CN112572997A - Temperature-adjusting preservation box for temporarily preserving in-vitro biological samples and use method - Google Patents

Abstract

The invention relates to a temperature-adjusting preservation box for temporarily preserving an in-vitro biological sample and a using method thereof, belongs to the technical field of in-vitro biological sample preservation devices, and solves the technical problems that in-vitro living tissues are simple and crude in preservation and preservation liquid in the same preservation box is single in component. The solution is as follows: the device comprises a box body, a storage device, a driving system and a lifting platform, wherein a box body interlayer is arranged on the side wall of the box body, the driving system is arranged in the box body interlayer, the lifting platform is arranged in the box body, the driving system drives the lifting platform to reciprocate up and down in the box body, the storage device is arranged above the lifting platform, and a box cover is arranged above the box body. According to the invention, the preservation tubes are independently arranged in the same preservation box, and the preservation tubes are respectively filled with preservation liquids with different components according to needs, so that different types of temporary preservation of the same in-vitro biological sample can be realized in the same preservation box, and the file arrangement and transportation in the inspection process are facilitated; meanwhile, the temperature can be adjusted, and proper preservation solution is selected, so that an environment similar to the in vivo environment is provided as far as possible, and the activity of the in vitro living tissue is not influenced as far as possible.

Description

Temperature-adjusting preservation box for temporarily preserving in-vitro biological samples and use method

Technical Field

The invention belongs to the technical field of isolated biological sample preservation devices, and particularly relates to a temperature-adjusting preservation box for temporarily preserving an isolated biological sample and a using method thereof.

Background

The focus of a patient can be effectively analyzed by collecting the in-vitro biological sample, and in general, in order to avoid the pain caused by repeated sampling of the patient, the collected in-vitro biological sample needs to be subjected to different types of detection and analysis as much as possible, namely, the in-vitro biological sample usually needs to be temporarily stored for many times, so that all projects to be detected are completed. In the prior art, the preservation solution stored in one preservation box is often the same component, however, the preservation form of the same isolated biological sample may be different according to the different requirements of the project to be detected, which causes the isolated biological sample to be preserved in different preservation boxes, and brings inconvenience for the archive arrangement and transportation in the inspection process.

Secondly, in the student experiments of medical colleges and universities, a plurality of experiments for observing the physiological activities of certain tissues and organs exist, usually, the isolated tissues and organs are only simply stored in physiological saline, and the physiological activities of the isolated tissues and organs are greatly influenced due to the large experimental time span, so that the experimental results are influenced, and an environment similar to the in-vivo environment needs to be provided as far as possible to ensure that the activities of the isolated tissues and organs are not influenced as far as possible.

Disclosure of Invention

In order to overcome the defects of the prior art and solve the technical problems of simple and crude living tissue temporary preservation environment and single preservation solution component in the same preservation box, the invention provides a temperature-regulating preservation box for temporarily preserving an in-vitro biological sample and a using method thereof.

The invention is realized by the following technical scheme.

The utility model provides a case that preserves adjusts temperature for biological sample temporary preservation in vitro, it includes box, save set, actuating system and lift platform, wherein: the side wall of the box body is provided with a box body interlayer, the driving system is arranged in the box body interlayer, the lifting platform is arranged in the box body, the driving system drives the lifting platform to reciprocate up and down in the box body, the storage device is arranged above the lifting platform, and a box cover is covered above the box body;

the driving system comprises a motor, a driving gear, a driven gear, a transmission bevel gear set, a driving shaft, a transmission shaft and a driven shaft, wherein the motor is arranged on the rear side wall of the box body, the motor is fixedly connected with the driving shaft through a coupler, the driving shaft extends into a box body interlayer on the side wall of the box body, the transmission shaft is arranged in the box body interlayer on the rear side wall of the box body, the driven shaft is arranged in the box body interlayer on the side wall of the box body opposite to the driving shaft, the driving gear is arranged on the driving shaft, the driving shaft drives the transmission shaft to rotate through a first transmission bevel gear set, the transmission;

racks are respectively arranged on the left side and the right side of the lifting platform along the vertical direction, sliding grooves are formed in positions, corresponding to the racks, on the inner wall of the box body, driving gears and driven gears are respectively meshed with the corresponding racks, and the driving gears and the driven gears synchronously drive the corresponding racks to reciprocate up and down along the sliding grooves;

the storage device comprises a storage groove, a storage tube and a sample taking and placing rod, the storage groove is placed above the lifting platform, a plurality of clamping bases are arranged at the bottom of the storage groove, and a micro refrigerating device and a heating device are respectively arranged on the bottom surface of the storage groove at the gaps of the clamping bases; the preservation pipes are vertically upwards detachably clamped in the corresponding clamping bases, and liquid inlet hoses for filling preservation liquid into the preservation pipes are arranged at the bottoms of the side walls of the preservation pipes; the sample taking and placing rod extends to the bottom of the storage tube from top to bottom, a tray for temporarily storing samples is arranged at the lower end of the sample taking and placing rod, and a limiting baffle is arranged at the upper end of the sample taking and placing rod;

through holes are respectively arranged at positions, corresponding to the positions of the preservation pipes, on the box cover, a sample taking and placing door is hinged above the through holes, a through groove is formed in the sample taking and placing door along the radius direction, sealing strips are oppositely arranged in the through grooves, a sample taking and placing rod is clamped in the through grooves, and a limiting baffle is clamped above the sample taking and placing door; the lower surface of the box cover is provided with a quick connector assembly, and the liquid inlet hoses are respectively and detachably communicated with liquid outlets of the quick connector assembly; be provided with the case lid intermediate layer in the case lid, be provided with a plurality of preservation cistern that are used for holding different composition preserving liquids in the case lid intermediate layer, the liquid outlet of preserving the cistern respectively with quick interface assembly's inlet intercommunication, preserve and install the liquid feed pump on the pipeline of cistern and quick interface assembly intercommunication respectively.

Furthermore, a plurality of storage tubes with different tube diameters are arranged according to the size of a sample to be stored, and the clamping base is correspondingly arranged according to the tube diameter of each storage tube.

Furthermore, the storage tubes are sequentially arranged in the storage tank from large to small according to the tube diameter.

Further, the sample taking and placing doors are respectively marked with storage numbers.

A use method of a temperature-adjusting preservation box for temporarily preserving an in vitro biological sample comprises the following steps:

s1, according to the requirement of in vitro biological sample to be detected, the in vitro biological sample is cut into a plurality of small pieces, a storage tube with a proper diameter is selected according to the size of the in vitro biological sample, a box cover is opened, the lifting platform is located at the end of the lower stroke, the storage tube is vertically and upwards clamped in a corresponding clamping base, a liquid inlet hose is communicated with the quick connector assembly, the box cover is buckled above the box body, and the sample taking and placing door is naturally closed;

s2, the motor drives the driving wheel and the driven wheel to synchronously rotate through the driving system, the driving wheel and the driven wheel drive the corresponding racks to move upwards to the upper stroke end position along the sliding groove, so that the lifting platform drives the storage groove to move to the upper stroke end position, and in the process of the upward movement of the storage groove, the storage tube jacks the corresponding sample taking and placing door on the box cover to open, so that the storage tube extends to the outside of the box body;

s3, placing the in-vitro biological sample to be temporarily stored into a tray, extending a handheld sample taking and placing rod to the bottom of a storage tube from top to bottom, then reversely rotating a motor, driving a lifting platform to move to a lower stroke end position through a driving system, naturally closing a sample taking and placing door in the process that the storage tube descends along with a storage groove, adjusting the position of the sample taking and placing rod to enable the sample taking and placing rod to penetrate through a sealing strip and be clamped in a through groove, and adjusting the position of a limiting baffle to enable the sample taking and placing rod to be clamped above the sample taking and placing door;

s4, a liquid supply pump is arranged on a pipeline communicated with the quick connector assembly by controlling a preservation liquid groove, and preservation liquid of specific components is added into a preservation pipe through the preservation liquid groove, the quick connector assembly and a liquid inlet hose; adjusting the temperature of the micro refrigerating device or the heating device to enable the required temperature to be formed in the storage tank, temporarily storing the in-vitro biological sample in the storage tank, and simultaneously recording the storage number of the in-vitro biological sample;

s5, repeating the step S2, taking out the isolated biological sample temporarily stored in any storage tube through the sample taking and placing rod according to the requirement, and finishing the temporary storage of the isolated biological sample in any storage tube;

s6, repeating the steps S3-S5 until all in-vitro biological samples in the box body are taken out, and stopping the operation of the micro refrigerating device or the heating device;

s7, taking down the box cover above the box body, disconnecting the liquid inlet hose from the quick connector assembly, taking out the storage tube from the clamping base, pouring out waste liquid in the storage tube, cleaning the storage tube, and moving the lifting platform to the final position of the lower stroke again to wait for next use.

Compared with the prior art, the invention has the beneficial effects that:

according to the invention, the preservation tubes are independently arranged in the same preservation box, and the preservation liquids with different components are respectively added into the preservation tubes according to needs, so that different types of temporary preservation of the same isolated biological sample can be realized in the same preservation box, and the file arrangement and transportation in the inspection process are facilitated.

Drawings

FIG. 1 is a schematic sectional view of the present invention in a front view;

FIG. 2 is a schematic sectional view of the elevating platform in a raised state;

FIG. 3 is a front sectional structural view of the elevator platform in a lowered position;

FIG. 4 is a schematic top view of the present invention without the cover;

fig. 5 is a schematic top view of the sample access door.

In the figure, 1 is a box body, 11 is a box body interlayer, 12 is a sliding chute, 2 is a storage device, 21 is a storage tank, 22 is a storage pipe, 23 is a sample taking and placing rod, 24 is a clamping base, 25 is a micro refrigerating device, 26 is a heating device, 27 is a liquid inlet hose, 28 is a tray, 29 is a limit baffle, 3 is a lifting platform, 31 is a rack, 4 is a box cover, 41 is a through hole, 42 is a sample taking and placing door, 43 is a through groove, 44 is a sealing strip, 45 is a quick connector assembly, 46 is a box cover interlayer, 47 is a storage liquid tank, 51 is a motor, 52 is a driving gear, 53 is a driven gear, 54 is a driving shaft, 55 is a transmission shaft, 56 is a driven shaft, 57 is a first transmission bevel gear set, and 58 is a second transmission bevel gear set.

Detailed Description

The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention. Unless otherwise specified, the examples follow conventional experimental conditions. In addition, it will be apparent to those skilled in the art that various modifications or improvements can be made to the material components and amounts in these embodiments without departing from the spirit and scope of the invention as defined in the appended claims.

Fig. 1 to 5 show a temperature-controlled storage for temporary storage of in vitro biological samples, which comprises a box body 1, a storage device 2, a driving system and a lifting platform 3, wherein: a box body interlayer 11 is arranged on the side wall of the box body 1, a driving system is arranged in the box body interlayer 11, a lifting platform 3 is arranged in the box body 1, the driving system drives the lifting platform 3 to reciprocate up and down in the box body 1, a storage device 2 is arranged above the lifting platform 3, and a box cover 4 is covered above the box body 1;

the driving system comprises a motor 51, a driving gear 52, a driven gear 53, a transmission bevel gear set, a driving shaft 54, a transmission shaft 55 and a driven shaft 56, wherein the motor 51 is arranged on the rear side wall of the box body 1, the motor 51 is fixedly connected with the driving shaft 54 through a coupler, the driving shaft 54 extends into a box body interlayer 11 on the side wall of the box body 1, the transmission shaft 55 is arranged in the box body interlayer 11 on the rear side wall of the box body 1, the driven shaft 56 is arranged in the box body interlayer 11 on the side wall of the box body 1 opposite to the driving shaft 54, the driving gear 52 is arranged on the driving shaft 54, the driving shaft 54 drives the transmission shaft 55 to rotate through a first transmission bevel gear set 57, the transmission shaft 55 drives the;

racks 31 are respectively arranged on the left side and the right side of the lifting platform 3 along the vertical direction, sliding grooves 12 are arranged on the inner wall of the box body 1 corresponding to the racks 31, driving gears 52 and driven gears 53 are respectively meshed with the corresponding racks 31, and the driving gears 52 and the driven gears 53 synchronously drive the corresponding racks 31 to reciprocate up and down along the sliding grooves 12;

the storage device 2 comprises a storage groove 21, a storage tube 22 and a sample taking and placing rod 23, the storage groove 21 is placed above the lifting platform 3, a plurality of clamping bases 24 are arranged at the bottom of the storage groove 21, and a micro refrigerating device 25 and a heating device 26 are respectively arranged at the gaps of the clamping bases 24 on the bottom surface of the storage groove 21; the preservation pipes 22 are vertically and upwards detachably clamped in the corresponding clamping bases 24, and liquid inlet hoses 27 used for filling preservation liquid into the preservation pipes 22 are arranged at the bottoms of the side walls of the preservation pipes 22; the sample taking and placing rod 23 extends to the bottom of the storage tube 22 from top to bottom, a tray 28 for temporarily storing samples is arranged at the lower end of the sample taking and placing rod 23, and a limit baffle 29 is arranged at the upper end of the sample taking and placing rod 23;

through holes 41 are respectively arranged at the positions, corresponding to the positions of the preservation pipes 22, on the box cover 4, a sample taking and placing door 42 is hinged above the through holes 41, a through groove 43 is arranged on the sample taking and placing door 42 along the radius direction, sealing strips 44 are oppositely arranged in the through groove 43, a sample taking and placing rod 42 is clamped in the through groove 43, and a limit baffle 29 is clamped above the sample taking and placing door 42; a quick connector assembly 45 is arranged on the lower surface of the box cover 4, and the liquid inlet hoses 27 are respectively and detachably communicated with liquid outlets of the quick connector assembly 45; be provided with case lid intermediate layer 46 in the case lid 4, be provided with a plurality of preservation cistern 47 that are used for holding different composition preservation liquid in the case lid intermediate layer 46, the liquid outlet of preserving cistern 47 communicates with the inlet of quick interface assembly 45 respectively, preserves the installation liquid feed pump on the pipeline of cistern 47 and quick interface assembly 45 intercommunication respectively.

Furthermore, a plurality of storage tubes 22 with different tube diameters are arranged according to the size of the sample to be stored, and the clamping base 24 is correspondingly arranged according to the tube diameter of the storage tubes 22.

Further, the storage tubes 22 are arranged in the storage tank 21 in order of the tube diameter from the larger to the smaller.

Further, the sample taking and placing doors 42 are respectively marked with storage numbers.

The using process of the invention is as follows:

s1, according to the requirement of the isolated biological sample to be detected, the isolated biological sample is cut into a plurality of small pieces, a storage tube 22 with a proper diameter is selected according to the size of the isolated biological sample, the box cover 4 is opened, the lifting platform 3 is at the end of the lower stroke, the storage tube 22 is vertically clamped upwards in the corresponding clamping base 24, the box cover 4 is buckled above the box body 1 after the liquid inlet hose 27 is communicated with the quick connector assembly 45, and the sample taking and placing door 42 is naturally closed;

s2, the motor 51 drives the driving wheel 52 and the driven wheel 53 to synchronously rotate through the driving system, the driving wheel 52 and the driven wheel 53 drive the corresponding rack 31 to move upwards to the upper stroke end position along the sliding chute 12, so that the lifting platform 3 drives the storage tank 21 to move to the upper stroke end position, and in the process that the storage tank 21 moves upwards, the storage tube 22 jacks the corresponding sample taking and placing door 42 on the box cover 4, so that the storage tube 22 extends to the outside of the box body;

s3, placing the in-vitro biological sample to be temporarily stored in the tray 28, holding the sample taking and placing rod 23 by hand to extend to the bottom of the storage tube 22 from top to bottom, then reversing the motor 51, driving the lifting platform 3 by the motor 51 through the driving system to move to the final lower stroke position, naturally closing the sample taking and placing door 42 in the process that the storage tube 22 descends along with the storage groove 21, adjusting the position of the sample taking and placing rod 42 to enable the sample taking and placing rod to penetrate through the sealing strip to be clamped in the through groove 43, and adjusting the position of the limit baffle 29 to enable the sample taking and placing rod to be clamped above the sample taking and placing door 42;

s4, a liquid supply pump is arranged on a pipeline communicated with the quick connector assembly 45 by controlling the preservation liquid tank 47, and preservation liquid of specific components is added into the preservation pipe 22 through the preservation liquid tank 47, the quick connector assembly 45 and the liquid inlet hose 27; adjusting the temperature of the micro-refrigerating device 25 or the heating device 26 to form the required temperature in the preservation tank 21, temporarily preserving the in vitro biological sample in the preservation tank, and simultaneously recording the storage number of the in vitro biological sample;

the micro-refrigeration device 25 and the heating device 26 are both sold in the market, for example, the micro-refrigeration device 25 adopts a Chinese patent 'a micro-refrigeration device' (publication number: CN 111322779A), and the heating device 26 adopts a Chinese patent 'a micro-heater' (publication number: CN 101751052B);

s5, repeating the step S2, taking out the isolated biological sample temporarily stored in any storage tube 22 through the sample taking and placing rod 23 according to the requirement, and finishing the temporary storage of the isolated biological sample in any storage tube 22;

s6, repeating the steps S3-S5 until all in-vitro biological samples in the box body 1 are taken out, and stopping the operation of the micro refrigerating device 25 or the heating device 26;

s7, taking down the box cover 4 above the box body, disconnecting the liquid inlet hose 27 from the quick connector assembly 45, taking out the storage tube 22 from the clamping base 24, pouring out waste liquid in the storage tube 22, cleaning the storage tube 22, and moving the lifting platform 3 to a lower stroke end position again to wait for next use.

The above description is only for the specific embodiments of the present invention, but the scope of the present invention is not limited thereto, and any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope of the present invention are included in the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the appended claims.

Claims (5)

1. The utility model provides a hutch that adjusts temperature for biological sample of separation preserves temporarily, it includes box (1), save set (2), actuating system and lift platform (3), its characterized in that: a box body interlayer (11) is arranged on the side wall of the box body (1), a driving system is arranged in the box body interlayer (11), a lifting platform (3) is arranged in the box body (1), the driving system drives the lifting platform (3) to reciprocate up and down in the box body (1), a storage device (2) is arranged above the lifting platform (3), and a box cover (4) is arranged above the box body (1) in a covering manner;

the driving system comprises a motor (51), a driving gear (52), a driven gear (53), a transmission bevel gear set, a driving shaft (54), a transmission shaft (55) and a driven shaft (56), wherein the motor (51) is arranged on the rear side wall of the box body (1), the motor (51) is fixedly connected with the driving shaft (54) through a coupler, the driving shaft (54) extends into a box body interlayer (11) on the side wall of the box body (1), the transmission shaft (55) is arranged in the box body interlayer (11) on the rear side wall of the box body (1), the driven shaft (56) is arranged in the box body interlayer (11) on the side wall of the box body (1) on the opposite side of the driving shaft (54), the driving gear (52) is arranged on the driving shaft (54), the driving shaft (54) drives the transmission shaft (55) to rotate through a transmission bevel gear set I (57), and the transmission shaft (55, a driven gear (53) is arranged on the driven shaft (56);

racks (31) are respectively arranged on the left side and the right side of the lifting platform (3) along the vertical direction, sliding grooves (12) are formed in positions, corresponding to the racks (31), on the inner wall of the box body (1), driving gears (52) and driven gears (53) are respectively meshed with the corresponding racks (31), and the driving gears (52) and the driven gears (53) synchronously drive the corresponding racks (31) to reciprocate up and down along the sliding grooves (12);

the storage device (2) comprises a storage groove (21), a storage tube (22) and a sample taking and placing rod (23), the storage groove (21) is placed above the lifting platform (3), a plurality of clamping bases (24) are arranged at the bottom of the storage groove (21), and a miniature refrigerating device (25) and a heating device (26) are respectively arranged on the bottom surface of the storage groove (21) and in the gaps of the clamping bases (24); the preservation pipes (22) are vertically upwards detachably clamped in the corresponding clamping bases (24), and liquid inlet hoses (27) used for filling preservation liquid into the preservation pipes (22) are arranged at the bottoms of the side walls of the preservation pipes (22); the sample taking and placing rod (23) extends to the bottom of the storage tube (22) from top to bottom, a tray (28) for temporarily storing samples is arranged at the lower end of the sample taking and placing rod (23), and a limit baffle (29) is arranged at the upper end of the sample taking and placing rod (23);

through holes (41) are respectively arranged at positions, corresponding to the positions of the preservation pipes (22), on the box cover (4), sample taking and placing doors (42) are hinged above the through holes (41), through grooves (43) are formed in the sample taking and placing doors (42) along the radius direction, sealing strips (44) are oppositely arranged in the through grooves (43), sample taking and placing rods (42) are clamped in the through grooves (43), and limit baffles (29) are clamped above the sample taking and placing doors (42); a quick connector assembly (45) is arranged on the lower surface of the box cover (4), and the liquid inlet hose (27) is respectively and detachably communicated with a liquid outlet of the quick connector assembly (45); be provided with case lid intermediate layer (46) in case lid (4), be provided with a plurality of preservation cistern (47) that are used for holding different composition preservation liquid in case lid intermediate layer (46), the liquid outlet of preserving cistern (47) communicates with the inlet of quick interface assembly (45) respectively, installs the liquid feed pump on the pipeline of preserving cistern (47) and quick interface assembly (45) intercommunication respectively.

2. The storage case of claim 1, wherein the temperature-controlled storage case is used for temporary storage of in vitro biological samples, and comprises: a plurality of preserving tubes (22) with different tube diameters are arranged according to the size of a sample to be preserved, and the size of the clamping base (24) is correspondingly arranged according to the tube diameter of the preserving tubes (22).

3. The storage case of claim 2, wherein the temperature-controlled storage case is used for temporary storage of in vitro biological samples, and comprises: the storage tubes (22) are sequentially arranged in the storage tank (21) from large to small according to the tube diameter.

4. The storage case of claim 1, wherein the temperature-controlled storage case is used for temporary storage of in vitro biological samples, and comprises: the sample taking and placing doors (42) are respectively marked with storage numbers.

5. The method of claim 1, wherein the method comprises the steps of:

s1, according to the requirement of an isolated biological sample to be detected, the isolated biological sample is cut into a plurality of small pieces, a storage tube (22) with a proper pipe diameter is selected according to the size of the isolated biological sample, a box cover (4) is opened, at the moment, a lifting platform (3) is located at the end of the lower stroke, the storage tube (22) is vertically and upwards clamped in a corresponding clamping base (24), a liquid inlet hose (27) is communicated with a quick connector assembly (45), the box cover (4) is buckled above a box body (1), and a sample taking and placing door (42) is naturally closed;

s2, a motor (51) drives a driving wheel (52) and a driven wheel (53) to synchronously rotate through a driving system, the driving wheel (52) and the driven wheel (53) drive corresponding racks (31) to move upwards to an upper stroke end position along a sliding groove (12), so that a lifting platform (3) drives a storage groove (21) to move to the upper stroke end position, and in the process of upward movement of the storage groove (21), a storage tube (22) jacks a corresponding sample taking and placing door (42) on a box cover (4) open, so that the storage tube (22) extends to the outside of the box body;

s3, putting the in-vitro biological sample to be temporarily stored into a tray (28), holding a sample taking and placing rod (23) by hand, extending to the bottom of a storage tube (22) from top to bottom, then reversing a motor (51), driving a lifting platform (3) by the motor (51) through a driving system to move to a lower stroke end position, naturally closing a sample taking and placing door (42) in the process that the storage tube (22) descends along with a storage groove (21), adjusting the position of the sample taking and placing rod (42) to enable the sample taking and placing rod to penetrate through a sealing strip to be clamped into a through groove (43), and adjusting the position of a limiting baffle (29) to enable the sample taking and placing rod to be clamped above the sample taking and placing door (42);

s4, a liquid supply pump is arranged on a pipeline communicated with the quick connector assembly (45) by controlling the preservation liquid tank (47), and preservation liquid of specific components is added into the preservation pipe (22) through the preservation liquid tank (47), the quick connector assembly (45) and the liquid inlet hose (27); adjusting the temperature of a micro refrigerating device (25) or a heating device (26) to enable the required temperature to be formed in the preservation tank (21), temporarily preserving the in vitro biological sample in the preservation tank, and simultaneously recording the storage number of the in vitro biological sample;

s5, repeating the step S2, taking out the isolated biological sample temporarily stored in any storage tube (22) through the sample taking and placing rod (23) according to the requirement, and finishing the temporary storage of the isolated biological sample in any storage tube (22);

s6, repeating the steps S3-S5 until all in-vitro biological samples in the box body (1) are taken out, and stopping the operation of the micro refrigerating device (25) or the heating device (26);

s7, taking down the box cover (4) above the box body, disconnecting the liquid inlet hose (27) from the quick connector assembly (45), taking out the storage tube (22) from the clamping base (24), pouring out waste liquid in the storage tube (22), cleaning the storage tube (22), and moving the lifting platform (3) to a lower stroke end position again to wait for next use.

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