CN213456354U

CN213456354U - Adjustable magnetic separation device

Info

Publication number: CN213456354U
Application number: CN202022458204.3U
Authority: CN
Inventors: 李松
Original assignee: Nanjing Arix Biotechnology Co ltd
Current assignee: Nanjing Arix Biotechnology Co ltd
Priority date: 2020-10-29
Filing date: 2020-10-29
Publication date: 2021-06-15
Anticipated expiration: 2030-10-29

Abstract

The utility model provides an adjustable magnetic separation device, which comprises an upper supporting plate, a vertical plate, a lower supporting plate and a plurality of detachable permanent magnets, wherein the upper baffle plate and the lower baffle plate are arranged in parallel, the vertical baffle plate is positioned between the upper baffle plate and the lower baffle plate, the surface of the upper baffle plate is provided with a pipe hole, the surface of the lower baffle plate is provided with a lower slotted hole, and the detachable permanent magnets are detachably arranged on the surface of the vertical plate and are arranged in parallel up and down; the lower end of the vertical plate is bent inwards to form an inclined plate, and the surface of the inclined plate is also provided with a detachable permanent magnet. The device uses adjustable magnet, carries out nimble, efficient magnetic separation to the magnetic bead in bulky reaction tubes such as 15ml, 50ml to can adsorb the magnetic bead more efficiently, accomplish nimble efficient magnetic separation.

Description

Adjustable magnetic separation device

Technical Field

The utility model belongs to the technical field of a magnetic separation device for biological analysis technique, in particular to a magnetic separation device with adjustable.

Background

From the early experimental technicians, the biological enzyme is fixed on the silica and cellulose embedded iron oxide nanoparticles, and then, in the magnetic separation technology, the preparation and surface modification progress of the superparamagnetic magnetic nanoparticles (or submicron nanoparticles) is added, so that the magnetic separation device for biological analysis is once and again promoted and innovated.

Magnetic nanoparticles are the most widely used technique for biological analysis today, and have the following benefits: (1) the cost is low and the price is substantial due to repeated use; (2) the use and operation are simple, and a large amount of manpower and material resources are not required to be consumed; (3) the activity of the substance is not influenced, and the substance participating in the bioanalysis experiment is not influenced; (4) has wide application range and can be widely applied to the fields of microbiology, molecular biology, immunology and the like. The magnetic separation technology has high-efficiency and accurate biological analysis characteristics, is widely applied to various fields such as biochemistry, physiology, pharmacology, pharmacodynamics and the like, is used as a carrier of biological large and small molecules, can detect biochemical properties and indexes of a marker, is used as a stable, high-efficiency and accurate carrier, and modifies different substances on the surface of magnetic nanoparticles, so that the modified magnetic nanoparticles have more biological functions, realize various biological reactions and have the characteristic of increased versatility.

In the aspect of preparation, magnetic nanoparticles are functionally modified, and particularly in the production process, large-scale surface modifiers are added on the magnetic nanoparticles, so that the magnetic nanoparticles can be magnetically separated repeatedly for many times, the existing magnetic separation technology can only be suitable for small tubes of 1.5ml and 2ml to magnetically separate a small-volume reaction system, and most of magnetic separation devices used in the existing large-volume reaction system in the market are fixed devices, so that the flexibility and the efficiency of magnetic separation of the large-volume reaction system are greatly limited.

SUMMERY OF THE UTILITY MODEL

Utility model purpose: the defect of current magnetic separation technique is single, fixed point magnetic separation, and this kind of magnetic separation technique's flexibility ratio is low, inefficiency, no multiple biological reaction to only have single functional, the magnet that current magnetic separation device used separates the magnetic bead from single, fixed direction, consequently limits widely to a certain extent, has reduced magnetic separation's multifunctionality and efficiency, to above-mentioned technical problem, the utility model provides an adjustable magnetic separation device, the device use adjustable magnet, carry out nimble, efficient magnetic separation to bulky reaction systems such as 15ml, 50ml to more can adsorb the magnetic bead efficiently, accomplish nimble efficient magnetic separation.

The technical scheme is as follows: the utility model provides an adjustable magnetic separation device, which comprises an upper supporting plate, a vertical plate, a lower supporting plate and a plurality of detachable permanent magnets, wherein the upper supporting plate and the lower supporting plate are arranged in parallel, the vertical plate is positioned between the upper supporting plate and the lower supporting plate, the surface of the upper supporting plate is provided with a pipe hole, the surface of the lower supporting plate is provided with a lower slotted hole, and the detachable permanent magnets are detachably arranged on the surface of the vertical plate and are arranged in parallel up and down; the lower end of the vertical plate is bent inwards to form an inclined plate, and the surface of the inclined plate is also provided with a detachable permanent magnet.

As a preferable scheme:

the pore diameter of the pore is larger than that of the lower slotted hole. Each micropore reaction tube can be tightly clamped on the device, so that a perfect fixing effect is achieved. Is suitable for large-volume reaction systems of 15ml, 50ml and the like, and fills the blank of the large-volume reaction systems in the market.

The number of the pipe holes and the number of the lower slotted holes are one, and the pipe holes and the lower slotted holes are all arranged on one side of a vertical extending plane of the vertical plate.

Or the number of the pipe holes and the number of the lower slotted holes are two and are symmetrically arranged on two sides of the vertical extending plane of the vertical plate.

The quantity of permanent magnet is 4 or 8, and the riser openly and the reverse side all can set up 4 permanent magnets promptly, also can set up 4 permanent magnets simultaneously. When the user uses, the quantity of alternative adjustment permanent magnet carries out nimble ground magnetism separation adjustment to the reaction system, when using single permanent magnet, can carry out more nimble, pinpointing to the position of reaction liquid volume and magnetism separation in a flexible way, when using a plurality of permanent magnets, can realize the quick magnetism separation purpose to the reaction system, through the number of adjustment permanent magnet, can reach the purpose of multiple magnetism separation, if: rapid separation, precise separation, and the like; meanwhile, various experimental purposes such as washing, concentration and the like can be achieved.

The included angle between the inclined plate and the vertical direction is 30-60 degrees, and the design can ensure that the detachable permanent magnet on the surface of the inclined plate is matched with the bottom bending part of the micropore reaction tube and is in close contact with the bottom bending part, so that the separation efficiency and the separation precision are improved.

Has the advantages that: compared with the prior art, the utility model discloses incomplete, not thorough to the magnetic separation, provide a device that can solve this type of defect, can transfer formula magnetic separation device. The device uses an adjustable permanent magnet to carry out flexible and high-efficiency magnetic separation on large-volume reaction systems such as 15ml and 50ml, so that magnetic beads can be adsorbed more efficiently, and flexible and high-efficiency magnetic separation is completed; in addition, the adjustable permanent magnet adsorption device different from the existing magnetic separation device is used, flexible and efficient magnetic separation is realized to a great extent, a user can realize various magnetic separation positions, and by changing the position of the permanent magnet, the purposes of magnetic separation at various angles in a large-volume reaction system, various bioanalysis experiments such as concentration and washing can be carried out, and various experiment purposes are achieved; in addition, different substances are modified on the magnetic nano particles, so that more abundant and diversified bioanalysis experiments can be completed, and the multifunctional magnetic nano particle is multifunctional; and simultaneously, the utility model discloses reached with low costs, operation convenient to use, equipment easy, effectual advantage and progress under these basic premises, greatly improved the efficiency of magnetic separation.

Drawings

Fig. 1 is a schematic structural diagram of an adjustable magnetic separation apparatus according to embodiment 1.

FIG. 2 is a schematic side view of the adjustable magnetic separation apparatus of embodiment 1 in use;

fig. 3 is a schematic structural diagram of an adjustable magnetic separation apparatus according to embodiment 2.

Detailed Description

The present invention will be further explained with reference to the accompanying drawings.

Example 1

An adjustable magnetic separation device is shown in figure 1 and comprises an upper supporting plate 1, a vertical plate 2, a lower supporting plate 3 and a plurality of detachable permanent magnets 4, wherein the upper supporting plate 1 and the lower supporting plate 3 are arranged in parallel, the vertical plate 2 is positioned between the upper supporting plate 1 and the lower supporting plate 3, pipe holes 11 are formed in the surface of the upper supporting plate 1, lower groove holes 31 are formed in the surface of the lower supporting plate 3, and the detachable permanent magnets 4 are detachably mounted on the surface of the vertical plate 2 and are arranged in parallel up and down; the lower end of the vertical plate 2 is bent inwards to form an inclined plate 21, and the surface of the inclined plate 21 is also provided with a detachable permanent magnet 4.

The hole diameter of the pipe hole 11 is larger than that of the lower slot hole 31. Each micropore reaction tube can be tightly clamped on the device, so that a perfect fixing effect is achieved. Is suitable for large-volume reaction systems of 15ml, 50ml and the like, and fills the blank of the large-volume reaction systems in the market.

The number of the pipe holes 11 and the number of the lower slotted holes 31 are one, and the pipe holes and the lower slotted holes are arranged on one side of a vertical extending plane of the vertical plate 2.

The number of permanent magnets 4 is 4. When the user uses, the position of alternative adjustment permanent magnet 4 carries out nimble ground magnetic separation adjustment to the reaction system, can reach the purpose of multiple magnetic separation, if: rapid separation, precise separation, and the like; meanwhile, various experimental purposes such as washing, concentration and the like can be achieved.

The included angle between the inclined plate 21 and the vertical direction is 45 degrees, and the design can ensure that the detachable permanent magnet on the surface of the inclined plate is matched with the bottom bending part of the micropore reaction tube, so that the inclined plate is in close contact with the bottom bending part of the micropore reaction tube, and the separation efficiency and the separation precision are improved.

As shown in fig. 2, in the embodiment, the hole groove of the magnetic separation device can accommodate reaction system test tubes with large volumes such as 15ml, 50ml, and the like, and each partition plate is provided with a hole, so that when the microporous reaction tube 5 is accommodated, each microporous reaction tube can be tightly connected with the device and the permanent magnet, which is beneficial to improving the magnetic separation efficiency and the separation degree; the body uses the light-duty material preparation of environmental protection resin, satisfies circulation, environmental protection, healthy theory, and is little to environmental pollution harm, and the size of a dimension that is equipped with 4 adjustable magnet altogether is 1 ~ 10cm, and the diameter in aperture is 10 ~ 30cm, the utility model discloses the size of a dimension is long: 10-35 cm, width: 10-35 cm, height: 20 ~ 120cm, the size and dimension can satisfy 15ml, bulky reaction systems such as 50ml carry out high-efficiently, it is nimble, magnetic separation purpose simultaneously, four permanent magnets can carry out the magnetic separation of multiunit combination diversified to the reaction liquid from a plurality of position and 45 oblique angles of micropore pipe lateral wall respectively, slotted hole 31 can fix micropore reaction pipe 5 steadily down, both guarantee the stability of micropore reaction pipe on the device, the dismantled permanent magnet of device can be more nimble, adsorb the micropore magnetic bead high-efficiently, reach more multi-functionally at bioanalysis, high efficiency, nimble magnetic separation purpose.

Example 2

Basically the same as example 1, except that:

as shown in fig. 3, the number of the pipe holes 11 and the number of the lower slot holes 31 are two, and the two holes are symmetrically arranged on two sides of the vertical extension plane of the vertical plate 2, and the number of the permanent magnets 5 is 4 or 8. The device can carry out the magnetic separation of two micropore reaction tubes simultaneously, and four magnets of every group can realize that multiunit closes diversely from the different angles and the 45 oblique angles of micropore pipe lateral wall respectively and carry out the magnetic separation.

On the basis of the above embodiment 2, the device of the utility model can also be connected in parallel, and simultaneously satisfies the magnetic separation of a plurality of micropore reaction tubes.

Claims

1. An adjustable magnetic separation device is characterized by comprising an upper supporting plate (1), a vertical plate (2), a lower supporting plate (3) and a plurality of detachable permanent magnets (4), wherein the upper supporting plate (1) and the lower supporting plate (3) are arranged in parallel, the vertical plate (2) is positioned between the upper supporting plate (1) and the lower supporting plate (3), pipe holes (11) are formed in the surface of the upper supporting plate (1), lower slotted holes (31) are formed in the surface of the lower supporting plate (3), and the detachable permanent magnets (4) are detachably mounted on the surface of the vertical plate (2) and arranged in parallel up and down; the lower end of the vertical plate (2) is bent inwards to form an inclined plate (21), and the surface of the inclined plate (21) is also provided with a detachable permanent magnet (4).

2. Adjustable magnetic separation device according to claim 1, characterized in that the aperture of the pipe hole (11) is larger than the aperture of the lower slotted hole (31).

3. The adjustable magnetic separator according to claim 1, wherein the number of said pipe holes (11) and said lower slotted holes (31) is one, and they are arranged on one side of the vertical extension plane of the vertical plate (2).

4. The adjustable magnetic separator according to claim 1, wherein the number of said pipe holes (11) and lower slots (31) is two, and they are symmetrically arranged on both sides of the vertical extension plane of the vertical plate (2).

5. Adjustable magnetic separation device according to claim 1, characterized in that the number of permanent magnets (4) is 4 or 8.

6. The adjustable magnetic separation device according to claim 1, wherein the angle between the inclined plate (21) and the vertical direction is 30-60 °.