CN117517278A

CN117517278A - Immunofluorescence detection device

Info

Publication number: CN117517278A
Application number: CN202311510345.7A
Authority: CN
Inventors: 惠开进; 刘原; 仲苏明
Original assignee: Suzhou Tumo Medical Technology Co ltd
Current assignee: Suzhou Tumo Medical Technology Co ltd
Priority date: 2023-11-14
Filing date: 2023-11-14
Publication date: 2024-02-06

Abstract

The invention discloses an immunofluorescence detection device, comprising: the camera comprises an upper supporting beam, a lower supporting beam, a left supporting beam, a right supporting beam, a bottom plate, a camera, a tube lens, an XY moving platform, an objective, a fluorescent light source, an objective table, a PC (personal computer) and a control card, wherein the XY moving platform is fixedly arranged on the upper supporting beam, the tube lens is fixedly arranged above the XY moving platform, and the camera is arranged above the tube lens; the PC is fixedly arranged on the left side of the upper supporting beam, the control card is fixedly arranged on the right side of the upper supporting beam, the objective lens moving table is fixedly arranged in the middle of the upper supporting beam, the objective lens moving table extends to the lower side of the lower supporting beam, and the objective lens is arranged below the objective lens moving table; the fluorescent light source is fixedly arranged on the lower supporting beam; the object stage is placed on the bottom plate; the control card is connected with the XY moving platform and the objective moving platform through cables.

Description

Immunofluorescence detection device

Technical Field

The invention relates to the technical field of medical detection instruments, in particular to an immunofluorescence detection device.

Background

Immunofluorescence technology, also known as fluorescent antibody technology, is the earliest one of the label immunotechniques. The basic reaction of immunology is an antigen-antibody reaction. Since the antigen-antibody reaction has high specificity, when the antigen-antibody reaction occurs, one factor can be found out as long as the other factor is known. Immunofluorescence is a technique in which fluorescent pigments which do not affect the activity of antigen-antibody are marked on the antibody (or antigen), and after being combined with the corresponding antigen (or antibody), a specific fluorescent reaction is presented under a fluorescent microscope.

Most of the existing fluorescence microscopes use a microscope with twenty-to forty-fold magnification, the depth of field of the microscope with twenty-fold magnification is generally between 1 and 1.4 microns according to different brands, and the volumes of fungi, hyphae and bacteria are different from 0.5um to 20um due to the fact that samples are cells with different sizes and heights. If the real-time focusing is not carried out, a large number of acquired pictures are blurred and can not be used for pathological diagnosis.

Digital microscopes based on traditional microscope transformation often install the laser focusing system that the cost is high, cause the cost too high. The digital biological microscope can only scan out the pictures, and needs to occupy a great deal of time for doctors to view the pictures.

Disclosure of Invention

The present invention is directed to an immunofluorescence detection apparatus that overcomes the above-mentioned drawbacks of the prior art.

To achieve the above object, there is provided an immunofluorescence detection apparatus comprising: the device comprises a bottom plate, a left supporting beam, a right supporting beam, an upper supporting beam, a lower supporting beam, an XY moving platform, a tube mirror, a camera, a PC, a control card, an objective moving table, an objective, a fluorescent light source and an objective table, wherein the left supporting beam and the right supporting beam are fixedly arranged on the left side and the right side of the bottom plate, the upper supporting beam is fixedly arranged above the left supporting beam and the right supporting beam, and the lower supporting beam is fixedly arranged in the middle of the left supporting beam and the right supporting beam;

the XY moving platform is fixedly arranged on the upper supporting beam, the tube mirror is fixedly arranged above the XY moving platform, and the camera is arranged above the tube mirror; the PC is fixedly arranged on the left side of the upper supporting beam, the control card is fixedly arranged on the right side of the upper supporting beam, the objective lens moving table is fixedly arranged in the middle of the upper supporting beam, the objective lens moving table extends to the lower side of the lower supporting beam, and the objective lens is arranged below the objective lens moving table; the fluorescent light source is fixedly arranged on the lower supporting beam; the object stage is placed on the bottom plate; the control card is connected with the XY moving platform and the objective moving platform through cables.

Further, the imaging pixel of the camera is more than or equal to 2000 ten thousand pixels, and the tube mirror is a 0.5x tube mirror.

Further, the fluorescent light source is located at the rear side of the objective lens moving table, the power of the fluorescent light source is 10W, and a circular fin type thermal module with the diameter of 6cm and the length of 8cm is further arranged on the fluorescent light source.

Further, a light source switching motor is further arranged on the lower supporting beam, the light source switching motor is arranged on the left side of the fluorescent light source, a light source positioning sensor is fixed on the rear side of the light source switching motor, and the fluorescent light source, the light source switching motor and the light source positioning sensor are connected with the control card through cables.

Further, the stage includes: base, two-dimensional mobile platform and piece clamp, fixed mounting on the base two-dimensional mobile platform sets up the piece on the two-dimensional mobile platform and presss from both sides the recess, piece presss from both sides the recess bottom and is equipped with electro-magnet and piece clamp sensor, the piece press from both sides install in the piece presss from both sides the recess, piece presss from both sides the bottom and is equipped with magnet.

Further, the two sides of the sheet clamp are provided with concave side grooves, protrusions corresponding to the concave side grooves on the two sides are arranged in the sheet clamp grooves, and a bayonet and a glass slide groove are further arranged above the sheet clamp.

Further, the two-dimensional moving platform includes: the X platform and the Y platform are orthogonally stacked on the base, and the X platform is arranged above the Y platform;

the rotor of the X-axis driving motor is arranged on the X platform, the stator of the X-axis driving motor is arranged on the Y platform, the X-axis sensor is fixedly arranged on the rear side of the X-axis driving motor, the X-axis crossed roller guide rail is arranged on the X platform, the X-axis driving motor is connected with the X-axis crossed roller guide rail through a cable, and the X-axis sensor is respectively connected with the X-axis driving motor and the control card through cables;

the rotor of the Y-axis driving motor is arranged on the Y platform, the stator of the Y-axis driving motor is arranged on the X platform, the Y-axis sensor is fixedly arranged on the right side of the Y-axis driving motor, the Y-axis crossed roller guide rail is arranged below the Y platform, the Y-axis driving motor is connected with the Y-axis crossed roller guide rail through a cable, and the Y-axis sensor is respectively connected with the Y-axis driving motor and the control card through cables.

Further, the PC is provided with a gynecological leucorrhea fluorescence scanning method, and the gynecological leucorrhea fluorescence scanning method comprises the following specific steps:

s01: dividing a sample to be scanned into a plurality of areas with equal areas, and scanning the sample from inside to outside in a spiral line mode;

s02: adopting a progressive focusing method in each region, namely adopting images at 200um intervals, and searching the approximate position of the cells; picking up images at intervals of 10um, and searching for the accurate position of the cells; cell images;

s03: gradually diffusing outwards according to a spiral line mode, and collecting a whole rectangular area;

s04: the pictures are spliced into a complete picture in sequence, and pathogenic bacteria and pathological cells are automatically identified and marked on the picture through a deep learning means;

s05: and counting the number of pathogenic bacteria/pathological cells, and generating a report.

Compared with the prior art, the invention has the beneficial effects that:

1. the optical imaging mechanism is combined with the low-magnification optical imaging system, so that a longer depth of field can be obtained;

2. a high-resolution camera is used for obtaining pictures with higher pixel density, and the whole equivalent system has high magnification;

3. the high-brightness fluorescent light source is used, so that the exposure time of a camera is reduced, the photographing speed is improved, and the equipment cost is reduced;

4. the focusing algorithm with focus prediction is configured, so that the number of pictures required for focusing is reduced, and the detection speed is improved;

5. the operation flow is simple and convenient, the system can automatically focus only by putting the manufactured sample into the sheet clamp to be placed on the objective table, the collection does not need manual intervention, the use threshold is greatly reduced, and the labor cost is reduced.

Drawings

FIG. 1 is a schematic elevational view of the structure of the present invention;

FIG. 2 is a schematic rear view of the structure of the present invention;

FIG. 3 is a schematic view of a stage structure according to the present invention;

FIG. 4 is a schematic view of a magnetic attraction structure for a structural sheet according to the present invention

FIG. 5 is a schematic view of a vertical cross-section of a stage according to the present invention;

FIG. 6 is a schematic cross-sectional view of a stage according to the present invention.

Reference numerals in the drawings: 1. a bottom plate; 2. a left support beam; 3. a right support beam; 4. an upper support beam; 5. a lower support beam; 6. an XY moving platform; 7. a tube mirror; 8. a camera; 9. a PC; 10. a control card; 11. an objective lens moving stage; 12. an objective lens; 13. a fluorescent light source; 14. a light source switching motor; 15. a light source positioning sensor; 16. a cable; 17. an objective table; 18. a base; 19. a two-dimensional moving platform; 20. a sheet clamp; 21. a clip groove; 22. a magnet; 23. a wafer chuck sensor; 24. a side groove; 25. a protrusion; 26. a bayonet; 27. a slide glass groove; 28. an X platform; 29. x-axis crossed roller guide rails; 30. an X-axis driving motor; 31. an X-axis sensor; 32. a Y platform; 33. y-axis crossed roller guide rails; 34. a Y-axis driving motor; 35. a Y-axis sensor; 36. an electromagnet.

Detailed Description

The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.

Referring to fig. 1-2, the present invention provides an immunofluorescence detection apparatus, comprising: the device comprises a bottom plate 1, a left supporting beam 2, a right supporting beam 3, an upper supporting beam 4, a lower supporting beam 5, an XY moving platform 6, a tube mirror 7, a camera 8, a PC 9, a control card 10, an objective moving table 11, an objective 12, a fluorescent light source 13 and an objective table 17, wherein the left supporting beam 2 and the right supporting beam 3 are fixedly arranged on the left side and the right side of the bottom plate 1, the upper supporting beam 4 is fixedly arranged above the left supporting beam 2 and the right supporting beam 3, and the lower supporting beam 5 is fixedly arranged in the middle of the left supporting beam 2 and the right supporting beam 3.

The XY moving platform 6 is fixedly mounted on the upper supporting beam 4, and is used for adjusting the positions of the tube mirror 7, the fluorescent light source 13 and the objective lens 12 to align the tube mirror with the fluorescent light source 13. The tube mirror 7 is fixedly arranged above the XY moving platform 6, the tube mirror 7 is a 0.5x tube mirror 7, and the optical magnification is reduced to obtain a longer depth of field, and the optical magnification is 15-20 x. The camera 8 is arranged above the tube mirror 7, and the imaging pixels of the camera 8 are 3000 ten thousand pixels, so that higher pixel density is obtained.

The PC 9 is fixedly arranged on the left side of the upper supporting beam 4, and the PC 9 is provided with a gynecological leucorrhea fluorescence scanning method, and the gynecological leucorrhea fluorescence scanning method comprises the following specific steps:

The control card 10 is fixedly installed on the right side of the upper supporting beam 4, and the control card 10 is connected with the XY moving platform 6 and the objective moving platform 11 through a cable 16 and used for controlling the platform to move. The objective lens moving table 11 is fixedly installed in the middle of the upper supporting beam 4, the objective lens moving table 11 extends to the lower side of the lower supporting beam 5, the objective lens 12 is installed below the objective lens moving table 11, and the position of the objective lens 12 is adjusted by adjusting the objective lens moving table 11.

The fluorescent light source 13 is fixedly installed on the lower supporting beam 5, the fluorescent light source 13 is located at the rear side of the objective lens moving table 11, the power of the fluorescent light source 13 is 10W, a round fin type thermal module with the diameter of 6cm and the length of 8cm is further arranged on the fluorescent light source 13, a light source switching motor 14 is arranged on the lower supporting beam 5, the light source switching motor 14 is arranged at the left side of the fluorescent light source 13, a light source positioning sensor 15 is fixed at the rear side of the light source switching motor and used for adjusting the position of the fluorescent light source 13, and the fluorescent light source 13, the light source switching motor 14 and the light source positioning sensor 15 are connected with the control card 10 through a cable 16.

The stage 17 is placed on the base plate 1, and as shown in fig. 3 to 4, the stage 17 includes: the two-dimensional mobile platform 19 is fixedly mounted on the base 18, the two-dimensional mobile platform 19 is provided with a sheet clamp groove 21, an electromagnet 36 and a sheet clamp sensor 23 are arranged at the bottom of the sheet clamp groove 21, the sheet clamp 20 is mounted in the sheet clamp groove 21, a magnet 22 is arranged at the bottom of the sheet clamp 20, concave side grooves 24 are formed in two sides of the sheet clamp 20, protrusions 25 corresponding to the concave side grooves 24 in two sides are arranged in the sheet clamp groove 21, a bayonet 26 and a glass slide groove 27 are further formed in the upper side of the sheet clamp 20, and after the sheet clamp sensor 23 detects that the sheet clamp 20 is inserted, the electromagnet 22 works to push the sheet clamp 20 upwards, so that the protrusions 25 of the mobile platform clamp the grooves of the sheet clamp 20.

As shown in fig. 5 to 6, the two-dimensional moving platform 19 includes: an X-stage 28, an X-axis cross roller rail 29, an X-axis drive motor 30, an X-axis sensor 31, a Y-stage 32, a Y-axis cross roller rail 33, a Y-axis drive motor 34, and a Y-axis sensor 35, wherein the X-stage 28 and the Y-stage 32 are stacked on the base 18 in an orthogonal manner, and the X-stage 28 is disposed above the Y-stage 32; the X-axis driving motor 30 is disposed at the rear side of the X-axis platform 28, the X-axis sensor 31 is fixedly mounted at the rear side of the X-axis driving motor 30, the rotor of the X-axis driving motor 30 is disposed on the X-axis platform 28, the stator of the X-axis driving motor 30 is disposed on the Y-axis platform 32, the X-axis sensor 31 is fixedly mounted at the rear side of the X-axis driving motor 30, the X-axis cross roller guide 29 is disposed on the X-axis platform 28, the X-axis driving motor 30 is connected with the X-axis cross roller guide 29 through a cable 16, and the X-axis sensor 31 is respectively connected with the X-axis driving motor 30 and the control card 10 through the cable 16;

the rotor of the Y-axis driving motor 34 is arranged on the Y-platform 32, the stator of the Y-axis driving motor 34 is arranged on the X-platform 28, the Y-axis sensor 35 is fixedly arranged on the right side of the Y-axis driving motor 3, the Y-axis crossed roller guide rail 33 is arranged below the Y-platform 32, the Y-axis driving motor 34 is connected with the Y-axis crossed roller guide rail 33 through a cable 16, and the Y-axis sensor 35 is respectively connected with the Y-axis driving motor 34 and the control card 10 through the cable 16. The moving part of the two-dimensional moving platform 19 is made of 2 moving modules which are orthogonally placed, each moving module is connected by 2 pairs of high-precision guide rails, and is driven by a linear motor, the X-axis sensor 31 and the Y-axis sensor 35 feed back position information in real time, so that the moving precision of 0.1um can be realized.

Working principle: after the equipment is started, the slide glass is clamped on the clamp 20, the clamp 20 is inserted on the upper objective table 17, after the clamp sensor 23 detects that the clamp 20 is inserted, the electromagnet 22 works to push the clamp 20 upwards, so that the protrusion 25 of the movable platform clamps the groove of the clamp 20; the PC 9 sends instructions to the control card 10, the control card 10 controls the XY moving platform object 6 to move according to the signals sent by the light source positioning sensor 15, meanwhile controls the objective moving platform 11 to move, and is used for adjusting the positions of the tube mirror 7, the fluorescent light source 13 and the objective 12 to align the two-dimensional moving platform 19, the control card 10 controls the two-dimensional moving platform 19 to work, so that the glass slide is positioned below the objective 12, the PC 9 controls the camera 8 to shoot a picture, meanwhile sends instructions to the control card 10, controls the objective moving platform 11 to move up and down, the PC selects the optimal position according to the definition of the picture through pictures shot by CCD, controls the objective moving platform 11, the XY moving platform object 6 and the two-dimensional moving platform 19 to move and controls the camera 8 to shoot repeatedly until the pictures at all positions are shot clearly, namely the whole glass slide scanning is completed

Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. An immunofluorescence detection apparatus comprising: bottom plate (1), left support crossbeam (2), right support crossbeam (3), go up support crossbeam (4), lower support crossbeam (5), XY moving platform (6), tube mirror (7), camera (8), PC (9), control card (10), objective mobile station (11), objective (12), fluorescent light source (13) and objective table (17), its characterized in that:

the left support beam (2) and the right support beam (3) are fixedly arranged on the left side and the right side of the bottom plate (1), the upper support beam (4) is fixedly arranged above the left support beam (2) and the right support beam (3), and the lower support beam (5) is fixedly arranged in the middle of the left support beam (2) and the right support beam (3);

the XY moving platform (6) is fixedly arranged on the upper supporting beam (4), the tube mirror (7) is fixedly arranged above the XY moving platform (6), and the camera (8) is arranged above the tube mirror (7); the PC (9) is fixedly arranged on the left side of the upper supporting beam (4), the control card (10) is fixedly arranged on the right side of the upper supporting beam (4), the objective lens moving table (11) is fixedly arranged in the middle of the upper supporting beam (4), the objective lens moving table (11) extends to the lower side of the lower supporting beam (5), and the objective lens (12) is arranged below the objective lens moving table (11); the fluorescent light source (13) is fixedly arranged on the lower supporting beam (5); the object stage (17) is placed on the bottom plate (1); the control card (10) is connected with the XY moving platform (6) and the objective moving platform (11) through a cable (16).

2. An immunofluorescence detection apparatus according to claim 1, wherein: the imaging pixels of the camera (8) are more than or equal to 2000 ten thousand pixels, and the tube mirror (7) is a 0.5x tube mirror (7).

3. An immunofluorescence detection apparatus according to claim 1, wherein: the fluorescent light source (13) is positioned at the rear side of the objective lens moving table (11), the power of the fluorescent light source (13) is 10W, and the fluorescent light source (13) is also provided with a circular fin type thermal module with the diameter of 6cm and the length of 8 cm.

4. An immunofluorescence detection apparatus according to claim 1, wherein: the lower support beam (5) on still be provided with light source switch motor (14), light source switch motor (14) set up in the left side of fluorescence light source (13), a light source positioning sensor (15) is fixed to the rear side of light source switch motor (14), fluorescence light source (13), light source switch motor (14) and light source positioning sensor (15) pass through cable (16) with control card (10) are connected.

5. An immunofluorescence detection apparatus according to claim 1, wherein: the stage (17) includes: base (18), two-dimensional mobile platform (19) and piece clamp (20), fixed mounting on base (18) two-dimensional mobile platform (19), set up piece clamp groove (21) on two-dimensional mobile platform (19), piece clamp groove (21) bottom is equipped with electro-magnet (36) and piece clamp sensor (23), piece clamp (20) install in piece clamp groove (21), piece clamp (20) bottom is equipped with magnet (22).

6. An immunofluorescence detection apparatus according to claim 5, wherein: the sheet clamp is characterized in that concave side grooves (24) are formed in two sides of the sheet clamp (20), protrusions (25) corresponding to the concave side grooves (24) in two sides are arranged in the sheet clamp grooves (21), and a bayonet (26) and a glass slide groove (27) are further formed in the upper portion of the sheet clamp (20).

7. An immunofluorescence detection apparatus according to claim 5, wherein: the two-dimensional moving platform (19) comprises: an X platform (28), an X-axis crossed roller guide rail (29), an X-axis driving motor (30), an X-axis sensor (31), a Y platform (32), a Y-axis crossed roller guide rail (33), a Y-axis driving motor (34) and a Y-axis sensor (35), wherein the X platform (28) and the Y platform (32) are orthogonally stacked on the base (18), and the X platform (28) is arranged above the Y platform (32);

the rotor of the X-axis driving motor (30) is arranged on the X-axis platform (28), the stator of the X-axis driving motor (30) is arranged on the Y-axis platform (32), the X-axis sensor (31) is fixedly arranged on the rear side of the X-axis driving motor (30), the X-axis crossed roller guide rail (29) is arranged on the X-axis platform (28), the X-axis driving motor (30) and the X-axis crossed roller guide rail (29) are connected through a cable (16), and the X-axis sensor (31) is respectively connected with the X-axis driving motor (30) and the control card (10) through the cable (16);

the rotor of Y axle driving motor (34) set up in on Y platform (32), the stator of Y axle driving motor (34) set up in on X platform (28), Y axle sensor (35) fixed mounting in Y axle driving motor (34) right side, Y axle cross roller guide (33) set up in Y platform (32) below, Y axle driving motor (34) with Y axle cross roller guide (33) are connected through cable (16), Y axle sensor (35) pass through cable (16) respectively with Y axle driving motor (34) and control card (10) are connected.

8. An immunofluorescence detection apparatus according to claim 1, wherein: the PC (9) is provided with a gynecological leucorrhea fluorescence scanning method, and the gynecological leucorrhea fluorescence scanning method comprises the following specific steps: