CN117581859B - Embryo freezing and resuscitating device for equine animal cloning - Google Patents
Embryo freezing and resuscitating device for equine animal cloning Download PDFInfo
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- CN117581859B CN117581859B CN202410070033.7A CN202410070033A CN117581859B CN 117581859 B CN117581859 B CN 117581859B CN 202410070033 A CN202410070033 A CN 202410070033A CN 117581859 B CN117581859 B CN 117581859B
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- 238000007710 freezing Methods 0.000 title claims abstract description 58
- 230000008014 freezing Effects 0.000 title claims abstract description 58
- 210000001161 mammalian embryo Anatomy 0.000 title claims abstract description 49
- 241000283073 Equus caballus Species 0.000 title claims abstract description 29
- 238000010367 cloning Methods 0.000 title claims abstract description 26
- 230000005540 biological transmission Effects 0.000 claims abstract description 53
- 238000009434 installation Methods 0.000 claims abstract description 9
- 238000007789 sealing Methods 0.000 claims description 50
- 239000002184 metal Substances 0.000 claims description 10
- 238000003825 pressing Methods 0.000 claims description 9
- 230000009471 action Effects 0.000 claims description 6
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 4
- 239000000741 silica gel Substances 0.000 claims description 4
- 229910002027 silica gel Inorganic materials 0.000 claims description 4
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract description 74
- 239000007788 liquid Substances 0.000 abstract description 37
- 229910052757 nitrogen Inorganic materials 0.000 abstract description 37
- 230000008020 evaporation Effects 0.000 abstract description 7
- 238000001704 evaporation Methods 0.000 abstract description 7
- 230000000875 corresponding effect Effects 0.000 description 27
- 238000000034 method Methods 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 230000015572 biosynthetic process Effects 0.000 description 3
- 210000002257 embryonic structure Anatomy 0.000 description 3
- 230000035935 pregnancy Effects 0.000 description 3
- 241001494479 Pecora Species 0.000 description 2
- 241000282887 Suidae Species 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000006260 foam Substances 0.000 description 2
- 230000035882 stress Effects 0.000 description 2
- 238000004017 vitrification Methods 0.000 description 2
- 241000283086 Equidae Species 0.000 description 1
- 241000283074 Equus asinus Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 238000005299 abrasion Methods 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 238000012411 cloning technique Methods 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 238000002788 crimping Methods 0.000 description 1
- 238000005138 cryopreservation Methods 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000003028 elevating effect Effects 0.000 description 1
- 229920006248 expandable polystyrene Polymers 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000004880 explosion Methods 0.000 description 1
- 229920001973 fluoroelastomer Polymers 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0263—Non-refrigerated containers specially adapted for transporting or storing living parts whilst preserving, e.g. cool boxes, blood bags or "straws" for cryopreservation
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0263—Non-refrigerated containers specially adapted for transporting or storing living parts whilst preserving, e.g. cool boxes, blood bags or "straws" for cryopreservation
- A01N1/0268—Carriers for immersion in cryogenic fluid, both for slow-freezing and vitrification, e.g. open or closed "straws" for embryos, oocytes or semen
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/52—Mobile; Means for transporting the apparatus
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/85—Food storage or conservation, e.g. cooling or drying
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- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Mechanical Engineering (AREA)
- Dentistry (AREA)
- Chemical & Material Sciences (AREA)
- Environmental Sciences (AREA)
- Hematology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Clinical Laboratory Science (AREA)
- Microbiology (AREA)
- Sustainable Development (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention is applicable to the technical field of embryo freezing, and provides an embryo freezing and recovering device for equine animal cloning, which comprises the following components: jar body, apron and installation department, the installation department includes: the support column is fixedly connected with the cover plate; the transmission rod is rotationally connected with the support column, the driven wheel is fixedly connected on the transmission rod, and the support column is provided with a clamping assembly corresponding to the transmission rod; the movable plate is rotationally connected with the support column; the lifting block is in threaded connection with the transmission rod, and is provided with a pickup assembly which is used for fixing a carrier of an embryo to be frozen; the cover plate is provided with a plurality of through holes corresponding to the pick-up assembly. Therefore, the invention utilizes the mounting part to realize automatic placement and taking out of the embryo carrier in the liquid nitrogen tank, reduces the workload of operators, improves the utilization rate of human resources, ensures the minimum opening area by only opening corresponding through holes for placement and taking, reduces the evaporation of liquid nitrogen and has high safety.
Description
Technical Field
The invention relates to the technical field of embryo freezing, in particular to an embryo freezing and recovering device for equine animal cloning.
Background
Cloning techniques are not an example nationally, but cloned equines are generally known to be more versatile worldwide, as species themselves, the larger the volume, the more difficult the study. The pregnancy period of horses and donkeys is as long as 11-12 months, the pregnancy period of sheep is 5 months, and the longer the pregnancy period, the longer the time of pigs is, meaning that the time of waiting in the aspect of experiments is. Animals such as pigs and sheep can be studied for four to five months, and equine animals can take one year per experimental period. Therefore, the requirements for cryopreservation and resuscitation of fertilized embryos in equine animal cloning are more stringent.
The embryo-carrying rod is required to be operated below the liquid nitrogen surface in both the vitrification freezing process and the resuscitation process, so that the vitrification freezing technology has higher requirements on the operation level of workers, and the process of loading the embryo on the freezing carrying rod and throwing the embryo into the liquid nitrogen needs to be completed within 60 seconds, so that the time is urgent and the requirements are high. The process also needs manual operation, has high operation technology content, and can not realize automatic production temporarily. The process of placing and moving out the freezing carrying rod in the liquid nitrogen tank is relatively simple, but in the prior art, the freezing carrying rod still needs to be placed and moved out manually by a worker, so that the time for embryo loading by the worker is occupied, the embryo loading efficiency is reduced, the worker with a higher operation level does work with lower requirements, and the waste of human resources is caused. After the staff carries the pole freezing, need move the pole to the liquid nitrogen container in, need remove between laboratory bench and two stations of liquid nitrogen container this moment, dispersion operating personnel's energy, when manual opening liquid nitrogen container is placed or is taken out the pole that carries simultaneously, unable stability, timeliness and the security etc. of guaranteeing the operation, uncontrollable factor is more. Meanwhile, when the carrier rod is placed for multiple times, the liquid nitrogen tank needs to be opened for multiple times, and as the opening of the liquid nitrogen tank is fixed, the evaporation of liquid nitrogen is serious, and the loss of liquid nitrogen is large.
In summary, it is clear that the prior art has inconvenience and defects in practical use, so that improvement is needed.
Disclosure of Invention
Aiming at the defects, the invention aims to provide the embryo freezing and recovering device for the equine animal cloning, which utilizes the mounting part to realize automatic placement and taking out of embryo carriers in the liquid nitrogen tank, reduces uncontrollable factors caused by manual operation, simultaneously reduces the workload of operators, improves the utilization rate of human resources, subdivides the opening of the liquid nitrogen tank, only needs to open corresponding through holes for placement and taking, and reduces the evaporation of liquid nitrogen; the automatic positioning and placement of the carrier are realized by utilizing the relative movement among the movable rod, the transmission rod, the support column and the lifting block; the sealing block can be automatically opened or closed, meanwhile, the sealing block is elastically abutted to the through hole, and when the pressure in the liquid nitrogen tank is increased, the sealing block is ejected out, the pressure is released, and the safety is high.
In order to achieve the above object, the present invention provides an embryo freezing and resuscitating device for equine animal cloning, comprising: the device comprises a tank body, a cover plate and a mounting part, wherein the cover plate is in sealing connection with the tank body, and the mounting part is fixedly mounted on the cover plate; the carrier of embryo can be transported outside in the jar body to the installation department, the installation department includes:
the support column is fixedly connected with the cover plate; the top of the support column is provided with a control panel and a power supply, the power supply provides power, and the control panel controls the action sequence of the freezing and resuscitation device;
the transmission rod is rotationally connected with the support column, the driven wheel is fixedly connected on the transmission rod, the support column is provided with a clamping assembly corresponding to the transmission rod, and the clamping assembly can fixedly connect the transmission rod on the support column, so that the transmission rod can be fixed or rotated relative to the support seat;
the movable plate is rotationally connected with the support column, and the clamping assembly can fixedly connect the movable plate on the support column, so that the movable plate can be fixed or rotated relative to the support seat; the transmission rod and the movable plate can rotate relatively, a first servo motor is arranged on the movable plate, and the first servo motor is in transmission connection with the driven wheel through the driving wheel;
the lifting block is in threaded connection with the transmission rod, and is provided with a pickup assembly which is used for fixing a carrier of an embryo to be frozen;
the cover plate is provided with a plurality of through holes corresponding to the pick-up assembly, a sealing block is arranged inside the cover plate and is in sliding connection with the cover plate, the sealing block can seal the through holes, and when the lifting block drives the carrier to move up and down, the sealing block opens the corresponding through holes.
According to the embryo freezing and recovering device for equine animal cloning, one side of the lifting block is provided with the picking component, the other side of the lifting block is provided with the sliding rod, and the sliding rod is in sliding connection with the movable plate.
The embryo freezing and resuscitating device for equine animal cloning according to the present invention, the picking assembly comprises:
the picking block is in sliding connection with the lifting block, a clamping interface is arranged on the picking block and used for fixing a carrier, a metal block is arranged at the tail end of the carrier, and a first electromagnet is arranged on the clamping interface corresponding to the metal block;
and the second servo motor is fixedly connected with the lifting block and can drive the pickup block to move.
According to the embryo freezing and recovering device for equine animal cloning, the surface of the first electromagnet is provided with a silica gel layer.
According to the embryo freezing and recovering device for equine animal cloning, one side of the sealing block is provided with the chamfer structure, the cover plate is hollow, the sealing block is slidably connected in the cover plate, the cover plate is provided with the pressing plate corresponding to the chamfer structure, and the pressing plate is elastically connected with the cover plate.
According to the embryo freezing and recovering device for equine animal cloning, the cover plate is provided with the telescopic piece corresponding to the sealing block, one end of the telescopic piece is fixedly connected with the cover plate, and the other end of the telescopic piece is fixedly connected with the sealing block.
According to the embryo freezing and recovering device for equine animal cloning, the cover plate is provided with a plurality of trigger switches, and the trigger switches are arranged corresponding to the through holes.
According to the embryo freezing and recovering device for equine animal cloning, the telescopic piece is an electric cylinder.
The embryo freezing and recovering device for equine animal cloning according to the present invention, the clamping assembly comprises: the second electro-magnet and joint piece, joint piece elastic connection second electro-magnet, second electro-magnet are located the middle part of joint piece, second electro-magnet fixed connection the support column, the both sides of joint piece all are equipped with tooth form structure, transfer line and fly leaf all correspond the joint piece and are provided with tooth form structure.
According to the embryo freezing and recovering device for equine animal cloning, the bottom of the cover plate is provided with the buffer layer, and the buffer layer protrudes out of the through hole, so that the inner wall of the through hole is stepped.
The invention has the beneficial effects that:
according to the invention, the mounting part is utilized to realize automatic placement and taking-out of the embryo carrier in the liquid nitrogen tank, so that uncontrollable factors caused by manual operation are reduced, meanwhile, the workload of operators is reduced, the utilization rate of human resources is improved, the opening of the liquid nitrogen tank is subdivided, and the evaporation of liquid nitrogen is reduced by only opening corresponding through holes for placement and taking; the automatic positioning and placement of the carrier are realized by utilizing the relative movement among the movable rod, the transmission rod, the support column and the lifting block; the sealing block can be automatically opened or closed, meanwhile, the sealing block is elastically abutted to the through hole, and when the pressure in the liquid nitrogen tank is increased, the sealing block is ejected out, the pressure is released, and the safety is high.
Drawings
FIG. 1 is a schematic view of a three-dimensional mounting structure of the present invention; FIG. 2 is a schematic perspective sectional structure of the present invention; FIG. 3 is a schematic view of the structure of the cover plate of the present invention; FIG. 4 is a schematic view of the mounting structure of the clamping assembly of the present invention; FIG. 5 is a schematic view of the structure of the transmission rod of the present invention; FIG. 6 is a schematic view of a clip assembly according to the present invention; FIG. 7 is a schematic view of the structure of the snap-fit block of the present invention; FIG. 8 is a schematic view of the structure of the movable plate of the present invention; FIG. 9 is a schematic cross-sectional view of a movable plate according to the present invention; FIG. 10 is a schematic cross-sectional view of the cover plate and mounting portion of the present invention; FIG. 11 is a schematic view of the structure of the lifting block of the present invention; FIG. 12 is a schematic structural view of a carrier of the present invention; FIG. 13 is an enlarged schematic view of the detail A of FIG. 2; FIG. 14 is a schematic view of a first operational state of the clamping assembly of the present invention; FIG. 15 is a schematic view of a second operational configuration of the clamping assembly of the present invention; FIG. 16 is an enlarged schematic view of the detail B in FIG. 10; in the figure, 1-jar body, 11-heat preservation, 2-apron, 21-through hole, 22-extensible part, 23-sealing block, 24-clamp plate, 25-trigger switch, 26-spout, 27-buffer layer, 3-installation department, 31-support column, 311-recess, 32-transfer line, 321-follow driving wheel, 322-first tooth form structure, 33-fly leaf, 331-first servo motor, 332-guiding hole, 333-second tooth form structure, 334-action wheel, 34-lifting block, 341-pick up block, 3411-first electro-magnet, 342-second servo motor, 343-slide bar, 35-joint subassembly, 351-second electro-magnet, 352-joint block, 3521-arch, 353-spring, 4-carrier, 41-magnetic metal.
Detailed Description
The present invention will be described in further detail with reference to the drawings and examples, in order to make the objects, technical solutions and advantages of the present invention more apparent. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the invention.
Referring to fig. 1 to 16, the present invention provides an embryo freezing and resuscitating device for equine animal cloning. The embryo freezing and resuscitating device for equine animal cloning comprises: the device comprises a tank body 1, a cover plate 2 and a mounting part 3, wherein the cover plate 2 is in sealing connection with the tank body 1, and the mounting part 3 is fixedly mounted on the cover plate 2; the mounting portion 3 is capable of transferring the embryo carrier 4 inside and outside the can 1. The inner wall of the tank body 1 is provided with an insulating layer 11, and the mounting part 3 comprises: support column 31, transfer line 32, fly leaf 33 and elevating block 34. The carrier 4 is a freezing bracket, and the top of the freezing bracket is provided with a magnetic conductive metal 41.
A support column 31 fixedly connected to the cover plate 2; the top of support column 31 is equipped with control panel and power, and the power provides power, and control panel controls this freezing, the action sequence of resuscitation device, and control panel's internal program adopts PLC programming control, and the PLC programming belongs to prior art, and is not described in detail here.
The transmission rod 32 is rotationally connected with the support column 31, the driven wheel 321 is fixedly connected on the transmission rod 32, the support column 31 is provided with a clamping assembly 35 corresponding to the transmission rod 32, and the clamping assembly 35 can fixedly connect the transmission rod 32 on the support column 31, so that the transmission rod 32 can be fixed or rotated relative to the support seat.
The movable plate 33 is rotatably connected with the support column 31, and the clamping assembly 35 can fixedly connect the movable plate 33 on the support column 31, so that the movable plate 33 can be fixed or rotated relative to the support seat. The transmission rod 32 and the movable plate 33 can rotate relatively, a first servo motor 331 is arranged on the movable plate 33, and the first servo motor 331 is in transmission connection with the driven wheel 321 through a driving wheel 334; when the transmission rod 32 and the support column 31 are fixed by the clamping assembly 35, the movable plate 33 and the support column 31 can rotate relatively, whereas when the movable plate 33 and the support column 31 are fixed, the transmission rod 32 and the support column 31 can rotate relatively.
The lifting block 34 is in threaded connection with the transmission rod 32, and a pickup assembly is arranged on the lifting block 34 and is used for fixing the carrier 4 of the embryo to be frozen; when the transmission rod 32 rotates, the lifting block 34 moves up and down, so that the embryo carrier 4 is driven to enter the tank 1 or be taken out from the tank 1.
The cover plate 2 is provided with a plurality of through holes 21 corresponding to the pickup assembly, a sealing block 23 is arranged inside the cover plate 2, the sealing block 23 is connected with the cover plate 2 in a sliding mode, the cover plate 2 is provided with a sliding groove 26 corresponding to the sealing block 23, and the sealing block 23 can seal the through holes 21 so that the liquid nitrogen tank is sealed. When the lifting block 34 drives the carrier 4 to move up and down, the sealing block 23 opens the corresponding through hole 21, so that the carrier 4 can move up and down freely. When the carrier 4 moves up and down, only a single through hole 21 is opened, so that the evaporation amount of liquid nitrogen is reduced, and the loss of the liquid nitrogen is reduced.
Referring to fig. 8-11, further, a pickup assembly is disposed on one side of the lifting block 34, a sliding rod 343 is disposed on the other side of the lifting block 34, the sliding rod 343 is slidably connected with the movable plate 33, a guiding hole 332 is disposed on the movable plate 33 corresponding to the sliding rod 343, the sliding rod 343 is slidably connected with the guiding hole 332, and the sliding rod 343 ensures that the lifting block 34 does not rotate relative to the movable plate 33 when moving up and down. The pick-up assembly and the sliding rod 343 are respectively positioned at two sides of the lifting block 34, so that the stress at two sides of the lifting block 34 is balanced, and the unilateral abrasion of the lifting block 34 caused by unilateral stress is reduced.
Referring to fig. 11-13, further, the pick-up assembly includes: a pickup block 341 and a second servo motor 342. A picking block 341, which is slidingly connected with the lifting block 34, wherein a clamping interface is arranged on the picking block 341, the clamping interface is used for fixing the embryo carrier 4, a metal block is arranged at the tail end of the carrier 4, and a first electromagnet 3411 is arranged at the clamping interface corresponding to the metal block in combination with fig. 13; when the carrier 4 is required to be transported, the first electromagnet 3411 is electrified, the carrier 4 is adsorbed on the surface of the first electromagnet 3411, and the carrier 4 is picked up; when the carrier 4 needs to be placed in the can 1, the first electromagnet 3411 is powered off, and the carrier 4 falls into the can 1.
The second servo motor 342 is fixedly connected with the lifting block 34, and the second servo motor 342 can drive the pickup block 341 to move, so that the moving direction of the pickup block 341 is perpendicular to the axial direction of the support column 31. The second servo motor 342 is in threaded connection with the pickup block 341 through a screw, and rotation of the second servo motor 342 drives the pickup block 341 to move. The screw is rotatably connected with the lifting block 34.
Further, the surface of the first electromagnet 3411 is provided with a silica gel layer, and the silica gel layer can increase the buffer when the carrier 4 contacts with the first electromagnet 3411, so as to avoid the damage of the carrier 4 caused by impact.
Referring to fig. 16, further, a chamfer structure is provided on one side of the sealing block 23, the cover plate 2 is hollow, the sealing block 23 is slidably connected inside the cover plate 2, the cover plate 2 is provided with a pressing plate 24 corresponding to the chamfer structure, and the pressing plate 24 is elastically connected with the cover plate 2. The sealing block 23 is made of fluororubber, and has low temperature resistance, corrosion resistance, aging resistance and good elasticity. The clamp plate 24 crimping is on chamfer structure for sealing block 23 elasticity shutoff through-hole 21, when the pressure in jar body 1 increases, sealing block 23 atress warp, opens through-hole 21, makes the pressure release, improves sealed security, reduces the evaporation of liquid nitrogen simultaneously, reduces the risk of liquid nitrogen container explosion. The abutment force of the pressing plate 24 can be selectively determined by the specifications of the spring. The bottom surface of the pressing plate 24 is provided with an inclined surface corresponding to the chamfer structure, so that the pressing plate 24 is tightly attached to the sealing block 23.
Further, the cover plate 2 is provided with a telescopic member 22 corresponding to the sealing block 23, one end of the telescopic member 22 is fixedly connected with the cover plate 2, and the other end of the telescopic member 22 is fixedly connected with the sealing block 23. The telescopic member 22 controls the working state of the telescopic member 22 by using an external control system, and when the telescopic member 22 stretches, the sealing block 23 is driven to move, so that the through hole 21 is opened or closed.
Further, the cover plate 2 is provided with a plurality of trigger switches 25, and the trigger switches 25 are arranged corresponding to the through holes 21. When the carrier 4 triggers the trigger switch 25, the trigger switch 25 signals control the telescopic piece 22 to move, so that the sealing block 23 is automatically opened and closed. The trigger switch 25 may be a photoelectric trigger switch or a proximity switch.
Further, the telescopic member 22 may be an electric cylinder, an air cylinder or an electromagnet, and the telescopic member 22 controls the action state by means of an external control system. The electric cylinder is optimized, and the power source is a power supply, so that the electric cylinder is convenient to use.
Further, the clamping assembly 35 includes: the second electro-magnet 351 and joint piece 352, joint piece 352 elastic connection second electro-magnet 351, second electro-magnet 351 are located the middle part of joint piece 352, second electro-magnet 351 fixed connection support column 31, the both sides of joint piece 352 all are equipped with tooth form structure. For better description, the left side in fig. 6 is set to the left, and the right side is set to the right. Referring to fig. 6, the right tooth structure of the clamping block 352 is made of metal, so that the clamping block 352 moves leftwards when the second electromagnet 351 is electrified. The transmission rod 32 and the movable plate 33 are provided with a first tooth structure 322 and a second tooth structure 333 corresponding to the clamping block 352. The first tooth formation 322 and the second tooth formation 333 are capable of engaging with the tooth formation on the snap block 352. The clamping block 352 is connected with the support column 31 in a sliding mode, the clamping block 352 is provided with a protrusion 3521, the support column 31 is provided with a groove 311 corresponding to the protrusion 3521, the protrusion 3521 is connected with the groove 311 in a sliding mode, and the clamping block 352 is guaranteed to move along the radial direction of the support column 31. A spring 353 is provided between the engagement block 352 and the second electromagnet 351. The spring 353 makes the clamping block 352 normally abut against the movable plate 33.
Referring to fig. 14, in normal state, the second electromagnet 351 is powered off, and the right end of the clamping block 352 abuts against the movable plate 33 by virtue of the elastic force of the spring 353, so that the movable plate 33 and the support column 31 are relatively fixed, and the transmission rod 32 and the support column 31 can relatively rotate; referring to fig. 15, when the second electromagnet 351 is energized, the right end of the clamping block 352 is magnetically attracted to the surface of the electromagnet, so that the left end of the clamping block 352 is clamped to the driving rod 32, the right end of the clamping block 352 is separated from the movable plate 33, the driving rod 32 is relatively fixed to the support column 31, and the movable plate 33 and the support column 31 can relatively rotate. The transmission rod 32, the movable plate 33 and the support column 31 are fixed and released by the on-off control of the second electromagnet 351.
Referring to fig. 10, further, the bottom of the cover plate 2 is provided with a buffer layer 27, the buffer layer 27 is made of foamed polystyrene, the heat insulation effect of the liquid nitrogen tank is improved, the buffer layer 27 protrudes inside the through hole 21, the inner wall of the through hole 21 is stepped, and the cross-sectional area of each opening is further reduced. The size of the step is matched with the magnetic conductive metal 41 on the carrier 4, so that the carrier 4 can be hung on the step, and the carrier 4 is convenient to fix.
Referring to fig. 4-10, 14-15, when the pickup block 341 is required to rotate by a certain angle, the control system controls the second electromagnet 351 to be electrified, so that the clamping block 352 fixedly connects the transmission rod 32 with the support column 31, the first servo motor 331 is electrified, so that the driving wheel 334 rotates on the surface of the driven wheel 321, the movable plate 33 is driven to integrally rotate, the sliding rod 343 drives the lifting block 34 to synchronously rotate, so that the pickup block 341 rotates, then the second electromagnet 351 is powered off, so that the movable plate 33 is fixed with the support column 31, and the position of the pickup block 341 is kept fixed.
When the pickup block 341 is required to move up and down, the control system controls the second electromagnet 351 to be powered off, so that the clamping block 352 fixedly connects the movable plate 33 with the support column 31, the transmission rod 32 is in a free rotation state, the first servo motor 331 is powered on, and the driving wheel 334 drives the transmission rod 32 to rotate, so that the lifting block 34 moves up and down; the lifting block 34 can only move up and down due to the guiding action of the slide bar 343 and cannot rotate. The pickup block 341 is thus able to move up and down. The moving speed of the pickup block 341 can be controlled by the first servo motor 331 and the second servo motor 342.
When workers need to freeze embryos in batches, the embryos need to be loaded on a freezing carrier rod, then quickly put into a foam box filled with liquid nitrogen, and sleeved with an outer sleeve in the liquid nitrogen. At this time, a single carrying rod can be selected to be placed on the freezing bracket in sequence and then placed in a liquid nitrogen tank; alternatively, a plurality of embryo-loaded carrier bars may be temporarily placed in a liquid nitrogen foam box in batches, then the plurality of carrier bars may be collectively placed on a freezing support, and then the freezing support may be placed in a liquid nitrogen tank. When the freezing bracket is transferred into the liquid nitrogen tank, the freezing bracket is placed below the pickup block 341, the first electromagnet 3411 on the pickup block 341 is electrified, so that the freezing bracket is fixed on the pickup block 341, then the external control system controls the transmission rod 32, the movable plate 33 and the pickup block 341 to perform corresponding actions, so that the freezing bracket moves to the upper part of the appointed through hole 21, then the lifting block 34 moves downwards, the freezing bracket triggers the trigger switch 25, the telescopic piece 22 in the cover plate 2 moves, the sealing block 23 is driven to move, the through hole 21 is opened, the freezing bracket smoothly enters the through hole 21, at the moment, the first electromagnet 3411 is powered off, the freezing bracket is released, and the freezing bracket is hung on a step on the inner wall of the through hole 21, so that the freezing bracket is fixed. The choice of through-holes 21 can be controlled with a control system each time it is placed.
When an embryo needs to be resuscitated, the external control system controls the transmission rod 32, the movable plate 33 and the pickup block 341 to perform corresponding actions, so that the pickup block 341 moves to the position above the designated through hole 21, then the lifting block 34 moves downwards, the pickup block 341 triggers the trigger switch 25, the telescopic piece 22 in the cover plate 2 moves, the sealing block 23 is driven to move, the through hole 21 is opened, the pickup block 341 enters the through hole 21, at the moment, the first electromagnet 3411 is electrified, the freezing bracket is fixed by magnetic attraction, then the lifting block 34 moves upwards until the freezing bracket leaves the trigger range of the trigger switch 25, the telescopic piece 22 resets, the sealing block 23 resets and seals the through hole 21, and the sealing of the liquid nitrogen tank is realized. After the freezing bracket rises, the worker takes away the freezing bracket for subsequent operation. The placement and the taking out of the freezing bracket are automatically finished, uncontrollable factors caused by manual operation are reduced, the workload of operators is reduced, and the utilization rate of human resources is improved.
In summary, the present invention includes: the device comprises a tank body, a cover plate and a mounting part, wherein the cover plate is in sealing connection with the tank body, and the mounting part is fixedly mounted on the cover plate; the carrier of embryo can be transported outside in the jar body to the installation department, the installation department includes: the support column is fixedly connected with the cover plate; the top of the support column is provided with a control panel and a power supply, the power supply provides power, and the control panel controls the action sequence of the freezing and resuscitation device; the transmission rod is rotationally connected with the support column, the driven wheel is fixedly connected on the transmission rod, the support column is provided with a clamping assembly corresponding to the transmission rod, and the clamping assembly can fixedly connect the transmission rod on the support column, so that the transmission rod can be fixed or rotated relative to the support seat; the movable plate is rotationally connected with the support column, and the clamping assembly can fixedly connect the movable plate on the support column, so that the movable plate can be fixed or rotated relative to the support seat; the transmission rod and the movable plate can rotate relatively, a first servo motor is arranged on the movable plate, and the first servo motor is in transmission connection with the driven wheel through the driving wheel; the lifting block is in threaded connection with the transmission rod, and is provided with a pickup assembly which is used for fixing a carrier of an embryo to be frozen; the cover plate is provided with a plurality of through holes corresponding to the pick-up assembly, a sealing block is arranged in the cover plate and is in sliding connection with the cover plate, the sealing block can seal the through holes, and when the lifting block drives the carrier to move up and down, the sealing block opens the corresponding through holes; according to the invention, the mounting part is utilized to realize automatic placement and taking-out of the embryo carrier in the liquid nitrogen tank, so that uncontrollable factors caused by manual operation are reduced, meanwhile, the workload of operators is reduced, the utilization rate of human resources is improved, the opening of the liquid nitrogen tank is subdivided, the placement and taking-out only need to open corresponding through holes, the minimum opening area is ensured, and the evaporation of liquid nitrogen is reduced; the automatic positioning and placement of the carrier are realized by utilizing the relative movement among the movable rod, the transmission rod, the support column and the lifting block; the sealing block can be automatically opened or closed, meanwhile, the sealing block is elastically abutted to the through hole, and when the pressure in the liquid nitrogen tank is increased, the sealing block is ejected out, the pressure is released, and the safety is high.
In the description of the present invention, it should be understood that the directions or positional relationships indicated by the terms "upper", "lower", "left", "right", etc. are based on the directions or positional relationships shown in the drawings, are merely for convenience of describing the present invention and simplifying the description, and do not indicate or imply that the apparatus or element in question must be provided with a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as limiting the present invention.
Of course, the present invention is capable of other various embodiments and its several details are capable of modification and variation in light of the present invention, as will be apparent to those skilled in the art, without departing from the spirit and scope of the invention as defined in the appended claims.
Claims (9)
1. An embryo freezing and resuscitating device for equine animal cloning, comprising: the device comprises a tank body, a cover plate and a mounting part, wherein the cover plate is in sealing connection with the tank body, and the mounting part is fixedly mounted on the cover plate; the carrier of embryo can be transported outside in the jar body to the installation department, the installation department includes:
the support column is fixedly connected with the cover plate; the top of the support column is provided with a control panel and a power supply, the power supply provides power, and the control panel controls the action sequence of the freezing and resuscitation device;
the transmission rod is rotationally connected with the support column, the driven wheel is fixedly connected on the transmission rod, the support column is provided with a clamping assembly corresponding to the transmission rod, and the clamping assembly can fixedly connect the transmission rod on the support column, so that the transmission rod can be fixed or rotated relative to the support seat;
the movable plate is rotationally connected with the support column, and the clamping assembly can fixedly connect the movable plate on the support column, so that the movable plate can be fixed or rotated relative to the support seat; the transmission rod and the movable plate can rotate relatively, a first servo motor is arranged on the movable plate, and the first servo motor is in transmission connection with the driven wheel through the driving wheel;
the lifting block is in threaded connection with the transmission rod, and is provided with a pickup assembly which is used for fixing a carrier of an embryo to be frozen;
the cover plate is provided with a plurality of through holes corresponding to the pick-up assembly, a sealing block is arranged in the cover plate and is in sliding connection with the cover plate, the sealing block can seal the through holes, and when the lifting block drives the carrier to move up and down, the sealing block opens the corresponding through holes;
the clamping assembly comprises: the second electromagnet is elastically connected with the clamping block, the second electromagnet is positioned in the middle of the clamping block, the second electromagnet is fixedly connected with the supporting column, tooth-shaped structures are arranged on two sides of the clamping block, and the transmission rod and the movable plate are provided with tooth-shaped structures corresponding to the clamping block;
the transmission rod and the movable plate are provided with a first tooth-shaped structure and a second tooth-shaped structure corresponding to the clamping block; the first tooth-shaped structure and the second tooth-shaped structure can be meshed with the tooth-shaped structure on the clamping block; the clamping block is in sliding connection with the support column, the clamping block is provided with a protrusion, the support column is provided with a groove corresponding to the protrusion, the protrusion is in sliding connection with the groove, and the clamping block is ensured to move along the radial direction of the support column; a spring is arranged between the clamping block and the second electromagnet; the spring enables the clamping block to be abutted on the movable plate in a normal state.
2. The embryo freezing and resuscitation device for equine animal cloning of claim 1, wherein a pickup assembly is provided on one side of the lifting block, and a slide bar is provided on the other side of the lifting block, and the slide bar is slidably connected to the movable plate.
3. The equine cloned embryo freezing and resuscitation device of claim 2, wherein the pick-up assembly comprises:
the picking block is in sliding connection with the lifting block, a clamping interface is arranged on the picking block and used for fixing a carrier, a metal block is arranged at the tail end of the carrier, and a first electromagnet is arranged on the clamping interface corresponding to the metal block;
and the second servo motor is fixedly connected with the lifting block and can drive the pickup block to move.
4. The embryo freezing and resuscitating device for equine animal cloning according to claim 3, wherein the surface of the first electromagnet is provided with a silica gel layer.
5. The embryo freezing and recovering device for equine animal cloning according to claim 4, wherein a chamfer structure is provided on one side of the sealing block, the cover plate is hollow, the sealing block is slidably connected inside the cover plate, the cover plate is provided with a pressing plate corresponding to the chamfer structure, and the pressing plate is elastically connected with the cover plate.
6. The embryo freezing and resuscitation device for equine animal cloning of claim 5, wherein the cover plate is provided with a telescopic member corresponding to the sealing block, one end of the telescopic member is fixedly connected with the cover plate, and the other end of the telescopic member is fixedly connected with the sealing block.
7. The embryo freezing and resuscitation device for equine animal cloning of claim 6, wherein a plurality of trigger switches are provided on the cover plate, and the trigger switches are provided corresponding to the through holes.
8. The embryo freezing and resuscitation device for equine animal cloning of claim 7, wherein said telescoping member is an electric cylinder.
9. The embryo freezing and resuscitating device for equine animal cloning according to claim 8, wherein the bottom of the cover plate is provided with a buffer layer protruding inside the through hole so that the inner wall of the through hole is stepped.
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