CN117866730A - Magnetic frame device suitable for ninety-six orifice plate - Google Patents
Magnetic frame device suitable for ninety-six orifice plate Download PDFInfo
- Publication number
- CN117866730A CN117866730A CN202311304295.7A CN202311304295A CN117866730A CN 117866730 A CN117866730 A CN 117866730A CN 202311304295 A CN202311304295 A CN 202311304295A CN 117866730 A CN117866730 A CN 117866730A
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- Prior art keywords
- magnet
- ninety
- magnetic
- aluminum sheet
- magnet box
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Links
- 229910052782 aluminium Inorganic materials 0.000 claims abstract description 27
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 claims abstract description 25
- 239000011148 porous material Substances 0.000 claims abstract description 22
- 239000011810 insulating material Substances 0.000 claims abstract description 3
- 238000000926 separation method Methods 0.000 claims description 13
- 239000011324 bead Substances 0.000 claims description 9
- 239000012774 insulation material Substances 0.000 claims description 4
- 238000000034 method Methods 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 238000000746 purification Methods 0.000 description 4
- 238000007885 magnetic separation Methods 0.000 description 3
- 230000007547 defect Effects 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 239000006249 magnetic particle Substances 0.000 description 2
- 230000036544 posture Effects 0.000 description 2
- 238000001742 protein purification Methods 0.000 description 2
- 238000001179 sorption measurement Methods 0.000 description 2
- 238000007400 DNA extraction Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005342 ion exchange Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000005389 magnetism Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000011325 microbead Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000001821 nucleic acid purification Methods 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000000751 protein extraction Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
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- Physical Or Chemical Processes And Apparatus (AREA)
Abstract
The invention discloses a magnetic frame device suitable for a ninety-six pore plate, which mainly comprises a magnet box, a magnet, an aluminum sheet, a bolt, a nut and other components. The magnet comprises two rectangular strong magnets, and is horizontally arranged in a magnet placing groove at the top of the magnet box. The aluminum sheet is made of a magnetic insulating material and is horizontally placed in an aluminum sheet placing groove at the bottom of the magnet box. The bolts and the nuts are respectively two, the bolts penetrate through bolt holes symmetrically distributed on the magnet, the magnet box and the aluminum sheet from the vertical direction, and the two nuts are used for fixing the bottom end of the magnet box. The magnet box top sets up the buckle along long limit direction for fixed ninety-six orifice plate bases. In addition, a slide rail is reserved at the top of the magnet box so that the ninety-six pore plate can horizontally slide into the other end of the magnet box. And a limiting plate is arranged in the short side direction of the top of the magnet box and used for limiting the ninety-six pore plates to stay in the magnetic attraction area. The device can be used for conveniently installing and fixing the ninety-six pore plates, and has good practicability and convenience.
Description
Technical Field
The invention relates to a frame device suitable for ninety-six pore plates, in particular to a magnetic frame for magnetic bead purification, and belongs to the technical field of biological devices such as protein and nucleic acid purification.
Background
At present, the magnetic separation technology is widely applied to DNA extraction experiments, and magnetic particles can be adsorbed in the magnetic separation operation, so that the rapid separation and purification of target molecules in a mixture are realized. Compared with the conventional separation technology such as precipitation method, centrifugation method and ion exchange column method, the magnetic separation technology has the advantages of less separation equipment, high separation speed, high extraction efficiency and the like.
Ninety-six well plates are a commonly used laboratory consumable that consists of 96 well sites, each of which can hold a quantity of sample or reagent; and ninety-six well plates are typically of standardized size and location. Researchers can perform a variety of experimental procedures, such as nucleic acid or protein extraction and purification, in ninety-six well plates.
Ninety-six well plates were placed in a magnetic rack so that magnetic microbeads were adsorbed to the bottom of the wells and non-specifically bound material could be washed away. Thus, the rapid separation and purification of the target molecules can be realized. The existing ninety-six orifice plate magnetic frame device has the following defects:
1) The ninety-six orifice plate balance problem is solved, and due to the adoption of the magnetic force adsorption principle, the ninety-six orifice plate magnetic frame needs to be kept balanced in the use process so as to ensure that the magnetic beads are uniformly adsorbed at the bottom of the orifice. Incorrect manipulation or vibration may lead to uneven distribution of the beads, affecting the separation effect.
2) The problem of insufficient magnetic force, the magnetic field distribution is uneven due to the defect of the design of the magnetic frame, and the final extraction and purification effects are not ideal due to the insufficient magnetic force.
3) There is no special magnetic rack device for ninety-six pore plates, and most of the magnetic rack devices are handheld magnetic rack simple devices self-made in laboratories, such as magnetic sheets placed at specific positions in operation to realize magnetic attraction. However, this method may require more manual operations and skill, and may have some impact on experimental efficiency and results.
Disclosure of Invention
In order to solve the technical problems, the invention provides a magnetic frame device suitable for a ninety-six orifice plate so as to solve the problems.
The technical scheme adopted by the invention is as follows:
a magnetic frame device suitable for ninety-six orifice plates comprises a magnet box, a magnet, an aluminum sheet, a bolt and a nut. The magnets are two rectangular strong magnets, and the two magnets are horizontally arranged in a magnet placing groove at the top of the magnet box; the aluminum sheet is a magnetic insulation material and is horizontally placed in an aluminum sheet placing groove at the bottom of the magnet box; the bolts and the nuts are respectively provided with two bolts, the bolts sequentially penetrate through bolt holes symmetrically distributed on the magnet, the magnet box and the aluminum sheet in the vertical direction, and the bolts are respectively fixed at the bottom end of the magnet box through the two nuts.
Further, buckles are symmetrically arranged on the long side direction of the top of the magnet box and used for fixing the ninety-six pore plates.
Further, a sliding rail is reserved at the top of the magnet box, so that the ninety-six pore plate can horizontally slide into the other end of the magnet box from one end.
Further, a limiting plate is arranged in the short side direction of the top of the magnet box and used for limiting the ninety-six pore plates to stay in the magnetic attraction area.
Further, the aluminum sheet is the magnetic insulation material to place in the aluminum sheet standing groove, thereby constitute no magnetism base in magnetic force frame device bottom, avoid the magnetic field to influence magnetic force frame device and place and magnetic bead separation experimental operation.
The beneficial effects of the invention are as follows: 1. the matching of the magnet box, the magnet, the buckle, the limiting plate and the sliding rail ensures that the ninety-six pore plate can be horizontally placed on the magnet in a stable posture when the ninety-six pore plate is used for magnetic bead separation experiments, the ninety-six pore plate can not incline or vibrate in the moving process of the magnetic frame, the magnetic frame can be inverted and the ninety-six pore plate can not fall in the operation of removing supernatant waste liquid after the magnetic beads are adsorbed, the separation process is simple and effective, and the separation result is reliable; 2. by adopting the bottom suction layout, the magnetic particles can be better absorbed by penetrating through the middle pore plate; 3. the two magnets are strong magnets, the N pole and the S pole are in exchange layout, a stronger magnetic field is generated, and a better adsorption effect is ensured.
Drawings
FIG. 1 is an exploded view of the structure of the present invention;
FIG. 2 is an assembly effect diagram of the present invention;
FIG. 3 is a view of the present invention in use with a ninety-six orifice plate;
FIG. 4 is a cross-sectional view of the assembled view of FIG. 2;
FIG. 5 is a sectional view of the use of FIG. 3;
FIG. 6 is a side view of the use of FIG. 3;
FIG. 7 is an enlarged bottom view of FIG. 2;
fig. 8 is a top view of the present invention.
Wherein: the device comprises a 1-magnet box, a 2-magnet, a 3-aluminum sheet, a 4-bolt, a 5-nut, 11, 12-buckles, 13-limiting plates, 14, 15-bolt holes, 16-magnet placing grooves, 17-aluminum sheet placing grooves and 18-sliding rails.
Detailed Description
The invention will now be described in detail with reference to the drawings and the specific embodiments thereof, it being understood that the following detailed description is illustrative and explanatory only and is not restrictive of the invention.
As shown in fig. 1 to 7, a magnetic frame device suitable for a ninety-six pore plate comprises a magnet box 1, a magnet 2 aluminum sheet 3, a bolt 4 and a nut 5, wherein the magnet 2 is two rectangular strong magnets and is horizontally arranged in a magnet placing groove 16 at the top of the magnet box 1; the aluminum sheet 3 is a magnetic insulation material and is horizontally arranged in an aluminum sheet placing groove 17 at the bottom of the magnet box 1; the two bolts 4 and the two nuts 5 sequentially penetrate through bolt holes 14 and 15 symmetrically distributed on the magnet 2, the magnet box 1 and the aluminum sheet 3 from the vertical direction, and the two bolts 4 are respectively fixed at the bottom end of the magnet box 1 by the two nuts 5.
The long side direction of the top of the magnet box 1 is symmetrically provided with buckles 11 and 12 for fixing ninety-six pore plates; the shape of the buckles 11 and 12 is matched with that of the ninety-six pore plate base.
A sliding rail 18 is reserved at the top of the magnet box 1, so that a ninety-six pore plate can horizontally slide into the other end of the magnet box 1 from one end until reaching the limit plate 13; this ensures that the operation is completed in a stable and safe state when using ninety-six well plates for magnetic bead separation experiments.
A limiting plate 13 is arranged in the short side direction of the top of the magnet box 1 and used for limiting the ninety-six pore plates to stay in the magnetic attraction area; the ninety-six orifice plate is prevented from falling off when moving on the magnetic rack device slide rail 18.
In this embodiment, the bolt holes 14, 15 on the magnet box 1 are the same as the round holes on the magnet and the round holes on the aluminum sheet in position, diameter and shape, that is, the bolts 4 can pass through in vertical postures, and the magnet 2 and the aluminum sheet 3 are respectively fixed in the magnet placing groove 16 and the aluminum sheet placing groove 17 by the nuts 5.
In this embodiment, the aluminum sheet 3 is made of a magnetic insulating material and is placed in the aluminum sheet placing groove 17, that is, the bottom of the magnetic frame device is a non-magnetic base, so that the magnetic field is prevented from affecting the placement of the magnetic frame device and the experimental operation of magnetic bead separation.
The foregoing description is only intended to illustrate the technical concept and features of the present invention and should not be taken as limiting the scope of the invention, and any equivalents or modifications according to the technical scheme and concept of the present invention should be construed to be included in the scope of the present invention, which is defined by the appended claims.
Claims (5)
1. A magnetic frame device suitable for ninety-six orifice plates comprises a magnet box, a magnet, an aluminum sheet, a bolt and a nut. The magnet is characterized in that the magnets are two rectangular strong magnets, and the two magnets are horizontally arranged in a magnet placing groove at the top of the magnet box; the aluminum sheet is a magnetic insulation material and is horizontally placed in an aluminum sheet placing groove at the bottom of the magnet box; the bolts and the nuts are respectively provided with two bolts, the bolts sequentially penetrate through bolt holes symmetrically distributed on the magnet, the magnet box and the aluminum sheet in the vertical direction, and the bolts are respectively fixed at the bottom end of the magnet box through the two nuts.
2. A magnetic frame device for a ninety-six orifice plate according to claim 1, wherein the magnet box top is provided with buckles symmetrically along the longitudinal direction for fixing the ninety-six orifice plate.
3. A magnetic stand apparatus for a ninety-six aperture plate according to claim 1 wherein said magnet housing top is provided with a slide rail for allowing the ninety-six aperture plate to slide horizontally from one end into the other end of the magnet housing.
4. A magnetic frame device suitable for ninety-six pore plates according to claim 1, wherein a limiting plate is arranged on the short side direction of the top of the magnet box and is used for limiting the ninety-six pore plates to stay in a magnetic attraction area.
5. A magnetic rack device suitable for ninety-six pore plates according to claim 1, wherein the aluminum sheet is a magnetic insulating material and is placed in the aluminum sheet placing groove, so that a non-magnetic base is formed at the bottom of the magnetic rack device, and the magnetic field is prevented from affecting the placement of the magnetic rack device and the experimental operation of magnetic bead separation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311304295.7A CN117866730A (en) | 2023-10-10 | 2023-10-10 | Magnetic frame device suitable for ninety-six orifice plate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311304295.7A CN117866730A (en) | 2023-10-10 | 2023-10-10 | Magnetic frame device suitable for ninety-six orifice plate |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN117866730A true CN117866730A (en) | 2024-04-12 |
Family
ID=90588894
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202311304295.7A Pending CN117866730A (en) | 2023-10-10 | 2023-10-10 | Magnetic frame device suitable for ninety-six orifice plate |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN117866730A (en) |
-
2023
- 2023-10-10 CN CN202311304295.7A patent/CN117866730A/en active Pending
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