CN117586875B - Biological enzyme catalytic efficiency testing arrangement - Google Patents
Biological enzyme catalytic efficiency testing arrangement Download PDFInfo
- Publication number
- CN117586875B CN117586875B CN202410071319.7A CN202410071319A CN117586875B CN 117586875 B CN117586875 B CN 117586875B CN 202410071319 A CN202410071319 A CN 202410071319A CN 117586875 B CN117586875 B CN 117586875B
- Authority
- CN
- China
- Prior art keywords
- gear
- cylinder
- damping
- outer cylinder
- intermediate shaft
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 102000004190 Enzymes Human genes 0.000 title claims abstract description 28
- 108090000790 Enzymes Proteins 0.000 title claims abstract description 28
- 238000012360 testing method Methods 0.000 title claims abstract description 27
- 230000003197 catalytic effect Effects 0.000 title claims abstract description 24
- 238000006243 chemical reaction Methods 0.000 claims abstract description 43
- 238000006555 catalytic reaction Methods 0.000 claims abstract description 23
- 238000013016 damping Methods 0.000 claims description 65
- 239000003921 oil Substances 0.000 claims description 23
- 238000007789 sealing Methods 0.000 claims description 14
- 230000002093 peripheral effect Effects 0.000 claims description 12
- 239000003054 catalyst Substances 0.000 claims description 10
- 239000000758 substrate Substances 0.000 claims description 10
- 239000010720 hydraulic oil Substances 0.000 claims description 3
- 230000000007 visual effect Effects 0.000 abstract description 4
- 230000000670 limiting effect Effects 0.000 description 9
- 239000003973 paint Substances 0.000 description 9
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 8
- 239000003550 marker Substances 0.000 description 6
- 239000007789 gas Substances 0.000 description 4
- 238000004891 communication Methods 0.000 description 3
- 230000008878 coupling Effects 0.000 description 3
- 238000010168 coupling process Methods 0.000 description 3
- 238000005859 coupling reaction Methods 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 102000016938 Catalase Human genes 0.000 description 2
- 108010053835 Catalase Proteins 0.000 description 2
- 230000001070 adhesive effect Effects 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 239000012459 cleaning agent Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000000376 reactant Substances 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 1
- 235000017491 Bambusa tulda Nutrition 0.000 description 1
- 241001330002 Bambuseae Species 0.000 description 1
- 241000870659 Crassula perfoliata var. minor Species 0.000 description 1
- 235000015334 Phyllostachys viridis Nutrition 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 239000011425 bamboo Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000011942 biocatalyst Substances 0.000 description 1
- 238000005842 biochemical reaction Methods 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000010422 painting Methods 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/18—Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Cell Biology (AREA)
- Analytical Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to the technical field of enzymology devices, in particular to a biological enzyme catalysis efficiency testing device which comprises a reaction container, an inner cylinder, an outer cylinder and a line drawing pen, wherein the top of the inner cylinder is sealed and communicated with the reaction container, a piston is connected in the inner cylinder in a sliding manner, the outer cylinder and the inner cylinder are coaxially sleeved, the outer cylinder and the inner cylinder can rotate relatively, and the outer cylinder can rotate around the axis of the outer cylinder. According to the utility model, the speed of the catalytic reaction can be converted and displayed into the screw pitch of the spiral line drawn by the line drawing pen on the wall of the outer barrel by the arrangement of the inner barrel, the outer barrel and the line drawing pen, at the moment, a worker can judge the catalytic reaction efficiency according to the screw pitch of the spiral line, the larger the screw pitch of the spiral line is, the higher the catalytic reaction efficiency is, the smaller the screw pitch of the spiral line is, the lower the catalytic reaction efficiency is, compared with the prior art, the operation step for testing the biological enzyme catalytic efficiency is simpler, the display result of the catalytic reaction efficiency is more visual, and the use convenience is higher.
Description
Technical Field
The utility model relates to the technical field of enzymology devices, in particular to a biological enzyme catalysis efficiency testing device.
Background
Biological enzymes, which are biocatalysts that accelerate the reaction rate of biochemical reactions, can be extracted from living beings, for example, catalase can be extracted from fresh bamboo. In experiments, it was found that the rate of gas generation by the reaction of the fresh column-soaked water with the addition of hydrogen peroxide and reactants into the tube was significantly greater than the rate of hydrogen peroxide and reactants without the addition of fresh column-soaked water.
Since the catalytic efficiency of chemical reactions by different biological enzymes varies, in order to obtain the optimal biological enzymes for the chemical reactions, it is necessary to test the chemical reaction efficiency after adding various biological enzymes. For example, chinese patent publication No. CN201047837Y discloses a gas catalytic efficiency detecting device capable of detecting the reaction efficiency of a gas catalytic reaction, but the following drawbacks still exist: the detection process is complex in operation, the display result of the reaction efficiency is not visual, and the use convenience is low.
Disclosure of Invention
Accordingly, it is necessary to provide a device for testing the catalytic efficiency of biological enzymes, which aims at the problems of the conventional device for testing the catalytic efficiency.
The above purpose is achieved by the following technical scheme:
a biological enzyme catalytic efficiency testing device comprises:
a reaction vessel;
the top of the inner cylinder is sealed and communicated with the reaction vessel, and a piston is connected in the inner cylinder in a sliding way;
the outer cylinder and the inner cylinder are coaxially sleeved, the outer cylinder and the inner cylinder can rotate relatively, and the outer cylinder can rotate around the axis of the outer cylinder;
the nib of drawing pencil rotates the contact with the periphery wall of urceolus, and the body and the piston coupling of drawing pencil to make the piston can drive drawing pencil synchronous movement.
In one embodiment, the device for testing the catalytic efficiency of the biological enzyme further comprises a sticker, one surface of the sticker can be used for writing, the other surface of the sticker is sticky, and the sticky surface of the sticker is sticky to the peripheral wall of the outer cylinder.
In one embodiment, the device for testing the catalytic efficiency of the biological enzyme further comprises a housing, wherein a power assembly is arranged in the housing and used for driving the outer cylinder to rotate around the axis of the outer cylinder.
In one embodiment, the power assembly comprises a power source, a first belt wheel, a second belt wheel and an intermediate shaft, wherein the power source is arranged on the shell, the output end of the power source is fixedly connected with the first belt wheel, the intermediate shaft is arranged on the shell, the second belt wheel is arranged on the intermediate shaft, the first belt wheel is connected with the second belt wheel through a belt, a first gear is further arranged on the periphery of the intermediate shaft, the first gear and the second belt wheel can synchronously rotate, a second gear is fixedly connected with the periphery of the outer barrel, and the second gear is meshed with the first gear.
In one embodiment, a third gear is arranged on the periphery of the intermediate shaft and above the first gear, the diameter of the third gear is larger than that of the first gear, a fourth gear is fixedly connected to the periphery of the outer cylinder, and the diameter of the fourth gear is smaller than that of the second gear;
the intermediate shaft is also capable of moving a predetermined distance along its axis such that the third gear meshes with the fourth gear and the first gear is disengaged from the second gear.
In one embodiment, a U-shaped frame is fixedly connected between the pen body and the piston of the line drawing pen.
In one embodiment, the hollow two ends of the middle shaft are opened, the upper part of the middle shaft is sleeved with a damping cylinder, the damping cylinder and the middle shaft can synchronously move and relatively rotate, the inner peripheral wall of the shell is provided with a connecting frame, and the connecting frame is in friction contact with the outer peripheral wall of the damping cylinder;
the damping cylinder is slidably connected with a damping oil plug, the damping oil plug divides the interior of the damping cylinder into a first area and a second area, hydraulic oil is filled in the first area and the second area, a damping small hole is formed in the damping oil plug and communicated with the first area and the second area, the lower end of the damping oil plug is connected with a damping rod, one end of the damping rod, which is far away from the damping oil plug, is fixedly connected with an elastic buckle, and one end of the U-shaped frame, which is far away from the piston, is fixedly connected with a clamping plate;
when the elastic buckle is positioned in the area of the middle shaft, the elastic buckle and the clamping plate are mutually clamped, so that the elastic buckle can be driven to synchronously move downwards when the clamping plate moves downwards; when the elastic buckle is positioned in the area which is not in the intermediate shaft, the elastic buckle and the clamping plate are separated from each other to be clamped, and when the clamping plate moves downwards, the elastic buckle is not driven to move synchronously.
In one embodiment, the shell is provided with a limit groove, the limit groove extends along the axis direction of the outer barrel, and the pen body of the line drawing pen is slidably connected in the limit groove.
In one embodiment, a viewing area is provided on one circumferential side of the housing.
In one embodiment, a sealing plug is arranged at the top of the reaction vessel, and a substrate feeding pipe and a catalyst feeding pipe are inserted into the sealing plug.
The beneficial effects of the utility model are as follows:
according to the utility model, the speed of the catalytic reaction can be converted and displayed into the screw pitch of the spiral line drawn by the line drawing pen on the wall of the outer barrel by the arrangement of the inner barrel, the outer barrel and the line drawing pen, at the moment, a worker can judge the catalytic reaction efficiency according to the screw pitch of the spiral line, the larger the screw pitch of the spiral line is, the higher the catalytic reaction efficiency is, the smaller the screw pitch of the spiral line is, the lower the catalytic reaction efficiency is, compared with the prior art, the operation step for testing the biological enzyme catalytic efficiency is simpler, the display result of the catalytic reaction efficiency is more visual, and the use convenience is higher.
Drawings
FIG. 1 is a schematic diagram showing the whole device for testing the catalytic efficiency of biological enzyme according to the present utility model;
FIG. 2 is a front view of a device for testing the catalytic efficiency of a biological enzyme according to the present utility model;
FIG. 3 is a cross-sectional view A-A of FIG. 2;
FIG. 4 is a top view of a biological enzyme catalytic efficiency testing device of the present utility model;
FIG. 5 is a cross-sectional view B-B of FIG. 4;
FIG. 6 is a cross-sectional view of C-C of FIG. 4;
fig. 7 is an enlarged view of the structure at D in fig. 6.
Wherein:
100. a reaction vessel; 110. a sealing plug; 111. a substrate feed tube; 112. a catalyst feed tube; 120. a communicating pipe; 210. an inner cylinder; 211. a piston; 220. an outer cylinder; 300. a line drawing pen; 400. a housing; 410. a connecting frame; 420. a limit groove; 430. an observation area; 440. a base; 500. a power assembly; 510. a power source; 520. a first pulley; 530. a second pulley; 540. an intermediate shaft; 550. a first gear; 560. a second gear; 570. a third gear; 580. a fourth gear; 600. a U-shaped frame; 610. a clamping plate; 700. a damping cylinder; 710. damping oil plugs; 711. damping pinholes; 720. a damping rod; 730. and an elastic buckle.
Detailed Description
The present utility model will be further described in detail below with reference to examples, which are provided to illustrate the objects, technical solutions and advantages of the present utility model. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the utility model.
The numbering of components herein, such as "first," "second," etc., is used merely to distinguish between the described objects and does not have any sequential or technical meaning. The terms "coupled" and "connected," as used herein, are intended to encompass both direct and indirect coupling (coupling), unless otherwise indicated. In the description of the present utility model, it should be understood that the terms "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are merely for convenience in describing the present utility model and simplifying the description, and do not indicate or imply that the device or element in question must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present utility model.
In the present utility model, unless expressly stated or limited otherwise, a first feature "up" or "down" a second feature may be the first and second features in direct contact, or the first and second features in indirect contact via an intervening medium. Moreover, a first feature being "above," "over" and "on" a second feature may be a first feature being directly above or obliquely above the second feature, or simply indicating that the first feature is level higher than the second feature. The first feature being "under", "below" and "beneath" the second feature may be the first feature being directly under or obliquely below the second feature, or simply indicating that the first feature is less level than the second feature.
As shown in fig. 1-7, a device for testing the catalytic efficiency of biological enzymes comprises a reaction vessel 100, an inner cylinder 210, an outer cylinder 220 and a marker 300, wherein the top of the inner cylinder 210 is sealed and is communicated with the reaction vessel 100, a piston 211 is slidably connected in the inner cylinder 210, the outer cylinder 220 is coaxially sleeved with the inner cylinder 210, the outer cylinder 220 can rotate relative to the inner cylinder 210, the outer cylinder 220 can rotate around the axis thereof, the pen point of the marker 300 is in rotary contact with the peripheral wall of the outer cylinder 220, the marker 300 is any one of a pencil or a marker, and the pencil body of the marker 300 is connected with the piston 211, so that the piston 211 can drive the marker 300 to synchronously move.
To be added, in order to add a reaction substrate and a bio-enzyme catalyst into the reaction vessel 100, specifically, as shown in fig. 1, a sealing stopper 110 is provided at the top of the reaction vessel 100, the sealing stopper 110 is used to seal the top of the reaction vessel 100, a substrate feed pipe 111 and a catalyst feed pipe 112 are inserted into the reaction vessel 100, a substrate is added into the reaction vessel 100 through the substrate feed pipe 111, and a bio-enzyme catalyst is added into the reaction vessel 100 through the catalyst feed pipe 112.
It is also to be added that, in order to achieve the top sealing of the inner tube 210 and to allow the inner tube 210 to communicate with the reaction vessel 100, specifically, as shown in fig. 5, a sealing rubber stopper is provided at the top of the inner tube 210, a communication tube 120 is inserted onto the sealing rubber stopper, and one end of the communication tube 120 remote from the sealing rubber stopper is inserted onto the sealing plug 110, so that the inner tube 210 communicates with the reaction vessel 100 through the communication tube 120. In other embodiments, a detachable plug is further disposed on the sealing rubber plug, when the piston 211 needs to be reset, the plug is removed, at this time, no pressure difference exists between the upper and lower parts of the piston 211, and at this time, the drawing pen 300 is pulled, so that the piston 211 can be driven to move up and down along the inner wall of the inner cylinder 210; when the air tightness of the device of the inner cylinder 210 is checked, the stopper is plugged onto the sealing rubber stopper, and when the drawing pen 300 is pulled downward at this time, a significant resistance is felt, so that the air tightness of the inner cylinder 210 is good.
When the device is used, the outer cylinder 220 rotates at a constant speed around the axis of the outer cylinder 220, then a substrate (such as hydrogen peroxide) required by the reaction is added into the reaction container 100, then a biological enzyme catalyst (such as catalase) required by the substrate reaction is added into the reaction container 100, under the catalysis of the biological enzyme catalyst, the hydrogen peroxide is decomposed into water and oxygen, and the generated oxygen enters the upper area of the inner cylinder 210 through the communicating pipe 120, so that the upper air pressure of the inner cylinder 210 is increased, the piston 211 is pushed by the air pressure to move downwards along the inner wall of the inner cylinder 210, the drawing pen 300 is driven by the piston 211 to move downwards synchronously, so that the drawing pen 300 draws a spiral line on the wall of the outer cylinder 220, at the moment, a worker can judge the efficiency of the catalytic reaction according to the pitch of the spiral line, the larger the pitch of the spiral line is higher, the higher the catalytic reaction efficiency is illustrated, the smaller pitch of the spiral line is illustrated, the reaction efficiency of the catalytic reaction is illustrated lower, compared with the prior art, the operation steps for testing the biological enzyme catalytic efficiency are simpler, the display result of the catalytic reaction efficiency is more visual, and the convenience is higher.
It is also added that in other embodiments, the paint gun may be used in place of the paint brush 300 such that the spray orifice of the paint gun is perpendicular to the axis of the outer barrel 220 and such that the spray orifice of the paint gun is a predetermined distance from the outer peripheral wall of the outer barrel 220, at which time the quick-drying paint is sprayed onto the surface of the outer barrel 220 by the paint gun, and a clear spiral line may also be formed on the surface of the outer barrel 220. After the paint cleaning agent is used, paint on the surface of the outer barrel 220 can be cleaned only by smearing the paint cleaning agent on the surface of the outer barrel 220.
It will be appreciated that the piston 211 should be selected to be lightweight, so as to balance the frictional resistance of the piston 211 when moving by the weight of the piston 211, so that the movement of the piston 211 is more sensitive, and interference of other external forces to the movement of the piston 211 is avoided.
In a further embodiment, the bio-enzyme catalytic efficiency testing device further comprises a sticker, one side of the sticker is used for writing, the other side of the sticker has an adhesive property, and the adhesive side of the sticker is adhered to the outer peripheral wall of the outer cylinder 220.
The setting is in order to prevent the surface of urceolus 220 and painting pen 300 or paint direct contact, and cause the urceolus 220 surface to appear damaging, not only can avoid urceolus 220 surface to appear damaging through pasting the sticker on urceolus 220 surface, and the staff also can tear down the sticker after the test from urceolus 220 moreover, makes things convenient for the pitch of helix on the accurate measurement sticker of staff to obtain accurate numerical value.
In a further embodiment, as shown in fig. 5, a U-shaped frame 600 is fixedly connected between the body of the line drawing pen 300 and the piston 211, and the body of the line drawing pen 300 and the piston 211 can be fixedly connected together by providing the U-shaped frame 600, so that the line drawing pen 300 can move synchronously with the piston 211.
In a further embodiment, as shown in fig. 6 and 7, the bio-enzyme catalytic efficiency testing apparatus further includes a housing 400, a power assembly 500 is disposed in the housing 400, the power assembly 500 is used for driving the outer cylinder 220 to rotate around an axis thereof, the power assembly 500 includes a power source 510, a first gear 520, a second gear 530 and an intermediate shaft 540, the power source 510 is disposed on the housing 400, an output end of the power source 510 is fixedly connected with the first gear 520, the intermediate shaft 540 is disposed on the housing 400, the second gear 530 is rotatably disposed on the intermediate shaft 540, the first gear 520 is in belt connection with the second gear 530, a first gear 550 is further disposed on an outer circumference of the intermediate shaft 540, the first gear 550 and the second gear 530 can synchronously rotate, a second gear 560 is fixedly connected on an outer circumference of the outer cylinder 220, and the second gear 560 is meshed with the first gear 550.
When the outer cylinder 220 needs to be driven to rotate around the axis thereof, the power source 510 is started, so that the output shaft of the power source 510 drives the first belt pulley 520 to rotate, the first belt pulley 520 drives the second belt pulley 530 to rotate through belt transmission, the second belt pulley 530 drives the intermediate shaft 540 to rotate, the intermediate shaft 540 drives the first gear 550 to rotate, the first gear 550 drives the second gear 560 to rotate, and the second gear 560 drives the outer cylinder 220 to rotate around the axis thereof, thereby enabling the outer cylinder 220 to rotate around the axis thereof.
In a further embodiment, in order to avoid that the pitch of the spiral line cannot be displayed completely on the circumferential wall of the outer cylinder 220 when the reaction rate is too fast, the pitch of the spiral line can be shortened by increasing the rotation speed of the outer cylinder 220 for the catalytic reaction with the fast reaction rate, so that the pitch of the spiral line can be displayed completely on the outer cylinder 220, specifically, a third gear 570 is disposed on the outer circumference of the intermediate shaft 540 above the first gear 550, the diameter of the third gear 570 is larger than the diameter of the first gear 550, a fourth gear 580 is fixedly connected to the outer circumference of the outer cylinder 220, the diameter of the fourth gear 580 is smaller than the diameter of the second gear 560, and the intermediate shaft 540 can be moved along its axis by a preset distance so that the third gear 570 is meshed with the fourth gear 580, and the first gear 550 is out of mesh with the second gear 560.
When the reaction rate between the substrate in the reaction vessel 100 and the bio-enzyme catalyst is fast, the worker can observe that the speed of generating bubbles in the reaction vessel 100 is significantly increased, and at this time, the worker can move the intermediate shaft 540 downward along the axis thereof by a preset distance to engage the third gear 570 with the fourth gear 580, the first gear 550 is disengaged from the second gear 560, and since the diameter of the third gear 570 is larger than that of the first gear 550 and the diameter of the second gear 560 is larger than that of the fourth gear 580, the rotational speed of the outer cylinder 220 is increased when the third gear 570 is engaged with the fourth gear 580, thereby enabling the pitch of the spiral line to be displayed completely on the outer cylinder 220, which is convenient for the worker to record.
It is also added that, in order to drive the intermediate shaft 540 to move downward along the axis thereof by a predetermined distance, specifically, an electric telescopic rod may be disposed in the housing 400, the axis of the electric telescopic rod is parallel to the axis of the outer cylinder 220, and a shaft sleeve is fixedly connected to the telescopic end of the electric telescopic end, so that the outer cylinder 220 is rotatably disposed in the shaft sleeve, and the shaft sleeve is driven to move by the movement of the telescopic end of the electric telescopic rod, so that the outer cylinder 220 is driven to move along the axis thereof by the shaft sleeve by a predetermined distance.
In a further embodiment, as shown in fig. 7, the two hollow ends of the interior of the intermediate shaft 540 are opened, the damping cylinder 700 is sleeved on the upper portion of the intermediate shaft 540, the damping cylinder 700 and the intermediate shaft 540 can synchronously move and relatively rotate, a connecting frame 410 is arranged on the inner peripheral wall of the housing 400, the connecting frame 410 is in friction contact with the outer peripheral wall of the damping cylinder 700, a damping oil plug 710 is slidably connected in the damping cylinder 700, the damping oil plug 710 divides the interior of the damping cylinder 700 into a first area and a second area, hydraulic oil is filled in the first area and the second area, a damping small hole 711 is formed in the damping oil plug 710, the damping small hole 711 is communicated with the first area and the second area, a damping rod 720 is connected to the lower end of the damping oil plug 710, one end of the damping rod 720, which is far away from the damping oil plug 710, is fixedly connected with an elastic buckle 730, one end of the U-shaped frame 600, which is far away from the piston 211, is fixedly connected with a clamping plate 610, when the elastic buckle 730 is located in the area of the intermediate shaft 540, the elastic buckle 730 is clamped with the clamping plate 610, so that the elastic buckle 730 can be driven to synchronously move downwards when the clamping plate 610 moves downwards; when the elastic buckle 730 is located in the non-intermediate shaft 540, the elastic buckle 730 is separated from the clamping plate 610, and the clamping plate 610 moves downward, so that the elastic buckle 730 is not driven to move synchronously.
When the reaction rate in the reaction vessel 100 is slow, the rate of gas generation is slow, the moving speed of the piston 211 is slow, so that the damping oil plug 710 slowly moves in the damping cylinder 700, at the moment, the damping effect exerted by the damping small holes 711 is small, the friction force between the damping oil plug 710 and the damping cylinder 700 is smaller than the friction force between the connecting frame 410 and the cylinder wall of the damping cylinder 700, when the reaction rate in the reaction vessel 100 is slow, the U-shaped frame 600 moves downwards to drive the clamping plate 610 to move downwards, the clamping plate 610 drives the damping rod 720 to move downwards through the elastic buckles 730 mutually clamped with the clamping plate 610, the damping rod 720 pulls the damping oil plug 710 to move downwards along the inner wall of the damping cylinder 700, and when the elastic buckles 730 move downwards to move out of the middle shaft 540, the elastic buckles 730 lose the limiting effect of the inner wall of the middle shaft 540, so that the elastic buckles 730 are separated from the clamping plate 610, and the U-shaped frame 600 does not drive the elastic buckles 730 to move downwards synchronously through the clamping plate 610; when the catalytic reaction rate in the reaction vessel 100 is fast, the moving speed of the U-shaped frame 600 is fast, the U-shaped frame 600 drives the damping rod 720 to move downwards through the clamping plate 610 and the elastic buckle 730, the damping rod 720 drives the damping oil plug 710 to move downwards, the damping effect exerted by the damping small holes 711 is remarkable because the moving speed of the damping oil plug 710 is fast at the moment, the friction force between the damping oil plug 710 and the damping cylinder 700 is larger than the friction force between the connecting frame 410 and the damping cylinder 700, the damping oil plug 710 drives the damping cylinder 700 to move downwards for a preset distance, so that the first gear 550 is disengaged from the third gear 570, the third gear 570 is engaged with the fourth gear 580, and the rotating speed of the outer cylinder 220 is increased at the moment, so that the spiral line can be displayed on the outer cylinder 220 completely.
It should be further noted that, the outer cylinder 220 in the present utility model rotates at a low speed at the initial stage of the catalytic reaction, so as to reduce the number of circles drawn at the initial stage, avoid the influence of deepening scratches on the observation or the scratch of the sticker by the line drawing pen 300, and adapt to some catalytic reactions with slow reaction rate due to the low speed at the initial stage, so as to avoid the difficulty in observation due to too small pitch.
In a further embodiment, as shown in fig. 1, a limiting groove 420 is formed on the housing 400, the limiting groove 420 extends along the axial direction of the outer barrel 220, and the body of the line drawing pen 300 is slidably connected in the limiting groove 420.
The limiting groove 420 is configured to guide movement of the line drawing pen 300, so that the line drawing pen 300 moves downward along a straight line under the limiting effect of the limiting groove 420, and a spiral line track drawn by the line drawing pen 300 is prevented from being deviated.
In a further embodiment, as shown in fig. 1, a viewing area 430 is provided on one side of the housing 400 in the circumferential direction, the viewing area 430 being provided to facilitate the measurement of the helical pitch on the outer barrel 220 by a worker.
In a further embodiment, as shown in fig. 5, a base 440 is provided at the bottom of the housing 400, and the base 440 is used to support the housing 400, so that the housing 400 can be kept stable when the bio-enzyme catalytic efficiency testing device is used.
The technical features of the above embodiments may be arbitrarily combined, and all possible combinations of the technical features in the above embodiments are not described for brevity of description, however, as long as there is no contradiction between the combinations of the technical features, they should be considered as the scope of the description.
The above examples merely represent a few embodiments of the present utility model, which are described in more detail and are not to be construed as limiting the scope of the present utility model. It should be noted that it will be apparent to those skilled in the art that several variations and modifications can be made without departing from the spirit of the utility model, which are all within the scope of the utility model. Accordingly, the scope of the utility model should be assessed as that of the appended claims.
Claims (5)
1. A biological enzyme catalytic efficiency testing device, comprising:
a reaction vessel;
the top of the inner cylinder is sealed and communicated with the reaction vessel, and a piston is connected in the inner cylinder in a sliding way;
the outer cylinder and the inner cylinder are coaxially sleeved, the outer cylinder and the inner cylinder can rotate relatively, and the outer cylinder can rotate around the axis of the outer cylinder;
the pen point of the line drawing pen is in rotary contact with the outer peripheral wall of the outer cylinder, and the pen body of the line drawing pen is connected with the piston, so that the piston can drive the line drawing pen to synchronously move; the biological enzyme catalysis efficiency testing device further comprises a shell, wherein a power assembly is arranged in the shell and used for driving the outer barrel to rotate around the axis of the outer barrel; the power assembly comprises a power source, a first belt pulley, a second belt pulley and an intermediate shaft, wherein the power source is arranged on the shell, the output end of the power source is fixedly connected with the first belt pulley, the intermediate shaft is arranged on the shell, the second belt pulley is arranged on the intermediate shaft, the first belt pulley is connected with the second belt pulley through a belt, a first gear is further arranged on the periphery of the intermediate shaft, the first gear and the second belt pulley can synchronously rotate, a second gear is fixedly connected on the periphery of the outer cylinder, and the second gear is meshed with the first gear; a third gear is arranged on the periphery of the intermediate shaft and above the first gear, the diameter of the third gear is larger than that of the first gear, a fourth gear is fixedly connected to the periphery of the outer cylinder, and the diameter of the fourth gear is smaller than that of the second gear;
the intermediate shaft can also move along the axis of the intermediate shaft by a preset distance so that the third gear is meshed with the fourth gear, and the first gear is disengaged from the second gear; a U-shaped frame is fixedly connected between the pen body and the piston of the line drawing pen; the damping cylinder and the intermediate shaft can synchronously move and relatively rotate, a connecting frame is arranged on the inner peripheral wall of the shell, and the connecting frame is in friction contact with the outer peripheral wall of the damping cylinder;
the damping cylinder is slidably connected with a damping oil plug, the damping oil plug divides the interior of the damping cylinder into a first area and a second area, hydraulic oil is filled in the first area and the second area, a damping small hole is formed in the damping oil plug and communicated with the first area and the second area, the lower end of the damping oil plug is connected with a damping rod, one end of the damping rod, which is far away from the damping oil plug, is fixedly connected with an elastic buckle, and one end of the U-shaped frame, which is far away from the piston, is fixedly connected with a clamping plate;
when the elastic buckle is positioned in the area of the middle shaft, the elastic buckle and the clamping plate are mutually clamped, so that the elastic buckle can be driven to synchronously move downwards when the clamping plate moves downwards; when the elastic buckle is positioned in the area which is not in the intermediate shaft, the elastic buckle and the clamping plate are separated from each other to be clamped, and when the clamping plate moves downwards, the elastic buckle is not driven to move synchronously.
2. The device for testing the catalytic efficiency of the biological enzyme according to claim 1, further comprising a sticker, wherein one surface of the sticker is used for writing, the other surface of the sticker has adhesiveness, and the surface of the sticker having adhesiveness is adhered to the outer peripheral wall of the outer cylinder.
3. The device for testing the catalytic efficiency of the biological enzyme according to claim 1, wherein the shell is provided with a limit groove, the limit groove extends along the axial direction of the outer barrel, and the pen body of the line drawing pen is slidably connected in the limit groove.
4. A biological enzyme catalytic efficiency testing device according to claim 3, wherein an observation area is provided on one side of the housing in the circumferential direction.
5. The device for testing the catalytic efficiency of the biological enzyme according to claim 1, wherein a sealing plug is arranged at the top of the reaction container, and a substrate feeding pipe and a catalyst feeding pipe are inserted into the sealing plug.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202410071319.7A CN117586875B (en) | 2024-01-18 | 2024-01-18 | Biological enzyme catalytic efficiency testing arrangement |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202410071319.7A CN117586875B (en) | 2024-01-18 | 2024-01-18 | Biological enzyme catalytic efficiency testing arrangement |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN117586875A CN117586875A (en) | 2024-02-23 |
| CN117586875B true CN117586875B (en) | 2024-03-29 |
Family
ID=89915428
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202410071319.7A Active CN117586875B (en) | 2024-01-18 | 2024-01-18 | Biological enzyme catalytic efficiency testing arrangement |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN117586875B (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB190015211A (en) * | 1900-08-27 | 1901-08-10 | Thomas Thorp | A New and Improved Gas Pressure Recording Apparatus. |
| GB354852A (en) * | 1930-05-20 | 1931-08-20 | J W & C J Phillips Ltd | Improvements in or relating to apparatus for indicating and/or recording the change in volume of gases |
| GB652265A (en) * | 1946-06-14 | 1951-04-18 | Marcel Joseph Ernest Chopin | Apparatus for the analysis of fermentation |
| US4270381A (en) * | 1979-10-01 | 1981-06-02 | Demaray David E | Recording instrument to measure microbal gas production |
-
2024
- 2024-01-18 CN CN202410071319.7A patent/CN117586875B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB190015211A (en) * | 1900-08-27 | 1901-08-10 | Thomas Thorp | A New and Improved Gas Pressure Recording Apparatus. |
| GB354852A (en) * | 1930-05-20 | 1931-08-20 | J W & C J Phillips Ltd | Improvements in or relating to apparatus for indicating and/or recording the change in volume of gases |
| GB652265A (en) * | 1946-06-14 | 1951-04-18 | Marcel Joseph Ernest Chopin | Apparatus for the analysis of fermentation |
| US4270381A (en) * | 1979-10-01 | 1981-06-02 | Demaray David E | Recording instrument to measure microbal gas production |
Also Published As
| Publication number | Publication date |
|---|---|
| CN117586875A (en) | 2024-02-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN111473717A (en) | Automatic detection device for surface flatness of plate for electronic product | |
| CN201903364U (en) | Sensor capable of steady positioning | |
| CN117586875B (en) | Biological enzyme catalytic efficiency testing arrangement | |
| CN102095442A (en) | Self-adsorptive type sensor fixed device | |
| WO2003097251A3 (en) | Device for retaining and for inserting a flexible tube assembly into a fluid container | |
| CN217359826U (en) | Concentration detection device for paint production | |
| CN216535538U (en) | Pen for writable radiation developing ink | |
| CN215985336U (en) | Environment-friendly detection sampling device | |
| CN201298034Y (en) | An automatic liquid feeding titration device | |
| CN213262987U (en) | Bagging equipment for biomass material production | |
| CN209869861U (en) | Teaching compasses with length measurement | |
| CN202351089U (en) | ATP (Adenosine Triphosphate) fluorescence detection swab | |
| CN208953217U (en) | A kind of detection diesel vehicle pressure charging system gas leak detection device | |
| CN207113764U (en) | A kind of multifunctional drawing measuring scale | |
| CN220982577U (en) | Beer storage tank tightness detection device | |
| CN2247502Y (en) | Negative pressure type blood sampler | |
| CN218663076U (en) | Rapid detection kit convenient to use | |
| CN212242751U (en) | Writing pen capable of teaching | |
| CN218546042U (en) | Exhaust pipe leakage test tool | |
| CN221101067U (en) | Ecological exploration equipment of mining industry | |
| CN220824765U (en) | Water-based wood grain-like paint stirring device | |
| CN219606348U (en) | PVC pipe sleeve | |
| CN220819759U (en) | Impervious appearance is used in concrete quality testing | |
| CN212904934U (en) | Detection pen for qualitatively detecting colloidal gold product | |
| CN108420565B (en) | A kind of CO sucking models Hypoxia bottle |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |