CN117572623A - Double-objective integrated optical tweezers system and operation method thereof - Google Patents
Double-objective integrated optical tweezers system and operation method thereof Download PDFInfo
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- CN117572623A CN117572623A CN202311854171.6A CN202311854171A CN117572623A CN 117572623 A CN117572623 A CN 117572623A CN 202311854171 A CN202311854171 A CN 202311854171A CN 117572623 A CN117572623 A CN 117572623A
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- 238000012576 optical tweezer Methods 0.000 title claims abstract description 48
- 238000000034 method Methods 0.000 title claims abstract description 23
- 238000003384 imaging method Methods 0.000 claims abstract description 59
- 238000005286 illumination Methods 0.000 claims abstract description 13
- 230000003287 optical effect Effects 0.000 claims description 43
- 238000013519 translation Methods 0.000 claims description 16
- 230000009977 dual effect Effects 0.000 claims description 14
- 239000002245 particle Substances 0.000 claims description 13
- 238000006073 displacement reaction Methods 0.000 claims description 11
- 239000011521 glass Substances 0.000 claims description 9
- 229910001220 stainless steel Inorganic materials 0.000 claims description 7
- 239000010935 stainless steel Substances 0.000 claims description 7
- 230000009471 action Effects 0.000 claims description 6
- 238000003825 pressing Methods 0.000 claims description 5
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- 230000000694 effects Effects 0.000 claims description 4
- 239000013307 optical fiber Substances 0.000 claims description 4
- 238000002360 preparation method Methods 0.000 claims description 4
- 238000012216 screening Methods 0.000 claims description 3
- 238000001228 spectrum Methods 0.000 claims description 3
- 238000009434 installation Methods 0.000 claims 2
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- G—PHYSICS
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- G02B21/00—Microscopes
- G02B21/32—Micromanipulators structurally combined with microscopes
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/34—Microscope slides, e.g. mounting specimens on microscope slides
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
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Abstract
The invention discloses a double-objective integrated optical tweezers system and an operation method thereof; the device comprises an illumination module, a forward microscopic imaging module, a sample placement module, an inverted microscopic imaging module, a laser module and a bracket structure, wherein the components of the device are built by a multi-axis cage structure; the invention is built by adopting a multi-axis cage structure, and all mechanical structure devices are connected into a whole, so that the stability of the whole device is greatly enhanced, and the invention is easier to popularize in the market; the device uses the low-magnification air objective lens as a focusing and observing objective lens, can provide a larger sample space and is suitable for accommodating containers with various specifications; the system light path adopts a multi-axis multiplexing mode, so that the whole occupied space is reduced, and the integration level of the system is improved; the near infrared laser used by the system has less damage to biological tissues and cells, and is suitable for performing living biological tissue operation; the system adopts a semi-quantitative adjusting mode, and the illumination of the sample is very uniform, so that clear microscopic images can be conveniently acquired.
Description
Technical Field
The invention relates to the technical field of optical tweezers and microscopes, in particular to a double-objective integrated optical tweezers system and an operation method thereof.
Background
Light has energy and momentum, and since the energy of a single photon is extremely weak, we cannot see the phenomenon that light moves particles when it acts with them. Until the 60 s of the 20 th century, the invention of laser provides a brand-new light source for human study of the interaction between light and substances, and the optical tweezers technology is only developed. Optical tweezers, i.e. optical tweezers, are a physical tool based on the mechanical effect of laser light, being an optical potential well formed by means of a strongly converging laser beam. When particles enter the range of the optical trap, the particles are restrained at the optical trap under the action of light pressure, and the micro objects can be controlled by moving the light beam along with the movement of the optical trap, so that the interaction of light and substances is achieved. With the development of optical tweezers, precise operations on sub-nanometer cells are currently being performed. The optical tweezers have the advantages that the non-contact mode avoids injury and pollution, and meanwhile, the accurate application and detection of force can be performed.
The objective lens used by the traditional optical tweezers system is mostly an oil immersion microscope objective lens with high multiplying power and high NA, refractive index oil matched with the objective lens needs to be added between a cover glass and the objective lens before the objective lens is used, residual refractive index oil on the objective lens needs to be cleaned in time after the experiment is finished, the operation process is complex, the traditional optical tweezers system is not suitable for carrying out classroom experiment operation, the traditional optical tweezers system is difficult to collect transmission images and reflection images of particle movement in real time, the stability of the optical tweezers system built in a laboratory is insufficient, the building process is complex, the building achievement is complex, batch copying is difficult to carry out, and the optical tweezers system is more difficult to popularize in the market.
Disclosure of Invention
The invention aims to provide a double-objective integrated optical tweezers system and an operation method thereof, which are used for solving the problems in the background technology.
In order to solve the technical problems, the invention provides the following technical scheme: the double-objective integrated optical tweezers system comprises an illumination module, a positive microscopic imaging module, a sample placement module, an inverted microscopic imaging module, a laser module and a bracket structure, wherein the components of the system are built by a multi-axis cage structure; the method comprises the steps of carrying out a first treatment on the surface of the
The support structure comprises a vertical support structure and a horizontal support structure;
the illumination module is arranged on the front microscopic imaging module;
the upright microscopic imaging module is arranged on the vertical support structure;
the inverted microscopic imaging module is arranged on the horizontal bracket structure and is positioned below the upright microscopic imaging module;
the sample placing module is arranged on the horizontal bracket structure and is partially arranged between the upright microscopic imaging module and the inverted microscopic imaging module;
the laser module is arranged on the horizontal support structure and is used for forming an optical trap of the optical tweezers by laser emitted by the optical fiber.
Preferably, the horizontal support structure is a 300mm×450mm optical flat plate one, the vertical support structure comprises a column fixing block, a stainless steel column, a one-dimensional translation table, a 100mm×150mm optical flat plate two and a multifunctional adapter, the column fixing block is fixed on the optical flat plate one, the multifunctional adapter is sleeved on the stainless steel column, the one-dimensional translation table is mounted on the front surface of the multifunctional adapter, the optical flat plate two is mounted on the front surface of the one-dimensional translation table, and the overhead microscopic imaging module and the illumination module are fixed on the optical flat plate two through extension parts of a multi-axis cage structure and move along a z axis under the action of the one-dimensional translation table.
Preferably, the lighting module comprises a light source, a collimating lens and a converging lens which are sequentially connected, wherein the light source is an LED (light emitting diode) light source capable of triggering white light outwards, and the light source, the collimating lens and the converging lens are respectively clamped in the multi-axis cage structure.
Preferably, the front microscopic imaging module comprises a first camera, a first shading sleeve, a first lens barrel lens, a first optical filter, a first beam splitter, a two-dimensional adjusting frame and a first objective lens which are sequentially connected from top to bottom, and is fixedly connected by the multi-axis cage structure, and the illumination module is connected to the side face of the first beam splitter and is vertically distributed with the front microscopic imaging module.
Preferably, the sample placement module comprises a rough adjustment flat plate, a three-dimensional fine adjustment displacement table and a sample frame which are sequentially installed from bottom to top, and the rough adjustment flat plate is installed on the horizontal support structure.
Preferably, a sample clamping device is arranged on the sample rack, and the sample clamping device is arranged on one side of the sample rack and is positioned between the upright microscopic imaging module and the inverted microscopic imaging module.
Preferably, the inverted microscopic imaging module sequentially comprises a second objective lens, a first reflecting mirror, a beam splitter, a second reflecting mirror, a second optical filter, a second lens barrel lens, a second shading sleeve and a second camera from left to right along an x-axis, wherein the second reflecting mirror is arranged above the second beam splitter, the second optical filter, the second lens barrel lens, the second shading sleeve and the second camera are all erected above the laser module, and the laser module is arranged on one side of the second beam splitter.
Preferably, the laser module sequentially comprises a collimating lens, a beam expander and a laser along the x-axis from left to right, and is clamped in the multi-axis cage structure as a whole, and the laser projection light source is near infrared laser.
Preferably, the upright microscopic imaging module and the inverted microscopic imaging module are both provided with low-magnification air objective lenses.
An operation method based on a double-objective integrated optical tweezer system comprises the following steps:
s1, sample preparation: dropping the diluted particulate solution into the grooves of the grooved slide, so as to prevent the solution from volatilizing during operation, covering the solution with a cover slip and wiping off excess residual solution; the method comprises the steps of carrying out a first treatment on the surface of the
S2, sample loading: clamping the prepared glass slide on a sample frame, clamping and pressing the pressing sheet on the areas without samples at the two sides of the glass slide, and roughly adjusting the grooves of the glass slide to the center of the optical axis of the objective lens; the method comprises the steps of carrying out a first treatment on the surface of the
S3, focusing: firstly, connecting two cameras with a computer, opening camera software, opening a light source after the cameras normally run, and adjusting exposure compensation of the camera software to ensure that the cameras have no overexposure condition; then finding out an image of the inverted microscopic module, and rotating a Z-axis knob of the sample stage according to the definition degree of the image to enable the image to be clear; and finally, finding out an image of the upright microscopic module, rotating a knob of the one-dimensional displacement table to make the image clear, and completing focusing of the system. The method comprises the steps of carrying out a first treatment on the surface of the
S4, capturing and operating the optical tweezers: the laser is turned on, the position of a light spot where laser is converged is found in a sample image, the X-axis knob and the Y-axis knob of the sample stage are moved to the vicinity of particles needing to be controlled, the particles under the action of light pressure can be pushed to the light spot and are bound at the optical trap, and at the moment, displacement control, screening, spectrum research and the like can be carried out on the bound particles.
Compared with the prior art, the invention has the beneficial effects that:
1. the optical tweezers system built by adopting the multi-axis cage structure has the advantages that all mechanical structure devices are connected into a whole, the instability problem caused by independent placement of a single device is reduced, and the stability of the whole device is greatly enhanced;
2. the invention uses the low-magnification air objective lens as the focusing and observing objective lens of the optical tweezers system, can provide larger sample space, is suitable for accommodating containers with various specifications, and is simple in sample preparation and suitable for carrying out classroom experiment operation;
3. the optical path adopts a multi-axis multiplexing mode, so that the optical paths are multiplexed in the same horizontal space, the occupied space of the whole system is reduced, and the integration level of the system is improved;
4. the system uses near infrared laser as an operation light source of the optical tweezers, and the laser of the wave band has small damage to biological tissues and cells, thereby being suitable for performing living biological tissue operation
5. The whole system adopts a semi-quantitative adjusting mode in the adjusting process, the illumination of the system to the sample is very uniform, and clear microscopic images can be acquired; meanwhile, due to the stability of the system structure, the operation of the optical tweezers is stable, the using effect is obviously improved, and the system can be upgraded and improved under the condition that the original structure is unchanged, so that the optical tweezers have strong expansibility and are easy to popularize in the market.
Drawings
FIG. 1 is a schematic diagram of the overall structure of a dual objective integrated optical tweezers system and method of operation thereof according to the present invention;
FIG. 2 is a schematic view of an illumination and forward microscopic imaging module of a dual objective integrated optical tweezers system and method of operation thereof according to the present invention;
FIG. 3 is a schematic diagram of a laser and inverted microscopic imaging module of a dual objective integrated optical tweezers system and method of operation thereof according to the present invention;
fig. 4 is a schematic diagram of a sample placement module of a dual objective integrated optical tweezers system and a method for operating the same according to the present invention.
In the figure: 1. a lighting module; 101. a light source; 102. a collimating lens; 103. a converging lens; 2. a positive microscopic imaging module; 201. a first camera; 202. a first shading sleeve; 203. a first lens barrel lens; 204. a first optical filter; 205. a first beam splitter; 206. a two-dimensional adjusting frame; 207. an objective lens I; 3. a sample placement module; 301. a rough adjustment plate; 302. a three-dimensional fine adjustment displacement table; 303. a sample holder; 304. a sample holding device; 4. an inverted microimaging module; 401. an objective lens II; 402. a first reflecting mirror; 403. a beam splitter II; 404. a second reflecting mirror; 405. a second optical filter; 406. a second lens barrel lens; 407. a second shading sleeve; 408. a second camera; 5. a laser module; 501. a collimator lens; 502. a beam expander; 503. a laser; 6. a support structure; 601. a column fixing block; 602. stainless steel upright posts; 603. a one-dimensional translation stage; 604. an optical flat II; 605. an optical flat I; 606. a multifunctional adapter; 7. multiaxial cage structure.
Detailed Description
The invention is further described in connection with the following detailed description, in order to make the technical means, the creation characteristics, the achievement of the purpose and the effect of the invention easy to understand.
Referring to fig. 1-4, a dual objective integrated optical tweezers system includes an illumination module 1, a front microscopic imaging module 2, a sample placement module 3, an inverted microscopic imaging module 4, a laser module 5 and a support structure 6, wherein the components are built by a multi-axis cage structure 7; the method comprises the steps of carrying out a first treatment on the surface of the
Specifically, the support structure 6 includes a vertical support structure and a horizontal support structure;
specifically, the illumination module 1 is mounted on the front microscopic imaging module 2;
specifically, the front microscopic imaging module 2 is mounted on a vertical support structure;
specifically, the inverted microimaging module 4 is mounted on a horizontal support structure and is located below the upright microimaging module 2;
specifically, the sample placement module 3 is mounted on a horizontal bracket structure and is partially arranged between the upright microscopic imaging module 2 and the inverted microscopic imaging module 4;
specifically, the laser module 5 is mounted on a horizontal support structure, and is used to form the laser light emitted from the optical fiber into an optical trap of the optical tweezers.
According to the invention, the whole structure frame is built by a multi-axis cage structure, all modules are connected by mechanical devices, all supporting devices in the structure can be connected into a whole, an optical flat plate is used as a unique reference surface, coaxiality and stability of the system are guaranteed, the whole system can be transported for a long distance, the whole structure is compact and stable, the use is convenient, and the multi-axis cage structure is concise and stable, so that the multi-axis cage structure can be copied in batches and is easy to popularize in the market.
Referring to fig. 1 to 3, the horizontal support structure is a 300mm×450mm optical flat 605, the vertical support structure includes a column fixing block 601, a stainless steel column 602, a one-dimensional translation stage 603, a 100mm×150mm optical flat 604 and a multifunctional adapter 606, the column fixing block 601 is fixed on the optical flat 605, the multifunctional adapter 606 is sleeved on the stainless steel column 602, the front surface of the column fixing block is provided with a one-dimensional translation stage 603, the front surface of the one-dimensional translation stage 603 is provided with a second optical flat 604, and the upright microscopic imaging module 2 and the lighting module 1 are fixed on the second optical flat 604 through the extension part of the multi-axis cage structure 7 and move along the z axis under the action of the one-dimensional translation stage 603.
Referring to fig. 2, the lighting module 1 includes a light source 103, a collimating lens 102 and a converging lens 101 sequentially connected, the light source 103 is an LED light source capable of triggering white light, and the light source 103, the collimating lens 102 and the converging lens 101 are respectively clamped in a single-hole sliding frame of the multi-axis cage structure 7 and are connected in series to form a whole through a cage rod of cemented carbide.
Specifically, the front-mounted microscopic imaging module 2 comprises a first camera 201, a first shading sleeve 202, a first lens barrel lens 203, a first optical filter 204, a first beam splitter 205, a two-dimensional adjusting frame 206 and a first objective 207 which are sequentially connected from top to bottom, and is fixedly connected through a multi-axis cage structure 7, the lighting module 1 is connected to the side face of the first beam splitter 205 and is vertically distributed with the front-mounted microscopic imaging module 2, the whole front-mounted microscopic imaging module 2 is fixed on a vertically placed one-dimensional translation table 603, movement focusing in the Z-axis direction can be carried out on the whole front-mounted microscopic imaging module 2, and the reflected images of samples can be collected conveniently.
Referring to fig. 4, the sample placement module 3 includes a rough adjustment plate 301, a three-dimensional fine adjustment displacement platform 302 and a sample holder 303, which are sequentially installed from bottom to top, wherein the rough adjustment plate 301 is installed on a horizontal bracket structure, and can clamp sample containers with various specifications, so as to provide rough and fine adjustment movement functions for samples.
Specifically, a sample holder 303 is provided with a sample holding device 304, and the sample holding device 304 is disposed on one side of the sample holder 303 and between the upright microscopic imaging module 2 and the inverted microscopic imaging module 4.
Referring to fig. 3, the inverted microscopic imaging module 4 sequentially includes, from left to right along the x-axis, a second objective 401, a first mirror 402, a second beam splitter 403, a second mirror 404, a second filter 405, a second barrel lens 406, a second light-shielding sleeve 407, and a second camera 408, where the second mirror 404 is installed above the second beam splitter 403, the second filter 405, the second barrel lens 406, the second light-shielding sleeve 407, and the second camera 408 are all erected above the laser module 5, and the laser module 5 is installed on one side of the second beam splitter 403, and the components in the inverted microscopic imaging module 4 are also clamped in corresponding clamping devices, and are connected into a whole by a cage bar of cemented carbide, and in operation, a transmission image of the sample is collected.
Specifically, the laser module 5 sequentially comprises a collimating mirror 501, a beam expander 502 and a laser 503 from left to right along the x-axis, and is clamped in the multi-axis cage structure 7 as a whole, the laser 503 projects a near infrared laser, the module collimates and expands the laser emitted by the optical fiber to form an optical trap of the optical tweezers, and the near infrared laser can protect living cells to a greater extent, so that experimental success is ensured.
Specifically, a low-magnification air objective lens is adopted in both the upright microscopic imaging module 2 and the inverted microscopic imaging module 4.
An operation method based on a double-objective integrated optical tweezer system comprises the following steps:
s1, sample preparation: dropping the diluted particulate solution into the grooves of the grooved slide, so as to prevent the solution from volatilizing during operation, covering the solution with a cover slip and wiping off excess residual solution; the method comprises the steps of carrying out a first treatment on the surface of the
S2, sample loading: clamping the prepared glass slide on a sample frame 303, clamping the pressing sheet on the areas without samples on the two sides of the glass slide, and roughly adjusting the grooves of the glass slide to the center of the optical axis of the objective lens; the method comprises the steps of carrying out a first treatment on the surface of the
S3, focusing: firstly, connecting a first camera 201 and a second camera 408 with a computer, opening camera software, opening a light source 103 after the cameras normally run, and adjusting exposure compensation of the camera software to enable the cameras not to be overexposed; then finding out an image of the inverted microscopic imaging module 4, and rotating a Z-axis knob of the sample stage according to the definition degree of the image to enable the image to be clear; finally, the image of the front microscopic imaging module 2 is found, and the knob of the one-dimensional translation table 603 is rotated to make the image clear, so that the system focusing is completed. The method comprises the steps of carrying out a first treatment on the surface of the
S4, capturing and operating the optical tweezers: the laser 503 is turned on, the position of the light spot where the laser converges is found in the sample image, the X-axis and Y-axis knobs of the three-dimensional fine tuning displacement table 302 are moved to the vicinity of the particles to be controlled, the particles under the action of light pressure are pushed to the light spot and bound at the optical trap, and at this time, the bound particles can be subjected to displacement control, screening, spectrum research and the like.
The dual-objective integrated optical tweezers system has the advantages of high integration degree, stable system structure, large expandable space and the like, so that the dual-objective integrated optical tweezers system is widely applicable to groups, can be used as a scientific research tool for universities and scientific research institutions, and can also be used as a course experiment system for the professions of photoelectric information, electromechanical integration, physics and the like.
The system can check the assembly of the mechanical structure of a user, the light path debugging, the debugging of the electric displacement table, the acquisition and the writing capability of a control program, and realize talent culture of interdisciplinary.
The foregoing has shown and described the basic principles and main features of the present invention and the advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, and that the above embodiments and descriptions are merely illustrative of the principles of the present invention, and various changes and modifications may be made without departing from the spirit and scope of the invention, which is defined in the appended claims. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (10)
1. The utility model provides a two objective integrated optical tweezers system which characterized in that: the device comprises an illumination module (1), a front microscopic imaging module (2), a sample placement module (3), an inverted microscopic imaging module (4), a laser module (5) and a bracket structure (6), wherein the components of the device are built by a multi-axis cage structure (7);
the support structure (6) comprises a vertical support structure and a horizontal support structure;
the illumination module (1) is arranged on the front microscopic imaging module (2);
the upright microscopic imaging module (2) is arranged on the vertical support structure;
the inverted microscopic imaging module (4) is arranged on the horizontal bracket structure and is positioned below the upright microscopic imaging module (2);
the sample placement module (3) is arranged on a horizontal bracket structure and is partially arranged between the upright microscopic imaging module (2) and the inverted microscopic imaging module (4);
the laser module (5) is arranged on the horizontal support structure and is used for forming an optical trap of the optical tweezers by laser emitted by the optical fiber.
2. The dual objective integrated optical tweezers system of claim 1, wherein: the horizontal support structure is 300mm×450 mm's optical flat (605), the vertical support structure includes stand fixed block (601), stainless steel stand (602), one-dimensional translation platform (603), 100mm×150 mm's optical flat (604) and multi-functional adaptor (606), stand fixed block (601) is fixed on optical flat (605), multi-functional adaptor (606) cover is established on stainless steel stand (602), and its front installation one-dimensional translation platform (603), one-dimensional translation platform (603) front installation optical flat (604), overhead microscopic imaging module (2) and lighting module (1) are all fixed on optical flat (604) through the extension of multiaxis cage structure (7), and move along the z axle under the effect of one-dimensional translation platform (603).
3. The dual objective integrated optical tweezers system of claim 1, wherein: the lighting module (1) comprises a light source (103), a collimating lens (102) and a converging lens (101) which are sequentially connected, wherein the light source (103) is an LED (light-emitting diode) externally triggerable white light source, and the light source (103), the collimating lens (102) and the converging lens (101) are respectively clamped in the multi-axis cage structure (7).
4. The dual objective integrated optical tweezers system of claim 1, wherein: the front microscopic imaging module (2) comprises a first camera (201), a first shading sleeve (202), a first lens barrel lens (203), a first optical filter (204), a first beam splitter (205), a two-dimensional adjusting frame (206) and a first objective lens (207) which are sequentially connected from top to bottom, the front microscopic imaging module is fixedly connected with the multi-axis cage structure (7), and the illumination module (1) is connected to the side face of the first beam splitter (205) and is vertically distributed with the front microscopic imaging module (2).
5. The dual objective integrated optical tweezers system of claim 1, wherein: the sample placement module (3) comprises a rough adjustment flat plate (301), a three-dimensional fine adjustment displacement table (302) and a sample frame (303) which are sequentially installed from bottom to top, and the rough adjustment flat plate (301) is installed on the horizontal support structure.
6. The dual objective integrated optical tweezers system of claim 5, wherein: the sample holder (303) is provided with a sample clamping device (304), and the sample clamping device (304) is arranged on one side of the sample holder (303) and is positioned between the upright microscopic imaging module (2) and the inverted microscopic imaging module (4).
7. The dual objective integrated optical tweezers system of claim 1, wherein: the inverted microscopic imaging module (4) sequentially comprises a second objective lens (401), a first reflecting mirror (402), a second beam splitter (403), a second reflecting mirror (404), a second optical filter (405), a second lens barrel lens (406), a second shading sleeve (407) and a second camera (408) from left to right along an x-axis, wherein the second reflecting mirror (404) is installed above the second beam splitter (403), and the second optical filter (405), the second lens barrel lens (406), the second shading sleeve (407) and the second camera (408) are all erected above the laser module (5), and the laser module (5) is installed on one side of the second beam splitter (403).
8. The dual objective integrated optical tweezers system of claim 1, wherein: the laser module (5) sequentially comprises a collimating lens (501), a beam expanding lens (502) and a laser (503) from left to right along an x-axis, and is clamped in the multi-axis cage structure (7) into a whole, and a projection light source of the laser (503) is near infrared laser.
9. The dual objective integrated optical tweezers system of claim 1, wherein: the upright microscopic imaging module (2) and the inverted microscopic imaging module (4) are respectively provided with a low-magnification air objective lens.
10. A method of operating a dual objective integrated optical tweezers system according to any one of claims 1-9, characterized in that: comprising the following steps:
s1, sample preparation: dropping the diluted particulate solution into the grooves of the grooved slide, so as to prevent the solution from volatilizing during operation, covering the solution with a cover slip and wiping off excess residual solution;
s2, sample loading: clamping the prepared glass slide on a sample frame (303), clamping and pressing a pressing sheet on the areas without samples at the two sides of the glass slide, and roughly adjusting the grooves of the glass slide to the center of the optical axis of the objective lens;
s3, focusing: firstly, connecting a first camera (201) and a second camera (408) with a computer, opening camera software, opening a light source (103) after the cameras are normally operated, adjusting exposure compensation of the camera software to enable the cameras not to be overexposed, then finding out an image of an inverted microscopic imaging module (4), rotating a Z-axis knob of a sample stage according to the definition degree of the image to enable the image to be clear, finally finding out an image of an upright microscopic imaging module (2), rotating a knob of a one-dimensional translation stage (603) to enable the image to be clear, and completing system focusing at the moment;
s4, capturing and operating the optical tweezers: the laser (503) is turned on, the position of a light spot where laser is converged is found in a sample image, the X-axis knob and the Y-axis knob of the three-dimensional fine adjustment displacement table (302) are moved to the vicinity of particles needing to be controlled, the particles under the action of light pressure can be pushed to the light spot and are restrained at the optical trap, and at the moment, the restrained particles can be subjected to displacement control, screening, spectrum research and the like.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311854171.6A CN117572623A (en) | 2023-12-29 | 2023-12-29 | Double-objective integrated optical tweezers system and operation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311854171.6A CN117572623A (en) | 2023-12-29 | 2023-12-29 | Double-objective integrated optical tweezers system and operation method thereof |
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| CN117572623A true CN117572623A (en) | 2024-02-20 |
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| CN202311854171.6A Pending CN117572623A (en) | 2023-12-29 | 2023-12-29 | Double-objective integrated optical tweezers system and operation method thereof |
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- 2023-12-29 CN CN202311854171.6A patent/CN117572623A/en active Pending
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