CN117706753A - Microscopic device - Google Patents

Abstract

The embodiment of the invention provides a microscopic device, and relates to the technical field of microscopic devices. The microscope device comprises a glass carrier, a first image acquisition assembly, a first lens module, a second image acquisition assembly, a second lens module and an illumination assembly. The glass carrier is used for carrying a detection sample. The first image acquisition assembly is arranged corresponding to the glass carrier. The first lens module is arranged between the glass carrier and the first image acquisition component. The second image acquisition assembly is arranged corresponding to the glass carrier. The second lens module is arranged between the quick-matching book carrying glass frame and the second image acquisition component. An illumination assembly for illuminating the test sample. The method can acquire two groups of images with different magnification factors at the same time, thereby being convenient for use in specific scenes.

Description

Microscopic device

Technical Field

The invention relates to the technical field of microscopes, in particular to a microscope device.

Background

The microscope is an optical instrument composed of one or a plurality of lenses and capable of magnifying microscopic substances such as cells, viruses and the like which cannot be distinguished by naked eyes into a macroscopic object.

However, the existing microscope cannot acquire the fields of view of different magnifications of the two simultaneously, so that the existing microscope is inconvenient to use in some scenes.

Disclosure of Invention

Objects of the present invention include, for example, providing a microscopy apparatus capable of simultaneously acquiring two sets of images at different magnifications, thereby facilitating use in a particular scenario.

Embodiments of the invention may be implemented as follows:

the present invention provides a microscopy apparatus comprising:

the glass carrier is used for carrying the detection sample;

the first image acquisition component is arranged corresponding to the glass carrier;

the first lens module is arranged between the glass carrier and the first image acquisition component;

the second image acquisition component is arranged corresponding to the glass carrier;

the second lens module is arranged between the quick-matching book carrying glass frame and the second image acquisition component and is used for carrying out quick-matching book carrying glass frame.

An illumination assembly for illuminating the test sample.

In an alternative embodiment, the adjustable magnification of the first lens module and the adjustable magnification of the second lens module are different.

In an alternative embodiment, the first image capturing component and the second image capturing component are respectively disposed on two sides of the glass carrier.

In an alternative embodiment, the first image acquisition component and the second image acquisition component are centered or misplaced;

the illumination assembly comprises a first light source and a first light splitting piece;

the beam splitting component is arranged between the first image acquisition component and the first lens module or between the second image acquisition component and the second lens module in an inclined mode;

the first light source corresponds to the light splitting piece, and light emitted by the first light source can irradiate a detection sample borne on the carrier piece through the emission of the first light splitting piece.

In an alternative embodiment, the lighting assembly further comprises a second light source and a second light splitter;

the second light splitting piece is arranged between the first light splitting piece and the first light source, and the second light source is arranged on the other side of the second light splitting piece; wherein one of the first light source and the second light source is for providing bright field illumination and the other is for fluorescent field illumination.

The light emitted by the first light source is refracted by the second light-splitting piece and then irradiates the first light-splitting piece, and then reflected by the first light-splitting piece and then can irradiate a detection sample borne on the glass carrier;

the light emitted by the second light source is reflected by the second light-splitting piece and then irradiates the first light-splitting piece, and then is refracted by the first light-splitting piece and then can irradiate a detection sample borne on the glass carrier.

In an alternative embodiment, the first image capturing component and the second image capturing component are disposed on the same side of the glass carrier and are disposed at intervals in one direction of the glass carrier.

In an alternative embodiment, the lighting assembly includes a third light splitting member, a third light source, a fourth light splitting member, and a fourth light source;

the third light splitting piece is arranged between the first image acquisition assembly and the first lens module, the third light source is arranged corresponding to the third light splitting piece, and light rays emitted by the third light source can be reflected to a detection sample arranged on the glass carrier through the third light splitting piece;

the fourth light splitting piece is arranged between the second image acquisition component and the second lens module, the fourth light source is arranged corresponding to the fourth light splitting piece, and light rays emitted by the fourth light source can be reflected to a detection sample arranged on the glass carrier through the fourth light splitting piece.

In an alternative embodiment, the lighting assembly further comprises a fifth light splitting element, a fifth light source, a sixth light splitting element, and a sixth light source;

the fifth light-splitting component is arranged between the third light-splitting component and the third light source, the fifth light source is arranged corresponding to the fifth light-splitting component, light emitted by the third light source can be refracted to the third light-splitting component through the fifth light-splitting component and then reflected to a detection sample arranged on the glass carrier through the third light-splitting component, and light emitted by the fifth light source can be reflected to the third light-splitting component through the fifth light-splitting component and then reflected to the detection sample arranged on the glass carrier through the third light-splitting component; wherein one of the third light source and the fifth light source is used for providing bright field illumination for the detection sample, and the other is used for providing fluorescent field illumination for the detection sample.

In an alternative embodiment, the lighting assembly is disposed on the other side of the glass carrier.

In an alternative embodiment, the microscopy apparatus further comprises a mirror,

the reflector is arranged on the light emitting side of the first lens assembly, the first image acquisition assembly is arranged on the light emitting side of the reflector, and light rays emitted by the first lens assembly can be reflected to the first image acquisition assembly through the reflector.

The microscopic device provided by the embodiment of the invention has the beneficial effects that:

the first lens module corresponding to the glass carrier and the first image acquisition component corresponding to the first lens module are arranged, and the first image acquisition component can obtain an image with a magnification factor of a detection sample by adjusting the magnification distribution of the first lens module. And through setting up in the second camera lens module that the glass carrier corresponds and the second image acquisition subassembly that corresponds with the second camera lens module, through the magnification of adjustment second camera lens module to the second image acquisition subassembly can acquire the image of another kind of magnification, thereby lets the use more convenient.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are needed in the embodiments will be briefly described below, it being understood that the following drawings only illustrate some embodiments of the present invention and therefore should not be considered as limiting the scope, and other related drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.

FIG. 1 is a schematic diagram of a layout structure of two sides of a microscope device according to an embodiment of the present invention;

FIG. 2 is a schematic diagram of another layout of illumination assemblies under a two-sided layout of a microscope apparatus according to an embodiment of the present invention;

FIG. 3 is a schematic diagram showing a layout of a first layout structure of a microscope device according to an embodiment of the present invention;

FIG. 4 is a schematic diagram of a structure of a second image acquisition assembly disposed adjacent to a side of a microscope device according to an embodiment of the present invention;

FIG. 5 is a schematic illustration showing another arrangement of illumination assemblies for a two-sided arrangement of a microscopy apparatus according to an embodiment of the present invention;

FIG. 6 is a schematic diagram of a layout of a microscope apparatus according to an embodiment of the present invention;

fig. 7 is a schematic diagram of a structure in which an illumination assembly is disposed on the other side of a layout structure on the same side of a microscope device according to an embodiment of the present invention.

Icon: 100-microscopy apparatus; 110-a glass carrier; 131-a first image acquisition component; 133-a first lens module; 135-a second image acquisition component; 137-a second lens module; 150-an illumination assembly; 151-a first light source; 153-a first light splitting member; 155-a second light source; 157-a second light splitting member; 159-a third beam splitter; 161-a third light source; 162-fourth light splitting element; 163-a fourth light source; 164-a fifth light splitting element; 165-a fifth light source; 166-sixth beam splitter; 167-a sixth light source; 171-a mirror; 173-a second mirror.

Detailed Description

For the purpose of making the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is apparent that the described embodiments are some embodiments of the present invention, but not all embodiments of the present invention. The components of the embodiments of the present invention generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations.

Thus, the following detailed description of the embodiments of the invention, as presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.

It should be noted that: like reference numerals and letters denote like items in the following figures, and thus once an item is defined in one figure, no further definition or explanation thereof is necessary in the following figures.

In the description of the present invention, it should be noted that, if the terms "upper", "lower", "inner", "outer", and the like indicate an azimuth or a positional relationship based on the azimuth or the positional relationship shown in the drawings, or the azimuth or the positional relationship in which the inventive product is conventionally put in use, it is merely for convenience of describing the present invention and simplifying the description, and it is not indicated or implied that the apparatus or element referred to must have a specific azimuth, be configured and operated in a specific azimuth, and thus it should not be construed as limiting the present invention.

Furthermore, the terms "first," "second," and the like, if any, are used merely for distinguishing between descriptions and not for indicating or implying a relative importance.

It should be noted that the features of the embodiments of the present invention may be combined with each other without conflict.

Examples

The inventor finds that in cell and bacteria identification, the cell size is larger, the bacteria size is smaller, and the cell and bacteria can be identified and counted in an optimal visual field range, so that the cell and bacteria can be observed under different magnification, however, the conventional microscope only has one lens module, even if a plurality of image acquisition components are provided, the cell and bacteria can only be identified, and the cell and bacteria can not be observed under different magnification, so that the microscope device 100 needs to be debugged repeatedly for observing and identifying the cell and the bacteria, and the convenience of use is affected. For example, in the observation and identification of cellular bacteria in milk, the volume difference is large. In order to shorten the time for identifying and calculating the physical components, it is preferable that the number of cells, bacteria, or the like in the visual field is increased. The smaller the number of pictures is, the better the magnification that needs to be identified and counted. However, the smaller the magnification, the more likely the cell or bacteria will not be identified, so the minimum magnification required to identify somatic cells or milk is optimal for the device identification time. The preferred magnification for observation, identification and counting of somatic cells in milk is 5 times, the observation of bacteria in milk is preferred, and the observation magnification of bacteria in milk is 20 times, the identification is preferred. Meanwhile, as the bacteria and the somatic cells have larger volume difference, the focuses of the bacteria and the somatic cells are not on the same plane, the identification is serial counting, and the total time is the sum of the identification time of a plurality of formed components. It can be seen that when the magnification is large, bacteria and cells can be identified at the same time, but the time spent in identifying cells with large volume is too long, so that the identification and counting time of the whole system is too long.

In order to solve the above-mentioned problems, referring to fig. 1, an embodiment of the present invention provides a microscope apparatus 100, which can better and simultaneously observe and identify multiple types of physical samples with large volume differences in the same detection sample, and can respectively identify and count in parallel, so as to further shorten the time of classification, identification and counting, and make the use more convenient.

Referring to fig. 1 to 7, in the present embodiment, the microscope apparatus 100 includes a glass carrier 110, a first image capturing module 131, a first lens module 133, a second image capturing module 135, a second lens module 137, and an illumination module 150. The glass carrier 110 is used for carrying a test sample, and the test sample is generally prepared into a biochip before testing. The first image acquisition component 131 is disposed corresponding to the glass carrier 110. The first lens module 133 is disposed between the glass carrier and the first image capturing component 131. The second image acquisition assembly 135 is disposed in correspondence to the glass carrier 110. The second lens module 137 is disposed between the fast book carrier 110 and the second image capturing module 135. The illumination assembly 150 is used to illuminate the test sample.

In this embodiment, through the first lens module 133 corresponding to the glass carrier 110 and the first image capturing component 131 corresponding to the first lens module 133, the first image capturing component 131 can obtain an image with a magnification of the detection sample by adjusting the magnification of the first lens module 133. And through setting up in the second lens module 137 that the glass frame 110 corresponds and the second image acquisition subassembly 135 that corresponds with the second lens module, through adjusting the magnification of second lens module 137 to second image acquisition subassembly 135 can acquire the image of another magnification, thereby make the use more convenient.

The biochip is prepared by sequentially solidifying a large number of biological macromolecules such as nucleic acid fragments, multi-skin molecules, even tissue slices, cells and other biological samples on the surface of a support by using methods such as light guide in-situ synthesis or micro sample application to form a dense two-dimensional molecular array, hybridizing the dense two-dimensional molecular array with target molecules in the labeled biological samples to be detected, and rapidly, parallelly and efficiently detecting and analyzing the intensity of hybridization signals by using a specific instrument so as to judge the number of the target molecules in the samples. It has the advantages of high throughput, miniaturization, automation and low cost. And the method can be also used for ultrasonic wave, sulfate, coagulant, electro-deposition method, temperature sedimentation, gravity sedimentation, magnetic particle sedimentation, centrifugal gradient separation sedimentation and the like, so that the sediment of the object to be detected is counted on a specific surface, and layered identification can be adopted without sedimentation.

The first lens assembly and the second lens assembly, which are comprised of lens groups, can magnify the test sample so that the corresponding first image acquisition assembly 131 and second image acquisition assembly 135 obtain a clear and complete image.

It should be noted that the first lens module 133 and the second lens module 137 may be further configured with an auto-focusing module, which can amplify different multiples according to the requirement, so as to quickly obtain an image.

It should be further noted that the first image capturing component 131 and the second image capturing component 135 may be industrial cameras or camera modules. Such as a CCD camera, CMOS camera, 3d imaging module, camera module, etc. Both the CCD camera and the CMOS camera use an image sensor to convert an optical signal into an electrical signal, and then perform digital processing. CCD cameras are divided into an area CCD industrial camera, a linear CCD industrial camera, a three-wire sensor CCD industrial camera, an interweaved transmission CCD industrial camera and a full-width CCD industrial camera. The CMOS camera integrates the photosensor array, the image signal amplifier, the signal reading circuit, the analog-to-digital conversion circuit, the image signal processor and the controller on a single chip.

In the present embodiment, the adjustable magnification of the first lens module 133 and the second lens module 137 is different.

This arrangement can better achieve cost reduction and miniaturization of the microscope device 100.

For example, in the present embodiment, the adjustable magnification of the first lens module 133 may be 0-20 times, and the adjustable magnification of the second lens module 137 may be 20-50 times. The magnification of the first lens module 133 may be 0-50 times, and the magnification of the second lens module 137 may be 50-100 times. Thus, the magnification adjustable intervals of the first lens module 133 and the second lens module 137 are smaller, so that the size of the microscope device 100 can be reduced, the microscope device can be used under multiple scenes, the whole microscope device 100 can achieve larger magnification, for example, under the condition of obtaining a single photo, the adjustable magnification can be 0-50 times, or 0-100 times. And in special use scenes, two images with different magnification can be acquired simultaneously. The prior art cannot realize the different focuses. The technical scheme of the application can also be used for parallelly photographing, identifying and counting cameras with different magnification factors, and the time is greatly shortened compared with the original scheme.

It should be noted that, in other embodiments of the present application, the magnification of the first lens module 133 and the second lens module 137 may be customized according to actual requirements. For example, the first lens module 133 may have a magnification of 0-20 times, and the second lens module 137 may have a magnification of 10-30 times, so that the first lens module 131 and the second lens module 135 may be overlapped in a common magnification range, that is, the first lens module and the second lens module may be used to identify cells at the same time, or may be used in special situations, where one identifies cells and the other identifies a situation with a larger difference in bacteria equal volume. Thus, the microscope device 100 can be more suitable for example, and multi-scene use can be realized.

Second, the first lens module 133 and the second lens module 137 may also use lens modules with the same magnification. For example, the magnification may be adjusted between 0-50 times or 0-100 times. However, this approach leads to an increase in equipment cost and an increase in recognition time. But it also has more advantages, for example, can realize the recognition and the image acquisition of two cell images simultaneously, and two bacterium image detection and recognition, and the recognition and the detection of a cell and a bacterium, can more clearly discern a certain thing to be recognized, also can discern bacterium and cell in parallel to shorten the time, let the application range wider.

Referring to fig. 1 to 5, in one possible layout situation:

the first image acquisition component 131 and the second image acquisition component 135 are respectively arranged at two sides of the glass carrier. That is, with the glass carrier 110 as a plane, one of the first image capturing component 131 and the second image capturing component 135 is disposed on the front surface of the glass carrier, and the other is disposed on the back surface of the glass carrier. The corresponding first lens module 133 and second lens module 137 are also disposed on two sides of the glass carrier 110 correspondingly. For example, the first image capturing component 131 and the first lens module 133 are disposed above the glass carrier 110, and the first lens module 133 corresponds to a position on the glass carrier where the biochip is mounted. The first image acquisition component 131 is disposed above the first lens module 133, and can acquire an image of the biochip through the first lens module 133. The second lens module 137 is disposed below the glass carrier, and corresponds to the biochip mounted on the glass carrier, and the second image acquisition assembly 135 is disposed below the second lens module 137, and can acquire an image of the detection sample on the biochip through the second lens module 137.

Further, the first image capturing component 131 and the second image capturing component 135 are centered or offset.

Referring to fig. 2 and 3, in this embodiment, the first lens module 133 and the second lens module 137 may be disposed on the same vertical line, or may be disposed in a staggered manner, for example, in a width direction, a length direction, a diagonal direction, etc. of the biochip. So long as the first image acquisition component 131 and the second image acquisition component 135 are guaranteed to acquire images of the sample on the biochip.

In this embodiment, the first image capturing component 131 and the first lens module 133 may be in a vertical line, so that the whole microscope device 100 is arranged in the vertical direction. In some embodiments, a reflective element, such as a mirror 171, may be disposed on the light emitting side of the first lens module 133, so that the first image capturing element 131 is disposed on the side of the first lens module 133 to deflect the light, thereby reducing the height of the micro-device 100.

Referring to fig. 4, in this embodiment, the second image capturing element 135 and the second lens module 137 are aligned in a vertical direction, so that the whole microscope device 100 is disposed vertically. In some embodiments, a reflective element, such as a mirror 171, may be disposed on the light emitting side of the second lens module 137, so that the second image capturing element 135 is disposed on the side of the second lens module 137, and the height of the micro-device 100 can be reduced.

In this embodiment, the illumination assembly 150 includes a first light source 151, a first light-splitting member 153, a second light source 155, and a second light-splitting member 157. Wherein one of the first light source 151 and the second light source 155 is used to provide bright field illumination and the other is used for fluorescent field illumination. The lighting assembly 150 may be disposed above, below, and a portion of each above or below the glass-carrying shelf.

For example, when the lighting assembly 150 is disposed above the glass carrier 110:

referring to fig. 1 and 4, the first beam splitter 153 is disposed between the first image capturing module 131 and the first lens module 133. The first light source 151 is disposed at a side of the first light-splitting member 153 corresponding to the first light-splitting member 153. The light emitted by the first light source 151 may be reflected by the first light splitting element 153 to irradiate the detection sample carried on the carrier element. The image light may be amplified by the first lens module 133 and then transmitted by the first light splitting member 153 so as to enter the first image capturing assembly 131, thereby forming an image. The second spectroscopic member 157 is disposed between the first spectroscopic member 153 and the first light source 151, and the second light source 155 is disposed on the other side of the second spectroscopic member 157. The light emitted by the first light source 151 is refracted by the second light-splitting element 157 and then irradiates the first light-splitting element 153, and then reflected by the first light-splitting element 153 and then irradiates the detection sample carried on the glass carrier 110. The light emitted by the second light source 155 is reflected by the second light-splitting element 157 and then irradiates the first light-splitting element 153, and then is refracted by the first light-splitting element 153 and then irradiates the detection sample carried on the glass carrier 110.

For another example, when the lighting assembly 150 is disposed below the glass carrier 110:

the first beam splitter 153 is disposed between the second image capturing module 135 and the second lens module 137. The first light source 151 is disposed at a side of the light splitting member corresponding to the first light splitting member 153. The light emitted by the first light source 151 may be reflected by the first light splitting element 153 to irradiate the detection sample carried on the carrier element. The image light can be amplified by the second lens module 137 and then transmitted by the first light splitting member 153, so that the image light can enter the second image acquisition assembly 135 to form an image. The second spectroscopic member 157 is disposed between the first spectroscopic member 153 and the first light source 151, and the second light source 155 is disposed on the other side of the second spectroscopic member 157. The light emitted by the first light source 151 is refracted by the second light-splitting element 157 and then irradiates the first light-splitting element 153, and then reflected by the first light-splitting element 153 and then irradiates the detection sample carried on the glass carrier 110. The light emitted by the second light source 155 is reflected by the second light-splitting element 157 and then irradiates the first light-splitting element 153, and then is refracted by the first light-splitting element 153 and then irradiates the detection sample carried on the glass carrier 110.

Also, for example, the first spectroscopic member 153 and the first light source 151 are disposed above, and the second spectroscopic member 157 and the second light source 155 are disposed below:

the first beam splitter 153 is disposed between the first image capturing component 131 and the first lens module 133. The first light source 151 is disposed at a side of the first light-splitting member 153 corresponding to the first light-splitting member 153. The second beam splitter 157 is disposed between the second image capturing module 135 and the second lens module 137. The second light source 155 is disposed at a side of the second spectroscopic member 157 corresponding to the first spectroscopic member 153. The light emitted from the first light source 151 may be reflected to the detection sample through the first light splitting member 153, the light emitted from the second light source 155 may be reflected to the detection sample through the second light splitting member 157, and the image light may be transmitted through the first light splitting member 153 and the second light splitting member 157, thereby causing the first image capturing assembly 131 and the second image capturing assembly 135 to obtain images.

Referring to fig. 2, 3 and 4, for example, the illumination assemblies 150 are divided into two groups, each group is provided with a first light source 151 and a second light source 155, and are respectively disposed on two sides.

Image acquisition in both bright field and fluorescent field scenarios can be achieved by the arrangement of illumination assembly 150 described above. The fluorescent field is provided for the purpose of generally staining a test sample with a specific stain when preparing a biochip, and exciting the stain when fluorescent light irradiates the sample to excite the fluorescent light, so that different kinds of cells in the sample can be distinguished.

Referring to fig. 6 and 7, in another possible layout state:

the first image capturing assembly 131 and the second image capturing assembly 135 are disposed on the same side of the glass carrier 110 and are disposed at intervals in one direction of the glass carrier 110.

In this embodiment, for example, the first image capturing component 131 and the second image capturing component 135 are both disposed above or below the glass carrier. The first lens module 133 is disposed between the first image capturing assembly 131 and the glass carrier 110, and the second lens module 137 is disposed between the second image capturing assembly 135 and the glass carrier 110. The glass holders 110 may be disposed at intervals in one direction of the glass holders 110, or may be disposed at intervals in the length direction of the glass holders 110, or may be disposed at intervals in the width direction, or may be disposed at intervals in the diagonal direction. Typically spaced diagonally.

In the same-side layout state, the lighting assembly is arranged in the following ways:

referring to fig. 6, in one embodiment, the illumination assembly includes a third light splitter 159, a third light source 161, a fourth light splitter 162, and a fourth light source 163. The third light splitting element 159 is disposed between the first image capturing element 131 and the first lens module 133, and the third light source 161 is disposed on a side of the third light splitting element 159, so that light emitted by the third light source 161 can be reflected by the third light splitting element 159 to a detection sample mounted on the glass carrier 110. The fourth light-splitting component 162 is disposed between the second image capturing component 135 and the second lens module 137, and the fourth light source 163 is disposed corresponding to the fourth light-splitting component 162, and the light emitted by the fourth light source 163 can be reflected to the detection sample mounted on the glass carrier 110 by the fourth light-splitting component 162.

In the case of the above-described arrangement of the illumination assembly 150, the third light source 161 and the fourth light source 163 may both be LED light sources providing a bright field, or may both be fluorescent light sources providing a fluorescent field, or may be one LED light source providing a bright field, and the other fluorescent light source (excitation light source) providing fluorescent field illumination. For convenience and ease of use, and correspondence, in the case of the above-described light assembly arrangement, the lighting assembly further includes a fifth light splitter 164, a fifth light source 165, a sixth light splitter 166, and a sixth light source 167. The fifth light-splitting element 164 is disposed between the third light-splitting element 159 and the third light source 161, the fifth light source 165 is disposed at one side of the fifth light-splitting element, the light emitted by the third light source 161 can be refracted (transmitted) to the third light-splitting element 159 by the fifth light-splitting element 164, then reflected by the third light-splitting element 159 to the detection sample mounted on the glass carrier 110, and the light emitted by the fifth light source 165 can be reflected by the fifth light-splitting element 164 to the third light-splitting element 159 and then reflected by the third light-splitting element 159 to the detection sample mounted on the glass carrier 110. One of the third light source 161 and the fifth light source 165 is used for providing bright field illumination for the detection sample, and the other is used for providing fluorescent field illumination for the detection sample. One for each such group.

Of course, in other embodiments of the present application, one of the third light source 161, the fourth light source 163, the fifth light source 165 and the sixth light source 167 may be an LED light source providing a bright field, and the other three may be excitation light sources providing fluorescent fields of different colors. Thus saving more cost and improving the convenience of use.

The above-described arrangement of the illumination assembly 150 allows the overall height of the microscope device 100 to be reduced and the integration to be increased.

Referring to fig. 7, in some arrangements of the lighting assemblies 150 of this embodiment, the lighting assemblies 150 are disposed on the other side of the glass carrier 110. I.e., below the glass carrier 110. This increases the overall height of the microscopy apparatus 100, but saves on the number of optical components, e.g., reduces the use of beam splitters.

In an embodiment, to reduce the overall height of the microscopy apparatus 100, this may be achieved by adding a mirror 171. The microscope device 100 further includes a mirror 171 and a second mirror 173, the mirror 171 is disposed on the light emitting side of the first lens assembly, the first image capturing assembly 131 is disposed on the light emitting side of the mirror 171, and the light emitted from the first lens module 133 can be reflected to the first image capturing assembly 131 by the mirror 171. This may allow the first image capturing assembly 131 to be formed on the side. The second mirror 173 is disposed on the light emitting side of the second lens assembly, the second image capturing assembly 135 is disposed on the light emitting side of the second mirror 173, and the light emitted from the second lens module 137 can be reflected to the second image capturing assembly 135 by the second mirror 173. This may allow the second image acquisition assembly 135 to be formed laterally.

It should be further noted that the present embodiment further includes some filtering elements, correcting elements, etc., which may be disposed on the light emitting side of the light source and the light entering side of the image capturing assembly as required, so as to implement the filtering function. Next, the first, second, third, and the like optical splitters 153, 157, 159 may be beam splitters or dichroic mirrors so that light propagates according to a predetermined optical path.

Of course, in some embodiments of the present application, the illumination assembly 150 may also implement illumination of bright scenes and fluorescent scenes by using multiple-wavelength and multiple-in-one beads in combination with multiple-wavelength bandpass filters.

It should be noted that, the multi-wavelength all-in-one lamp bead is to combine the LED lamp sources with different wavelengths into a light source system through the optical element, and after the light source with a plurality of narrow-band filters intersects with the target sample, different fluorescent dyes and protein markers can be selectively started and excited. The fluorescent microscope has higher efficient fluorescent imaging quality, and the traditional fluorescent microscope mainly uses a mercury gas lamp or a laser as a light source, so that the irradiation time is longer, the sample light is easily damaged, and the imaging quality is influenced. The multiband LED fluorescent microscope adopts a plurality of LED lamp sources with short wavelengths to form a light source system, so that the power is smaller, the wavelength selection is more accurate, and the imaging process is more efficient and accurate. The multi-band LED fluorescent microscope has more various functions, and can select different excitation wavelengths, so that a plurality of marker molecules can be detected simultaneously, and multiple dyeing imaging is realized. Meanwhile, the operation is simple, compared with the operation of the traditional fluorescent microscope such as a mercury lamp, a laser and the like, the multi-band LED fluorescent microscope is more convenient to use and maintain, and special optical adjustment and alignment operation are not needed.

In summary, the working principles and advantages of the microscope device 100 provided in the embodiments of the present invention include, for example:

the application discloses a first lens module 133 corresponding to a glass carrier 110 and a first image acquisition component 131 corresponding to the first lens module 133, wherein the first image acquisition component 131 can obtain an image with a magnification of a detection sample by adjusting the magnification distribution of the first lens module 133. And through setting up in the second lens module 137 that the glass frame 110 corresponds and the second image acquisition subassembly 135 that corresponds with the second lens module, through adjusting the magnification of second lens module 137 to second image acquisition subassembly 135 can acquire the image of another magnification, thereby make the use more convenient.

The present invention is not limited to the above embodiments, and any changes or substitutions that can be easily understood by those skilled in the art within the technical scope of the present invention are intended to be included in the scope of the present invention. Therefore, the protection scope of the invention is subject to the protection scope of the claims.

Claims (10)

1. A microscopy apparatus, comprising:

a glass carrier (110) for carrying a test sample;

a first image acquisition assembly (131) arranged in correspondence to the glass carrier (110);

a first lens module (133) disposed between the glass carrier (110) and the first image acquisition assembly (131);

a second image acquisition assembly (135) arranged in correspondence to the glass carrier (110);

a second lens module (137) disposed between the glass carrier (110) and the second image acquisition assembly (135), and;

an illumination assembly (150) for illuminating the test sample.

2. The microscopy apparatus according to claim 1, wherein the adjustable magnification of the first lens module (133) and the second lens module (137) is different.

3. The microscopy apparatus according to claim 1, wherein the first image acquisition component (131) and the second image acquisition component (135) are arranged on both sides of the glass carrier (110), respectively.

4. A microscopy apparatus according to claim 3, wherein the first image acquisition assembly (131) and the second image acquisition assembly (135) are arranged in a centered or offset manner;

the lighting assembly (150) comprises a first light source (151) and a first light splitting member (153);

the beam splitting component is arranged between the first image acquisition component (131) and the first lens module (133) or obliquely arranged between the second image acquisition component (135) and the second lens module (137);

the first light source (151) corresponds to the light splitting component, and light emitted by the first light source (151) can irradiate a detection sample carried on the glass carrier (110) through reflection of the first light splitting component (153).

5. The microscopy apparatus of claim 4, wherein the illumination assembly further comprises a second light source (155) and a second light splitting member (157);

the second light-splitting member (157) is disposed between the first light-splitting member (153) and the first light source (151), and the second light source (155) is disposed on the other side of the second light-splitting member (157); wherein one of the first light source (151) and the second light source (155) is for providing bright field illumination and the other is for fluorescent field illumination;

the light emitted by the first light source (151) is refracted by the second light-splitting component (157) and then irradiates the first light-splitting component (153), and then is reflected by the first light-splitting component (153) and then can irradiate a detection sample carried on the glass carrier (110);

the light emitted by the second light source (155) is reflected by the second light splitting component (157) and then irradiates the first light splitting component (153), and then is refracted by the first light splitting component (153) and then can irradiate a detection sample carried on the glass carrier (110).

6. The microscopy apparatus of claim 1, wherein the first image acquisition assembly (131) and the second image acquisition assembly (135) are disposed on the same side of the glass carrier (110) and are spaced apart in one direction of the glass carrier (110).

7. The microscopy apparatus of claim 6, wherein the illumination assembly comprises a third light splitting member (159), a third light source (161), a fourth light splitting member (162), and a fourth light source (163);

the third light splitting component (159) is arranged between the first image acquisition component (131) and the first lens module (133), the third light source (161) is arranged corresponding to the third light splitting component (159), and light emitted by the third light source (161) can be reflected to a detection sample arranged on the glass carrier (110) through the third light splitting component (159);

the fourth light splitting component (162) is disposed between the second image acquisition component (135) and the second lens module (137), the fourth light source (163) is disposed corresponding to the fourth light splitting component (162), and light emitted by the fourth light source (163) can be reflected to a detection sample mounted on the glass carrier (110) through the fourth light splitting component (162).

8. The microscopy apparatus of claim 7, wherein the illumination assembly further comprises a fifth light splitting member (164), a fifth light source (165), a sixth light splitting member (166), and a sixth light source (167);

the fifth light-splitting component (164) is disposed between the third light-splitting component (159) and the third light source (161), the fifth light source (165) is disposed corresponding to the fifth light-splitting component, light emitted by the third light source (161) can be refracted to the third light-splitting component (159) through the fifth light-splitting component (164), and then reflected to a detection sample mounted on the glass carrier (110) through the third light-splitting component (159), and light emitted by the fifth light source (165) can be reflected to the third light-splitting component (159) through the fifth light-splitting component (164), and then reflected to a detection sample mounted on the glass carrier (110) through the third light-splitting component (159); wherein one of the third light source (161) and the fifth light source (165) is for providing bright field illumination for the test sample and the other is for providing fluorescent field illumination for the test sample.

9. The microscopy apparatus of claim 6, wherein the illumination assembly (150) is disposed on the other side of the glass carrier (110).

10. The microscopy device according to any of claims 1-9 further comprising a mirror (171),

the reflecting mirror (171) is arranged on the light emitting side of the first lens module (133), the first image acquisition component (131) is arranged on the light emitting side of the reflecting mirror (171), and the light emitted by the first lens module (133) can be reflected to the first image acquisition component (131) through the reflecting mirror (171).