CN117652254B - Detoxification preservation method of sweet potato seeds - Google Patents
Detoxification preservation method of sweet potato seeds Download PDFInfo
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- CN117652254B CN117652254B CN202311645794.2A CN202311645794A CN117652254B CN 117652254 B CN117652254 B CN 117652254B CN 202311645794 A CN202311645794 A CN 202311645794A CN 117652254 B CN117652254 B CN 117652254B
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- 244000017020 Ipomoea batatas Species 0.000 title claims abstract description 86
- 235000002678 Ipomoea batatas Nutrition 0.000 title claims abstract description 85
- 238000004321 preservation Methods 0.000 title claims abstract description 46
- 238000000034 method Methods 0.000 title claims abstract description 39
- 238000001784 detoxification Methods 0.000 title claims abstract description 33
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 123
- 241000234314 Zingiber Species 0.000 claims abstract description 101
- 235000006886 Zingiber officinale Nutrition 0.000 claims abstract description 101
- 235000008397 ginger Nutrition 0.000 claims abstract description 101
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims abstract description 84
- 230000001954 sterilising effect Effects 0.000 claims abstract description 72
- 239000007788 liquid Substances 0.000 claims abstract description 49
- 238000002156 mixing Methods 0.000 claims abstract description 17
- 238000009835 boiling Methods 0.000 claims abstract description 16
- 238000001914 filtration Methods 0.000 claims abstract description 16
- 238000005507 spraying Methods 0.000 claims abstract description 15
- 238000004140 cleaning Methods 0.000 claims abstract description 9
- 238000003860 storage Methods 0.000 claims abstract description 9
- 230000001105 regulatory effect Effects 0.000 claims abstract description 7
- 238000001035 drying Methods 0.000 claims abstract description 6
- 239000000706 filtrate Substances 0.000 claims abstract description 6
- 238000012360 testing method Methods 0.000 claims description 16
- 239000000645 desinfectant Substances 0.000 claims description 15
- 238000009395 breeding Methods 0.000 claims description 9
- 240000005384 Rhizopus oryzae Species 0.000 claims description 6
- 235000013752 Rhizopus oryzae Nutrition 0.000 claims description 6
- 239000001963 growth medium Substances 0.000 claims description 6
- 241000235546 Rhizopus stolonifer Species 0.000 claims description 5
- 241001360526 Escherichia coli ATCC 25922 Species 0.000 claims description 3
- 241000191967 Staphylococcus aureus Species 0.000 claims description 3
- 238000000338 in vitro Methods 0.000 claims description 2
- 230000000844 anti-bacterial effect Effects 0.000 abstract description 5
- 238000004134 energy conservation Methods 0.000 abstract 1
- 230000007613 environmental effect Effects 0.000 abstract 1
- 238000004659 sterilization and disinfection Methods 0.000 description 18
- NLDDIKRKFXEWBK-AWEZNQCLSA-N gingerol Chemical compound CCCCC[C@H](O)CC(=O)CCC1=CC=C(O)C(OC)=C1 NLDDIKRKFXEWBK-AWEZNQCLSA-N 0.000 description 15
- JZLXEKNVCWMYHI-UHFFFAOYSA-N gingerol Natural products CCCCC(O)CC(=O)CCC1=CC=C(O)C(OC)=C1 JZLXEKNVCWMYHI-UHFFFAOYSA-N 0.000 description 14
- 235000002780 gingerol Nutrition 0.000 description 14
- 241000700605 Viruses Species 0.000 description 8
- 241000894006 Bacteria Species 0.000 description 7
- 201000010099 disease Diseases 0.000 description 7
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 7
- 244000052616 bacterial pathogen Species 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- 230000005764 inhibitory process Effects 0.000 description 6
- 230000001717 pathogenic effect Effects 0.000 description 6
- 230000001488 breeding effect Effects 0.000 description 5
- 230000008859 change Effects 0.000 description 5
- 238000001816 cooling Methods 0.000 description 5
- 241000196324 Embryophyta Species 0.000 description 4
- 244000061456 Solanum tuberosum Species 0.000 description 4
- 235000002595 Solanum tuberosum Nutrition 0.000 description 4
- 239000003242 anti bacterial agent Substances 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 239000003112 inhibitor Substances 0.000 description 4
- 230000004048 modification Effects 0.000 description 4
- 238000012986 modification Methods 0.000 description 4
- OQWKEEOHDMUXEO-UHFFFAOYSA-N (6)-shogaol Natural products CCCCCC=CC(=O)CCC1=CC=C(O)C(OC)=C1 OQWKEEOHDMUXEO-UHFFFAOYSA-N 0.000 description 3
- OQWKEEOHDMUXEO-BQYQJAHWSA-N [6]-Shogaol Chemical compound CCCCC\C=C\C(=O)CCC1=CC=C(O)C(OC)=C1 OQWKEEOHDMUXEO-BQYQJAHWSA-N 0.000 description 3
- 230000003385 bacteriostatic effect Effects 0.000 description 3
- 239000013068 control sample Substances 0.000 description 3
- 229910052739 hydrogen Inorganic materials 0.000 description 3
- 239000001257 hydrogen Substances 0.000 description 3
- -1 hydrogen ions Chemical class 0.000 description 3
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 3
- 239000003755 preservative agent Substances 0.000 description 3
- 230000002335 preservative effect Effects 0.000 description 3
- 239000000523 sample Substances 0.000 description 3
- 238000002791 soaking Methods 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- 208000035143 Bacterial infection Diseases 0.000 description 2
- 230000004075 alteration Effects 0.000 description 2
- 208000022362 bacterial infectious disease Diseases 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 244000052769 pathogen Species 0.000 description 2
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 description 2
- 239000013641 positive control Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000008223 sterile water Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
- 206010014418 Electrolyte imbalance Diseases 0.000 description 1
- 241000235527 Rhizopus Species 0.000 description 1
- PQLVXDKIJBQVDF-UHFFFAOYSA-N acetic acid;hydrate Chemical compound O.CC(O)=O PQLVXDKIJBQVDF-UHFFFAOYSA-N 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 230000002421 anti-septic effect Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 238000013400 design of experiment Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 125000004435 hydrogen atom Chemical group [H]* 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/90—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in food processing or handling, e.g. food conservation
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Soil Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention belongs to the technical field of sweet potato preservation, and particularly relates to a sweet potato seed detoxification preservation method. Comprising the following steps: cleaning the harvested sweet potato seeds, and draining water; spraying a sterilizing liquid on the surface of sweet potato seeds, wherein the sterilizing liquid comprises the following formula: 10-30g of high-concentration ginger water, 1kg of water and acetic acid for regulating the pH value to 3-5; the high-concentration ginger water is obtained by mixing ginger and water according to the mass ratio of 1:6-8, boiling, and filtering the obtained filtrate; drying sweet potato seeds until the water content is 60-70%; is placed in a storage room for preservation. The sweet potato seed detoxification preservation method provided by the invention only needs the sterilizing liquid with one formula, and the sterilizing liquid has the advantages of simple components, energy conservation, environmental protection and antibacterial effect.
Description
Technical Field
The invention belongs to the technical field of sweet potato preservation, and particularly relates to a sweet potato seed detoxification preservation method.
Background
The sweet potato species may have pathogenic components such as bacteria, mycoviruses and the like, so that disease seedlings or dead seedlings appear in the breeding process. Therefore, those skilled in the art often select stem tips with less pathogenic components as breeding materials, and then combine the steps of detoxification treatment, culture, virus detection and the like to obtain detoxified seedlings which are used for breeding. In the cultivation process of sweet potato resources, any one of the detoxification method, the propagation method and the preservation method is improper, and can cause the sweet potato to generate pathogenic components. The preservation period is longer, is the period in which pathogenic components are most likely to be generated, reasonably preserves sweet potato seeds, reduces pollution of the pathogenic components as much as possible, and can reduce disinfection work of the later detoxification and breeding steps. Therefore, protection against viral contamination during storage of sweet potatoes is a common resort to by those skilled in the art.
Chinese patent CN107114467A discloses a sweet potato storage method, which adopts cleaning liquid, antibacterial agent, bud inhibitor and the like with special formulas, the sweet potato is firstly cleaned by the cleaning liquid with special formulas after being picked, then is dried, is sterilized by the antibacterial liquid, is then treated by the bud inhibitor, and is finally buried in mixed river sand, and the method can reduce mildew and rot of the sweet potato. Chinese patent CN108738468a also developed preservative and antiseptic solutions for controlling viruses and bacteria. There are other methods of storing sweet potatoes such as CN106720240a to prevent decay. The prior art shows the achievement of researchers in solving the pollution of sweet potato pathogenic components, but the components of cleaning liquid, antibacterial agent, bud inhibitor, preservative solution and sterilizing solution as described in CN107114467A, CN108738468A are complex, and especially the antibacterial agent mentioned in the above patent contains various extract components, and can be prepared by specific extraction means. Therefore, there is a need to develop a bacteriostatic method that is simple in ingredients but still has an antibacterial effect.
Disclosure of Invention
In order to solve the technical problems of complex components of cleaning liquid, antibacterial agent, bud inhibitor, preservative solution or sterilizing solution in the prior art, the invention provides a sweet potato seed detoxification preservation method, which only needs sterilizing solution with one formula, has simple components, has antibacterial effect, and is energy-saving and environment-friendly.
The invention aims to provide a sweet potato seed detoxification preservation method, which comprises the following steps:
Washing the harvested sweet potato seeds with water, and draining off water;
spraying a sterilizing liquid on the surface of the sweet potato seeds; the formula of the disinfectant is as follows: 10-30g of high-concentration ginger water, 1kg of water and acetic acid for regulating the pH value to 3-5;
The high-concentration ginger water is obtained by mixing ginger and water according to a mass ratio of 1:6, boiling for 20min, and filtering off ginger to obtain filtrate;
drying sweet potato seeds until the water content is 60-70%;
Is placed in a storage room for preservation.
The formula of the disinfectant disclosed by the invention takes ginger water as a main component for sterilization, the ginger water contains sterilization components such as gingerol, gingerol and shogaol, and the gingers contain gingerol, gingerol and gingerol which are commonly called as gingerol and can kill germs.
The water is domestic drinking water conforming to national standards, such as tap water, mineral water and the like.
In one embodiment of the invention, the sweet potato varieties are Ji potato No. 18, guangdong potato No. 135, ning purple No. 4 and Beijing potato No. 6, which are sweet potato varieties suitable for local planting and breeding in Hubei province, which are obtained by local materials and are tested in situ, and according to the tests, the disinfectant provided by the test has the detoxification effect on the varieties.
Preferably, the sweet potato seed detoxification preservation method and the killing solution are prepared at present.
The mode of the present invention is adopted because, regarding the principle of the disinfectant of the present invention for resisting bacteria and viruses, we have the following understanding: a gingerol has a structural formula I shown in the specification, wherein CAS number 23513-14-6 contains benzene ring, hydroxyl and other groups, while a solution with low pH contains a large amount of hydrogen ions, and when the pH is about 4.5, the hydrogen groups/hydrogen ions react with the hydroxyl to cause the group arrangement and polarity change of substances, and the polarity change causes the disturbance of bioelectric signals in bacteria and viruses, so that the effects of sterilization and virus killing are achieved. Because bioelectric signals are important factors for transmitting information and regulating physiological functions of organisms, metabolic activities of the organisms can be influenced when the bioelectric signals are disturbed, such as severe reactions such as diarrhea, dehydration and the like can occur after electrolyte disturbance of a human body. The invention utilizes the principle to achieve the effects of sterilization and virus killing, and experimental results also show that the sterilization effect of the existing prepared sterilization liquid is more obvious.
Preferably, in the sweet potato seed detoxification and preservation method, the sterilizing liquid is prepared for 1 h.
Preferably, in the detoxification preservation method of sweet potato seeds, acetic acid is used for adjusting the pH value of water to 3-5, the water is sprayed on the surfaces of sweet potatoes, and then high-concentration ginger water with a formula amount is sprayed.
Preferably, the sterilization and preservation method for sweet potato seeds has the spraying amount of 10-20ml/kg.
Preferably, according to the sweet potato seed detoxification preservation method, the spraying amount of the sterilizing liquid is 15ml/kg.
Preferably, in the sweet potato seed detoxification and preservation method, the formula of the disinfection solution is as follows: 30g of high-concentration ginger water, 1kg of water and acetic acid to adjust the pH to 4.5.
Preferably, in the sweet potato seed detoxification preservation method, the high-concentration ginger water is obtained by mixing ginger and water according to a mass ratio of 1:6, boiling for 20min, and filtering ginger to obtain filtrate.
Preferably, according to the sweet potato seed detoxification preservation method, the ginger is cleaned and then high-concentration ginger water is prepared, so that germs carried by the ginger are reduced.
Preferably, in the detoxification preservation method of the sweet potato seeds, in order to avoid the pollution of germs in ginger to sweet potatoes as much as possible, high-concentration ginger water is placed at 121 ℃ for sterilization for 30min.
A sweet potato detoxification breeding method, comprising: taking the stem tip meristem of the sweet potato under the aseptic condition, sterilizing by using the sterilizing liquid, and inoculating the stem tip of the peeled leaf primordium on a culture medium for in vitro culture until the stem tip is cultured into test tube seedlings.
Compared with the prior art, the invention has the following beneficial effects:
The formula of the sterilizing liquid takes ginger water AS a main sterilization component, the ginger water contains sterilizing components such AS gingerol, gingerol and shogaol, and the ginger contains gingerol, gingerol and shogaol, which are collectively called AS gingerol, and the gingerol can kill germs, and the researches show that the ginger water can also kill rhizopus stolonifer standard strains, rhizopus arrhizus standard strains, rhizopus oryzae AS3.3461, rhizopus nigrum AS3.4108, staphylococcus aureus and escherichia coli ATCC 25922. Since most pathogens have a pH suitable for growth and reproduction, our low pH environment provided by acetic acid can kill most pathogens.
The structural formula of gingerol contains groups such as benzene ring and hydroxyl, and the solution with low pH contains a large amount of hydrogen ions, which can react to cause the arrangement of the groups of substances and the change of polarity, and the polarity change causes the disturbance of bioelectric signals in bacteria and viruses, thereby achieving the effect of sterilization and virus killing. The experimental result of the invention also shows that the sterilizing effect of the sterilizing liquid prepared at present is more obvious.
Drawings
Fig. 1 is a technical route diagram of the sweet potato seed detoxification preservation method of the invention.
Detailed Description
In order that those skilled in the art will better understand the technical scheme of the present invention, the present invention will be further described with reference to specific embodiments and drawings.
In the description of the present invention, unless otherwise specified, all reagents are commercially available and methods are conventional in the art. The sweet potato seeds used in the following examples are Ji potato No. 18 sweet potato seeds. The ginger is fresh ginger, and when the high-concentration ginger water is prepared, the ginger is weighed by washing and draining.
Example 1
A formula of a sterilizing liquid suitable for sweet potato detoxification preservation comprises the following components: 10g of high-concentration ginger water, 1kg of water and acetic acid to adjust the pH to 4.5. The high-concentration ginger water is obtained by mixing ginger and water according to a mass ratio of 1:6, boiling for 20min, and filtering off ginger.
Example 2
A formula of a sterilizing liquid suitable for sweet potato detoxification preservation comprises the following components: high concentration ginger water 20g, water 1kg, acetic acid to adjust pH to 4.5. The high-concentration ginger water is obtained by mixing ginger and water according to a mass ratio of 1:6, boiling for 20min, and filtering off ginger.
Example 3
A formula of a sterilizing liquid suitable for sweet potato detoxification preservation comprises the following components: 30g of high-concentration ginger water, 1kg of water and acetic acid to adjust the pH to 4.5. The high-concentration ginger water is obtained by mixing ginger and water according to a mass ratio of 1:6, boiling for 20min, and filtering off ginger.
Example 4
A formula of a sterilizing liquid suitable for sweet potato detoxification preservation comprises the following components: 10g of high-concentration ginger water, 1kg of water and acetic acid to adjust the pH to 3. The high-concentration ginger water is obtained by mixing ginger and water according to a mass ratio of 1:6, boiling for 20min, and filtering off ginger.
Example 5
A formula of a sterilizing liquid suitable for sweet potato detoxification preservation comprises the following components: 10g of high-concentration ginger water, 1kg of water and acetic acid to adjust the pH to 3.5. The high-concentration ginger water is obtained by mixing ginger and water according to a mass ratio of 1:6, boiling for 20min, and filtering off ginger.
Example 6
A formula of a sterilizing liquid suitable for sweet potato detoxification preservation comprises the following components: 10g of high-concentration ginger water, 1kg of water and acetic acid to adjust the pH to 4. The high-concentration ginger water is obtained by mixing ginger and water according to a mass ratio of 1:6, boiling for 20min, and filtering off ginger.
Example 7
A formula of a sterilizing liquid suitable for sweet potato detoxification preservation comprises the following components: 10g of high-concentration ginger water, 1kg of water and acetic acid to adjust the pH to 5. The high-concentration ginger water is obtained by mixing ginger and water according to a mass ratio of 1:6, boiling for 20min, and filtering off ginger.
1. The bacteriostasis experiments for examples 1-7 were as follows:
(1) Principle of testing
The inhibition zone test is to utilize the sterilizing liquid to be continuously dissolved and spread by agar to form different concentration gradients, and the antibacterial performance of the sterilizing liquid can be evaluated by recording the size of the inhibition zone.
(2) Design of experiment
Test sample: sterile drying filter paper with diameter of 5mm, dropwise adding 20 μl of sterilizing liquid into each piece, and drying. Note that 5 parallel test samples were made for each example of the disinfectant bacteria.
Blank control sample: the control sample for the inhibition zone test is sterile dry filter paper with the diameter of 5mm, and 20 mu L of sterile water is added dropwise to each piece of filter paper for drying.
Positive control sample 1: sterile dried filter paper with a diameter of 5mm was added dropwise 20. Mu.L of A positive solution per piece, and dried. The A positive liquid formula is as follows: the pH of the high-concentration ginger water is regulated to 4.5 by 10g and acetic acid. Only the bacteriostatic effect of the high-concentration ginger water is studied. The high-concentration ginger water is obtained by mixing ginger and water according to a mass ratio of 1:6, boiling for 20min, and filtering off ginger.
Positive control sample 2: sterile dried filter paper with a diameter of 5mm was added dropwise 20. Mu.L of the B positive solution per piece, and dried. The formula of the B positive liquid is as follows: 1kg of water and acetic acid to adjust the pH to 4.5. Only the bacteriostatic effect of the acetic acid-water mixture was studied.
(2) All experimental equipment, experimental reagents and samples are sterilized first and then are put to room temperature for standby.
(3) Test strain
Standard strain of rhizopus stolonifer, shanghai, amersham biosciences.
Rhizopus arrhizus standard strain, shanghai, amersham biosciences.
Rhizopus oryzae AS3.3461, shanghai, calif., a company of Biotechnology, inc.
Rhizopus nigricans AS3.4108, shanghai, inc. of Biotech.
Staphylococcus aureus, seashell, biotechnology limited.
Escherichia coli ATCC 25922, north Biotechnology Co., ltd.
(4) Data processing
The diameter of the inhibition zone was measured with a vernier caliper and recorded. The control sample should have no zone of inhibition.
The value of the inhibition zone is taken as the average value of 5 samples, and 2 valid figures are reserved.
(5) Experimental results
Table 1 results of bacteriostasis experiments
Example 8
A method for detoxication preservation of sweet potato seeds comprises the following steps:
And (5) cleaning the harvested sweet potato seeds with water, and draining off water.
Spraying sterilizing liquid on the surface of sweet potato seeds, and cooling to room temperature, wherein the spraying amount of the sterilizing liquid is 15ml/kg. The formula of the disinfectant is as follows: 30g of high-concentration ginger water, 1kg of water and acetic acid to adjust the pH to 4.5. The sterilization conditions of the sterilizing liquid are as follows: sterilizing at 121deg.C for 30min. The disinfectant is used in the configuration 12 h.
The high-concentration ginger water is obtained by mixing ginger and water according to a mass ratio of 1:6, boiling for 20min, and filtering off ginger.
The sweet potato seeds were then dried to a moisture content of 60%.
The plant is placed in a storage room for preservation, and no disease occurs in 4 months.
Example 9
A method for detoxication preservation of sweet potato seeds comprises the following steps:
And (5) cleaning the harvested sweet potato seeds with water, and draining off water.
Spraying a sterilizing liquid on the surface of the sweet potato seeds, wherein the spraying amount of the sterilizing liquid is 15ml/kg. The formula of the disinfectant is as follows: 30g of high-concentration ginger water, 1kg of water and acetic acid to adjust the pH to 4.5. The sterilization conditions of the sterilizing liquid are as follows: sterilizing at 121deg.C for 30min. Sterilizing the sterilizing liquid, cooling to room temperature, and reusing. The disinfectant is used in the configuration 12 h.
The high-concentration ginger water is obtained by mixing ginger and water according to a mass ratio of 1:8, boiling for 40min, and filtering the ginger to obtain a filtrate.
The sweet potato seeds were then dried to a moisture content of 65%.
The plant is placed in a storage room for preservation, and no disease occurs in 4 months.
Example 10
A method for detoxication preservation of sweet potato seeds comprises the following steps:
Washing the harvested sweet potato seeds with water, and draining off water;
Spraying sterilizing liquid on the surface of sweet potato seeds, and cooling to room temperature, wherein the spraying amount of the sterilizing liquid is 15ml/kg. The formula of the disinfectant is as follows: 30g of high-concentration ginger water, 1kg of water and acetic acid to adjust the pH to 4.5; the sterilization conditions of the sterilizing liquid are as follows: sterilizing at 121deg.C for 30min. The disinfectant is used in the configuration 12 h.
The high-concentration ginger water is obtained by mixing ginger and water according to a mass ratio of 1:7, boiling for 30min, and filtering the ginger to obtain filtrate.
The sweet potato seeds were then dried to a moisture content of 70%.
The plant is placed in a storage room for preservation, no disease occurs in 4 months, and the disease occurrence rate in 5 months is 15%.
Example 11
A method for detoxication preservation of sweet potato seeds comprises the following steps:
And (5) cleaning the harvested sweet potato seeds with water, and draining off water.
Spraying a sterilizing liquid on the surface of the sweet potato seeds, wherein the spraying amount of the sterilizing liquid is 15ml/kg.
The formula of the disinfectant is as follows: high concentration ginger water 20g, water 1kg, acetic acid to adjust pH to 4.5. Wherein, the high-concentration ginger water and water are separated for use. Wherein, the high-concentration ginger water is sterilized at 121 ℃ for 30min and then cooled to room temperature for standby. Wherein, the pH of the water is adjusted to 4.5 by acetic acid, sterilized and cooled to room temperature for standby. Wherein, the formula amount of water (the pH value is adjusted to 4.5 by acetic acid) is sprayed on the surface of the sweet potato, and then the formula amount of high-concentration ginger water is immediately sprayed.
Wherein the high-concentration ginger water is obtained by mixing ginger and water according to a mass ratio of 1:7, boiling for 30min, and filtering off ginger.
The sweet potato seeds were then dried to a moisture content of 70%.
The plant is placed in a storage room for preservation, and no disease occurs for 5 months.
The disease occurrence result of the sweet potato seed detoxification preservation method can show that the sterilization effect is optimal when the sterilizing liquid is prepared and used.
Example 12
A sweet potato detoxification breeding method, comprising: taking the sweet potato stem tip meristem under aseptic conditions by utilizing the principle that the sweet potato stem tip part tissue hardly carries germs, soaking and sterilizing for 90s by using the sterilizing liquid of the example 3 (the sterilizing effect of the example 3 is best according to a bacteriostasis experiment), wherein the sterilizing liquid is prepared by mixing all raw materials, sterilizing and cooling to room temperature for 12 h. The stem tip with a leaf primordium with the stripping length of 0.2-0.3mm is inoculated on an MS culture medium for isolated culture, wherein 6-BA is added into the MS culture medium, the concentration is 1mg/L, and the test tube plantlet is cultured under the condition of 26 ℃ and the bacterial infection rate of the test tube plantlet is 8%.
Example 13
A sweet potato detoxification breeding method, comprising: taking the sweet potato stem tip meristem under aseptic conditions by utilizing the principle that the sweet potato stem tip part tissue hardly carries germs, soaking and sterilizing with the sterilizing liquid of the example 3 (the sterilizing effect of the example 3 is best according to a bacteriostasis experiment), wherein high-concentration ginger water is sterilized at 121 ℃ for 30min and cooled to room temperature for later use; regulating the pH of water to 4.5 with acetic acid, sterilizing, and cooling to room temperature for use; during soaking and disinfection, the fresh ginger is soaked in sterilized acetic acid regulated water for 45s and then soaked in sterilized high-concentration fresh ginger water for 45s. The stem tip with a leaf primordium with the stripping length of 0.2-0.3mm is inoculated on an MS culture medium for isolated culture, wherein 6-BA is added into the MS culture medium, the concentration is 1mg/L, and the test tube plantlet is cultured under the condition of 26 ℃ and the bacterial infection rate of the test tube plantlet is 4%.
Sterile water is used as a replacement disinfection solution, and as a control group of the embodiment 12 and the embodiment 13, the test tube plantlet of the test control group has a bacterial contamination rate of 31%.
In the present invention, when the high-concentration ginger water is separated from acetic acid, the pH of the high-concentration ginger water should be adjusted to 5 by sterilized acetic acid and then used for 5 minutes, so that the pH of the high-concentration ginger water is still close to 4.5 after the high-concentration ginger water is mixed with the water with the pH of 4.5. For example, in examples 11 and 13, the pH of the high concentration ginger water was adjusted to 5 with sterilized acetic acid and then used within 5 minutes.
In addition, the pH of the high-concentration ginger water is adjusted to 5 instead of 4.5, because the pH of the ginger water is 4.5, the inside of the ginger water is changed in groups and polarities, if the ginger water is not used in time, bioelectric signals of bacteria are difficult to influence, and the pH of the ginger water is close to 4.5, but the polarity change in the ginger water is not obvious at the moment.
It should be noted that, when numerical ranges are referred to in the present invention, it should be understood that two endpoints of each numerical range and any numerical value between the two endpoints are optional, and because the adopted step method is the same as the embodiment, in order to prevent redundancy, the present invention describes a preferred embodiment. While preferred embodiments of the present invention have been described, additional variations and modifications in those embodiments may occur to those skilled in the art once they learn of the basic inventive concepts. It is therefore intended that the following claims be interpreted as including the preferred embodiments and all such alterations and modifications as fall within the scope of the invention.
It will be apparent to those skilled in the art that various modifications and variations can be made to the present invention without departing from the spirit or scope of the invention. Thus, it is intended that the present invention also include such modifications and alterations insofar as they come within the scope of the appended claims or the equivalents thereof.
Claims (7)
1. The sweet potato seed detoxification preservation method is characterized by comprising the following steps:
cleaning the harvested sweet potato seeds, and draining water;
Spraying a sterilizing liquid on the surface of sweet potato seeds, wherein the sterilizing liquid comprises the following formula: 10-30g of high-concentration ginger water, 1kg of water and acetic acid for regulating the pH value to 3-5;
Drying sweet potato seeds until the water content is 60-70%;
Placing in a storage room for preservation;
The high-concentration ginger water is obtained by mixing ginger and water according to a mass ratio of 1:6, boiling for 20min, and filtering off ginger to obtain filtrate;
the disinfectant is used within 1 h;
the disinfectant is used for killing rhizopus stolonifer, rhizopus arrhizus, rhizopus oryzae AS3.3461, rhizopus nigricans AS3.4108, staphylococcus aureus and escherichia coli ATCC 25922.
2. The method for detoxication preservation of sweet potato seeds according to claim 1, wherein acetic acid is used to adjust the pH of water to 3-5, sprayed onto the surface of sweet potato, and then high-concentration ginger water is sprayed in a formula amount.
3. The method for detoxified preservation of sweet potato seeds according to claim 1, wherein the spraying amount of the sterilizing liquid is 10-20ml/kg.
4. The method for detoxified preservation of sweet potato seeds according to claim 3, wherein the spraying amount of the sterilizing liquid is 15ml/kg.
5. The method for detoxication preservation of sweet potato seeds according to claim 1, wherein the disinfectant is prepared from the following components: 30g of high-concentration ginger water, 1kg of water and acetic acid to adjust the pH to 4.5.
6. The method for detoxified preservation of sweet potato seeds of claim 1, wherein high concentration ginger water is sterilized at 121 ℃ for 30min.
7. The sweet potato detoxification breeding method is characterized by comprising the following steps: taking the stem tip meristem of sweet potato under aseptic condition, sterilizing with the sterilizing liquid of claim 1, and inoculating the stem tip with leaf primordium on a culture medium for in vitro culture until the test tube plantlet is cultured.
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| CN108738468A (en) * | 2018-05-24 | 2018-11-06 | 吴莉 | A method of storage sweet potato potato seed |
| CN108935108A (en) * | 2018-09-29 | 2018-12-07 | 河南云帮农业科技有限公司 | A kind of sweet potato tissue-cultured seedling detoxification tissue culture method |
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| CN106134723A (en) * | 2016-06-30 | 2016-11-23 | 普洱绿海生物开发有限公司 | A kind of detoxification implantation methods of gold zone Radix Ipomoeae |
| CN107114467A (en) * | 2017-05-13 | 2017-09-01 | 蚌埠市乔峰农业蔬菜专业合作社 | A kind of storage practice of sweet potato |
| CN107568001A (en) * | 2017-09-19 | 2018-01-12 | 紫云自治县文烁植保农民专业合作社 | One kind makees kind of a method for culturing seedlings using red core sweet potato vine |
| CN109197590A (en) * | 2018-10-24 | 2019-01-15 | 河南云帮农业科技有限公司 | A kind of sweet potato detoxication and tissue culture strong sprout method |
| KR102176033B1 (en) * | 2020-05-28 | 2020-11-06 | 주식회사 하나모아 | Ginger water composition having ginger extract and method for manufacturing the same |
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| CN108738468A (en) * | 2018-05-24 | 2018-11-06 | 吴莉 | A method of storage sweet potato potato seed |
| CN108935108A (en) * | 2018-09-29 | 2018-12-07 | 河南云帮农业科技有限公司 | A kind of sweet potato tissue-cultured seedling detoxification tissue culture method |
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