polysorbate-20, laureth-23, PEG-40 stearate, steareth-21, palmitol and PEG-75 stearate and a mixture of glyceryl stearate and steareth-20, oleyl erucate, polysorbate-80, a mixture of glyceryl stearate and PEG-75 stearate, C12-16 alcohol, octyldodecanol, PEG-30 dimer hydroxystearate, cetostearyl ether-25;

The emollient comprises one or more of dimethicone, 1, 2-hexanediol, 1, 3-butanediol, 1, 2-pentanediol, caprylic/capric triglyceride, dimethiconol, isododecane, isohexadecane, squalane, half angle squalane, cetostearyl isononanoate, oleic acid capric acid, myristyl caprate, octyl dodecanol, mineral oil, ethylhexyl stearate, coco-caprylate/caprate, shea butter, rapeseed oil, polydecene, shea butter, soybean oil, olive oil, palmitic acid, stearic acid, cholesterol, phytosterol/octyldodecanol lauroyl glutamate;

The active substances comprise one or more of ceramide NP, vitronectin, retinol, retinoid, retinoic acid, palmitoyl tripeptide-1, blue copper peptide, acetyl hexapeptide-8, snake venom peptide, conopeptide, ceramide, ergothioneine, astaxanthin, asiaticoside, sodium hyaluronate, ectoin, nicotinamide, arbutin, vitamin C, tranexamic acid, glutathione, phenethyl resorcinol, salicylic acid and fruit acid.

4. A composition of embedding bird's nest peptide according to claim 2 or 3, characterized by comprising, based on 100 parts by weight of the composition of embedding bird's nest peptide:

0.1 to 10 parts by weight of bird's nest peptide, 0.5 to 8 parts by weight of hydrogenated lecithin, 10 to 40 parts by weight of polydimethylsiloxane, 0.5 to 5 parts by weight of caprylic/capric triglyceride, 20 to 60 parts by weight of glycerin, 1 to 3 parts by weight of 1, 2-hexanediol, 0.1 to 5 parts by weight of phytosterol/octyldodecanol lauroyl glutamate, 0.001 to 0.5 part by weight of ceramide NP, 0.01 to 0.5 part by weight of stearic acid, 0.01 to 0.5 part by weight of palmitic acid, 0.01 to 0.5 part by weight of cholesterol, 0 to 5 parts by weight of the emulsifier, 0 to 10 parts by weight of the emollient, 0 to 10 parts by weight of the active substance, and the balance of water.

5. The composition of claim 4, wherein the composition comprises: 0.1 to 5 parts by weight of the emulsifier, 0.1 to 10 parts by weight of the emollient, and 0.1 to 10 parts by weight of the active substance.

6. The composition of claim 1 or 5, wherein the particle size of the particles in the composition of the embedded bird's nest peptide is 50-300 nm; and/or the number of the groups of groups,

The relative molecular mass of the bird's nest peptide is not more than 3000; and/or the number of the groups of groups,

The bird's nest peptide is added in a powder form, and the preparation method of the bird's nest peptide powder comprises the following steps:

Mixing nidus Collocaliae with water, stewing, and homogenizing to obtain nidus Collocaliae pulp;

Performing enzymolysis reaction on the bird's nest pulp under the condition that the pH value is 5-9;

inactivating enzyme of the bird's nest slurry after the enzymolysis reaction;

Centrifuging the enzyme-deactivated bird's nest slurry to obtain a bird's nest peptide crude sample solution;

filtering the coarse sample solution of the bird's nest peptide by adopting an ultrafiltration membrane with the wavelength of 50-300 nm to obtain a bird's nest peptide solution;

Concentrating and drying the bird's nest peptide to obtain bird's nest peptide powder.

7. The composition of claim 6, wherein at least one of the following conditions is satisfied:

The stewing and homogenizing of the bird's nest is preceded by: cleaning the bird's nest;

Mixing the bird's nest and water according to the mass ratio of (0.1-0.5): 1, mixing and stewing, wherein the stewing time is 0.5-2 h, and the homogenizing pressure is 10-30 Mpa;

adjusting the pH value of the bird's nest slurry to 5-9 by using sodium carbonate;

Performing enzymolysis reaction on the bird's nest slurry by using alkaline protease, wherein the temperature of the enzymolysis reaction is 40-60 and the time is 1-6 h;

the temperature of the enzyme deactivation treatment is 80-100 and the time is 10-30 min;

The rotational speed of the centrifugal treatment is 10000-15000 rpm/min.

8. A method of preparing the composition of embedded bird's nest peptide of any one of claims 1 to 7, comprising:

9. The method of claim 8, wherein at least one of the following conditions is satisfied:

When preparing the solution A, the method further comprises the following steps: dissolving an emulsifier in the glycerol;

when preparing the solution C, the mixed dissolution further comprises: adding emollients and/or actives;

When the ABC mixed phase is prepared, the solution A and the solution B are uniformly mixed and then mixed with the solution C;

Emulsifying the ABC mixed phase for 1-5 times under high pressure;

The pressure for carrying out high-pressure emulsification on the ABC mixed phase is 700-1200 bar;

the speed of homogenizing the ABC mixed phase is 15000-20000 rpm/min.

10. The method according to claim 8 or 9, characterized in that at least one of the following conditions is fulfilled:

When the solution A is prepared, the dissolution is carried out under the stirring condition, the temperature of the dissolution is not lower than 70 , the stirring speed is 300-500 rpm/min, and the time is 0.5-1.5 h;

when preparing the solution B, the dissolution is carried out under heating conditions;

When the solution C is prepared, the dissolution is carried out under the stirring condition, the temperature of the dissolution is 90-180 , the stirring speed is 300-500 rpm/min, and the time is 0.5-1.5 h;

When the ABC mixed phase is prepared, firstly mixing the solution A and the solution B, stirring for 0.5-1.5 h at the rotating speed of 300-500 rpm/min, then mixing the solution A and the solution B with the solution C, and stirring for 0.5-1.5 h at the rotating speed of 300-500 rpm/min at the temperature of not lower than 50 ;

the temperature of the high-pressure emulsification and the homogenization treatment is 20-80 .

11. A skin care product comprising the composition of embedded bird's nest peptide according to any one of claims 1 to 7 and/or the composition of embedded bird's nest peptide produced by the method according to any one of claims 8 to 10.