CN117572625B - Multipath microscopic imaging device and method - Google Patents
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- 239000000725 suspension Substances 0.000 claims description 8
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- 230000000968 intestinal effect Effects 0.000 claims description 6
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
- G02B21/361—Optical details, e.g. image relay to the camera or image sensor
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
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- G02B21/241—Devices for focusing
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Abstract
The multi-path microscopic imaging device and the multi-path microscopic imaging method comprise an image sensor A, an image sensor B, a lens assembly, an imaging light source and a spectroscope A; the imaging light source is used for irradiating the target object; the object-to-lens assembly is an imaging light path; the imaging light path is split by the spectroscope A and is divided into two light paths, and the image sensor A images on one light path; the image sensor B images on the other light path. In the application, the inventor proposes that the time for taking pictures can be reduced by taking images of different positions of the same focal plane or taking images of different focal planes by using a plurality of image sensors.
Description
Technical Field
The application belongs to the technical field of microscopic imaging images, and particularly relates to a multipath microscopic imaging device and method.
Background
The applicant has proposed a series of Chinese patents, such as
1. CN2020112669290, "cell analysis methods and systems and quantification methods and systems";
2. CN2020112669182, "cell suspension sample imaging methods and systems and kits";
3. CN2022104799126, "microscopic image acquisition device quick focusing method and microscopic image acquisition method";
4. CN2023100423151, "blood imaging analysis system and method";
5. CN2023118576607, "marked light assisted microimaging method and apparatus";
the novel technical scheme is used for measuring the content of the target substances in blood, urine and feces, and a novel technical route is developed for detecting the tangible components in the suspension.
The pixel point of the image sensor in the digital microscope is fixed, in the microscopic imaging process, the larger the magnification factor is, the smaller the field of view of the digital camera is, the 40-time magnifying glass is used, the area shot by the digital camera is approximately in a section with the width of 0.2 millimeter and the length of 0.3 millimeter, if the height of the accommodating cavity of the detection chip is 0.2 millimeter, the shooting volume of one picture is 0.012 cubic millimeter, and most detection scenes have requirements on the sample volume of a detection object, so that a plurality of images at different positions or on different focal planes are required to be shot, and in many times, the number of images required to be shot exceeds 1000, and long time is required to be spent, so that the timeliness of detection is seriously affected. Many times, detecting objects in a sample, such as cells, bacteria, etc., requires a detection period of time exceeding a set period of time, and the cell membrane or rupture, resulting in an incorrect measurement of the sample.
Disclosure of Invention
In the application, the inventor proposes that the time for taking pictures can be reduced by taking images of different positions of the same focal plane or taking images of different focal planes by using a plurality of image sensors.
The multi-path microscopic imaging device comprises an image sensor A, an image sensor B, a lens assembly, an imaging light source and a spectroscope A; the imaging light source is used for irradiating the target object; the object-to-lens assembly is an imaging light path; the imaging light path is split by the spectroscope A and is divided into two light paths, and the image sensor A images on one light path; the image sensor B images on the other light path; the focal planes corresponding to the imaging of the image sensor A and the imaging of the image sensor B are different, and the different focal planes are imaged respectively; the target object is a detection chip, and a sample accommodating cavity is formed in the detection chip; the image sensors respectively image different detection targets in the sample accommodating cavity.
The multipath microscopic imaging device also comprises a spectroscope B and an image sensor C; and the spectroscope B divides one light path into a third light path, and the image sensor C images on the third light path.
The multipath microscopic imaging device further comprises a spectroscope C and a marking light component; the spectroscope C divides one light path into a marking light path, the marking light component emits marking light, a marking pattern is formed on a detection target through the marking light path, and the marking pattern is imaged on the image sensor A or the image sensor B through the imaging light path to form a marking image.
A multipath microscopic imaging device can be an image sensor filter plate, and imaging corresponds to different wavelengths.
According to the multipath microscopic imaging device, the detection chip accommodates the blood diluent sample, and the image sensor respectively performs focusing imaging on focal planes of white blood cells, red blood cells or platelets at the same time or at different times.
According to the multipath microscopic imaging device, the detection chip contains the urine sample or the urine concentrated sample or the urine diluted sample, and the image sensor respectively performs focusing imaging on focal planes of the tubular type, the crystal, the cell and/or the pathogenic microorganism at the same time or at different times.
According to the multipath microscopic imaging device, the detection chip contains the fecal suspension, and the image sensor respectively performs focusing imaging on focal planes of parasitic ova, intestinal protozoa, starch particles, lipid droplets, plant fibers, myofibers, cells and/or pathogenic microorganisms at the same time or at different times.
The multipath microscopic imaging device further comprises a Z-axis adjusting assembly, wherein the Z-axis adjusting assembly is used for bearing an image sensor, a spectroscope and a lens assembly, and the Z-axis adjusting assembly is used for adjusting the distance between a target object and the lens assembly.
The multipath microscopic imaging device further comprises a distance adjusting component, wherein the distance adjusting component is used for bearing the image sensor A or the image sensor B, and the distance between the image sensor and the lens component is adjusted by the distance adjusting component.
In the multi-path microscopic imaging device, the distance adjusting component is borne on the Z-axis adjusting component or the distance adjusting component is not borne on the Z-axis adjusting component.
The multi-path microscopic imaging device comprises a marking light component and a light source, wherein the marking light component comprises a laser emitting device; or the marking light component comprises an LED light source which emits red light, green light, blue light or yellow light; or the marker light assembly includes a bulb.
The marking light assembly comprises a marking light source and a perforated light shielding plate, wherein the perforated light shielding plate comprises more than 3 holes; the light emitted by the marking light source passes through the shading plate with holes to form more than 3 light spots on the surface of the target object; imaging the light spot on an image sensor; and judging the perpendicularity of the surface of the target object and the light path by judging whether the mark image is in the light spot or not or the definition.
A multipath microscopic imaging method divides a microscopic imaging light path into two paths through a spectroscope A, and the two paths of microscopic imaging light rays are imaged on an image sensor A or an image sensor B respectively; the microscopic imaging target is a detection chip, and the detection chip comprises a sample accommodating cavity; the image sensors respectively image different detection targets in the sample accommodating cavity, which can be different in distance between the image sensors, and respectively image different focal planes; different lens groups are arranged on a microscopic imaging optical path, and different image sensors respectively image different focal planes; it may be that the image sensor is provided with an adjustable distance means, which image sensor may image different focal planes.
The multi-path microscopic imaging method; and (3) dividing one microscopic imaging light path into a third light path through the spectroscope B, and imaging the image sensor C on the third light path.
The multi-path microscopic imaging method; and (3) dividing one microscopic imaging light path into a third light path through a spectroscope C, and arranging a marking light assembly on the third light path.
A multipath microscopic imaging method; the imaging device can be an image sensor, and imaging corresponds to different wavelengths.
The multi-path microscopic imaging method; the microscopic imaging target is a detection chip, and the detection chip comprises a sample accommodating cavity; the image sensor images different detection targets in the sample accommodating cavity.
According to the multipath microscopic imaging method, the detection chip accommodates a blood diluent sample, and the image sensor respectively performs focusing imaging on focal planes of white blood cells, red blood cells or platelets at the same time or at different times.
According to the multipath microscopic imaging method, the detection chip accommodates a urine sample or a urine concentrated sample or a urine diluted sample, and the image sensor respectively performs focusing imaging on focal planes of the tubular, crystalline, cellular and/or pathogenic microorganisms at the same time or at different times.
According to the multipath microscopic imaging method, the detection chip contains fecal suspension, and the image sensor respectively performs focusing imaging on focal planes of parasitic ova, intestinal protozoa, starch particles, lipid droplets, plant fibers, myofibers, cells and/or pathogenic microorganisms at the same time or at different times.
In the multipath microscopic imaging method, the marking light component comprises a laser emitting device, and the marking light is laser; or the marking light component comprises an LED light source which emits red light, green light, blue light or yellow light; or the marker light assembly includes a bulb.
In the multipath microscopic imaging method, the marking light assembly comprises a marking light source and a perforated light shielding plate, wherein the perforated light shielding plate comprises more than 3 holes; the light emitted by the marking light source passes through the shading plate with holes to form more than 3 light spots on the surface of the target object; imaging the light spot on an image sensor; and judging the perpendicularity of the surface of the target object and the light path by judging whether the mark image is in the light spot or not or the definition.
The technical effects of the technical scheme include: the plurality of image sensors are used for shooting images of different positions of the same focal plane, so that images with larger areas can be shot at one time.
The technical effects of the technical scheme include: the images of different focal planes are shot by a plurality of image sensors, so that targets with different diameters can be shot at the same time, and the time for shooting pictures can be reduced by times.
The technical effects of the technical scheme include: the adoption of the marking light assembly can quickly carry out focusing adjustment.
The technical effects of the technical scheme include: whether the marking light is on a focal plane which is wanted to be focused or not is analyzed, so that the focusing can be accurately performed, the complexity of focusing software is reduced, and a complex focusing algorithm is basically not needed.
The technical effects of the technical scheme include: the object distance is adjusted, the focusing marking light is adjusted, and the height of the cavity of the test chip is accurately measured through the change of the light focusing position of the marking light, so that the height of the cavity can be tested on line.
The technical effects of the technical scheme include: according to the light intensity of the mark patterns obtained by focusing different surfaces of the chip, the position of the current surface can be judged, and the initial position can be quickly judged.
The technical effects of the technical scheme include: the large images are spliced directly through the images of different light paths, the traditional camera is directly adopted, and a driving device of the image sensor is not required to be newly designed.
The technical effects of the technical scheme include: by presetting different focal planes, the focal planes of white blood cells, red blood cells and platelets can be rapidly positioned.
The technical effects of the technical scheme include: the distance between the image sensors can be adjusted, the distance between the image sensors and the object distance can be fixed, flexibility is maintained, and the area of a shot image can be calculated by using a fixed magnification.
The technical effects of the technical scheme include: through laser, can form stable mark pattern, through light intensity, facula size, have the facula, can accurate judgement focusing effect, simple high-efficient.
The technical effects of the technical scheme include: the laser measuring chip cavity height can improve the measuring precision to more than 0.1 micrometer, and the measuring precision can be greatly improved.
The technical effects of the technical scheme include: the marking patterns can be formed on the transparent upper and lower surfaces, so that focusing can be performed on the transparent surfaces, and the problem that a conventional microscope cannot focus on a transparent object is solved.
The technical effects of the technical scheme include: the marking pattern can be formed on transparent upper and lower surfaces so that focusing can be performed on the transparent surfaces to find a measurement standard for measuring cells or other objects.
The technical effects of the technical scheme include: different filters are arranged in front of different image sensors, so that the test of optical absorption intensity can be performed.
Drawings
FIG. 1 is a schematic block diagram of a multiple microscopy imaging means;
FIG. 2 is a schematic diagram of the internal modules of an imaging device with two beamsplitters;
FIG. 3 is a schematic view of the optical path of a tagged optical component;
FIG. 4 is a schematic diagram of two image sensor imaging stitching;
FIG. 5 is a schematic diagram of an optical path for imaging a detection chip;
FIG. 6 is a schematic diagram of another path of light for imaging a detection chip;
FIG. 7 is a schematic diagram of an image sensor strip spacing adjustment function;
FIG. 8 is a schematic diagram of an image sensor strip spacing adjustment function;
FIG. 9 is a schematic diagram of a marking light unit implementation;
FIG. 10 is a schematic diagram of an implementation of a marking pattern for a marking light;
FIG. 11 is a schematic representation of a erythrocyte microscopic imaging of a marked spot.
Detailed Description
The present application is described in further detail below with reference to the accompanying drawings. The following description of the preferred embodiments of the present application is not intended to limit the present application. The description of the preferred embodiments of the present application is merely illustrative of the general principles of the application. The numbers "first", "second" and "a" and "B" in the present application are for convenience of description only, and do not represent a time or space sequence relationship, and the combination of letters and numbers "TA", "TB" and "H" in the present application are for convenience of description only, and the specific meaning is determined by the specific vocabulary referred to.
Referring to FIG. 1, a multi-path microscopic imaging device is shown with an internal functional schematic diagram, an image sensor A121, an image sensor B122, a lens assembly 160, an imaging light source 180, and a beam splitter A130; the imaging light source is used for irradiating the target object; the object-to-lens assembly is an imaging optical path 161; the imaging light path is split by the spectroscope A and is divided into two light paths, and the image sensor A images on one light path 162; the image sensor B images on the other light path 163.
Image sensors mainly include two major types, namely, a Charge-Coupled Device (CCD) and a Complementary Metal Oxide Semiconductor (CMOS) Device (Complementary Metal-Oxide Semiconductor).
The pixels of the image sensor are fixed, and during microscopic imaging, the image sensor can image objects near the focal plane 170 due to the depth of field, and can image on different focal planes by adjusting the object distance 190 through the Z-axis adjusting component. The image analysis component 111 is for analyzing images obtained by the image sensor.
The image sensor has fixed resolution, effective pixel points are fixed, in the test process, a plurality of test items are tested, the number of samples to be shot is large, if 1000 microscopic pictures with the same or different focal planes are required to be shot, more time is required, the position is frequently moved or the shooting focal plane is adjusted in the shooting process, and the state of the samples in a suspended state is also influenced.
If one image sensor is added to shoot images of different positions of the same focal plane or shoot images of different focal planes, 1000 shooting actions can be reduced to 500; if there are 4 image sensors, the photographing action is reduced to 250, and the photographing time period is greatly reduced.
Referring to fig. 2, a multi-path microscopic imaging device further includes a beam splitter B230 and an image sensor C223; and the spectroscope B divides one light path into a third light path, and the image sensor C images on the third light path.
The more optical paths are split, the more the number of spectroscopes is needed, the greater the attenuation of light passing through the spectroscopes, and the different numbers of spectroscopes and image sensors are required to be arranged according to the characteristics of the detected target object.
Referring to fig. 3, a multi-path microscopic imaging device further includes a beam splitter C330 and a marking optical component 331; the spectroscope C divides one light path into a marking light path, the marking light component emits marking light, a marking pattern is formed on a detection target through the marking light path, and the marking pattern is imaged on the image sensor A321 or the image sensor B322 through an imaging light path to form a marking image.
By adopting the marking light component, focusing adjustment can be rapidly carried out, one of the image sensor A or the image sensor B can be provided with a fixed distance, and the lower surface of the cavity of the detection chip can be rapidly found out through the marking light to serve as a reference surface for measurement.
Referring to fig. 9, in an embodiment of a multi-channel microimaging apparatus, a marking light assembly includes a marking light source, a perforated mask, and the perforated mask includes more than 3 holes; the light emitted by the marking light source passes through the shading plate with holes to form more than 3 light spots on the surface of the target object; the light spot is imaged on an image sensor; and judging the perpendicularity of the surface of the target object and the light path by judging whether the mark image is in the light spot or not or the definition.
Microscopic imaging, the perpendicularity of the detection chip and the microscopic imaging unit has close relation to target searching and measurement, and if the detection chip is not perpendicular, due to the depth of field, targets in a plurality of positions in one image can not be imaged and observed, or the cells of part of the imaging image are large, and the cells of part of the imaging image are small.
As shown in fig. 10, the aperture mask has 5 apertures, and if the surface of the object is not perpendicular to the optical path, the imaging states of the 4 apertures on the periphery are not consistent, and the imaging states of the 4 apertures can be adjusted by adjusting the device for perpendicularity.
As shown in fig. 11, if the marker light set is turned on during microscopic imaging, a blood cell imaging image with a light spot can be obtained, and whether the image is qualified or not is judged by judging the form of the light spot.
In the above-mentioned auxiliary microscopic imaging device with marking light, the imaging light source is turned on or off during the period when the marking light component emits marking light.
It may be that the microscopic imaging unit irradiates the object through the imaging light source, and the marking light assembly turns on or off the marking light during microscopic imaging of the object obtained by the image sensor.
It may be that the imaging light source emits light at a wavelength different from or partially the same as the wavelength of the marking light emitted by the marking light assembly.
The imaging light source and the marking light component have different wavelengths, so that marking light can be distinguished in an image, and the light with different wavelengths also has detection functions, such as purple light and infrared light, when a target is detected, namely, the marking light can be used as measuring light in some measuring scenes.
In other embodiments, the marking light assembly includes a laser light emitting device, the marking light being a laser light; or the marking light component comprises an LED light source which emits red light, green light, blue light or yellow light; or the marker light assembly includes a bulb.
A multipath microscopic imaging device can be characterized in that an image sensor images different positions on the same focal plane respectively.
The image sensor can respectively image the same focal plane, and the image sensor is connected with the filter plate, and the imaging corresponds to different wavelengths.
The imaging device can be a multipath microscopic imaging device, the focal planes corresponding to imaging of the image sensor A and the image sensor B are different, and different focal planes are imaged respectively.
As shown in fig. 4, after passing through the spectroscope, the two image sensors respectively correspond to two parts under the microscope field of view for imaging, as shown in the figure, the area 411 and the area 412 respectively correspond to two areas with the length of 0.3 millimeter and the width of 0.2 millimeter, the focal plane shot by one image sensor is 0.3 millimeter long and the width of 0.2 millimeter, the corresponding area of the two image sensors is 0.3 millimeter long and the width of 0.4 millimeter, the two image sensors can obtain the area twice as large as one shot, the two image sensors are respectively positioned in the two cameras, each camera is provided with an independent driving device, and the two parts of one microscope field of view can be accurately aligned through the allocation of a mechanical device. It is of course also possible to splice a plurality of image sensors at one location, which requires the drive means of the image sensors to be re-developed.
As shown in fig. 5 and 6, in a multi-path microscopic imaging device, a target is a detection chip, and a sample accommodating cavity is formed in the detection chip; the image sensor images different detection targets in the sample accommodating cavity.
As shown in the figure, the detection chip includes white blood cells and red blood cells, the diameter of the white blood cells is larger than that of the red blood cells, and as shown in fig. 5, the optical path of the image sensor 521 corresponds to the focal plane 270. As shown in fig. 7, the optical paths of the image sensor 522 correspond to the focal plane 271, and in one imaging process, two images focused on red blood cells and focused on white blood cells can be obtained, and two imaging optical paths with different focal planes are formed through the same lens group 560, so that images of two layers can be captured at a time.
A multipath microscopic imaging device comprises a detection chip for accommodating a blood diluent sample, and an image sensor for respectively carrying out focusing imaging on focal planes of white blood cells, red blood cells or platelets at the same time or at different times.
Blood cells are mainly classified into white blood cells, red blood cells or platelets, the 3 cells have definite radius distinction, and the detection can be quickened by arranging different image sensors to photograph at different cell radius positions respectively.
A multipath microscopic imaging device comprises a detection chip for accommodating a urine sample or a urine concentrated sample or a urine diluted sample, and an image sensor for respectively carrying out focusing imaging on focal planes of a tubular type, a crystal, a cell and/or a pathogenic microorganism at the same time or at different times.
The urine contains a plurality of detection components, and the detection can be quickened by arranging different image sensors to photograph at the radius positions of the detection components respectively.
A multipath microscopic imaging device comprises a detection chip for accommodating fecal suspension, and an image sensor for simultaneously or non-simultaneously focusing and imaging focal planes of parasitic ova, intestinal protozoa, starch particles, lipid droplets, plant fibers, myofibers, cells and/or pathogenic microorganisms.
The fecal suspension contains various detection components, and the detection can be quickened by arranging different image sensors to take pictures at different radial positions of the detection components.
Referring to fig. 1, a multi-path microscopic imaging apparatus further includes a Z-axis adjustment assembly 150, where the Z-axis adjustment assembly is used to carry an image sensor, a beam splitter, and a lens assembly, and the Z-axis adjustment assembly adjusts a distance between a target object and the lens assembly.
The multipath microscopic imaging device also comprises a distance adjusting component, wherein the distance adjusting component carries an image sensor A or an image sensor B, and the distance adjusting component adjusts the distance between the image sensor and the lens component.
Referring to fig. 7, a multiple microscopy imaging device is shown with distance adjustment set 723 carried on Z-axis adjustment assembly 750, reference numeral 721 being an image sensor. The distance adjusting component is not carried on the Z-axis adjusting component, and the distance adjusting component is not carried on the Z-axis adjusting component, so that the movement amount brought by the Z-axis adjusting component is needed to be considered during independent adjustment.
A multipath microscopic imaging method divides a microscopic imaging light path into two paths through a spectroscope A, and the two paths of microscopic imaging light rays are imaged on an image sensor A or an image sensor B respectively.
A multipath microscopic imaging method; and (3) dividing one microscopic imaging light path into a third light path through the spectroscope B, and imaging the image sensor C on the third light path.
A multipath microscopic imaging method; and (3) dividing one microscopic imaging light path into a third light path through a spectroscope C, and arranging a marking light assembly on the third light path.
A multipath microscopic imaging method; it may be that the image sensors image different locations on the same focal plane, respectively.
The image sensors respectively image the same focal plane, and the image sensors pass through the filter plate, and the imaging corresponds to different wavelengths. Many measurements require measurement of the absorbance of different light waves, such as hemoglobin, fluorescence response, etc., and through different filters, the absorbance of different wavebands in a light field can be analyzed.
Referring to FIG. 8, a functional block diagram of a multi-channel microscopic imaging device, a digital camera C1, a digital camera C2, a lens assembly 850, an imaging light source 810, and a beam splitter 830; the imaging light source is used for illuminating a target 860, and the target is an imaging light path 870 from the lens assembly; the imaging light path is split by the spectroscope 830 and is divided into two light paths, and the digital camera C1 images on one light path 871; the digital camera C2 images on the other optical path 872.
The digital camera C2 is mounted on the guide rail 820 at an adjustable distance such that the focal plane S2 corresponding to the digital camera C2 is adjusted with respect to the focal plane S1 corresponding to the digital camera C1.
A multipath microscopic imaging method; the image sensors are arranged at different distances and respectively image different focal planes. By fixing the different distances, different detection objects, such as blood cell detection, can be detected, and white blood cells and red blood cells are basically fixed.
On the microscopic imaging light path, different lens groups are arranged, and different image sensors respectively image different focal planes.
The image sensor is provided with an adjustment distance means, which image the different focal planes.
A multipath microscopic imaging method; the microscopic imaging target is a detection chip, and the detection chip comprises a sample accommodating cavity; the image sensor images different detection targets in the sample accommodating cavity.
A multipath microscopic imaging method is characterized in that a detection chip holds a blood diluent sample, and an image sensor respectively performs focusing imaging on focal planes of white blood cells, red blood cells or platelets at the same time or at different times.
A multipath microscopic imaging method is characterized in that a detection chip contains a urine sample or a urine concentrated sample or a urine diluted sample, and an image sensor respectively performs focusing imaging on focal planes of a tubular type, a crystal, a cell and/or a pathogenic microorganism at the same time or at different times.
A multipath microscopic imaging method is characterized in that a detection chip contains fecal suspension, and an image sensor respectively performs focusing imaging on focal planes of parasitic ova, intestinal protozoa, starch particles, lipid drops, plant fibers, myofibers, cells and/or pathogenic microorganisms at the same time or at different times.
While the invention has been illustrated and described in terms of a preferred embodiment and several alternatives, the invention is not limited by the specific description in this specification. Other alternative or equivalent components may also be used in the practice of the present invention.
Claims (20)
1. The multipath microscopic imaging device is characterized by comprising an image sensor A, an image sensor B, a lens assembly, an imaging light source and a spectroscope A;
The imaging light source is used for irradiating the target object; the object-to-lens assembly is an imaging light path;
the imaging light path is split by the spectroscope A and is divided into two light paths, and the image sensor A images on one light path; the image sensor B images on the other light path;
The focal planes corresponding to the imaging of the image sensor A and the imaging of the image sensor B are different, and the different focal planes are imaged respectively;
The target object is a detection chip, and a sample accommodating cavity is formed in the detection chip;
the image sensor images different detection targets in the sample accommodating cavity respectively;
the distance adjusting component is used for bearing the image sensor A or the image sensor B and adjusting the distance between the image sensor and the lens component;
also comprises any one of the following technical characteristics:
TN1: the image sensor respectively performs focusing imaging on focal planes of white blood cells, red blood cells or platelets at the same time or at different times;
TN2: the image sensor respectively carries out focusing imaging on focal planes of the tube type, the crystal, the cell and/or the pathogenic microorganism at the same time or at different times;
TN3: the image sensor respectively performs focusing imaging on focal planes of parasitic ova, intestinal protozoa, starch particles, lipid droplets, plant fibers, myofibers, cells and/or pathogenic microorganisms simultaneously or not simultaneously.
2. The multi-channel microscopic imaging device of claim 1, further comprising a beam splitter B, an image sensor C;
and the spectroscope B divides one light path into a third light path, and the image sensor C images on the third light path.
3. The multi-channel microscopic imaging device of claim 1, further comprising a beam splitter C, a marker light assembly;
The spectroscope C splits one light path into marking light paths,
The marking light component emits marking light, a marking pattern is formed on the detection target through a marking light path, and the marking pattern is imaged on the image sensor A or the image sensor B through an imaging light path to form a marking image.
4. The multiplexing microscopic imaging device according to claim 1 or 2, wherein the image sensor is configured to image at different wavelengths through a filter.
5. The multiplexed microimaging device of claim 1, wherein the detection chip contains a blood diluent sample.
6. The multiplexing microscopic imaging device of claim 1, wherein the detection chip accommodates a urine sample or a urine concentrate sample or a urine dilute sample.
7. The multiplexed microimaging device of claim 1, wherein the detection chip contains a fecal suspension.
8. The multiplexed microimaging device of claim 1, further comprising a Z-axis adjustment assembly for carrying the image sensor, the beam splitter, and the lens assembly, the Z-axis adjustment assembly adjusting the distance between the target and the lens assembly.
9. The multiplexed microimaging apparatus of claim 8, wherein the distance adjustment assembly is carried on the Z-axis adjustment assembly or the distance adjustment assembly is not carried on the Z-axis adjustment assembly.
10. The multiplexed microimaging apparatus of claim 3, wherein the marker light assembly comprises a laser emitting device, the marker light being a laser; or the marking light assembly comprises an LED light source which emits red light, green light, blue light or yellow light; or the marking light assembly includes a light bulb.
11. The multi-channel microscopic imaging device of claim 3, wherein the marking light assembly comprises a marking light source, a perforated mask, the perforated mask comprising more than 3 holes;
the light emitted by the marking light source passes through the shading plate with holes to form more than 3 light spots on the surface of the target object;
The light spot is imaged on an image sensor; and judging the perpendicularity of the surface of the target object and the light path by judging whether the mark image is in the light spot or not or the definition.
12. A multipath microscopic imaging method is characterized in that a microscopic imaging light path is divided into two paths by a spectroscope A, and the two paths of microscopic imaging light rays are imaged on an image sensor A or an image sensor B respectively; the microscopic imaging target is a detection chip, and the detection chip comprises a sample accommodating cavity; the image sensor respectively images different detection targets in the sample accommodating cavity, and the image sensor comprises any one of the following technical characteristics,
The characteristic TC10 is arranged at different distances and images different focal planes respectively;
the characteristic TC20 is characterized in that different lens groups are arranged on the microscopic imaging optical path, and different image sensors respectively image different focal planes;
A feature TC30, the image sensor being provided with means for adjusting the distance, the image sensor being capable of imaging different focal planes;
also comprises any one of the following technical characteristics:
The characteristic TC40 is that the image sensor respectively performs focusing imaging on focal planes of white blood cells, red blood cells or platelets at the same time or different times;
the characteristic TC50 is that the image sensor respectively performs focusing imaging on focal planes of the tube type, the crystallization, the cells and/or the pathogenic microorganisms at the same time or at different times;
And the image sensor respectively performs focusing imaging on focal planes of parasitic ova, intestinal protozoa, starch particles, lipid droplets, plant fibers, myofibers, cells and/or pathogenic microorganisms at the same time or different times.
13. The multiplexed microimaging method of claim 12; the imaging device is characterized in that a path of microscopic imaging light path is separated into a third light path through a spectroscope B, and an image sensor C images on the third light path.
14. The multiplexed microimaging method of claim 12; the microscopic imaging optical system is characterized in that a path of microscopic imaging optical path is separated into a third optical path through a spectroscope C, and a marking optical assembly is arranged on the third optical path.
15. The multiplex microscopy imaging method according to claim 12 or 13; the imaging device is characterized in that the imaging sensor passes through the filter plate and corresponds to different wavelengths.
16. The multiplexed microimaging method of claim 12, wherein the detection chip contains a blood diluent sample.
17. The multiplexing method of claim 12, wherein the detection chip contains a urine sample or a urine concentrate sample or a urine dilute sample.
18. The multiplexed microimaging method of claim 12, wherein the detection chip contains a fecal suspension.
19. The multi-path microscopic imaging method according to claim 14, wherein the marking light assembly includes a laser emitting device, and the marking light is a laser; or the marking light assembly comprises an LED light source which emits red light, green light, blue light or yellow light; or the marking light assembly includes a light bulb.
20. The method of claim 14, wherein the marking light assembly comprises a marking light source, a perforated mask, the perforated mask comprising more than 3 holes;
the light emitted by the marking light source passes through the shading plate with holes to form more than 3 light spots on the surface of the target object; the light spot is imaged on an image sensor; and judging the perpendicularity of the surface of the target object and the light path by judging whether the mark image is in the light spot or not or the definition.
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| CN113176662A (en) * | 2021-05-31 | 2021-07-27 | 中国科学院苏州生物医学工程技术研究所 | Microscopic imaging device |
| CN115480385A (en) * | 2021-05-31 | 2022-12-16 | 博瑞生物医疗科技(深圳)有限公司 | Surgical microscope system and imaging method thereof |
| CN117147550A (en) * | 2023-09-06 | 2023-12-01 | 深圳安侣医学科技有限公司 | Device, chip and method for detecting and analyzing formed component with verticality adjustment |
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| US10876970B2 (en) * | 2016-04-12 | 2020-12-29 | The Board Of Regents Of The University Of Texas System | Light-sheet microscope with parallelized 3D image acquisition |
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| CN115480385A (en) * | 2021-05-31 | 2022-12-16 | 博瑞生物医疗科技(深圳)有限公司 | Surgical microscope system and imaging method thereof |
| CN117147550A (en) * | 2023-09-06 | 2023-12-01 | 深圳安侣医学科技有限公司 | Device, chip and method for detecting and analyzing formed component with verticality adjustment |
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