CN117629987A - Sex identification method for chick embryo eggs - Google Patents
Sex identification method for chick embryo eggs Download PDFInfo
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- CN117629987A CN117629987A CN202311661323.0A CN202311661323A CN117629987A CN 117629987 A CN117629987 A CN 117629987A CN 202311661323 A CN202311661323 A CN 202311661323A CN 117629987 A CN117629987 A CN 117629987A
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- chick embryo
- eggs
- allantoic fluid
- egg
- test paper
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- 235000013601 eggs Nutrition 0.000 title claims abstract description 111
- 210000003837 chick embryo Anatomy 0.000 title claims abstract description 83
- 238000000034 method Methods 0.000 title claims abstract description 21
- 239000012530 fluid Substances 0.000 claims abstract description 37
- 238000012360 testing method Methods 0.000 claims abstract description 32
- 229940011871 estrogen Drugs 0.000 claims abstract description 30
- 239000000262 estrogen Substances 0.000 claims abstract description 30
- 238000005553 drilling Methods 0.000 claims abstract description 17
- 230000012447 hatching Effects 0.000 claims abstract description 8
- 229940088597 hormone Drugs 0.000 claims abstract description 8
- 239000005556 hormone Substances 0.000 claims abstract description 8
- 230000004044 response Effects 0.000 claims abstract description 8
- 238000001514 detection method Methods 0.000 claims abstract description 7
- 230000000249 desinfective effect Effects 0.000 claims abstract description 6
- 241000287828 Gallus gallus Species 0.000 claims description 33
- 210000001161 mammalian embryo Anatomy 0.000 claims description 19
- 239000007788 liquid Substances 0.000 claims description 13
- 239000012188 paraffin wax Substances 0.000 claims description 9
- 230000001954 sterilising effect Effects 0.000 claims description 7
- IISBACLAFKSPIT-UHFFFAOYSA-N bisphenol A Chemical compound C=1C=C(O)C=CC=1C(C)(C)C1=CC=C(O)C=C1 IISBACLAFKSPIT-UHFFFAOYSA-N 0.000 claims description 6
- 238000004140 cleaning Methods 0.000 claims description 6
- 230000005540 biological transmission Effects 0.000 claims description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 3
- 238000000605 extraction Methods 0.000 claims description 3
- 239000006260 foam Substances 0.000 claims description 3
- 238000005286 illumination Methods 0.000 claims description 3
- 230000000149 penetrating effect Effects 0.000 claims description 3
- 239000012286 potassium permanganate Substances 0.000 claims description 3
- 230000020509 sex determination Effects 0.000 claims 2
- 238000004519 manufacturing process Methods 0.000 abstract description 11
- 230000008901 benefit Effects 0.000 abstract description 6
- 238000011161 development Methods 0.000 abstract description 5
- 230000018109 developmental process Effects 0.000 abstract description 5
- 238000000926 separation method Methods 0.000 abstract description 3
- 235000013330 chicken meat Nutrition 0.000 description 20
- VOXZDWNPVJITMN-ZBRFXRBCSA-N 17β-estradiol Chemical compound OC1=CC=C2[C@H]3CC[C@](C)([C@H](CC4)O)[C@@H]4[C@@H]3CCC2=C1 VOXZDWNPVJITMN-ZBRFXRBCSA-N 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- DNXHEGUUPJUMQT-UHFFFAOYSA-N (+)-estrone Natural products OC1=CC=C2C3CCC(C)(C(CC4)=O)C4C3CCC2=C1 DNXHEGUUPJUMQT-UHFFFAOYSA-N 0.000 description 1
- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- DNXHEGUUPJUMQT-CBZIJGRNSA-N Estrone Chemical compound OC1=CC=C2[C@H]3CC[C@](C)(C(CC4)=O)[C@@H]4[C@@H]3CCC2=C1 DNXHEGUUPJUMQT-CBZIJGRNSA-N 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 235000014103 egg white Nutrition 0.000 description 1
- 210000000969 egg white Anatomy 0.000 description 1
- 230000027632 eggshell formation Effects 0.000 description 1
- 229960005309 estradiol Drugs 0.000 description 1
- 229960003399 estrone Drugs 0.000 description 1
- 230000004720 fertilization Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 150000002576 ketones Chemical class 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses a sex identification method for chick embryo eggs, which comprises the following steps: and collecting allantoic fluid of the chick embryo egg, detecting and judging the sex of the chick embryo egg by carrying out estrogen response on the allantoic fluid, disinfecting and plugging the chick embryo egg after the allantoic fluid is collected, wherein estrogen test paper is adopted for the estrogen response. According to the method, sex identification is carried out on the chick embryo in the development stage, allantoic fluid is directly sucked through drilling, the sex of the chick embryo egg is judged through the color generated by reaction of the test paper and the female hormone, so that the direct judgment of the sex of the chick embryo egg is achieved, the chick embryo eggs with different sexes are conveniently classified, the secondary nest separation process of the chick after hatching is reduced, the detection efficiency and the production benefit are effectively improved, and the sex identification is realized in the development stage of the chick embryo.
Description
Technical Field
The invention belongs to the technical field of biology, and particularly relates to a sex identification method for chick embryo eggs.
Background
The sex of chickens is closely related to its productivity. In commercial egg production, hens are the only production tools, while cocks do not have production capacity to be eliminated. In the production of fertilized eggs, the number of roosters is very small, only 3% of the total number of chickens is needed, and the remaining roosters are eliminated. In commercial broiler production, the cost of producing hen broilers is 5% higher than that of hen chickens because the hen chickens need less feed than the hen chickens, and small hen chickens can be eliminated by large-scale broiler factories. Only in 2001, 9200 ten thousand chickens were killed in Germany, reaching incredibly 2.82 hundred million and 2.26 hundred million in the United states and European Union. In China, investigation of the Beijing peripheral hen houses has found that many hen houses with hatching capability are sold after young hens are hatched, and most of the houses are killed as production raw materials. Fertilization occurs prior to eggshell formation and sex bias cannot be controlled in chicken breeding. Therefore, sex identification is carried out on chick embryo at the development stage, and chicks with different sexes are reconfigured and reasonably treated according to production requirements, so that the production benefit of the chicken industry can be greatly improved, and the ethical problem can be effectively avoided.
The sex of the chick embryo egg is identified through the estrogen content in the allantoic fluid, the chick embryo egg is drilled firstly, then the allantoic fluid of the chick embryo egg is sucked under negative pressure, however, the drilling and the suction cannot be synchronously carried out, the probability of the chick embryo egg being infected by the external environment is increased, and meanwhile, the time is consumed by adopting the estrogen centrifugal detection method for the chick embryo egg, and the cost for detecting the chick embryo egg is increased.
Therefore, there is a need to design a method for sex identification of chicken embryo eggs.
Disclosure of Invention
The invention aims to provide a sex identification method for chicken embryo eggs, which aims to solve the problems.
In order to achieve the above object, the present invention provides the following solutions:
a method for sexing chick embryo eggs, comprising the following steps:
collecting chick embryo egg allantoic fluid;
detecting the allantoic fluid by female hormone reaction to judge the sex of the embryonated eggs;
disinfecting and plugging the chick embryo eggs after the allantoic fluid is collected;
the estrogen test paper is adopted for the estrogen response.
The collection of the allantoic fluid of the chick embryo specifically comprises the following steps:
after hatching the eggs for 8 to 17 days, sterilizing the surfaces of the chick embryo laying hen eggs;
laying the chick embryo eggs on a waiting table for 30 minutes;
determining the position of allantoic fluid of an egg through illumination;
drilling holes on the allantoic fluid positions of the chick embryo eggs, and sucking 1ml of allantoic fluid in the chick embryo eggs through a drill connected with a negative pressure device after drilling holes.
The method for disinfecting and plugging the chick embryo eggs after the allantoic fluid is collected specifically comprises the following steps: and (3) sterilizing the egg holes of the chick embryo eggs again after the extraction is finished, extracting paraffin fused into liquid by a dropper, dripping the paraffin into the egg holes of the chick embryo eggs, and completely plugging the egg holes of the chick embryo eggs after the paraffin is cooled.
The detection of the allantoic fluid for female hormone response to judge the sex of the embryonated egg specifically comprises: and taking down the drill bit connected with the negative pressure device and horizontally standing, observing the color of the estrogen test paper after the drill bit horizontally stands for 5 minutes, and judging the sex of the chick embryo eggs according to the color generated by the reaction of the estrogen test paper and the estrogen.
The estrogen test paper is estrogen bisphenol A test paper.
The temperature of the operation environment is 37.8 ℃, and the chick embryo eggs hatched for 8 to 17 days are treated with 75 percent medical alcohol or 1:2500 in the presence of a potassium permanganate solution.
One side of the waiting platform is provided with a conveying frame, and the other side of the waiting platform is provided with an operating platform; the conveying frame is arranged in the cleaning tank;
the top of the operating area of the operating platform is fixedly provided with a negative pressure drill connector; the top of the negative pressure drill connector is in transmission connection with a negative pressure drilling machine, and a drill bit is arranged at the bottom of the negative pressure drill connector; the bottom surface of the operation area is fixedly provided with a foam cushion and a chick embryo egg bracket, and the bottom of the chick embryo egg bracket is also provided with a chick embryo lamp.
The drill bit comprises a drill bit main body, a liquid suction pipe is arranged in the drill bit main body in a penetrating manner, and a piston is arranged in an inner cavity of the liquid suction pipe; a push rod is fixedly arranged at one end of the piston; the push rod extends out of the inner cavity of the liquid suction pipe; a test paper groove is also formed in the drill bit main body; the liquid suction pipe is communicated with the test paper groove, and the estrogen test paper is arranged in the test paper groove.
Compared with the prior art, the invention has the following advantages and technical effects: according to the method, sex identification is carried out on the chick embryo in the development stage, allantoic fluid is directly sucked through drilling, the sex of the chick embryo egg is judged through the test paper and the color generated by the female hormone reaction, so that the direct judgment of the sex of the chick embryo egg is achieved, the chick embryo eggs with different sexes are conveniently classified, the secondary nest separation process after the chick hatching is reduced, and the detection efficiency and the production benefit are effectively improved.
Drawings
For a clearer description of an embodiment of the invention or of the solutions of the prior art, the drawings that are needed in the embodiment will be briefly described, it being obvious that the drawings in the following description are only some embodiments of the invention, and that other drawings can be obtained according to these drawings without inventive effort for a person skilled in the art:
FIG. 1 is a flow chart of the present invention;
FIG. 2 is a schematic perspective view of the present invention;
FIG. 3 is a schematic view of the structure of the console according to the present invention;
FIG. 4 is a schematic view of a transparent plastic drill bit according to the present invention;
in the figure: 1. a cleaning pool; 2. a carriage; 3. a waiting station; 4. an operation table; 5. a negative pressure drill connector; 6. a drill bit; 61. a bit body; 62. a pipette; 63. a piston; 64. a push rod; 65. a test paper groove; 7. a sponge cushion; 8. a chick embryo egg carrier; 9. chicken embryo lamp.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
In order that the above-recited objects, features and advantages of the present invention will become more readily apparent, a more particular description of the invention will be rendered by reference to the appended drawings and appended detailed description.
A method for sexing chick embryo eggs, comprising the following steps:
collecting chick embryo egg allantoic fluid;
detecting the allantoic fluid by female hormone reaction to judge the sex of the embryonated eggs;
disinfecting and plugging the chick embryo eggs after the allantoic fluid is collected;
the estrogen test strip is used for estrogen response.
The collection of the allantoic fluid of the chick embryo specifically comprises the following steps:
after hatching the eggs for 8 to 17 days, sterilizing the surfaces of the chick embryo laying hen eggs at the ambient temperature of 37.8 ℃;
laying the chick embryo eggs on a waiting table 3 for 30 minutes;
the position of the allantoic fluid of the egg is determined by illumination,
drilling holes on the allantoic fluid position of the chick embryo egg on the operation table 4, and sucking 1ml of allantoic fluid in the chick embryo egg through a drill connected with a negative pressure device after drilling holes.
In a preferred embodiment of the invention, the sex identification method of the chick embryo eggs is used for carrying out sex identification on the chick embryo eggs in the development stage of the chick embryo, directly sucking allantoic fluid through drilling, judging the sex of the chick embryo eggs through the color generated by the reaction of test paper and female hormone, so as to directly judge the sex of the chick embryo eggs, further facilitate classification of the chick embryo eggs with different sexes, reduce the secondary nest separation process after hatching of the chick, and effectively improve the detection efficiency and the production benefit.
The method for disinfecting and plugging the chick embryo eggs after the allantoic fluid is collected specifically comprises the following steps: and (3) sterilizing the egg holes of the chick embryo eggs again after the extraction is finished, extracting paraffin fused into liquid by a dropper, dripping the paraffin into the egg holes of the chick embryo eggs, and completely plugging the egg holes of the chick embryo eggs after the paraffin is cooled.
The detection of the allantoic fluid for female hormone response to determine the sex of the embryonated egg specifically comprises: and taking down the drill bit connected with the negative pressure device, horizontally standing, observing the color of the estrogen test paper after the drill bit horizontally stands for 5 minutes, and judging the sex of the chick embryo eggs according to the color generated by the reaction of the estrogen test paper and the estrogen.
The estrogen test paper is estrogen bisphenol A test paper.
The temperature of the operating environment is 37.8 ℃, and the chick embryo eggs hatched for 8 to 17 days are treated with 75 percent medical alcohol or 1:2500 in the presence of a potassium permanganate solution.
One side of the waiting platform 3 is provided with a conveying frame 2, and the other side is provided with an operating platform 4; the conveying frame 2 is arranged in the cleaning tank 1;
the top of the operating area of the operating table 4 is fixedly provided with a negative pressure drill connector 5; the top of the negative pressure drill connector 5 is in transmission connection with a negative pressure drilling machine, and the bottom of the negative pressure drill connector is provided with a drill bit 6; the bottom surface of the operation area is fixedly provided with a foam cushion 7 and a chick embryo egg bracket 8, and the bottom of the chick embryo egg bracket 8 is also provided with a chick embryo lamp 9.
In a preferred embodiment of the invention, the cleaning tank 1 is used for cleaning egg white of the chick embryo, and the conveying frame 2 is used for conveying the chick embryo eggs; the waiting table 3 is used for laying the chick embryo eggs; the operation table 4 is used for sucking allantoic fluid from the chick embryo eggs.
Further, the negative pressure drilling machine is arranged at the top of the operation table 4, the bottom is in transmission connection with the negative pressure drilling connector 5, a drill bit 6 for drilling holes on the chicken embryo eggs and sucking allantoic fluid is arranged at the lower end of the negative pressure drilling connector 5, a spongy cushion 7 for supporting the chicken embryo eggs is arranged at the table top of the operation table 4, a chicken embryo egg bracket 8 for supporting the chicken embryo eggs is further fixed on the table top of the operation table 4, a chicken embryo lamp 9 for illuminating the chicken embryo eggs is further arranged on the table top of the operation table 4, and the chicken embryo lamp 9 is located right below the chicken embryo egg bracket 8.
Further, the drill 6 is made of transparent metal.
The drill bit 6 comprises a drill bit main body 61, a liquid suction pipe 62 is arranged in the drill bit main body 61 in a penetrating way, and a piston 63 is arranged in the inner cavity of the liquid suction pipe 62; a push rod 64 is fixedly arranged at one end of the piston 63; push rod 64 extends out of the lumen of pipette 62; a test paper groove 65 is also formed in the drill bit main body 61; pipette 62 communicates with test strip well 65, and test strip well 65 is provided with estrogen test strip.
In one embodiment of the invention: hatching success rate, identification accuracy and 17 beta-estradiol content, ketone content and estrone content under different groups.
In the description of the present invention, it should be understood that the terms "longitudinal," "transverse," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like indicate or are based on the orientation or positional relationship shown in the drawings, merely to facilitate description of the present invention, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus should not be construed as limiting the present invention.
The above embodiments are only illustrative of the preferred embodiments of the present invention and are not intended to limit the scope of the present invention, and various modifications and improvements made by those skilled in the art to the technical solutions of the present invention should fall within the protection scope defined by the claims of the present invention without departing from the design spirit of the present invention.
Claims (8)
1. A method for sexing chick embryo eggs, which is characterized by comprising the following steps:
collecting chick embryo egg allantoic fluid;
detecting the allantoic fluid by female hormone reaction to judge the sex of the embryonated eggs;
disinfecting and plugging the chick embryo eggs after the allantoic fluid is collected;
the estrogen test paper is adopted for the estrogen response.
2. The method for sex determination of chicken embryo eggs according to claim 1, wherein the collection of allantoic fluid of chicken embryo eggs specifically comprises:
after hatching the eggs for 8 to 17 days, sterilizing the surfaces of the chick embryo laying hen eggs;
laying the chick embryo eggs on a waiting table (3) with the tips facing downwards and the blunt ends facing upwards for 30 minutes;
determining the position of allantoic fluid of an egg through illumination;
drilling holes on the allantoic fluid positions of the chick embryo eggs on an operation table (4), and sucking 1ml of allantoic fluid in the chick embryo eggs through a drill (6) connected with a negative pressure device after drilling holes.
3. The method for sex identification of chicken embryo eggs according to claim 1, wherein the step of sterilizing and plugging the chicken embryo eggs after the completion of allantoic fluid collection specifically comprises: and (3) sterilizing the egg holes of the chick embryo eggs again after the extraction is finished, extracting paraffin fused into liquid by a dropper, dripping the paraffin into the egg holes of the chick embryo eggs, and completely plugging the egg holes of the chick embryo eggs after the paraffin is cooled.
4. The method for sex determination of embryonated eggs according to claim 2, wherein the subjecting allantoic fluid to estrogen response detection comprises: and taking down the drill bit connected with the negative pressure device and horizontally standing, observing the color of the estrogen test paper after the drill bit horizontally stands for 5 minutes, and judging the sex of the chick embryo eggs according to the color generated by the reaction of the estrogen test paper and the estrogen.
5. The method for sexing chicken embryo eggs according to claim 1, wherein: the estrogen test paper is estrogen bisphenol A test paper.
6. The method for sexing chicken embryo eggs according to claim 2, wherein: chick embryo eggs hatched for 8 to 17 days with 75% medical alcohol or 1:2500 in the presence of a potassium permanganate solution.
7. The method for sexing chicken embryo eggs according to claim 2, wherein: one side of the waiting table (3) is provided with a conveying frame (2), and the other side of the waiting table is provided with the operating table (4); the conveying frame (2) is arranged in the cleaning tank (1);
the negative pressure device comprises a negative pressure drill connector (5) fixedly arranged at the top of an operation area of the operation table (4); the top of the negative pressure drill connector (5) is in transmission connection with a negative pressure drilling machine, and the drill bit (6) is arranged at the bottom of the negative pressure drill connector;
the bottom surface of the operation area is fixedly provided with a foam cushion (7) and a chick embryo egg bracket (8), and the bottom of the chick embryo egg bracket (8) is also provided with a chick embryo lamp (9).
8. The method for sexing chicken embryo eggs according to claim 7 wherein: the drill bit (6) comprises a drill bit main body (61), a liquid suction pipe (62) is arranged in the drill bit main body (61) in a penetrating mode, and a piston (63) is arranged in an inner cavity of the liquid suction pipe (62); a push rod (64) is fixedly arranged at one end of the piston (63); the push rod (64) extends out of the inner cavity of the liquid suction pipe (62); a test paper groove (65) is also formed in the drill bit main body (61); the liquid suction pipe (62) is communicated with the test paper groove (65), and the estrogen test paper is arranged in the test paper groove (65).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311661323.0A CN117629987A (en) | 2023-12-06 | 2023-12-06 | Sex identification method for chick embryo eggs |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311661323.0A CN117629987A (en) | 2023-12-06 | 2023-12-06 | Sex identification method for chick embryo eggs |
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| Publication Number | Publication Date |
|---|---|
| CN117629987A true CN117629987A (en) | 2024-03-01 |
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| CN202311661323.0A Pending CN117629987A (en) | 2023-12-06 | 2023-12-06 | Sex identification method for chick embryo eggs |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110230453A1 (en) * | 2008-03-11 | 2011-09-22 | Urobiologics Llc | Use of Female Mammal's Urine for Determination of Fetal Gender Related Characteristics |
| CN104704360A (en) * | 2012-07-30 | 2015-06-10 | 因奥沃私人有限公司 | Gender, viability and/or developmental stage determination of avian embryos in ovo |
| CN105191831A (en) * | 2015-09-25 | 2015-12-30 | 中国农业大学 | Method for embryonated egg sex identification |
| CN206311580U (en) * | 2016-12-28 | 2017-07-07 | 广州艾卓生物科技有限公司 | A kind of electric drill and the equipment for chick chorioallantoic membrane experiment |
| CN217221557U (en) * | 2021-12-20 | 2022-08-19 | 广西大学 | Chick embryo allantoic fluid micro-extractor |
| CN217638698U (en) * | 2022-05-10 | 2022-10-21 | 厚德食品股份有限公司 | Rapid detection device for harmless components of egg products |
-
2023
- 2023-12-06 CN CN202311661323.0A patent/CN117629987A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110230453A1 (en) * | 2008-03-11 | 2011-09-22 | Urobiologics Llc | Use of Female Mammal's Urine for Determination of Fetal Gender Related Characteristics |
| CN104704360A (en) * | 2012-07-30 | 2015-06-10 | 因奥沃私人有限公司 | Gender, viability and/or developmental stage determination of avian embryos in ovo |
| CN105191831A (en) * | 2015-09-25 | 2015-12-30 | 中国农业大学 | Method for embryonated egg sex identification |
| CN206311580U (en) * | 2016-12-28 | 2017-07-07 | 广州艾卓生物科技有限公司 | A kind of electric drill and the equipment for chick chorioallantoic membrane experiment |
| CN217221557U (en) * | 2021-12-20 | 2022-08-19 | 广西大学 | Chick embryo allantoic fluid micro-extractor |
| CN217638698U (en) * | 2022-05-10 | 2022-10-21 | 厚德食品股份有限公司 | Rapid detection device for harmless components of egg products |
Non-Patent Citations (2)
| Title |
|---|
| 王健 , 漠纵泉 , 肖小 , 刘长国 , 刘向萍 , 王晓峰: "鸡胚性别鉴定――家禽科学研究的新视点", 中国家禽, no. 14, 25 July 2003 (2003-07-25), pages 23 - 33 * |
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