CN117679518A - Pharmaceutical composition for preventing and treating convalescence facial paralysis and application thereof - Google Patents

Pharmaceutical composition for preventing and treating convalescence facial paralysis and application thereof Download PDF

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Publication number
CN117679518A
CN117679518A CN202311159233.1A CN202311159233A CN117679518A CN 117679518 A CN117679518 A CN 117679518A CN 202311159233 A CN202311159233 A CN 202311159233A CN 117679518 A CN117679518 A CN 117679518A
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pharmaceutical composition
combination
facial paralysis
convalescence
treating
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陈琳
王宇
高文勇
李建军
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Beijing Darwin Cell Biotechnology Co ltd
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Beijing Darwin Cell Biotechnology Co ltd
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    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B18/00—Surgical instruments, devices or methods for transferring non-mechanical forms of energy to or from the body
    • A61B18/18—Surgical instruments, devices or methods for transferring non-mechanical forms of energy to or from the body by applying electromagnetic radiation, e.g. microwaves
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Abstract

The invention relates to a pharmaceutical composition for preventing and treating convalescence facial paralysis and application thereof, wherein the composition contains any one or combination of dehydration drugs, antiviral drugs, anti-inflammatory drugs, nerve repair drugs and/or drugs for improving blood circulation and Chinese medicinal preparations. The pharmaceutical composition combines radio frequency operation with nerve repair medicine and rehabilitation operation, is beneficial to promoting vegetative nerve and myelin and axon repair, has the advantages of synergistic effect, quick response, high effective rate, high cure rate, basically no side effect, basically no recurrence rate and the like, and remarkably improves the treatment prognosis and the quality of life of patients.

Description

Pharmaceutical composition for preventing and treating convalescence facial paralysis and application thereof
Technical Field
The invention belongs to the technical field of biological medicines, and particularly relates to a pharmaceutical composition for preventing and treating convalescence facial paralysis and application thereof.
Background
Facial paralysis (also called facial paralysis) is a common facial nerve disease (the ratio of which is 60% -75%), the incidence rate is 11.5-53.3 people/10 ten thousands of people, the recurrence rate is 2.6% -15.2%, and the patients are concentrated in men aged 20-40, and the main clinical manifestations of the facial paralysis include facial autonomous movement, reduced or lost expression function, facial nerve and facial expression musculature nutrition disorder and the like, and influence the appearance, personal dignity and social image of the patients, and serious patients even cause psychological disorder, depression anxiety and other symptoms of the patients. Clinically, facial paralysis is classified into an acute phase (within 15 days of onset), a recovery phase (within 16 days to 6 months of onset) and a sequelae phase (over 6 months of onset) according to the duration of onset of the patient.
The etiology of facial paralysis is not clear. Viral infections (e.g., latent herpes simplex virus infection and herpes zoster virus infection), reactivation after viral infection, and the like, are widely accepted as causative mechanisms of facial paralysis. Familial facial paralysis may be secondary to autoimmune diseases with inherited human leukocyte antigens. Abnormal facial nerve tube anatomy, stenosis of facial nerve tube, abrupt change of climate and temperature, etc. may become dangerous factors causing facial nerve paralysis. Different lesions of the facial nerve (including pre-ganglion lesions of the knee, lesions near the foramina of the stem, etc.) exhibit different clinical symptoms. If the facial paralysis patient is not treated timely and not recovered thoroughly, atrophy of paralyzed muscle, blepharospasm, joint movement and traction of affected side parts are often accompanied, and regenerated nerve fibers can grow to paths adjacent to other nerve fibers after the injury, so that other symptoms originally belonging to other nerve fiber effectors are dominated. At present, a medicine and a treatment scheme for effectively treating the convalescence facial paralysis are not available.
Patent applications (CN 2023100429139, PCT/CN2023/073566, CN2023100429143, PCT/CN 2023/073582) disclose technical content about a neural repair protein extract and a neural repair protein composition having repair efficacy, which are essential technical references and components of the present application.
Disclosure of Invention
The invention aims to provide an application of a pharmaceutical composition in preparing medicines for preventing and treating the facial paralysis in the recovery period, wherein the pharmaceutical composition for preventing and treating the facial paralysis in the recovery period contains any one or combination of a dehydration medicine, an antiviral medicine, an anti-inflammatory medicine, a nerve repair medicine and/or a blood circulation improving medicine and a traditional Chinese medicine preparation.
In a preferred embodiment of the present invention, the dehydration-based drug is selected from any one of mannitol, sorbitol, aescin sodium, 50% hypertonic glucose solution, or a combination thereof.
In a preferred embodiment of the present invention, the antiviral drug is selected from any one or combination of acyclovir, valacyclovir, dibazol, rhinoceros detoxification, virucide, fampicvir (faviravir), paxlovid, mo Nuola (Molnupiravir), oseltamivir, lei Midi vitamin, rendimivir (Remdesivir, GS-5734), indinavir, saquinavir, lopinavir, ritonavir (Ritonavir), atazanavir, darunavir, telanavir, fosamprenavir, enzaton Weipu ritodunder, abacavir, eticonvir, ma Liba, raltegafvir, ribavirin, rimantadine, oseltamivir, zanavir, peramivir, ganciclovir, baloflavir Sha Wei (baenavir), and fevernivir.
In a preferred embodiment of the present invention, the anti-inflammatory drug is selected from any one of dexamethasone, methylprednisolone, prednisone, methylprednisolone (methylprednisolone), cortisone, hydrocortisone, prednisone, prednisolone, or a combination thereof.
In a preferred embodiment of the present invention, the nerve repair drug and/or the blood circulation improving drug is selected from any one or a combination of murine nerve growth factor, monosialoganglioside (GM 1), cerebroside carnosine, mecobalamin, adenosyl cobalamin, compound vitamin B, butylphthalide, cinepazide maleate, edaravone dexkaempferol, citicoline, sodium citicoline, ganglioside, oxiracetam, piracetam, aniracetam, nerve growth factor, citicoline, neurotoxicam, oryzanol, vitamin B1, vitamin B6, vitamin B12, vitamin C, vitamin E, compound brain peptide ganglioside, brain protein, and nervonic acid.
In the preferred technical scheme of the invention, the traditional Chinese medicine preparation is selected from any one or combination of traditional Chinese medicine ointment prepared from fresh ginger, ginseng, lotus root, chinese yam, liquorice, chicory, rose and angelica, or modified Chinese medicine composition of facial paralysis pill, ginseng reconstruction pill, ginkgo leaf or ginkgo leaf extract, kudzu vine root decoction, modified Qianzheng powder or modified Chinese medicine composition thereof, large gentian root decoction or modified Chinese gentian root decoction, modified snakegourd fruit and safflower decoction or modified Chinese medicine composition thereof, phlegm-eliminating decoction, modified Chinese medicine composition of the powder or modified Chinese medicine composition thereof, yang-tonifying five-decoction, modified Qianzheng powder or modified Chinese medicine composition thereof, damp-hurting pain-relieving ointment and musk tiger bone ointment.
In a preferred technical scheme of the invention, the pharmaceutical composition for preventing and treating the convalescence facial paralysis contains any one or combination of mannitol, sodium aescinate, acyclovir, valacyclovir, dibazol, dexamethasone, prednisone, citicoline sodium, ginkgo leaf, murine nerve growth factor, mecobalamin and adenosylcobalamin.
In a preferred technical scheme of the invention, the pharmaceutical composition for preventing and treating the convalescence facial paralysis contains any one or combination of mannitol, sodium aescinate, acyclovir, valacyclovir, dibazol, dexamethasone, prednisone, citicoline sodium, ginkgo leaf, murine nerve growth factor, mecobalamin and adenosylcobalamin.
In a preferred technical scheme of the invention, the pharmaceutical composition for preventing and treating the convalescence facial paralysis consists of a pharmaceutical composition for single oral administration and a pharmaceutical composition for single acupoint injection.
In the preferred technical scheme of the invention, the pharmaceutical composition for single oral administration contains 20-40mg of aescin sodium, 10-30mg of dibazol, 0.5-1.5mg of mecobalamin, 0.5-1.5mg of adenosylcobalamin, 0.2-0.6g of ginkgo leaf and 3-6g of ginseng re-made pills.
In a preferred embodiment of the present invention, the oral pharmaceutical composition is administered according to the following schedule:
(1) Oral administration of aescin sodium 20 mg/time, 2 times/day, and administration for 1 month;
(2) 10 mg/time, 3 times/day of oral dibazol for 1 month;
(3) Oral mecobalamin 0.5 mg/time and adenosylcobalamin 0.5 mg/time, 3 times/day for 1 month;
(4) Orally administering ginkgo leaf 0.2 g/time, 3 times/day for 1 month;
(5) The oral administration of the ginseng regeneration pill is 3 g/time, 2 times/day, and 1 month.
In a preferred embodiment of the present invention, the pharmaceutical composition for oral administration is selected from any one of simultaneous administration and sequential administration or a combination thereof.
In the preferred technical scheme of the invention, the pharmaceutical composition for single acupoint injection contains 30ug of mouse nerve growth factor, 0.5mg of mecobalamin and vitamin B 1 200mg, or any combination thereof.
In the preferred technical scheme of the invention, the pharmaceutical composition for single acupoint injection consists of 30ug-90ug of mouse nerve growth factor, 0.5mg-1.0mg of mecobalamin, 0.5mg-1.0mg of adenosylcobalamin, 2mg-5mg of dexamethasone and 1ml of lidocaine hydrochloride, wherein the concentration of the lidocaine hydrochloride is selected from any one of 0.8%, 1%, 1.5% and 2%.
In a preferred technical scheme of the invention, the pharmaceutical composition for single acupoint injection consists of 30ug of mouse nerve growth factor, 0.5mg of mecobalamin, 0.5mg of adenosylcobalamin, 2mg of dexamethasone and 1ml of lidocaine hydrochloride, wherein the concentration of the lidocaine hydrochloride is selected from any one of 0.8%, 1%, 1.5% and 2%.
In a preferred technical scheme of the invention, the pharmaceutical composition for single acupoint injection consists of 30ug of mouse nerve growth factor, 0.5mg of mecobalamin, 1.0mg of adenosylcobalamin, 5mg of dexamethasone and 1ml of lidocaine hydrochloride, wherein the concentration of the lidocaine hydrochloride is selected from any one of 0.8%, 1%, 1.5% and 2%.
In a preferred technical scheme of the invention, the pharmaceutical composition for single acupoint injection consists of 90ug of mouse nerve growth factor, 1.0mg of mecobalamin, 0.5mg of adenosylcobalamin, 5mg of dexamethasone and 1ml of lidocaine hydrochloride, wherein the concentration of the lidocaine hydrochloride is selected from any one of 0.8%, 1%, 1.5% and 2%.
In a preferred technical scheme of the invention, the pharmaceutical composition for single acupoint injection consists of 60ug of mouse nerve growth factor, 1.0mg of mecobalamin, 0.5mg of adenosylcobalamin, 3mg of dexamethasone and 1ml of lidocaine hydrochloride, wherein the concentration of the lidocaine hydrochloride is selected from any one of 0.8%, 1%, 1.5% and 2%.
In a preferred embodiment of the present invention, the pharmaceutical composition for single acupoint injection optionally contains 100-300mg of any one or combination of a neural repair cell protein extract and/or a neural repair protein composition having a repair effect.
In the preferred technical scheme of the invention, the pharmaceutical composition for single acupoint injection is clinically matched.
In the preferred technical scheme of the invention, the yang white acupoint, the temple, the Sibai acupoint, the Yingxiang acupoint, the Juliao acupoint, the Dicang acupoint and the Jiache acupoint of the affected side face part are selected for acupoint injection.
In the preferred technical scheme of the invention, the medicine composition for single acupoint injection is injected once per acupoint every day, and 7 days is a treatment course.
In the preferred technical scheme of the invention, each acupoint injection treatment is carried out for 2-6 treatment courses, preferably 4-5 treatment courses.
In a preferred embodiment of the present invention, the pharmaceutical composition for preventing and treating convalescence facial paralysis is optionally used in combination with any one of radio frequency surgery, rehabilitation training or a combination thereof.
In the preferable technical scheme of the invention, the radio frequency operation is based on long-time temperature control high-voltage variable-frequency pulse radio frequency for 800 seconds to 1400 seconds under the conditions of 41 ℃ to 42 ℃, 90V to 140V and 2Hz to 6 Hz.
In the preferable technical scheme of the invention, the radio frequency operation is based on long-time temperature control high-voltage variable-frequency pulse radio frequency 960 seconds-1200 seconds under the conditions of 41-42 ℃, 100-120V and 3-5 Hz.
In a preferred technical scheme of the invention, the rehabilitation training operation is a facial paralysis rehabilitation training operation (copyright registration number: national registration number-2022-I-10250228 and copyright registration number: national registration number-2022-F-10250229) which is originally created by the inventor.
In a preferred technical scheme of the invention, the rehabilitation exercise training is selected from any one or combination of a first set of rehabilitation exercise training and a second set of rehabilitation exercise training.
In the preferred technical scheme of the invention, the first set of rehabilitation exercise training comprises a mirror; secondly, slowly closing the eyes, opening the eyes for 5 seconds after closing the eyes for 5 seconds, and simultaneously controlling the continuous lifting action of the mouth angle without occurrence, correcting deviation and correcting continuous movement; the first three groups of rehabilitation exercises were trained daily, 30 times per group.
In the preferred technical scheme of the invention, the second set of rehabilitation exercise training comprises a mirror; secondly, eyes are opened, and silent nerve branches are kept as much as possible; the third is perioral puckering, the teeth and the cheeks; the first three groups of rehabilitation exercises were trained daily, 10 times per group.
In the preferred technical scheme of the invention, the treatment scheme for preventing and treating the convalescence facial paralysis is pulse radio frequency operation, postoperative oral administration and acupoint injection administration, and intraoperative and postoperative rehabilitation exercise training.
In a preferred embodiment of the present invention, the facial paralysis is selected from any one of facial spasm, facial paralysis/joint movement, trigeminal neuralgia, glossopharyngeal neuralgia, facial neuritis, peripheral facial paralysis and repair of ocular spasm or a combination thereof.
The invention aims to provide a pharmaceutical composition for preventing and treating convalescence facial paralysis, which contains any one or combination of dehydration drugs, antiviral drugs, anti-inflammatory drugs, nerve repair drugs and/or drugs for improving blood circulation and traditional Chinese medicine preparations.
In a preferred embodiment of the present invention, the dehydration-based drug is selected from any one of mannitol, sorbitol, aescin sodium, 50% hypertonic glucose solution, or a combination thereof.
In a preferred embodiment of the present invention, the antiviral drug is selected from any one or combination of acyclovir, valacyclovir, dibazol, rhinoceros detoxification, virucide, fampicvir (faviravir), paxlovid, mo Nuola (Molnupiravir), oseltamivir, lei Midi vitamin, rendimivir (Remdesivir, GS-5734), indinavir, saquinavir, lopinavir, ritonavir (Ritonavir), atazanavir, darunavir, telanavir, fosamprenavir, enzaton Weipu ritodunder, abacavir, eticonvir, ma Liba, raltegafvir, ribavirin, rimantadine, oseltamivir, zanavir, peramivir, ganciclovir, baloflavir Sha Wei (baenavir), and fevernivir.
In a preferred embodiment of the present invention, the anti-inflammatory drug is selected from any one of dexamethasone, methylprednisolone, prednisone, methylprednisolone (methylprednisolone), cortisone, hydrocortisone, prednisone, prednisolone, or a combination thereof.
In a preferred embodiment of the present invention, the nerve repair drug and/or the blood circulation improving drug is selected from any one or a combination of murine nerve growth factor, monosialoganglioside (GM 1), cerebroside carnosine, mecobalamin, adenosyl cobalamin, compound vitamin B, butylphthalide, cinepazide maleate, edaravone dexkaempferol, citicoline, sodium citicoline, ganglioside, oxiracetam, piracetam, aniracetam, nerve growth factor, citicoline, neurotoxicam, oryzanol, vitamin B1, vitamin B6, vitamin B12, vitamin C, vitamin E, compound brain peptide ganglioside, brain protein, and nervonic acid.
In the preferred technical scheme of the invention, the traditional Chinese medicine preparation is selected from any one or combination of traditional Chinese medicine ointment prepared from fresh ginger, ginseng, lotus root, chinese yam, liquorice, chicory, rose and angelica, or modified Chinese medicine composition of facial paralysis pill, ginseng reconstruction pill, ginkgo leaf or ginkgo leaf extract, kudzu vine root decoction, modified Qianzheng powder or modified Chinese medicine composition thereof, large gentian root decoction or modified Chinese gentian root decoction, modified snakegourd fruit and safflower decoction or modified Chinese medicine composition thereof, phlegm-eliminating decoction, modified Chinese medicine composition of the powder or modified Chinese medicine composition thereof, yang-tonifying five-decoction, modified Qianzheng powder or modified Chinese medicine composition thereof, damp-hurting pain-relieving ointment and musk tiger bone ointment.
In a preferred technical scheme of the invention, the pharmaceutical composition for preventing and treating the convalescence facial paralysis contains any one or combination of mannitol, sodium aescinate, acyclovir, valacyclovir, dibazol, dexamethasone, prednisone, citicoline sodium, ginkgo leaf, murine nerve growth factor, mecobalamin and adenosylcobalamin.
In a preferred technical scheme of the invention, the pharmaceutical composition for preventing and treating the convalescence facial paralysis contains any one or combination of mannitol, sodium aescinate, acyclovir, valacyclovir, dibazol, dexamethasone, prednisone, citicoline sodium, ginkgo leaf, murine nerve growth factor, mecobalamin and adenosylcobalamin.
In a preferred technical scheme of the invention, the pharmaceutical composition for preventing and treating the convalescence facial paralysis consists of a pharmaceutical composition for single oral administration and a pharmaceutical composition for single acupoint injection.
In the preferred technical scheme of the invention, the pharmaceutical composition for single oral administration contains 20-40mg of aescin sodium, 10-30mg of dibazol, 0.5-1.5mg of mecobalamin, 0.5-1.5mg of adenosylcobalamin, 0.2-0.6g of ginkgo leaf and 3-6g of ginseng re-made pills.
In a preferred embodiment of the present invention, the oral pharmaceutical composition is administered according to the following schedule:
(1) Oral administration of aescin sodium 20 mg/time, 2 times/day, and administration for 1 month;
(2) 10 mg/time, 3 times/day of oral dibazol for 1 month;
(3) Oral mecobalamin 0.5 mg/time and adenosylcobalamin 0.5 mg/time, 3 times/day for 1 month;
(4) Orally administering ginkgo leaf 0.2 g/time, 3 times/day for 1 month;
(5) The oral administration of the ginseng regeneration pill is 3 g/time, 2 times/day, and 1 month.
In a preferred embodiment of the present invention, the pharmaceutical composition for oral administration is selected from any one of simultaneous administration and sequential administration or a combination thereof.
In the preferred technical scheme of the invention, the pharmaceutical composition for single acupoint injection contains 30ug of mouse nerve growth factor, 0.5mg of mecobalamin and vitamin B 1 200mg, or any combination thereof.
In the preferred technical scheme of the invention, the pharmaceutical composition for single acupoint injection consists of 30ug-90ug of mouse nerve growth factor, 0.5mg-1.0mg of mecobalamin, 0.5mg-1.0mg of adenosylcobalamin, 2mg-5mg of dexamethasone and 1ml of lidocaine hydrochloride, wherein the concentration of the lidocaine hydrochloride is selected from any one of 0.8%, 1%, 1.5% and 2%.
In a preferred technical scheme of the invention, the pharmaceutical composition for single acupoint injection consists of 30ug of mouse nerve growth factor, 0.5mg of mecobalamin, 0.5mg of adenosylcobalamin, 2mg of dexamethasone and 1ml of lidocaine hydrochloride, wherein the concentration of the lidocaine hydrochloride is selected from any one of 0.8%, 1%, 1.5% and 2%.
In a preferred technical scheme of the invention, the pharmaceutical composition for single acupoint injection consists of 30ug of mouse nerve growth factor, 0.5mg of mecobalamin, 1.0mg of adenosylcobalamin, 5mg of dexamethasone and 1ml of lidocaine hydrochloride, wherein the concentration of the lidocaine hydrochloride is selected from any one of 0.8%, 1%, 1.5% and 2%.
In a preferred technical scheme of the invention, the pharmaceutical composition for single acupoint injection consists of 90ug of mouse nerve growth factor, 1.0mg of mecobalamin, 0.5mg of adenosylcobalamin, 5mg of dexamethasone and 1ml of lidocaine hydrochloride, wherein the concentration of the lidocaine hydrochloride is selected from any one of 0.8%, 1%, 1.5% and 2%.
In a preferred technical scheme of the invention, the pharmaceutical composition for single acupoint injection consists of 60ug of mouse nerve growth factor, 1.0mg of mecobalamin, 0.5mg of adenosylcobalamin, 3mg of dexamethasone and 1ml of lidocaine hydrochloride, wherein the concentration of the lidocaine hydrochloride is selected from any one of 0.8%, 1%, 1.5% and 2%.
In a preferred embodiment of the present invention, the pharmaceutical composition for single acupoint injection optionally contains 100-300mg of any one or combination of a neural repair cell protein extract and/or a neural repair protein composition having a repair effect.
In the preferred technical scheme of the invention, the pharmaceutical composition for single acupoint injection is clinically matched.
In the preferred technical scheme of the invention, the yang white acupoint, the temple, the Sibai acupoint, the Yingxiang acupoint, the Juliao acupoint, the Dicang acupoint and the Jiache acupoint of the affected side face part are selected for acupoint injection.
In the preferred technical scheme of the invention, the medicine composition for single acupoint injection is injected once per acupoint every day, and 7 days is a treatment course.
In the preferred technical scheme of the invention, each acupoint injection treatment is carried out for 2-6 treatment courses, preferably 4-5 treatment courses.
In a preferred embodiment of the present invention, the pharmaceutical composition for preventing and treating convalescence facial paralysis is optionally used in combination with any one of radio frequency surgery, rehabilitation training or a combination thereof.
In the preferable technical scheme of the invention, the radio frequency operation is based on long-time temperature control high-voltage variable-frequency pulse radio frequency for 800 seconds to 1400 seconds under the conditions of 41 ℃ to 42 ℃, 90V to 140V and 2Hz to 6 Hz.
In the preferable technical scheme of the invention, the radio frequency operation is based on long-time temperature control high-voltage variable-frequency pulse radio frequency 960 seconds-1200 seconds under the conditions of 41-42 ℃, 100-120V and 3-5 Hz.
In a preferred technical scheme of the invention, the rehabilitation training operation is a facial paralysis rehabilitation training operation (copyright registration number: national registration number-2022-I-10250228 and copyright registration number: national registration number-2022-F-10250229) which is originally created by the inventor.
In a preferred technical scheme of the invention, the rehabilitation exercise training is selected from any one or combination of a first set of rehabilitation exercise training and a second set of rehabilitation exercise training.
In the preferred technical scheme of the invention, the first set of rehabilitation exercise training comprises a mirror; secondly, slowly closing the eyes, opening the eyes for 5 seconds after closing the eyes for 5 seconds, and simultaneously controlling the continuous lifting action of the mouth angle without occurrence, correcting deviation and correcting continuous movement; the first three groups of rehabilitation exercises were trained daily, 30 times per group.
In the preferred technical scheme of the invention, the second set of rehabilitation exercise training comprises a mirror; secondly, eyes are opened, and silent nerve branches are kept as much as possible; the third is perioral puckering, the teeth and the cheeks; the first three groups of rehabilitation exercises were trained daily, 10 times per group.
In the preferred technical scheme of the invention, the treatment scheme for preventing and treating the convalescence facial paralysis is pulse radio frequency operation, postoperative oral administration and acupoint injection administration, and intraoperative and postoperative rehabilitation exercise training.
In a preferred embodiment of the present invention, the facial paralysis is selected from any one of facial spasm, facial paralysis/joint movement, trigeminal neuralgia, glossopharyngeal neuralgia, facial neuritis, peripheral facial paralysis and repair of ocular spasm or a combination thereof.
It is another object of the present invention to provide a therapeutic regimen for preventing and treating convalescence facial paralysis comprising a pharmaceutical composition for preventing and treating convalescence facial paralysis, optionally in combination with any one of radio frequency surgery, rehabilitation training or a combination thereof.
In a preferred technical scheme of the invention, the pharmaceutical composition for preventing and treating the convalescence facial paralysis contains any one or combination of dehydration drugs, antiviral drugs, anti-inflammatory drugs, nerve repair drugs and/or drugs for improving blood circulation and Chinese medicinal preparations.
In a preferred embodiment of the present invention, the dehydration-based drug is selected from any one of mannitol, sorbitol, aescin sodium, 50% hypertonic glucose solution, or a combination thereof.
In a preferred embodiment of the present invention, the antiviral drug is selected from any one or combination of acyclovir, valacyclovir, dibazol, rhinoceros detoxification, virucide, fampicvir (faviravir), paxlovid, mo Nuola (Molnupiravir), oseltamivir, lei Midi vitamin, rendimivir (Remdesivir, GS-5734), indinavir, saquinavir, lopinavir, ritonavir (Ritonavir), atazanavir, darunavir, telanavir, fosamprenavir, enzaton Weipu ritodunder, abacavir, eticonvir, ma Liba, raltegafvir, ribavirin, rimantadine, oseltamivir, zanavir, peramivir, ganciclovir, baloflavir Sha Wei (baenavir), and fevernivir.
In a preferred embodiment of the present invention, the anti-inflammatory drug is selected from any one of dexamethasone, methylprednisolone, prednisone, methylprednisolone (methylprednisolone), cortisone, hydrocortisone, prednisone, prednisolone, or a combination thereof.
In a preferred embodiment of the present invention, the nerve repair drug and/or the blood circulation improving drug is selected from any one or a combination of murine nerve growth factor, monosialoganglioside (GM 1), cerebroside carnosine, mecobalamin, adenosyl cobalamin, compound vitamin B, butylphthalide, cinepazide maleate, edaravone dexkaempferol, citicoline, sodium citicoline, ganglioside, oxiracetam, piracetam, aniracetam, nerve growth factor, citicoline, neurotoxicam, oryzanol, vitamin B1, vitamin B6, vitamin B12, vitamin C, vitamin E, compound brain peptide ganglioside, brain protein, and nervonic acid.
In the preferred technical scheme of the invention, the traditional Chinese medicine preparation is selected from any one or combination of traditional Chinese medicine ointment prepared from fresh ginger, ginseng, lotus root, chinese yam, liquorice, chicory, rose and angelica, or modified Chinese medicine composition of facial paralysis pill, ginseng reconstruction pill, ginkgo leaf or ginkgo leaf extract, kudzu vine root decoction, modified Qianzheng powder or modified Chinese medicine composition thereof, large gentian root decoction or modified Chinese gentian root decoction, modified snakegourd fruit and safflower decoction or modified Chinese medicine composition thereof, phlegm-eliminating decoction, modified Chinese medicine composition of the powder or modified Chinese medicine composition thereof, yang-tonifying five-decoction, modified Qianzheng powder or modified Chinese medicine composition thereof, damp-hurting pain-relieving ointment and musk tiger bone ointment.
In a preferred technical scheme of the invention, the pharmaceutical composition for preventing and treating the convalescence facial paralysis contains any one or combination of mannitol, sodium aescinate, acyclovir, valacyclovir, dibazol, dexamethasone, prednisone, citicoline sodium, ginkgo leaf, murine nerve growth factor, mecobalamin and adenosylcobalamin.
In a preferred technical scheme of the invention, the pharmaceutical composition for preventing and treating the convalescence facial paralysis contains any one or combination of mannitol, sodium aescinate, acyclovir, valacyclovir, dibazol, dexamethasone, prednisone, citicoline sodium, ginkgo leaf, murine nerve growth factor, mecobalamin and adenosylcobalamin.
In a preferred technical scheme of the invention, the pharmaceutical composition for preventing and treating the convalescence facial paralysis consists of a pharmaceutical composition for single oral administration and a pharmaceutical composition for single acupoint injection.
In the preferred technical scheme of the invention, the pharmaceutical composition for single oral administration contains 20-40mg of aescin sodium, 10-30mg of dibazol, 0.5-1.5mg of mecobalamin, 0.5-1.5mg of adenosylcobalamin, 0.2-0.6g of ginkgo leaf and 3-6g of ginseng re-made pills.
In a preferred embodiment of the present invention, the oral pharmaceutical composition is administered according to the following schedule:
(1) Oral administration of aescin sodium 20 mg/time, 2 times/day, and administration for 1 month;
(2) 10 mg/time, 3 times/day of oral dibazol for 1 month;
(3) Oral mecobalamin 0.5 mg/time and adenosylcobalamin 0.5 mg/time, 3 times/day for 1 month;
(4) Orally administering ginkgo leaf 0.2 g/time, 3 times/day for 1 month;
(5) The oral administration of the ginseng regeneration pill is 3 g/time, 2 times/day, and 1 month.
In a preferred embodiment of the present invention, the pharmaceutical composition for oral administration is selected from any one of simultaneous administration and sequential administration or a combination thereof.
In the preferred technical scheme of the invention, the pharmaceutical composition for single acupoint injection contains 30ug of mouse nerve growth factor, 0.5mg of mecobalamin and vitamin B 1 200mg, or any combination thereof.
In the preferred technical scheme of the invention, the pharmaceutical composition for single acupoint injection consists of 30ug-90ug of mouse nerve growth factor, 0.5mg-1.0mg of mecobalamin, 0.5mg-1.0mg of adenosylcobalamin, 2mg-5mg of dexamethasone and 1ml of lidocaine hydrochloride, wherein the concentration of the lidocaine hydrochloride is selected from any one of 0.8%, 1%, 1.5% and 2%.
In a preferred technical scheme of the invention, the pharmaceutical composition for single acupoint injection consists of 30ug of mouse nerve growth factor, 0.5mg of mecobalamin, 0.5mg of adenosylcobalamin, 2mg of dexamethasone and 1ml of lidocaine hydrochloride, wherein the concentration of the lidocaine hydrochloride is selected from any one of 0.8%, 1%, 1.5% and 2%.
In a preferred technical scheme of the invention, the pharmaceutical composition for single acupoint injection consists of 30ug of mouse nerve growth factor, 0.5mg of mecobalamin, 1.0mg of adenosylcobalamin, 5mg of dexamethasone and 1ml of lidocaine hydrochloride, wherein the concentration of the lidocaine hydrochloride is selected from any one of 0.8%, 1%, 1.5% and 2%.
In a preferred technical scheme of the invention, the pharmaceutical composition for single acupoint injection consists of 90ug of mouse nerve growth factor, 1.0mg of mecobalamin, 0.5mg of adenosylcobalamin, 5mg of dexamethasone and 1ml of lidocaine hydrochloride, wherein the concentration of the lidocaine hydrochloride is selected from any one of 0.8%, 1%, 1.5% and 2%.
In a preferred technical scheme of the invention, the pharmaceutical composition for single acupoint injection consists of 60ug of mouse nerve growth factor, 1.0mg of mecobalamin, 0.5mg of adenosylcobalamin, 3mg of dexamethasone and 1ml of lidocaine hydrochloride, wherein the concentration of the lidocaine hydrochloride is selected from any one of 0.8%, 1%, 1.5% and 2%.
In a preferred embodiment of the present invention, the pharmaceutical composition for single acupoint injection optionally contains 100-300mg of any one or combination of a neural repair cell protein extract and/or a neural repair protein composition having a repair effect.
In the preferred technical scheme of the invention, the pharmaceutical composition for single acupoint injection is clinically matched.
In the preferred technical scheme of the invention, the yang white acupoint, the temple, the Sibai acupoint, the Yingxiang acupoint, the Juliao acupoint, the Dicang acupoint and the Jiache acupoint of the affected side face part are selected for acupoint injection.
In the preferred technical scheme of the invention, the medicine composition for single acupoint injection is injected once per acupoint every day, and 7 days is a treatment course.
In the preferred technical scheme of the invention, each acupoint injection treatment is carried out for 2-6 treatment courses, preferably 4-5 treatment courses.
In the preferable technical scheme of the invention, the radio frequency operation is based on long-time temperature control high-voltage variable-frequency pulse radio frequency for 800 seconds to 1400 seconds under the conditions of 41 ℃ to 42 ℃, 90V to 140V and 2Hz to 6 Hz.
In the preferable technical scheme of the invention, the radio frequency operation is based on long-time temperature control high-voltage variable-frequency pulse radio frequency 960 seconds-1200 seconds under the conditions of 41-42 ℃, 100-120V and 3-5 Hz.
In a preferred technical scheme of the invention, the rehabilitation training operation is a facial paralysis rehabilitation training operation (copyright registration number: national registration number-2022-I-10250228 and copyright registration number: national registration number-2022-F-10250229) which is originally created by the inventor.
In a preferred technical scheme of the invention, the rehabilitation exercise training is selected from any one or combination of a first set of rehabilitation exercise training and a second set of rehabilitation exercise training.
In the preferred technical scheme of the invention, the first set of rehabilitation exercise training comprises a mirror; secondly, slowly closing the eyes, opening the eyes for 5 seconds after closing the eyes for 5 seconds, and simultaneously controlling the continuous lifting action of the mouth angle without occurrence, correcting deviation and correcting continuous movement; the first three groups of rehabilitation exercises were trained daily, 30 times per group.
In the preferred technical scheme of the invention, the second set of rehabilitation exercise training comprises a mirror; secondly, eyes are opened, and silent nerve branches are kept as much as possible; the third is perioral puckering, the teeth and the cheeks; the first three groups of rehabilitation exercises were trained daily, 10 times per group.
In the preferred technical scheme of the invention, the treatment scheme for preventing and treating the convalescence facial paralysis is pulse radio frequency operation, postoperative oral administration and acupoint injection administration, and intraoperative and postoperative rehabilitation exercise training.
In a preferred embodiment of the present invention, the facial paralysis is selected from any one of facial spasm, facial paralysis/joint movement, trigeminal neuralgia, glossopharyngeal neuralgia, facial neuritis, peripheral facial paralysis and repair of ocular spasm or a combination thereof.
For clarity of presentation of the present invention, the neural repair cell protein extract or neural repair cell protein composition with repair efficacy according to the present invention was prepared with reference to patent applications (CN 2023100429139, PCT/CN2023/073566, CN2023100429143, PCT/CN 2023/073582).
In a preferred technical scheme of the invention, the preparation method of the nerve repair cell protein extract with the nerve repair effect comprises the following steps:
s-1 the density was set at 5.0X10 6 personal/mL-5.0X10 7 The mesenchymal stem cells of each/mL are placed in a culture medium containing DMEM/F12-50%, RPMI1640 40-50%, bovine Serum Albumin (BSA) 0.1-2%, epidermal Growth Factor (EGF) 1-15ug/mL, fibroblast Growth Factor (FGF) 1-15ug/mL, insulin transferrin 1-15ug/mL, compound amino acid (18 AA) 0.01-0.1% and 2-10 mu mol/L stressor, and then placed in a medium containing 37.0+/-0.5 ℃ and 5% +/-1.0% CO 2 After 2h-6h of culture under the condition, separating, washing and collecting cells, wherein the stressor is selected from any one of compounds 1-16 or a combination thereof;
s-2: will receiveThe cell concentration was 5.0X10 according to the density 6 personal/mL-5.0X10 7 Dispersing the cells/mL in a solvent, and then carrying out ultrasonic treatment at the temperature of 2-8 ℃ to prepare a cell lysate, wherein the solvent is selected from any one or a combination of physiological saline, 5% glucose solution, phosphate Buffer (PBS), TBPS buffer, TBST buffer and Tris buffer;
s-3, separating the cell lysate prepared in the step S-2, and filtering the obtained separating liquid with 0.45um and 0.22um filter membranes in sequence to obtain the cell lysate.
In the preferred technical scheme of the invention, the culture medium in the step S-1 contains stress substances of DMEM/F12-45%, RPMI1640 42-45%, bovine Serum Albumin (BSA) 0.5-1.5%, epidermal Growth Factor (EGF) 5-10ug/mL, fibroblast Growth Factor (FGF) 5-10ug/mL, insulin transferrin 5-10ug/mL, compound amino acid (18 AA) 0.02-0.05% and 3-8 mu mol/L.
In a preferred technical scheme of the invention, the culture medium in the step S-1 contains DMEM/F12, RPMI1640 45, bovine Serum Albumin (BSA) 0.5, epidermal Growth Factor (EGF) 10ug/mL, fibroblast Growth Factor (FGF) 10ug/mL, insulin transferrin 10ug/mL, compound amino acid (18 AA) 0.05% and stressors of 4-6 mu mol/L.
In a preferred embodiment of the present invention, the mesenchymal stem cells of step S-1 have a density of 8.0X10 6 -2.0×10 7 Each mL is preferably 8.0X10 6 -1.0×10 7 And each mL.
In a preferred embodiment of the invention, the mesenchymal stem cells of step S-1 are cultured in the medium for 3h to 5h, preferably 3.5h to 4.5h.
In a preferred embodiment of the present invention, the solvent used for washing the cells in step S-1 is selected from any one or a combination of physiological saline, 5% dextrose solution, phosphate Buffer (PBS), TBPS buffer, TBST buffer, tris buffer, and the like, and the number of times of washing the cells is 2 to 5 times, preferably 3 to 4 times.
In a preferred embodiment of the present invention, the separation in step S-1 is selected from any one of centrifugation and filtration or a combination thereof, wherein the centrifugation conditions are 1000-2000rpm for 3-15min, preferably 1200-1500 rpm for 5-10min.
In the preferred technical scheme of the invention, the ultrasonic conditions of the step S-2 are as follows: working for 3s at 2-8 ℃ under the conditions of 25kHZ and 360W and then spacing for 1s, and carrying out ultrasonic treatment for 1-5min.
In a preferred embodiment of the present invention, the separation in step S-3 is selected from any one or a combination of centrifugation at 2000-8000rpm for 10-30min, multistage centrifugation, multistage filtration, preferably 3000-7000rpm for 15-25min.
In a preferred embodiment of the present invention, the multistage centrifugation in step S-3 is carried out sequentially at 3000-4000rpm for 3-5min, at 5000-6000rpm for 3-5min, and at 7000rpm for 5-8min.
In a preferred technical scheme of the invention, the pore diameter of the multi-stage filtration membrane is selected from any one of 80um, 50um, 30um, 10um and 5 um.
In a preferred embodiment of the present invention, the cellular protein extract obtained in step S-3 is frozen, preferably at-40℃to-20 ℃.
In the preferred technical scheme of the invention, the cellular protein extract prepared in the step S-3 is subjected to enzymolysis by adopting any one of nuclease or totipotent nuclease and then is subjected to separation and purification.
In a preferred embodiment of the present invention, the mesenchymal stem cells are cultured or primary mesenchymal stem cells are cultured by a method of culturing in the art.
In a preferred embodiment of the present invention, the culture of the mesenchymal stem cells comprises the following steps: the primary mesenchymal stem cells are prepared according to the initial density of 5.0x10 5 -5.0×10 6 Adding the mixture/ml into a subculture medium, and placing the subculture medium at 37.0deg.C+ -0.5deg.C and 5% + -1.0% CO 2 Culturing under the condition for 10-15 days, observing yellowing of the subculture medium every 2-3 days, and half-changing the subculture medium, wherein the subculture medium contains 10% FBS, 100U/ml penicillin and 100ug/ml streptomycin DMEM/F12 medium.
In a preferred embodiment of the present invention, the culture of primary mesenchymal stem cells comprises the steps of:
1) Cleaning umbilical cord, sterilizing, dissecting tissue, collecting the tissue of HUALONG gel layer, and cutting into 3m piecesm 3 Is centrifuged, washed, tissue pieces are collected, placed in DMEM/F12 medium containing 10% fetal bovine serum FBS, 100ug/ml penicillin, 100ug/ml streptomycin, and placed in 37.0.+ -. 0.5 ℃ and 5%.+ -. 1.0% CO 2 Culturing under the condition that the culture medium is half replaced every 2-3 days, and culturing until the tissue blocks climb out of the cells;
2) Shaking, collecting low-layer cells, washing with PBS, adding 0.25% trypsin, digesting for 2min-3min, adding equal volume of trypsin stopping solution, stopping digestion, gently blowing with a sucker, centrifuging at 1200-1500rpm/min for 5-8min, and collecting cells.
Unless otherwise indicated, when the invention relates to a percentage between liquids, the percentages are volume/volume percentages; the invention relates to the percentage between liquid and solid, said percentage being volume/weight percentage; the invention relates to the percentage between solids and liquids, the percentage being weight/volume percentage; the balance being weight/weight percent.
The invention was evaluated, unless otherwise indicated, using the following method:
1. acupoint selection: the acupoints are located according to the national standard of the people's republic of China (GB/T12345-2006) issued by the national technical supervision agency.
2. Facial paralysis movement function evaluation scale;
3. quality of life assessment scale for facial paralysis.
Compared with the prior art, the invention has the following beneficial effects:
1. the scientific combination pharmaceutical composition is used for treating the facial paralysis in the recovery period, and adopts simultaneous administration and/or sequential administration modes to treat the affected side, thereby being beneficial to alleviating facial paralysis edema, eliminating facial paralysis part inflammation, nourishing facial paralysis to damage nerves and repairing damaged myelin and axons. The dehydration medicine in the medicine composition is used for eliminating facial paralysis edema, and relieving or even eliminating facial nerve injury caused by the edema; the antiviral medicine is used for resisting viruses, eliminating the etiology causing facial paralysis and promoting nerve regeneration; the anti-inflammatory medicine is used for removing facial paralysis inflammatory factors, and is beneficial to removing facial paralysis inflammation and edema; the nerve repair medicine and/or the medicine for improving blood circulation is used for improving blood circulation disorder caused by facial paralysis edema, virus, inflammation and the like, repairing facial nerve injury caused by facial paralysis edema, virus, inflammation and the like, promoting vegetative nerve, promoting myelin and axon repair and the like; the Chinese medicinal preparation is used for dispelling wind and resolving phlegm, promoting blood circulation and removing obstruction in collaterals, and is beneficial to treatment, rehabilitation and prognosis of the facial paralysis in the convalescence.
2. The pharmaceutical composition of the invention combines radio frequency operation and rehabilitation operation, has the advantages of synergy, quick effect, high effective rate, high cure rate, basically no side effect, basically no recurrence rate and the like, and obviously improves the treatment prognosis and the quality of life of patients.
Detailed Description
The following detailed description of the invention is provided in connection with specific embodiments, but is not intended to limit the scope of the invention.
Example 1Preparation of neural repair cell protein extract with repair effect
1. Culture of primary mesenchymal Stem cells
The culture of primary mesenchymal stem cells comprises the following steps:
1) Cleaning umbilical cord, sterilizing, dissecting tissue, collecting the tissue of HUALONG gel layer, cutting into 3mm pieces 3 Is centrifuged, washed, tissue pieces are collected, placed in a culture flask, DMEM/F12 medium containing 10% fetal bovine serum FBS, 100ug/ml penicillin, 100ug/ml streptomycin is added, and then placed at 37 ℃ and 5% CO 2 Culturing under the condition to promote the adherence of the culture medium, observing the yellowing of the culture medium every 2-3 days, and half-changing the culture medium, and culturing for 10-12 days until the cells on the tissue block edge can climb out;
2) Slightly shaking to drop the tissue blocks, and respectively collecting the tissue blocks and lower cells, wherein the collected tissue blocks are subjected to wall-attached culture;
3) Washing the collected low-layer cells with PBS, adding a proper amount of 0.25% trypsin for digestion for 2-3 min, adding an equal volume of trypsin stopping solution for stopping digestion, lightly blowing a bottle bottom by a suction tube, centrifuging at 1500rpm for 5min, and collecting the cells.
2. Subculture of Primary mesenchymal Stem cells (culture of mesenchymal Stem cells)
Subculture of primary mesenchymal stem cells (culture of mesenchymal passage stem cells): the primary mesenchymal stem cells are prepared according to the initial density of 5.0x10 5 -5.0×10 6 Each ml was added to DMEM/F12 medium containing 10% FBS, 100U/ml penicillin and 100ug/ml streptomycin, and then placed at 37.0deg.C.+ -. 0.5 ℃ and 5%.+ -. 1% CO 2 Culturing under the condition for 10-15 days at intervals of 2-3 days, and half-changing the culture medium after observing the yellowing of the culture medium.
3. Preparation of Compounds 1-16 reference 1 (New limonophyllines A-C from the stem of Atalantia monophylla and cytotoxicity against cholangiocarcinoma and HepG2 cell lines, arch.Pharm.Res. (2018) 41:431-437).
The preparation method of the nerve repair cell protein extract with the nerve repair effect comprises the following steps:
(1) Mesenchymal passaged cells were passaged at a density of 8.0X10 6 Adding into culture medium containing DMEM/F1245%, RPMI1640 45%, bovine Serum Albumin (BSA) 0.5%, epidermal Growth Factor (EGF) 10ug/mL, fibroblast Growth Factor (FGF) 10ug/mL, insulin transferrin 10ug/mL, compound amino acid (18 AA) 0.05% and 5 μmol/L of compound 16, and placing at 37deg.C and 5% CO 2 After 4h incubation, cells were collected after centrifugation at 1200rpm for 5min and washing 3 times with PBS;
(2) The cells collected in step (1) were packed at a density of 1.0X10 7 Dispersing the cells/mL in physiological saline, and performing ultrasonic treatment at 2-8deg.C, 25kHz and 360W for 3s and 1s gap for 2min to obtain cell lysate;
(3) Centrifuging the cell lysate prepared in the step (2) at 7000rpm for 20min, and filtering the obtained centrifugate sequentially with 0.45um and 0.22um filter membrane to obtain cell protein extract;
(4) And (3) adding the required amount of mannitol into the cellular protein extract prepared in the step (3), stirring, uniformly mixing, and freeze-drying, wherein the obtained freeze-dried preparation contains 5% mannitol (m/m).
Test example 1The pharmaceutical composition is used for researching the treatment effect of the recovery phase facial paralysis
70 patients with facial paralysis in the recovery period are selected and divided into 1 group (10), 2 groups (40) and 3 groups (20). Patient inclusion criteria: meets the diagnosis standard of traditional Chinese medicine and Western medicine for facial neuritis, and the disease of patients exceeds 15 days to 6 months; age 20-70 years; the H-B is classified above the II level; informed consent was received for this trial; the compliance is good. Patient exclusion criteria: those with complete fracture or loss of facial nerves; a person with an infected or damaged puncture site; localized tumors or other focal lesions that involve facial nerves; a person with clotting dysfunction may increase the risk of bleeding or hematoma; cardiac pacemaker implanters may interfere with the proper functioning of the pacemaker; pregnant or lactating women, may affect the health of the fetus or infant; allergic reactions or other adverse reactions may be caused to the electrode needle or the patient allergic to the local anesthetic; mental disorders or those who cannot be treated with rehabilitation or corrective training.
Treatment regimen of group 1 (7 days per course, 4 courses of treatment): a single acupoint injection pharmaceutical composition is prepared from mouse nerve growth factor 30ug, mecobalamin 0.5mg, and vitamin B 1 200mg, selecting yang white acupoint, temple, sibai acupoint, yingxiang acupoint, juliao acupoint, dicang acupoint and Jiache acupoint of affected side portion for acupoint injection for 1 time per day.
The treatment regimen of group 2 (7 days per treatment course, 4 treatment courses) included pulsed rf surgery, post-operative oral administration and point injection administration, and intraoperative and post-operative rehabilitation exercises.
1. Pulsed radio frequency surgery: performing nerve activation repair regulation and control micro deviation correction operation (pulse width 20ms, resting period 480ms, maximum voltage value 100V, pulse frequency 2 HZ) on a lesion part based on a long-time temperature control high-voltage variable-frequency pulse radio frequency technology by adopting a radio frequency temperature control condenser (model R-2000B M1, purchased from Beiqi medical treatment), and pulse radio frequency for 1200 seconds under the conditions of 41-42 ℃, 90-140V and 2-5 Hz; in operation, 2 groups of open eyes are matched for holding, repeating the facial movements of grin teeth and repeated air blowing;
2. regimen of simultaneous and/or sequential administration of post-operative oral medications:
(1) Oral administration of aescin sodium 20 mg/time, 2 times/day, and administration for 1 month;
(2) 10 mg/time, 3 times/day of oral dibazol for 1 month;
(3) Oral mecobalamin 0.5 mg/time and adenosylcobalamin 0.5 mg/time, 3 times/day for 1 month;
(4) Orally administering ginkgo leaf 0.2 g/time, 3 times/day for 1 month;
(5) Oral administration of Ginseng radix reconstituted pill 3 g/time, 2 times/day, and administration for 1 month
3. Postoperative acupoint injection dosing scheme:
the single acupoint injection pharmaceutical composition consists of 30ug of mouse nerve growth factor, 0.5mg of mecobalamin, 0.5mg of adenosyl cobalamine, 5mg of dexamethasone and 1ml of lidocaine hydrochloride, and is prepared for clinical use, wherein yang white acupoint, temple, sibai acupoint, yingxiang acupoint, juliao acupoint, dicang acupoint and Jiache acupoint of the affected side part are selected for acupoint injection, and the acupoint injection is carried out 1 time per day.
4. Postoperative rehabilitation exercise training:
the first set of rehabilitation operation: first, a mirror; secondly, the eyes are slowly closed, after the eyes are closed for 5 seconds, the eyes are opened for 5 seconds, and meanwhile, the lifting action of the mouth angle connecting belt is controlled not to occur, and the correction of the connecting movement are carried out. Three groups were trained daily, each group being trained 30 times; the second set of rehabilitation operation: first, a mirror; secondly, eyes are opened, and silent nerve branches are kept as much as possible; and thirdly, pucker the mouth, teeth and cheeks. Three groups were trained daily, each group being trained 10 times.
The treatment regimen of group 3 (7 days per treatment course, 4 treatment courses) included pulsed rf surgery, post-operative oral administration and point injection administration, and intraoperative and post-operative rehabilitation exercises.
1. Pulsed radio frequency surgery: performing nerve activation repair regulation and control micro deviation correction operation (pulse width 20ms, resting period 480ms, maximum voltage value 100V, pulse frequency 2 HZ) on a lesion part based on a long-time temperature control high-voltage variable-frequency pulse radio frequency technology by adopting a radio frequency temperature control condenser (model R-2000B M1, purchased from Beiqi medical treatment), and pulse radio frequency for 1200 seconds under the conditions of 41-42 ℃, 90-140V and 2-5 Hz; in operation, 2 groups of open eyes are matched for holding, repeating the facial movements of grin teeth and repeated air blowing;
2. regimen of simultaneous and/or sequential administration of post-operative oral medications:
(1) Oral administration of aescin sodium 20 mg/time, 2 times/day, and administration for 1 month;
(2) 10 mg/time, 3 times/day of oral dibazol for 1 month;
(3) Oral mecobalamin 0.5 mg/time and adenosylcobalamin 0.5 mg/time, 3 times/day for 1 month;
(4) Orally administering ginkgo leaf 0.2 g/time, 3 times/day for 1 month;
(5) Orally taking 3 g/time, 2 times/day of ginseng reconstruction pill, and taking 1 month;
3. postoperative acupoint injection dosing scheme:
(1) The single acupoint injection pharmaceutical composition consists of 30ug of mouse nerve growth factor, 0.5mg of mecobalamin, 0.5mg of adenosyl cobalamine, 5mg of dexamethasone and 1ml of lidocaine hydrochloride, is prepared for clinical use, and is prepared by selecting yang white acupoint, temple, sibai acupoint, yingxiang acupoint, juliao acupoint, dicang acupoint and Jiache acupoint of the affected side part for acupoint injection for 1 time every day;
(2) The nerve repairing cell protein extract freeze-dried preparation 130ug prepared in example 1 is injected into a single acupoint, dissolved in 2ml physiological saline, and selected from yang white acupoint, temple acupoint, four white acupoint, yingxiang acupoint, juliao acupoint, dicang acupoint and Jiache acupoint, contralateral temple acupoint and Dicang acupoint, and double-hand Hegu acupoint for acupoint injection for 1 time every day.
4. Rehabilitation training:
the first set of rehabilitation operation: first, a mirror; secondly, the eyes are slowly closed, after the eyes are closed for 5 seconds, the eyes are opened for 5 seconds, and meanwhile, the lifting action of the mouth angle connecting belt is controlled not to occur, and the correction of the connecting movement are carried out. Three groups were trained daily, each group being trained 30 times; the second set of rehabilitation operation: first, a mirror; secondly, the eyes are opened to keep silent nerve branches as much as possible; and thirdly, pucker the mouth, teeth and cheeks. Three groups were trained daily, each group being trained 10 times.
Efficacy assessment: satisfaction questionnaires and clinical scores House-Brakmann facial nerve function grading efficacy evaluation forms.
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Group 1: for 3 days of treatment, about 50% of the patient's facial muscles are improved; the effective rate of the medicine for 28 days is 70%.
Group 2: for 3 days of treatment, about 60% of the patient's facial muscles improve; the effective rate of the medicine is 80% after 7 days of treatment. The cure rate for 28 days of treatment was about 90%. The patient follows the doctor's advice, pay attention to reduce or even avoid the influence of factors such as staying up all night, tired, external infection, catching cold, etc., basically no recurrence, the quality of life is showing and is improving.
Group 3: about 80% of the facial muscles of the patient are improved after 3 days of treatment, and the effective rate of the treatment is 90% after 7 days. The cure rate of 28 days of treatment was about 95%. The patient follows the doctor's advice, pay attention to reduce or even avoid the influence of factors such as staying up all night, tired, external infection, catching cold, etc., basically no recurrence, the quality of life is showing and is improving.
The above description of the embodiments of the present invention is not intended to limit the present invention, and those skilled in the art can make various changes or modifications according to the present invention without departing from the spirit of the present invention, and shall fall within the scope of the claims of the present invention.

Claims (10)

1. The application of the pharmaceutical composition in preparing medicines for preventing and treating the convalescence facial paralysis comprises any one or combination of dehydration medicines, antiviral medicines, anti-inflammatory medicines, nerve repair medicines and/or medicines for improving blood circulation and traditional Chinese medicine preparations.
2. The use according to claim 1, wherein the pharmaceutical composition for preventing and treating convalescence facial paralysis comprises any one of mannitol, sodium aescinate, acyclovir, valacyclovir, dibazol, dexamethasone, prednisone, citicoline sodium, ginkgo leaf, murine nerve growth factor, mecobalamin, adenosylcobalamin or a combination thereof.
3. The use according to any one of claims 1-2, wherein the pharmaceutical composition for preventing and treating convalescence facial paralysis consists of a pharmaceutical composition for single oral administration and a pharmaceutical composition for single acupoint injection.
4. The use according to any one of claims 1-3, wherein the pharmaceutical composition for single oral administration comprises aescin sodium 20-40mg, dibazol 10-30mg, mecobalamin 0.5-1.5mg, adenosylcobalamin 0.5-1.5mg, ginkgo leaf 0.2-0.6g and ginseng re-pill 3-6g.
5. The use according to any one of claims 1 to 4, wherein the oral pharmaceutical composition is administered according to the following regimen:
(1) Oral administration of aescin sodium 20 mg/time, 2 times/day, and administration for 1 month;
(2) 10 mg/time, 3 times/day of oral dibazol for 1 month;
(3) Oral mecobalamin 0.5 mg/time and adenosylcobalamin 0.5 mg/time, 3 times/day for 1 month;
(4) Orally administering ginkgo leaf 0.2 g/time, 3 times/day for 1 month;
(5) The oral administration of the ginseng regeneration pill is 3 g/time, 2 times/day, and 1 month.
6. The use according to any one of claims 1 to 5, wherein the pharmaceutical composition for preventing and treating convalescence facial paralysis is optionally used in combination with any one of radio frequency surgery, rehabilitation training or a combination thereof.
7. The use according to any one of claims 1-6, wherein the radiofrequency operation is based on long-term temperature controlled high voltage variable frequency pulses at 41-42 ℃, 90V-140V, 2Hz-6Hz for 800-1400 seconds.
8. A pharmaceutical composition for preventing and treating convalescence facial paralysis contains any one or combination of dehydration drugs, antiviral drugs, anti-inflammatory drugs, nerve repair drugs and/or drugs for improving blood circulation and Chinese medicinal preparations.
9. The pharmaceutical composition of claim 8, optionally in combination with any one of radio frequency surgery, rehabilitation training procedure or a combination thereof, for the prevention and treatment of convalescence facial paralysis.
10. A therapeutic regimen for the prevention and treatment of convalescent facial paralysis comprising a pharmaceutical composition for the prevention and treatment of convalescent facial paralysis according to any one of claims 8-9, optionally in combination with any one of radio frequency surgery, rehabilitation training procedures, or a combination thereof.
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