CN117388482A - Detection analysis device and corresponding detection analysis system - Google Patents
Detection analysis device and corresponding detection analysis system Download PDFInfo
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- CN117388482A CN117388482A CN202210779792.1A CN202210779792A CN117388482A CN 117388482 A CN117388482 A CN 117388482A CN 202210779792 A CN202210779792 A CN 202210779792A CN 117388482 A CN117388482 A CN 117388482A
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- accommodating cavity
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- 238000001514 detection method Methods 0.000 title claims abstract description 196
- 238000004458 analytical method Methods 0.000 title claims abstract description 64
- 239000012528 membrane Substances 0.000 claims abstract description 118
- 238000012360 testing method Methods 0.000 claims abstract description 104
- 238000002347 injection Methods 0.000 claims abstract description 60
- 239000007924 injection Substances 0.000 claims abstract description 60
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 57
- 239000012780 transparent material Substances 0.000 claims description 5
- 238000010438 heat treatment Methods 0.000 claims description 4
- 230000007246 mechanism Effects 0.000 claims description 4
- 238000003556 assay Methods 0.000 claims 4
- 239000007788 liquid Substances 0.000 abstract description 93
- 239000007790 solid phase Substances 0.000 abstract description 4
- 230000009257 reactivity Effects 0.000 abstract description 3
- 238000005406 washing Methods 0.000 description 45
- 238000007789 sealing Methods 0.000 description 22
- 239000011358 absorbing material Substances 0.000 description 13
- 239000007853 buffer solution Substances 0.000 description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 13
- 238000002360 preparation method Methods 0.000 description 12
- 210000002966 serum Anatomy 0.000 description 11
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 8
- 238000000034 method Methods 0.000 description 6
- 230000035945 sensitivity Effects 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- 238000001917 fluorescence detection Methods 0.000 description 4
- 241001678559 COVID-19 virus Species 0.000 description 3
- 239000000427 antigen Substances 0.000 description 2
- 102000036639 antigens Human genes 0.000 description 2
- 108091007433 antigens Proteins 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 241000711573 Coronaviridae Species 0.000 description 1
- 108010047320 Pepsinogen A Proteins 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000002795 fluorescence method Methods 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
Abstract
The invention relates to a detection and analysis device and a corresponding detection and analysis system, wherein the detection and analysis device comprises a detection box, a film frame for fixing a test film and a pushing device; the inner part of the detection box is provided with a containing cavity, and the outer surface of the detection box is provided with two pushing injection ports communicated with the containing cavity; the film frame is arranged in the accommodating cavity and between the two pushing injection ports, and an included angle between a plane formed by the film frame and the bottom surface of the accommodating cavity is between 45 degrees and 90 degrees; the injector is detachably fixed at the injection port and is communicated with the accommodating cavity in the detection box; the test membrane in the system is arranged in the accommodating cavity through the membrane frame. The detection analysis device adopting the structure and the corresponding detection analysis system can repeatedly push and suck out the liquid in the detection box through the injector, so that the liquid repeatedly flows through the test membrane, the reagent is effectively promoted to pass through the porous solid phase membrane, the reactivity of the detection system is improved by increasing the contact frequency between active elements, and the detection efficiency of the reagent is effectively improved.
Description
Technical Field
The invention relates to the technical field of medical instruments, in particular to a detection and analysis device and a corresponding detection and analysis system.
Background
In the field of detection analysis, sensitivity in detection is a common problem encountered in test development and improvement. Only effectively improving the sensitivity in detection can detect more rapidly and effectively. Although the sensitivity of detection can be improved by heating or using special instruments in laboratory environment, the factors that can be optimized in the solid phase immunoassay without instrument and equipment assistance, which is accomplished at normal temperature other than laboratory, are very limited. One tries to adjust the reagent dosage, increase the sample size, and optimize the reagent formulation and raw materials used, but all of these methods have their limitations as they may not be suitable for the relevant test formats, procedures and techniques employed.
Disclosure of Invention
The invention aims to overcome the defects in the prior art, and provides a detection analysis device and a corresponding detection analysis system which are convenient to use and can effectively improve the detection sensitivity under the normal-temperature environment.
In order to achieve the above object, a detection and analysis device and a detection and analysis system according to the present invention are as follows:
the detection and analysis device is mainly characterized by comprising a detection box, a membrane frame and a pushing device;
the detection box is internally provided with a containing cavity, and the outer surface of the detection box is provided with two pushing injection ports communicated with the containing cavity;
the membrane frame is used for fixing an external test membrane, is arranged in the accommodating cavity and is positioned between the two pushing injection ports, and an included angle between a plane formed by the membrane frame and the bottom surface of the accommodating cavity is 45-90 degrees;
the injector is detachably fixed at the injection port and is communicated with the accommodating cavity in the detection box through the injection port.
The detection and analysis device is characterized in that a film frame fixing groove is formed in the accommodating cavity, and the film frame is detachably inserted into the film frame fixing groove to be fixed in the accommodating cavity.
The detection and analysis device comprises two push syringes, wherein the two push syringes are detachably connected with the two push ports respectively.
The detection and analysis device, wherein each injector is in threaded connection with the corresponding injection port.
In the detection and analysis device, the detection cartridge is formed by sealing and closing an upper cover and a lower cover, and the accommodating cavity is formed by a space between the upper cover and the lower cover.
The detection and analysis device is characterized in that the detection box is made of transparent materials.
The detection and analysis system is characterized by comprising a test membrane and the detection and analysis device, wherein the test membrane is arranged in the accommodating cavity through the membrane frame.
The detection and analysis system further comprises a reagent injection assembly, wherein the reagent injection assembly is used for injecting the reagent into the accommodating cavity through any pushing port.
The detection and analysis system, wherein the reagent injection assembly is composed of a pipette or a drip bottle.
The detection and analysis system further comprises an automatic bolus assembly, wherein the automatic bolus assembly is connected with the bolus device.
The detection and analysis system is characterized in that a heating mechanism is further arranged at a position, in contact with the injector, of the automatic injection assembly.
The detection and analysis device and the corresponding detection and analysis system have the following beneficial effects:
in the embodiment, the membrane frame is arranged in the detection box, so that the test membrane is arranged in the accommodating cavity through the membrane frame and is positioned between the two pushing ports of the detection box, and when the detection box is used, liquid in the accommodating cavity of the detection box can be repeatedly pushed and sucked from the pushing ports through the pushing device, so that the liquid repeatedly flows through the test membrane, the reagent is effectively promoted to pass through the porous solid-phase membrane, the reactivity of a detection system is improved by increasing the contact frequency between active elements, and the detection efficiency of the reagent is effectively improved. The detection and analysis device and the system comprising the detection and analysis device are simple in structure, convenient to operate, capable of effectively improving detection efficiency and wide in application range.
Drawings
The conception, specific structure, and technical effects of the present invention will be further described with reference to the accompanying drawings to fully understand the objects, features, and effects of the present invention.
FIG. 1 is a schematic diagram of a detection analysis system in an embodiment.
Drawings
1. Detection box
11. Upper cover
12. Lower cover
13. First push injection port
14. Second injection port
15. Film frame fixing groove
2. Membrane frame
3. First injector
4. Second injector
5. Test membrane
Detailed Description
The invention is further described with reference to the following detailed description in order to make the technical means, the inventive features, the achieved objects and the effects of the invention easy to understand. The present invention is not limited to the following examples.
It should be understood that the structures, proportions, sizes, etc. shown in the drawings are for illustration purposes only and should not be construed as limiting the invention to the extent that it can be practiced, since modifications, changes in the proportions, or otherwise, used in the practice of the invention, are not intended to be critical to the essential characteristics of the invention, but are intended to fall within the spirit and scope of the invention.
Example 1
As shown in fig. 1, the detection and analysis system in this embodiment includes a test membrane 5 and a detection and analysis device, wherein the detection and analysis device includes a detection cartridge 1, a membrane frame 2, and a syringe;
the inside of the detection box 1 is provided with a containing cavity for containing the test membrane 5, the outer surface of the detection box 1 is provided with two injection ports, as shown in fig. 1, the two injection ports are a first injection port 13 and a second injection port 14 respectively, and the first injection port 13 and the second injection port 14 are communicated with the containing cavity;
the membrane frame 2 is located between the first pushing port 13 and the second pushing port 14, the membrane frame 2 comprises four membrane fixing strips, wherein the four membrane fixing strips are two membrane side fixing strips, a membrane fixing top strip and a membrane fixing bottom strip respectively, the two membrane side fixing strips are symmetrically arranged on two side walls of the accommodating cavity respectively, the membrane fixing top strip is located on the top surface of the accommodating cavity, two ends of the membrane fixing top strip are connected with the two membrane side fixing strips respectively, the membrane fixing bottom strip is located on the bottom surface of the accommodating cavity, two ends of the membrane fixing bottom strip are connected with the two membrane side fixing strips respectively, and an included angle between each membrane side fixing strip and the bottom surface of the accommodating cavity is between 45 DEG and 90 DEG, so that an included angle between a plane formed by the membrane frame 2 and the bottom surface of the accommodating cavity is between 45 DEG and 90 DEG, and further an included angle between the test membrane 5 fixed in the accommodating cavity through the membrane frame 2 and the bottom surface of the accommodating cavity is also between 45 DEG and 90 deg. In other embodiments, the test membrane 5 may be fixed in the accommodating cavity by a membrane frame including only two or three membrane fixing strips, where the position of the test membrane 5 where no membrane fixing strip is disposed may seal a gap between the test membrane 5 and the accommodating cavity by providing an additional sealing member, so as to ensure that the liquid flowing in the accommodating cavity passes through the test membrane 5 each time, but not through the gap between the test membrane 5 and the inner wall of the accommodating cavity. In still other embodiments, the membrane frame may be designed to have other shapes that match the receiving cavity, and is not necessarily rectangular. In the embodiment, two ejectors, namely a first ejector 3 and a second ejector 4 are arranged, the first ejector 3 and the second ejector 4 are respectively in threaded connection with a first ejector port 13 and a second ejector port 14, and the ejectors are used for executing the ejecting operation to repeatedly push and suck liquid into the detection box 1 so as to drive the liquid to flow in the detection box 1;
because the included angle between the test membrane 5 and the bottom surface of the accommodating cavity is between 45 degrees and 90 degrees, when the liquid flows in the detecting box 1, the surface of the test membrane 5 forms an angle of 45 degrees to 90 degrees with the liquid flow, and the test membrane 5 is positioned between the first pushing port 13 and the second pushing port 14 (namely, the test membrane 5 is positioned in the middle of the detecting box 1), so that the test membrane 5 can be ensured to become a necessary path for the liquid in the accommodating cavity to flow.
In this embodiment, two ejectors are provided, in the operation process, after the liquid is injected into the detection box 1 by the ejector on one side, the liquid originally located in the detection box 1 can be extruded into the ejector on the other side, so that the liquid is always fully injected into the detection box 1 in the experiment process, the influence of redundant gas entering the detection box 1 on the experiment is avoided, and the efficiency of the experiment is ensured. In other examples, although a single injector may be used to inject liquid into the test cassette 1 from one side of the injector and then to withdraw liquid from the test cassette 1 from the other side of the injector, this embodiment is less efficient and cumbersome to operate than the embodiment in which each injector is provided with an injector, and because the other injector on the test cassette must be kept open to release the pressure in the cassette during injection, the test cassette is less airtight, and liquid spraying is likely to occur during injection.
Meanwhile, because each injector is in threaded connection with the corresponding injection port in the embodiment, the injector can be fixed on the corresponding injection port when being matched with the detection box 1, and the injector can play a role in sealing the detection box 1 when being fixed on the injection port, so that liquid leakage is avoided when the injector repeatedly pushes and sucks liquid in the detection box 1.
In this embodiment, the detection cartridge 1 is made of a transparent material, and by constituting the detection cartridge 1 by selecting a transparent material, an operator can more conveniently observe the liquid state inside the detection cartridge 1 and can more conveniently read the detection result.
In practical implementation, a specific included angle between the test membrane 5 and the bottom surface of the accommodating cavity can be designed according to practical requirements, and when the included angle between the test membrane 5 and the bottom surface of the accommodating cavity is smaller, the surface area of the test membrane 5 is larger, so that more detection items can be implemented on one test membrane (namely, the test membrane obliquely arranged in the accommodating cavity has larger surface area compared with the test membrane vertically arranged in the accommodating cavity). And because the detection box 1 is made of transparent materials, compared with the test membrane vertically arranged in the accommodating cavity, the observability of the test membrane obliquely arranged in the accommodating cavity is better, and a user can observe the reaction result on the obliquely arranged test membrane from more view angles.
In this embodiment, the housing cavity is provided with a film frame fixing groove 15, and the film frame 2 is detachably inserted into the film frame fixing groove 15, so that the film frame 2 for fixing the test membrane 5 is detachably disposed in the housing cavity, and the film frame 2 with the test membrane 5 can be more conveniently installed in the detection box 1.
In this embodiment, the detection cartridge 1 is formed by sealing and closing the upper cover 11 and the lower cover 12, a housing cavity is formed by a space between the upper cover 11 and the lower cover 12, a film frame fixing groove 15 is provided in the housing cavity, and the film frame 2 is detachably inserted into the film frame fixing groove 15, so that the film frame 2 for fixing the test membrane 5 is detachably provided in the housing cavity, and after the test membrane 5 is placed, the upper cover 11 and the lower cover 12 are closed, by this structure, the film frame 2 with the test membrane 5 can be conveniently mounted and taken out, and leakage is effectively avoided.
In this embodiment, as shown in fig. 1, the injection port is disposed in the outer convex ring on the upper surface of the detection cartridge 1, the inner surface of the outer convex ring is provided with internal threads, the outlet of the injector is provided with external threads, and the injector is in threaded connection with the injection port through the external threads at the outlet and the internal threads on the inner surface of the outer convex ring.
In this embodiment, an additional reagent injection assembly is provided to inject reagent into the test cassette 1, which can inject reagent into the receiving cavity through the first push port 13 or the second push port 14. Wherein, the reagent injection assembly is constituted by a pipette or a drip bottle, and when the reagent injection assembly is constituted by a drip bottle, the reagent can be prestored in the drip bottle. In other embodiments, reagents may also be injected into the test cassette 1 directly through a syringe.
The invention does not need to arrange special equipment, only adopts the detection and analysis device and the corresponding detection and analysis system in the embodiment, does not need to greatly change the tested formula and other parts, does not need to increase the use cost of the reagent, can effectively improve the functional efficiency and the test sensitivity of the reagent with lower cost, improves the analysis performance and the test sensitivity, can be suitable for various normal-temperature immunity tests based on porous membranes, and has wide application range.
The general operation of the detection and analysis system is as follows:
1: the test cartridge 1 containing the test membrane 5 is prepared by the following specific operations: the test membrane 5 coated with the relevant active components for detection is arranged in the membrane frame 2, the membrane frame 2 embedded with the test membrane 5 is embedded in the membrane frame fixing groove 15 of the detection box 1, and the upper cover 11 and the lower cover 12 of the detection box 1 are closed;
2: the liquid feeding specifically operates as: adding a preset reagent into the detection box 1 through a first pushing port 13 or a second pushing port 14 by using a liquid gun or a prepared dripping bottle containing the preset reagent, etc., until the preset reagent is filled in a containing cavity of the detection box 1, and the containing cavity is free from too much air;
3: the injector is used for injecting, and specific operations comprise:
performing a pre-use preparation operation: one of the first injector 3 and the second injector 4 is in a push shape, and the other is in an extension shape and is full of relevant preset reagent, wherein the preset reagent amount in the injector is consistent with the preset reagent amount to be pushed in the detection cartridge 1; in performing this operation, the first injector 3 and the second injector 4 need to be screwed up at the first injector port 13 and the second injector port 14, respectively, wherein no leakage is required at the screw thread seal;
performing a bolus operation: the first and second syringes 3 and 4 are alternately injected such that a predetermined reagent flows back and forth in the test cartridge 1, repeatedly passing through the face of the intermediate test membrane 5. Wherein the duration or number of bolus injections depends on the test requirements;
performing a liquid removal operation: after the reaction is completed, the first and second syringes 3 and 4 are removed, the first and second syringe ports 13 and 14 are opened, and a predetermined reagent is removed with a pipette or a water absorbing material prepared in advance.
To enable those skilled in the art to better understand the operation of the detection and analysis system, two specific examples of operation are further described below:
example 1: the detection analysis system of the invention is used for carrying out enhanced detection (colloidal gold method) on the novel coronavirus (SARS-CoV-2) antibody
1. Preparing a detection box 1 containing a test membrane 5 coated with a known SARS-CoV-2 antigen, namely fixing the test membrane 5 coated with the known SARS-CoV-2 antigen on a membrane frame, then placing the membrane frame on a membrane frame fixing groove 15 of the detection box 1, and closing an upper cover 11 and a lower cover 12 of the detection box 1;
2. sealing test membrane 5
2.1, adding a preset reagent, namely a sealing liquid, into a containing cavity of the detection box 1 through a first pushing port 13 and a second pushing port 14 on the detection box 1, and filling all spaces of the containing cavity with the sealing liquid;
2.2 bolus operations: the injector is used for injecting, and specific operations comprise:
performing a pre-use preparation operation: one of the first injector 3 and the second injector 4 is in a pushing shape, and the other is in an extending shape and is full of sealing liquid, wherein the quantity of the sealing liquid in the injector is consistent with the quantity of the sealing liquid to be pushed in the detection box 1;
performing a bolus operation: tightening the first and second ejectors 3, 4 on the first and second ejector ports 13, 14, respectively, alternately pushing the first and second ejectors 3, 4 so that the sealing liquid flows back and forth in the detection cartridge 1, repeatedly passing through the face of the middle test membrane 5, wherein the duration or number of ejectors depends on the test requirements;
and (3) performing a sealing liquid removing operation: after the reaction is finished, the first injector 3 and the second injector 4 are respectively unscrewed from the first injector 13 and the second injector 14, and a suction pipe or a water absorbing material prepared in advance is used for removing the sealing liquid in the accommodating cavity through the first injector 13 and/or the second injector 14;
3. and (3) performing membrane washing operation:
3.1, injecting a preset reagent, namely washing liquid, into the accommodating cavity of the detection box 1 through a first pushing and injecting port 13 or a second pushing and injecting port 14 on the detection box 1, and filling all the spaces of the accommodating cavity with the washing liquid;
3.2, performing a bolus operation: the injector is used for injecting, and specific operations comprise:
a syringe having a stretched shape and filled with a washing liquid, wherein the amount of the washing liquid in the syringe is identical to the amount of the washing liquid to be pushed in the cartridge 1;
performing a bolus operation: tightening the first and second ejectors 3, 4 on the first and second ejector ports 13, 14, respectively, alternately advancing the first and second ejectors 3, 4 such that the wash solution flows back and forth within the test cartridge 1, repeatedly across the face of the intermediate test membrane 5, wherein the duration or number of ejectors depends on the test requirements;
performing a liquid removal operation: after the reaction is finished, the first injector 3 and the second injector 4 are respectively unscrewed from the first injector 13 and the second injector 14, and the washing liquid in the accommodating cavity is removed through the first injector 13 and/or the second injector 14 by a suction pipe or a water absorbing material prepared in advance;
4. adding a sample:
4.1, adding a preset reagent, namely a buffer solution containing serum samples, into the accommodating cavity of the detection box 1 through a first pushing port 13 or a second pushing port 14 on the detection box 1, and filling all spaces in the accommodating cavity with the buffer solution containing serum samples;
4.2 bolus injection the bolus injector is used for bolus injection, and the specific operations comprise:
performing a pre-use preparation operation: one of the first injector 3 and the second injector 4 is in a pushing shape, the other is in an extending shape and is full of buffer solution, wherein the amount of the buffer solution in the injector is consistent with the amount of the buffer solution containing the serum sample to be pushed in the detection cartridge 1;
performing a bolus operation: tightening the first and second ejectors 3, 4 on the first and second ejector ports 13, 14, respectively, alternately pushing the first and second ejectors 3, 4 so that the buffer solution containing the serum sample flows back and forth in the test cartridge 1, repeatedly passing through the face of the middle test membrane 5, wherein the duration or number of ejectors depends on the test requirements;
performing a liquid removal operation: after the reaction is finished, the first injector 3 and the second injector 4 are respectively unscrewed from the first injector 13 and the second injector 14, and buffer liquid containing serum samples in the detection box 1 is removed through the first injector 13 and/or the second injector 14 by a suction pipe or a water absorbing material prepared in advance;
5. washing the film:
5.1, adding a preset reagent, namely washing liquid, into the accommodating cavity of the detection box 1 through a first pushing and injecting port 13 or a second pushing and injecting port 14 on the detection box 1, and filling all the spaces of the accommodating cavity with the washing liquid;
5.2 bolus injection the bolus injector is used for bolus injection, and the specific operations comprise:
performing a pre-use preparation operation: one of the first injector 3 and the second injector 4 is in a push shape, the other is in an extension shape and is full of the washing liquid, wherein the washing liquid in the injector is consistent with the amount of the washing liquid to be pushed in the detection box 1;
performing a bolus operation: the first injector 3 and the second injector 4 are screwed on the first injector port 13 and the second injector port 14 respectively, and the first injector 3 and the second injector 4 are alternately pushed so that the washing liquid flows back and forth in the detection cartridge 1 and repeatedly passes through the surface of the middle test membrane 5. Wherein the duration or number of bolus injections depends on the test requirements;
performing a liquid removal operation: after the reaction is finished, the first injector 3 and the second injector 4 are respectively unscrewed from the first injector 13 and the second injector 14, and the washing liquid in the detection box 1 is removed through the first injector 13 and/or the second injector 14 by a suction pipe or a water absorbing material prepared in advance;
6. and (3) detection:
6.1, adding a preset reagent, namely a colloidal gold detection reagent (a colloidal gold-marked anti-human secondary antibody), into the accommodating cavity of the detection box 1 through a first pushing port 13 or a second pushing port 14 on the surface of the detection box 1, and filling all the spaces of the accommodating cavity with the colloidal gold detection reagent;
6.2, performing a bolus operation: the injector is used for injecting, and specific operations comprise:
performing a pre-use preparation operation: one of the first injector 3 and the second injector 4 is in a pushing shape, and the other is in an extending shape and is full of colloidal gold detection reagent, wherein the colloidal gold detection test dose in the injector is consistent with the amount of the colloidal gold detection reagent to be pushed in the detection box 1;
performing a bolus operation: tightening the first injector 3 and the second injector 4 on the first injector port 13 and the second injector port 14 respectively, alternately pushing the first injector 3 and/or the second injector 4 so that the colloidal gold detection reagent flows back and forth in the accommodating cavity of the detection cartridge 1 and repeatedly passes through the surface of the middle test membrane 5, wherein the duration or the number of times of pushing depends on the test requirement;
performing a liquid removal operation: after the reaction is finished, the first injector 3 and the second injector 4 are respectively unscrewed from the first injector 13 and the second injector 14, and a suction pipe or a water absorbing material prepared in advance is used for removing the colloidal gold detection reagent in the detection box 1 through the first injector 13 and/or the second injector 14;
7. washing the film:
7.1, adding a preset reagent, namely washing liquid, into the accommodating cavity of the detection box 1 through a first pushing and injecting port 13 or a second pushing and injecting port 14 on the detection box 1, and filling all the space of the accommodating cavity with the washing liquid;
7.2 bolus injection the bolus injector is used for bolus injection, and the specific operations comprise:
performing a pre-use preparation operation: one of the first injector 3 and the second injector 4 is in a push shape, the other is in an extension shape and is full of the washing liquid, wherein the amount of the washing liquid in the injector is consistent with the amount of the washing liquid to be pushed in the detection cartridge 1;
performing a bolus operation: tightening the first and second ejectors 3, 4 on the first and second ejector ports 13, 14, respectively, alternately advancing the first and second ejectors 3, 4 such that the wash solution flows back and forth within the test cartridge 1, repeatedly across the face of the intermediate test membrane 5, wherein the duration or number of ejectors depends on the test requirements;
performing a liquid removal operation: after the reaction is finished, the first injector 3 and the second injector 4 are respectively unscrewed from the first injector 13 and the second injector 14, and the washing liquid in the detection box 1 is removed through the first injector 13 and/or the second injector 14 by a suction pipe or a water absorbing material prepared in advance;
8. reading the result: and obtaining a test result according to a related program.
Example 2: the pepsinogen is subjected to enhanced detection (fluorescence method) by adopting the detection and analysis system
1. Preparing a detection box 1 containing a test membrane 5 coated with anti-pepsinogen antibody, namely fixing the test membrane 5 coated with the anti-pepsinogen antibody on a membrane frame 2, then placing the membrane frame on a membrane frame fixing groove 15 of the detection box 1, and closing an upper cover 11 and a lower cover 12 of the detection box 1;
2. sealing test membrane 5:
2.1, adding a preset reagent, namely a sealing liquid, into the accommodating cavity of the detection box 1 through a first pushing port 13 or a second pushing port 14 on the detection box 1, and filling all the spaces of the accommodating cavity with the sealing liquid;
2.2 bolus operations: the injector is used for injecting, and specific operations comprise:
performing a pre-use preparation operation: one of the first injector 3 and the second injector 4 is in a pushing shape, and the other is in an extending shape and is full of sealing liquid, wherein the quantity of the sealing liquid in the injector is consistent with the quantity of the sealing liquid to be pushed in the detection box 1;
performing a bolus operation: the first injector 3 and the second injector 4 are screwed on the first injector port 13 and the second injector port 14 respectively, and the first injector 3 and the second injector 4 are alternately pushed so that the sealing liquid flows back and forth in the detection box 1 and repeatedly passes through the surface of the middle test membrane 5. Wherein the duration or number of bolus injections depends on the test requirements;
performing a liquid removal operation: after the reaction is finished, removing the first injector 3 and the second injector 4, opening the first injector 13 and the second injector 14, and removing the sealing liquid by using a suction tube or a water absorbing material prepared in advance;
3. and (3) performing membrane washing operation:
3.1, adding a preset reagent, namely washing liquid, into the accommodating cavity of the detection box 1 through a first pushing and injecting port 13 or a second pushing and injecting port 14 on the detection box 1, and filling all the spaces of the accommodating cavity with the washing liquid;
3.2, performing a bolus operation: the injector is used for injecting, and specific operations comprise:
performing a pre-use preparation operation: one of the first injector 3 and the second injector 4 is in a push shape, the other is in an extension shape and is full of the washing liquid, wherein the amount of the washing liquid in the injector is consistent with the amount of the washing liquid to be pushed in the detection cartridge 1;
performing a bolus operation: tightening the first and second ejectors 3, 4 on the first and second ejector ports 13, 14, respectively, alternately advancing the first and second ejectors 3, 4 such that the wash solution flows back and forth within the test cartridge 1, repeatedly across the face of the intermediate test membrane 5, wherein the duration or number of ejectors depends on the test requirements;
performing a liquid removal operation: after the reaction is finished, the first injector 3 and the second injector 4 are removed, the first injector 13 and the second injector 14 are opened, and washing liquid is removed by a suction tube or a water absorbing material prepared in advance;
4. adding a sample:
4.1, adding a preset reagent, namely a buffer solution containing a serum sample, into a containing cavity of the detection box 1 through a first pushing port 13 or a second pushing port 14 on the detection box 1, and filling all spaces of the containing cavity with the buffer solution containing the serum sample;
4.2 bolus injection the bolus injector is used for bolus injection, and the specific operations comprise:
performing a pre-use preparation operation: one of the first injector 3 and the second injector 4 is in a pushing shape, the other is in an extending shape and is full of buffer solution, wherein the buffer solution amount of the serum-containing sample in the injector is consistent with the buffer solution amount of the serum-containing sample to be pushed in the detection cartridge 1;
performing a bolus operation: tightening the first and second ejectors 3, 4 on the first and second ejector ports 13, 14, respectively, alternately pushing the first and second ejectors 3, 4 so that the buffer solution containing the serum sample flows back and forth in the test cartridge 1, repeatedly passing through the face of the middle test membrane 5, wherein the duration or number of ejectors depends on the test requirements;
performing a liquid removal operation: after the reaction is finished, the first injector 3 and the second injector 4 are removed, the first injector 13 and the second injector 14 are opened, and a buffer solution containing serum samples is removed by a suction tube or a water absorbing material prepared in advance;
5. washing the film:
5.1, injecting a preset reagent, namely washing liquid, into the accommodating cavity of the detection box 1 through a first pushing and injecting port 13 or a second pushing and injecting port 14 on the detection box 1, and filling all the spaces of the accommodating cavity with the washing liquid;
5.2 bolus injection the bolus injector is used for bolus injection, and the specific operations comprise:
performing a pre-use preparation operation: one of the first injector 3 and the second injector 4 is in a push shape, the other is in an extension shape and is full of the washing liquid, wherein the amount of the washing liquid in the injector is consistent with the amount of the washing liquid to be pushed in the detection cartridge 1;
performing a bolus operation: the first and second syringes 3 and 4 are alternately pushed by screwing the syringes 3 and 4 on the respective syringe ports 13 and 14, so that the washing liquid flows back and forth in the test cartridge 1, repeatedly passing through the surface of the test membrane 5 in between. Wherein the duration or number of bolus injections depends on the test requirements;
performing a liquid removal operation: after the reaction is finished, the first injector 3 and the second injector 4 are removed, the first injector 13 and the second injector 14 are opened, and washing liquid is removed by a suction tube or a water absorbing material prepared in advance;
6. and (3) detection:
6.1, adding a preset reagent, namely a fluorescence detection reagent (a fluorescence-labeled anti-pepsinogen antibody), into the accommodating cavity of the detection box 1 through a first pushing port 13 or a second pushing port 14 on the detection box 1, and filling all the spaces of the accommodating cavity with the fluorescence detection reagent;
6.2, performing a bolus operation: the injector is used for injecting, and specific operations comprise:
performing a pre-use preparation operation: causing one of the first and second syringes 3 and 4 to be pushed in a push shape and the other to be stretched and to be full of the fluorescent detection reagent, wherein the amount of the fluorescent detection reagent in the syringe is identical to the amount of the fluorescent detection reagent to be pushed in the detection cartridge 1;
performing a bolus operation: the first injector 3 and the second injector 4 are screwed on the first injector port 13 and the second injector port 14 respectively, and the first injector 3 and the second injector 4 are alternately pushed so that the fluorescence detection reagent flows back and forth in the detection cartridge 1 and repeatedly passes through the surface of the middle test membrane 5. Wherein the duration or number of bolus injections depends on the test requirements;
performing a liquid removal operation: after the reaction is finished, the first injector 3 and the second injector 4 are removed, the first injector 13 and the second injector 14 are opened, and a fluorescence detection reagent is removed by a suction tube or a water absorbing material prepared in advance;
7. washing the film:
7.1, filling the washing liquid into all spaces of the accommodating cavity of the detection box 1 through a first pushing and injecting port 13 or a second pushing and injecting port 14 on the detection box 1 to a reagent-washing liquid preset in the accommodating cavity of the detection box 1;
7.2, performing a bolus operation: the injector is used for injecting, and specific operations comprise:
performing a pre-use preparation operation: one of the first injector 3 and the second injector 4 is in a push shape, the other is in an extension shape and is full of the washing liquid, wherein the amount of the washing liquid in the injector is consistent with the amount of the washing liquid to be pushed in the detection cartridge 1;
performing a bolus operation: tightening the first and second ejectors 3, 4 on the first and second ejector ports 13, 14, respectively, alternately advancing the first and second ejectors 3, 4 such that the wash solution flows back and forth within the test cartridge 1, repeatedly across the face of the intermediate test membrane 5, wherein the duration or number of ejectors depends on the test requirements;
performing a liquid removal operation: after the reaction is finished, the first injector 3 and the second injector 4 are removed, the first injector 13 and the second injector 14 are opened, and washing liquid is removed by a suction tube or a water absorbing material prepared in advance;
8. reading the result: and obtaining a test result according to a related program.
It can be seen from the above two examples that the detection and analysis device and the corresponding detection and analysis system of the present invention can be applied in the operation steps of sealing the test membrane, washing the membrane, adding the sample, detecting, etc., and the specific implementation details of each operation step can also refer to each operation flow mentioned in the above general operation, and only needs to cooperate with the reagent actually required to be applied, the time of injection and the number of times of injection. Besides the two examples, the detection analysis device and the corresponding detection analysis system can be applied to other experiments requiring similar operations, are convenient to operate and effectively improve the detection efficiency.
Example 2
In this embodiment, the detection and analysis system may include the same detection and analysis device and test membrane 5 as in embodiment 1, and an automatic injection component may be added on the basis of embodiment 1, where the automatic injection component is connected to the injector, and the automatic injection component is an existing mechanism in the prior art, and may drive the injector to automatically inject and suck out the liquid, and the frequency and time of injection may be adjusted according to the detection requirement, so that the labor of manual injection may be avoided and the control accuracy may be improved by setting the automatic injection component.
In this embodiment, a heating mechanism is also provided in the automatic bolus assembly at a position in contact with the bolus, so that the liquid in the bolus can be heated while the bolus is being made to further enhance the reaction effect
The operation of the detection and analysis system in this embodiment is the same as that in embodiment 1, except that the manually operated portion is replaced with automatic control by the apparatus, so the operation will not be further described here.
The detection and analysis device and the corresponding detection and analysis system have the following characteristics:
the detection analysis device provides a new solution for optimizing and improving functions of detection reagents, and is helpful for solving some technical difficulties and limitations in the prior art. The detection and analysis system is a closed system: after the test membrane 5 is arranged, an upper cover and a lower cover of the detection box are covered, a pushing injector is screwed on a pushing injection port on the detection box, and the whole system is closed; the direction of the liquid flowing back and forth in the detection box forms 45-90 degrees with the surface of the test membrane 5; the membrane side is the only point through which fluid flows from one side of the membrane to the other; therefore, the working efficiency of the detection system can be effectively improved, and the liquid can not leak. The test membrane is arranged between the two pushing ports of the detection box, and liquid in the detection box can be repeatedly pushed and sucked out through the pushing device during use, so that the liquid repeatedly flows through the test membrane, the reagent is effectively promoted to pass through the porous solid-phase membrane, the reactivity of a detection test system is improved by increasing the contact frequency between active elements, and the detection efficiency is effectively improved. The detection and analysis device and the system comprising the detection and analysis device have the advantages of simple structure, convenient operation, low manufacturing cost, effective improvement of detection efficiency and wide application range.
The above embodiments present several preferred examples of the present invention in detail. It should be understood that numerous modifications and variations can be made in accordance with the concepts of the invention by one of ordinary skill in the art without undue burden. Therefore, all technical solutions which can be obtained by logic analysis, reasoning or limited experiments based on the prior art by the present invention by the person skilled in the art shall be within the scope of protection defined by the claims.
Claims (10)
1. The detection and analysis device is characterized by comprising a detection box, a membrane frame and a pushing device;
the detection box is internally provided with a containing cavity, and the outer surface of the detection box is provided with two pushing injection ports communicated with the containing cavity;
the membrane frame is used for fixing an external test membrane, is arranged in the accommodating cavity and is positioned between the two pushing injection ports, and an included angle between a plane formed by the membrane frame and the bottom surface of the accommodating cavity is 45-90 degrees;
the injector is detachably fixed at the injection port and is communicated with the accommodating cavity in the detection box through the injection port.
2. The detecting and analyzing device according to claim 1, wherein a film frame fixing groove is provided in the housing cavity, and the film frame is detachably inserted into the film frame fixing groove to be fixed in the housing cavity.
3. The detection and analysis device according to claim 1, wherein the detection and analysis device includes two of the ejectors, the ejectors being detachably connected to the two ejector ports, respectively.
4. The detection and analysis device according to claim 1, wherein the detection cartridge is constituted by an upper cover and a lower cover sealed closed, and the housing chamber is constituted by a space between the upper cover and the lower cover.
5. The assay device of claim 1, wherein the cartridge is made of a transparent material.
6. A detection and analysis system comprising the test membrane and the detection and analysis device according to any one of claims 1 to 5, wherein the test membrane is disposed in the housing cavity through the membrane frame.
7. The assay system of claim 6, further comprising a reagent injection assembly for injecting reagent into the receiving chamber through any one of the push ports.
8. The assay system of claim 6, wherein the reagent injection assembly is comprised of a pipette or a drip bottle.
9. The detection and analysis system of claim 6, further comprising an automatic bolus assembly coupled to the bolus.
10. The assay system of claim 9, wherein a heating mechanism is further provided in the automated bolus assembly at a location in contact with the bolus.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202210779792.1A CN117388482A (en) | 2022-07-04 | 2022-07-04 | Detection analysis device and corresponding detection analysis system |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202210779792.1A CN117388482A (en) | 2022-07-04 | 2022-07-04 | Detection analysis device and corresponding detection analysis system |
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| CN117388482A true CN117388482A (en) | 2024-01-12 |
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