CN117604070A - Portable test strip for safely detecting hyperuricemia - Google Patents
Portable test strip for safely detecting hyperuricemia Download PDFInfo
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- CN117604070A CN117604070A CN202311567293.7A CN202311567293A CN117604070A CN 117604070 A CN117604070 A CN 117604070A CN 202311567293 A CN202311567293 A CN 202311567293A CN 117604070 A CN117604070 A CN 117604070A
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- 238000012360 testing method Methods 0.000 title claims abstract description 35
- 201000001431 Hyperuricemia Diseases 0.000 title claims abstract description 15
- 238000006911 enzymatic reaction Methods 0.000 claims abstract description 43
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims abstract description 28
- 239000004005 microsphere Substances 0.000 claims abstract description 23
- 238000001514 detection method Methods 0.000 claims abstract description 20
- 239000004471 Glycine Substances 0.000 claims abstract description 14
- 150000005846 sugar alcohols Chemical class 0.000 claims abstract description 14
- 239000007853 buffer solution Substances 0.000 claims abstract description 13
- JUJWROOIHBZHMG-UHFFFAOYSA-O pyridinium Chemical compound C1=CC=[NH+]C=C1 JUJWROOIHBZHMG-UHFFFAOYSA-O 0.000 claims abstract description 13
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- 235000011130 ammonium sulphate Nutrition 0.000 claims description 6
- 229920000609 methyl cellulose Polymers 0.000 claims description 6
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- 238000007639 printing Methods 0.000 claims description 6
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- 239000002202 Polyethylene glycol Substances 0.000 claims description 4
- 239000004372 Polyvinyl alcohol Substances 0.000 claims description 4
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- 229930195729 fatty acid Natural products 0.000 claims description 4
- KTWOOEGAPBSYNW-UHFFFAOYSA-N ferrocene Chemical compound [Fe+2].C=1C=C[CH-]C=1.C=1C=C[CH-]C=1 KTWOOEGAPBSYNW-UHFFFAOYSA-N 0.000 claims description 4
- 235000019447 hydroxyethyl cellulose Nutrition 0.000 claims description 4
- 229920003063 hydroxymethyl cellulose Polymers 0.000 claims description 4
- 229940031574 hydroxymethyl cellulose Drugs 0.000 claims description 4
- 150000002596 lactones Chemical class 0.000 claims description 4
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- 229910052708 sodium Inorganic materials 0.000 claims description 4
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 claims description 4
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- LEHOTFFKMJEONL-UHFFFAOYSA-N Uric Acid Chemical compound N1C(=O)NC(=O)C2=C1NC(=O)N2 LEHOTFFKMJEONL-UHFFFAOYSA-N 0.000 abstract description 16
- TVWHNULVHGKJHS-UHFFFAOYSA-N Uric acid Natural products N1C(=O)NC(=O)C2NC(=O)NC21 TVWHNULVHGKJHS-UHFFFAOYSA-N 0.000 abstract description 16
- 229940116269 uric acid Drugs 0.000 abstract description 16
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 12
- 210000002700 urine Anatomy 0.000 abstract description 11
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- 125000002485 formyl group Chemical class [H]C(*)=O 0.000 abstract 1
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 15
- KDCGOANMDULRCW-UHFFFAOYSA-N 7H-purine Chemical compound N1=CNC2=NC=NC2=C1 KDCGOANMDULRCW-UHFFFAOYSA-N 0.000 description 6
- 235000010981 methylcellulose Nutrition 0.000 description 5
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- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- 201000005569 Gout Diseases 0.000 description 1
- 208000031226 Hyperlipidaemia Diseases 0.000 description 1
- 206010020772 Hypertension Diseases 0.000 description 1
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- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 1
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- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/62—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving uric acid
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/001—Enzyme electrodes
- C12Q1/004—Enzyme electrodes mediator-assisted
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/001—Enzyme electrodes
- C12Q1/005—Enzyme electrodes involving specific analytes or enzymes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/02—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance
- G01N27/04—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance by investigating resistance
- G01N27/06—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance by investigating resistance of a liquid
- G01N27/07—Construction of measuring vessels; Electrodes therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- General Health & Medical Sciences (AREA)
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- Molecular Biology (AREA)
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- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses a portable test strip for safely detecting hyperuricemia, and relates to the technical field of in-vitro diagnostic reagents. The test strip comprises an electrode and an enzyme reaction layer, wherein the enzyme reaction layer contains a biological reagent which reacts with a tested object, and the biological reagent comprises the following components in percentage by mass: 40-50% of buffer solution, 3-4% of high molecular compound, 2-3% of coenzyme, 5-12% of electron transfer mediator, 1-1.5% of defoamer, 10-15% of aldehyde pyridinium, 3-4% of glycine, 20-35% of microsphere and 5-6% of sugar alcohol. The test strip provided by the invention realizes rapid and accurate detection of uric acid content in urine by improving the enzyme reaction layer, is suitable for detection and use by individuals every day, and can provide convenience for families of patients and small clinics.
Description
Technical Field
The invention relates to the technical field of in-vitro diagnostic reagents, in particular to a portable test strip for safely detecting hyperuricemia.
Background
Uric acid is a metabolic product of the human body, 80% is derived from endogenous nuclear proteins, 20% is derived from exogenously ingested animals or foods containing purine, purine in foods is decomposed by the catalysis of enzymes, and purine is mainly derived from exogenesis in daily life, such as animal viscera and aquatic foods. Normally uric acid is mainly excreted by the kidneys. Currently, uric acid is mainly detected on a large-scale biochemical analyzer, but the method has the following problems: special devices are required, which are long and expensive, and can only be carried out in large hospitals. Clinical researches show that the increase of uric acid is directly related to patients with gout, hypertension, hyperlipidemia, diabetes and the like, so that the detection of the content of uric acid in urine has very important significance for clinical diagnosis. The clinical test results show that: the uric acid content in blood and the uric acid content in urine have certain correlation in general, and the crowd with higher uric acid at present has an ascending trend, so the problem of simply, quickly and low-cost detection of uric acid concentration is naturally proposed. At present, the main method for detecting uric acid is enzymatic detection, but the detection speed and the accuracy of a detection result are greatly influenced by an enzyme reaction layer, so that the deep research on the enzyme reaction layer has important significance for enhancing the use effect of the test strip.
Disclosure of Invention
In order to overcome the defects, the invention provides the portable and safe test strip for detecting hyperuricemia, and the test strip realizes the rapid and accurate detection of uric acid content in urine by improving the enzyme reaction layer, thereby being capable of providing convenience for families of patients and small clinics. The specific technical scheme is as follows:
a portable test strip for the safe detection of hyperuricemia comprising an electrode and an enzyme reaction layer containing a biological agent that reacts with a test subject, the biological agent comprising the following components in mass percent: 40-50% of buffer solution, 3-4% of high molecular compound, 2-3% of coenzyme, 5-12% of electron transfer mediator, 1-1.5% of defoamer, 10-15% of aldehyde pyridinium, 3-4% of glycine, 20-35% of microsphere and 5-6% of sugar alcohol.
Preferably, the buffer solution is one of a citric acid buffer solution, a phosphoric acid buffer solution or a tris buffer solution.
Preferably, the polymer compound is one of methylcellulose, sodium hydroxymethyl cellulose or hydroxyethyl cellulose.
Preferably, the coenzyme is nicotinamide adenine dinucleotide.
Preferably, the electron transfer mediator is one or a combination of more of potassium ferricyanide, ferrocene and derivatives thereof, complexes of nails or benzoquinone compounds.
Preferably, the preparation method of the enzyme reaction layer comprises the following steps: and sequentially adding the defoaming agent, the aldehyde pyridinium, the glycine and the microspheres into the buffer solution according to the mass ratio, stirring uniformly to obtain a first mixed solution, putting the first mixed solution into an electronic stirrer, adding the high molecular compound to obtain a solid preparation, and finally adding the coenzyme, the sugar alcohol and the electronic mediator into the solid preparation, and stirring uniformly to obtain the enzyme reaction layer.
Preferably, the test strip main body is an insulating substrate, a conductive layer, an electric insulating isolation layer and an enzyme reaction layer are arranged on the insulating substrate, and the conductive layer comprises a first working electrode, a second working electrode, a reference electrode and a short-circuit electrode; printing the conductive layer on the insulating substrate, printing the electric insulating isolation layer on the conductive layer, exposing part of the conductive layer at one end of the electric insulating isolation layer, arranging a rectangular reaction window at the other end of the electric insulating isolation layer, arranging the enzyme reaction layer in the reaction window, attaching a layer of bonding spacer on the electric insulating isolation layer, and finally attaching a layer of surface packaging sheet on the bonding spacer.
Preferably, the enzyme reaction layer may be applied in a coating manner to the rectangular reaction window and then dried by a constant temperature drying tunnel.
Preferably, the microsphere is one of polyvinyl alcohol microsphere, polyester lactone microsphere or chitosan microsphere, and can be used as a stabilizer.
Defoamers include liquid formulations, salts, proteins and plant extracts, which can be used as mediators to reduce the surface tension of the liquid, and preferably polyethylene glycol defoamers, fatty acid salt defoamers or ammonium sulfate can be selected.
Compared with the prior art, the invention has the beneficial effects that:
compared with the existing uric acid test strip, the uric acid test strip has the advantages of higher detection speed, more sensitive detection and more accurate result. The aldehyde pyridinium in the enzyme reaction layer has a quaternary ammonium salt structure and has positive charges, and is combined with coenzyme through electrostatic action to form a modified film, so that the aldehyde pyridinium can be used as a substrate medium for stabilizing enzyme slurry, the problem that the uniformity in a plate is poor and the deviation of a measurement result is large can be effectively prevented, and the microspheres are used as stabilizers and can be used for preventing the measurement deviation from being large due to aging rise, so that the accuracy of the detection result can be improved through the cooperation of the aldehyde pyridinium, the microspheres and the coenzyme. In addition, glycine in the enzyme reaction layer is used as a strong electrolyte and is matched with sugar alcohol and an electron transfer medium, so that the rapid conduction of electrons is realized, the reaction speed is further increased, and the detection time is shortened.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are required to be used in the description of the embodiments will be briefly described below.
FIG. 1 is a schematic view of the structure of a test strip of the present invention;
FIG. 2 is a graph of the response characteristics of test strip current in a preferred embodiment of the present invention.
Reference numerals:
1-conductive layer, 2-electrically insulating isolation layer, 3-enzyme reaction layer.
Detailed Description
The following detailed description of specific embodiments of the invention is, but it should be understood that the invention is not limited to specific embodiments.
Example 1
The portable test strip for safely detecting hyperuricemia comprises an electrode and an enzyme reaction layer, wherein the enzyme reaction layer contains a biological reagent reacting with a tested object, and the biological reagent comprises the following components in percentage by mass: 40% of citric acid buffer solution, 3% of methyl cellulose, 2% of nicotinamide adenine dinucleotide, 11% of potassium ferricyanide, 1% of polyethylene glycol defoamer, 10% of aldehyde pyridine salt, 3% of glycine, 25% of polyvinyl alcohol microsphere and 5% of sugar alcohol.
The preparation method of the enzyme reaction layer in the embodiment comprises the following steps: taking each component according to the mass ratio, sequentially adding a polyethylene glycol defoamer, aldehyde pyridinium, glycine and polyvinyl alcohol microspheres into a citric acid buffer solution, stirring uniformly to obtain a first mixed solution, putting the first mixed solution into an electronic stirrer, adding methylcellulose to obtain a solid preparation, and finally adding nicotinamide adenine dinucleotide, sugar alcohol and potassium ferricyanide into the solid preparation, and stirring uniformly to obtain an enzyme reaction layer.
Example 2
The portable test strip for safely detecting hyperuricemia comprises an electrode and an enzyme reaction layer, wherein the enzyme reaction layer contains a biological reagent reacting with a tested object, and the biological reagent comprises the following components in percentage by mass: 45.5% of phosphate buffer solution, 4% of sodium hydroxymethyl cellulose, 3% of nicotinamide adenine dinucleotide, 5% of ferrocene and derivatives thereof, 1.5% of fatty acid salt defoamer, 11% of aldehyde pyridine salt, 4% of glycine, 20% of polyester lactone microsphere and 6% of sugar alcohol.
The preparation method of the enzyme reaction layer in the embodiment comprises the following steps: taking each component according to the mass ratio, sequentially adding a fatty acid salt defoamer, aldehyde pyridinium, glycine and polyester lactone microspheres into a phosphoric acid buffer solution, stirring uniformly to obtain a first mixed solution, putting the first mixed solution into an electronic stirrer, then adding sodium hydroxymethyl cellulose to obtain a solid preparation, and finally adding nicotinamide adenine dinucleotide, sugar alcohol, ferrocene and derivatives thereof into the solid preparation, and stirring uniformly to obtain the enzyme reaction layer.
Example 3
The portable test strip for safely detecting hyperuricemia comprises an electrode and an enzyme reaction layer, wherein the enzyme reaction layer contains a biological reagent reacting with a tested object, and the biological reagent comprises the following components in percentage by mass: 50% of tris buffer, 3% of hydroxyethyl cellulose, 2% of nicotinamide adenine dinucleotide, 6% of a complex of nails, 1% of ammonium sulfate, 10% of aldehyde pyridinium, 3% of glycine, 20% of chitosan microspheres and 5% of sugar alcohol.
The preparation method of the enzyme reaction layer in the embodiment comprises the following steps: taking each component according to the mass ratio, sequentially adding ammonium sulfate, aldehyde pyridinium, glycine and chitosan microspheres into a tris buffer solution, stirring uniformly to obtain a first mixed solution, putting the first mixed solution into an electronic stirrer, adding hydroxyethyl cellulose to obtain a solid preparation, and finally adding a complex of nicotinamide adenine dinucleotide, sugar alcohol and nails into the solid preparation, and stirring uniformly to obtain an enzyme reaction layer.
Example 4
The portable test strip for safely detecting hyperuricemia comprises an electrode and an enzyme reaction layer, wherein the enzyme reaction layer contains a biological reagent reacting with a tested object, and the biological reagent comprises the following components in percentage by mass: 45% of citric acid buffer solution, 3% of methyl cellulose, 2% of nicotinamide adenine dinucleotide, 5% of benzoquinone compound, 1% of ammonium sulfate, 10% of aldehyde pyridine salt, 3% of glycine, 25% of chitosan microsphere and 6% of sugar alcohol.
The preparation method of the enzyme reaction layer in the embodiment comprises the following steps: taking each component according to the mass ratio, sequentially adding ammonium sulfate, aldehyde pyridinium, glycine and chitosan microspheres into a citric acid buffer solution, stirring uniformly to obtain a first mixed solution, putting the first mixed solution into an electronic stirrer, adding methyl cellulose to obtain a solid preparation, and finally adding nicotinamide adenine dinucleotide, sugar alcohol and benzoquinone compounds into the solid preparation, and stirring uniformly to obtain an enzyme reaction layer.
Example 5
The uric acid test strip main body of the embodiment is an insulating substrate, the insulating substrate is a PC sheet with the thickness of 0.35mm, and in another embodiment also can be a PET sheet, a conductive layer 1, an electric insulating isolation layer 2 and an enzyme reaction layer 3 are arranged on the insulating substrate, and the conductive layer 1 comprises a first working electrode, a second working electrode, a reference electrode and a short-circuit electrode; firstly, printing a conductive layer 1 on an insulating substrate in a screen printing mode, then printing an electric insulating isolation layer 2 on the conductive layer 1, exposing part of the conductive layer 1 at one end of the electric insulating isolation layer 2, arranging a rectangular reaction window at the other end, arranging an enzyme reaction layer 3 in the reaction window, then attaching a layer of bonding spacer on the electric insulating isolation layer 2, arranging a rectangular siphon sample guiding groove at the corresponding position of the spacer and the reaction window, arranging an air vent at one side close to the electrode conducting part, arranging a sampling port at one end far away from the electrode conducting part, mutually penetrating and communicating the sampling port, the siphon sample guiding groove and the air vent, and finally attaching a layer of surface packaging sheet on the bonding spacer. Wherein the enzyme reaction layer 3 may be applied in a coating manner to a rectangular reaction window and then dried by a constant temperature drying tunnel.
In this embodiment, the conductive layer 1 is made of a conductive paste material suitable for screen printing, and carbon ink, gold ink, silver ink, carbon-silver mixed ink, volatile graphite or copper ink may be selected.
In this embodiment, the insulating tape made of a thin layer-PET material and having an electrical insulating property is used as the insulating layer 2, and it is noted that an insulating tape made of PVC, an ultraviolet light-drying type insulating paint, and a heat-drying type insulating paint may be used, and the thickness of the insulating layer may be 0.1mm, or may be 0.2mm in another embodiment.
In this example, the enzyme reaction layer prepared in example 4 was used, and in another example, the enzyme reaction layer prepared in example 1, example 2 or example 3 was used.
The test method of the uric acid test strip comprises the following steps: the measuring instrument adopts the biological sensing technology, the enzyme immobilization technology and the bioelectrochemical microcurrent detection technology, the electrode conduction part of the test strip is inserted into the measuring instrument, a reference electrode and a short circuit electrode form a passage through an ejector pin in the instrument, the measuring instrument is connected and started automatically, the measuring instrument applies direct current voltage between a working electrode and the reference electrode, microcurrent signals generated by urine and enzyme reactants are tested, and then the working electrode and the reference electrode are closed. Then high-frequency alternating voltage is applied between the first working electrode and the second working electrode, the resistance value between the first working electrode and the second working electrode is tested, and the concentration value of the urine acid in the urine sample is calculated through the microcomputer chip.
To illustrate the effectiveness of the test strips of the present invention, applicants compared the precision of the test strips of the present invention (hereinafter UUA 02) to the commercially available test strips (Ai Ke, zhongsheng ZEALSON). The results were as follows:
TABLE 1 comparison of urine sample (No. 1) detection results
TABLE 2 comparison of urine sample (No. 2) detection results
TABLE 3 comparison of urine sample (No. 3) detection results
TABLE 4 comparison of urine sample (No. 4) detection results
In summary, the test strip of the invention has shorter detection time and higher accuracy. While figure 2 shows that the current value tends to be smooth after 5 seconds, so that the test strip of the present invention can achieve a rapid detection of 5 seconds.
The foregoing descriptions of specific exemplary embodiments of the present invention are presented for purposes of illustration and description. It is not intended to limit the invention to the precise form disclosed, and obviously many modifications and variations are possible in light of the above teaching. The exemplary embodiments were chosen and described in order to explain the specific principles of the invention and its practical application to thereby enable one skilled in the art to make and utilize the invention in various exemplary embodiments and with various modifications as are suited to the particular use contemplated. It is intended that the scope of the invention be defined by the claims and their equivalents.
Claims (10)
1. A portable, safety-testing strip for hyperuricemia comprising an electrode and an enzyme reaction layer, the enzyme reaction layer comprising a biological agent that reacts with a test subject, wherein the biological agent comprises, in mass percent: 40-50% of buffer solution, 3-4% of high molecular compound, 2-3% of coenzyme, 5-12% of electron transfer mediator, 1-1.5% of defoamer, 10-15% of aldehyde pyridinium, 3-4% of glycine, 20-35% of microsphere and 5-6% of sugar alcohol.
2. The portable safety-testing strip of claim 1, wherein the buffer is one of a citrate buffer, a phosphate buffer, or a tris buffer.
3. The portable safety test strip of claim 1, wherein the polymer compound is one of methylcellulose, sodium hydroxymethylcellulose, or hydroxyethylcellulose.
4. The portable, safe test strip for hyperuricemia according to claim 1, wherein the coenzyme is nicotinamide adenine dinucleotide.
5. The portable, safe-detecting hyperuricemia test strip of claim 1, wherein the electron transfer mediator is one or more of potassium ferricyanide, ferrocene and its derivatives, a pinned complex, or benzoquinone compounds.
6. The portable, safe test strip for detecting hyperuricemia according to claim 1, wherein the antifoaming agent is one of a polyethylene glycol antifoaming agent, a fatty acid salt antifoaming agent, or ammonium sulfate.
7. The portable safety test strip of claim 1, wherein the microsphere is one of a polyvinyl alcohol microsphere, a polyester lactone microsphere, or a chitosan microsphere.
8. The portable safety-testing strip of any one of claims 1 to 7, wherein the enzyme reaction layer is prepared by the steps of: and sequentially adding the defoaming agent, the aldehyde pyridinium, the glycine and the microspheres into the buffer solution according to the mass ratio, stirring uniformly to obtain a first mixed solution, putting the first mixed solution into an electronic stirrer, adding the high molecular compound to obtain a solid preparation, and finally adding the coenzyme, the sugar alcohol and the electronic mediator into the solid preparation, and stirring uniformly to obtain the enzyme reaction layer.
9. The portable, safe-detection hyperuricemia-testing strip of claim 1, wherein the testing strip body is an insulating substrate with a conductive layer, an electrically insulating isolation layer and an enzyme reaction layer thereon, the conductive layer comprising a first working electrode, a second working electrode, a reference electrode and a shorting electrode; printing the conductive layer on the insulating substrate, printing the electric insulating isolation layer on the conductive layer, exposing part of the conductive layer at one end of the electric insulating isolation layer, arranging a rectangular reaction window at the other end of the electric insulating isolation layer, arranging the enzyme reaction layer in the reaction window, attaching a layer of bonding spacer on the electric insulating isolation layer, and finally attaching a layer of surface packaging sheet on the bonding spacer.
10. The portable, safety-testing strip of claim 9, wherein the enzyme reaction layer is applied in a coating fashion to the rectangular reaction window and then dried in a constant temperature drying tunnel.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311567293.7A CN117604070A (en) | 2023-11-23 | 2023-11-23 | Portable test strip for safely detecting hyperuricemia |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311567293.7A CN117604070A (en) | 2023-11-23 | 2023-11-23 | Portable test strip for safely detecting hyperuricemia |
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| Publication Number | Publication Date |
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| CN117604070A true CN117604070A (en) | 2024-02-27 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202311567293.7A Pending CN117604070A (en) | 2023-11-23 | 2023-11-23 | Portable test strip for safely detecting hyperuricemia |
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| Country | Link |
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| CN (1) | CN117604070A (en) |
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2023
- 2023-11-23 CN CN202311567293.7A patent/CN117604070A/en active Pending
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