CN117417822A - Sample adding detection equipment applied to mycoplasma culture and detection method thereof - Google Patents

Abstract

The invention relates to the technical field of mycoplasma culture detection, in particular to a sample adding detection device and a detection method applied to mycoplasma culture. Comprising the following steps: the incubator is provided with a top frame above, and the front side of the incubator is provided with a switch door; the loading assembly is arranged in the incubator and is in sliding connection with the incubator; through the airtight structure of switch door and incubator for the incubator internal environment is fixed, and through sealed structure of filling up, make form sealed environment between fly frame and the mount branch, make the switch door open the back, external material gets into the incubator in, but can't get into in the culture dish, after the incubator internal circulation structure discharges harmful substance, at the structure through second gear and ring gear, open the fly frame, make the culture dish contact culture environment in proper order, avoid harmful substance to get into to mycoplasma culture environment in, inhibit the culture of mycoplasma.

Description

Sample adding detection equipment applied to mycoplasma culture and detection method thereof

Technical Field

The invention relates to the technical field of mycoplasma culture detection, in particular to a sample adding detection device and a detection method applied to mycoplasma culture.

Background

Mycoplasma is a kind of micro-organism with the smallest procaryotic cell type, which has no cell wall, high polymorphism, can pass through the bacteria filter and can be cultured and proliferated by using artificial culture medium, and the size is 0.1-0.3 micrometer. Because of its ability to form both filamentous and branched shapes, it is known as mycoplasma. Mycoplasma widely exist in human and animal bodies, are mostly non-pathogenic, and are mainly mycoplasma pneumoniae, ureaplasma urealyticum, mycoplasma hominis, mycoplasma genitalium and the like.

In the prior art, the most accurate detection method of mycoplasma is a culture detection method, namely, an obtained sample is put into a culture medium, and is cultured for a certain period in a proper environment, and finally, whether mycoplasma exists in the sample is obtained according to observation.

However, since mycoplasma is required to be cultured in a culture dish for a long time and in a fixed culture environment, and is often carried out in hospitals, a sterilizing substance is easily introduced into the mycoplasma culture environment along with the taking and placing of the culture dish by medical staff, and the culture of mycoplasma is inhibited.

Disclosure of Invention

The invention aims to provide a mycoplasma culture sample-adding detection device and a detection method thereof, which are applied to solve the problems of the background technology.

In order to achieve the above purpose, the present invention provides the following technical solutions:

a sample-adding detection device for mycoplasma culture and a detection method thereof, comprising:

the incubator is provided with a top frame above, and the front side of the incubator is provided with a switch door;

the loading assembly is arranged in the incubator and is in sliding connection with the incubator;

the transmission assembly is arranged at the rear side of the incubator and drives the loading assembly to move;

the rotating shaft is arranged below the transmission assembly and is movably connected with the transmission assembly, and the rear side of the rotating shaft is in transmission connection with the external motor and the oil cylinder;

the first rotating rod is arranged above the front side of the rotating shaft, a second rotating rod is sleeved in the first rotating rod, and the first rotating rod is connected with the second rotating rod in a rotating way;

the lifting seat is arranged in the top frame and is vertically and slidably connected with the top frame.

Preferably, the front side of incubator rotates and is connected with the switch door, and the rear surface outer edge of switch door is provided with the sealing strip, and the switch door is closed the back with the incubator, through sealing strip sealing connection, and the middle part of switch door sets up to observation glass, fixedly connected with handle on the front side right middle part of switch door.

Preferably, the rear side inner wall of incubator is provided with the movable groove, and the movable groove sets up to the polygon, and sliding connection has the loading assembly before the movable groove, and the loading assembly includes the movable block, and the movable block sets up in the movable groove to with movable groove sliding connection, the front side middle part fixedly connected with connecting rod of movable block, the front side tip fixedly connected with fly leaf of connecting rod.

Preferably, the top middle part of fly leaf is provided with the standing groove, has placed the culture dish in the standing groove, and the top fixedly connected with mount of standing groove, and the mount sets up to semi-circular, and the top outside of mount is provided with the fly frame, and the fly frame sets up to semi-circular, is provided with sealed the pad on the one side inner wall of setting up the opening of fly frame, and the below rotation of fly frame is installed on the fly leaf, and the below outer wall of fly frame is provided with the ring gear, and the middle part top of fly frame is provided with the sampling pipe, the below of sampling pipe and the below UNICOM of fly frame, and the top of sampling pipe is provided with the sealing block.

Preferably, the transmission assembly comprises a first belt, the first belt is arranged to be polygonal identical to the movable groove, a first driving wheel is arranged in an inflection point below the first belt, an expanding wheel is arranged in other inflection points of the first belt, the first driving wheel and the expanding wheel are in meshed transmission with the first belt, a plurality of connecting blocks are fixedly connected to the outer wall of the first belt, the front side of each connecting block extends out of the range of the first belt, a sleeve is fixedly connected to the inner wall of the front side of each connecting block, an opening cavity structure is arranged on the inner side of each sleeve, a sliding rod is connected to the inner side of each sleeve in a sliding mode, a fixing ring is fixedly connected to the inner side end portion of each sliding rod, and the fixing rings are sleeved on the connecting rods and are in rotary connection with the connecting rods.

Preferably, the middle part of first drive wheel is provided with the through-hole, is provided with a plurality of evenly distributed's spline groove on the inner wall of through-hole, swing joint has the pivot in the through-hole, fixedly connected with a plurality of splines on the outer wall of pivot, spline and spline groove joint, and the front side of spline is provided with the baffle, baffle and the outer wall fixed connection of pivot, the rear side of baffle and the front side middle part butt of first drive wheel, fixedly connected with worm on the front side outer wall of pivot.

Preferably, the right side of the worm is provided with a worm wheel, the worm wheel is in meshed transmission with the worm, the lower part of the worm wheel is in rotary connection with the inner wall of the bottom of the incubator, a fifth transmission wheel is fixedly connected above the worm wheel, a fourth transmission wheel is arranged on the left side of the fifth transmission wheel, a third belt is sleeved on the fifth transmission wheel and the fourth transmission wheel and is in transmission connection with the fifth transmission wheel and the fourth transmission wheel, a second rotating rod is fixedly connected above the fourth transmission wheel, a connecting seat is arranged above the fourth transmission wheel, the connecting seat is L-shaped, the lower left side of the connecting seat is fixedly connected with the bottom of the incubator, the second rotating rod vertically penetrates through the middle part of the connecting seat, and a screw rod is arranged at the upper end of the second rotating rod.

Preferably, the outside cover of second dwang is equipped with first dwang, the lower extreme of first dwang rotates with the upper surface of connecting seat to be connected, fixedly connected with second drive wheel on the bottom outer wall of first dwang, the right side of second drive wheel is provided with the third drive wheel, the cover is equipped with the second belt on second drive wheel and the third drive wheel, the second belt is connected with second drive wheel and the transmission of third drive wheel, the below fixedly connected with first gear of third drive wheel, the first gear is unanimous with the height of pivot, worm and first gear drive are connected, fixedly connected with second gear on the top outer wall of first dwang, second gear and ring gear meshing transmission.

Preferably, the top cover of lead screw is equipped with the lift post, and the inner wall and the lead screw spiro union of lift post, the upside of lift post and the front side below fixed connection of lift seat are provided with the sealing ring on the lateral wall of lift seat, and the lift seat passes through the vertical sliding connection of sealing ring and the inner wall of roof-rack, installs the microscope in the middle part of lift seat.

The detection method applied to mycoplasma culture sample-adding detection equipment comprises the following steps:

s1: opening a switch door, rotating a plurality of movable frames to enable the movable frames to face the openings of the fixed frames uniformly, enabling the openings on the front sides of the fixed frames to leak, placing mycoplasma culture dishes into a placing groove from the openings on the front sides of the movable frames and the fixed frames, reversely rotating the movable frames to enable the movable frames and the fixed frames to be staggered, and sealing and storing the culture dishes by using sealing gaskets on the inner sides of the movable frames;

s2: dripping samples into the culture dishes through the sample adding pipes, dripping different numbers of samples into different culture dishes so as to observe and compare, closing the sample adding pipes through the sealing blocks after the sample adding is completed, closing the switch door, and regulating the internal environment of the incubator so that the incubator is suitable for culturing mycoplasma in the culture medium in the culture dishes;

s3: after the culture medium is cultured for a certain time, an external motor is started, so that the rotating shaft drives the first driven wheel to rotate under the action of the spline and drives the first belt to drive, and the connecting block rotates the sleeve joint structure on the connecting rod under the action of the telescopic structure of the sleeve and the sliding rod and the fixed ring drives the movable plate to move along the polygonal direction of the movable plate at the front side of the movable groove until one movable plate moves to the highest point of the movable groove;

s4: the external oil cylinder is started, the rotating shaft is controlled to slide horizontally and forwards, so that a spline of the rotating shaft slides out of a spline groove, the worm is horizontally and forwards, in the process that the worm is horizontally and forwards, the first gear is driven to rotate by the worm through the first gear, the third driving wheel above the first gear controls the first rotating rod to rotate through the second driving wheel under the driving action of the second belt, the second gear above the first driving wheel rotates, and after the connecting plate rises to the highest point, the toothed ring is meshed with the second gear, so that the movable frame is overturned, and the upper part of the culture dish is leaked;

s5: the worm is meshed with the worm wheel after passing through the first gear, the second rotating rod is controlled to rotate along with the rotation of the rotating shaft, the motor rotates positively and negatively, the lifting column drives the lifting seat to move up and down, and the up-down position of the microscope is controlled, so that whether mycoplasma is generated in the culture medium or not is observed;

s6: the motor is reversely started, so that the lifting seat is controlled to ascend and reset by the rotation of the screw rod, the rotating shaft is controlled to backwards shrink through the oil cylinder, the first gear is driven to reversely rotate in the process, the second gear is controlled to reversely rotate the movable frame to reset, the rotating shaft is continuously controlled to backwards shrink until the spline is clamped into the spline groove, so that the culture dishes on the rest movable plates are moved to the highest point, and the mycoplasma culture condition in each culture dish is observed.

Compared with the prior art, the invention has the beneficial effects that: the environment in the incubator is fixed through the closed structure of the switch door and the incubator, and a sealing gasket structure is adopted to form a sealing environment between the movable frame and the fixed frame branch, so that after the switch door is opened, external substances enter the incubator but cannot enter the culture dish, after harmful substances are discharged through the internal circulation structure of the incubator, the movable frame is opened through the structures of the second gear and the toothed ring, the culture dish is sequentially contacted with the culture environment, the harmful substances are prevented from entering the mycoplasma culture environment, and the culture of mycoplasma is inhibited;

the transmission assembly is controlled to operate through a spline structure of the rotating shaft, and the loading assembly is sequentially lifted to the highest point, so that the second gear is meshed with the toothed ring, the movable frame is controlled to be opened, and the culture environment is enabled to enter the culture medium;

through the worm structure of pivot, at the in-process that the pivot was advanced, control first gear is rotatory to make the second gear rotate 180, make movable frame and mount opening coincidence just, and before the fly leaf moves, the pivot is retrieved and is made movable frame reverse rotation to reset, ensures that movable frame and mount keep closed state under the initial state.

Drawings

FIG. 1 is a schematic perspective view of the present invention;

FIG. 2 is a schematic view of the internal structure of the incubator of the present invention;

FIG. 3 is a schematic sectional view of the incubator of the present invention;

FIG. 4 is an enlarged schematic view of FIG. 3A in accordance with the present invention;

FIG. 5 is a schematic view of a loading assembly connection structure of the present invention;

FIG. 6 is an exploded view of the loading assembly of the present invention;

FIG. 7 is a schematic view of a movable frame structure according to the present invention;

FIG. 8 is a schematic view of the connection structure of the spindle and the first driving wheel of the present invention;

FIG. 9 is a schematic view of a first and second rotary lever connection structure according to the present invention;

FIG. 10 is an enlarged schematic view of FIG. 9B in accordance with the present invention;

fig. 11 is an exploded view of the connection of the first and second rotating rods according to the present invention.

In the figure: incubator 1, movable groove 11, top frame 12, switch door 2, seal bar 21, observation glass 22, handle 23, loading unit 3, movable plate 31, connecting rod 32, movable block 33, placement groove 34, fixed frame 35, movable frame 36, gasket 37, toothed ring 38, sampling tube 39, sealing block 310, transmission unit 4, first transmission wheel 41, spline groove 42, expansion wheel 43, first belt 44, connecting block 45, sleeve 46, slide bar 47, fixed ring 48, rotation shaft 5, retaining plate 51, spline 52, worm 53, connecting seat 6, first rotation rod 7, second transmission wheel 71, third transmission wheel 72, second belt 73, first gear 74, second gear 75, second rotation rod 8, fourth transmission wheel 81, fifth transmission wheel 82, third belt 83, worm wheel 84, lead screw 85, lifting seat 9, sealing ring 91, microscope 92, lifting column 93.

Detailed Description

In order to more clearly illustrate the general inventive concept, reference will be made in the following detailed description, by way of example, to the accompanying drawings.

It should be noted that in the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention, however, the present invention may be practiced in other ways than as described herein, and therefore the scope of the present invention is not limited by the specific embodiments disclosed below.

In addition, in the description of the present invention, it should be understood that the terms "center", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "axial", "radial", "circumferential", etc. indicate orientations or positional relationships based on the drawings, are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present invention.

In the present invention, unless explicitly specified and limited otherwise, the terms "mounted," "connected," "secured," and the like are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally formed; can be directly connected or indirectly connected through an intermediate medium, and can be communicated with the inside of two elements or the interaction relationship of the two elements. However, it is noted that a direct connection indicates that two bodies connected together do not form a connection relationship by an excessive structure, but are connected to form a whole by a connection structure. The specific meaning of the above terms in the present invention can be understood by those of ordinary skill in the art according to the specific circumstances.

In the present invention, unless expressly stated or limited otherwise, a first feature "up" or "down" a second feature may be the first and second features in direct contact, or the first and second features in indirect contact via an intervening medium. In the description of the present specification, a description referring to terms "one embodiment," "some embodiments," "examples," "specific examples," or "some examples," etc., means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the present invention. In this specification, schematic representations of the above terms are not necessarily directed to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

Embodiment one:

referring to fig. 1 to 11, the present invention provides a technical solution: a sample-adding detection device for mycoplasma culture and a detection method thereof, comprising: the incubator 1, the upper side of incubator 1 has roof rack 12, the front side of incubator 1 has switch door 2; the loading assembly 3 is arranged in the incubator 1 and is in sliding connection with the incubator 1; the transmission assembly 4 is arranged at the rear side of the incubator 1, and the transmission assembly 4 drives the loading assembly 3 to move; the rotating shaft 5 is arranged below the transmission assembly 4 and is movably connected with the transmission assembly 4, and the rear side of the rotating shaft 5 is in transmission connection with an external motor and an oil cylinder; the first rotating rod 7 is arranged above the front side of the rotating shaft 5, a second rotating rod 8 is sleeved in the first rotating rod 7, and the first rotating rod 7 is rotationally connected with the second rotating rod 8; the lifting seat 9, the lifting seat 9 is arranged in the top frame 12 and is vertically and slidably connected with the top frame 12.

Embodiment two:

the structure of the mycoplasma culture sample-adding detection device and the detection method thereof disclosed in the second embodiment of the invention are basically the same as those of the first embodiment, and the difference is that in order to keep a closed environment inside the incubator 1 and facilitate the observation of the inside, the front side of the incubator 1 is rotatably connected with a switch door 2, the outer edge of the rear surface of the switch door 2 is provided with a sealing strip 21, the switch door 2 is hermetically connected with the incubator 1 through the sealing strip 21 after being closed, the middle part of the switch door 2 is provided with an observation glass 22, and the middle part of the right side of the front side of the switch door 2 is fixedly connected with a handle 23.

The structure through handle 23 controls the opening and closing between switch door 2 and incubator 1 to through the structure of sealing strip 21, make switch door 2 closed after, incubator 1 and switch door 2 are closed completely, and through the structure of observation glass 22, the convenience is observed the incubator 1 inside.

Embodiment III:

the structure of the detection device and the detection method for mycoplasma culture sample application disclosed in the third embodiment of the invention are basically the same as those of the second embodiment, and the difference is that, in order to conveniently put a plurality of groups of culture dishes in the incubator 1 and increase different numbers of samples in different culture dishes, the inner wall of the rear side of the incubator 1 is provided with a movable groove 11, the movable groove 11 is arranged into a polygon, the front of the movable groove 11 is slidingly connected with a loading assembly 3, the loading assembly 3 comprises a movable block 33, the movable block 33 is arranged in the movable groove 11 and slidingly connected with the movable groove 11, the middle part of the front side of the movable block 33 is fixedly connected with a connecting rod 32, and the end part of the front side of the connecting rod 32 is fixedly connected with a movable plate 31. The upper middle part of fly leaf 31 is provided with standing groove 34, has placed the culture dish in the standing groove 34, and the top fixedly connected with mount 35 of standing groove 34, mount 35 set up to semi-circular, and the top outside of mount 35 is provided with fly leaf 36, and fly leaf 36 sets up to semi-circular, is provided with sealed 37 on the one side inner wall of the setting opening of fly leaf 36, and the below rotation of fly leaf 36 is installed on fly leaf 31, and the below outer wall of fly leaf 36 is provided with ring gear 38, and the middle part top of fly leaf 36 is provided with the sampling tube 39, and the below of sampling tube 39 and the below UNICOM of fly leaf 36 are provided with sealing block 310 to the top of sampling tube 39.

Through the structure that movable block 33 slides in movable tank 11 for movable plate 31 slides along movable tank 11, and movable frame 36 and mount 35 are the semicircular structure that sets up the opening, and movable frame 36 sets up in the outside of mount 35, when making movable frame 36 rotate to both openings the same direction, spill standing groove 34, so that put the culture dish into, when movable frame 36 rotates to both openings opposite direction, seal both junction through sealed 37, make the culture dish keep in the confined environment, through the structure of sampling tube 39, sample application is carried out in the culture dish, and after the sample application is accomplished, close sampling tube 39 through the structure of sealed block 310, ensure the closure in movable frame 36 and the mount 35.

Embodiment four:

the structure of the mycoplasma culture sample-adding detection device and the detection method thereof disclosed in the fourth embodiment of the invention are basically the same as those of the third embodiment, and the difference is that, in order to move different culture dishes above the incubator 1 to observe the culture conditions of mycoplasma in the culture dishes, the transmission assembly 4 comprises a first belt 44, the first belt 44 is arranged in the same polygon as the movable groove 11, a first transmission wheel 41 is arranged in the inflection point below the first belt 44, an expansion wheel 43 is arranged in the other inflection points of the first belt 44, the first transmission wheel 41 and the expansion wheel 43 are in meshed transmission with the first belt 44, a plurality of connecting blocks 45 are fixedly connected to the outer wall of the first belt 44, the front side of each connecting block 45 extends out of the range of the first belt 44, a sleeve 46 is fixedly connected to the inner wall of the front side of each connecting block 45, the sleeve 46 is in a cavity structure with an opening, a sliding rod 47 is slidably connected in the cavity of the sleeve 46, the inner end of the sliding rod 47 is fixedly connected with a fixed ring 48, and the fixed ring 48 is sleeved on the connecting rod 32 and is rotatably connected with the connecting rod 32.

The first belt 44 is expanded into a polygonal structure corresponding to the movable groove 11 through the structures of the expansion wheels 43, the first belt 44 is driven to operate through the first driving wheel 41, the first belt 44 enables the connecting block 45 to move along the outer side of the movable groove 11 in the operation process, and the fixed ring 48 pulls the movable block 33 to move along the movable groove 11 under the action of the telescopic structure formed by the sleeve 46 and the sliding rod 47.

Fifth embodiment:

the structure of the mycoplasma culture sample-adding detection device and the detection method thereof disclosed in the fifth embodiment of the invention is basically the same as that of the fourth embodiment, and the difference is that, in order to enable the culture dish to rise to the highest point, the movable frame 36 above the culture dish can be automatically opened, the upper and lower heights of the microscope 92 are conveniently adjusted for observation, the middle part of the first driving wheel 41 is provided with a through hole, the inner wall of the through hole is provided with a plurality of spline grooves 42 which are uniformly distributed, a rotating shaft 5 is movably connected in the through hole, the outer wall of the rotating shaft 5 is fixedly connected with a plurality of splines 52, the splines 52 are clamped with the spline grooves 42, the front side of the splines 52 is provided with a baffle plate 51, the baffle plate 51 is fixedly connected with the outer wall of the rotating shaft 5, the rear side of the baffle plate 51 is abutted with the middle part of the front side of the first driving wheel 41, and the outer wall of the front side of the rotating shaft 5 is fixedly connected with a worm 53.

Through the joint structure of spline 52 and spline groove 42 for external motor drive pivot 5 carries out pivoted in-process, drives first drive wheel 41 and rotates, and after pivot 5 moves forward, spline 52 drops in from spline groove 42, and the rotation of pivot 5 can not drive first rotation wheel 41 and rotate this moment.

The right side of worm 53 is provided with worm wheel 84, worm wheel 84 and worm 54 meshing transmission, the below of worm wheel 84 is connected with the bottom inner wall rotation of incubator 1, the top fixedly connected with fifth drive wheel 82 of worm wheel 84, the left side of fifth drive wheel 82 is provided with fourth drive wheel 81, the cover is equipped with third belt 83 on fifth drive wheel 82 and the fourth drive wheel 81, third belt 83 and fifth drive wheel 82 and fourth drive wheel 81 transmission are connected, the top fixedly connected with second dwang 8 of fourth drive wheel 81, the top of fourth drive wheel 81 is provided with connecting seat 6, connecting seat 6 sets up to the L type, the left side below of connecting seat 6 and the bottom fixed connection of incubator 1, second dwang 8 vertically runs through the middle part of connecting seat 6, the upper end of second dwang 8 is provided with lead screw 85. The top cover of lead screw 85 is equipped with lift post 93, and lift post 93's inner wall and lead screw 85 spiro union, and lift post 93's top and the front side below fixed connection of lift seat 9 are provided with sealing ring 91 on the lateral wall of lift seat 9, and lift seat 9 passes through sealing ring 91 and the vertical sliding connection of inner wall of roof-rack 12, installs microscope 92 in the middle part of lift seat 9.

After the rotating shaft 5 moves forwards, the worm 53 at the front side of the rotating shaft is meshed with the worm wheel 84, so that the worm wheel 84 and the fifth driving wheel 82 are driven to rotate in the rotating process of the worm 53, under the driving action of the third belt 83, the fourth driving wheel 81 drives the second rotating rod 8 to rotate, the screw rod 85 drives the lifting column 93 to move up and down, the up-down position of the microscope 92 on the lifting seat 9 is adjusted, the microscope 92 can directly observe the culture condition of mycoplasma in a culture dish, and the lifting seat 9 is kept sealed with the top frame 12 in the up-down sliding process through the structure of the sealing ring 91 arranged on the side wall of the lifting seat 9, so that the sealing performance in the incubator 1 is ensured.

The outside cover of second dwang 8 is equipped with first dwang 7, the lower extreme of first dwang 7 is connected with the upper surface rotation of connecting seat 6, fixedly connected with second drive wheel 71 on the bottom outer wall of first dwang 7, the right side of second drive wheel 71 is provided with third drive wheel 72, the cover is equipped with second belt 73 on second drive wheel 71 and the third drive wheel 72, second belt 73 is connected with second drive wheel 71 and third drive wheel 72 transmission, the below fixedly connected with first gear 74 of third drive wheel 72, first gear 74 is unanimous with the height of pivot 5, worm 53 is connected with first gear 74 transmission, fixedly connected with second gear 75 on the top outer wall of first dwang 7, second gear 75 and ring 38 meshing transmission.

In the process of sliding the rotating shaft 5 forwards and backwards, the worm 53 pushes the second gear 75 to rotate forwards and backwards, and under the transmission action of the second transmission wheel 71, the third transmission wheel 72 and the second belt 73, the first rotating rod 7 controls the forward and reverse rotation of the second gear 75, so that the highest movable frame 36 and the fixed frame 35 are closed and opened.

The scheme is specifically as follows: opening the switch door 2, rotating a plurality of movable frames 36 by 180 degrees to enable the movable frames 36 to face the openings of the fixed frames 35 uniformly, enabling the front openings of the fixed frames 35 to leak out, placing mycoplasma culture dishes into the placing grooves 34 from the front openings of the movable frames 36 and the fixed frames 35, reversely rotating a plurality of movable frames 36 by 180 degrees to enable the movable frames 36 and the fixed frames 35 to be staggered, and sealing and storing the culture dishes by using sealing gaskets 37 at the inner sides of the movable frames 36; dropping samples into the culture dishes through the sample adding pipes 39, dropping different numbers of samples into different culture dishes so as to observe and compare, closing the sample adding pipes 39 through the sealing blocks 310 after the sample adding is completed, closing the switch door 2, and adjusting the internal environment of the incubator 1 so as to be suitable for culturing mycoplasma by the culture medium in the culture dishes; after the culture medium is cultured for a certain time, an external motor is started, so that the rotating shaft 5 drives the first driven wheel 41 to rotate under the action of the spline 52 and drives the first belt 44 to drive, so that the connecting block 45 rotates the sleeve joint structure on the connecting rod 32 under the action of the telescopic structure of the sleeve 46 and the sliding rod 47, and the fixed ring 48 drives the movable plate 31 to move along the polygonal direction of the movable groove 11 until one movable plate 31 moves to the highest point of the movable groove 11; the external oil cylinder is started, the rotating shaft 5 is controlled to slide horizontally forwards, the spline 52 of the rotating shaft 5 slides out of the spline groove 42, the worm 53 moves horizontally forwards, in the horizontal forward process of the worm 53, the worm 53 drives the first gear 75 to rotate through the first gear 75, the third driving wheel 72 above the first gear 75 controls the first rotating rod 7 to rotate through the second driving wheel 71 under the driving action of the second belt 73, the second gear 75 above the first driving wheel rotates, and after the connecting plate 31 rises to the highest point, the toothed ring 38 is meshed with the second gear 75, so that the movable frame 36 overturns, and the upper part of the culture dish leaks out; the worm 53 is meshed with the worm gear 84 after passing through the first gear 74, and along with the rotation of the rotating shaft 5, the second rotating rod 8 is controlled to rotate, and the motor rotates positively and negatively, so that the lifting column 93 drives the lifting seat 9 to move up and down, and the up and down position of the microscope 92 is controlled, so that whether mycoplasma is generated in the culture medium is observed; the motor is reversely started, so that the lifting seat 9 is controlled to ascend and reset by the rotation of the screw rod 85, the rotating shaft 5 is controlled to shrink backwards through the oil cylinder, the first gear 74 is driven to reversely rotate in the process, the second gear 75 is controlled to reversely rotate 180 degrees to reset the movable frame 36, the rotating shaft 5 is continuously controlled to shrink backwards until the spline 52 is clamped into the spline groove 42, so that culture dishes on the rest movable plates 31 are moved to the highest point, and mycoplasma culture conditions in each culture dish are observed.

Those of ordinary skill in the art will appreciate that: the discussion of any of the embodiments above is merely exemplary and is not intended to suggest that the scope of the invention (including the claims) is limited to these examples; the technical features of the above embodiments or in the different embodiments may also be combined within the idea of the invention, the steps may be implemented in any order and there are many other variations of the different aspects of the invention as above, which are not provided in detail for the sake of brevity.

The present invention is intended to embrace all such alternatives, modifications and variances which fall within the broad scope of the appended claims. Therefore, any omission, modification, equivalent replacement, improvement, etc. of the present invention should be included in the scope of the present invention.

Claims (10)

1. Be applied to mycoplasma culture application of sample check out test set, characterized by comprising:

the incubator comprises an incubator (1), wherein a top frame (12) is arranged above the incubator (1), and a switch door (2) is arranged at the front side of the incubator (1);

the loading assembly (3) is arranged in the incubator (1) and is in sliding connection with the incubator (1);

the transmission assembly (4) is arranged at the rear side of the incubator (1), and the transmission assembly (4) drives the loading assembly (3) to move;

the rotating shaft (5) is arranged below the transmission assembly (4) and is movably connected with the transmission assembly (4), and the rear side of the rotating shaft (5) is in transmission connection with an external motor and an oil cylinder;

the first rotating rod (7) is arranged above the front side of the rotating shaft (5), a second rotating rod (8) is sleeved in the first rotating rod (7), and the first rotating rod (7) is rotationally connected with the second rotating rod (8);

the lifting seat (9) is arranged in the top frame (12) and is vertically and slidably connected with the top frame (12).

2. The sample addition detection device for mycoplasma culture of claim 1, wherein the front side of the incubator (1) is rotationally connected with the switch door (2), a sealing strip (21) is arranged on the outer edge of the rear surface of the switch door (2), the switch door (2) is in sealing connection with the sealing strip (21) after the incubator (1) is closed, the middle part of the switch door (2) is provided with an observation glass (22), and a handle (23) is fixedly connected to the middle part of the right of the front side of the switch door (2).

3. The sample addition detection device for mycoplasma culture of claim 2, wherein a movable groove (11) is formed in the inner wall of the rear side of the incubator (1), the movable groove (11) is polygonal, the front side of the movable groove (11) is slidably connected with the loading assembly (3), the loading assembly (3) comprises a movable block (33), the movable block (33) is arranged in the movable groove (11) and is slidably connected with the movable groove (11), a connecting rod (32) is fixedly connected with the middle part of the front side of the movable block (33), and a movable plate (31) is fixedly connected with the front side end part of the connecting rod (32).

4. The sample addition detection device for mycoplasma culture according to claim 3, wherein a placing groove (34) is formed in the middle of the upper portion of the movable plate (31), a culture dish is placed in the placing groove (34), a fixing frame (35) is fixedly connected to the upper portion of the placing groove (34), the fixing frame (35) is arranged in a semicircular shape, a movable frame (36) is arranged on the outer side of the upper portion of the fixing frame (35), a sealing gasket (37) is arranged on the inner wall of one side of the movable frame (36) with an opening, the lower portion of the movable frame (36) is rotatably mounted on the movable plate (31), a toothed ring (38) is arranged on the outer wall of the lower portion of the movable frame (36), a sample addition pipe (39) is arranged above the middle of the movable frame (36), the lower portion of the sample addition pipe (39) is communicated with the lower portion of the movable frame (36), and a sealing block (310) is arranged above the sample addition pipe (39).

5. The sample application and culture detection device for mycoplasma culture according to claim 4, wherein the transmission assembly (4) comprises a first belt (44), the first belt (44) is arranged to be polygonal with the movable groove (11), a first transmission wheel (41) is arranged in a lower inflection point of the first belt (44), an expansion wheel (43) is arranged in other inflection points of the first belt (44), the first transmission wheel (41) and the expansion wheel (43) are in meshed transmission with the first belt (44), a plurality of connecting blocks (45) are fixedly connected to the outer wall of the first belt (44), the front side of each connecting block (45) extends out of the range of the first belt (44), a sleeve (46) is fixedly connected to the front side inner wall of each connecting block (45), the sleeve (46) is of a hollow structure with an opening arranged inside, a sliding rod (47) is connected in a hollow cavity of each sleeve (46) in a sliding mode, a fixing ring (48) is fixedly connected to the inner side end portion of each sliding rod (47), and the fixing ring (48) is fixedly connected to each connecting rod (32).

6. The sample application detection device for mycoplasma culture of claim 5, wherein a through hole is formed in the middle of the first driving wheel (41), a plurality of spline grooves (42) which are uniformly distributed are formed in the inner wall of the through hole, a rotating shaft (5) is movably connected in the through hole, a plurality of splines (52) are fixedly connected to the outer wall of the rotating shaft (5), the splines (52) are clamped with the spline grooves (42), a baffle (51) is arranged on the front side of the splines (52), the baffle (51) is fixedly connected with the outer wall of the rotating shaft (5), the rear side of the baffle (51) is abutted to the middle of the front side of the first driving wheel (41), and a worm (53) is fixedly connected to the outer wall of the front side of the rotating shaft (5).

7. The sample addition detection device for mycoplasma culture according to claim 6, wherein a worm wheel (84) is arranged on the right side of the worm (53), the worm wheel (84) is in meshed transmission with the worm (54), the lower part of the worm wheel (84) is rotatably connected with the inner wall of the bottom of the incubator (1), a fifth driving wheel (82) is fixedly connected with the upper part of the worm wheel (84), a fourth driving wheel (81) is arranged on the left side of the fifth driving wheel (82), a third belt (83) is sleeved on the fifth driving wheel (82) and the fourth driving wheel (81), the third belt (83) is in transmission connection with the fifth driving wheel (82) and the fourth driving wheel (81), a second rotating rod (8) is fixedly connected with the upper part of the fourth driving wheel (81), a connecting seat (6) is arranged on the upper part of the fourth driving wheel (81), the lower left side of the connecting seat (6) is fixedly connected with the bottom of the incubator (1), and the second rotating rod (8) is fixedly connected with the middle part of the second rotating rod (85).

8. The sample culture and application detection device applied to mycoplasma as claimed in claim 7, wherein a first rotating rod (7) is sleeved on the outer side of the second rotating rod (8), the lower end of the first rotating rod (7) is rotationally connected with the upper surface of the connecting seat (6), a second driving wheel (71) is fixedly connected to the outer wall of the bottom of the first rotating rod (7), a third driving wheel (72) is arranged on the right side of the second driving wheel (71), a second belt (73) is sleeved on the second driving wheel (71) and the third driving wheel (72), the second belt (73) is in driving connection with the second driving wheel (71) and the third driving wheel (72), a first gear (74) is fixedly connected to the lower portion of the third driving wheel (72), the first gear (74) is consistent with the height of the rotating shaft (5), a worm (53) is in driving connection with the first gear (74), a second gear (75) is fixedly connected to the outer wall of the top of the first rotating rod (7), and the second gear (75) is meshed with the second gear (38).

9. The sample addition detection device for mycoplasma culture of claim 7, wherein a lifting column (93) is sleeved above the screw rod (85), the inner wall of the lifting column (93) is in threaded connection with the screw rod (85), the upper part of the lifting column (93) is fixedly connected with the lower front side of the lifting seat (9), a sealing ring (91) is arranged on the side wall of the lifting seat (9), the lifting seat (9) is vertically and slidably connected with the inner wall of the top frame (12) through the sealing ring (91), and a microscope (92) is arranged in the middle part of the lifting seat (9).

10. The method for detecting mycoplasma culture and sample application according to any one of claims 1 to 10, wherein the detection method of the detection apparatus comprises the steps of:

s1: opening the switch door (2), rotating a plurality of movable frames (36) for 180 degrees, enabling the movable frames (36) to face the opening of the fixed frame (35) uniformly, enabling the front opening of the fixed frame (35) to leak out, placing the mycoplasma culture dish into the placing groove (34) from the front openings of the movable frames (36) and the fixed frame (35), reversely rotating the movable frames (36) for 180 degrees, enabling the movable frames (36) and the fixed frame (35) to be staggered, and sealing and storing the culture dish by using a sealing gasket (37) at the inner side of the movable frames (36);

s2: dropping samples into the culture dishes through the sample adding pipes (39), dropping different numbers of samples into different culture dishes so as to observe and compare, closing the sample adding pipes (39) through the sealing blocks (310) after the sample adding is completed, closing the switch door (2), and adjusting the internal environment of the incubator (1) to ensure that the incubator is suitable for culturing mycoplasma by the culture medium in the culture dishes;

s3: after the culture medium is cultured for a certain time, an external motor is started, so that the rotating shaft (5) drives the first driven wheel (41) to rotate under the action of the spline (52) and drives the first belt (44) to drive, the connecting block (45) rotates the sleeve structure on the connecting rod (32) under the action of the telescopic structure of the sleeve (46) and the sliding rod (47), and the fixed ring (48) drives the movable plate (31) to move along the polygonal direction of the movable groove (11) until one movable plate (31) moves to the highest point of the movable groove (11);

s4: the external oil cylinder is started, the rotating shaft (5) is controlled to slide forwards horizontally, the spline (52) of the rotating shaft (5) slides out of the spline groove (42), the worm (53) moves forwards horizontally, in the process that the worm (53) moves forwards horizontally, the first gear (75) is driven by the worm (53) to rotate through the first gear (75), the third driving wheel (72) above the first gear (75) is controlled to rotate through the second driving wheel (71) under the driving action of the second belt (73), the second gear (75) above the first rotating rod is caused to rotate, and after the connecting plate (31) rises to the highest point, the toothed ring (38) is meshed with the second gear (75), so that the movable frame (36) is overturned, and the upper part of the culture dish is leaked;

s5: the worm (53) is meshed with the worm wheel (84) after passing through the first gear (74), and along with the rotation of the rotating shaft (5), the second rotating rod (8) is controlled to rotate, the motor rotates positively and negatively, so that the lifting column (93) drives the lifting seat (9) to move up and down, and the up and down position of the microscope (92) is controlled, so that whether mycoplasma is generated in the culture medium is observed;

s6: the motor is reversely started, so that the lifting seat (9) is controlled to ascend and reset by the rotation of the screw rod (85), the rotating shaft (5) is controlled to shrink backwards through the oil cylinder, the first gear (74) is driven to reversely rotate in the process, the second gear (75) is used for controlling the movable frame (36) to reversely rotate 180 degrees, the movable frame is reset, the rotating shaft (5) is continuously controlled to shrink backwards until the spline (52) is clamped into the spline groove (42), so that culture dishes on the other movable plates (31) are moved to the highest point, and the mycoplasma culture condition in each culture dish is observed.