CN117417815B - Bottle-shaped culture medium device - Google Patents
Bottle-shaped culture medium device Download PDFInfo
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- CN117417815B CN117417815B CN202311743270.7A CN202311743270A CN117417815B CN 117417815 B CN117417815 B CN 117417815B CN 202311743270 A CN202311743270 A CN 202311743270A CN 117417815 B CN117417815 B CN 117417815B
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- 239000001963 growth medium Substances 0.000 title claims abstract description 55
- 238000007789 sealing Methods 0.000 claims abstract description 80
- 239000011159 matrix material Substances 0.000 claims abstract description 31
- 239000007787 solid Substances 0.000 claims abstract description 30
- 239000012528 membrane Substances 0.000 claims abstract description 21
- 238000001914 filtration Methods 0.000 claims abstract description 16
- 239000000758 substrate Substances 0.000 claims abstract description 16
- 238000009630 liquid culture Methods 0.000 claims abstract description 15
- 238000003825 pressing Methods 0.000 claims description 18
- 239000002609 medium Substances 0.000 claims description 12
- 210000001503 joint Anatomy 0.000 claims description 9
- 230000000903 blocking effect Effects 0.000 claims description 8
- 239000002390 adhesive tape Substances 0.000 claims description 4
- 230000007423 decrease Effects 0.000 claims description 3
- 230000002093 peripheral effect Effects 0.000 claims 1
- 239000007788 liquid Substances 0.000 abstract description 12
- 239000013049 sediment Substances 0.000 abstract description 4
- 230000002906 microbiologic effect Effects 0.000 abstract description 2
- 238000000926 separation method Methods 0.000 abstract description 2
- 230000009286 beneficial effect Effects 0.000 description 8
- 238000009434 installation Methods 0.000 description 4
- 239000000853 adhesive Substances 0.000 description 3
- 230000001070 adhesive effect Effects 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 1
- 235000017491 Bambusa tulda Nutrition 0.000 description 1
- 241001330002 Bambuseae Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 235000015334 Phyllostachys viridis Nutrition 0.000 description 1
- 238000004026 adhesive bonding Methods 0.000 description 1
- 210000004102 animal cell Anatomy 0.000 description 1
- 239000011425 bamboo Substances 0.000 description 1
- 238000005842 biochemical reaction Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000002360 explosive Substances 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
- 238000005057 refrigeration Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- -1 trace elements) Chemical class 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/46—Means for fastening
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/50—Means for positioning or orientating the apparatus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/04—Seals
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Molecular Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention belongs to the technical field of bottle-shaped microbiological devices, and relates to a bottle-shaped culture medium device, which comprises a bottle-shaped substrate, an inner support and a bottle cap, wherein the bottle-shaped substrate is used for storing a liquid culture medium, and the inner support is used for storing a solid culture medium; the bottle cap is internally and coaxially connected with a positioning cylinder and a rod body, the rod body is positioned in the positioning cylinder, and the lower end of the rod body extends out of the bottle cap; the inner support comprises a storage cylinder body and a circle of flange which is used for being in stop fit with the bottle-shaped base body, the storage cylinder body is used for being inserted into the bottle-shaped base body, a detachable baffle is arranged on the inner bottom wall of the storage cylinder body, a filtering membrane cylinder is sleeved at the bottom of the storage cylinder body, and a filtering membrane is arranged in the filtering membrane cylinder; the upper end of the bottle-shaped matrix is detachably connected with a limiting component for limiting the bottle cap, and a sealing component is arranged between the bottle-shaped matrix and the bottle cap. The invention can realize the separation of solid and liquid, realize the storage at normal temperature in dark place through sealing, can be quickly disassembled and assembled, is convenient and quick to use, and is not easy to generate sediment.
Description
Technical Field
The invention belongs to the technical field of bottle-shaped microbiological devices, and particularly relates to a bottle-shaped culture medium device.
Background
The culture medium refers to a nutrient medium which is used for the growth and reproduction of microorganisms, plants or animals (or tissues) and is prepared by combining different nutrient substances. Generally contains carbohydrate, nitrogenous substances, inorganic salts (including trace elements), vitamins, water and other substances. The culture medium may be classified into a basic culture medium, an enrichment culture medium, a selection culture medium, a differential culture medium, and an anaerobic culture medium according to its composition and use, and the use of the culture medium mainly includes: growth and reproduction of living organisms such as microorganisms, plant or animal cells; for the identification, classification and enumeration of microorganisms; storing the strain or maintaining the activity of the strain; scientific researches, such as exploring physiological and biochemical reaction mechanisms in life process, have been conducted, and a liquid culture medium is a culture medium in liquid form, which is a solution composed of water and various nutrients. Compared with a solid culture medium, the liquid culture medium is more convenient to use, can provide larger culture capacity, can better control culture conditions, has high requirements on the storage conditions of the existing liquid culture medium, cannot separate solids and liquids in the culture medium, can be transported only by storing the solids and the liquids in a refrigeration house, has shorter shelf life, is inconvenient to store the solids and the liquids, and can generate sediment to influence the use of subsequent staff.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides the bottle-shaped culture medium device which can separate solids and liquid, can realize light-proof normal-temperature storage through multistage sealing, can be quickly disassembled and assembled, is convenient and quick to use and is not easy to generate sediment.
In order to solve the technical problems, the invention adopts the following technical scheme:
a bottle-shaped culture medium device comprises a bottle-shaped matrix, an inner support and a bottle cap, wherein the bottle-shaped matrix is used for storing a liquid culture medium, and the inner support is used for storing a solid culture medium; the bottle cap is in threaded connection with the bottle-shaped matrix, a positioning cylinder and a rod body are coaxially connected in the bottle cap, the rod body is positioned in the positioning cylinder, and the lower end of the rod body extends out of the bottle cap; the inner support comprises a storage cylinder body and a circle of flange which is connected to the top of the storage cylinder body and is used for being in stop fit with the bottle-shaped substrate, the storage cylinder body is used for being inserted into the bottle-shaped substrate, a detachable baffle is arranged on the inner bottom wall of the storage cylinder body and is used for preventing a solid culture medium from entering the bottle-shaped substrate, a filtering membrane cylinder is sleeved at the bottom of the storage cylinder body, and a filtering membrane positioned below the baffle is arranged in the filtering membrane cylinder; the upper end of the bottle-shaped matrix is detachably connected with a limiting assembly for limiting the bottle cap, and a sealing assembly is arranged between the bottle-shaped matrix and the bottle cap; after the limiting component is removed, the bottle cap is pressed downwards, so that the rod body can eject the baffle plate from the storage cylinder body to enable the solid culture medium in the inner support to be mixed with the liquid culture medium in the bottle-shaped matrix; and after the limiting component is removed, the bottle cap is pressed downwards, so that the positioning cylinder can be inserted into the storage cylinder and connected with the storage cylinder.
Compared with the prior art, the invention has the beneficial effects that: according to the invention, the liquid culture medium is stored in the bottle-shaped matrix, and the solid culture medium is stored in the inner support, so that the separation of solids and liquid can be realized, and the light-shielding normal-temperature storage can be realized. When the bottle cap is used, the limit of the limit component on the bottle cap is removed, the bottle cap is screwed downwards, the rod body can break the baffle plate, and the solid culture medium in the inner support can be contacted with and fused with the solution in the bottle-shaped matrix through the through hole at the baffle plate, so that the bottle cap is very convenient to use; in addition, the filtering membrane in the filtering membrane cylinder can stop the baffle plate poked down by the rod body and filter impurity solids mistakenly entering the inner support, so that the solution is prevented from being polluted, and sediment is not easy to generate.
As a further improvement of the invention, the inner diameter of the storage cylinder body gradually decreases from top to bottom, and the outer diameter of the positioning cylinder is smaller than the maximum inner diameter of the storage cylinder body and larger than the minimum inner diameter of the storage cylinder body, so that the positioning cylinder can be in interference fit with the storage cylinder body after being inserted into the storage cylinder body.
The beneficial effects are that: after the solid culture medium and the liquid culture medium are mixed, the bottle cap can be matched with the inner support and taken out of the bottle body together.
As a further improvement of the invention, the bottom of the bottle-shaped base body is provided with a base, the upper end of the base is provided with a clamping cylinder, the inside of the clamping cylinder is connected with a placing disc in a sliding way along the up-down direction, the placing disc is used for placing the bottle-shaped base body, and the inside of the clamping cylinder is provided with a fixing component for fixing the bottle-shaped base body; the outer arc circumferential surface of the bottle-shaped matrix is provided with scale marks which are arranged at equal intervals along the up-down direction.
The beneficial effects are that: is favorable for realizing the stable placement of the bottle-shaped matrix and can observe the capacity of the solution in the bottle-shaped matrix.
As a further improvement of the invention, the fixing component comprises two clamping groove frames arranged on the inner arc wall of the clamping cylinder, the left and right positions of the two clamping groove frames correspond to each other, two clamping blocks are arranged at the lower end of the outer arc circumferential surface of the bottle-shaped base body and are used for being clamped into the corresponding clamping groove frames, mounting springs with two ends respectively connected with the placing disc and the base are arranged in the clamping cylinder, and the mounting springs are used for pushing the bottle-shaped base body to enable the clamping blocks to be in blocking fit with the clamping groove frames upwards.
The beneficial effects are that: through aiming at the bottle type base member and placing the dish and pressing downwards for place the dish and move downwards, the installation spring compression this moment, then the rotatory bottle type base member of personnel makes the joint piece rotatory to the inside of draw-in groove frame, loosens the hand afterwards, and the quick resilience of installation spring drives the bottle type base member on placing the dish this moment and upwards moves, makes joint piece and draw-in groove frame offset, thereby realizes the fixed to the bottle type base member, can prevent that the bottle type base member from being touched the influence personnel and use, also makes things convenient for personnel to get at any time and puts the bottle type base member, makes personnel use more conveniently.
As a further improvement of the invention, the limiting component comprises a detachable pull ring which surrounds the periphery of the bottle-shaped base body, and the detachable pull ring is matched with the bottle cap in a blocking way in the up-down direction;
or the limiting component comprises a cavity ring arranged at the upper end of the bottle-shaped matrix, and two locking frames are respectively connected in the cavity ring in a sliding manner through two inserted posts; the two inserted columns are respectively positioned at the front side and the rear side of the radial direction of the bottle-shaped matrix, and one end of each inserted column penetrates out of the cavity ring and is connected with a blocking head which is used for being blocked and matched with the cavity ring; the outer arc circumferential surface of the bottle cap is provided with a jack corresponding to each inserted column for being in plug-in fit with the inserted column, a reset spring is connected between the cavity ring and each locking frame, and the reset spring is sleeved outside the corresponding inserted column; the two ends of each locking frame are respectively and rotatably connected with a connecting rod, and two connecting rods positioned on the same locking frame are symmetrical relative to a reset spring connected with the locking frame; two adjusting seats are respectively connected in the cavity ring in a sliding way through two sliding columns, the two adjusting seats are respectively positioned at the radial left side and the radial right side of the bottle-shaped base body, and one end of each sliding column extends out of the cavity ring and is connected with a pressing disc; one end, far away from the locking frame, of two connecting rods which are rotationally connected on the same locking frame is rotationally connected with corresponding ends of two adjusting seats respectively.
The beneficial effects are that: the limit of the bottle cap can be relieved directly by tearing off the pull ring; the push plate on both sides can also be pressed, the adjusting seat on both sides is driven to be close to each other through the sliding column, so that the connecting rod inside the adjusting seat rotates, and then the locking frame around the driving is far away from each other under the limit of the inserting column, so that the inserting column leaves the inside of the jack, then a person upwards takes down the bottle cap from the bottle-shaped matrix, and the bottle cap and the bottle-shaped matrix can be quickly detached, so that the use of the person is more convenient.
As a further improvement of the invention, a plurality of first anti-slip protrusions are arranged on the outer arc circumferential surface of the bottle cap, and a plurality of second anti-slip protrusions are arranged on the pressing surface of the pressing plate.
The beneficial effects are that: the first anti-slip protrusion on the bottle cover and the second anti-slip protrusion on the pressing disc can prevent people from slipping by hand, and are beneficial to operation.
As a further improvement of the invention, the sealing component comprises a rectangular sealing ring adhered to the upper end of the bottle-shaped substrate, the upper end of the intrados of the bottle cap is provided with a rectangular sealing groove, and the rectangular sealing ring is matched with the rectangular sealing groove; the lower end of the bottle-shaped substrate is adhered with a trapezoid sealing ring, the lower end of the inner cambered surface of the bottle cap is provided with a trapezoid sealing groove, and the trapezoid sealing ring is matched with the trapezoid sealing groove; the upper end of bottle base member bonds there is the arc sealing washer that is located between rectangle sealing washer and the trapezoidal sealing washer, the arc seal groove has been seted up on the intrados of bottle lid, arc sealing washer and arc seal groove cooperation installation, be provided with the bonding ring on the roof of bottle lid, bonding ring and interior support cooperation installation, the lower extreme of bottle lid is provided with auxiliary sealing unit.
The beneficial effects are that: can effectively prevent that solid medium from leaking the problem through gluing circle and collet contact, rectangular seal circle and rectangular seal groove contact can prevent that liquid medium from overflowing the problem from appearing simultaneously, realize first layer of sealedly, arc seal circle groove extrudees to the inside second time of formation of arc seal groove and seals, and trapezoidal seal circle and trapezoidal seal groove cooperation can realize third layer of sealedly, can carry out three-layer seal between bottle lid and the bottle base member, and the sealing ring and the butt joint groove ring of bottle lid lower extreme realize the sealing between bottle lid and the cavity ring, realize the sealing of culture medium in transportation or placing process.
As a further improvement of the invention, the auxiliary sealing unit comprises a sealing ring adhered to the lower end of the bottle cap, the upper end of the bottom wall of the cavity ring is provided with a butt joint groove ring, and the sealing ring is matched with the butt joint groove ring.
As a further improvement of the invention, the four corners of the base are respectively provided with mounting holes, or the lower end of the base is adhered with double faced adhesive tape.
As a further improvement of the present invention, a label is attached to the outer circumferential surface of the bottle-shaped base body.
Drawings
The invention will be described in further detail with reference to the drawings and the detailed description.
FIG. 1 is a schematic structural view of embodiment 1 of the present invention;
FIG. 2 is a cross-sectional view of FIG. 1;
FIG. 3 is a schematic perspective view of the inner bracket in FIG. 2;
FIG. 4 is a schematic structural view of embodiment 2 of the present invention;
FIG. 5 is a schematic diagram of the explosive structure of FIG. 4;
FIG. 6 is a schematic structural view of a fixing assembly in embodiment 2 of the present invention;
FIG. 7 is a cross-sectional view of FIG. 6;
FIG. 8 is a schematic view of the bottle-shaped base body in example 2 of the present invention;
FIG. 9 is an enlarged schematic view of FIG. 8 at A;
FIG. 10 is a cross-sectional view of a bottle-shaped base body in example 2 of the present invention;
FIG. 11 is an enlarged schematic view at B in FIG. 10;
fig. 12 is a schematic view showing the structure of the bottle cap and the inner holder in embodiment 2 of the present invention.
In the figure: 101. a base; 102. a mounting hole; 103. a bottle-shaped base; 104. scale marks; 105. a label; 106. a clamping cylinder; 107. an inner support; 108. a filter membrane cartridge; 109. a clamping block; 110. a clamping groove frame; 111. installing a spring; 112. placing a tray; 114. a baffle; 115. a flange; 116. a positioning cylinder; 117. a storage cylinder; 118. a detachable pull ring; 119. an extension cylinder; 201. a cavity ring; 202. pressing a disc; 203. a bottle cap; 204. the anti-slip boss I; 205. a rod body; 206. a jack; 207. a connecting rod; 208. inserting a column; 209. a return spring; 210. a locking frame; 211. a second anti-slip boss; 212. an adjusting seat; 213. a sliding column; 301. sticking a ring; 302. a rectangular seal groove; 303. an arc-shaped sealing groove; 304. a trapezoidal seal groove; 305. a rectangular sealing ring; 306. an arc-shaped sealing ring; 307. a trapezoid sealing ring; 308. a butt joint groove ring; 309. and (3) a sealing ring.
Detailed Description
For a better understanding of the present invention, the following examples are set forth to further illustrate the invention, but are not to be construed as limiting the invention. In the following description, numerous specific details are set forth in order to provide a more thorough understanding of the present invention. It will be apparent, however, to one skilled in the art that the invention may be practiced without one or more of these details.
Specific example 1 of bottle-type Medium apparatus of the invention:
as shown in fig. 1, 2 and 3, the bottle-shaped culture medium device comprises a bottle-shaped base 103, an inner support 107 and a bottle cap 203, and is integrally made of plastic material.
The bottle-shaped base 103 is used for storing liquid medium, and the inner support 107 is used for storing solid medium. As shown in fig. 2, the bottle cap 203 is in threaded connection with the bottle-shaped base 103, the bottle cap 203 is coaxially connected with the positioning cylinder 116 and the rod 205, and the rod 205 is positioned in the positioning cylinder 116 and the lower end of the rod 205 extends out of the bottle cap 203. The outer circumferential surface of the bottle cap 203 is provided with a plurality of first cleats 204.
As shown in FIG. 3, the inner support 107 includes a storage cylinder 117 and a ring of flanges 115 attached to the top of the storage cylinder 117, the flanges 115 having an outer diameter greater than the caliber of the bottle-shaped base 103. The reservoir cylinder 117 is adapted to be inserted into the bottle-shaped base 103 and is in a stop fit with the bottle-shaped base 103 via the flange 115. The inner bottom wall of the storage cylinder 117 is provided with a detachable baffle 114, and the baffle 114 is used for preventing the solid culture medium from entering the bottle-shaped base 103. The bottom of the storage cylinder 117 is also provided with an extension cylinder 119, the extension cylinder 119 is sleeved with a filtering membrane cylinder 108, and the filtering membrane cylinder 108 is internally provided with a filtering membrane positioned below the baffle 114. The filtering membrane can filter out impurities mixed in the solid culture medium, and can shield the baffle 114 at the same time, so that the baffle is prevented from falling into the bottle-shaped matrix 103.
The upper end of the bottle-shaped base 103 is detachably connected with a limiting component for limiting the bottle cap 203, in this embodiment, the limiting component comprises a detachable pull ring 118, and a ring buckle for pulling by a person is arranged on the detachable pull ring 118. A sealing assembly is further arranged between the bottle-shaped substrate 103 and the bottle cap 203, and the sealing assembly comprises a plurality of stages of sealing rings which are arranged at intervals from top to bottom, so that leakage of the liquid culture medium can be prevented.
The cylinder inner diameter of the storage cylinder 117 gradually decreases from top to bottom, and the cylinder outer diameter of the positioning cylinder 116 is smaller than the maximum cylinder inner diameter of the storage cylinder 117 and larger than the minimum cylinder inner diameter of the storage cylinder 117, so that the positioning cylinder 116 can be in interference fit with the storage cylinder 117 after being inserted into the storage cylinder 117.
When the removable tab 118 is removed, pressing the cap 203 downward causes the lever 205 to eject the flap 114 from the storage cylinder 117 to mix the solid media in the inner support 107 with the liquid media in the bottle-shaped base 103. In practical use, the liquid level of the liquid culture medium in the bottle-shaped matrix 103 can be added below the filtering membrane, and after the baffle 114 is poked, the bottle-shaped matrix 103 can be manually shaken to make the solid culture medium drop from the openings at the baffle 114 fully. The bottle-shaped substrate 103 may be shaken upside down to achieve sufficient contact of the solid medium with the liquid medium.
After the removable tab 118 is removed, pressing the cap 203 downward also causes the positioning cylinder 116 to be inserted into the storage cylinder 117 and to have an interference fit with the storage cylinder 117, thereby allowing the inner support 107 to be removed from the bottle-shaped base along with the cap 203.
Specific example 2 of bottle-type Medium apparatus of the invention:
as shown in fig. 4, 5 and 6, the bottle-shaped culture medium device comprises a base 101, a clamping barrel 106 is arranged at the upper end of the base 101, a placing disc 112 is slidably connected in the clamping barrel 106, a bottle-shaped substrate 103 is placed at the upper end of the placing disc 112, a plurality of scale marks 104 which are arranged at equal intervals along the upper and lower directions are arranged at the front side of the outer arc circumference of the bottle-shaped substrate 103, and a label 105 is adhered to the right side of the outer arc circumference of the bottle-shaped substrate 103. The inside of joint section of thick bamboo 106 is provided with the fixed subassembly of fixed bottle type base member 103, and the upper end of bottle type base member 103 is provided with the spacing subassembly that carries out spacing to bottle lid 203, and the upper end of bottle type base member 103 is provided with seal assembly. In this embodiment, as shown in fig. 6 and 7, the fixing assembly includes two clamping groove frames 110 disposed on the inner arc wall of the clamping barrel 106, the two clamping groove frames 110 are located in left and right positions, two clamping blocks 109 are disposed at the lower end of the outer arc circumferential surface of the bottle-shaped base 103, the clamping blocks 109 are respectively mounted in cooperation with the adjacent clamping groove frames 110, and a mounting spring 111 is disposed between the bottom wall of the clamping barrel 106 and the placing tray 112. Mounting holes 102 are respectively formed in four corners of the base 101, the fixing assembly is fixed through the mounting holes 102, double faced adhesive tape can be adhered to the lower end of the base 101, and the fixing assembly is adhered to a proper position through the double faced adhesive tape.
As shown in fig. 10, 11 and 12, the sealing assembly comprises a rectangular sealing ring 305 adhered to the upper end of the bottle-shaped substrate 103, a rectangular sealing groove 302 is formed in the upper end of the inner cambered surface of the bottle cap 203, the rectangular sealing ring 305 is installed in cooperation with the rectangular sealing groove 302, a trapezoid sealing ring 307 is adhered to the lower end of the bottle-shaped substrate 103, a trapezoid sealing groove 304 is formed in the lower end of the inner cambered surface of the bottle cap 203, an arc sealing ring 306 positioned between the rectangular sealing ring 305 and the trapezoid sealing ring 307 is installed in cooperation with the trapezoid sealing groove 304, an arc sealing groove 303 is formed in the inner cambered surface of the bottle cap 203, an adhesive ring 301 is installed in cooperation with the inner support 107, an auxiliary sealing unit is arranged at the lower end of the bottle cap 203, the auxiliary sealing unit comprises a sealing ring 309 adhered to the lower end of the bottle cap 203, a butt joint groove ring 308 is arranged at the upper end of the bottom wall of the cavity ring 201, and the sealing ring 309 is installed in cooperation with the butt joint groove ring 308.
As shown in fig. 8, 9 and 10, the limiting assembly comprises a cavity ring 201 disposed at the upper end of the bottle-shaped base body 103, two locking frames 210 are slidably connected to the cavity ring 201 through two inserting columns 208, the two inserting columns 208 are respectively located at the front side and the rear side of the bottle-shaped base body 103 in the radial direction, one end of each inserting column 208 penetrates out of the cavity ring 201, and the end is provided with a blocking head for blocking and matching with the cavity ring 201. The outer arc circumferential surface of the bottle cap 203 is provided with a jack 206 corresponding to each plug 208, the jack 206 is in plug-in fit with the plug 208, a reset spring 209 is connected between the cavity ring 201 and each locking frame 210, and the reset spring 209 is sleeved outside the corresponding plug 208. The two ends of each locking frame 210 are respectively connected with connecting rods 207 in a rotating way, and the two connecting rods 207 positioned on the same locking frame 210 are symmetrical relative to a return spring 209 connected on the locking frame 210.
Two adjusting seats 212 are respectively connected with the cavity ring 201 in a sliding manner through two sliding columns 213, the two adjusting seats 212 are respectively positioned at the radial left side and the radial right side of the bottle-shaped base body 103, one end of each sliding column 213 extends out of the cavity ring 201 and is connected with a pressing disc 202, and a plurality of second anti-slip protrusions 211 are arranged on the pressing surface of the pressing disc 202. One end of the two connecting rods 207 which are rotatably connected to the same locking frame 210 and far away from the locking frame 210 is rotatably connected to the corresponding ends of the two adjusting seats 212.
The personnel can prevent the hand from sliding with the pressing plate 202 through pressing the pressing plate 202 on both sides, the adjusting seat 212 on the left side and the right side is driven to be close to each other through the sliding column 213, the connecting rod 207 in the adjusting seat 212 rotates, the front locking frame 210 and the rear locking frame 210 are driven to be far away from each other under the limitation of the inserting column 208, the inserting column 208 is separated from the inserting column 206, the reset spring 209 is in a compressed state, the personnel upwards removes the bottle cap 203 from the bottle-shaped base body 103, the first anti-slip bump 204 on the bottle cap 203 can prevent the personnel from sliding, the personnel takes out the inner support 107 to be inwards placed into the solid culture medium, then the liquid culture medium is placed into the bottle-shaped base body 103, accurate filling can be achieved through observing the scale marks 104, the inner support 107 filled with the solid culture medium is placed into the bottle-shaped base body 103, the bottle cap 203 is placed onto the upper end of the bottle-shaped base body 103, the bottle cap 203 is pressed downwards, the inserting column 208 is aligned with the inserting column 208 is enabled, the reset spring 209 is released, the personnel can quickly drive the reset spring 209 to rebound the bottle cap 203 from the bottle-shaped base body 103 to be removed from the bottle-shaped base body 103, the inserting column 206 is inserted into the bottle-shaped base body, and the bottle cap body is assembled.
In the transportation or placement process, the rectangular sealing ring 305 contacts with the rectangular sealing groove 302 to prevent the overflow of the liquid culture medium, so that the first-layer sealing is realized, the arc sealing ring 306 is extruded into the arc sealing groove 303 to form the second-layer sealing, the trapezoid sealing ring 307 cooperates with the trapezoid sealing groove 304 to realize the third-layer sealing, the bottle cap 203 and the bottle-shaped substrate 103 can be sealed in three layers, and the sealing ring 309 at the lower end of the bottle cap 203 and the butt joint groove ring 308 realize the sealing between the bottle cap 203 and the cavity ring 201.
When the culture medium filter membrane device is used, a person firstly fixes the base 101 at a proper position, then presses the pressing plates 202 on two sides, then drives the adjusting seats 212 on the left side and the right side to be close to each other through the sliding columns 213, so that the connecting rods 207 in the adjusting seats 212 rotate, further drives the front and rear locking frames 210 to be far away from each other under the limitation of the inserting columns 208, the inserting columns 208 leave the inside of the inserting holes 206, then continues to press the bottle cap 203, so that the sealing rings 309 are extruded with the butt-joint groove rings 308, the rod bodies 205 are led to puncture the inner support 107, at the moment, the adhesive rings 301 are contacted with the inner support 107, solid culture medium in the inner support 107 is filtered by the filter membrane cylinders 108 and mixed with liquid culture medium, the filter membrane cylinders 108 can enable solid particles with the aperture of more than 400um to not pass through, further, when the solid particles are prevented from falling into the bottle-shaped base 103 during mixing, then the person takes out the bottle cap 203, the adhesive rings 301 in the bottle cap 203 are adhered to the inner support 107 and the filter membrane cylinders 108, takes out together with the bottle cap 203, and the mixed culture medium is convenient for the person to use, and the mixed culture medium is prevented from being polluted.
After the bottle-shaped matrix 103 is aligned with the placing disc 112 and pressed downwards by a person after the bottle-shaped matrix 103 is used, the placing disc 112 moves downwards, the mounting spring 111 is compressed at the moment, then the person rotates the bottle-shaped matrix 103 to enable the clamping block 109 to rotate to the inside of the clamping groove frame 110, then the person releases hands, at the moment, the mounting spring 111 rapidly rebounds to drive the bottle-shaped matrix 103 on the placing disc 112 to move upwards, the clamping block 109 is propped against the clamping groove frame 110, and therefore the bottle-shaped matrix 103 is fixed, the bottle-shaped matrix 103 can be prevented from being impacted to the influence of the person, and the person can conveniently take and place the bottle-shaped matrix 103 at any time.
Finally, it is noted that the above-mentioned embodiments are merely for illustrating the technical solution of the present invention, and that other modifications and equivalents thereof by those skilled in the art should be included in the scope of the claims of the present invention without departing from the spirit and scope of the technical solution of the present invention.
Claims (4)
1. A bottle-shaped culture medium device is characterized by comprising a bottle-shaped base body (103), an inner support (107) and a bottle cap (203), wherein the bottle-shaped base body (103) is used for storing a liquid culture medium, and the inner support (107) is used for storing a solid culture medium;
the bottle cap (203) is in threaded connection with the bottle-shaped base body (103), a positioning cylinder (116) and a rod body (205) are coaxially connected in the bottle cap (203), the rod body (205) is positioned in the positioning cylinder (116), and the lower end of the rod body extends out of the bottle cap (203);
the inner support (107) comprises a storage cylinder body (117) and a circle of flange (115) connected to the top of the storage cylinder body (117) and used for being in stop fit with the bottle-shaped base body (103), the storage cylinder body (117) is used for being inserted into the bottle-shaped base body (103), a detachable baffle (114) is arranged on the inner bottom wall of the storage cylinder body (117), the baffle (114) is used for preventing a solid culture medium from entering the bottle-shaped base body (103), the bottom of the storage cylinder body (117) is also sleeved with a filtering membrane cylinder (108), and a filtering membrane positioned below the baffle (114) is arranged in the filtering membrane cylinder (108);
the upper end of the bottle-shaped base body (103) is detachably connected with a limiting assembly for limiting the bottle cap (203), and a sealing assembly is arranged between the bottle-shaped base body (103) and the bottle cap (203);
after the limiting component is removed, the bottle cap (203) is pressed downwards, so that the rod body (205) can eject the baffle (114) from the storage cylinder (117) to enable the solid culture medium in the inner support (107) to be mixed with the liquid culture medium in the bottle-shaped matrix (103); after the limiting component is removed, the bottle cap (203) is pressed downwards, so that the positioning cylinder (116) can be inserted into the storage cylinder (117) and connected with the storage cylinder (117);
the inner diameter of the storage cylinder (117) gradually decreases from top to bottom, and the outer diameter of the positioning cylinder (116) is smaller than the maximum inner diameter of the storage cylinder (117) and larger than the minimum inner diameter of the storage cylinder (117), so that the positioning cylinder (116) can be in interference fit with the storage cylinder (117) after being inserted into the storage cylinder (117);
the bottle-shaped base body is characterized in that the bottom of the bottle-shaped base body is provided with a base (101), the upper end of the base (101) is provided with a clamping barrel (106), the inside of the clamping barrel (106) is connected with a placing disc (112) in a sliding manner along the up-down direction, the placing disc (112) is used for placing the bottle-shaped base body (103), and a fixing component used for fixing the bottle-shaped base body (103) is arranged in the clamping barrel (106); scale marks (104) which are arranged at equal intervals along the up-down direction are arranged on the outer arc circumferential surface of the bottle-shaped matrix (103);
the fixing assembly comprises two clamping groove frames (110) arranged on the inner arc wall of the clamping cylinder (106), the left and right positions of the two clamping groove frames (110) correspond to each other, two clamping blocks (109) are arranged at the lower end of the outer arc circumferential surface of the bottle-shaped base body (103), the clamping blocks (109) are used for being clamped into the corresponding clamping groove frames (110), mounting springs (111) with two ends respectively connected with the placing disc (112) and the base (101) are arranged in the clamping cylinder (106), and the mounting springs (111) are used for pushing the bottle-shaped base body (103) so that the clamping blocks (109) are in blocking fit with the clamping groove frames (110);
the limiting assembly comprises a detachable pull ring (118) which surrounds the periphery of the bottle-shaped base body (103), and the detachable pull ring (118) is in stop fit with the bottle cap (203) in the up-down direction;
or the limiting component comprises a cavity ring (201) arranged at the upper end of the bottle-shaped matrix (103), two locking frames (210) are respectively connected with the cavity ring (201) in a sliding way through two inserting columns (208), the two inserting columns (208) are respectively positioned at the front side and the rear side of the bottle-shaped matrix (103) in the radial direction, and one end of each inserting column (208) penetrates out of the cavity ring (201) and is connected with a blocking head which is used for being matched with the cavity ring (201) in a blocking way; a jack (206) for being in plug-in fit with each plug-in column (208) is arranged on the outer arc circumferential surface of the bottle cap (203) at a position corresponding to each plug-in column (208), a reset spring (209) is connected between the cavity ring (201) and each locking frame (210), and the reset spring (209) is sleeved outside the corresponding plug-in column (208); the two ends of each locking frame (210) are respectively and rotatably connected with a connecting rod (207), and the two connecting rods (207) positioned on the same locking frame (210) are symmetrical relative to a return spring (209) connected with the locking frame (210); two adjusting seats (212) are respectively connected with the cavity ring (201) in a sliding manner through two sliding columns (213), the two adjusting seats (212) are respectively positioned at the left side and the right side of the radial direction of the bottle-shaped base body (103), and one end of each sliding column (213) extends out of the cavity ring (201) and is connected with a pressing disc (202); one end, far away from the locking frame (210), of two connecting rods (207) which are rotationally connected on the same locking frame (210) is rotationally connected with corresponding ends of two adjusting seats (212) respectively;
the sealing assembly comprises a rectangular sealing ring (305), an arc-shaped sealing ring (306) and a trapezoid sealing ring (307) which are adhered to the upper end of the bottle-shaped substrate (103), wherein a rectangular sealing groove (302) is formed in the upper end of the inner cambered surface of the bottle cap (203), and the rectangular sealing ring (305) and the rectangular sealing groove (302) are installed in a matched mode; a trapezoid sealing groove (304) is formed in the lower end of the intrados of the bottle cap (203), and the trapezoid sealing ring (307) is installed in a matched mode with the trapezoid sealing groove (304); an arc-shaped sealing groove (303) is formed between the rectangular sealing groove (302) and the trapezoid sealing groove (304) on the inner cambered surface of the bottle cap (203), the arc-shaped sealing ring (306) is matched with the arc-shaped sealing groove (303), a sticky ring (301) is arranged on the top wall of the bottle cap (203), the sticky ring (301) is matched with the inner support (107), and an auxiliary sealing unit is arranged at the lower end of the bottle cap (203);
the auxiliary sealing unit comprises a sealing ring (309) adhered to the lower end of the bottle cap (203), a butt joint groove ring (308) is arranged at the upper end of the bottom wall of the cavity ring (201), and the sealing ring (309) is installed in a matched mode with the butt joint groove ring (308).
2. The bottle-type medium device according to claim 1, wherein: a plurality of first anti-slip protrusions (204) are arranged on the outer arc circumferential surface of the bottle cap (203), and a plurality of second anti-slip protrusions (211) are arranged on the pressing surface of the pressing disc (202).
3. The bottle-type medium device according to claim 1, wherein: mounting holes (102) are respectively formed in four corners of the base (101), or double faced adhesive tape is adhered to the lower end of the base (101).
4. The bottle-type medium device according to claim 1, wherein: a label (105) is attached to the outer peripheral surface of the bottle-shaped base body (103).
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| CN202311743270.7A CN117417815B (en) | 2023-12-19 | 2023-12-19 | Bottle-shaped culture medium device |
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| CN202311743270.7A CN117417815B (en) | 2023-12-19 | 2023-12-19 | Bottle-shaped culture medium device |
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| CN117417815B true CN117417815B (en) | 2024-03-12 |
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