CN117844632A - Detection device for detecting gastroesophageal reflux of human body and preparation method of quality control product of detection device - Google Patents
Detection device for detecting gastroesophageal reflux of human body and preparation method of quality control product of detection device Download PDFInfo
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- CN117844632A CN117844632A CN202311815188.0A CN202311815188A CN117844632A CN 117844632 A CN117844632 A CN 117844632A CN 202311815188 A CN202311815188 A CN 202311815188A CN 117844632 A CN117844632 A CN 117844632A
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- 238000001514 detection method Methods 0.000 title claims abstract description 90
- 208000021302 gastroesophageal reflux disease Diseases 0.000 title claims abstract description 23
- 238000003908 quality control method Methods 0.000 title claims abstract description 20
- 238000002360 preparation method Methods 0.000 title abstract description 11
- 108091005804 Peptidases Proteins 0.000 claims abstract description 51
- 239000004365 Protease Substances 0.000 claims abstract description 51
- 239000007788 liquid Substances 0.000 claims abstract description 19
- 238000009990 desizing Methods 0.000 claims abstract description 4
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims abstract 19
- 239000003153 chemical reaction reagent Substances 0.000 claims description 49
- 238000001125 extrusion Methods 0.000 claims description 40
- 102000057297 Pepsin A Human genes 0.000 claims description 33
- 108090000284 Pepsin A Proteins 0.000 claims description 33
- 229940111202 pepsin Drugs 0.000 claims description 33
- 210000003296 saliva Anatomy 0.000 claims description 22
- 238000012360 testing method Methods 0.000 claims description 22
- 239000000725 suspension Substances 0.000 claims description 12
- 238000000034 method Methods 0.000 claims description 10
- 239000000243 solution Substances 0.000 claims description 10
- 238000004108 freeze drying Methods 0.000 claims description 6
- 238000000926 separation method Methods 0.000 claims description 6
- 239000011521 glass Substances 0.000 claims description 5
- 210000000214 mouth Anatomy 0.000 claims description 4
- 102000001554 Hemoglobins Human genes 0.000 claims description 3
- 108010054147 Hemoglobins Proteins 0.000 claims description 3
- 239000007853 buffer solution Substances 0.000 claims description 3
- 238000007710 freezing Methods 0.000 claims description 3
- 230000008014 freezing Effects 0.000 claims description 3
- 239000002504 physiological saline solution Substances 0.000 claims description 3
- 238000004321 preservation Methods 0.000 claims description 3
- 239000003761 preservation solution Substances 0.000 claims description 3
- 239000003223 protective agent Substances 0.000 claims description 3
- 238000012216 screening Methods 0.000 claims description 3
- 239000003381 stabilizer Substances 0.000 claims description 3
- 239000004094 surface-active agent Substances 0.000 claims description 3
- 102000035195 Peptidases Human genes 0.000 description 32
- 230000033001 locomotion Effects 0.000 description 10
- 230000001681 protective effect Effects 0.000 description 9
- 230000000694 effects Effects 0.000 description 6
- 238000003149 assay kit Methods 0.000 description 5
- 210000004051 gastric juice Anatomy 0.000 description 5
- 208000024891 symptom Diseases 0.000 description 5
- 238000010586 diagram Methods 0.000 description 4
- 210000003238 esophagus Anatomy 0.000 description 4
- 238000010992 reflux Methods 0.000 description 3
- 238000003556 assay Methods 0.000 description 2
- 230000002496 gastric effect Effects 0.000 description 2
- 239000011435 rock Substances 0.000 description 2
- 210000002784 stomach Anatomy 0.000 description 2
- 108010033276 Peptide Fragments Proteins 0.000 description 1
- 102000007079 Peptide Fragments Human genes 0.000 description 1
- 206010000059 abdominal discomfort Diseases 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 108091007735 digestive proteases Proteins 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 210000001156 gastric mucosa Anatomy 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000004877 mucosa Anatomy 0.000 description 1
- 230000010355 oscillation Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses a detection device for detecting gastroesophageal reflux of a human body and a preparation method of quality control products thereof, and particularly relates to the technical field of biological detection. The invention also provides a preparation method of the quality control product, which comprises the steps of centrifuging and desizing a sample, preparing a protease sample, preparing layering liquid, layering protease, preparing the quality control product, and preparing the protease quality control product. The invention can shake and drop a plurality of samples simultaneously, lighten the workload of medical staff and improve the detection efficiency.
Description
Technical Field
The invention relates to the technical field of biological detection, in particular to a detection device for detecting gastroesophageal reflux of a human body and a preparation method of a quality control product thereof.
Background
Pepsin is a digestive protease secreted by the gastric mucosa master cells in the stomach and able to break down the proteins in the food into small peptide fragments;
in clinical examination, if a patient is in a stomach discomfort state, saliva is required to be collected and pepsin detection is required to be carried out on the patient, the saliva pepsin detection is a method for evaluating gastric reflux in esophagus by detecting the pepsin level in saliva, pepsin normally exists in gastric juice and not in saliva, and when esophageal mucosa is damaged and gastroesophageal reflux occurs, the pepsin in gastric juice enters the saliva, so that the pepsin level in saliva can be detected to indirectly evaluate the condition of gastric reflux in esophagus;
when pepsin in saliva of a patient is detected, the existing conventional and most effective mode is that saliva of the patient is sampled and collected, saliva in a saliva extract paper test tube is selected after centrifugal treatment and is dripped onto a detection card, after a period of time, two bars appear on the detection card, so that reflux of gastric juice in esophagus of the patient is indicated, and if one bar of resistance appears, the patient is indicated that the gastric juice in esophagus is not refluxed.
The invention patent of patent application number CN202310357636.0 discloses a pepsin assay kit and an assay method, wherein the pepsin assay kit comprises a kit, an EP (EP) reagent tube and a detection card. The structure can realize the stable and safe storage of the reagent tube, is favorable for continuous or interval carrying out of the detection test, and improves the practicability. However, the detection of the sample in this structure requires manual operation by medical staff, and the workload of medical staff is not increased when a large number of samples are detected, and the sample is inevitably polluted in the manual operation process, so that the detection structure is affected.
The invention patent of patent application number CN202311240796.3 discloses a pepsin assay kit and application thereof, and the pepsin assay kit comprises a base, a placing box, a first test tube, a second test tube, an assay device and a shaking device, wherein the pepsin in gastric juice is assayed in a mode of replacing traditional manual shaking and constant temperature box internal test by means of mechanical shaking and constant temperature water bath, and the pepsin assay kit is convenient to observe and simple to operate. However, it is difficult to shake and detect a large number of samples at the same time, and the detection efficiency is low.
Aiming at the situation, the invention provides a detection device for detecting gastroesophageal reflux of a human body and a preparation method of a quality control product thereof.
Disclosure of Invention
In order to overcome the above-mentioned drawbacks of the prior art, the present invention provides a detection device for detecting gastroesophageal reflux of a human body and a preparation method of a quality control product thereof, so as to solve the problems set forth in the above-mentioned background art.
In order to achieve the above purpose, the present invention provides the following technical solutions: the utility model provides a detect detection device of human gastroesophageal reflux, includes the detection case, the standing groove has been seted up to the inner chamber bottom of detection case, and a plurality of detection cards have been placed to the inside of standing groove, the inside of detection case is located and all is provided with the reagent pipe directly over the detection card that corresponds.
In order to evenly rock a plurality of samples simultaneously, improve detection effect and improve medical personnel's detection efficiency, preferably, the inner chamber top of detection case is provided with the structure of rocking, the structure of rocking includes the connecting rod, the connecting rod is installed at the inner chamber top of detection case, the mounting groove has all been seted up to two inner walls at detection case inner chamber top, and the inside of one of them mounting groove is provided with the lead screw, and the inside of another mounting groove is provided with the slide bar, the outside of lead screw and slide bar is provided with slider and thread block respectively, the rear side of detection case is provided with the motor, the motor output is connected with the inside lead screw coaxial transmission of one of them mounting groove, thread block and slider are threaded connection and sliding connection respectively with the connected mode of lead screw and slide bar, thread block and slider are in the mounting groove inside that corresponds, and fixed mounting is in the both ends of connecting rod respectively, the bottom of connecting rod is provided with a plurality of spinal branchs pole, and the bottom of a plurality of spinal branch pole all is provided with the ball, the inside rotation of ball is provided with the universal ball, the bottom of universal ball is provided with the bracing piece.
In order to drop into the inside sample of reagent pipe and detect card department and detect, reduce the pollution problem that manual intervention brought, the improvement detection precision, preferably, the top of reagent pipe is provided with the extrusion structure, the extrusion structure includes the positioning disk, positioning disk fixed mounting is in the bottom of bracing piece, the central part of positioning disk bottom is provided with the extrusion pole, the bottom of extrusion pole is provided with the extrusion stopper, the positioning disk bottom is located the both sides of extrusion pole and all is provided with electric putter, and electric putter's output is fixed to be provided with the supporting disk, the inside of supporting disk is provided with the pipe cap, the pipe cap passes through the top of screw-thread mounting at the reagent pipe, the extrusion stopper is installed in the inside of pipe cap, the bottom of extrusion pole extends to the inside of pipe cap, and with extrusion stopper fixed connection, the bottom of reagent pipe is provided with the drip pipe, the drip pipe is with the inside detection card position one-to-one of standing groove, and the outside of drip pipe is provided with the solenoid valve.
In order to avoid pollution caused by the sample in the detection box and to facilitate the observation of the condition in the detection box by the staff, preferably, the surface of the detection box is movably provided with a box door, and the surface of the box door is provided with a glass observation window.
In order to protect the detection card inside the placing groove, the detection card is prevented from being polluted, preferably, a protective cover is arranged inside the placing groove and is in sliding connection with the placing groove, and a pull groove is formed in the surface of the protective cover.
The invention also provides a preparation method of the quality control product, which is matched with the detection device for detecting gastroesophageal reflux of a human body, and comprises the following steps:
step one, collecting saliva pepsin samples of the oral cavity of a patient, and centrifuging and desizing the saliva pepsin samples to obtain pepsin;
step two, adding protease preservation solution into pepsin to prepare a protease sample;
step three, preparing protease separation liquids with different densities, wherein the density range is 1.083-1.257g/ml, and sequentially adding the protease separation liquids into a centrifuge tube according to the density from large to small to obtain layering liquid;
slowly adding the protease sample into the layering liquid, and centrifuging to obtain layered protease, wherein protease matched with the protease is dispersed in the protease separating liquid with each density;
step five, split charging the layered protease into different test tubes, adding physiological saline into each test tube to obtain protease suspension, adjusting the hemoglobin concentration of the protease suspension to 125g/L, and screening out protease suspension with HbA1c value of 3-5%, thus obtaining quality control product;
step six, adding a buffer solution containing a surfactant into a protease suspension with HbA1c value of 3-5% to obtain a solution with HbA1c value of 3-5% and a protease film dispersed in the solution, removing the protease film through centrifugal treatment, adding at least one of a freeze-drying protective agent or a stabilizer into the solution with HbA1c value of 3-5%, and carrying out freeze-drying or liquid freezing preservation treatment to obtain the pepsin quality control product.
The invention has the technical effects and advantages that:
1. through setting up and rocking the structure, the inside lead screw of motor drive one of them mounting groove rotates for outside screw thread piece carries out reciprocating motion, and the slider is in the outside slip guide of slide bar down, make the connecting rod can realize reciprocating motion, a plurality of branches of connecting rod drive bottom carry out reciprocating motion this moment, add the weight of reagent intraductal portion itself, make the universal ball rotate in the inside of ball ring, make the inside sample of reagent intraductal portion obtain rocking because of inertial reason in reciprocating motion's in-process, can carry out the operation of rocking to a plurality of samples simultaneously, and alleviate the burden that medical personnel manually rocked, and improve detection efficiency;
2. through setting up the extrusion structure, the electric putter operation between positioning disk and supporting disk, upwards promote the outside supporting disk of tube cap, the supporting disk is in the in-process that upwards extends for the tube cap drives whole reagent pipe and upwards extends, at this moment, the inside extrusion stopper of extrusion stopper inside the tube cap of the central point department of positioning disk drive extends towards the inside of reagent pipe, the extrusion stopper is inside after downwards extending at the reagent pipe, can discharge the inside sample of reagent pipe through the drip pipe, and drip on detecting the card, realize the automatic detection of dripping into of sample;
3. the quality control product prepared by the invention can well evaluate pepsin of a patient, correct and verify the accuracy of a detection result, and has good effects in clinical detection and supervision of in-vitro stomach diagnostic reagents.
Drawings
Fig. 1 is a schematic diagram of the overall structure of the present invention.
FIG. 2 is a schematic diagram of the internal structure of the detection box according to the present invention.
Fig. 3 is a schematic view of the structure of the protective cover of the present invention when opened.
FIG. 4 is a schematic diagram of the back structure of the detection box of the present invention.
Fig. 5 is a perspective view of a wobble structure according to the present invention.
FIG. 6 is a schematic diagram showing the structure of the reagent vessel and the cap of the present invention when they are detached.
Fig. 7 is an enlarged schematic view of fig. 5 a according to the present invention.
Fig. 8 is a flow chart of the quality control product preparation according to the present invention.
The reference numerals are: 1. a detection box; 2. a placement groove; 3. a detection card; 4. a reagent tube; 5. a connecting rod; 6. a screw rod; 7. a slide bar; 8. a slide block; 9. a screw block; 10. a motor; 11. a support rod; 12. a ball ring; 13. a universal ball; 14. a support rod; 15. a positioning plate; 16. an extrusion rod; 17. extruding the plug; 18. an electric push rod; 19. a support plate; 20. a tube cap; 21. a door; 22. a glass viewing window; 23. a protective cover; 24. drawing a groove; 25. a drip tube; 26. a solenoid valve.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
The detection device for detecting gastroesophageal reflux of a human body comprises a detection box 1, wherein a placement groove 2 is formed in the bottom of an inner cavity of the detection box 1, a plurality of detection cards 3 are placed in the placement groove 2, and reagent tubes 4 are arranged right above the corresponding detection cards 3 in the detection box 1.
Specifically, the structure can drop saliva of a patient in the reagent tube 4 into the drop hole of the detection card 3, the pepsin in the saliva is detected through the detection card 3, if two bars appear on the detection card 3, the patient is indicated to have gastroesophageal reflux symptoms, and if one bar appears on the detection card 3, the patient is indicated to have no such symptoms, and the symptom condition of the patient is detected by detecting the pepsin in the saliva of the patient.
In this embodiment, as shown in fig. 2, 3, 4, 5, 6, and 7, the top of the inner cavity of the detection box 1 is provided with a shaking structure, the shaking structure includes a connecting rod 5, the connecting rod 5 is installed at the top of the inner cavity of the detection box 1, two inner walls of the top of the inner cavity of the detection box 1 are all provided with mounting grooves, one of the mounting grooves is internally provided with a screw rod 6, the other mounting groove is internally provided with a slide bar 7, the outside of the screw rod 6 and the slide bar 7 is respectively provided with a sliding block 8 and a thread block 9, the rear side of the detection box 1 is provided with a motor 10, the output end of the motor 10 is coaxially connected with the screw rod 6 in one of the mounting grooves, the thread block 9 and the sliding block 8 are respectively in threaded connection and sliding connection, the thread block 9 and the sliding block 8 are located in the corresponding mounting grooves, and are respectively fixedly installed at two ends of the connecting rod 5, the bottom of the connecting rod 5 is provided with a plurality of struts 11, and the bottom ends of the struts 11 are respectively provided with a ball ring 12, the ball ring 12 is rotatably provided with a universal ball 13, and the bottom of the universal ball 13 is provided with a supporting rod 14.
Specifically, this structure can play the effect of automatic rocking to the inside sample of reagent pipe 4, specifically, install the test tube that is equipped with patient saliva on cap 20 through the screw thread, because the reagent pipe 4 itself that is equipped with saliva contains certain weight, when rocking, can utilize motor 10 to drive the inside lead screw 6 of one of them mounting groove and rotate, make outside screw thread piece 9 carry out reciprocating motion, at this moment, slider 8 is in slide bar 7's outside slip guide downwards, make connecting rod 5 can realize reciprocating motion, a plurality of branches 11 of bottom are driven to the connecting rod 5 at this moment carry out reciprocating motion, add the inside weight of reagent pipe 4 itself, make universal ball 13 rotate in the inside of ball ring 12, make reagent pipe 4 in reciprocating motion's in-process, because inertial cause, lead to the inside sample of reagent pipe 4 to rock, carry out the processing of rocking to the inside sample of reagent pipe 4, can improve the detection effect, this structure can operate a plurality of samples simultaneously and alleviate the burden that the manual personnel rocked, and improve detection efficiency.
In this embodiment, as shown in fig. 2, 3, 5, 6 and 7, the top of the reagent tube 4 is provided with an extrusion structure, the extrusion structure includes a positioning disk 15, the bottom of the supporting rod 14 is fixedly installed on the positioning disk 15, the central part of the bottom of the positioning disk 15 is provided with an extrusion rod 16, the bottom of the extrusion rod 16 is provided with an extrusion plug 17, the bottom of the positioning disk 15 is located at two sides of the extrusion rod 16 and is provided with an electric push rod 18, the output end of the electric push rod 18 is fixedly provided with a supporting disk 19, the inside of the supporting disk 19 is provided with a tube cap 20, the tube cap 20 is installed at the top end of the reagent tube 4 through threads, the extrusion plug 17 is installed in the tube cap 20, the bottom of the extrusion rod 16 extends to the inside of the tube cap 20 and is fixedly connected with the extrusion plug 17, the bottom of the reagent tube 4 is provided with a drip tube 25, the drip tube 25 corresponds to the positions of the detection cards 3 in the inside of the placing groove 2 one by one, and the outside of the drip tube 25 is provided with an electromagnetic valve 26.
Specifically, this structure can detect on detecting card 3 to the inside sample automatic drip of reagent pipe 4, avoid medical and other manual operations to bring the work burden, and reduce the manual intervention and cause the pollution to the sample, thereby lead to the detection precision to cause the influence, specifically, the inside sample of reagent pipe 4 is after rocking, and under the state of stopping, open solenoid valve 26, be in the electric putter 18 operation between positioning disk 15 and the supporting disk 19, upwards promote the outside supporting disk 19 of cap 20, the in-process of upwards extending supporting disk 19, make cap 20 drive whole reagent pipe 4 upwards extend, at this moment, the inside extrusion plug 17 of cap 20 is driven to the inside extension of reagent pipe 4 to the extrusion plug 17 in the central part department of positioning disk 15 bottom, after the inside downextension of reagent pipe 4, can discharge the inside sample of reagent pipe 4 through drip pipe 25, and drip on detecting card 3, realize the automated inspection to the sample.
In this embodiment, as shown in fig. 1 and 4, the surface of the detection box 1 is movably provided with a box door 21, and the surface of the box door 21 is provided with a glass observation window 22, so that the box door 21 can be closed, the sample inside the detection box 1 is prevented from being polluted, and the glass observation window 22 is convenient for a worker to observe the condition inside the detection box 1.
In this embodiment, as shown in fig. 2 and 3, the protective cover 23 is provided inside the placement groove 2, the protective cover 23 is slidably connected inside the placement groove 2, and the pull groove 24 is provided on the surface of the protective cover 23, so that the detection card 3 inside the placement groove 2 can be protected, the detection card 3 is prevented from being polluted, and meanwhile, the protective cover 23 is conveniently opened through the pull groove 24, so that the detection card 3 is used.
The working principle of the invention is as follows:
the structure of the invention is particularly used in the following steps:
firstly, placing a sample collected in the oral cavity of a patient in the reagent tube 4, mounting the reagent tube 4 on the bottom of the tube cap 20 through threads, and performing oscillation and shaking treatment on the sample in the reagent tube 4;
the motor 10 is utilized to drive the screw rod 6 in one of the installation grooves to rotate, so that the external screw block 9 reciprocates, at the moment, the sliding block 8 slides downwards outside the sliding rod 7, so that the connecting rod 5 can realize reciprocating movement, at the moment, the connecting rod 5 drives the plurality of supporting rods 11 at the bottom to reciprocate, and the weight of the inside of the reagent tube 4 is added, so that the universal ball 13 rotates in the ball ring 12, so that the sample in the reagent tube 4 is shaken due to inertia in the reciprocating movement process, the sample in the reagent tube 4 is shaken, the detection effect can be improved, and the workload of medical staff is reduced;
when the sample in the reagent tube 4 is shaken and is in a stop state, the electromagnetic valve 26 is opened, the protective cover 23 is opened, a plurality of detection cards 3 in the placing groove 2 are exposed, the electric push rod 18 arranged between the positioning disc 15 and the supporting disc 19 operates to lift the supporting disc 19 outside the tube cap 20 upwards, the tube cap 20 drives the whole reagent tube 4 to extend upwards in the upward extending process of the supporting disc 19, at the moment, the extrusion rod 16 at the central part of the bottom of the positioning disc 15 drives the extrusion plug 17 in the tube cap 20 to extend into the reagent tube 4, and after the extrusion plug 17 extends downwards in the reagent tube 4, the sample in the reagent tube 4 can be discharged through the drip tube 25 and drip on the detection cards 3, so that automatic drip detection of the sample is realized;
after the sample is dropped on the test card 3, waiting for a period of time, if two bars appear on the test card 3, the patient is indicated to have gastroesophageal reflux disease, and if one bar appears on the test card 3, the patient is indicated to have no such symptom, and the symptom condition of the patient is detected by detecting pepsin in saliva of the patient.
As shown in fig. 8, the invention further provides a preparation method of a quality control product, which is matched with the detection device for detecting gastroesophageal reflux of a human body, and the preparation method comprises the following steps:
step one, collecting saliva pepsin samples of the oral cavity of a patient, and centrifuging and desizing the saliva pepsin samples to obtain pepsin;
step two, adding protease preservation solution into pepsin to prepare a protease sample;
step three, preparing protease separation liquids with different densities, wherein the density range is 1.083-1.257g/ml, and sequentially adding the protease separation liquids into a centrifuge tube according to the density from large to small to obtain layering liquid;
slowly adding the protease sample into the layering liquid, and centrifuging to obtain layered protease, wherein protease matched with the protease is dispersed in the protease separating liquid with each density;
step five, split charging the layered protease into different test tubes, adding physiological saline into each test tube to obtain protease suspension, adjusting the hemoglobin concentration of the protease suspension to 125g/L, and screening out protease suspension with HbA1c value of 3-5%, thus obtaining quality control product;
step six, adding a buffer solution containing a surfactant into a protease suspension with HbA1c value of 3-5% to obtain a solution with HbA1c value of 3-5% and a protease film dispersed in the solution, removing the protease film through centrifugal treatment, adding at least one of a freeze-drying protective agent or a stabilizer into the solution with HbA1c value of 3-5%, and carrying out freeze-drying or liquid freezing preservation treatment to obtain the pepsin quality control product.
The foregoing description of the preferred embodiments of the invention is not intended to limit the invention to the precise form disclosed, and any such modifications, equivalents, and alternatives falling within the spirit and principles of the invention are intended to be included within the scope of the invention.
Claims (10)
1. The utility model provides a detect detection device of human gastroesophageal reflux, includes detection case (1), its characterized in that: a placing groove (2) is formed in the bottom of an inner cavity of the detection box (1), a plurality of detection cards (3) are placed in the placing groove (2), and reagent tubes (4) are arranged right above the corresponding detection cards (3) in the detection box (1);
a shaking structure is arranged at the top of the inner cavity of the detection box (1);
the top of the reagent tube (4) is provided with an extrusion structure.
2. A test device for detecting gastroesophageal reflux in a human according to claim 1, wherein: the shaking structure comprises a connecting rod (5), the connecting rod (5) is arranged at the top of an inner cavity of the detection box (1), mounting grooves are formed in two inner walls of the top of the inner cavity of the detection box (1), a screw rod (6) is arranged in one mounting groove, a sliding rod (7) is arranged in the other mounting groove, a sliding block (8) and a thread block (9) are respectively arranged outside the sliding rod (7) and the screw rod (6), and a motor (10) is arranged at the rear side of the detection box (1).
3. A test device for detecting gastroesophageal reflux in a human according to claim 2, wherein: the output end of the motor (10) is coaxially connected with the screw rod (6) in one of the mounting grooves, the threaded blocks (9) and the sliding blocks (8) are in threaded connection and sliding connection with the screw rod (6) and the sliding rod (7) respectively, and the threaded blocks (9) and the sliding blocks (8) are located in the corresponding mounting grooves and are fixedly mounted at two ends of the connecting rod (5) respectively.
4. A test device for detecting gastroesophageal reflux in a human according to claim 2, wherein: the bottom of connecting rod (5) is provided with a plurality of branches (11), and the bottom of a plurality of branches (11) all is provided with ball ring (12), the inside rotation of ball ring (12) is provided with universal ball (13), the bottom of universal ball (13) is provided with bracing piece (14).
5. A test device for detecting gastroesophageal reflux in a human according to claim 1, wherein: the extrusion structure comprises a positioning disc (15), the positioning disc (15) is fixedly arranged at the bottom of the supporting rod (14), an extrusion rod (16) is arranged at the center of the bottom of the positioning disc (15), and an extrusion plug (17) is arranged at the bottom end of the extrusion rod (16).
6. A device for detecting gastroesophageal reflux in a human according to claim 5, wherein: the utility model discloses a reagent bottle, including locating plate (15), extrusion rod (16), supporting plate (19), cap (20), extrusion plug (17), extrusion rod (16) and cap (20), locating plate (15) bottom is located both sides of extrusion rod (16) all is provided with electric putter (18), and the fixed supporting plate (19) that is provided with of output of electric putter (18), the inside of supporting plate (19) is provided with cap (20), cap (20) are installed on the top of reagent tube (4) through the screw thread, extrusion plug (17) are installed in the inside of cap (20), the bottom of extrusion rod (16) extends to the inside of cap (20), and with extrusion plug (17) fixed connection.
7. A test device for detecting gastroesophageal reflux in a human according to claim 1, wherein: the surface of the detection box (1) is movably provided with a box door (21), and the surface of the box door (21) is provided with a glass observation window (22).
8. A test device for detecting gastroesophageal reflux in a human according to claim 1, wherein: the inside of standing groove (2) is provided with shield cover (23), shield cover (23) is at the inside sliding connection of standing groove (2), and the surface of shield cover (23) is provided with draw groove (24).
9. A test device for detecting gastroesophageal reflux in a human according to claim 1, wherein: the bottom of reagent pipe (4) is provided with drip pipe (25), drip pipe (25) and the inside detection card (3) position one-to-one of standing groove (2), and the outside of drip pipe (25) is provided with solenoid valve (26).
10. A method for preparing a quality control product, which is matched with the detection device for detecting gastroesophageal reflux of a human body according to any one of claims 1-9, the method comprising the following steps:
step one, collecting saliva pepsin samples of the oral cavity of a patient, and centrifuging and desizing the saliva pepsin samples to obtain pepsin;
step two, adding protease preservation solution into pepsin to prepare a protease sample;
step three, preparing protease separation liquids with different densities, wherein the density range is 1.083-1.257g/ml, and sequentially adding the protease separation liquids into a centrifuge tube according to the density from large to small to obtain layering liquid;
slowly adding the protease sample into the layering liquid, and centrifuging to obtain layered protease, wherein protease matched with the protease is dispersed in the protease separating liquid with each density;
step five, split charging the layered protease into different test tubes, adding physiological saline into each test tube to obtain protease suspension, adjusting the hemoglobin concentration of the protease suspension to 125g/L, and screening out protease suspension with HbA1c value of 3-5%, thus obtaining quality control product;
step six, adding a buffer solution containing a surfactant into a protease suspension with HbA1c value of 3-5% to obtain a solution with HbA1c value of 3-5% and a protease film dispersed in the solution, removing the protease film through centrifugal treatment, adding at least one of a freeze-drying protective agent or a stabilizer into the solution with HbA1c value of 3-5%, and carrying out freeze-drying or liquid freezing preservation treatment to obtain the pepsin quality control product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311815188.0A CN117844632A (en) | 2023-12-27 | 2023-12-27 | Detection device for detecting gastroesophageal reflux of human body and preparation method of quality control product of detection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311815188.0A CN117844632A (en) | 2023-12-27 | 2023-12-27 | Detection device for detecting gastroesophageal reflux of human body and preparation method of quality control product of detection device |
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| CN117844632A true CN117844632A (en) | 2024-04-09 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202311815188.0A Pending CN117844632A (en) | 2023-12-27 | 2023-12-27 | Detection device for detecting gastroesophageal reflux of human body and preparation method of quality control product of detection device |
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