CN117625366B - Feeding use Complex bacteria method for preparing culture - Google Patents

Feeding use Complex bacteria method for preparing culture Download PDF

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CN117625366B
CN117625366B CN202410097068.XA CN202410097068A CN117625366B CN 117625366 B CN117625366 B CN 117625366B CN 202410097068 A CN202410097068 A CN 202410097068A CN 117625366 B CN117625366 B CN 117625366B
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fermentation
plate
cavity
material turning
bacteria culture
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CN117625366A (en
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刘大程
刘世雄
李薛强
徐子萱
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Inner Mongolia Agricultural University
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Inner Mongolia Agricultural University
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87Re-use of by-products of food processing for fodder production

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Abstract

The invention relates to the technical field of preparation of composite bacteria for feeding, in particular to a preparation method of a composite bacteria culture for feeding, and also in particular relates to a turning fermentation device of the composite bacteria culture for feeding, the equipment comprises a double-cavity fermentation chamber, wherein two cavities of the double-cavity fermentation chamber are internally provided with material turning units, and each material turning unit consists of an inner stirring piece, an outer stirring piece, an upper stirring piece and a lower stirring piece. According to the characteristics of the strain and the metabolite, a constant temperature fermentation device with two chambers for synchronous and independent fermentation and novel automatic fermentation function capable of intermittently and reciprocally turning materials is designed, the automation degree is high, the operation manpower is greatly reduced, the working efficiency is effectively improved, and the fermentation time is shortened; the material turning unit has the function of performing reciprocating material turning on fermentation materials in different directions, performs reciprocating material turning on piled materials from inside to outside in the horizontal direction through the inner and outer stirring pieces, and performs reciprocating material turning on materials in the vertical direction through the upper and lower stirring pieces.

Description

Feeding use Complex bacteria method for preparing culture
Technical Field
The invention relates to the technical field of preparation of composite bacteria for feeding, in particular to a preparation method of a composite bacteria culture for feeding.
Background
Problems of dysbacteriosis, increased drug-resistant strains, drug residues of animal products and the like caused by overuse of antibiotics are increasingly prominent in recent years, and research on antibiotic substitutes becomes a hotspot field; at present, various replacing resistant products mainly comprise a microecological preparation, an enzyme preparation, a Chinese herbal medicine preparation, a functional oligosaccharide, an acidulant, a fermented feed and the like, wherein the microbial fermented feed shows positive effects, but the quality stability of the products is poor and the homogenization of the products is serious, mainly due to the fact that the fermentation process is not mature, and the existing microbial fermented feed fermentation process has the following defects: 1. at present, most biological fermentation manufacturers adopt bagged fermentation to reduce the production cost, and the method is favorable for anaerobic fermentation and unfavorable for aerobic bacteria, so that the product quality is influenced; 2. for fermentation in a fermentation tank, the optimal fermentation process cannot be completely selected according to the characteristics of strains and the characteristics of metabolite products; 3. the material turning direction given by the material turning mechanism is fixed and single, the material turning effect can be achieved in a given time, and the piled materials are difficult to be mixed uniformly effectively, so that the quality of the fermented product is not uniform and the fermentation efficiency can be directly influenced; 4. the material quantity of single fermentation is limited, so that the overall efficiency of the preparation of the composite bacteria culture for feeding is affected to a certain extent, and therefore, a novel fermentation preparation process adapting to the characteristics of composite bacteria strains and the characteristics of metabolites is urgently needed.
Disclosure of Invention
In order to solve the technical problems, the invention provides a preparation method of a composite bacteria culture for feeding, which comprises the following steps: s1, reviving saccharomycetes: taking out two strains of high-activity saccharomycetes from a refrigerator at the temperature of minus 80 ℃ and according to the mass ratio of 1:1, and then reviving the strain by a wort culture medium to obtain the required original bacterial liquid.
S2, enrichment culture of bacterial liquid: mixing 8-10% of original bacterial liquid, 1-1.5% of organic fertilizer fermentation liquor, 6-7% of molasses, 0.1-0.2% of yeast extract, 1-1.5% of glucose and 81-82% of water according to the formula mass ratio, and placing the mixture in a constant temperature shaking table for enrichment culture.
S3, preparing a solid fermentation medium: the solid fermentation culture medium is prepared according to the formula mass ratio of 10-15% of soybean meal, 15-18% of corn, 10-15% of malt meal, 10-12% of tryptone, 15-18% of corn germ meal, 3-5% of puffed soybean, 16-18% of corn protein powder and 5-8% of peptone.
S4, inoculating strains and adjusting the water content: inoculating the bacterial liquid activated in the step S2 into the solid-state fermentation culture medium prepared in the step S3 according to the mass ratio of 10% of inoculum size, so that the initial moisture content of the solid-state fermentation culture medium reaches 40-41%.
S5, solid-state stacking fermentation: and (3) placing the solid fermentation culture medium subjected to strain inoculation and water content adjustment in the step (S4) into a material turning fermentation device through a feed inlet (3) for solid stacking fermentation, wherein the stacking height is 60-65cm, the fermentation time is 72 hours, the material temperature is recorded every 3 hours in the fermentation process, if the temperature reaches more than 40 ℃, the material is turned through the material turning fermentation device, the stacking fermentation is continued after the material turning is finished, and the fermentation is finished after 72 hours.
S6, low-temperature drying, crushing and mixing: and (3) drying the materials subjected to the solid-state stacking fermentation in the step (S5) at a low temperature of 45-50 ℃, crushing the materials after the drying, and mixing the crushed materials according to the formula mass ratio of 97-99% and 1-3% of the nutrition package to obtain the required composite bacteria culture for feeding.
S7, packaging and stacking: and (3) packaging and stacking the feeding compound bacteria culture obtained in the step (S6).
The preparation method of the composite bacteria culture for feeding in the steps S1-S7 further specifically relates to a material turning fermentation device for the composite bacteria culture for feeding, the device comprises a double-cavity fermentation chamber, material turning units are arranged in two cavities of the double-cavity fermentation chamber, a feed inlet is arranged in each cavity, and a sealing cover body is arranged at the upper ends of the two cavities.
The material turning unit comprises an inner stirring piece, an outer stirring piece and an up-down stirring piece, wherein the inner stirring piece comprises a top cover with a central shaft arranged in the center, the top cover is rotatably arranged at the lower end of a sealing cover body, vertical rods are arranged on two sides of the central shaft in a symmetrical mode, the vertical rods are connected through toothed plates which are slidably arranged at the lower end of the top cover, the upper end of each vertical rod slidably penetrates through the top cover, the upper end of one vertical rod is connected with a pushing end of a hydraulic rod arranged at the upper end of the top cover through a connecting plate, and the two central shafts are rotatably controlled by the same driving piece.
The vertical turning piece comprises a vertical frame, the vertical frame is installed to the center pin outer wall, the inside groove has been seted up to vertical frame upper end, a plurality of horizontal poles are all installed on the built-in board from last down slidable mounting to the one end of center pin back to vertical frame, and the horizontal pole runs through behind the vertical frame, and the built-in board is vertical to slide to be set up and slide and run through the top cap, and built-in board upper end lateral wall is provided with tooth group, and tooth group is connected with the pinion meshing, and the pinion lower extreme meshing is connected with the master gear, and master gear and pinion are installed on same axostylus axostyle, and the axostylus axostyle passes through the pole seat rotation and installs at the top cap lower extreme.
In a possible implementation mode, the material turning fermentation equipment further comprises a discharging piece, wherein the discharging piece is used for synchronously controlling the discharging of the two cavities, the bottom walls of the two cavities are concave surfaces which are gradually concave towards the center, a discharging through groove is formed in the center of the concave surface, the discharging piece comprises a mounting plate located below the double-cavity fermentation chamber, a cylinder is mounted at the lower end of the mounting plate, and a plugging block matched with the corresponding falling through groove in a plugging manner is mounted at the upper end of the mounting plate.
In one possible implementation mode, annular grooves concentric with the plugging blocks are formed in the positions, corresponding to the cavities, of the lower end surfaces of the double-cavity fermentation chambers, and heating pipelines are arranged in the annular grooves.
In one possible implementation mode, the built-in plates are symmetrically arranged, one built-in plate is in sliding connection with the inner groove, the other built-in plate is arranged in the side scraping plate in a sliding mode, the outer side wall of the side scraping plate is in sliding fit with the inner annular wall of the cavity, and the lower end of the side scraping plate is connected with the vertical frame through the butt joint plate.
In one possible implementation manner, a lower plate for bottom stirring and scraping the bottom wall of the cavity is mounted at the lower end of the central shaft, and the lower end of the lower plate is of an arc-shaped structure attached to the bottom wall of the cavity.
In one possible implementation mode, a plurality of cross bars which are equidistantly distributed from top to bottom are connected between the vertical bars, and the cross bars horizontally slide to penetrate through the central shaft.
In one possible implementation manner, the lower end of the vertical rod is provided with a U-like plate, and the U-like plate is in sliding connection with the lower plate and is used for scraping the outer side wall of the lower plate.
In one possible implementation mode, the outer wall of the central shaft is symmetrically provided with a conical sleeve corresponding to the position of the cross rod, the cross rod penetrates through the conical sleeve in a sliding mode, and the inner diameter of the small-caliber end of the conical sleeve is equal to the outer diameter of the cross rod.
In one possible implementation manner, a plurality of U-shaped cleaning plates corresponding to the ends of the horizontal rods are connected to the vertical frame and the side scraping plates in a sliding manner.
The beneficial effects are that: 1. according to the strain characteristics and metabolite characteristics, the invention designs the constant-temperature fermentation device with the novel automatic fermentation function, wherein the two cavities synchronously and independently perform fermentation and can perform reciprocating intermittent material turning, the degree of automation is high, the operation manpower is greatly reduced, the working efficiency is effectively improved, the fermentation time is shortened, and the constant-temperature fermentation device is suitable for anaerobic and facultative fermentation and can meet the requirement of product diversification of production lines.
2. The material turning unit has the function of carrying out reciprocating material turning on fermentation materials in different directions, horizontally and downwards carries out reciprocating material turning from inside to outside on piled materials through the inner and outer stirring pieces, so that the center and the outer materials are uniformly mixed, vertically and downwards carries out reciprocating material turning through the upper and lower stirring pieces, the bottom and the upper materials are uniformly mixed, and circumferential stirring material turning is synchronously formed on the basis of the inner and outer material turning and the upper and lower material turning, thereby fully and effectively carrying out multi-state reciprocating material turning on the materials through the cooperation of the inner and outer stirring pieces and the upper and lower stirring pieces, greatly improving the uniform effect of material mixing, improving the quality of products and ensuring the uniformity and stability of final products.
3. The circumferential stirring and turning of the two inner and outer stirring pieces are controlled by the same driving source, and the inner and outer stirring pieces and the up and down stirring pieces in the single cavity are matched to realize inner and outer and up and down reciprocating turning which is also controlled by the single driving source, so that the degree of automation is improved on the basis of reducing the number of the driving sources and increasing the turning synchronism.
4. The invention realizes the synchronous multi-position scraping and cleaning function while carrying out inner and outer and up-and-down reciprocating material turning and circumferential stirring material turning, does not need an additional driving source in the scraping and cleaning operation, greatly reduces the residual quantity of discharged materials by utilizing the synchronous multi-position scraping and cleaning function, completes basic cleaning while discharging, does not need frequent cleaning, and greatly reduces the residual quantity of discharged materials, thereby being beneficial to avoiding material contamination and improving the fermentation quality.
Drawings
Fig. 1 is a process flow diagram of the present invention.
Fig. 2 is a schematic perspective view of the present invention.
FIG. 3 is a schematic perspective view of a material turning unit and a partially sectioned double-cavity fermentation chamber of the present invention.
Fig. 4 is an enlarged schematic view of the present invention at the X area in fig. 3.
Fig. 5 is an enlarged schematic view of the Y region of fig. 3 according to the present invention.
Fig. 6 is an enlarged schematic view of the Z region of fig. 3 in accordance with the present invention.
Fig. 7 is a schematic view in front cross-section of fig. 2 of the present invention.
Fig. 8 is a schematic view of a partial perspective view of a cross bar, cone sleeve, and vertical bar of the present invention.
In the figure: 1. a double-cavity fermentation chamber; 2. a turning unit; 3. a feed inlet; 4. a cover body; 5. a discharging piece; 210. a central shaft; 211. a top cover; 212. a vertical rod; 213. a toothed plate; 214. a hydraulic rod; 215. a driving member; 216. a lower plate; 217. a cross bar; 218. a U-like plate; 219. a cone sleeve; 220. a vertical frame; 221. a horizontal bar; 222. a built-in plate; 223. a pinion gear; 224. a shaft lever; 225. a side scraper; 226. a cleaning plate; 50. a mounting plate; 51. a block; 52. a ring groove; 53. a heating pipe; 54. an electric heating rod; 55. a sealing plate; 56. and a cover plate.
Detailed Description
In order that the above objects, features and advantages of the invention will be readily understood, a more particular description of the invention will be rendered by reference to the appended drawings. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. The invention may be embodied in many other forms than described below and similarly modified by those skilled in the art without departing from the spirit or scope of the invention, which is therefore not limited to the specific embodiments disclosed below.
Referring to fig. 1 and 2, a preparation method of a feeding composite bacteria culture includes the following steps: s1, reviving saccharomycetes: taking out two strains of high-activity saccharomycetes from a refrigerator at the temperature of minus 80 ℃ and according to the mass ratio of 1:1, and then reviving the strain by a wort culture medium to obtain the required original bacterial liquid.
S2, enrichment culture of bacterial liquid: mixing 8-10% of original bacterial liquid, 1-1.5% of organic fertilizer fermentation liquor, 6-7% of molasses, 0.1-0.2% of yeast extract, 1-1.5% of glucose and 81-82% of water according to the formula mass ratio, and placing the mixture in a constant temperature shaking table for enrichment culture; wherein the organic fertilizer fermentation liquor is prepared by fermenting organic waste and microorganisms in soil, and is widely used in agricultural production, also called liquid microbial inoculant.
S3, preparing a solid fermentation medium: the solid fermentation culture medium is prepared according to the formula mass ratio of 10-15% of soybean meal, 15-18% of corn, 10-15% of malt meal, 10-12% of tryptone, 15-18% of corn germ meal, 3-5% of puffed soybean, 16-18% of corn protein powder and 5-8% of peptone.
S4, inoculating strains and adjusting the water content: inoculating the bacterial liquid activated in the step S2 into the solid-state fermentation culture medium prepared in the step S3 according to the mass ratio of 10% of inoculum size, so that the initial moisture content of the solid-state fermentation culture medium reaches 40-41%.
S5, solid-state stacking fermentation: and (3) placing the solid fermentation culture medium subjected to strain inoculation and water content adjustment in the step (S4) into a material turning fermentation device through a feed inlet (3) for solid stacking fermentation, wherein the stacking height is 60-65cm, the fermentation time is 72 hours, the material temperature is recorded every 3 hours in the fermentation process, if the temperature reaches more than 40 ℃, the material is turned through the material turning fermentation device, the stacking fermentation is continued after the material turning is finished, and the fermentation is finished after 72 hours.
S6, low-temperature drying, crushing and mixing: drying the materials subjected to solid-state stacking fermentation in the step S5 at a low temperature of 45-50 ℃, crushing the materials after drying, and mixing the crushed materials according to the formula mass ratio of 97-99% and 1-3% of nutrition bags to obtain a required composite bacteria culture for feeding; wherein the nutritional bag is prepared by mixing one or more of carbon source, nitrogen source, mineral element, trace element, etc., and is used for promoting growth and metabolism of microorganism, bacteria or fungi.
S7, packaging and stacking: and (3) packaging and stacking the feeding compound bacteria culture obtained in the step (S6).
The productivity of the feed composite bacteria culture of each ton of raw materials in the traditional feed composite bacteria culture preparation process is 90.24%, and the production efficiency of the feed composite bacteria culture of each ton of raw materials is 98.48% by designing constant-temperature turning fermentation equipment in the feed composite bacteria culture preparation process, which is improved by 8.24% compared with the traditional feed composite bacteria culture preparation process; compared with the composite bacteria culture for feed prepared by the traditional preparation process, the composite bacteria culture for feed prepared by the preparation process of the patent application has the advantages that the content of active ingredients is obviously improved, wherein the content of organic acid is improved to 1.53mg/g from 0.65mg/g, the content of polypeptide is improved to 1.59mg/g from 0.56mg/g, the content of beta-glucan is improved to 10.56mg/g from 5.65mg/g, and the content of mannans is improved to 8.79mg/g from 4.69 mg/g; compared with the feeding composite bacteria culture prepared by the traditional preparation process, the feeding composite bacteria culture prepared by the preparation process of the patent application has the advantages that the yield and the content of active ingredients are effectively improved, and the cost is greatly reduced.
The organic acid can be used as an acidulant in the feed to improve the intestinal environment of animals, and a proper amount of the organic acid can reduce the pH value of the intestinal tract, prevent the growth of harmful bacteria and promote the growth and proliferation of probiotics, so that the digestion and absorption of the feed are promoted; the polypeptide is formed by connecting amino acids through peptide bonds, has a certain nutrition function, and a proper amount of polypeptide can improve the protein content of the feed, provide necessary amino acids for animals, and is beneficial to promoting the growth and the development and improving the nutrition value of the feed; the beta-glucan is used as a polysaccharide substance, is helpful for enhancing the immunity of animals in feed, has a certain effect on antibiosis and antivirus, and the proper beta-glucan content can promote the immune function of the animals; the mannans have good water retention and viscosity, have positive effects on the regulation of the intestinal functions of animals, and a proper amount of mannans are beneficial to promoting the balance of intestinal flora and enhancing the stability of feed, so that compared with the feeding composite bacterial culture prepared by the traditional preparation process, the feeding composite bacterial culture prepared by the preparation process has the advantages that the digestion capacity of animals can be improved, the growth of the animals is promoted, the immunity and the regulation direction of the intestinal functions are effectively improved, and the feeding composite bacterial culture has remarkable effects.
Referring to fig. 2 and 3, in the process of the preparation method of the feeding composite bacterial culture by adopting the steps S1 to S7, the invention further specifically relates to a feeding composite bacterial culture turning fermentation device, which comprises: the double-cavity fermentation chamber 1, all be provided with in two cavitys of double-cavity fermentation chamber 1 and turn over material unit 2, all be provided with feed inlet 3 on two cavitys and two cavitys upper end are provided with closing cap body 4 jointly, and two cavitys of double-cavity fermentation chamber 1 all are provided with the control by temperature change inductor (not shown in the figure).
Referring to fig. 2, 3, 4, 5 and 6, the material turning unit 2 is composed of an inner and outer stirring member and an up and down stirring member, the inner and outer stirring member includes a top cover 211 with a central shaft 210 installed in the center, the top cover 211 is rotatably installed at the lower end of the cover body 4, vertical rods 212 are respectively provided at two symmetrical sides of the central shaft 210, the vertical rods 212 are connected through a toothed plate 213 slidably installed at the lower end of the top cover 211, the upper end of one vertical rod 212 slidably penetrates through the top cover 211, the upper end of one vertical rod 212 is connected with the pushing end of a hydraulic rod 214 installed at the upper end of the top cover 211 through a connecting plate, the two central shafts 210 are rotatably controlled by the same driving member 215, and the driving member 215 includes a belt connected between the two central shafts 210 and a motor connected with the middle part of the belt and installed in the cover body 4; the lower end of the central shaft 210 is fitted with a lower plate 216 for bottom agitation and scraping of the bottom wall of the chamber.
Referring to fig. 3, 4 and 5, the flip-up and flip-down component includes a vertical frame 220, a vertical frame 220 is mounted on the outer wall of the central shaft 210, an inner groove is formed at the upper end of the vertical frame 220, a plurality of horizontal rods 221 are slidably mounted at one end of the vertical frame 220 opposite to the central shaft 210 from top to bottom, the horizontal rods 221 are all mounted on a built-in plate 222 after penetrating through the vertical frame 220, the built-in plate 222 is vertically slidably disposed and slidably penetrates through the top cover 211, a tooth set is disposed on the side wall of the upper end of the built-in plate 222, the tooth set is in meshed connection with a pinion 223, a main gear is connected with the lower end of the tooth plate 213 in meshed connection with the pinion 223, the main gear and the pinion 223 are mounted on the same shaft 224, and the shaft 224 is rotatably mounted at the lower end of the top cover 211 through a rod seat; the built-in plates 222 are symmetrically arranged, one built-in plate 222 is in sliding connection with the inner groove, the other built-in plate 222 is arranged in the side scraping plate 225 in a sliding mode, the outer side wall of the side scraping plate 225 is in sliding fit with the inner annular wall of the cavity, and the lower end of the side scraping plate 225 is connected with the vertical frame 220 through an abutting plate. The symmetrical arrangement of the built-in plates 222 can effectively improve the stability of the vertical movement of the horizontal rod 221 and the circumferential rotation of the side scraping plates 225, thereby improving the mixing uniformity of materials.
Solid-state stacking fermentation process: when the temperature reaches above 40 ℃, the material turning unit 2 starts to operate, the central shaft 210 and the top cover 211 are driven to synchronously rotate through the cooperation of a motor and a belt, the vertical rods 212 synchronously rotate, the rotating vertical rods 212 circumferentially agitate materials in the cavity to uniformly mix the materials, meanwhile, the two vertical rods 212 connected with the toothed plate 213 are pushed by the hydraulic rods 214 and the connecting plate to do reciprocating horizontal linear movement along the radial direction of the top cover 211, the vertical rods 212 in the reciprocating horizontal linear movement are utilized to stir the materials from inside to outside, the uniform material mixing effect is further improved, the toothed plate 213 is in the reciprocating horizontal linear movement process, the main gear meshed with the toothed plate synchronously does reciprocating positive and negative rotation, the main gear drives the auxiliary gear 223 to synchronously rotate through the shaft rod 224, then the toothed group drives the horizontal rod 221 to reciprocate up and down through the built-in plate 222, the horizontal rod 221 vertically reciprocates to uniformly mix the materials from top to bottom, and uniform and stable final products are ensured. During the multi-directional and multi-state stirring of the materials, the central shaft 210 drives the lower plate 216 to stir the materials adhered to the bottom wall of the cavity and simultaneously scrape the bottom wall of the cavity in real time so as to avoid the influence of the adhesion of the materials on the bottom wall of the cavity to form a stacking layer on the mixing, fermentation and subsequent discharging of the materials, and meanwhile, the side scraping plate 225 scrapes the inner annular wall of the cavity along with the circumferential rotation of the central shaft 210 and further performs effective stirring and mixing on the materials adhered to the inner annular wall of the cavity in cooperation with the vertical rods 212.
Referring to fig. 2, 3 and 7, the material turning fermentation apparatus further includes a material discharging member 5 for synchronously controlling the material discharging of the two cavities, the bottom walls of the two cavities are concave surfaces gradually recessed toward the center, a material discharging through groove is formed in the center of the concave surface, the material discharging member 5 includes a mounting plate 50 located below the double-cavity fermentation chamber 1, a cylinder is mounted at the lower end of the mounting plate 50, and a plugging block 51 in plug-in fit with the corresponding falling through groove is mounted at the upper end of the mounting plate 50. The lower end of the lower plate 216 is an arc structure attached to the bottom wall of the cavity, so as to ensure the scraping effect of the lower plate 216 on the bottom wall of the cavity.
After the material in the cavity is fermented, the mounting plate 50 and the blocking block 51 are driven to move downwards through the air cylinder, so that the blocking block 51 is separated from the blanking through groove, the fermented material falls from the blanking through groove along the inner concave surface, the material is quickly and fully blanked through the existing storage equipment, during blanking, the motor pauses to work, the vertical rod 212 keeps reciprocating horizontal movement and the horizontal rod 221 moves up and down, the material blanking speed is accelerated, and during the material blanking rear course, the motor is operated again to enable the lower plate 216 and the side scraping plate 225 to rotate, so that the material is fully blanked, and meanwhile, the inner wall of the cavity is cleaned. And (3) drying the collected materials at a low temperature after blanking, crushing the materials after drying, and mixing the crushed materials with a nutrition bag according to a certain proportion to obtain the required composite bacteria culture for feeding.
Referring to fig. 3, 7 and 8, ring grooves 52 concentric with the plugging blocks 51 are provided on the lower end surface of the double-cavity fermentation chamber 1 corresponding to the cavity, heating pipes 53 are provided in the ring grooves 52, and during material fermentation, fermentation temperature is flexibly controlled by the provided heating pipes 53 in cooperation with a temperature sensor. In order to further make the overall fermentation temperature of the solid fermentation medium uniform, an inner hole is formed in the middle of the vertical rod 212, and an electric heating rod 54 is arranged in the inner hole. In order to facilitate the maintenance of the heating pipeline 53 and the electric heating rod 54, the lower end of the ring groove 52 is opened and is sealed by a sealing plate 55, and the sealing plate 55 is arranged at the lower end of the double-cavity fermentation chamber 1 by bolts; the screw cap is arranged at the upper end of the vertical rod 212 in a threaded manner, meanwhile, the cover plate 56 is arranged at the upper end of the cover body 4 in a threaded manner at the position corresponding to the cavity, and the heating pipeline 53 and the electric heating rod 54 can be conveniently inspected by disassembling the bolts and the sealing plate 55 and the cover plate 56 and the screw cap.
Referring to fig. 3, 5 and 6, a plurality of cross bars 217 are connected between the vertical bars 212, and the cross bars 217 horizontally slide through the central shaft 210. In the process of circumferential rotation and reciprocating horizontal linear movement of the vertical rods 212, the cross rods 217 are driven to synchronously move, the range of circumferential stirring and mixing is enlarged by utilizing the cross rods 217, the uniform mixing effect of materials is further improved, the fermentation time is shortened, and the fermentation efficiency is improved.
Referring to fig. 6, a U-like plate 218 is mounted on the lower end of the vertical rod 212, and the U-like plate 218 is slidably connected to the lower plate 216 and is used for scraping the outer side wall of the lower plate 216. In the process that the vertical rods 212 drive the U-shaped plate 218 to synchronously reciprocate horizontally and linearly, the U-shaped plate 218 synchronously scrapes the outer side wall of the lower plate 216, so that the scraping effect of the lower plate 216 is prevented from being influenced due to the fact that the scraped material of the lower plate 216 is attached to the lower plate 216, namely, the scraping effect of the lower plate 216 is ensured through the scraping operation of the U-shaped plate 218.
Referring to fig. 6 and 8, the outer wall of the central shaft 210 is symmetrically provided with a tapered sleeve 219 corresponding to the position of the cross rod 217, the cross rod 217 penetrates through the tapered sleeve 219 in a sliding manner, and the inner diameter of the small-caliber end of the tapered sleeve 219 is equal to the outer diameter of the cross rod 217. In the process of reciprocating horizontal linear movement of the cross rod 217, scraping operation is carried out on the surface of the cross rod 217 by using the conical sleeve 219, so that the whole movement of the cross rod 217 and the vertical rod 212 is smooth, and the scraped material is prevented from being accumulated between the conical sleeve 219 and the outer wall of the central shaft 210 by using the appearance characteristics of the conical sleeve 219.
Referring to fig. 4 and 5, a plurality of U-shaped cleaning plates 226 are slidably connected to the vertical frame 220 and the side scraping plates 225, which correspond to the ends of the horizontal bars 221. The horizontal bar 221 moves up and down and drives the cleaning plate 226 to move along the vertical frame 220 and the side scraping plate 225, and scraping cleaning is performed on the outer walls of the vertical frame 220 and the side scraping plate 225 through the cleaning plate 226.
In the description of the present invention, it should also be noted that, unless explicitly specified and limited otherwise, the terms "disposed," "connected," "mounted," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, integrally connected, or slidably connected; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present invention will be understood in specific cases by those of ordinary skill in the art.
The embodiments of the present invention are all preferred embodiments of the present invention, and are not intended to limit the scope of the present invention in this way, therefore: all equivalent changes in structure, shape and principle according to the present invention should be covered in the protection scope of the present invention.

Claims (7)

1. The material turning fermentation equipment for the composite bacteria culture for feeding is characterized by comprising a double-cavity fermentation chamber (1), wherein material turning units (2) are arranged in two cavities of the double-cavity fermentation chamber (1), a plurality of feed inlets (3) are arranged on the two cavities, and a sealing cover body (4) is arranged at the upper ends of the two cavities;
the material turning unit (2) consists of an inner stirring piece, an outer stirring piece and an up-down stirring piece, wherein the inner stirring piece and the outer stirring piece comprise a top cover (211) with a central shaft (210) at the center, the top cover (211) is rotatably arranged at the lower end of a sealing cover body (4), vertical rods (212) are respectively arranged at two symmetrical sides of the central shaft (210), the vertical rods (212) are connected through toothed plates (213) which are slidably arranged at the lower end of the top cover (211), the upper end of each vertical rod (212) penetrates through the top cover (211) in a sliding manner, the upper end of one vertical rod (212) is connected with the pushing end of a hydraulic rod (214) arranged at the upper end of the top cover (211) through a connecting plate, and the two central shafts (210) are rotatably controlled by the same driving piece (215); a plurality of cross bars (217) which are equidistantly distributed from top to bottom are connected between the vertical bars (212), and the cross bars (217) horizontally slide to penetrate through the central shaft (210);
the up-down turning piece comprises a vertical frame (220), the outer wall of a central shaft (210) is provided with the vertical frame (220), the upper end of the vertical frame (220) is provided with an inner groove, one end of the vertical frame (220) opposite to the central shaft (210) is provided with a plurality of horizontal rods (221) in a sliding manner from top to bottom, the horizontal rods (221) penetrate through the vertical frame (220) and then are arranged on a built-in plate (222), the built-in plate (222) is vertically arranged in a sliding manner and penetrates through a top cover (211) in a sliding manner, the side wall of the upper end of the built-in plate (222) is provided with a tooth group, the tooth group is in meshed connection with a pinion (223), the lower end of the tooth plate (213) is in meshed connection with a main gear, the main gear and the pinion (223) are arranged on the same shaft lever (224), and the shaft lever (224) is rotatably arranged at the lower end of the top cover (211) through a lever seat;
the built-in plates (222) are symmetrically arranged, one built-in plate (222) is in sliding connection with the inner groove, the other built-in plate (222) is arranged in the side scraping plate (225) in a sliding mode, the outer side wall of the side scraping plate (225) is in sliding fit with the inner annular wall of the cavity, and the lower end of the side scraping plate (225) is connected with the vertical frame (220) through an abutting plate.
2. The apparatus for the fermentation of a feed complex bacteria culture according to claim 1, wherein: the material turning fermentation equipment further comprises a discharging piece (5) for synchronously controlling the discharging of the two cavities, the bottom walls of the two cavities are concave surfaces gradually towards the center, a discharging through groove is formed in the center of the concave surface, the discharging piece (5) comprises a mounting plate (50) located below the double-cavity fermentation chamber (1), a cylinder is mounted at the lower end of the mounting plate (50), and a plugging block (51) in plug connection with the corresponding falling through groove is mounted at the upper end of the mounting plate (50).
3. The apparatus for the fermentation of a feed complex bacteria culture according to claim 2, wherein: annular grooves (52) concentric with the plugging blocks (51) are formed in the positions, corresponding to the cavities, of the lower end surfaces of the double-cavity fermentation chambers (1), and heating pipelines (53) are arranged in the annular grooves (52).
4. The apparatus for the fermentation of a feed complex bacteria culture according to claim 1, wherein: the lower end of the central shaft (210) is provided with a lower plate (216) for stirring the bottom and scraping the bottom wall of the cavity, and the lower end of the lower plate (216) is of an arc-shaped structure attached to the bottom wall of the cavity.
5. The apparatus for the fermentation of a feed complex bacteria culture according to claim 4, wherein: the lower end of the vertical rod (212) is provided with a U-like plate (218), and the U-like plate (218) is in sliding connection with the lower plate (216) and is used for scraping the outer side wall of the lower plate (216).
6. The apparatus for the fermentation of a feed complex bacteria culture according to claim 1, wherein: conical sleeves (219) are symmetrically arranged on the outer wall of the central shaft (210) corresponding to the cross rods (217), the cross rods (217) penetrate through the conical sleeves (219) in a sliding mode, and the inner diameter of the small-caliber end of each conical sleeve (219) is equal to the outer diameter of each cross rod (217).
7. The apparatus for the fermentation of a feed complex bacteria culture according to claim 1, wherein: a plurality of U-shaped cleaning plates (226) which are in one-to-one correspondence with the ends of the horizontal rods (221) are connected to the vertical frame (220) and the side scraping plates (225) in a sliding mode.
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Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20030095441A (en) * 2002-06-10 2003-12-24 네오바이오다임 주식회사 Solid state fermenter of mushroom or microorganism, coupled asceptic packing mechanism and fermentation method
CN111334407A (en) * 2020-03-16 2020-06-26 高唐华农生物工程有限公司 Process technology and process equipment for preparing yeast culture by deep fermentation of yeast
CN111471578A (en) * 2020-04-15 2020-07-31 奥格生物技术(六安)有限公司 Full-automatic heap of biological fermentation fodder turns over device
CN111849743A (en) * 2020-07-30 2020-10-30 安徽牧仕达饲料科技有限公司 Premixed feed fermentation device and using method thereof
CN215279117U (en) * 2020-12-08 2021-12-24 湖南省土壤肥料研究所 Stirring fermentation device capable of turning materials up and down
CN215947273U (en) * 2021-10-28 2022-03-04 宁波中瑞生物科技有限公司 Automatic material turning device for microbial fermentation feed
CN216513886U (en) * 2021-12-27 2022-05-13 山东省明发同茂饲料有限公司 Solid state fermentation fodder scale production equipment
CN217895625U (en) * 2022-07-04 2022-11-25 安徽宝杰生物科技有限公司 Full-automatic control by temperature change stirring device of fermented feed
CN217948094U (en) * 2022-08-24 2022-12-02 江苏优仕生物科技发展有限公司 Biological fermentation feed production equipment for pigs
CN218146604U (en) * 2022-08-29 2022-12-27 青岛新康生物科技有限公司 Feed fermentation equipment for feeding meat ducks
CN116875427A (en) * 2023-09-07 2023-10-13 格莱德(福建)生物科技有限公司 Fermenting installation is used in fodder processing

Patent Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20030095441A (en) * 2002-06-10 2003-12-24 네오바이오다임 주식회사 Solid state fermenter of mushroom or microorganism, coupled asceptic packing mechanism and fermentation method
CN111334407A (en) * 2020-03-16 2020-06-26 高唐华农生物工程有限公司 Process technology and process equipment for preparing yeast culture by deep fermentation of yeast
CN111471578A (en) * 2020-04-15 2020-07-31 奥格生物技术(六安)有限公司 Full-automatic heap of biological fermentation fodder turns over device
CN111849743A (en) * 2020-07-30 2020-10-30 安徽牧仕达饲料科技有限公司 Premixed feed fermentation device and using method thereof
CN215279117U (en) * 2020-12-08 2021-12-24 湖南省土壤肥料研究所 Stirring fermentation device capable of turning materials up and down
CN215947273U (en) * 2021-10-28 2022-03-04 宁波中瑞生物科技有限公司 Automatic material turning device for microbial fermentation feed
CN216513886U (en) * 2021-12-27 2022-05-13 山东省明发同茂饲料有限公司 Solid state fermentation fodder scale production equipment
CN217895625U (en) * 2022-07-04 2022-11-25 安徽宝杰生物科技有限公司 Full-automatic control by temperature change stirring device of fermented feed
CN217948094U (en) * 2022-08-24 2022-12-02 江苏优仕生物科技发展有限公司 Biological fermentation feed production equipment for pigs
CN218146604U (en) * 2022-08-29 2022-12-27 青岛新康生物科技有限公司 Feed fermentation equipment for feeding meat ducks
CN116875427A (en) * 2023-09-07 2023-10-13 格莱德(福建)生物科技有限公司 Fermenting installation is used in fodder processing

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
刘遂俊.一步到位精修电动三轮车.机械工业出版社,2022,(第2版),第44页. *

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