CN117776391B - Garden sewage treatment method - Google Patents
Garden sewage treatment method Download PDFInfo
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- CN117776391B CN117776391B CN202410199361.7A CN202410199361A CN117776391B CN 117776391 B CN117776391 B CN 117776391B CN 202410199361 A CN202410199361 A CN 202410199361A CN 117776391 B CN117776391 B CN 117776391B
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- 239000010865 sewage Substances 0.000 title claims abstract description 52
- 238000011282 treatment Methods 0.000 title claims abstract description 24
- 238000000034 method Methods 0.000 title claims abstract description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 23
- 244000025254 Cannabis sativa Species 0.000 claims abstract description 20
- 238000006243 chemical reaction Methods 0.000 claims abstract description 6
- 238000004062 sedimentation Methods 0.000 claims abstract description 5
- 238000005273 aeration Methods 0.000 claims abstract description 4
- 229920002752 Konjac Polymers 0.000 claims description 35
- 235000010485 konjac Nutrition 0.000 claims description 35
- 239000011248 coating agent Substances 0.000 claims description 21
- 238000000576 coating method Methods 0.000 claims description 21
- 239000000284 extract Substances 0.000 claims description 20
- 239000007788 liquid Substances 0.000 claims description 17
- 238000010494 dissociation reaction Methods 0.000 claims description 16
- 230000005593 dissociations Effects 0.000 claims description 16
- 108090000790 Enzymes Proteins 0.000 claims description 11
- 102000004190 Enzymes Human genes 0.000 claims description 11
- 101001065065 Aspergillus awamori Feruloyl esterase A Proteins 0.000 claims description 8
- 241000192121 Nitrospira <genus> Species 0.000 claims description 8
- 230000001546 nitrifying effect Effects 0.000 claims description 8
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 7
- 238000000227 grinding Methods 0.000 claims description 7
- 238000012423 maintenance Methods 0.000 claims description 7
- 238000002360 preparation method Methods 0.000 claims description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 6
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 claims description 6
- 238000004880 explosion Methods 0.000 claims description 6
- 238000005507 spraying Methods 0.000 claims description 6
- 241000590020 Achromobacter Species 0.000 claims description 5
- 241000206596 Halomonas Species 0.000 claims description 5
- 210000004209 hair Anatomy 0.000 claims description 5
- 239000000084 colloidal system Substances 0.000 claims description 4
- 238000005520 cutting process Methods 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
- 238000012545 processing Methods 0.000 claims description 3
- 229910000029 sodium carbonate Inorganic materials 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 3
- 241000195493 Cryptophyta Species 0.000 abstract description 12
- 241000251468 Actinopterygii Species 0.000 abstract description 5
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 abstract description 4
- 229910052760 oxygen Inorganic materials 0.000 abstract description 4
- 239000001301 oxygen Substances 0.000 abstract description 4
- 241001148471 unidentified anaerobic bacterium Species 0.000 abstract description 3
- 241000976983 Anoxia Species 0.000 abstract description 2
- 206010002660 Anoxia Diseases 0.000 abstract description 2
- 206010021143 Hypoxia Diseases 0.000 abstract description 2
- 244000207740 Lemna minor Species 0.000 abstract description 2
- 230000007953 anoxia Effects 0.000 abstract description 2
- 239000000725 suspension Substances 0.000 abstract description 2
- 239000003973 paint Substances 0.000 abstract 1
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 18
- 230000000052 comparative effect Effects 0.000 description 14
- 229910052757 nitrogen Inorganic materials 0.000 description 9
- 230000000694 effects Effects 0.000 description 5
- 239000000126 substance Substances 0.000 description 4
- 230000037406 food intake Effects 0.000 description 3
- 238000000746 purification Methods 0.000 description 3
- 241001453369 Achromobacter denitrificans Species 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- 241000252230 Ctenopharyngodon idella Species 0.000 description 2
- MMDJDBSEMBIJBB-UHFFFAOYSA-N [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] Chemical compound [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] MMDJDBSEMBIJBB-UHFFFAOYSA-N 0.000 description 2
- 241001148470 aerobic bacillus Species 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000000969 carrier Substances 0.000 description 2
- 238000006555 catalytic reaction Methods 0.000 description 2
- 238000004821 distillation Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 241000195628 Chlorophyta Species 0.000 description 1
- 241000100611 Halomonas denitrificans Species 0.000 description 1
- 241000605159 Nitrobacter Species 0.000 description 1
- 241000607142 Salmonella Species 0.000 description 1
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 238000010170 biological method Methods 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 238000011221 initial treatment Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000006396 nitration reaction Methods 0.000 description 1
- 238000011056 performance test Methods 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 238000004065 wastewater treatment Methods 0.000 description 1
Landscapes
- Treatment Of Biological Wastes In General (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
The invention relates to the technical field of sewage treatment, and in particular provides a garden sewage treatment method, which comprises the following steps: step one: introducing the garden sewage into a sedimentation tank, adjusting the pH of the garden sewage to 7.5 at 25 ℃, and settling for 6 hours to obtain the sediment-removed garden sewage; step two: introducing the precipitation-removed garden sewage into a biological reaction tank, adding a biological carrier with the mass of 0.4-0.6% of the precipitation-removed garden sewage, and controlling the air-water ratio to be 8 by using an aeration hose: the reaction is completed in 10 hours at the temperature of 1, 25 ℃. The garden sewage treatment method of the invention takes algae removal and edibility into account, provides a natural suspension carrier environment containing oxygen and anoxia through the natural duckweed plants such as the field grass, is matched with the special biological paint of the invention, can be used as a part of ecological landscape while effectively purifying water, can be eaten by fishes after being discharged, and is attached with algae, thereby enlarging the propagation area of anaerobic bacteria under water while removing algae.
Description
Technical Field
The invention relates to the technical field of sewage treatment, in particular to a garden sewage treatment method.
Background
The sewage in gardens mainly comprises domestic sewage and precipitation. The sewage generated in landscape architecture is mainly domestic sewage, so that the sewage contains a large amount of organic matters and the like and has a certain hazard. The basic method for sewage treatment comprises the following steps: physical, biological, chemical, and the like. These wastewater treatment processes often require a combination of applications. The sedimentation treatment is a primary treatment and the biological treatment is a secondary treatment. The core idea is denitrification treatment, sewage denitrification is realized mainly by a biological method, and the core is two biological enzymatic reactions of nitrification and denitrification which are related to each other. In the nitration reaction, the oxygen oxidizes ammonia nitrogen into nitrate nitrogen under the catalysis of biological enzyme secreted by nitrifying bacteria; in the denitrification reaction, the carbon source reduces nitrate nitrogen into nitrogen under the catalysis of biological enzymes secreted by denitrifying bacteria, and the nitrogen escapes from water to realize the aim of sewage denitrification.
The garden sewage treatment has unique requirements: the treatment capacity is small, the treatment is nearby, and the waste is discharged in situ. Algae in a landscape are likely to flood to affect the landscape, and solid matters discharged after sewage treatment are often inedible and are mistakenly eaten by fishes in the landscape to cause loss.
Therefore, a method for treating edible garden sewage which can remove algae is needed.
Disclosure of Invention
In order to solve the problems, the invention aims to provide a garden sewage treatment method.
The invention provides a garden sewage treatment method, which comprises the following steps:
step one: introducing the garden sewage into a sedimentation tank, adjusting the pH of the garden sewage to 7.5 at 25 ℃, and settling for 6 hours to obtain the sediment-removed garden sewage;
Step two: introducing the precipitation-removed garden sewage into a biological reaction tank, adding a biological carrier with the mass of 0.4-0.6% of the precipitation-removed garden sewage, and controlling the air-water ratio to be 8 by using an aeration hose: reacting for 10 hours at the temperature of 1, 25 ℃ to finish treatment;
the preparation method of the biological carrier comprises the following steps:
Taking 5kg of fresh Chinese character 'tian' grass, spraying a coating A with the mass of 4% of the Chinese character 'tian' grass on the leaf surfaces, and spraying a coating B with the mass of 2% of the Chinese character 'tian' grass on the root hairs of the Chinese character 'tian' grass; the coating A comprises the following components in percentage by mass: 3:3:19, nitromonas nitrosamini, nitrospira nitrosamini, nitrifying bacillus vickers and konjak extracts; the coating B comprises the following components in percentage by mass: 1:17, denitrifying achromobacter, denitrifying halomonas and konjak extract;
The preparation method of the konjak extract comprises the following steps:
Cutting fresh konjak tubers into blocks with the size of 1dm 3, and immersing the blocks in dissociation liquid for 6 hours at 48 ℃ to obtain once dissociated konjak blocks; the dissociation liquid comprises 12-16% of feruloyl esterase, 11-14% of C1 enzyme and the balance of pure water; performing steam explosion on the primary dissociated konjak blocks, wherein the steam pressure is 2.2-2.6 MPa, and the maintenance time is 70-100 s, so as to obtain secondary dissociated konjak blocks; adding three times of pure water into the secondary dissociated konjak blocks, adjusting pH to 8.0 with sodium carbonate, grinding for 5min at 90 ℃ with a colloid mill, wherein the processing fineness of the materials is 60um; grinding, adding five times of 95% ethanol, stirring, distilling at 65deg.C under reduced pressure of 600-700 mbar for 170s, and collecting the rest liquid to obtain the rhizoma Amorphophalli extract.
Further, the biological carrier with the mass accounting for 0.5 percent of the precipitation-removed garden sewage is added.
Further, the dissociation liquid comprises 14% of feruloyl esterase by mass.
Further, the dissociation liquid comprises 13% of C1 enzyme by mass fraction.
Further, the steam pressure is 2.4MPa.
Further, the maintenance time is 90s.
Further, the vacuum degree is 650mbar.
The invention has the following beneficial effects:
The invention relates to a garden sewage treatment method, which takes algae removal and edibility into consideration, and is characterized in that natural duckweed plants such as field grass are used for providing a natural suspension carrier environment containing oxygen and anoxia, aerobic bacteria and anaerobic bacteria are respectively attached to leaves and root hairs of the field grass, the field grass can be used as part of ecological landscape while effectively purifying water, the field grass can be eaten by fishes after being discharged, the root hairs of the field grass are easy to attach to algae, the propagation area of underwater anaerobic bacteria can be enlarged while algae removal is carried out, further, oxygen generated by photosynthesis of the field grass is beneficial to growth of the aerobic bacteria, and inorganic nitrogen decomposed by flora and the like can promote growth of the field grass.
The specific biological carrier proportion of the invention can effectively purify water and avoid damaging landscapes due to excessive propagation; the specific strain addition amount and the specific strain addition proportion of the coating A and the specific strain addition proportion of the coating B can not excessively pollute the water body when being matched with nitrogen reduction; the proportion of the dissociation solution is matched with dissociation of loose konjak tissue, and preparation is carried out for subsequent steam explosion and grinding, so that proper residues after distillation are obtained; the pressure and time of the steam explosion of the invention avoid excessively damaging the biological activity of konjak and the viscosity of polysaccharide substances while further dissociating konjak tissues, and provide an adhesion and propagation environment with the field grass for the following mixed strains; the reduced pressure distillation condition of the invention can reduce the antibacterial substances of the konjak extract and avoid the viscosity reduction at the same time.
Detailed Description
In order to more clearly illustrate the overall concept of the present invention, the following describes the overall scheme of the present invention in detail by way of examples; in the following description, numerous specific details are set forth in order to provide a more thorough understanding of the present invention; it will be apparent, however, to one skilled in the art that the invention may be practiced without one or more of these details; in other instances, well-known features have not been described in detail in order to avoid obscuring the invention.
In the invention, a steam explosion machine is purchased from Hebi, a main heavy sentence agricultural machinery manufacturing plant, and the product number is LB-X; the reduced pressure distiller was purchased from Chengshi instruments Inc., zhengzhou, model RE-2000E. The enzyme activities of the C1 enzyme and the feruloyl esterase are about 5 ten thousand U/g; the colloid mill is purchased from the chemical machinery Co., ltd, JM-180; the strains of nitromonas, nitrospira nitrosamini, nitrobacter vickers, achromobacter denitrificans and Salmonella denitrificans are all purchased from biobw platforms, the platform numbers are Bio-67512, bio-73954, bio-67411, bio-78932 and Bio-03835 respectively, the density OD600 of the cultured strains is 30, and the strains are used after eluting the corresponding culture medium.
Unless otherwise specified, the starting components in the examples below are commercially available, and the laboratory apparatus used is a laboratory conventional laboratory apparatus, and the performance test methods are known in the art. The whole operation space environment is 25 ℃, and the air humidity is 30%.
The preferred embodiment is as follows:
Example 1:
the garden sewage treatment method comprises the following steps:
Step one: introducing the garden sewage into a sedimentation tank, adjusting the garden sewage ph to 7.5 at 25 ℃, and settling for 6 hours to obtain the sediment-removed garden sewage;
Step two: introducing the precipitation-removed garden sewage into a biological reaction tank, adding a biological carrier with the mass of 0.5% of the precipitation-removed garden sewage, and controlling the air-water ratio to be 8 by using an aeration hose: reacting for 10 hours at the temperature of 1, 25 ℃ to finish treatment;
the preparation method of the biological carrier comprises the following steps:
Taking 5kg of fresh Chinese character 'tian' grass, spraying a coating A with the mass of 4% on the leaf surfaces of the Chinese character 'tian' grass, and spraying a coating B with the mass of 2% on the root hairs of the Chinese character 'tian' grass; the coating A comprises the following components in percentage by mass: 3:3:19, nitromonas nitrosamini, nitrospira nitrosamini, nitrifying bacillus vickers and konjak extracts; the coating B comprises the following components in percentage by mass: 1:17, denitrifying achromobacter, denitrifying halomonas and konjak extract;
The preparation method of the konjak extract comprises the following steps:
Cutting fresh konjak tubers into blocks with the size of 1dm 3, and immersing the blocks in dissociation liquid for 6 hours at 48 ℃ to obtain once dissociated konjak blocks; the dissociation liquid comprises 14% of feruloyl esterase, 13% of C1 enzyme and the balance of pure water; performing steam explosion on the primary dissociated konjak blocks, wherein the steam pressure is 2.4MPa, and the maintenance time is 90s, so as to obtain secondary dissociated konjak blocks; adding three times of pure water into the secondary dissociated konjak blocks, adjusting pH to 8.0 with sodium carbonate, grinding for 5min at 90 ℃ with a colloid mill, wherein the processing fineness of the materials is 60um; grinding, adding five times of 95% ethanol, stirring, vacuum distilling at 65deg.C under 650mbar for 170s, and collecting the rest liquid to obtain rhizoma Amorphophalli extract.
Examples 2 to 13:
Example 2 differs from example 1 only in that the input mass of the biological carrier is 0.4% of the reprecipitated garden sewage;
Example 3 differs from example 1 only in that the input mass of the biological carrier is 0.6% of the reprecipitated garden sewage;
example 4 differs from example 1 only in that the dissociation liquid comprises 12% by mass of feruloyl esterase;
Example 5 differs from example 1 only in that the dissociation liquid comprises 16% by mass of feruloyl esterase;
example 6 differs from example 1 only in that the dissociation liquid comprises 11% by mass of C1 enzyme;
example 7 differs from example 1 only in that the dissociation liquid comprises 14% by mass of C1 enzyme;
example 8 differs from example 1 only in that the steam pressure is 2.2MPa;
example 9 differs from example 1 only in that the steam pressure is 2.6MPa;
example 10 differs from example 1 only in that the maintenance time is 70s;
Example 11 differs from example 1 only in that the maintenance time is 100s;
example 12 differs from example 1 only in that the vacuum is 600mbar;
example 13 differs from example 1 only in that the vacuum is 700mbar.
Comparative examples 1 to 13:
comparative example 1 differs from example 1 only in that the biological carrier was charged in an amount of 1% by mass of the reprecipitated garden sewage;
Comparative example 2 differs from example 1 only in that the dissociation liquid comprises 20% by mass of feruloyl esterase;
Comparative example 3 differs from example 1 only in that the dissociation liquid comprises 18% by mass of C1 enzyme;
comparative example 4 differs from example 1 only in that the steam pressure was 3.0MPa;
comparative example 5 differs from example 1 only in that the maintenance time is 150s;
comparative example 6 differs from example 1 only in that the vacuum is 800mbar;
Comparative example 7 differs from example 1 only in that coating a comprises a mass ratio of 4:3:3:19, nitromonas nitrosamini, nitrospira nitrosamini, nitrifying bacillus vickers and konjak extracts;
comparative example 8 differs from example 1 only in that coating a comprises a mass ratio of 2:6:3:19, nitromonas nitrosamini, nitrospira nitrosamini, nitrifying bacillus vickers and konjak extracts;
comparative example 9 differs from example 1 only in that coating a comprises a mass ratio of 2:3:6:19, nitromonas nitrosamini, nitrospira nitrosamini, nitrifying bacillus vickers and konjak extracts;
Comparative example 10 differs from example 1 only in that coating a comprises a mass ratio of 2:3:3:40, nitromonas nitrosamini, nitrospira nitrosamini, nitrifying bacillus vickers and konjak extracts;
comparative example 11 differs from example 1 only in that coating B comprises a mass ratio of 6:1:17, denitrifying achromobacter, denitrifying halomonas and konjak extract;
comparative example 12 differs from example 1 only in that coating B comprises a mass ratio of 3:2:17, denitrifying achromobacter, denitrifying halomonas and konjak extract;
comparative example 13 differs from example 1 only in that coating B comprises a mass ratio of 3:1:30 Achromobacter denitrificans, halomonas denitrificans and konjak extract.
Experiments were performed with a throughput of 10m 3: for the blank groups of the treated garden sewage and the untreated garden sewage in each example, measuring the total nitrogen concentration of each treated water, and representing the water purification and denitrification effect by using the total nitrogen concentration of each example, wherein the smaller the total nitrogen concentration is, the better the water purification and denitrification effect is, the result is reserved to the effective number behind the decimal point, the test result is shown in table 1, and the unit is mg/l; the treated water and the biological carrier are discharged into a fishpond of which the depth of 1m is 15m 2 and the length of the grass carp is 12 cm, the total ingestion times of the grass carp to the biological carrier in 6 hours are observed, the edible performance is better as the total ingestion times are more, the test results are shown in table 1, and the unit is times; the treated water and the biological carrier are discharged into a pond with a depth of 1m and a depth of 15m 2 and poured with 1kg of green algae, and the biological carrier is taken after 24 hours to observe the amount of the attached algae, and the description is recorded in table 1.
Table 1: the total nitrogen concentration of the treated garden sewage, the total feeding frequency of fish to the biological carrier and the algae attaching condition of the biological carrier are tested.
As can be seen from the data in table 1, compared with other examples, the method for treating garden sewage according to the embodiment of the invention, in particular, the method for treating garden sewage according to the embodiment 1 of the invention, has the advantages of low total nitrogen concentration of treated water, high total ingestion frequency of fish on biological carriers, and most algae attached to the biological carriers, namely, good water purification and denitrification effects on the basis of good algae removal and edibility effects.
The foregoing is merely exemplary of the present invention and is not intended to limit the present invention; various modifications and variations of the present invention will be apparent to those skilled in the art; any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (1)
1. The garden sewage treatment method is characterized by comprising the following steps of:
Step one: introducing the garden sewage into a sedimentation tank, adjusting the garden sewage ph to 7.5 at 25 ℃, and settling for 6 hours to obtain the sediment-removed garden sewage;
Step two: introducing the precipitation-removed garden sewage into a biological reaction tank, adding a biological carrier with the mass of 0.5% of the precipitation-removed garden sewage, and controlling the air-water ratio to be 8 by using an aeration hose: reacting for 10 hours at the temperature of 1, 25 ℃ to finish treatment;
the preparation method of the biological carrier comprises the following steps:
Taking 5kg of fresh Chinese character 'tian' grass, spraying a coating A with the mass of 4% of the Chinese character 'tian' grass on the leaf surfaces, and spraying a coating B with the mass of 2% of the Chinese character 'tian' grass on the root hairs of the Chinese character 'tian' grass; the coating A comprises the following components in percentage by mass: 3:3:19, nitromonas nitrosamini, nitrospira nitrosamini, nitrifying bacillus vickers and konjak extracts; the coating B comprises the following components in percentage by mass: 1:17, denitrifying achromobacter, denitrifying halomonas and konjak extract;
The preparation method of the konjak extract comprises the following steps:
Cutting fresh konjak tubers into blocks with the size of 1dm 3, and immersing the blocks in dissociation liquid for 6 hours at 48 ℃ to obtain once dissociated konjak blocks; the dissociation liquid comprises 14% of feruloyl esterase, 13% of C1 enzyme and the balance of pure water; performing steam explosion on the primary dissociated konjak blocks, wherein the steam pressure is 2.4MPa, and the maintenance time is 90s, so as to obtain secondary dissociated konjak blocks; adding three times of pure water into the secondary dissociated konjak blocks, adjusting pH to 8.0 with sodium carbonate, grinding for 5min at 90 ℃ with a colloid mill, wherein the processing fineness of the materials is 60um; grinding, adding five times of 95% ethanol, stirring, vacuum distilling at 65deg.C under 650mbar for 170s, and collecting the rest liquid to obtain the rhizoma Amorphophalli extract.
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