CN117502243A - Tissue culture seedling inoculation device and inoculation method - Google Patents

Abstract

The invention discloses a tissue culture seedling inoculation device, which comprises a rack; a rotating part arranged on the frame; the seedling placing station is arranged on the rotating part and can drive the seedling placing station to rotate around the vertical axis of the rotating part, and the seedling placing station is used for placing tissue culture seedlings; two ends of the seedling cutting stations are arranged on the frame and are positioned at the outer side of the rotating part, and can cut stem leaves of tissue culture Miao Duanbu and roots; the middle seedling separating station is arranged on the frame and positioned at the outer side of the rotating part, and can cut along the stem direction of the tissue culture seedling to divide the tissue culture seedling into two parts; the seedling taking, transplanting and inoculating station is arranged on the frame and positioned at the outer side of the rotating part, can clamp the tissue culture seedlings cut on the seedling placing station through clamping jaws, and is transplanted into the tissue culture bottle to finish inoculation. The invention also provides a tissue culture seedling inoculation method based on the device, and the machine replaces manual seedling cutting inoculation, so that the manual operation intensity is reduced, and the working efficiency is improved.

Description

Tissue culture seedling inoculation device and inoculation method

Technical Field

The invention relates to the technical field of tissue culture seedling inoculation, in particular to a tissue culture seedling inoculation device and an inoculation method.

Background

The plant tissue culture technology is a rapid and effective way for obtaining a large number of healthy high-quality seedlings in a short time. With the increase of farm product demands, the agricultural crop planting area is continuously enlarged, and the demand of high-quality seedlings is continuously increased. At present, manual operation is generally adopted in the inoculation link of the tissue culture seedling, and operators use tools such as tweezers and scissors after disinfection to complete tissue culture Miao Shequ, segmentation and inoculation operation, so that when the tissue culture seedling is produced on a large scale, the inoculation quantity is large, a large amount of manpower and production data are required to be consumed, and the production efficiency is difficult to meet the requirement of mass production.

Disclosure of Invention

The invention aims to provide a tissue culture seedling inoculation device and an inoculation method, which are used for solving the problems in the prior art, and the mechanical automatic inoculation of the tissue culture seedlings is adopted, so that the labor cost is reduced, and the production efficiency is improved.

In order to achieve the above object, the present invention provides the following solutions:

the invention provides a tissue culture seedling inoculation device, which comprises a rack; a rotating part arranged on the frame; the seedling placing station is arranged on the rotating part and can drive the seedling placing station to rotate around the vertical axis of the rotating part, and the seedling placing station is used for placing tissue culture seedlings; two ends of the seedling cutting stations are arranged on the frame and are positioned outside the rotating part, and can cut stem leaves of tissue culture Miao Duanbu and roots; the middle seedling separating station is arranged on the frame and positioned at the outer side of the rotating part, and can cut along the stem direction of the tissue culture seedling to divide the tissue culture seedling into two parts; the seedling taking, transplanting and inoculating station is arranged on the frame and positioned at the outer side of the rotating part, and can clamp the tissue culture seedlings cut on the seedling placing station through clamping jaws and transplant the tissue culture seedlings into the tissue culture bottles to finish inoculation.

Optionally, the stand comprises a supporting frame, a mounting plate is fixedly arranged at the top of the supporting frame, and the rotating part, the seedling cutting stations at two ends, the middle seedling separating station and the seedling taking and transplanting inoculation station are respectively arranged on the mounting plate; the bottom of the supporting frame is provided with a plurality of rollers.

Optionally, the seedling transplanting and inoculating device further comprises a finished product material frame which is arranged on the frame and positioned obliquely below one end of the seedling transplanting and inoculating station and used for placing the tissue culture bottle after the tissue culture seedling is inoculated.

Optionally, the gyration portion including set up in cam divider in the frame, cam divider top is equipped with the mount table, the mount table is last evenly to be provided with a plurality of put seedling station.

Optionally, the seedling placing station comprises a seedling placing tool fixedly arranged on the rotating part, a trapezoid opening is formed in the top of the seedling placing tool, a blade avoiding groove is formed in the bottom of the trapezoid opening, and the blade avoiding groove is used for placing tissue culture seedlings; the seedling placing tool is provided with a seedling clamping plate avoiding groove and a transplanting clamping jaw avoiding groove which are arranged in parallel, and the clamp Miao Ban avoiding groove and the transplanting clamping jaw avoiding groove are both vertically arranged with the blade avoiding groove; the seedling placing tool is characterized in that seedling clamping plates are symmetrically arranged on two sides of the seedling placing tool, an opening is formed in one end of each seedling clamping plate, a first connecting plate is connected to the other end of each seedling clamping plate through a first spring, a clamping cylinder is connected to the first connecting plate, when the clamping cylinder contracts, the seedling clamping plates can be driven to be inserted into the clamp Miao Ban to avoid centering clamping of tissue culture seedlings in the groove, elastic force can be provided by the first springs in the movement process of the seedling clamping plates, the buffering effect is achieved, then the seedling clamping plates are driven by the clamping cylinders to clamp the tissue culture seedlings Miao Shi through the action of the first springs, the clamping force of the seedling clamping plates is more flexible, the seedling clamping plates can adapt to the tissue culture seedlings clamping different stems, and the situation that the tissue culture seedlings are clamped off is avoided.

Optionally, the seedling cutting stations at two ends comprise a supporting plate fixedly arranged on the frame, a locking cylinder is connected to the supporting plate through a locking cylinder mounting plate, a second connecting plate is fixedly arranged at the tail end of a cylinder rod of the locking cylinder, a first pressing block is installed on the second connecting plate, the first pressing block is integrally trapezoid and can be matched with a trapezoid opening of the seedling placing tool, an open slot is formed in the first pressing block, and the vertical projection of the open slot coincides with the vertical projection of the clamp Miao Ban avoiding slot; the front end and the rear end of the first pressing block are symmetrically provided with scissor cylinders, the bottoms of cylinder rods of the scissor cylinders are connected with scissors, and the scissor cylinders can drive the scissors to be closed to finish shearing actions of root parts and stem rod end parts of tissue culture seedlings.

Optionally, the two-end seedling cutting station further comprises a waste collection mechanism; the waste collection mechanism comprises a waste collection frame and a guide wheel plate arranged on the frame, wherein a guide wheel is movably arranged on the guide wheel plate, and the guide wheel is arranged at the bottom of the waste collection frame and can drive the waste collection frame to move to the position below the seedling placing stations at the two ends of the seedling cutting stations; the end part of the waste material collecting frame is fixedly connected with a handle.

Optionally, the middle section seedling separating station comprises a support frame fixedly arranged on the frame, a cutting Miao Qigang is arranged on one side of the top of the support frame, a pushing plate is connected to a cylinder rod at the bottom of the cutting Miao Qigang, a plurality of guide shafts are fixedly connected to the bottom of the pushing plate, a second pressing block is arranged below the pushing plate, a plurality of guide bushings are arranged on the second pressing block, the guide shafts penetrate through the guide bushings, the size of a limiting part at the bottom of the guide shaft is larger than the inner diameter of the guide bushings, a blade is fixedly arranged at the bottom of the pushing plate, and the blade penetrates through a strip-shaped hole formed in the second pressing block; the guide shaft is sleeved with a second spring, and the second spring is positioned between the push plate and the second pressing block; the second pressing block is integrally trapezoidal and can be matched with a trapezoid opening of the seedling placing tool, a second pressing block clamp Miao Ban avoiding groove and a second pressing block blade avoiding groove are formed in the second pressing block, the second pressing block clamp Miao Ban avoiding groove is correspondingly arranged with the clamp Miao Ban avoiding groove, and the second pressing block blade avoiding groove is correspondingly arranged with the blade avoiding groove; the second pressing block is provided with a guide bushing mounting hole, and the guide bushing is fixedly arranged in the guide bushing mounting hole.

Optionally, the seedling taking, transplanting and inoculating station comprises a seedling taking, transplanting and inoculating frame fixedly arranged on the frame, a traversing mechanism is arranged at the top of the seedling taking, transplanting and inoculating frame, a clamping mechanism is arranged at the inner side of the traversing mechanism, and the traversing mechanism can drive the clamping mechanism to traverse; an inoculation station is arranged on the inner side of the seedling taking, transplanting and inoculating machine frame, the inoculation station comprises a bottle rack fixedly arranged on the machine frame, and a tissue culture bottle is placed on the bottle rack; the clamping mechanism comprises a mounting bottom plate vertically arranged on the transverse moving mechanism, a guide rail is arranged on one side of the mounting bottom plate, a clamping jaw cylinder mounting plate is arranged on the guide rail in a sliding mode, one end of the clamping jaw cylinder mounting plate is connected with a driving cylinder, a clamping jaw cylinder is arranged on the clamping jaw cylinder mounting plate, clamping jaws are arranged at the bottoms of the clamping jaw cylinders, the clamping jaws can be driven to open or close by the clamping jaw cylinders, and clamping jaw pads are arranged on the inner sides of the clamping jaws.

The invention also provides a tissue culture seedling inoculation method based on the device, which comprises the following steps:

step one, taking out tissue culture seedlings from a tissue culture container, placing the tissue culture seedlings in a seedling placing tool in a seedling placing station at the first station, controlling a clamping cylinder to extend out, driving a seedling clamping plate on the clamping cylinder to move in a centering manner, and placing the tissue culture Miao Gachi in a seedling clamping area;

step two, after the tissue culture seedling is clamped, driving the cam divider to rotate by 90 degrees, at the moment, the tissue culture seedling clamped in the seedling placing station arrives at the seedling cutting stations at the two ends, then driving the locking cylinder to retract, driving the first pressing block to downwards compress on the seedling placing tool to clamp the tissue culture seedling in the vertical direction, simultaneously, driving the scissor cylinder to downwards move right above the root and stem leaf end of the tissue culture seedling after the locking cylinder is retracted, and then driving the scissor cylinder to act, closing the scissors to finish the shearing action of the root and stem end of the tissue culture seedling; simultaneously, the seedling placing tool originally positioned at the seedling taking, transplanting and inoculating station is rotated to the first station, and then the first step is repeated to finish the seedling placing action;

step three, driving the cam divider to rotate 90 degrees again, moving the tissue culture seedlings to a middle seedling dividing station, driving the cutter Miao Qigang to drive the second pressing block to move downwards to press the seedling placing tool so as to clamp the tissue culture seedlings, then continuously moving the pushing plate downwards, driving the blade to continuously move downwards to the blade avoiding groove, and at the moment, finishing stem cutting of the tissue culture seedlings, so that the tissue culture seedlings are divided into two parts; simultaneously, rotating the seedling placing tool for re-completing seedling feeding to the positions of two end seedling cutting stations in the second step, and repeating the first step and the second step to complete the actions of re-feeding and head-to-tail seedling cutting of the tissue culture seedlings;

driving the cam divider to rotate 90 degrees again, and moving the tissue culture seedlings to a seedling taking, transplanting and inoculating station; firstly, driving a transverse moving mechanism to act, driving clamping jaws in a clamping mechanism to move to a seedling grabbing position above tissue culture Miao Zheng, then driving an air cylinder to move downwards to drive the clamping jaws to move downwards to a seedling placing tool transplanting clamping jaw avoiding groove, and driving the clamping jaws to retract to drive the clamping jaws to close so as to realize tissue culture seedling clamping action; the clamping cylinder is opened, the seedling clamping plates move to two sides, and the seedling opening tool is opened to clamp the tissue culture seedlings; then driving the air cylinder to move upwards to drive the clamping jaw to move upwards to separate from the seedling placing tool; the transverse moving mechanism drives the clamping jaw to move leftwards to be right above the inoculation station, and drives the air cylinder to move downwards to drive the tissue culture seedling to move to a position 3mm above the opening of the tissue culture bottle; then the clamping jaw cylinder is opened to drive the clamping jaw to open, and the tissue culture seedling falls into the tissue culture bottle under the action of gravity to finish the inoculation action; repeating the first, second and third steps, and repeating the actions of seedling feeding, head-to-tail seedling cutting and seedling dividing;

step five, driving the cam divider to rotate 90 degrees again after the tissue culture seedlings are inoculated, and returning the seedling placing station to the first station; and then repeating the first step to the fourth step to continuously perform the seedling separating inoculation action of the tissue culture seedlings.

Compared with the prior art, the invention has the following technical effects:

according to the invention, the machine replaces manual seedling cutting and inoculation, so that the manual operation intensity is reduced, and the working efficiency is improved; the invention cuts Miao Shi with higher precision, ensures the consistency of tissue culture Miao Changdu after cutting seedlings, has flat cut surfaces at two ends and improves the survival rate of subsequent inoculation.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are needed in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.

FIG. 1 is a schematic diagram of a tissue culture seedling inoculating device;

FIG. 2 is a schematic view of a seedling laying station according to the present invention;

FIG. 3 shows the seedling placing station of the invention in an opened state before seedling placing;

FIG. 4 shows the clamping state of the seedling placing station after seedling placement;

FIG. 5 is another angular schematic view of the seedling laying station of the present invention;

FIG. 6 is a schematic diagram of a two-end seedling cutting station structure of the invention;

FIG. 7 is a schematic view of a first briquette structure according to the present invention;

FIG. 8 is a schematic diagram of a seedling placing tool according to the invention;

FIG. 9 is a schematic view of a waste collection mechanism of the present invention;

FIG. 10 is a schematic view of a middle section seedling separation station according to the present invention;

FIG. 11 is a schematic view of a second briquette structure according to the present invention;

FIG. 12 is a schematic view of a seedling taking, transplanting and inoculating station according to the present invention;

FIG. 13 is a schematic view of a traversing mechanism according to the present invention;

FIG. 14 is a schematic view of the structure of the clamping jaw of the present invention;

FIG. 15 is a schematic view of an inoculating station of the present invention;

FIG. 16 is a schematic flow chart of the inoculation method of the present invention.

Reference numerals illustrate: 1-seedling placing station, 101-clamping cylinder, 102-first connecting plate, 103-first spring, 104-seedling clamping plate, 105-seedling placing Miao Gongzhuang, 1051-blade avoiding groove, 1052-clamp Miao Ban avoiding groove, 1053-transplanting clamping jaw avoiding groove, 2-two-end seedling cutting station, 201-supporting plate, 202-locking cylinder mounting plate, 203-locking cylinder, 204-second connecting plate, 205-scissor cylinder, 206-first pressing block, 2061-opening groove, 207-waste collecting mechanism, 2071-handle, 2072-guide wheel, 2073-guide wheel plate, 2074-waste collecting frame, 3-middle seedling dividing station, 301-supporting frame, 302-cutting Miao Qigang, 303-pushing plate, 304-guide bush, 305-second pressing block, cutting Miao Qigang 3051-second briquetting clamp Miao Ban avoidance groove, 3052-second briquetting blade avoidance groove, 3053-guide bushing mounting hole, 306-second spring, 307-guide shaft, 308-blade, 4-seedling taking, transplanting and inoculating station, 41-seedling taking, transplanting and inoculating frame, 42-traversing mechanism, 421-rodless cylinder, 422-linear guide rail, 423-third connecting plate, 43-clamping mechanism, 431-mounting plate, 432-guide rail, 433-driving cylinder, 434-clamping jaw cylinder mounting plate, 435-clamping jaw cylinder, 436-clamping jaw, 437-clamping jaw pad, 44-inoculating station, 441-bottle holder, 442-tissue culture bottle, 5-frame, 6-finished material frame, 7-cam divider, 8-control switch.

Detailed Description

The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.

The invention aims to provide a tissue culture seedling inoculation device and an inoculation method, which are used for solving the problems in the prior art, and the mechanical automatic inoculation of the tissue culture seedlings is adopted, so that the labor cost is reduced, and the production efficiency is improved.

In order that the above-recited objects, features and advantages of the present invention will become more readily apparent, a more particular description of the invention will be rendered by reference to the appended drawings and appended detailed description.

Most of the tissue culture propagation production operations are manually operated, so that the working efficiency is low, a large amount of manpower is required to be consumed, and the practicability is low. In addition, the operations such as tissue culture seedling segmentation, inoculation (seedling planting) and the like are required to be carried out by the culture professional. And the manual operation is Miao Shi, the operation quality is unstable, and the production efficiency is difficult to meet the requirement of mass production.

Aiming at the defects of the prior art, the invention provides a tissue culture Miao Qiemiao inoculation device which solves the problem of low working efficiency of manually operated seedling cutting and inoculation in the prior art. And the consistency of the cut seedling and the incision is ensured by a mechanical seedling cutting mode. As shown in fig. 1 to 15, the structure of the invention comprises a frame 5; the frame 5 is provided with a rotating part, the rotating part is fixedly provided with a seedling placing station 1, the rotating part can drive the seedling placing station 1 to rotate around the vertical axis of the rotating part, and the seedling placing station 1 is used for placing tissue culture seedlings so as to realize the rotation of the tissue culture seedlings; the seedling cutting stations 2 at two ends are arranged on the frame 5 and are positioned at the outer side of the rotating part, and can cut the tissue culture Miao Duanbu and the stem and leaf of the root which rotate to the position; the middle seedling separating station 3 is arranged on the frame 5 and is positioned at the outer side of the rotating part, and can cut the tissue culture seedlings rotating to the position along the stem direction and divide the tissue culture seedlings into two parts; the seedling taking, transplanting and inoculating station 4 is arranged on the frame 5 and is positioned on the outer side of the rotating part, and can clamp the tissue culture seedlings cut on the seedling placing station 1 through the clamping jaw 436 and transplant the tissue culture seedlings into the tissue culture bottle 442 to finish inoculation. Mechanical seedling separation and inoculation are adopted, so that the working efficiency is improved.

In order to further improve the working efficiency and achieve continuous operation on the basis of the above, the invention designs the frame 5 to comprise a supporting frame, and a plurality of rollers are arranged at the bottom of the supporting frame so as to facilitate movement; the support frame top is fixedly provided with a mounting plate, a rotating part, two end seedling cutting stations 2, a middle section seedling dividing station 3 and a seedling taking, transplanting and inoculating station 4 are respectively arranged on the mounting plate at intervals, the position of an initial seedling placing station 1 is defined as a first station, the position of the two end seedling cutting stations 2 is defined as a second station, the position of the middle section seedling dividing station 3 is defined as a third station, the position of the seedling taking, transplanting and inoculating station 4 is defined as a fourth station, four seedling placing stations 1 are respectively arranged on the rotating part at intervals of 90 DEG in a ring, and the positions of the first station to the fourth station correspond to the positions of the first station respectively, so that when the tissue culture seedlings on the seedling placing station 1 on the next station can be continuously placed on the seedling placing station 1, the corresponding operation can be synchronously performed, continuous production can be realized only by operating personnel to control the rotating part to rotate 90 DEG each time through a control switch on the frame 5, and meanwhile, the tissue culture seedlings can be continuously placed on the seedling placing station 1 on the first station, and the efficiency is improved. The finished product material frame 6 is arranged on the frame 5 and is positioned obliquely below one end of the seedling taking, transplanting and inoculating station 4, and is used for placing the tissue culture bottle 442 after the operator finishes inoculating the tissue culture seedlings.

In order to enable the rotation of the seedling placing station 1 to be more accurate and the running to be stable and reliable, the rotating part of the invention adopts the cam divider 7 arranged on the frame 5, the cam divider 7 is electrically connected with the control switch 8, the top of the cam divider 7 is provided with the mounting table, and four seedling placing stations 1 are uniformly arranged on the mounting table, so that the mounting table can rotate 90 degrees each time, and the seedling placing stations 1 can be sequentially moved to the next station. During specific work, the tissue culture seedlings are manually placed on a seedling placing station 1 at the first station, a button of a control switch 8 is pressed, a cam divider 7 is driven to rotate 90 degrees, at the moment, the tissue culture seedlings are moved to two seedling cutting stations 2 at the second station, and the root parts and the end part stems and leaves of the tissue culture seedlings are removed through a scissor cylinder; meanwhile, a second plant of tissue culture seedlings are placed in the seedling placing station 1 which is moved to the station one, and then the button is pressed to drive the tissue culture seedlings to rotate for 90 degrees again. And the tissue culture seedlings after head-tail seedling cutting are moved to a middle seedling separating station 3 of the three stations. The blade is driven to move downwards by the air cylinder, and the tissue culture seedling is divided into two parts along the stem direction of the tissue culture seedling. Then the button is pressed to drive the tissue culture seedling to rotate 90 degrees again, and the tissue culture seedling is moved to the position of the seedling taking and transplanting inoculation station 4 around the station. After the tissue culture seedling is clamped by the clamping jaw 436, the tissue culture seedling is transplanted into a tissue culture bottle, and inoculation is completed. And finally, after the inoculated tissue culture seedlings are provided with bottle caps, placing the tissue culture seedlings into a finished product material frame 6.

As shown in fig. 2, 3, 4, 5, 7 and 8, in order to realize clamping and fixing of the tissue culture seedlings, the seedling placing station 1 of the invention comprises a seedling placing tool 105 fixedly arranged on a rotating part, a trapezoid opening is arranged at the top of the seedling placing tool 105, a blade avoiding groove 1051 is formed at the bottom of the trapezoid opening, and the blade avoiding groove 1051 is used for placing the tissue culture seedlings; the seedling placing tool 105 is provided with a seedling clamping plate avoiding groove 1052 and a transplanting clamping jaw avoiding groove 1053 which are arranged in parallel, and the seedling clamping Miao Ban avoiding groove 1052 and the transplanting clamping jaw avoiding groove 1053 are arranged vertically to the blade avoiding groove 1051; seedling frock 105 bilateral symmetry is equipped with the clamp seedling board 104, and clamp seedling board 104 one end is equipped with the opening, and the other end is connected with first link plate 102 through first spring 103, and first link plate 102 is connected with and presss from both sides tight cylinder 101, and when pressing from both sides tight cylinder 101 shrink, can drive clamp seedling board 104 through first link plate 102 and first spring 103 and insert clamp Miao Ban dodge in the groove 1052 and realize the centering clamp of tissue culture seedling. Before seedling is put, the clamping air cylinder 101 is in an open state, after the tissue culture seedlings are put into the seedling placing tool 105, the control switch is pressed, the clamping air cylinder 101 is closed, and the seedling clamping plates 104 arranged on two sides of the clamping air cylinder 101 are driven to move towards the center, so that the tissue culture seedlings are centered and clamped. In order to adapt to tissue culture seedlings with different diameters, a first spring 103 is arranged between a first connecting plate 102 and a seedling clamping plate 104; when the diameter difference of the tissue culture seedlings is large, when the tissue culture seedlings are clamped by the seedling clamping plate 104 under the driving of the clamping cylinder 101, the tissue culture seedlings push the seedling clamping plate 104 to move to two sides, a proper space is reserved, and the situation that the tissue culture seedlings are clamped off is avoided.

As shown in fig. 6 and 9, in order to be able to trim stem leaves at two ends of the tissue culture Miao Shouwei at the same time, the two-end seedling cutting station 2 comprises a supporting plate 201 fixedly arranged on the frame 5, the supporting plate 201 is connected with a locking cylinder 203 through a locking cylinder mounting plate 202, the tail end of a cylinder rod of the locking cylinder 203 is fixedly provided with a second connecting plate 204, a first pressing block 206 is mounted on the second connecting plate 204, the whole of the first pressing block 206 is trapezoid, and can be matched with a trapezoid opening of the placing Miao Gongzhuang 105, an open slot 2061 is formed in the first pressing block 206, and the vertical projection of the open slot 2061 coincides with the vertical projection of the clamp Miao Ban avoiding slot 1052; the front end and the rear end of the first pressing block 206 are symmetrically provided with scissor cylinders 205, the bottom of a cylinder rod of each scissor cylinder 205 is connected with a pair of scissors, and each scissor cylinder 205 can drive the pair of scissors to be closed, so that shearing actions of the root parts of tissue culture seedlings and the end parts of stem rods are completed. A waste collection mechanism 207 is arranged at the seedling cutting stations 2 at the two ends; the waste collection mechanism 207 comprises a waste collection frame 2074 and a guide wheel plate 2073 arranged on the stand 5, the guide wheel plate 2073 is movably provided with a guide wheel 2072, the guide wheel 2072 is arranged at the bottom of the waste collection frame 2074, and can drive the waste collection frame 2074 to move to the lower part of the seedling placing station 1 at the two-end seedling cutting stations 2; the end part of the waste material receiving frame 2074 is fixedly connected with a handle 2071. When the tissue culture seedling moves to the second station, the locking cylinder 203 is retracted, and the first pressing block 206 is driven to press the seedling placing tool 105, so that tissue culture Miao Gachi is realized. Through handle 2071 control waste material receipts material frame 2074 removal to this station below, then drive scissors cylinder 205 and beat out, the scissors is closed, accomplishes the shearing action of tissue culture seedling root and stem end, and the waste material of shearing falls into waste material receipts material frame 2074 to can the manual work promote waste material receipts material frame 2074 roll-off, the transfer of the waste material of being convenient for.

Before inoculation of the tissue culture seedling, the tissue culture seedling needs to be cut into two parts along the radial direction, as shown in fig. 10 and 11, in order to realize the operation, the middle section seedling separating station 3 of the invention comprises a supporting frame 301 fixedly arranged on a frame 5, one side of the top of the supporting frame 301 is provided with a cutter Miao Qigang, a cylinder rod at the bottom of the cutter Miao Qigang is connected with a push plate 303, the bottom of the push plate 303 is fixedly connected with a plurality of guide shafts 307, a second pressing block 305 is arranged below the push plate 303, the second pressing block 305 is provided with a plurality of guide bushings 304, the guide shafts 307 are arranged in the guide bushings 304 in a penetrating manner, the size of a limiting part at the bottom of the guide shafts 307 is larger than the inner diameter size of the guide bushings 304, the bottom of the push plate 303 is fixedly provided with a blade 308, and the blade 308 is arranged in a strip-shaped hole formed in the second pressing block 305 in a penetrating manner; the guide shaft 307 is sleeved with a second spring 306, and the second spring 306 is positioned between the push plate 303 and the second pressing block 305; the second pressing block 305 is integrally trapezoid and can be matched with a trapezoid opening of the placing Miao Gongzhuang 105, a second pressing block clamp Miao Ban avoiding groove 3051 and a second pressing block blade avoiding groove 3052 are formed in the second pressing block 305, the second pressing block clamp Miao Ban avoiding groove 3051 is correspondingly arranged with the clamp Miao Ban avoiding groove 1052, and the second pressing block blade avoiding groove 3052 is correspondingly arranged with the blade avoiding groove 1051; the second pressing block 305 is provided with a guide bushing mounting hole 3053, and the guide bushing 304 is fixedly mounted in the guide bushing mounting hole 3053. When the tissue culture seedling moves to the station III, the cutter Miao Qigang 302 is driven to punch out, and the second pressing block 305 is driven to move downwards to press the seedling placing tool 105, so that tissue culture Miao Gachi is realized. Then the push plate 303 moves downwards continuously, the blade 308 is driven to move downwards continuously into the blade avoiding groove 1051, and at the moment, the stem cutting of the tissue culture seedling is completed, so that the tissue culture seedling is divided into two parts.

As shown in fig. 12, 13, 14 and 15, the seedling taking, transplanting and inoculating station 4 in the embodiment includes a seedling taking, transplanting and inoculating frame 41 fixedly arranged on a frame 5, a traversing mechanism 42 is arranged at the top of the seedling taking, transplanting and inoculating frame 41, a clamping mechanism 43 is arranged at the inner side of the traversing mechanism 42, the traversing mechanism 42 can drive the clamping mechanism 43 to traverse, the traversing mechanism 42 in the embodiment includes a rodless cylinder 421, a linear guide 422 and a third connecting plate 423 arranged on the linear guide 422, and the rodless cylinder 421 can drive the third connecting plate 423 to traverse on the linear guide 422; the inside of the seedling taking, transplanting and inoculating rack 41 is provided with an inoculating station 44, the inoculating station 44 comprises a bottle rack 441 fixedly arranged on the rack 5, and a tissue culture bottle 442 is placed on the bottle rack 441; the fixture 43 includes the mounting plate 431 of vertical installation on third link 423, mounting plate 431 one side is equipped with guide rail 432, slide on the guide rail 432 and be equipped with clamping jaw cylinder mounting panel 434, clamping jaw cylinder mounting panel 434 one end is connected with actuating cylinder 433, install clamping jaw cylinder 435 on the clamping jaw cylinder mounting panel 434, clamping jaw cylinder 435 bottom is equipped with clamping jaw 436, clamping jaw cylinder 435 can drive clamping jaw 436 and open or close, clamping jaw pad 437 is installed to clamping jaw 436 inboard, clamping jaw pad 437 adopts flexible plastics material such as polyurethane, guarantee when grabbing the seedling, do not destroy the integrality of seedling. The action flow of the seedling taking, transplanting and inoculating station 4 is that after the tissue culture seedlings move to the station four, the clamping cylinder 101 is opened, the seedling clamping plate 104 moves to two sides, and the clamping of the tissue culture seedlings is opened. Then, the traversing mechanism 42 drives the clamping mechanism 43 to move to the seedling picking point. The driving cylinder moves downwards to drive the clamping jaw 436 to move downwards into the transplanting clamping jaw avoiding groove 1053 of the seedling placing tool 105; the clamping jaw cylinder 435 is retracted to drive the clamping jaw 436 to be closed, so that the tissue culture seedling clamping action is realized. Then the cylinder 433 is driven to move upwards, and the clamping jaw 436 is driven to move upwards to be separated from the seedling placing tool 105; the rodless cylinder 421 moves to the left directly above the inoculation station 44. The driving cylinder 433 moves downwards to drive the tissue culture seedling to move to the position 3mm above the mouth of the tissue culture bottle 442. Then the clamping jaw cylinder 435 is opened, the clamping jaw 436 is driven to open, and the tissue culture seedlings fall into the tissue culture bottles 442 under the action of gravity, so that the inoculation action is completed.

The invention also provides a tissue culture seedling inoculation method based on the device, as shown in fig. 16, comprising the following steps:

step one, taking out tissue culture seedlings from a tissue culture container, placing the tissue culture seedlings in a seedling placing tool 105 in a seedling placing station 1 at the first station, then controlling a clamping cylinder 101 to extend out to drive a seedling clamping plate 104 on the clamping cylinder to move in a centering manner, and placing the tissue culture Miao Gachi in a seedling clamping area;

step two, after the tissue culture seedling is clamped, driving the cam divider 7 to rotate by 90 degrees, at the moment, the tissue culture seedling clamped in the seedling placing station 1 arrives at the seedling cutting stations 2 at the two ends, then driving the locking cylinder 203 to retract, driving the first pressing block 206 to downwards compress on the seedling placing tool 105, and finishing the clamping of the tissue culture seedling in the vertical direction, simultaneously, driving the scissors cylinder 205 to downwards go right above the root and stem and leaf ends of the tissue culture seedling after the locking cylinder 203 is retracted, then driving the scissors cylinder 205 to act, closing scissors, and finishing the shearing action of the root and stem and leaf end of the tissue culture seedling; meanwhile, the seedling placing tool 105 at the original seedling taking, transplanting and inoculating station is rotated to the first station, and then the first step is repeated to finish the seedling placing action;

step three, driving the cam divider 7 to rotate 90 degrees again, moving the tissue culture seedlings to the middle-section seedling dividing station 3, driving the cutter Miao Qigang to drive the second pressing block 305 to move downwards to press the seedling placing tool 105 so as to clamp the tissue culture seedlings, then continuously moving the pushing plate 303 downwards, driving the blade 308 to continuously move downwards to the blade avoiding groove, and at the moment, finishing stem cutting of the tissue culture seedlings so as to divide the tissue culture seedlings into two; simultaneously, rotating the seedling placing tool for re-completing seedling feeding to the positions of two end seedling cutting stations 2 in the second step, and repeating the first step and the second step to complete the actions of re-feeding and head-tail seedling cutting of the tissue culture seedlings;

driving the cam divider 7 to rotate 90 degrees again, and moving the tissue culture seedlings to the seedling taking, transplanting and inoculating station 4; firstly, driving a traversing mechanism 42 to act, driving a clamping jaw 436 in a clamping mechanism 43 to move to a seedling grabbing position above a tissue culture Miao Zheng, then driving an air cylinder 433 to move downwards, driving the clamping jaw 436 to move downwards into a transplanting clamping jaw avoiding groove 1053 of a seedling placing tool 105, and driving a clamping jaw 436 to be closed by retracting a clamping jaw air cylinder 435 so as to realize the clamping action of the tissue culture seedlings; the clamping cylinder 101 is opened, the seedling clamping plates move to two sides, and the seedling placing tool 105 is opened to clamp the tissue culture seedlings; then the cylinder 433 is driven to move upwards, and the clamping jaw 436 is driven to move upwards to be separated from the seedling placing tool 105; the traversing mechanism 42 drives the clamping jaw 436 to move leftwards to be right above the inoculation station 44, and drives the air cylinder 433 to move downwards to drive the tissue culture seedling to move to a position 3mm above the opening of the tissue culture bottle 442; then the clamping jaw cylinder 435 is opened, the clamping jaw 436 is driven to open, and the tissue culture seedlings fall into the tissue culture bottle 442 under the action of gravity, so as to complete the inoculation action; repeating the first, second and third steps, and repeating the actions of seedling feeding, head-to-tail seedling cutting and seedling dividing;

step five, driving the cam divider 7 to rotate 90 degrees again after the tissue culture seedlings are inoculated, and returning the seedling placing station to the first station; and then repeating the first step to the fourth step to continuously perform the seedling separating inoculation action of the tissue culture seedlings.

In the description of the present invention, it should be noted that the directions or positional relationships indicated by the terms "center", "top", "bottom", "left", "right", "vertical", "horizontal", "inner", "outer", etc. are based on the directions or positional relationships shown in the drawings, are merely for convenience of describing the present invention and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

The principles and embodiments of the present invention have been described in detail with reference to specific examples, which are provided to facilitate understanding of the method and core ideas of the present invention; also, it is within the scope of the present invention to be modified by those of ordinary skill in the art in light of the present teachings. In view of the foregoing, this description should not be construed as limiting the invention.

Claims (10)

1. The tissue culture seedling inoculation device is characterized by comprising a rack;

a rotating part arranged on the frame;

the seedling placing station is arranged on the rotating part and can drive the seedling placing station to rotate around the vertical axis of the rotating part, and the seedling placing station is used for placing tissue culture seedlings;

two ends of the seedling cutting stations are arranged on the frame and are positioned outside the rotating part, and can cut stem leaves of tissue culture Miao Duanbu and roots;

the middle seedling separating station is arranged on the frame and positioned at the outer side of the rotating part, and can cut along the stem direction of the tissue culture seedling to divide the tissue culture seedling into two parts;

the seedling taking, transplanting and inoculating station is arranged on the frame and positioned at the outer side of the rotating part, and can clamp the tissue culture seedlings cut on the seedling placing station through clamping jaws and transplant the tissue culture seedlings into the tissue culture bottles to finish inoculation.

2. The tissue culture seedling inoculation device according to claim 1, wherein the rack comprises a supporting frame, a mounting plate is fixedly arranged at the top of the supporting frame, and the rotating part, the two-end seedling cutting stations, the middle-section seedling dividing station and the seedling taking and transplanting inoculation station are respectively arranged on the mounting plate; the bottom of the supporting frame is provided with a plurality of rollers.

3. The tissue culture seedling inoculation device according to claim 1, further comprising a finished product material frame which is arranged on the frame and is positioned obliquely below one end of the seedling taking, transplanting and inoculation station and used for placing a tissue culture bottle after the tissue culture seedling is inoculated.

4. The tissue culture seedling inoculation device according to claim 1, wherein the rotating part comprises a cam divider arranged on the frame, a mounting table is arranged at the top of the cam divider, and a plurality of seedling placing stations are uniformly arranged on the mounting table.

5. The tissue culture seedling inoculation device according to claim 1, wherein the seedling placing station comprises a seedling placing tool fixedly arranged on the rotating part, a trapezoid opening is formed in the top of the seedling placing tool, a blade avoiding groove is formed in the bottom of the trapezoid opening, and the blade avoiding groove is used for placing tissue culture seedlings; the seedling placing tool is provided with a seedling clamping plate avoiding groove and a transplanting clamping jaw avoiding groove which are arranged in parallel, and the clamp Miao Ban avoiding groove and the transplanting clamping jaw avoiding groove are both vertically arranged with the blade avoiding groove; the seedling placing tool is characterized in that seedling clamping plates are symmetrically arranged on two sides of the seedling placing tool, an opening is formed in one end of each seedling clamping plate, a first connecting plate is connected to the other end of each seedling clamping plate through a first spring, each first connecting plate is connected with a clamping cylinder, and when the clamping cylinders shrink, the seedling clamping plates can be driven to be inserted into the clamps Miao Ban to avoid the grooves to realize centering clamping of tissue culture seedlings.

6. The tissue culture seedling inoculation device according to claim 5, wherein the two-end seedling cutting stations comprise a supporting plate fixedly arranged on the frame, a locking cylinder is connected to the supporting plate through a locking cylinder mounting plate, a second connecting plate is fixedly arranged at the tail end of a cylinder rod of the locking cylinder, a first pressing block is mounted on the second connecting plate, the first pressing block is integrally trapezoid and can be matched with a trapezoid opening of the seedling placing tool, an opening groove is formed in the first pressing block, and the vertical projection of the opening groove coincides with the vertical projection of the clamp Miao Ban avoiding groove; the front end and the rear end of the first pressing block are symmetrically provided with scissor cylinders, the bottoms of cylinder rods of the scissor cylinders are connected with scissors, and the scissor cylinders can drive the scissors to be closed to finish shearing actions of root parts and stem rod end parts of tissue culture seedlings.

7. The tissue culture seedling inoculating device of claim 6, wherein said two-end seedling cutting station further comprises a waste collection mechanism; the waste collection mechanism comprises a waste collection frame and a guide wheel plate arranged on the frame, wherein a guide wheel is movably arranged on the guide wheel plate, and the guide wheel is arranged at the bottom of the waste collection frame and can drive the waste collection frame to move to the position below the seedling placing stations at the two ends of the seedling cutting stations; the end part of the waste material collecting frame is fixedly connected with a handle.

8. The tissue culture seedling inoculation device according to claim 5, wherein the middle section seedling dividing station comprises a support frame fixedly arranged on the frame, a cutter Miao Qigang is arranged on one side of the top of the support frame, a pushing plate is connected to a cylinder rod at the bottom of the cutter Miao Qigang, a plurality of guide shafts are fixedly connected to the bottom of the pushing plate, a second pressing block is arranged below the pushing plate, a plurality of guide bushings are arranged on the second pressing block, the guide shafts penetrate through the guide bushings, the size of a limiting part at the bottom of the guide shafts is larger than the inner diameter of the guide bushings, a blade is fixedly arranged at the bottom of the pushing plate, and the blade penetrates through a strip-shaped hole formed in the second pressing block; the guide shaft is sleeved with a second spring, and the second spring is positioned between the push plate and the second pressing block; the second pressing block is integrally trapezoidal and can be matched with a trapezoid opening of the seedling placing tool, a second pressing block clamp Miao Ban avoiding groove and a second pressing block blade avoiding groove are formed in the second pressing block, the second pressing block clamp Miao Ban avoiding groove is correspondingly arranged with the clamp Miao Ban avoiding groove, and the second pressing block blade avoiding groove is correspondingly arranged with the blade avoiding groove; the second pressing block is provided with a guide bushing mounting hole, and the guide bushing is fixedly arranged in the guide bushing mounting hole.

9. The tissue culture seedling inoculation device according to claim 1, wherein the seedling taking, transplanting and inoculation station comprises a seedling taking, transplanting and inoculation frame fixedly arranged on the frame, a traversing mechanism is arranged at the top of the seedling taking, transplanting and inoculation frame, a clamping mechanism is arranged at the inner side of the traversing mechanism, and the traversing mechanism can drive the clamping mechanism to traverse; an inoculation station is arranged on the inner side of the seedling taking, transplanting and inoculating machine frame, the inoculation station comprises a bottle rack fixedly arranged on the machine frame, and a tissue culture bottle is placed on the bottle rack; the clamping mechanism comprises a mounting bottom plate vertically arranged on the transverse moving mechanism, a guide rail is arranged on one side of the mounting bottom plate, a clamping jaw cylinder mounting plate is arranged on the guide rail in a sliding mode, one end of the clamping jaw cylinder mounting plate is connected with a driving cylinder, a clamping jaw cylinder is arranged on the clamping jaw cylinder mounting plate, clamping jaws are arranged at the bottoms of the clamping jaw cylinders, the clamping jaws can be driven to open or close by the clamping jaw cylinders, and clamping jaw pads are arranged on the inner sides of the clamping jaws.

10. The inoculation method of the tissue culture seedling is characterized by comprising the following steps of:

step one, taking out tissue culture seedlings from a tissue culture container, placing the tissue culture seedlings in a seedling placing tool in a seedling placing station at the first station, controlling a clamping cylinder to extend out, driving a seedling clamping plate on the clamping cylinder to move in a centering manner, and placing the tissue culture Miao Gachi in a seedling clamping area;

step two, after the tissue culture seedling is clamped, driving the cam divider to rotate by 90 degrees, at the moment, the tissue culture seedling clamped in the seedling placing station arrives at the seedling cutting stations at the two ends, then driving the locking cylinder to retract, driving the first pressing block to downwards compress on the seedling placing tool to clamp the tissue culture seedling in the vertical direction, simultaneously, driving the scissor cylinder to downwards move right above the root and stem leaf end of the tissue culture seedling after the locking cylinder is retracted, and then driving the scissor cylinder to act, closing the scissors to finish the shearing action of the root and stem end of the tissue culture seedling; simultaneously, the seedling placing tool originally positioned at the seedling taking, transplanting and inoculating station is rotated to the first station, and then the first step is repeated to finish the seedling placing action;

step three, driving the cam divider to rotate 90 degrees again, moving the tissue culture seedlings to a middle seedling dividing station, driving the cutter Miao Qigang to drive the second pressing block to move downwards to press the seedling placing tool so as to clamp the tissue culture seedlings, then continuously moving the pushing plate downwards, driving the blade to continuously move downwards to the blade avoiding groove, and at the moment, finishing stem cutting of the tissue culture seedlings, so that the tissue culture seedlings are divided into two parts; simultaneously, rotating the seedling placing tool for re-completing seedling feeding to the positions of two end seedling cutting stations in the second step, and repeating the first step and the second step to complete the actions of re-feeding and head-to-tail seedling cutting of the tissue culture seedlings;

driving the cam divider to rotate 90 degrees again, and moving the tissue culture seedlings to a seedling taking, transplanting and inoculating station; firstly, driving a transverse moving mechanism to act, driving clamping jaws in a clamping mechanism to move to a seedling grabbing position above tissue culture Miao Zheng, then driving an air cylinder to move downwards to drive the clamping jaws to move downwards to a seedling placing tool transplanting clamping jaw avoiding groove, and driving the clamping jaws to retract to drive the clamping jaws to close so as to realize tissue culture seedling clamping action; the clamping cylinder is opened, the seedling clamping plates move to two sides, and the seedling opening tool is opened to clamp the tissue culture seedlings; then driving the air cylinder to move upwards to drive the clamping jaw to move upwards to separate from the seedling placing tool; the transverse moving mechanism drives the clamping jaw to move leftwards to be right above the inoculation station, and drives the air cylinder to move downwards to drive the tissue culture seedling to move to a position 3mm above the opening of the tissue culture bottle; then the clamping jaw cylinder is opened to drive the clamping jaw to open, and the tissue culture seedling falls into the tissue culture bottle under the action of gravity to finish the inoculation action; repeating the first, second and third steps, and repeating the actions of seedling feeding, head-to-tail seedling cutting and seedling dividing;

step five, driving the cam divider to rotate 90 degrees again after the tissue culture seedlings are inoculated, and returning the seedling placing station to the first station; and then repeating the first step to the fourth step to continuously perform the seedling separating inoculation action of the tissue culture seedlings.